BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	533,403	G→A	A326T (GCG→ACG)	unknown →	tRNA pseudouridine(13) synthase (EC 5.4.99.27)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	533,403	0	G	A	100.0%	311.2 / NA	104	A326T (GCG→ACG)	unknown	tRNA pseudouridine(13) synthase (EC 5.4.99.27)
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (53/51); total (53/51)

Only 100 of 104 total aligned reads displayed.

GCAACAACGCTTGGCACAACGGGATATTTTGCTTACCGCACCGCTTATCGGCGAAGAGGACAAAAGTGCGGTGGATTTTGAGAATGAAATTTTTGTCGCGCACCAAGCCTTGTTCCATTTGATGCGGCAAGAACGCGTGAAAGCCGCCCGCCGTCCGATTTTAATGCAGGCGCAACAGTTTCAATGGCAATTTGAACCGAACGGTTTGCGCCTTAAATTTTATTTGCCGGCAGGCAGTTACGCCACGGCGTTGGTACGCGAGCTGGTGAATGTTGAAAACTGAAAAACGAGAAGAAAAACAGGAATAACAAGAACATGAATATTTTATTAAGTAACGATGACGGCATTCACGCGCCGGGCATTCGTGTGATGGCAGAAGCATTGCGTAAGATTGCCAATGTGACCATCGTCGCGCCGGACAGCAACCGCAGCGCCGCCTCCAGTTCCTTAACCTTGGTGAAGCCGTTGTATCCGTTACATTTGGA  >  1/533156‑533640

gCAACAACGCTTGGCACAACGGGATATTTTGCTTACCGCACCGCTTATCGGCGAAGAGGACAAAAGTGCGGTGGATTTTGAGAATGAAATTTTTGTCGCGCACCAAGCCTTGTTCCATTTGATGCGGCAAGAACGCGTGAAAGCCGCCCGCCGTCCGATTTTAATGCAGGCGCAACAGTTTCAATGGCAATTTGAACCGAACGGTTTGCGCCTTAAATTTTATTTGCCGGCAGGCAGTTACGCCACGACGt                                                                                                                                                                                                                                            >  1:485275/1‑251 (MQ=255)

gCAACAACGCTTGGCACAACGGGATATTTTGCTTACCGCACCGCTTATCGGCGAAGAGGACAAAAGTGCGGTGGATTTTGAGAATGAAATTTTTGTCGCGCACCAAGCCTTGTTCCATTTGATGCGGCAAGAACGCGTGAAAGCCGCCCGCCGTCCGATTTTAATGCAGGCGCAACAGTTTCAATGGCAATTTGAACCGAACGGTTTGCGCCTTAAATTTTATTTGCCGGCAGGCAGTTACGCCACGACGt                                                                                                                                                                                                                                            >  1:180643/1‑251 (MQ=255)

 caacaaCGCTTGGCACAACGGGATATTTTGCTTACCGCACCGCTTATCGGCGAAGAGGACAAAAGTGCGGTGGATTTTGAGAATGAAATTTTTGTCGCGCACCAAGCCTTGTTCCATTTGATGCGGCAAGAACGCGTGAAAGCCGCCCGCCGTCCGATTTTAATGCAGGCGCAACAGTTTCAATGGCAATTTGAACCGAACGGTTTGCGCCTTAAATTTTATTTGCCGGCAGGCAGTTACGCCACGACGtt                                                                                                                                                                                                                                           >  1:208501/1‑251 (MQ=255)

     aacGCTTGGCACAACGGGATATTTTGCTTACCGCACCGCTTATCGGCGAAGAGGACAAAAGTGCGGTGGATTTTGAGAATGAAATTTTTGTCGCGCACCAAGCCTTGTTCCGTTTGATGCGGCAAGAACGCGTGAAAGCCGCCCGCCGTCCGATTTTAATGCAGGCGCAACAGTTTCAATGGCAATTTGAACCGAACGGTTTGCGCCTTAAATTTTATTTGCCGGCAGGCAGTTACGCCACGACGTTGGTa                                                                                                                                                                                                                                       <  1:217169/251‑1 (MQ=255)

      acGCTTGGCACAACGGGATATTTTGCTTACCGCACCGCTTATCGGCGAAGAGGACAAAAGTGCGGTGGATTTTGAGAATGAAATTTTTGTCGCGCACCAAGCCTTGTTCCATTTGATGCGGCAAGAACGCGTGAAAGCCGCCCGCCGTCCGATTTTAATGCAGGCGCAACAGTTTCAATGGCAATTTGAACCGAACGGTTTGCGCCTTAAATTTTATTTGCCGGCAGGCAGTTACGCCACGACGTTGGTa                                                                                                                                                                                                                                       >  1:442068/1‑250 (MQ=255)

         cTTGGCACAACGGGATATTTTGCTTACCGCACCGCTTATCGGCGAAGAGGACAAAAGTGCGGTGGATTTTGAGAATGAAATTTTTGTCGCGCACCAAGCCTTGTTCCATTTGATGCGGCAAGAACGCGTGAAAGCCGCCCGCCGTCCGATTTTAATGCAGGCGCAACAGTTTCAATGGCAATTTGAACCGAACGGTTTGCGCCTTAAATTTTATTTGCCGGCAGGCAGTTACGCCACGACGTTGGTAcgcg                                                                                                                                                                                                                                   <  1:781254/251‑1 (MQ=255)

             gCACAACGGGATATTTTGCTTACCGCACCGCTTATCGGCGAAGAGGACAAAAGTGCGGTGGATTTTGAGAATGAAATTTTTGTCGCGCACCAAGCCTTGTTCCATTTGATGCGGCAAGAACGCGTGAAAGCCGCCCGCCGTCCGATTTTAATGCAGGCGCAACAGTTTCAATGGCAATTTGAACCGAACGGTTTGCGCCTTAAATTTTATTTGCCGGCAGGCAGTTACGCCACGACGTTGGTACGCGAg                                                                                                                                                                                                                                 >  1:690818/1‑249 (MQ=255)

             gCACAACGGGATATTTTGCTTACCGCACCGCTTATCGGCGAAGAGGACAAAAGTGCGGTGGATTTTGAGAATGAAATTTTTGTCGCGCACCAAGCCTTGTTCCATTTGATGCGGCAAGAACGCGTGAAAGCCGCCCGCCGTCCGATTTTAATGCAGGCGCAACAGTTTCAATGGCAATTTGAACCGAACGGTTTGCGCCTTAAATTTTATTTGCCGGCAGGCAGTTACGCCACGACGTTGGTACGCGAGc                                                                                                                                                                                                                                <  1:167013/250‑1 (MQ=255)

             gCACAACGGGATATTTTGCTTACCGCACCGCTTATCGGCGAAGAGGACAAAAGTGCGGTGGATTTTGAGAATGAAATTTTTGTCGCGCACCAAGCCTTGTTCCATTTGATGCGGCAAGAACGCGTGAAAGCCGCCCGCCGTCCGATTTTAATGCAGGCGCAACAGTTTCAATGGCAATTTGAACCGAACGGTTTGCGCCTTAAATTTTATTTGCCGGCAGGCAGTTACGCCACGACGTTGGTACGCGAGc                                                                                                                                                                                                                                <  1:832111/250‑1 (MQ=255)

                caACGGGATATTTTGCTTACCGCACCGCTTATCGGCGAAGAGGACAAAAGTGCGGTGGATTTTGAGAATGAAATTTTTGTCGCGCACCAAGCCTTGTTCCATTTGATGCGGCAAGAACGCGTGAAAGCCGCCCGCCGTCCGATTTTAATGCAGGCGCAACAGTTTCAATAGCAATTTGAACCGAACGGTTTGCGCCTTAAATTTTATTTGCCGGCAGGCAGTTACGCCACGACGTTGGTACGCGAGCTGGt                                                                                                                                                                                                                            >  1:435265/1‑251 (MQ=255)

                 aaCGGGATATTTTGCTTACCGCACCGCTTATCGGCGAAGAGGACAAAAGTGCGGTGGATTTTGAGAATGAAATTTTTGTCGCGCACCAAGCCTTGTTCCATTTGATGCGGCAAGAACGCGTGAAAGCCGCCCGCCGTCCGATTTTAATGCAGGCGCAACAGTTTCAATGGCAATTTGAACCGAACGGTTTGCGCCTTAAATTTTATTTGCCGGCAGGCAGTTACGCCACGACGTTGGTACGCGAGCTGGTg                                                                                                                                                                                                                           <  1:496665/251‑1 (MQ=255)

                  aCGGGATATTTTGCTTACCGCACCGCTTATCGGCGAAGAGGTCAAAAGTGCGGTGGATTTTGAGAATGAAATTTTTGTCGCGCTCCAAGCCTTGTTCCATTTGATGCGGCAAGAACGCGTGAAAGCCGCCAGCCGTCCGATTTTAATGCAGGCGCAACAGTTTCAATGGCAATTTGAACCGAACGGTTTGTGCCTTAAATTTTATTTGCCGGCAGGCAGTTACGCCACGACGTTGGTACGCGAGCTGGTGa                                                                                                                                                                                                                          <  1:739741/251‑1 (MQ=255)

                  aCGGGATATTTTGCTTACCGCACCGCTTATCGGCGAAGAGGACAAAAGTGCGGTGGATTTTGAGAATGAAATTTTTGTTGCGCACCAAGCCTTGTTCCATTTGATGCGGCAAGAACGCGTGAAAGCCGCCCGCCGTCCGATTTTAATGCAGGCGCAACAGTTTCAATGGCAATTTGAACCGAACGGTTTGCGCCTTAAATTTTATTTGCCGGCAGGCAGTTACGCCACGACGTTGGTACGCGAGCTGGTGa                                                                                                                                                                                                                          <  1:878789/251‑1 (MQ=255)

                  aCGGGATATTTTGCTTACCGCACCGCTTATCGGCGAAGAGGACAAAAGTGCGGTGGATTTTGAGAATGAAATTTTTGTCGCGCACCAAGCCTTGTTCCATTTGATGCGGCAAGAACGCGTGAAAGCCGCCCGCCGTCCGATTTTAATGCAGGCGCAACAGTTTCAATGGCAATTTGAACCGAACGGTTTGCGCCTTAAATTTTATTTGCCGGCAGGCAGTTACGCCACGACGTTGGTACGCGAGCTGGTGa                                                                                                                                                                                                                          >  1:403527/1‑251 (MQ=255)

                       atatTTTGCTTACCGCACCGCTTATCGGCGAAGAGGACAAAAGTGCGGTGGATTTTGAGAATGAAATTTTTGTCGCGCACCAAGCCTTGTTCCATTTGATGCGGCAAGAACGCGTGAAAGCCGCCCGCCGTCCGATTTTAATGCAGGCGCAACAGTTTCAATGGCAATTTGAACCGAACGGTTTGCGCCTTAAATTTTATTTGCCGGCAGGCAGTTACGCCACGACGTTGGTa                                                                                                                                                                                                                                       >  1:752267/1‑233 (MQ=255)

                       atatTTTGCTTACCGCACCGCTTATCGGCGAAGAGGACAAAAGTGCGGTGGATTTTGAGAATGAAATTTTTGTCGCGCACCAAGCCTTGTTCCATTTGATGCGGCAAGAACGCGTGAAAGCCGCCCGCCGTCCGATTTTAATGCAGGCGCAACAGTTTCAATGGCAATTTGAACCGAACGGTTTGCGCCTTAAATTTTATTTGCCGGCAGGCAGTTACGCCACGACGTTGGTACGCGAGCTGGTGAATGtt                                                                                                                                                                                                                     >  1:36210/1‑251 (MQ=255)

                         atTTTGCTTACCGCACCGCTTATCGGCGAAGAGGACAAAAGTGCGGTGGATTTTGAGAATGAAATTTTTGTCGCGCACCAAGCCTTGTTCCATTTGATGCGGCAAGAACGCGTGAAAGCCGCCCGCCGTCCGATTTTAATGCAGGCGCAACAGTTTCAATGGCAATTTGAACCGAACGGTTTGCGCCTTAAATTTTATTTGCCGGCAGGCAGTTACGCCACGACGTTggga                                                                                                                                                                                                                                       >  1:805303/1‑229 (MQ=255)

                             tGCTTACCGCACCGCTTATCGGCGAAGAGGACAAAAGTGCGGTGGATTTTGAGAATGAAATTTTTGTCGCGCACCAAGCCTGGTTCCATTTGATGCGGCAAGAACGCGTGAAAGCCGCCCGCCGTCCGATTTTAATGCAGGCGCAACAGTTTCAATGGCAATTTGAACCGAACGGTTTGCGCCTTAAATTTTATTTGCCGGCAGGCAGTTACGCCACGACGTTGGTACGCGAGCTGGTGAATGTTGAAAAc                                                                                                                                                                                                               <  1:444128/251‑1 (MQ=255)

                                ttACCGCACCGCTTATCGGCGAAGAGGACAAAAGTGCGGTGGATTTTGAGAATGAAATTTTTGTCGCGCACCAAGCCTTGTTCCATTTGATGCGGCAAGAACGCGTGAAAGCCGCCCGCCGTCCGATTTTAATGCAGGCGCAACAGTTTCAATGGCAATTTGAACCGAACGGTTTGCGCCTTAAATTTTATTTGCCGGCAGGCAGTTACGCCACGACGTTGGTACGCGAGCTGGTGAATGTTGAAAACTGa                                                                                                                                                                                                            >  1:212117/1‑251 (MQ=255)

                                ttACCGCACCGCTTATCGGCGAAGAGGACAAAAGTGCGGTGGATTTTGAGAATGAAATTTTTGTCGCGCACCAAGCCTTGTTCCATTTGATGCGGCAAGAACGCGTGAAAGCCGCCCGCCGTCCGATTTTAATGCAGGCGCAACAGTTTCAATGGCAATTTGAACCGAACGGTTTGCGCCTTAAATTTTATTTGCCGGCAGGCAGTTACGCCACGACGATGGTACGCGAGCTGGTGAATGTTGAAAACTGa                                                                                                                                                                                                            >  1:685409/1‑251 (MQ=255)

                                   ccgcaccgcTTATCGGCGAAGAGGACAAAAGTGCGGTGGATTTTGAGAATGAAATTTTTGTCGCGCACCAAGCCTTGTTCCATTTGATGCGGCAAGAACGCGTGAAAGCCGCCCGCCGTCCGATTTTAATGCAGGCGCAACAGTTTCAATGGCAATTTGAACCGAACGGTTTGCGCCTTAAATTTTATTTGCCGGCAGGCAGTTACGCCACGACGTTGGTACGCGAGCTGGTGAATGTTGAAAACTGaaaa                                                                                                                                                                                                         >  1:212337/1‑251 (MQ=255)

                                     gcaccgcTTATCGGCGAAGAGGACAAAAGTGCGGTGGATTTTGAGAATGAAATTTTTGTCGCGCACCAAGCCTTGTTCCATTTGATGCGGCAAGAACGCGTGAAAGCCGCCCGCCGTCCGATTTTAATGCAGGCGCAACAGTTTCAATGGCAATTTGAACCGAACGGTTTGCGCCTTAAATTTTATTTGCCGGCAGGCAGTTACGCCACGACGTTGGTACGCGAGCTGGTGAATGTTGAAAACTGaaaaa                                                                                                                                                                                                        >  1:753377/1‑250 (MQ=255)

                                        ccgcTTATCGGCGAAGAGGACAAAAGTGCGGTGGATTTTGAGAATGAAATTTTTGTCGCGCACCAAGCCTTGTTCCATTTGATGCGGCAAGAACGCGTGAAAGCCGCCCGCCGTCCGATTTTAATGCAGGCGCAACAGTTTCAATGGCAATTTGAACCGAACGGTTTGCGCCTTAAATTTTATTTGCCGGCAGGCAGTTACGCCACGACGTTGGTACGCGAGCTGGTGAATGTTGAAAACTGAAAAACgag                                                                                                                                                                                                    >  1:399551/1‑251 (MQ=255)

                                          gcTTATCGGCGAAGAGGACAAAAGTGCGGTGGATTTTGAGAATGAAATTTTTGTCGCGCACCAAGCCTTGTTCCATTTGATGCGGCAAGAACGCGTGAAAGCCGCCCGCCGTCCGATTTTAATGCAGGCGCAACAGTTTCAATGGCAATTTGAACCGAACGGTTTGCGCCTTAAATTTTATTTGCCGGCAGGCAGTTACGCCACGACGTTGGTACGCGAGCTGGTGAATGTTGAAAACTGAAAAACGagaa                                                                                                                                                                                                  >  1:137389/1‑251 (MQ=255)

                                          gcTTATCGGCGAAGAGGACAAAAGTGCGGTGGATTTTGAGAATGAAATTTTTGTCGCGCACCAAGCCTTGTTCCATTTGATGCGGCAAGAACGCGTGAAAGCCGCCCGCCGTCCGATTTTAATGCAGGCGCAACAGTTTCAATGGCAATTTGAACCGAACGGTTTGCGCCTTAAATTTTATTTGCCGGCAGGCAGTTACGCCACGACGTTGGTACGCGAGCTGGTGAATGTTGAAAACTGAAAAACGagaa                                                                                                                                                                                                  >  1:824435/1‑251 (MQ=255)

                                           cTTATCGGCGAAGAGGACAAAAGTGCGGTGGATTTTGAGAATGAAATTTTTGTCGCGCACCAAGCCTTGTTCCATTTGATGTGGCAAGAACGCGTGAAAGCCGCCCGCCGTCCGATTTTAATGCAGGCGCAACAGTTTCAATGGCAATTTGAACCGAACGGTTTGCGCCTTAAATTTTATTTGCCGGCAGGCAGTTACGCCACGACGTTGGTACGCGAGCTGGTGAATGTTGAAAACTGAAAAACGagaag                                                                                                                                                                                                 <  1:435490/251‑1 (MQ=255)

                                                       gagGACAAAAGTGCGGTGGATTTTGAGAATGAAATTTTTGTCGCGCACCAAGCCTTGTTCCATTTGATGCGGCAAGAACGCGTGAAAGCCGCCCGCCGTCCGATTTTAATGCAGGCGCAACAGTTTCAATGGCAATTTGAACCGAACGGTTTGCGCCTTAAATTTTATTTGCCGGCAGGCAGTTACGCCACGACGTTGGTACGCGAGCTGGTGAATGTTGAAAACTGAAAAACGAGAAGAAAAACAGGAAt                                                                                                                                                                                     >  1:79649/1‑251 (MQ=255)

                                                         ggACAAAAGTGCGGTGGATTTTGAGAATGAAATTTTTGTCGCGCACCAAGCCTTGTTCCATTTGATGCGGCAAGAACGCGTGAAAGCCGCCCGCCGTCCGATTTTAATGCAGGCGCAACAGTTTCAATGGCAATTTGAACCGAACGGTTTGCGCCTTAAATTTTATTTGCCGGCAGGCAGTTACGCCACGACGTTGGTACGCGAGCTGGTGAATGTTGAAAACTGAAAAACGAGAAGAAAAACAGGAATaa                                                                                                                                                                                   >  1:890766/1‑251 (MQ=255)

                                                              aaaGTGCGGTGGATTTTGAGAATGAAATTTTTGTCGCGCACCAAGCCTTGTTCCATTTGATGCGGCAAGAACGCGTGAAAGCCGCCCGCCGTCCGATTTTAATGCAGGCGCAACAGTTTCAATGGCAATTTGAACCGAACGGTTTGCGCCTTAAATTTTATTTGCCGGCGGGCAGTTACGCCACGACGTTGGTACGCGAGCTGGTGAATGTTGAAAACTGAAAAACGAGAAGAAAAACAGGAATAACAAGa                                                                                                                                                                              >  1:444472/1‑251 (MQ=255)

                                                              aaaGTGCGGTGGATTTTGAGAATGAAATTTTTGTCGCGCACCAAGCCTTGTTCCATTTGATGCGGCAAGAACGCGTGAAAGCCGCCCGCCGTCCGATTTTAATGCAGGCGCAACAGTTTCAATGGCAATTTGAACCGAACGGTTTGCGCCTTAAATTTTATTTGCCGGCAGGCAGTTACGCCACGACGTTGGTACGCGAGCTGGTGAATGTTGAAAACTGAAAAACGAGAAGAAAAACAGGAATAACAAGa                                                                                                                                                                              >  1:767344/1‑251 (MQ=255)

                                                               aaGTGCGGTGGATTTTGAGAATGAAATTTTTGTCGCGCACCAAGCCTTGTTCCATTTGATGCGGCAAGAACGCGTGAAAGCCGCCCGCCGTCCGATTTTAATGCAGGCGCAACAGTTTCAATGGCAATTTGAACCGAACGGTTTGCGCCTTAAATTTTATTTGCCGGCAGGCAGTTACGCCACGACGTTGGTACGCGAGCTGGTGAATGTTGAAAAc                                                                                                                                                                                                               <  1:696647/217‑1 (MQ=255)

                                                                     ggTGGATTTTGAGAATGAAATTTTTGTCGCGCACCAAGCCTTGTTCCATTTGATGCGGGAAGAACGCGTGAAAGCCGCCCGCCGTCCGATTTTAATGCAGGCGCAACAGTTTCAATGGCAATTTGAACCGAACGGTTTGCGCCTTAAATTTTATTTGCCGGCAGGCAGTTACGCCACGACGTTGGTACGCGAGCTGGTGAATGTTGAAAACTGAAAAACGAGAAGAAAAACAGGAATAACAAGAACATGaa                                                                                                                                                                       >  1:568307/1‑251 (MQ=255)

                                                                      gTGGATTTTGAGAATGAAATTTTTGTCGCGCACCAAGCCTTGTTCCATTTGATGCGGCAAGAACGCGTGAAAGCCGCCCGCCGTCCGATTTTAATGCAGGCGCAACAGTTTCAATGGCAATTTGAACCGAACGGTTTGCGCCTTAAATTTTATTTGCCGGCAAGCAGTTACGCCACGACGTTGGTACGCGAGCTGGTGAATGTTGAAAACTGAAAAACGAGAAGAAAAACAGGAATAACAAGAACATGAat                                                                                                                                                                      >  1:103239/1‑251 (MQ=255)

                                                                       tGGATTTTGAGAATGAAATTTTTGTCGCGCACCAAGCCTTGTTCCATTTGATGCGGCAAGAACGCGTGAAAGCCGCCCGCCGTCCGATTTTAATGCAGGCGCAACAGTTTCAATGGCAATTTGAACCGAACGGTTTGCGCCTTAAATTTTATTTGCCGGCAGGCAGTTACGCCACGACGTTGGTACGCGAGCTGGTGAATGTTGAAAACTGAAAAACGAGAAGAAAAACAGGAATAACAAGAACATGAata                                                                                                                                                                     <  1:86380/251‑1 (MQ=255)

                                                                         gATTTTGAGAATGAAATTTTTGTCGCGCACCAAGCCTTGTTCCATTTGATGCGGCAAGAACGCGTGAAAGCCGCCCGCCGTCCGATTTTAATGCAGGCGCAACAGTTTCAATGGCAATTTGAACCGAACGGTTTGCGCCTTAAATTTTATTTGCCGGCAGGCAGTTACGCCACGACGTTGGTACGCGAGCTGGTGAATGTTGAAAACTGAAAAACGAGAAGAAAAACAGGAATAACAAGAACATGAatat                                                                                                                                                                    <  1:587598/250‑1 (MQ=255)

                                                                         gATTTTGAGAATGAAATTTTTGTCGCGCACCAAGCCTTGTTCCATTTGATGCGGCAAGAACGCGTGAAAGCCGCCCGCCGTCCGATTTTAATGCAGGCGCAACAGTTTCAATGGCAATTTGAACCGAACGGTTTGCGCCTTAAATTTTATTTGCCGGCAGGCAGTTACGCCACGACGTTGGTACGCGAGCTGGTGAATGTTGAAAACTGAAAAACGAGAAGAAAAACAGGAATAACAAGAACATGAATAtt                                                                                                                                                                   >  1:535543/1‑251 (MQ=255)

                                                                             ttGAGAATGAAATTTTTGTCGCGCACCAAGCCTTGTTCCATTTGATGCGGCAAGAACGCGTGAAAGCCGCCCGCCGTCCGATTTTAATGCAGGCGCAACAGTTTCAATGGCAATTTGAACCGAACGGTTTGCGCCTTAAATTTTATTTGCCGGCAGGCAGTTACGCCACGACGTTGGTACGCGAGCTGGTGAATGTTGAAAACTGAAAAACGAGAAGAAAAACAGGAATAACAAGAACATGAATATTttat                                                                                                                                                               <  1:153386/251‑1 (MQ=255)

                                                                                 gaATGAAATTTTTGTCGCGCACCAAGCCTTGTTCCATTTGATGCGGCAAGAACGCGTGAAAGCCGCCCGCCGTCCGATTTTAATGCAGGCGCAACAGTTTCAATGGCAATTTGAACCGAACGGTTTGCGCCTTAAATTTTATTTGCCGGCAGGCAGTTACGCCACGACGTTGGTACGCGAGCTGGTGAATGTTGAAAACTGAAAAACGAGAAGAAAAACAGGAATAACAAGAACATGAATATTTTATTAAg                                                                                                                                                           >  1:780386/1‑251 (MQ=255)

                                                                                  aaTGAAATTTTTGTCGCGCACCAGGCCTTGTTCCATTTGATGCGGCAAGAACGCGTGAAAGCCGCCCGCCGTCCGATTTTAATGCAGGCGCAACAGTTTCAATGGCAATTTGAACCGAACGGTTTGCGCCTTAAATTTTATTTGCCGGCAGGCAGTTACGCCACGACGTTGGTACGCGAGCTGGTGAATGTTGAAAACTGAAAAACGAGAAGAAAAACAGGAATAACAAGAACATGAATATTTTATTAAGt                                                                                                                                                          <  1:2890/251‑1 (MQ=255)

                                                                                    tGAAATTTTTGTCGCGCACCAAGCCCTGTTCCATTTGATGCGGCAAGAACGCGTGAAAGCCGCCCGCCGTCCGATTTTAATGCAGGCGCAACAGTTTCAATGGCAATTTGAACCGAACGGTTTGCGCCTTAAATTTTATTTGCCGGCAGGCAGTTACGCCACGACGTTGGTACGCGAGCTGGTGAATGTTGAAAACTGAAAAACGAGAAGAAAAACAGGAATAACAAGAACATGAATATTTTATTAAGTaa                                                                                                                                                        <  1:223455/251‑1 (MQ=255)

                                                                                       aaTTTTTGTCGCGCACCAAGCCTTGTTCCATTTGATGCGGCAAGAACGCGTGAAAGCCGCCCGCCGTCCGATTTTAATGCAGGCGCAACAGTTTCAATGGCAATTTGAACCGAACGGTTTGCGCCTTAAATTTTATTTGCCGGCAGGCAGTTACGCCACGACGTTGGTACGCGAGCTGGTGAATGTTGAAAACTGAAAAACGAGAAGAAAAACAGGAATAACAAGAACATGAATATTTTATTAAGTAACGa                                                                                                                                                     <  1:883874/251‑1 (MQ=255)

                                                                                                   gcACCAAGCCTTGTTCCATTTGATGCGGCAAGAACGCGTGAAAGCCGCCCGCCGTCCGATTTTAATGCAGGCGCAACAGTTTCAATGGCAATTTGAACCGAACGGTTTGCGCCTTAAATTTTATTTGCCGGCAGGCAGTTACGCCACGACGTTGGTACGCGAGCTGGTGAATGTTGAAAACTGAAAAACGAGAAGAAAAACAGGAATAACAAGAACATGAATATTTTATTAAGTAACGATGACGGCATTCa                                                                                                                                         >  1:827852/1‑251 (MQ=255)

                                                                                                    cACCAAGCCTTGTTCCATTTGATGCGGCAAGAACGCGTGAAAGCCGCCCGCCGTCCGATTTTAATGCAGGCGCAACAGTTTCAATGGCAATTTGAACCGAACGGTTTGCGCCTTAAATTTTATTTGCCGGCAGGCAGTTACGCCACGACGTTGGTACGCGAGCTGGTGAATGTTGAAAACTGAAAAACGAGAAGAAAAACAGGAATAACAAGAACATGAATATTTTATTAAGTAACGATGACGGCATTCa                                                                                                                                         >  1:159876/1‑250 (MQ=255)

                                                                                                    cACCAAGCCTTGTTCCATTTGATGCGGCAAGAACGCGTGAAAGCCGCCCGCCGTCCGATTTTAATGCAGGCGCAACAGTTTCAATGGCAATTTGAACCGAACGGTTTGCGCCTTAAATTTTATTTGCCGGCAGGCAGTTACGCCACGACGTTGGTACGCGAGCTGGTGAATGTTGAAAACTGAAAAACGAGAAGAAAAACAGGAATAACAAGAACATGAATATTTTATTAAGTAACGATGACGGCATTCa                                                                                                                                         <  1:572066/250‑1 (MQ=255)

                                                                                                     cccAAGCCTTGTTCCATTTGATGCGGCAAGAACGCGTGAAAGCCGCCCGCCGTCCGATTTTAATGCAGGCGCAACAGTTTCAATGGCAATTTGAACCGAACGGTTTGCGCCTTAAATTTTATTTGCCGGCAGGCAGTTACGCCACGACGTTGGTACGCGAGCTGGTGAATGTTGAAAACTGAAAAACGAGAAGAAAAACAGGAATAACAAGAACATGAATATTTTATTAAGTAACGATGACGGCATTCAcg                                                                                                                                       <  1:424065/250‑1 (MQ=255)

                                                                                                        aaGCCTTGTTCCATTTGATGCGGCAAGAACGCGTGAAAGCCGCCCGCCGTCCGATTTTAATGCAGGCGCAACAGTTTCAATGGCAATTTGAACCGAACGGTTTGCGCCTTAAATTTTATTTGCCGGCAGGCAGTTACGCCACGACGTTGGTACGCGAGCTGGTGAATGTTGAAAACTGAAAAACGAGAAGAAAAACAGGAATAACAAGAACATGAATATTTTATTAAGTAACGATGACgg                                                                                                                                               <  1:582974/240‑1 (MQ=255)

                                                                                                        aaGCCTTGTTCCATTTGATGCGGCAAGAACGCGTGAAAGCCGCCCGCCGTCCGATTTTAATGCAGGCGCAACAGTTTCAATGGCAATTTGAACCGAACGGTTTGCGCCTTAAATTTTATTTGCCGGCAGGCAGTTACGCCACGACGTTGGTACGCGAGCTGGTGAATGTTGAAAACTGAAAAACGAGAAGAAAAACAGGAATAACAAGAACATGAATATTTTATTAAGTAACGATGACGGCATTCAcgcg                                                                                                                                     >  1:565357/1‑250 (MQ=255)

                                                                                                          gCCTTGTTCCATTTGATGCGGCAAGAACGCGTGAAAGCCGCCCGCCGTCCGATTTTAATGCAGGCGCAACAGTTTCAATGGCAATTTGAACCGAACGGTTTGCGCCTTAAATTTTATTTGCCGGCAGGCAGTTACGCCACGACGTTGGTACGCGAGCTGGTGAATGTTGAAAACTGAAAAACga                                                                                                                                                                                                     >  1:336095/1‑184 (MQ=255)

                                                                                                          gCCTTGTTCCATTTGATGCGGCAAGAACGCGTGAAAGCCGCCCGCCGTCCGATTTTAATGCAGGCGCAACAGTTTCAATGGCAATTTGAACCGAACGGTTTGCGCCTTAAATTTTATTTGCCGGCAGGCAGTTACGCCACGACGTTGGTACGCGAGCTGGTGAATGTTGAAAACTGAAAAACGAGAAGAAAAACAGGAATAACAAGAACATGAATATTTTATTAAGTAACGATGACGGCATTCACGCGCCg                                                                                                                                  >  1:669705/1‑251 (MQ=255)

                                                                                                          gCCTTGTTCCATTTGATGCGGCAAGAACGCGTGAAAGCCGCCCGCCGTCCGATTTTAATGCAGGCGCAACAGTTTCAATGGCAATTTGAACCGAACGGTTTGCGCCTTAAATTTTATTTGCCGGCAGGCAGTTACGCCACGACGTTGGTACGCGAGCTGGTGAATGTTGAAAACTGAAAAACGAGAAGAAAAACAGGAATAACAAGAACATGAATATTTTATTAAGTAACGATGACGGCATTCACGCGCCg                                                                                                                                  >  1:780452/1‑251 (MQ=255)

                                                                                                          gCCTTGTTCCATTTGATGCGGCAAGAACGCGTGAAAGCCGCCCGCCGTCCGATTTTAATGCAGGCGCAACAGTTTCAATGGCAATTTGAACCGAACGGTTTGCGCCTTAAATTTTATTTGCCGGCAGGCAGTTACGCCACGACGTTGGTACGCGAGCTGGTGAATGTTGAAAACTGAAAAACGAGAAGAAAAACAGGAATAACAAGAACATGAATATTTTATTAAGTAACGATGACGGCATTCACGCGCCg                                                                                                                                  >  1:898559/1‑251 (MQ=255)

                                                                                                          gCCTTGTTCCATTTGATGCGGCAAGAACGCGTGAAAGCCGCCCGCCGTCCGATTTTAATGCAGGCGCAACAGTTTCAATGGCAATTTGAACCGAACGGTTTGCGCCTTAAATTTTATTTGCCGGCAGGCAGTTACGCCACGACGTTGGTACGCGAGCTGGTGAATGTTGAAAACTGAAAAACGAGAAGAAAAACAGGAATAACAAGAACATGAATATTTTATTAAGTAACGATGACGGCATTCACGCGCCg                                                                                                                                  >  1:872316/1‑251 (MQ=255)

                                                                                                            cTTGTTCCATTTGATGCGGCAAGAACGCGTGAAAGCCGCCCGCCGTCCGATTTTAATGCAGGCGCAACAGTTTCAATGGCAATTTGAACCGAACGGTTTGCGCCTTAAATTTTATTTGCCGGCAGGCAGTTACGCCACGACGTTGGTACGCGAGCTGGTGAATGTTGAAAACTGAAAAACGAGAAGAAAAACAGGAATAACAAGAACATGAATATTTTATTAAGTAACGATGACGGCATTCACGCGCCggg                                                                                                                                <  1:16217/251‑1 (MQ=255)

                                                                                                            cTTGTTCCATTTGATGCGGCAAGAACGCGTGAAAGCCGCCCGCCGTCCGATTTTAATGCAGGCGCAACAGTTTCAATGGCAATTTGAACCGAACGGTTTGCGCCTTAAATTTTATTTGCCGGCAGGCAGTTACGCCACGACGTTGGTACGCGAGCTGGTGAATGTTGAAAACTGAAAAACGAGAAGAAAAACAGGAATAACAAGAACATGAATATTTTATTAAGTAACGATGACGGCATTCACGCGCCggg                                                                                                                                <  1:390704/251‑1 (MQ=255)

                                                                                                               gTTCCATTTGATGCGGCAAGAACGCGTGAAAGCCGCCCGCCGTCCGATTTTAATGCAGGCGCAACAGTTTCAATGGCAATTTGAACCGAACGGTTTGCGCCTTAAATTTTATTTGCCGGCAGGCAGTTACGCCACGACGTTGGTACGCGAGCTGGTGAATGTTGAAAACTGAAAAACGAGAAGAAAAACAGGAATAACAAGAACATGAATATTTTATTAAGTAACGATGACGGCATTCACGCGCCGGGCAt                                                                                                                             >  1:780675/1‑251 (MQ=255)

                                                                                                                  ccATTTGATGCGGCAAGAACGCGTGAAAGCCGCCCGCCGTCCGATTTTAATGCAGGCGCAACAGTTTCAATGGCAATTTGAACCGAACGGTTTGCGCCTTAAATTTTATTTGCCGGCAGGCAGTTACGCCACGACGTTGGTACGCGAGCTGGTGAATGTTGAAAACTGAAAAACGAGAAGAAAAACAGGAATAACAAGAACATGAATATTTTATTAAGTAACGATGACGGCATTCACGCGCCGGGCATTCg                                                                                                                          <  1:568956/251‑1 (MQ=255)

                                                                                                                  ccATTTGATGCGGCAAGAACGCGTGAAAGCCGCCCGCCGTCAGATTTTAATGCAGGCGCAACAGTTTCAATGGCAATTTGAACCGAACGGTTTGCGCCTTAAATTTTATTTGCCGGCAGGCAGTTACGCCACGACGTTGGTACGCGAGCTGGTGAATGt                                                                                                                                                                                                                      >  1:723015/1‑159 (MQ=255)

                                                                                                                    aTTTGATGCGGCAAGAACGCGTGAAAGCCGCCCGCCGTCCGATTTTAATGCAGGCGCAACAGTTTCAATGGCAATTTGAACCGAACGGTTTGCGCCTTAAATTTTATTTGCCGGCAGGCAGTTACGCCACGACGTTGGTACGCGAGCTGGTGAATGTTGAAAACTGAAAAACGAGAAGAAAAACAGGAATAACAAGAACATGAATATTTTATTAAGTAACGATGACGGCATTCACGCGCCGGGCATTCgtg                                                                                                                        >  1:124375/1‑251 (MQ=255)

                                                                                                                       tGATGCGGCAAGAACGCGTGAAAGCCGCCCGCCGTCCGATTTTAATGCAGGCGCAACAGTTTCAATGGCAATTTGAACCGAACGGTTTGCGCCTTAAATTTTATTTGCCGGCAGGCAGTTACGCCACGACGTTGGTACGCGAGCTGGTGAATGTTGAAAACTGAAAAACGAGAAGAAAAACAGGAATAACAAGAACATGAATATTTTATTAAGTAACGATGACGGCATTCACGCGCCGGGCATTCGTGTGa                                                                                                                     <  1:31352/251‑1 (MQ=255)

                                                                                                                        gATGCGGCAAGAACGCGTGAAAGCCGCCCGCCGTCCGATTTTAATGCAGGCGCAACAGTTTCAATGGCAATTTGAACCGAACGGTTTGCGCCTTAAATTTTATTTGCCGGCAGGCAGTTACGCCACGACGTTGGTACGCGAGCTGGTGAATGTTGAAAACTGAAAAACGAGAAGAAAAACAGGAATAACAAGAACATGaa                                                                                                                                                                       <  1:164807/200‑1 (MQ=255)

                                                                                                                          tGCGGCAAGAACGCGTGAAAGCCGCCCGCCGTCCGATTTTAATGCAGGCGCAACAGTTTCAATGGCAATTTGAACCGAACGGTTTGCGCCTTAAGTTTTATTTGCCGGCAGGCAGTTACGCCACGACGTTGGTACGCGAGCTGGTGAATGTTGAAAACTGAAAAACGAGAAGAAAAACAGGAATAACAAGAACATGAATATTTTATTAAGTAACGATGACGGCATTCACGCGCCGGGCATTCGTGTGATgg                                                                                                                  <  1:163448/251‑1 (MQ=255)

                                                                                                                                   aaCGCGTGAAAGCCGCCCGCCGTCCGATTTTAATGCAGGCGCAACAGTTTCAATGGCAATTTGAACCGAACGGTTTGCGCCTTAAATTTTATTTGCCGGCAGGCAGTTACGCCACGACGTTGGTACGCGAGCTGGTGAATGTTGAAAACTGAAAAACGAGAAGAAAAACAGGAATAACAAGAACATGAATATTTTATTAAGTAACGATGACGGCATTCACGCGCCGGGCATTCGTGTGATGGCAGAAGCAt                                                                                                         <  1:581168/251‑1 (MQ=255)

                                                                                                                                               ccgcccgcCGTCCGATTTTAATGCAGGCGCAACAGTTTCAATGGCAATTTGAACCGAACGGTTTGCGCCTTAAATTTTATTTGCCGGCAGGCAGTTACGCCACGACGTTGGTACGCGAGCTGGTGAATGTTGAAAACTGAAAAACGAGAAGAAAAACAGGAATAACAAGAACATGAATATTTTATTAAGTAACGATGACGGCATTCACGCGCCGGGCATTCGTGTGATGGCAGAAGCATTGCGTAAGAt                                                                                               <  1:124655/249‑1 (MQ=255)

                                                                                                                                               ccgcccgcCGTCCGATTTTAATGCAGGCGCAACAGTTTCAATGGCAATTTGAACCGAACGGTTTGCGCCTTAAATTTTATTTGCCGGCAGGCAGTTACGCCACGACGTTGGTACGCGAGCTGGTGAATGTTGAAAACTGAAAAACGAGAAGAAAAACAGGAATAACAAGAACATGAATATTTTATTAAGTAACGATGACGGCATTCACGCGCCGGGCATTCGTGTGATGGCAGAAGCATTGCGTAAGAt                                                                                               <  1:416152/249‑1 (MQ=255)

                                                                                                                                               ccgcccgcCGTCCGATTTTAATGCAGGCGCAACAGTTTCAATGGCAATTTGAACCGAACGGTTTGCGCCTTAAATTTTATTTGCCGGCAGGCAGTTACGCCACGACGTTGGTACGCGAGCTGGTGAATGTTGAAAACTGAAAAACGAGAAGAAAAACAGGAATAACAAGAACATGAATATTTTATTAAGTAACGATGACGGCATTCACGCGCCGGGCATTCGTGTGATGGCAGAAGCATTGCGTAAGAt                                                                                               <  1:56482/249‑1 (MQ=255)

                                                                                                                                                        gTCCGATTTTAATGCAGGCGCAACAGTTTCAATGGCAATTTGAACCGAACGGTTTGCGCCTTAAATTTTATTTGCCGGCAGGCAGTTACGCCACGACGTTGGTACGCGAGCTGGTGAATGTTGAAAACTGAAAAACGAGAAGAAAAACAGGAATAACAAGAACATGAATATTTTATTAAGTAACGATg                                                                                                                                                   >  1:306729/1‑188 (MQ=255)

                                                                                                                                                             aTTTTAATGCAGGCGCAACAGTTTCAATGGCAATTTGAACCGAACGGTTTGCGCCTTAAATTTTATTTGCCGGCAGGCAGTTACGCCACGACGTTGGTACGCGAGCTGGTGAATg                                                                                                                                                                                                                       <  1:777468/115‑1 (MQ=255)

                                                                                                                                                             aTTTTAATGCAGGCGCAACAGTTTCAATGGCAATTTGAACCGAACGGTTTGCGCCTTAAATTTTATTTGCCGGCAGGCAGTTACGCCACGACGTTGGTACGCGAGCTGGTGAATGTTGAAAACTGAAAAACGAGAAGAAAAACAGGAATAACa                                                                                                                                                                                 <  1:51205/153‑1 (MQ=255)

                                                                                                                                                             aTTTTAATGCAGGCGCAACAGTTTCAATGGCAATTTGAACCGAACGGTTTGCGCCTTAAATTTTATTTGCCGGCAGGCAGTTACGCCACGACGTTGGTACGCGAGCTGGTGAATGTTGAAAACTGAAAAACGAGAAGAAAAACAGGAATAACAAGAACATGAATATTTTATTAAGTAACGATGACGGCATTCACGCGCCGGGCAtt                                                                                                                            <  1:851859/206‑1 (MQ=255)

                                                                                                                                                             aTTTTAATGCAGGCGCAACAGTTTCAATGGCAATTTGAACCGAACGGTTTGCGCCTTAAATTTTATTTGCCGGCAGGCAGTTACGCCACGACGTTGGTACGCGAGCTGGTGAATGTTGAAAACTGAAAAACGAGAAGAAAAACAGGAATAACAAGAACATGAATATTTTATTAAGTAACGATGACGGCATTCACGCGCCGGGCATTCGTGTGATGGCAGAAGCATTGCGTAAGATTGCCAATGTGACCAt                                                                                >  1:474150/1‑250 (MQ=255)

                                                                                                                                                              ttttAATGCAGGCGCAACAGTTTCAATGGCAATTTGAACCGAACGGTTTGCGCCTTAAATTTTATTTGCCGGCAGGCAGTTACGCCACGACGTTGGTACGCGAGCTGGCGAATGTTGAAAACTGAAAAACGAGAAGAAAAACAGGAATAACAAGAACATGAATATTTTATTAAGTAACGATGACGGCATTCACGCGCCGGGCATTCGTGTGATGGCAGAAGCATTGCGTAAGATTGCCAATGTGACCAtcg                                                                              >  1:559947/1‑251 (MQ=255)

                                                                                                                                                               tttAATGCAGGCGCAACAGTTTCAATGGCAATTTGAACCGAACGGTTTGCGCCTTAAATTTTATTTGCCGGCAGGCAGTTACGCCACGACGTTGGTACGCGAGCTGGTGAATGTTGAAAACTGAAAAACGAGAAGAAAAACAGGAATAACAAGAACATGAATATTTTATTAAGTAACGATGACGGCATTCACGCGCCGGGCATTCGTGTGATGGCAGAAGCATTGCGTAAGATTGCCAATGTGACCAtcgt                                                                             <  1:179154/251‑1 (MQ=255)

                                                                                                                                                                       aGGCGCAACAGTTTCAATGGCAATTTGAACCGAACGGTTTGCGCCTTAAATTTTATTTGCCGGCAGGCAGTTACGCCACGACGTTGGTACGCGAGCTGGTGAATGTTGAAAACTGAAAAACGAGAAGAAAAACAGGAATAACAAGAACATGAATATTTTATTAAGTAACGATGACGGCATTCACGCGCCGGGCATTCGTGTGATGGCAGAAGCATTGCGTAAGAt                                                                                               >  1:816810/1‑225 (MQ=255)

                                                                                                                                                                          cgcAACAGTTTCAATGGCAATTTGAACCGAACGGTTTGCGCCTTAAATTTTATTTGCCGGCAGGCAGTTACGCCACGACGTTGGTACGCGAGCTGGTGAATGTTGAAAACTGAAAAACGAGAAGAAAAACAGGAATAACAAGAACATGAATATTTTATTAAGTAACGATGACGGCATTCACGCGCCGGGCATTCGTGTGATGGCAGAAGCATTGCGTAAGATTGCCAATGTGACCATCGTCGCGCCGGAc                                                                   <  1:496272/250‑1 (MQ=255)

                                                                                                                                                                          cgcAACAGTTTCAATGGCAATTTGAACCGAACGGTTTGCGCCTTAAATTTTATTTGCCGGCAGGCAGTTACGCCACGACGTTGGTACGCGAGCTGGTGAATGTTGAAAACTGAAAAACGAGAAGAAAAACAGGAATAACAAGAACATGAATATTTTATTAAGTAACGATGACGGCATTCACGCGCCGGGCATTCGTGTGATGGCAGAAGCATTGCGTAAGATTGCCAATGTGACCATCGTCGCGCCGGAc                                                                   <  1:588788/250‑1 (MQ=255)

                                                                                                                                                                            cAACAGTTTCAATGGCAATTTGAACCGAACGGTTTGCGCCTTAAATTTTATTTGCCGGCAGGCAGTTACGCCACGACGTTGGTACGCGAGCTGGTGAATGTTGAAAACTGAAAAACGAGAAGAAAAACAGGAATAACAAGAACATGAATATTTTATTAAGTAACGa                                                                                                                                                     <  1:154506/166‑1 (MQ=255)

                                                                                                                                                                            cAACAGTTTCAATGGCAATTTGAACCGAACGGTTTGCGCCTTAAATTTTATTTGCCGGCAGGCAGTTACGCCACGACGTTGGTACGCGAGCTGGTGAATGTTGAAAACTGAAAAACGAGAAGAAAAACAGGAATAACAAGAACATGAATATTTTATTAAGTAACGATGACGGCATTCACGCGCCGGGCATTCGTGTGATGGCAGAAGCATTGCGTAAGATTGCCAATGTGACCATCGTCGCGCCGGACAg                                                                 >  1:219966/1‑250 (MQ=255)

                                                                                                                                                                            cAACAGTTTCAATGGCAATTTGAACCGAACGGTTTGCGCCTTAAATTTTATTTGCCGGCAGGCAGTTACGCCACGACGTTGGTACGCGAGCTGGTGAATGTTGAAAACTGAAAAACGAGAAGAAAAACAGGAATAACAAGAACATGAATATTTTATTAAGTAACGATGACGGCATTCACGCGCCGGGCATTCGTGTGATGGCAGAAGCATTGCGTAAGATTGCCAATGTGACCATCGTCGCGCCGGACAg                                                                 >  1:466638/1‑250 (MQ=255)

                                                                                                                                                                                 gTTTCAATGGCAATTTGAACCGAACGGTTTGCGCCTTAAATTTTATTTGCCGGCAGGCAGTTACGCCACGACGTTGGTACGCGAGCTGGTGAATGTTGAAAACTGAAAAACGAGAAGAAAAACAGGAATAACAAGAACATGAATATTTTATTAAGTAACGATGACGGCATTCACGCGCCGGGCATTCGTGTGATgg                                                                                                                  <  1:463333/196‑1 (MQ=255)

                                                                                                                                                                                   ttCAATGGCAATTTGAACCGAACGGTTTGCGCCTTAAATTTTATTTGCCGGCAGGCAGTTACGCCACGACGTTGGTACGCGAGCTGGTg                                                                                                                                                                                                                           <  1:472100/89‑1 (MQ=255)

                                                                                                                                                                                       aTGGCAATTTGAACCGAACGGTTTGCGCCTTAAATTTTATTTGCCGGCAGGCAGTTACGCCACGACGTTGGTACGCGAGCTGGTGAATGTTGAAAACTGAAAAACGAGAAGAAAAACAGGAATAACAAGAACATGAATATTTTATTAAGTAACGATGACGGCATTCACGCGCCGGGCATTCGTGTGATGGCAGAAGCATTGCGTAAGATTGCCAATGTGACCATCGTCGCGCCGGACAGCAACCGCAgcgc                                                     <  1:891275/251‑1 (MQ=255)

                                                                                                                                                                                           cAATTTGAACCGAACGGTTTGCGCCTTAAATTTTATTTGCCGGCAGGCAGTTACGCCACGACGTTGGTACGCGAGCTGGTGAATGTTGAAAACTGAAAAACGAGAAGAAAAACAGGAATAACAAGAACATGAATATTTTATTAAGTAACGATGACGGCATTCACGCGCCGGGCATTCGTGTGATGGCAGAAGCATTGCGTAAGATTGCCAATGTGACCATCGTCGCGCCGGACAGCAACCGCAGcgccgc                                                  <  1:405012/250‑1 (MQ=255)

                                                                                                                                                                                            aaTTTGAACCGAACGGTTTGCGCCTTAAATTTTATTTGCCGGCAGGCAGTTACGCCACGACGTTGGTACGCGAGCTGGTGAATGTTGAAAACTGAAAAACGAGAAGAAAAACAGGAATAACAAGAACATGAATATTTTATTAAGTAACGATGACGGCATTCACGCGCCGGGCATTCgtg                                                                                                                        <  1:494601/179‑1 (MQ=255)

                                                                                                                                                                                            aaTTTGAACCGAACGGTTTGCGCCTTAAATTTTATTTGCCGGCAGGCAGTTACGCCACGACGTTGGTACGCGAGCTGGTGAATGTTGAAAACTGAAAAACGAGAAGAAAAACAGGAATAACAAGAACATGAATATTTTATTAAGTAACGATGACGGCATTCACGCGCCGGGCATTCGTGTGATGGCAGAAGCATTGCGTAAGATTGCCAATGTGACCATCGTCGCGCCGGACAGCAACCGCAGcgccgc                                                  >  1:311279/1‑249 (MQ=255)

                                                                                                                                                                                             aTTTGAACCGAACGGTTTGCGCCTTAAATTTTATTTGCCGGCAGGCAGTTACGCCACGACGTTGGTACGCGAGCTGGTGAATGTTGAAAACTGAAAAACGAGAAGAAAAACAGGAATAACAAGAACATGAATATTTTATTAAGTAACGATGACGGCATTCACGCGCCGGGCATTCGTGTGATGGCAGAAGCATTGCGTAAGAt                                                                                               >  1:141657/1‑203 (MQ=255)

                                                                                                                                                                                                   aCCGAACGGTTTGCGCCTTAAATTTTATTTGCCGGCAGGCAGTTACGCCACGACGTTGGTACGCGAGCTGGTGAATGTTGAAAACTGAAAAACGAGAAGAAAAACAGGAATAACAAGAACATGAATATTTTATTAAGTAACGATGACGGCATTCACGCGCCGGGCATTCGTGTGATGGCAGAAGCATTGCGTAAGATTGCCAATGTGACCATCGTCGCGCCGGACAGCAACCGCAGCGCCGCCTCCAGTTc                                         <  1:557172/251‑1 (MQ=255)

                                                                                                                                                                                                         cGGTTTGCGCCTTAAATTTTATTTGCCGGCAGGCAGTTACGCCACGACGTTGGTACGCGAGCTGGTGAATGTTGAAAACTGAAAAACGAGAAGAAAAACAGGAATAACAAGAACATGAATATTTTATTAAGTAACGATGACGGCATTCACGCGCCGGGCATTCGTGTGATGGCAGAAGCATTGCGTAAGATTGCCAATGTGACCATCGTCGCGCCGGACAGCAACCGCAGCGCCGCCTCCAGTTCCTTAAc                                   <  1:874290/251‑1 (MQ=255)

                                                                                                                                                                                                         cGGTTTGCGCCTTAAATTTTATTTGCCGGCAGGCAGTTACGCCACGACGTTGGTACGCGAGCTGGTGAATGTTGAAAACTGAAAAACGAGAAGAAAAACAGGAATAACAAGAACATGAATATTTTATTAAGTAACGATGACGGCATTCACGCGCCGGGCATTCGTGTGATGGCAGAAGCATTGCGTAAGATTGCCAATGTGACCATCGTCGCGCCGGACAGCAACCGCAGCGCCGCATCCAGTTCCTTAAc                                   <  1:139756/251‑1 (MQ=255)

                                                                                                                                                                                                          ggTTTGCGCCTTAAATTTTATTTGCCGGCAGGCAGTTACGCCACGACGTTGGTACGCGAGCTGGTGAATGTTGAAAACTGAAAAACGAGAAGAAAAACAGGAATAACAAGAACATGAATATTTTATTAAGTAACGa                                                                                                                                                     >  1:362131/1‑136 (MQ=255)

                                                                                                                                                                                                           gTTTGCGCCTTAAATTTTATTTGCCGGCAGGCAGTTACGCCACGACGTTGGTACGCGAGCTGGTGAATGTTGAAAACTGAAAAACGAGAAGAAAAACAGGAATAACAAGAACATGAATATTTTATTAAGTAACGATGACGGCATTCACGCGCCGGGCATTCGTGTGATGGCAGAAGCATTGCg                                                                                                     <  1:522997/183‑1 (MQ=255)

                                                                                                                                                                                                           gTTTGCGCCTTAAATTTTATTTGCCGGCAGGCAGTTACGCCACGACGTTGGTACGCGAGCTGGTGAATGTTGAAAACTGAAAAACGAGAAGAAAAACAGGAATAACAAGAACATGAATATTTTATTAAGTAACGATGACGGCATTCACGCGCCGGGCATTCGTGTGATGGCAGAAGCATTGCGTAAGATTGCCAATGTGACCATCGTCGCGCCGGACAGCAACCGCAGCGCCGCCTCCAGTTCCTTAAcc                                  <  1:423847/250‑1 (MQ=255)

                                                                                                                                                                                                           gTTTGCGCCTTAAATTTTATTTGCCGGCAGGCAGTTACGCCACGACGTTGGTACGCGAGCTGGTGAATGTTGAAAACTGAAAAACGAGAAGAAAAACAGGAATAACAAGAACATGAATATTTTATTAAGTAACGATGACGGCATTCACGCGCCGGGCATTCGTGTGATGGCAGAAGCATTGCGTAAGATTGCCAATGTGACCATCGTCGCGCCGGACAGCAACCGCAGCGCCGCCTCCAGTTCCTTAAcc                                  <  1:292898/250‑1 (MQ=255)

                                                                                                                                                                                                                     tAAATTTTATTTGCCGGCAGGCAGTTACGCCACGACGTTGGTACGCGAGCTGGTGAATGTTGAAAACTGAAAAACGAGAAGAAAAACAGGAATAACAAGAACATGAATATTTTATTAAGTAACGATGACGGCATTCACGCGCCGGGCATTCGTGTGATGGCAGAAGCATTGCGTAAGATTGCCAATGTGACCATCGTCGCGCCGGACAGCAACCGCAGCGCCGCCTCCAGTTCCTTAACCTTGGTGAAGcc                       <  1:64525/251‑1 (MQ=255)

                                                                                                                                                                                                                      aaaTTTTATTTGCCGGCAGGCAGTTACGCCACGACGTTGGTACGCGAGCTGGTGAATGTTGAAAACTGAAAAACGAGAAGAAAAACAGGAATAACAAGAACATGAATATTTTATTAAGTAACGATGACGGCATTCACGCGCCGGGCATTCGTGTGATGGCAGAAGCATTGCGTAAGATTGCCAATGTGACCATCGTCGCGCCGGACAGCAACCGCAGCGCCGCCTCCAGTTCCTTAAcc                                  >  1:111051/1‑239 (MQ=255)

                                                                                                                                                                                                                           ttATTTGCCGGCAGGCAGTTACGCCACGACGTTGGTACGCGAGCTGGTGAATGTTGAAAACTGAAAAACGAGAAGAAAAACAGGAATAACAAGAACATGAATATTTTATTAAGTAACGATGACGGCATTCACGCGCCGGGCATTCGTGTGATGGCAGAAGCATTGCGTAAGATTGCCAATGTGACCATCGTCGCGCCGGACAGCAACCGCAGCGCCGCCTCCAGTTCCTTAACCTTGGTGAAGCCGTTGTa                 >  1:577350/1‑251 (MQ=255)

                                                                                                                                                                                                                                  ccGGCAGGCAGTTACGCCACGACGTTGGTACGCGAGCTGGTGAATGTTGAAAACTGAAAAACGAGAAGAAAAACAGGAATAACAAGAACATGAATATTTTATTAAGTAACGATGACGGCATTCACGCGCCGGGCATTCGTGTGATGGCAGAAGCATTGCGTAAGATTGCCAATGTGACCATCGTCGCGCCGGACAGCAACCGCAGCGCCGCCTCCAGTTCCTTAACCTTGGTGAAGCCGTTGTATCCGtt           <  1:607887/250‑1 (MQ=255)

                                                                                                                                                                                                                                     gcaggcagTTACGCCACGACGTTGGTACGCGAGCTGGTGAATGTTGAAAACTGAAAAACGAGAAGAAAAACAGGAATAACAAGAACATGAATATTTTATTAAGTAACGATGACGGCATTCACGCGCCGGGCATTCGTGTGATGGCAGAAGCATTGCGTAAGATTGCCAATGTGACCATCGTCGCGCCGGACAGCAACCGCAGCGCCGCCTCCAGTTCCTTAACCTTGGTGAAGCCGTTGTATCCGTTACAt       >  1:186796/1‑251 (MQ=255)

                                                                                                                                                                                                                                          cagTTACGCCACGACGTTGGTACGCGAGCTGGTGAATGTTGAAAACTGAAAAACGAGAAGAAAAACAGGAATAACAAGAACATGAATATTTTATTAAGTAACGATGACGGCATTCACGCGCCGGGCATTCGTGTGATGGCAGAAGCATTGCGTAAGATTGCCAATGTGACCATCGTCGCGCCGGACAGCAACCGCAGCGCCGCCTCCAGTTCCTTAACCTTGGTGAAGCCGTTGTATCCGTTACATTTGGa  >  1:338365/1‑251 (MQ=255)

                                                                                                                                                                                                                                            gTTACGCCACGACGTTGGTACGCGAGCTGGTGAATGTTGAAAACTGAAAAACGAGAAGAAAAACAGGAATAACAAGAACATGAATATTTTATTAAGTAACGATGACGGCATTCACGCGCCGGGCATTCGTGTGATGGCAGAAGCATTGCGTAAGAt                                                                                               <  1:304531/156‑1 (MQ=255)

                                                                                                                                                                                                                                                  ccACGACGTTGGTACGCGAGCTGGTGAATGTTGAAAACTGAAAAACGAGAAGAAAAACAGGAATAAc                                                                                                                                                                                  >  1:66933/1‑67 (MQ=255)

GCAACAACGCTTGGCACAACGGGATATTTTGCTTACCGCACCGCTTATCGGCGAAGAGGACAAAAGTGCGGTGGATTTTGAGAATGAAATTTTTGTCGCGCACCAAGCCTTGTTCCATTTGATGCGGCAAGAACGCGTGAAAGCCGCCCGCCGTCCGATTTTAATGCAGGCGCAACAGTTTCAATGGCAATTTGAACCGAACGGTTTGCGCCTTAAATTTTATTTGCCGGCAGGCAGTTACGCCACGGCGTTGGTACGCGAGCTGGTGAATGTTGAAAACTGAAAAACGAGAAGAAAAACAGGAATAACAAGAACATGAATATTTTATTAAGTAACGATGACGGCATTCACGCGCCGGGCATTCGTGTGATGGCAGAAGCATTGCGTAAGATTGCCAATGTGACCATCGTCGCGCCGGACAGCAACCGCAGCGCCGCCTCCAGTTCCTTAACCTTGGTGAAGCCGTTGTATCCGTTACATTTGGA  >  1/533156‑533640

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`