Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	513,013	G→A	intergenic (+40/‑35)	unknown → / → unknown	Xylulose kinase (EC 2.7.1.17)/tRNA‑SeC(p)‑TCA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	513,013	0	G	A	100.0%	229.9 / NA	76	intergenic (+40/‑35)	unknown/unknown	Xylulose kinase (EC 2.7.1.17)/tRNA‑SeC(p)‑TCA
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (44/32);  total (44/32)

ATTGGGCGCCGCAATTCAAGCCATGTGGGCAAATAAACAGGGTTCGCTGGCGGAATTATGCCGAGCTTTCGTACAGTTAAATGAAGCGACGCGCACAGCGCCGATAGCGGAAAATGCGGAACAATATCAATCCGTTTACCAACGCTATCGCCAACATTTAAACGCCCAATATTCCGTATAAAATTAATGGATTATTTTTCCGCTTCACTTGAATCGCACGGGCTTTTGCGCTAAAGTTCGCATCAATTTCCAAGAGGAAGATCGTCGTTTCCGGTGAGGCGGCAGGACTTCAAATCCTGTTGAGGCTGCCAGCAGTCTCGGGTAGGTTCAACTCCTACGATCTTCCGCCAATCCATGTAATCCCGCATAAATCCACGTAAACATTCCACCAAACAAACCCAGTATTTAAAAGGCTTTATTGTAATTTATCGTAAATCCACATAAACTACTAGCAACGATTTTTAGT  >  1/512793‑513258                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                      aTTGGGCGCCGCAATTCAAGCCATGTGGGCAAATAAACAGGGTTCGCTGGCGGAATTATGCCGAGCTTTCGTACAGTTAAATGAAGCGACGCGCACAGCGCCGATAGCGGAAAATGCGGAACAATATCAATCCGTTTACCAACGCTATCGCCAACATTTAAACGCCCAATATTCCGTATAAAATTAATGGATTATTTTTCCGCTTCACTTGAATCGCACGAGCTTTTGCGCTAAAGTTCGCATCAATTTc                                                                                                                                                                                                                          >  1:780181/1‑250 (MQ=255)     ggCGCCGCAATTCAAGCCATGTGGGCAAATAAACAGGGTTCGCTGGCGGAATTATGCCGAGCTTTCGTACAGTTAAATGAAGCGACGCGCACAGCGCCGATAGCGGAAAATGCGGAACAATATCAATCCGTTTACCAACGCTATCGCCAACATTTAAACGCCCAATATTCCGTATAAAATTAATGGATTATTTTTCCGCTTCACTTGAATCGCACGAGCTTTTGCGCTAAAGTTCGCATCAATTTCCAag                                                                                                                                                                                                                      <  1:630074/250‑1 (MQ=255)            cAATTCAAGCCATGTGGGCAAATAAACAGGGTTCGCTGGCGGAATTATGCCGAGCTTTCGTACAGTTAAATGAAGCGACGCGCACAGCGCCGATAGCGGAAAATGCGGAACAATATCAATCCGTTTACCAACGCTATCGCCAACATTTAAACGCCCAATATTCCGTATAAAATTAATGGATTATTTTTCCGCTTCACTTGAATCGCACGAGCTTTTGCGCTAAAGTTCGCATCAATTTCCAAGAGGAAGAt                                                                                                                                                                                                              <  1:835093/251‑1 (MQ=255)              aTTCAAGCCATGTGGGCAAATAAACAGGGTTCGCTGGCGGAATTATGCCGAGCTTTCGTACAGTTAAATGAAGCGACGCGCACAGCGCCGATAGCGGAAAATGCGGAACAATATCAATCCGTTTACCAACGCTATCGCCAACATTTAAACGCCCAATATTCCGTATAAAATTAATGGATTATTTTTCCGCTTCACTTGAATCGCACGAGCTTTTGCGCTAAAGTTCGCATCAATTTCCAAGAGGAAg                                                                                                                                                                                                                <  1:860579/247‑1 (MQ=255)                 cAAGCCATGTGGGCAAATAAACAGGGTTCGCTGGCGGAATTATGCCGAGCTTTCGTACAGTTAAATGAAGCGACGCGCACAGCGCCGATAGCGGAAAATGCGGAACAATATCAATCCGTTTACCAACGCTATCGCCAACATTTAAACGCCCAATATTCCGTATAAAATTAATGGATTATTTTTCCGCTTCACTTGAATCGCACGAGCTTTTGCGCTAAAGTTCGCATCAATTTCCAAGAGGAAGAtcgtcg                                                                                                                                                                                                         <  1:761639/251‑1 (MQ=255)                              cAAATAAACAGGGTTCGCTGGCGGAATTATGCCGAGCTTTCGTACAGTTAAATGAAGCGACGCGCACAGCGCCGATAGCGGAAAATGCGGAACAATATCAATCCGTTTACCAACGCTATCGCCAACATTTAAACGCCCAATATTCCGTATAAAATTAATGGATTATTTTTCCGCTTCACTTGAATCGCACGAGCTTTTGCGCTAAAGTTCGCATCAATTTCCAag                                                                                                                                                                                                                      >  1:244793/1‑225 (MQ=255)                                   aaaCAGGGTTCGCTGGCGGAATTATGCCGAGCTTTCGTACAGTTAAATGAAGCGACGCGCACAGCGCCGATAGCGGAAAATGCGGAACAATATCAATCCGTTTACCAACGCTATCGCCAACATTTAAACGCCCAATATTCCGTATAAAATTAATGGATTATTTTTCCGCTTCACTTGAATCGCACGAGCTTTTGCGCTAAAg                                                                                                                                                                                                                                        <  1:634889/202‑1 (MQ=255)                                                 ggCGGAATTATGCCGAGCTTTCGTACAGTTAAATGAAGCGACGCGCACAGCGCCGATAGCGGAAAATGCGGAACAATATCAATCCGTTTACCAACGCTATCGCCAACATTTAAACGCCCAATATTCCGTATAAAATTAATGGATTATTTTTCCGCTTCACTTGAATCGCACGAGCTTTTGCGCTAAAGTTCGCATCAATTTCCAAGAGGAAGATCGTCGTTTCCGGTGAGGCGGCAGGACTTCAAATc                                                                                                                                                                            >  1:93082/1‑248 (MQ=255)                                                                  cTTTCGTACAGTTAAATGAAGCGACGCGCACAGCGCCGATAGCGGAAAATGCGGAACAATATCAATCCGTTTACCAACGCTATCGCCAACATTTAAACGCCCAATATTCCGTATAAAATTAATGGATTATTTTTCCGCTTCACTTGAATCGCACGAGCTTTTGCGCTAAAg                                                                                                                                                                                                                                        >  1:894011/1‑171 (MQ=255)                                                                    ttCGTACAGTTAAATGAAGCGACGCGCACAGCGCCGATAGCGGAAAATGCGGAACAATATCAATCCGTTTACCAACGCTATCGCCAACATTTAAACGCCCAATATTCCGTATAAAATTAATGGATTATTTTTCCGCTTCACTTGAATCGCACGAGCTTTTGCGCTAAAGTTCGCATCAATTTCCAag                                                                                                                                                                                                                      >  1:226943/1‑187 (MQ=255)                                                                    ttCGTACAGTTAAATGAAGCGACGCGCACAGCGCCGATAGCGGAAAATGCGGAACAATATCAATCCGTTTACCAACGCTATCGCCAACATTTAAACGCCCAATATTCCGTATAAAATTAATGGATTATTTTTCCGCTTCACTTGAATCGCACGAGCTTTTGCGCTAAAGTTCGCATCAATTTCCAAGAGGAAGATCGTCGTTTCCGGTGAGGCGGCAGGACTTCAAATCCTGTTGAGGCTGCCAGCAGt                                                                                                                                                        >  1:240666/1‑249 (MQ=255)                                                                            gTTAAATGAAGCGACGCGCACAGCGCCGATAGCGGAAAATGCGGAACAATATCAATCCGTTTACCAACGCTATCGCCAACATTTAAACGCCCAATATTCCGTATAAAATTAATGGATTATTTTTCCGCTTCACTTGAATCGCACGAGCTTTTGCGCTAAAGTTCGCATCAATTTCCAAGAGGAAGATCGTCGTTTCCGGTGAGGCGGCAGGACTTCAAATCCTGTTGAg                                                                                                                                                                    <  1:6931/229‑1 (MQ=255)                                                                            gTTAAATGAAGCGACGCGCACAGCGCCGATAGCGGAAAATGCGGAACAATATCAATCCGTTTACCAACGCTATCGCCAACATTTAAACGCCCAATATTCCGTATAAAATTAATGGATTATTTTTCCGCTTCACTTGAATCGCACGAGCTTTTGCGCTAAAGTTCGCATCAATTTCCAAGAGGAAGATCGTCGTTTCCGGTGAGGCGGCAGGACTTCAAATCCTGTTGAGGCTGCcagc                                                                                                                                                           <  1:33361/238‑1 (MQ=255)                                                                            gTTAAATGAAGCGACGCGCACAGCGCCGATAGCGGAAAATGCGGAACAATATCAATCCGTTTACCAACGCTATCGCCAACATTTAAACGCCCAATATTCCGTATAAAATTAATGGATTATTTTTCCGCTTCACTTGAATCGCACGAGCTTTTGCGCTAAAGTTCGCATCAATTTCCAAGAGGAAGATCGTCGTTTCCGGTGAGGCGGCAGGACTTCAAATCCTGTTGAGGCTGCCAGCAGTCTCGGGt                                                                                                                                                 >  1:529616/1‑248 (MQ=255)                                                                            gTTAAATGAAGCGACGCGCACAGCGCCGATAGCGGAAAATGCGGAACAATATCAATCCGTTTACCAACGCTATCGCCAACATTTAAACGCCCAATATTCCGTATAAAATTAATGGATTATTTTTCCGCTTCACTTGAATCGCACGAGCTTTTGCGCTAAAGTTCGCATCAATTTCCAAGAGGAAGATCGTCGTTTCCGGTGAGGCGGCAGGACTTCAAATCCTGTTGAGGCTGCCAGCAGTCTCGGGTAgg                                                                                                                                              >  1:872038/1‑251 (MQ=255)                                                                             ttAAATGAAGCGACGCGCACAGCGCCGATAGCGGAAAATGCGGAACAATATCAATCCGTTTACCAACGCTATCGCCAACATTTAAACGCCCAATATTCCGTATAAAATTAATGGATTATTTTTCCGCTTCACTTGAATCGCACGAGCTTTTGCGCTAAAGTTCGCATCAATTTCCAAGAGGAAGATCGTCGTTTCCGGTGAGGCGGCAGGACTTCAAATCCTGTTGAGGCTGCCAGCAGTCTCGGGt                                                                                                                                                 <  1:714430/247‑1 (MQ=255)                                                                                    aaGCGACGCGCACAGCGCCGATAGCGGAAAATGCGGAACAATATCAATCCGTTTACCAACGCTATCGCCAACATTTAAACGCCCAATATTCCGTATAAAATTAATGGATTATTTTTCCGCTTCACTTGAATCGCACGAGCTTTTGCGCTAAAGTTCGCATCAATTTCCAAGAGGAAGATCGTCGTTTCCGGTGAGGCGGCAGGACTTCAAATCCTGTTGAGGCTGCCAGCAGTCTCGGGTAGGTTCAACTc                                                                                                                                      <  1:288800/251‑1 (MQ=255)                                                                                        gACGCGCACAGCGCCGATAGCGGAAAATGCGGAACAATATCAATCCGTTTACCAACGCTATCGCCAACATTTAAACGCCCAATATTCCGTATAAAATTAATGGATTATTTTTCCGCTTCACTTGAATCGCACGAGCTTTTGCGCTAAAGTTCGCATCAATTTCCAAGAGAAAGATCGTCGTTTCCGGTGAGGCGGCAGGACTTCAAATCCTGTTGAGGCTGCCAGCAGTCTCGGGTAGGTTCAACTCCTa                                                                                                                                   >  1:424206/1‑250 (MQ=255)                                                                                          cgcgCACAGCGCCGATAGCGGAAAATGCGGAACAATATCAATCCGTTTACCAACGCTATCGCCAACATTTAAACGCCCAATATTCCGTATAAAATTAATGGATTATTTTTCCGCTTCACTTGAATCGCACGAGCTTTTGCGCTAAAGTTCGCATCAATTTCCAAGAGGAAGATCGTCGTTTCCGGTGAGGCGGCAGGACTTCAAATCCTGTTGAGGCTGCCAGCAGTCTCGGGTAGGTTCAACTCCTACGa                                                                                                                                <  1:398042/251‑1 (MQ=255)                                                                                          cgcgCACAGCGCCGATAGCGGAAAATGCGGAACAATATCAATCCG‑TTACCAGCGCTATCGCCAACATTTAAACGCCCAATATACCATATAGAATTAATGGATTATTTTTCCGCTTCACTTGAATCGCACGAGCTGTTGCGCTAAAGTTCGCATCAATTTCCAAGAGGAAGATCGTCGTTTCCGGTGAGGCGGCAGGACTTCAAATCCTGTTGATGCTGCCAGCAGTCTCGGGTAGGTTCAACTCCTACGAt                                                                                                                               <  1:415597/251‑1 (MQ=255)                                                                                            cgcACAGCGCCGATAGCGGAAAATGCGGAACAATATCAATCCGTTTACCAACGCTATCGCCAACATTTAAACGCCCAATATTCCGTATAAAATTAATGGATTATTTTTCCGCTTCACTTGAATCGCACGAGCTTTTGCGCTAAAGTTCGCATCAATTTCCAAGAGGAAGATCGTCGTTTCCGGTGAGGCGGCAGGACTTCAAATCCTGTTGAGGCTGCCAGCAGTCTCGGGTAGGTTCAACTCCTACGATc                                                                                                                              <  1:596085/251‑1 (MQ=255)                                                                                                   cgcCGATAGCGGAAAATGCGGAACAATATCAATCCGTTTACCAACGCTATCGCCAACATTTAAACGCCCAATATTCCGTATAAAATTAATGGATTATTTTTCCGCTTCACTTGAATCGCACGAGCTTTTGCGCTAAAGTTCGCATCAATTTCCAAGAGGAAGATCGTCGTTTCCGGTGAGGCGGCAGGACTTCAAATCCTGTTGAGGCTGCCAGCAGTCTCGGGTAGGTTCAACTCCTACGATCTTCCGCC                                                                                                                       <  1:464055‑M1/251‑1 (MQ=255)                                                                                                   cgcCGATAGCGGAAAATGCGGAACAATATCAATCCGTTTACCAACGCTATCGCCAACATTTAAACGCCCAATATTCCGTATAAAATTAATGGATTATTTTTCCGCTTCACTTGAATCGCACGAGCTTTTGCGCTAAAGTTCGCATCAATTTCCAAGAGGAAGATCGTCGTTTCCGGTGAGGCGGCAGGACTTCAAATCCTGTTGAGGCTGCCAGCAGTCTCGGGTAGGTTCAACTCCTACGATCTTCCGC                                                                                                                        <  1:752104‑M1/250‑1 (MQ=255)                                                                                                                 aaTGCGGAACAATATCAATCCGTTTACCAACGCTATCGCCAACATTTAAACGCCCAATATTCCGTATAAAATTAATGGATTATTTTTCCGCTTCACTTGAATCGCACGAGCTTTTGCGCTAAAGTTCGCATCAATTTCCAAGAGGAAGATCGTCGTTTCCGGTGAGGCGGCAGGACTTCAAATCCTGTTGAGGCTGCCAGCAGTCTCGGGTAGGTTCAACTCCTACGATCTTCCGCCActcaattttattt                                                                                                         <  1:62091‑M1/251‑14 (MQ=255)                                                                                                                  aTGCGGAACAATATCAATCCGTTTACCAACGCTATCGCCAACATTTAAACGCCCAATATTCCGTATAAAATTAATGGATTATTTTTCCGCTTCACTTGAATCGCACGAGCt                                                                                                                                                                                                                                                    >  1:524610/1‑111 (MQ=255)                                                                                                                    gCGGAACAATATCAATCCGTTTACCAACGCTATCGCCAACATTTAAACGCCCAATATTCCGTATAAAATTAATGGATTATTTTTCCGCTTCACTTGAATCGCACGAGCTTTTGCGCTAAAGTTCGCATCAATTTCCAAGAGGAAGATCGTCGTTTCCGGTGAGGCGGCAGGACTTCAAATCCTGTTGAGGCTGCCAGCAGTCTCGGGTAGGTTCAACTCCTACGATCTTCCGCCActcaattttattttta                                                                                                      >  1:664745‑M1/1‑235 (MQ=255)                                                                                                                       gAACAATATCAATCCGTTTACCAACGCTATCGCCAACATTTAAACGCCCAATATTCCGTATAAAATTAATGGATTATTTTTCCGCTTCACTTGAATCGCACGAGCTTTTGCGCTAAAg                                                                                                                                                                                                                                        >  1:312167/1‑118 (MQ=255)                                                                                                                       gAACAATATCAATCCGTTTACCAACGCTATCGCCAACATTTAAACGCCCAATATTCCGTATAAAATTAATGGATTATTTTTCCGCTTCACTTGAATCGCACGAGCTTTTGCGCTAAAGTTCGCATCAATTTCCAag                                                                                                                                                                                                                      >  1:48931/1‑136 (MQ=255)                                                                                                                       gAACAATATCAATCCGTTTACCAACGCTATCGCCAACATTTAAACGCCCAATATTCCGTATAAAATTAATGGATTATTTTTCCGCTTCACTTGAATCGCACGAGCTTTTGCGCTAAAGTTCGCATCAATTTCCAAGAGGAAGATCGTCGTTTCCGGTGAGGCGGCAGGACTTCAAATCCTGTTGAGGCTGCCAGCAGTCTCGGGTAGGTTCAACTCCTACGATCTTCCGCCActcaattttatttttaaaa                                                                                                   >  1:142991‑M1/1‑232 (MQ=255)                                                                                                                        aaCAATATCAATCCGTTTACCAACGCTATCGCCAACATTTAAACGCCCAATATTCCGTATAAAATTAATGGATTATTTTTCCGCTTCACTTGAATCGCACGAGCTTTTGCGCTAAAGTTCGCATCAATTTCCAAGAGGAAGATCGTCGTTTCCGGTGAGGCGGCAGGACTTCAAATCCTGTTGAGGCTGCCAGCAGTCTCGGGTAGGTTCAACTCCTACGATCTTCCGCCActcaattttatttttaaaaa                                                                                                  <  1:263571‑M1/251‑21 (MQ=255)                                                                                                                            atatCAATCCGTTTACCAACGCTATCGCCAACATTTAAACGCCCAATATTCCGTATAAAATTAATGGATTATTTTTCCGCTTCACTTGAATCGCACGAGCTTTTGCGCTAAAGTTCGCATCAATTTCCAAGAGGAAGATCGTCGTTTCCGGTGAGGCGGCAGGACTTCAAATCCTGTTGAGGCTGCCAGCAGTCTCGGGTAGGTTc                                                                                                                                           >  1:357923/1‑206 (MQ=255)                                                                                                                            atatCAATCCGTTTACCAACGCTATCGCCAACATTTAAACGCCCAATATTCCGTATAAAATTAATGGATTATTTTTCCGCTTCACTTGAATCGCACGAGCTTTTGCGCTAAAGTTCGCATCAATTTCCAAGAGGAAGATCGTCGTTTCCGGTGAGGCGGCAGGACTTCAAATCCTGTTGAGGCTGCCAGCAGTCTCGGGTAGGTTCAACTCCTACGATCTTCCGCCActcaattttatttttaaaaaaca                                                                                               >  1:235263‑M1/1‑227 (MQ=255)                                                                                                                             tatCAATCCGTTTACCAACGCTATCGCCAACATTTAAACGCCCAATATTCCGTATAAAATTAATGGATTATTTTTCCGCTTCACTTGAATCGCACGAGCTTTTGCGCTAAAGTTCGCATCAATTTCCAAGAGGAAGATCGTCGTTTCCGGTGAGGCGGCAGGACTTCAACTCCTGTTGAGGCTGCCAGCAGTCTCGGGTAGGTTCAACTCCTACGATCTTCCGCCActcaattttatttttaaaaaaca                                                                                               >  1:434694‑M1/1‑226 (MQ=255)                                                                                                                              atCAATCCGTTTACCAACGCTATCGCCAACATTTAAACGCCCAATATTCCGTATAAAATTAATGGATTATTTTTCCGCTTCACTTGAATCGCACGAGCTTTTGCGCTAAAGTTCGCATCAATTTCCAAGAGGAAGATCGTCGTTTCCGGTGAGGCGGCAGGACTTCAAATCCTGTTGAGGCTGCCAGCAGTCTCGGGTAGGTTCAACTCCTACGATCTTCCGCCActcaattttatttttcaaaaacactt                                                                                            >  1:793411‑M1/1‑225 (MQ=255)                                                                                                                               tCAATCCGTTTACCAACGCTATCGCCAACATTTAAACGCCCAATATTCCGTATAAAATTAATGGATTATTTTTCCGCTTCACTTGAATCGCACGAGCTTTTGCGCTAAAGTTCGCATCAATTTCCAAGAGGAAGATCGTCGTTTCCGGTGAGGCGGCAGGACTTCAAATCCTGTTGAGGCTGCCAGCAGTCTCGGGTAGGTTCAACTCCTACGATCTTCCGCCActcaattttatttttaaaaaacacttc                                                                                           <  1:66159‑M1/251‑28 (MQ=255)                                                                                                                                     cGTTTACCAACGCTATCGCCAACATTTAAACGCCCAATATTCCGTATAAAATTAATGGATTATTTTTCCGCTTCACTTGAATCGCACGAGCTTTTGCGCTAAAGTTCGCATCAATTTCCAAGAGGAAGATCGTCGTTTCCGGTGAGGCGGCAGGACTTCAAATCCTGTTGAGGCTGCCAGCAGTCTCGGGTAGGTTCAACTCCTACGATCTTCCGCCActcaattttatttttaaaaaacacttcataaaa                                                                                     <  1:729302‑M1/251‑34 (MQ=255)                                                                                                                                      gTTTACCAACGCTATCGCCAACATTTAAACGCCCAATATTCCGTATAAAATTAATGGATTATTTTTCCGCTTCACTTGAATCGCACGAGCTTTTGCGCTAAAGTTCGCATCAATTTCCAAGAGGAAGATCGTCGTTTCCGGTGAGGCGGCAGGACTTCAAATCCTGTTGAGGCTGCCAGCAGTCTCGGGTAGGTTCAACTCCTACGATCTTC                                                                                                                           <  1:699510‑M1/212‑1 (MQ=255)                                                                                                                                         tACCAACGCTATCGCCAACATTTAAACGCCCAATATTCCGTATAAAATTAATGGATTATTTTTCCGCTTCACTTGAATCGCACGAGCTTTTGCGCTAAAGTTCGCATCAATTTCCAAGAGGAAGATCGTCGTTTCCGGTGAGGCGGCAGGACTTCAAATCCTGTTGAGGCTGCCAGCAGTCTCGGGTAGGTTCAACTCCTACGATCTTCCGCCActcaattttatttttaaaaaacacttcataaaatga                                                                                  >  1:415244‑M1/1‑214 (MQ=255)                                                                                                                                         tACCAACGCTATCGCCAACATTTAAACGCCCAATATTCCGTATAAAATTAATGGATTATTTTTCCGCTTCACTTGAATCGCACGAGCTTTTGCGCTAAAGTTCGCATCAATTTCCAAGAGGAAGATCGTCGTTTCCGGTGAGGCGGCAGGACTTCAAATCCTGTTGAGGCTGCCAGCAGTCTCGGGTAGGTTCAACTCCTACGATCTTCCGCCActcaattttatttttaaaaaacacttcataaaatga                                                                                  >  1:894716‑M1/1‑214 (MQ=255)                                                                                                                                         tACCAACGCTATCGCCAACATTTAAACGCCCAATATTCCGTATAAAATTAATGGATTATTTTTCCGCTTCACTTGAATCGCACGAGCTTTTGCGCTAAAGTTCGCATCAATTTCCAAGAGGAAGATCGTCGTTTCCGGTGAGGCGGCAGGACTTCAAATCCTGTTGAGGCTGCCAGCAGTCTCGGGTAGGTTCAACTCCTACGATCTTCCGCCActcaattttatttttaaaaaacacttcataaaatga                                                                                  >  1:499458‑M1/1‑214 (MQ=255)                                                                                                                                              aCGCTATCGCCAACATTTAAACGCCCAATATTCCGTATAAAATTAATGGATTATTTTTCCGCTTCACTTGAATCGCACGAGCTTTTGCGCTAAAGTTCGCATCAATTTCCAAGAGGAAGATCGTCGTTTCCGGTGAGGCGGCAGGACTTCAAATCCTGTTGAGGCTGCCAGCAGTCTCGGGTAGGTTCAACTCCTACGATCTTCCGCCActcaattttatttttaaaaaacacttcataaaatgaccgcac                                                                            <  1:236609‑M1/251‑43 (MQ=255)                                                                                                                                                 cTATCGCCAACATTTAAACGCCCAATATTCCGTATAAAATTAATGGATTATTTTTCCGCTTCACTTGAATCGCACGAGCTTTTGCGCTAAAGTTCGCATCAATTTCCAAGAGGAAGATCGTCGTTTCCGGTGAGGCGGCAGGACTTCAAATCCTGTTGAGGCTGCCAGCAGTCTCGGGTAGGTTCAACTCCTACGATCTTCCGCCActcaattttatttttaaaaaacacttcataaaatgaccgcacttt                                                                         <  1:164962‑M1/251‑46 (MQ=255)                                                                                                                                                 cTATCGCCAACATTTAAACGCCCAATATTCCGTATAAAATTAATGGATTATTTTTCCGCTTCACTTGAATCGCACGAGCTTTTGCGCTAAAGTTCGCATCAATTTCCAAGAGGAAGATCGTCGTTTCCGGTGAGGCGGCAGGACTTCAAATCCTGTTGAGGCTGCCAGCAGTCTCGGGTAGGTTCAACTCCTACGATCTTCCGCCActcaattttatttttaaaaaacacttcataaaatgaccgcacttt                                                                         <  1:259682‑M1/251‑46 (MQ=255)                                                                                                                                                   aTCGCCAACATTTAAACGCCCAATATTCCGTATAAAATTAATGGATTATTTTTCCGCTTCACTTGAATCGCACGAGCTTTTGCGCTAAAGTTCGCATCAATTTCCAAGAGGAAGATCGTCGTTTCCGGTGAGGCGGCAGGACTTCAAATCCTGTTGAGGCTGCCAGCAGTCTCGGGTAGGTTCAACTCCTACGATCTTCCGCC                                                                                                                       >  1:399509‑M1/1‑203 (MQ=255)                                                                                                                                                       ccAACATTTAAACGCCCAATATTCCGTATAAAATTAATGGATTATTTTTCCGCTTCACTTGAATCGCACGAGCTTTTGCGCTAAAGTTCGCATCAATTTCCAAGAGGAAGATCGTCGTTTCCGGTGAGGCGGCAGGACTTCAAATCCTGTTGAGGCTGCCAGCAGTCTCGGGTAGGTTCAACTCCTACGATCTTCCGCCActcaattttatttttaaaaaacacttcataaaatgaccgcacttttgacc                                                                    <  1:563658‑M1/250‑51 (MQ=255)                                                                                                                                                          aCATTTAAACGCCCAATATTCCGTATAAAATTAATGGATTATTTTTCCGCTTCACTTGAATCGCACGAGCTTTTGCGCTAAAGTTCGCATCAATTTCCAAGAGGAAGATCGTCGTTTCCGGTGAGGCGGCAGGACTTCAAATCCTGTTGAGGCTGCCAGCAGtct                                                                                                                                                      >  1:497269/1‑165 (MQ=255)                                                                                                                                                          aCATTTAAACGCCCAATATTCCGTATAAAATTAATGGATTATTTTTCCGCTTCACTTGAATCGCACGAGCTTTTGCGCTAAAGTTCGCATCAATTTCCAAGAGGAAGATCGTCGTTTCCGGTGAGGCGGCAGGACTTCAAATCCTGTTGAGGCTGCCAGCAGTCTCGGGTAGGTTCAACTCCTACGATCTTCCGCCActcaattttatttttaaaaaacacttcataaaatgaccgcacttttgaccgaaa                                                                >  1:470098‑M1/1‑197 (MQ=255)                                                                                                                                                           cATTTAAACGCCCAATATTCCGTATAAAATTAATGGATTATTTTTCCGCTTCACTTGAATCGCACGAGCTTTTGCGCTAAAGTTCGCATCAATTTCCAAGAGGa                                                                                                                                                                                                                  >  1:301949/1‑104 (MQ=255)                                                                                                                                                           cATTTAAACGCCCAATATTCCGTATAAAATTAATGGATTATTTTTCCGCTTCACTTGAATCGCACGAGCTTTTGCGCTAAAGTTCGCATCAATTTCCAAGAGGAAGATCGTCGTTTCCGGTGAGGCGGCAGGACTTCAAATCCTGTTGAGGCTGCCAGCAGTCTCGGGt                                                                                                                                                 >  1:826831/1‑169 (MQ=255)                                                                                                                                                           cATTTAAACGCCCAATATTCCGTATAAAATTAATGGATTATTTTTCCGCTTCACTTGAATCGCACGAGCTTTTGCGCTAAAGTTCGCATCAATTTCCAAGAGGAAGATCGTCGTTTCCGGTGAGGCGGCAGGACTTCAAATCCTGTTGAGGCTGCCAGCAGTCTCGGGTAGGTTCAACTCCTACGATCTTCCGCCActcaattttatttttaaaaaacacttcataaaatgaccgcacttttgaccg                                                                   >  1:651223‑M1/1‑196 (MQ=255)                                                                                                                                                           cATTTAAACGCCCAATATTCCGTATAAAATTAATGGATTATTTTTCAGCTTCACTTGAATCGCACGAGCTTTTGCGCTAAAGTTCGCATCAATTTCCAAGAGGAAGATCGTCGTTTCCGGTGAGGCGGCAGGACTTCaaa                                                                                                                                                                              >  1:123512/1‑140 (MQ=255)                                                                                                                                                                   cGCCCAATATTCCGTATAAAATTAATGGATTATTTTTCCGCTTCACTTGAATCGCACGAGCTTTTGCGCTAAAGTTCGCATCAATTTCCAAGAGGAAGATCGTCGTTTCCGGTGAGGCGGCAGGACTTCAAATCCTGTTGAGGCTGCCAGCAGTCTCGGGTAGGTTCAACTCCTACGATCTTCCGCCActcaattttatttttaaaaaacacttcataaaatgaccgcacttttgaccgaaattgattgc                                                        >  1:642645‑M1/1‑188 (MQ=255)                                                                                                                                                                    gCCCAATATTCCGTATAAAATTAATGGATTATTTTTCCGCTTCACTTGAATCGCACGAGCTTTTGCGCTAAAGTTCGCATCAATTTCCAAGAGGAAGATCGTCGTTTCCGGTGAGGCGGCAGGACTTCAAATCCTGTTGAGGCTGCCAGCAGTCTCGGGTAGGTTCAACTCCTACGATCTTCCGCCActcaattttatttttaaaaaacacttcataaaatgaccgcacttttgaccgaaattgattgccg                                                      >  1:523930‑M1/1‑187 (MQ=255)                                                                                                                                                                    gCCCAATATTCCGTATAAAATTAATGGATTATTTTTCCGCTTCACTTGAATCGCACGAGCTTTTGCGCTAAAGTTCGCATCAATTTCCAAGAGGAAGATCGTCGTTTCCGGTGAGGCGGCAGGACTTCAAATCCTGTTGAGGCTGCCAGCAGTCTCGGGTAGGTTCAACTCCTACGATCTTCCGCCActcaattttatttttaaaaaacacttcataaaatg                                                                                   >  1:519015‑M1/1‑187 (MQ=255)                                                                                                                                                                     cccAATATTCCGTATAAAATTAATGGATTATTTTTCCGCTTCTCTTGAATCGCACGAGCTTTTGCGCTAAAGTTCGCATCAATTTCCAAGAGGAAGATCGTCGTTTCCGGTGAGGCGGCAGGACTTCAAATCCTGTTGAGGCTGCCAGCAGTCTCGGGTAGGTTCAACTCCTACGATCTTCCGCCActcaattttatttttaaaaaacacttcataaaatgaccgcacttttgaccgaaattgattgccga                                                     >  1:48180‑M1/1‑186 (MQ=255)                                                                                                                                                                     cccAATATTCCGTATAAAATTAATGGATTATTTTTCCGCTTCACTTGAATCGCACGAGCTTTTGCGCTAAAGTTCGCATCAATTTCCAAGAGGAAGAt                                                                                                                                                                                                              <  1:526937/98‑1 (MQ=255)                                                                                                                                                                     cccAATATTCCGTATAAAATTAATGGATTATTTTTCCGCTTCACTTGAATCGCACGAGCTTTTGCGCTAAAGTTCGCATCAATTTCCAAGAGGAAGATCGTCGTTTCCGGTGAGGCGGCAGGACTTCAAATCCTGTTGAGGCTGCCAGCAGTCTCGGGTAGGTTCAACTCCTACGATc                                                                                                                              <  1:896063/178‑1 (MQ=255)                                                                                                                                                                     cccAATATTCCGTATAAAATTAATGGATTATTTTTCCGCTTCACTTGAATCGCACGAGCTTTTGCGCTAAAGTTCGCATCAATTTCCAAGAGGAAGATCGTCGTTTCCGGTGAGGCGGCAGGACTTCAAATCCTGTTGAGGCTGCCAGCAGTCTCGGGTAGGTTCAACTCCTACGATCTTCCGCCActcaattttatttttaaaaaacacttcataaaatgaccgcacttttgaccgaaattgattgccga                                                     >  1:636778‑M1/1‑186 (MQ=255)                                                                                                                                                                     cccAATATTCCGTATAAAATTAATGGATTATTTTTCCGCTTCACTTGAATCGCACGAGCTTTTGCGCTAAAGTTCGCATCAATTTCCAAGAGGAAGATCGTCGTTTCCGGTGAGGCGGCAGGACTTCAAATCCTGTTGAGGCTGCCAGCAGTCTCGGGTAGGTTCAACTCCTACGATCTTCCGCCActcaattttatttttaaaaaacacttcataaaatgaccgcacttttgaccgaaattgattgccga                                                     >  1:605673‑M1/1‑186 (MQ=255)                                                                                                                                                                     cccAATATTCCGTATAAAATTAATGGATTATTTTTCCGCTTCACTTGAATCGCACGAGCTTTTGCGCTAAAGTTCGCATCAATTTCCAAGAGGAAGATCGTCGTTTCCGGTGAGGCGGCAGGACTTCAAATCCTGTTGAGGCTGCCAGCAGTCTCGGGTAGGTTCAACTCCTACGATCTTCCGCCActcaattttatttttaaaaaacacttcataaaatgaccgcacttttgaccgaaattgattgccga                                                     >  1:728588‑M1/1‑186 (MQ=255)                                                                                                                                                                     cccAATATTCCGTATAAAATTAATGGATTATTTTTCCGCTTCACTTGAATCGCACGAGCTTTTGCGCTAAAGTTCGCATCAATTTCCAAGAGGAAGATCGTCGTTTCCGGTGAGGCGGCAGGACTTCAAATCCTGTTGAGGCTGCCAGCAGTCTCGGGTAGGTTCAACTCCTACGATCTTCCGCCActcaattttattt                                                                                                         <  1:497453‑M1/199‑14 (MQ=255)                                                                                                                                                                     cccAATATTCCGTATAAAATTAATGGATTATTTTTCCGCTTCACTTGAATCGCACGAGCTTTTGCGCTAAAGTTCGCATCAATTTCCAAGAGCAAGATCGTCGTTTCCGTTGAGGCGGCAGGACTTCAAATCCTGTTGAGGCTGCCAGCAGTCTCGGGTAGGGTCAACTCCTACGACCTTCCGCCActcaattttatttttaaaaaacacttcataaaatgaccgcacttttgaccgaaattgattgccga                                                     >  1:584498‑M1/1‑186 (MQ=255)                                                                                                                                                                          tatTCCGTATAAAATTAATGGATTATTTTTCCGCTTCACTTGAATCGCACGAGCTTTTGCGCTAAAGTTCGCATCAATTTCCAAGAGGAAGATCGTCGTTTCCGGTGAGGCGGCAGGACTTCAAATCCTGTTGAGGCTGCCAGCAGTCTCGGGTAGGTTCAACTCCTACGATCTTCCGCCActcaattttatttttaaaaaacacttcataaaatgaccgcacttttgaccgaaattgattgccgactatc                                                <  1:31464‑M1/251‑71 (MQ=255)                                                                                                                                                                              ccGTATAAAATTAATGGATTATTTTTCCGCTTCACTTGAATCGCACGAGCTTTTGCGCTAAAGTTCGCATCAATTTCCAAGAGGAAGATCGTCGTTTCCGGTGAGGCGGCAGGACTTCAAATCCTGTTGAGGCTGCCAGCAGTCTCGGGTAGGTTCAACTCCTACGATCTTCCGCCActcaattttatttttaaaaaacacttcataaaatgaccgcacttttgaccgaaattgattgccgactatcagct                                            <  1:700695‑M1/251‑75 (MQ=255)                                                                                                                                                                                gTATAAAATTAATGGATTATTTTTCCGCTTCACTTGAATCGCACGAGCTTTTGCGCTAAAGTTCGCATCAATTTCCAAGAGGAAGATCGTCGTTTCCGGTGAGGCGGCAGGACTTCAAATCCTGTTGAGGCTGCCAGCAGTCTCGGGTAGGTTCAACTCCTACGa                                                                                                                                >  1:319168/1‑165 (MQ=255)                                                                                                                                                                                gTATAAAATTAATGGATTATTTTTCCGCTTCACTTGAATCGCACGAGCTTTTGCGCTAAAGTTCGCATCAATTTCCAAGAGGAAGATCGTCGTTTCCGGTGAGGCGGCAGGACTTCAAATCCTGTTGAGGCTGCCAGCAGTCTCGGGTAGGTTCAACTCCTACGATCTTCCGCCActcaattttatttttaaaaaacacttcataaaatgaccgcacttttgaccgaaattgattgccgactatcagctt                                           >  1:684963‑M1/1‑175 (MQ=255)                                                                                                                                                                                     aaaTTAATGGATTATTTTTCCGCCTCACTTGAATCGCACGAGCTTTTGCGCTAAAGTTCGCATCAATTTCCAAGAGGAAGATCGTCGTTTCCGGTGAGGCGGCAGGACTTCAAATCCTGTTGAGGCTGCCAGCAGTCTCGGGTAGGTTCAACTCCTACGATCTTCCGCCActcaattt                                                                                                              >  1:531704‑M1/1‑170 (MQ=255)                                                                                                                                                                                        ttAATGGATTATTTTTCCGCTTCACTTGAATCGCACGAGCTTTTGCGCTAAAGTTCGCATCAATTTCCAAGAGGAAGATCGTCGTTTCCGGTGAGGCGGCAGGACTTCAAATCCTGTTGAGGCTGCCAGCAGTCTCGGGTAGGTTCAACTCCTACGATCTTCCGCCActcaattttatttttaaaaaacacttcataaaatgaccgcacttttgaccgaaattgattgccgactatcagcttcggttgcag                                  <  1:753560‑M1/251‑85 (MQ=255)                                                                                                                                                                                              gATTATTTTTCCGCTTCACTTGAATCGCACGAGCTTTTGCGCTAAAGTTCGCATCAATTTCCAAGAGGAAGATCGTCGTTTCCGGTGAGGCGGCAGGACTTCAAATCCTGTTGAGGCTGCCAGCAGTCTCGGGTAGGTTCAACTCCTACGa                                                                                                                                >  1:349248/1‑151 (MQ=255)                                                                                                                                                                                                ttattTTTCCGCTTCACTTGAATCGCACGAGCTTTTGCGCTAAAGTTCGCATCAATTTCCAAGAGGAAGATCGTCGTTTCCGGTGAGGCGGCAGGACTTCAAATCCTGTTGAGGCTGCCAGCAGTCTCGGGTAGGTTCAACTCCTACGATCTTCCGCCActcaattttatttttaaaaaacacttcataaaatgaccgcacttttgaccgaaattgattgccgactatcagcttcggttgcagcgttgtca                          <  1:585517‑M1/251‑93 (MQ=255)                                                                                                                                                                                                  attTTTCCGCTTCACTTGAATCGCACGAGCTTTTGCGCTAAAGTTCGCATCAATTTCCAAGAGGAAGATCGTCGTTTCCGGTGAGGCGGCAGGACTTCaaa                                                                                                                                                                              >  1:374331/1‑101 (MQ=255)                                                                                                                                                                                                              cACTTGAATCGCACGAGCTTTTGCGCTAAAGTTCGCATCAATTTCCAAGAGGAAGATCGTCGTTTCCGGTGAGGCGGCAGGACTTCAAATCCTGTTGAGGCTGCCAGCAGTCTCGGGTAGGTTCAACTCCTACGATCTTCCGCCActcaattttatttttaaaaaacacttcataaaatgaccgcacttttgaccgaaattgattgccgactatcagcttcggttgcagcgttgtcacccgatagaagc              >  1:404451‑M1/1‑145 (MQ=255)                                                                                                                                                                                                                   gAATCGCACGAGCTTTTGCGCTAAAGTTCGCATCAATTTCCAAGAGGAAGATCGTCGTTTCCGGTGAGGCGGCAGGACTTCAAATCCTGTTGAGGCTGCCAGCAGTCTCGGGTAGGTTCAACTCCTACGATCTTCCGCCActcaattttatttttaaaaaacacttcataaaatgaccgcacttttgaccgaaattgattgccgactatcagcttcggttgcagcgttgtcacccgatagaagccatcgca       >  1:139282‑M1/1‑140 (MQ=255)                                                                                                                                                                                                                      tCGCACGAGCTTTTGCGCTAAAGTTCGCATCAATTTCCAAGAGGAAGATCGTCGTTTCCGGTGAGGCGGCAGGACTTCAAATCCTGTTGAGGCTGCCAGCAGTCTCGGGTAGGTTCAACTCCTACGATCTTCCGCCActcaattttatttttaaaaaacacttcataaaatgaccgcacttttgaccgaaattgattgccgactatcagcttcggttgcagcgttgtcacccgatagaagccatcgcatgg    <  1:42914‑M1/251‑115 (MQ=255)                                                                                                                                                                                                                       cGCACGAGCTTTTGCGTTAAAGTTCGCATCAATTTCCAAGAGGAAGATCGTCGTTTCCGGTGAGGCGGCAGGACTTCAAATCCTGTTGAGGCTGCCAGCAGTCTCGGGTAGGTTCAACTCCTACGATCTTCCGCCActcaattttatttttaaaaaacacttcataaaatgaccgcacttttgaccgaaattgattgccgactatcagcttcggttgcagcgttgtcacccgatagaagccatcgcatggt   <  1:492526‑M1/251‑116 (MQ=255)                                                                                                                                                                                                                        gCACGAGCTTTTGCGCTAAAGTTCGCATCAATTTCCAAGAGGAAGATCGTCGTTTCCGGTGAGGCGGCAGGACTTCAAATCCTGTTGAGGCTGCCAGCAGTCTCGGGTAGGTTCAACTCCTACGATCTTCCGCCActcaattttatttttaaaaaacacttcataaaatgaccgcacttttgaccgaaattgattgccgactatcagcttcggttgcagcgttgtcacccgatagaagccatcgcatggtt  >  1:712125‑M1/1‑135 (MQ=255)                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                      ATTGGGCGCCGCAATTCAAGCCATGTGGGCAAATAAACAGGGTTCGCTGGCGGAATTATGCCGAGCTTTCGTACAGTTAAATGAAGCGACGCGCACAGCGCCGATAGCGGAAAATGCGGAACAATATCAATCCGTTTACCAACGCTATCGCCAACATTTAAACGCCCAATATTCCGTATAAAATTAATGGATTATTTTTCCGCTTCACTTGAATCGCACGGGCTTTTGCGCTAAAGTTCGCATCAATTTCCAAGAGGAAGATCGTCGTTTCCGGTGAGGCGGCAGGACTTCAAATCCTGTTGAGGCTGCCAGCAGTCTCGGGTAGGTTCAACTCCTACGATCTTCCGCCAATCCATGTAATCCCGCATAAATCCACGTAAACATTCCACCAAACAAACCCAGTATTTAAAAGGCTTTATTGTAATTTATCGTAAATCCACATAAACTACTAGCAACGATTTTTAGT  >  1/512793‑513258

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑