Predicted mutation
evidence seq id position mutation annotation gene description
RA 1 506,426 A→G R292R (CGT→CGC)  unknown ← D‑xylose ABC transporter, permease protein XylH

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*1506,4260AG98.8% 227.7 / NA 82R292R (CGT→CGC) unknownD‑xylose ABC transporter, permease protein XylH
Reads supporting (aligned to +/- strand):  ref base A (1/0);  new base G (61/20);  total (62/20)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01

CATCGTATCCATCCATACGGCGAGTAATAAAATGGTACCTTTCACAATGTATTGCCAGAAGGTCGGCACGTCTAACATACTCATACCGTTGTCCAATAAGGCGATAATCAATGCACCGATAACCACGCCGTAAACGCTACCGACACCGCCGGCGAGGCTTGCACCGCCGATAACACAGGCGGCGATGGCGTCCAGTTCGGCATTTTGACCGGCGGACGGCGCGCCGGCACCTAAACGGGCACTGAGAATTAACCCGGCGATGGCGACCATTAAGCCGTTAATGGAGAAAATCATGAGTTTGATTTTTTCAACATTGATGCCTGATAATCTGGCAGCCTCAATATTACCGCCAATGGCATAAATGTGTCGTCCGAATGCGGTTTTTCGTGCAATGAACATACCGATAACCGCTAAAATCGCCAGAAGCAACACCGGGAACGGAATGCCCCGATAATCATTTAAGAGATAGATCGCACCCA  >  1/506192‑506670
                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                    
cATCGTATCCATCCATACGGCGAGTAATAAAATGGTACCTTTCACAATGTATTGCCAGAAGGTCGGCACGTCTAACATACTCATACCGTTGTCCAATAAGGCGATAATCAATGCACCGATAACCACGCCGTAAACGCTACCGACACCGCCGGCGAGGCTTGCACCGCCGATAACACAGGCGGCGATGGCGTCCAGTTCGGCATTTTGACCGGCGGACGGCGCGCCGGCACCTAAACGGGCAATGAGAATTa                                                                                                                                                                                                                                      >  1:460426/1‑251 (MQ=255)
    gTATCCATCCATACGGCGAGTAATAAAATGGTACCTTTCACAATGTATTGCCAGAAGGTCGGCACGTCTAACATACTCATACCGTTGTCCAATAAGGCGATAATCAATGCACCGATAACCACGCCGTAAACGCTACCGACACCGCCGGCGAGGCTTGCACCGCCGATAACACAGGCGGCGATGGCGTCCAGTTCGGCATTTTGACCGGCGGACGGCGCGCCGGCACCTAAGCGGGCACTGAGAATTAAcc                                                                                                                                                                                                                                   >  1:135306/1‑250 (MQ=255)
    gTATCCATCCATACGGCGAGTAATAAAATGGTACCTTTCACAATGTATTGCCAGAAGGTCGGCACGTCTAACATACTCATACCGTTGTCCAATAAGGCGATAATCAATGCACCGATAACCACGCCGTAAACGCTACCAACACCGCCGGCGAGGCTTGCACCGCCGATAACACAGGCGGCGATGGCGTCCAGTTCGGCATTTTGACCGGCGGACGGCGCGCCGGCACCTAAGCGGGCACTGAGAATTAAcc                                                                                                                                                                                                                                   >  1:26954/1‑250 (MQ=255)
         catccatACGGCGAGTAATAAAATGGTACCTTTCACAATGTATTGCCAGAAGGTCGGCACGTCTAACATACTCATACCGTTGTCCAATAAGGCGATAATCAATGCACCGATAACCACGCCGTAAACGCTACCGACACCGCCGGCGAGGCTTGCACCGCCGATAACACAGGCGGCGATGGCGTCCAGTTCGGCATTTTGACCGGCGGACGGCGCGCCGGCACCTAAGCGGGCACTGAGAATTAACCCGGCGa                                                                                                                                                                                                                             >  1:94336/1‑251 (MQ=255)
           tccatACGGCGAGTAATAAAATGGTACCTTTCACAATGTATTGCCAGAAGGTCGGCACGTCTAACATACTCATACCGTTGTCCAATAAGGCGATAATCAATGCACCGATAACCACGCCGTAAACGCTACCGACACCGCCGGCGAGGCTTGCACCGCCGATAACACAGGCGGCGATGGCGTCCAGTTCGGCATTTTGACCGGCGGACGGCGCGCCGGCACCTAAGCGGGCACTGAGAATTAACCCGGCGATg                                                                                                                                                                                                                           >  1:35975/1‑251 (MQ=255)
           tccatACGGCGAGTAATAAAATGGTACCTTTCACAATGTATTGCCAGAAGGTCGGCACGTCTAACATACTCATACCGTTGTCCAATAAGGCGATAATCAATGCACCGATAACCACGCCGTAAACGCTACCGACACCGCCGGCGAGGCTTGCACCGCCGATAACACAGGCGGCGATGGCGTCCAGTTCGGCATTTTGACCGGCGGACGGCGCGCCGGCACCTAAGCGGGCACTGAGAATTAACCCGGCGATg                                                                                                                                                                                                                           >  1:221542/1‑251 (MQ=255)
           tccatACGGCGAGTAATAAAATGGTACCTTTCACAATGTATTGCCAGAAGGTCGGCACGTCTAACATACTCATACCGTTGTCCAATAAGGCGATAATCAATGCACCGATAACCACGCCGTAAACGCTACCGACACCGCCGGCGAGGCTTGCACCGCCGATAACACAGGCGGCGATGGCGTCCAGTTCGGCATTTTGACCGGCGGACGGCGCGCCGGCACCTAAGCGGGCACTGAGAATTAACCCGGCGATg                                                                                                                                                                                                                           >  1:556748/1‑251 (MQ=255)
                                    aCCTTTCACAATGTATTGCCAGAAGGTCGGCACGTCTAACATACTCATACCGTTGTCCAATAAGGCGATAATCAATGCACCGATAACCACGCCGTAAACGCTACCGACACCGCCGGCGAGGCTTGCACCGCCGATAACACAGGCGGCGATGGCGTCCAGTTCGGCATTTTGACCGGCGGACGGCGCGCCGGCACCTAAGCGGGCACTGAGAATTAACCCGGCGATGGCGACCATTAAGCCGTTAATGgaga                                                                                                                                                                                                  >  1:386503/1‑251 (MQ=255)
                                     ccTTTCACAATGTATTGCCAGAAGGTCGGCACGTCTAACATACTCATACCGTTGTCCAATAAGGCGATAATCAATGCACCGATAACCACGCCGTAAACGCTACCGACACCGCCGGCGAGGCTTGCACCGCCGATAACACAGGCGGCGATGGCGTCCAGTTCGGCATTTTGACCGGCGGACGGCGCGCCGGCACCTAAGCGGGCACTGAGAATTAACCCGGCGATGGCGACCATTAAGCCGTTAATGGAGaa                                                                                                                                                                                                 >  1:461493/1‑251 (MQ=255)
                                      cTTTCACAATGTATTGCCAGAAGGTCGGCACGTCTAACATACTCATACCGTTGTCCAATAAGGCGATAATCAATGCACCGATAACCACGCCGTAAACGCTACCGACACCGCCGGCGAGGCTTGCACCGCCGATAACACAGGCGGCGATGGCGTCCAGTTCGGCATTTTGACCGGCGGACGGCGCGCCGGCACCTAAGCGGGCACTGAGAATTAACCCGGCGATGGCGACCATTAAGCCGTTAATGGAGaaa                                                                                                                                                                                                >  1:769366/1‑251 (MQ=255)
                                              aTGTATTGCCAGAAGGTCGGCACGTCTAACATACTCATACCGTTGTCCAATAAGGCGATAATCAATGCACCGATAACCACGCCGTAAACGCTACCGACACCGCCGGCGAGGCTTGCACCGCCGATAACACAGGCGGCGATGGCGTCCAGTTCGGCATTTTGACCGGCGGACGGCGCGCCGGCACCTAAGCGGGCACTGAGAATTAAcc                                                                                                                                                                                                                                   >  1:138554/1‑208 (MQ=255)
                                                  aTTGCCAGAAGGTCGGCACGTCTAACATACTCATACCGTTGTCCAATAAGGCGATAATCAATGCACCGATAACCACGCCGTAAACGCTACCGACACCGCCGGCGAGGCTTGCACCGCCGATAACACAGGCGGCGATGGCGTCCAGTTCGGCATTTTGACCGGCGGACGGCGCGCCGGCACCTAAGCGGGCACTGAGAATTAACCCGGCGATGGCGACCATTAAGCCGTTAATGGAGAAAATCATGAGTTTg                                                                                                                                                                                    >  1:566183/1‑251 (MQ=255)
                                                          aaGGTCGGCACGTCTAACATACTCATACCGTTGTCCAATAAGGCGATAATCAATGCACCGATAACCACGCCGTAAACGCTACCGACACCGCCGGCGAGGCTTGCACCGCCGATAACACAGGCGGCGATGGCGTCCAGTTCGGCATTTTGACCGGCGGACGGCGCGCCGGCACCTAAGCGGGCACTGAGAATTAACCCGGCGATGGCGACCATTAAGCCGTTAATGGAGAAAATCATGAGTTTGAtttttt                                                                                                                                                                             >  1:249476/1‑250 (MQ=255)
                                                            ggTCGGCACGTCTAACATACTCATACCGTTGTCCAATAAGGCGATAATCAATGCACCGATAACCACGCCGTAAACGCTACCGACACCGCCGGCGAGGCTTGCACCGCCGATAACACAGGCGGCGATGGCGTCCAGTTCGGCATTTTGACCGGCGGACGGCGCGCCGGCACCTAAGCGGGCACTGAGAATTAACCCGGCGATGGCGACCATTAAGCCGTTAATGGAGAAAATCATGAGTTTGATTTTTTCaa                                                                                                                                                                          >  1:762082/1‑251 (MQ=255)
                                                                     gTCTAACATACTCATACCGTTGTCCAATAAGGCGATAATCAATGCACCGATAACCACGCCGTAAACGCTACCGACACCGCCGGCGAGGCTTGCACCGCCGATAACACAGGCGGCGATGGCGTCCAGTTCGGCATTTTGACCGGCGGACGGCGCGCCGGCACCTAAGCGGGCACTGAGAATTAACCCGGCGATGGCGACCATTAAGc                                                                                                                                                                                                              >  1:16108/1‑206 (MQ=255)
                                                                     gTCTAACATACTCATACCGTTGTCCAATAAGGCGATAATCAATGCACCGATAACCACGCCGTAAACGCTACCGACACCGCCGGCGAGGCTTGCACCGCCGATAACACAGGCGGCGATGGCGTCCAGTTCGGCATTTTGACCGGCGGACGGCGCGCCGGCACCTAAGCGGGCACTGAGAATTAACCCGGCGATGGCGACCATTAAGCCGTTAATGGAGAAAATCATGAGTTTGATTTTTTCAACATTGATg                                                                                                                                                                  >  1:364800/1‑250 (MQ=255)
                                                                      tCTAACATACTCATACCGTTGTCCAATAAGGCGATAATCAATGCACCGATAACCACGCCGTAAACGCTACCGACACCGCCGGCGAGGCTTGCACCGCCGATAACACAGGCGGCGATGGCGTCCAGTTCGGCATTTTGACCGGCGGACGGCGCGCCGGCACCTAAGCGGGCACTGAGAATTAACCCGGCGATGGCGACCATTAAGCCGTTAATGGAGAAAATCATGAGTTTGATTTTTTCAACATTGATGc                                                                                                                                                                 >  1:781262/1‑250 (MQ=255)
                                                                         aaCATACTCATACCGTTGTCCAATAAGGCGATAATCAATGCACCGATAACCACGCCGTAAACGCTACCGACACCGCCGGCGAGGCTTGCACCGCCGATAACACAGGCGGCGATGGCGTCCAGTTCGGCATTTTGACCGGCGGACGGCGCGCCGGCACCTAAGCGGGCACTGAGAATTAACCCGGCGATGGCGACCATTAAGCCGTTAATGGAGAAAATCATGAGTTTGATTTTTTCAACATTGATGCCTGa                                                                                                                                                             >  1:783265/1‑251 (MQ=255)
                                                                         aaCATACTCATACCGTTGTCCAATAAGGCGATAATCAATGCACCGATAACCACGCCGTAAACGCTACCGACACCGCCGGCGAGGCTTGCACCGCCGATAACACAGGCGGCGATGGCGTCCAGTTCGGCATTTTGACCGGCGGACGGCGCGCCGGCACCTAAGCGGGCACTGAGAATTAACCCGGCGATGGCGACCATTAAGCCGTTAATGGAGAAAATCATGAGTTTGATTTTTTCAACATTGATGCCTGa                                                                                                                                                             >  1:835029/1‑251 (MQ=255)
                                                                             tACTCATACCGTTGTCCAATAAGGCGATAATCAATGCACCGATAACCACGCCGTAAACGCTACCGACACCGCCGGCGAGGCTTGCACCGCCGATAACACAGGCGGCGATGGCGTCCAGTTCGGCATTTTGACCGGCGGACGGCGCGCCGGCACCTAAGCGGGCACTGAGAATTAACCCGGCGATGGCGACCATTAAGCCGTTAATGGAGAAAATCATGAGTTTGATTTTTTCAACATTGATGCCTGATAAt                                                                                                                                                         >  1:547119/1‑251 (MQ=255)
                                                                             tACTCATACCGTTGTCCAATAAGGCGATAATCAATGCACCGATAACCACGCCGTAAACGCTACCGACACCGCCGGCGAGGCTTGCACCGCCGATAACACAGGCGGCGATGGCGTCCAGTTCGGCATTTTGACCGGCGGACGGCGCGCCGGCACCTAAGCGGGCACTGAGAATTAACCCGGCGATGGCGACCATTAAGCCGTTAATGGAGAAAATCATGAGTTTGATTTTTTCAACATTGATGCCTGATAAt                                                                                                                                                         >  1:41075/1‑251 (MQ=255)
                                                                               cTCATACCGTTGTCCAATAAGGCGATAATCAATGCACCGATAACCACGCCGTAAACGCTACCGACACCGCCGGCGAGGCTTGCACCGCCGATAACACAGGCGGCGATGGCGTCCAGTTCGGCATTTTGACCGGCGGACGGCGCGCCGGCACCTAAGCGGGCACTGAGAATTAACCCGGCGATGGCGACCATTAAGc                                                                                                                                                                                                              >  1:494056/1‑196 (MQ=255)
                                                                               cTCATACCGTTGTCCAATAAGGCGATAATCAATGCACCGATAACCACGCCGTAAACGCTACCGACACCGCCGGCGAGGCTTGCACCGCCGATAACACAGGCGGCGATGGCGTCCAGTTCGGCATTTTGACCGGCGGACGGCGCGCCGGCACCTAAGCGGGCACTGAGAATTAACCCGGCGATGGCGACCATTAAGCCGTTAAt                                                                                                                                                                                                       >  1:669882/1‑203 (MQ=255)
                                                                               cTCATACCGTTGTCCAATAAGGCGATAATCAATGCACCGATAACCACGCCGTAAACGCTACCGACACCGCCGGCGAGGCTTGCACCGCCGATAACACAGGCGGCGATGGCGTCCAGTTCGGCATTTTGACCGGCGGACGGCGCGCCGGCACCTAAGCGGGCACTGAGAATTAACCCGGCGATGGCGACCATTAAGCCGTTAATGGAGAAAATCATGAGTTTGATTTTTTCAACATTGATGCCTGATAAt                                                                                                                                                         >  1:798444/1‑249 (MQ=255)
                                                                                  aTACCGTTGTCCAATAAGGCGATAATCAATGCACCGATAACCACGCCGTAAACGCTACCGACACCGCCGGCGAGGCTTGCACCGCCGATAACACAGGCGGCGATGGCGTCCAGTTCGGCATTTTGACCGGCGGACGGCGCGCCGGCACCTAAGCGGGCACTGAGAATTagcc                                                                                                                                                                                                                                   >  1:438833/1‑169 (MQ=255)
                                                                                     ccGTTGTCCAATAAGGCGATAATCAATGCACCGATAACCACGCCGTAAACGCTACCGACACCGCCGGCGAGGCTTGCACCGCCGATAACACAGGCGGCGATGGCGTCCAGTTCGGCATTTTGACCGGCGGACGGCGCGCCGGCACCTAAGCGGGCACTGAGAATTAACCCGGCGATGGCGACCATTAAGCCGTTAATGGAGAAAATCATGAGTTTGATTTTTTCAACATTGATGCCTGAt                                                                                                                                                            >  1:60959/1‑240 (MQ=255)
                                                                                          gTCCAATAAGGCGATAATCAATGCACCGATAACCACGCCGTAAACGCTACCGACACCGCCGGCGAGGCTTGCACCGCCGATAACACAGGCGGCGATGGCGTCCAGTTCGGCATTTTGACCGGCGGACGGCGCGCCGGCACCTAAGCGGGCACTGAGAATTAACCCGGCGATGGCGACCATTAAGCCGTTAATGGAGAAAATCATGAGTTTGATTTTTTCAACATTGATGCCTGATAATCTGGCAGCCTCaa                                                                                                                                            >  1:73259/1‑251 (MQ=255)
                                                                                            ccAATAAGGCGATAATCAATGCACCGATAACCACGCCGTAAACGCTACCGACACCGCCGGCGAGGCTTGCACCGCCGATAACACAGGCGGCGATGGCGTCCAGTTCGGCATTTTGACCGGCGGACGGCGCGCCGGCACCTAAGCGGGCACTGAGAATTAACCCGGCGATGGCGACCATTAAGCCGTTAATGGAGAAAATCATGAGTTTGATTTTTTCAACATTGATGCCTGATAATCTGGCAGCCTCAata                                                                                                                                          >  1:477879/1‑251 (MQ=255)
                                                                                               aTAAGGCGATAATCAATGCACCGATAACCACGCCGTAAACGCTACCGACACCGCCGGCGAGGCTTGCACCGCCGATAACACAGGCGGCGATGGCGTCCAGTTCGGCATTTTGACCGGCGGACGGCGCGCCGGCACCTAAGCgg                                                                                                                                                                                                                                                   >  1:862290/1‑143 (MQ=255)
                                                                                                       aTAATCAATGCACCGATAACCACGCCGTAAACGCTACCGACACCGCCGGCGAGGCTTGCACCGCCGATAACACAGGCGGCGATGGCGTCCAGTTCGGCATTTTGACCGGCGGACGGCGCGCCGGCACCTAAGCGGGCACTGAGAATTAACCCGGCGATGGCGACCATTAAGc                                                                                                                                                                                                              >  1:792267/1‑172 (MQ=255)
                                                                                                              aTGCACCGATAACCACGCCGTAAACGCTACCGACACCGCCGGCGAGGCTTGCACCGCCGATAACACAGGCGGCGATGGCGTCCAGTTCGGCATTTTGACCGGCGGACGGCGCGCCGGCACCTAAGCGGGCACTGAGAATTAACCCGGCGATGGCGACCATTAAGCCGTTAATGGAGAAAATCATGAGTTTGATTTTTTCAACATTGATGCCTGATAATCTGGCAGCCTCAATATTACCGCCAATGGCATaa                                                                                                                        >  1:357982/1‑251 (MQ=255)
                                                                                                              aTGCACCGATAACCACGCCGTAAACGCTACCGACACCGCCGGCGAGGCTTGCACCGCCGATAACACAGGCGGCGATGGCGTCCAGTTCGGCATTTTGACCGGCGGACGGCGCGCCGGCACCTAAGCGGGCACTGAGAATTAACCCGGCGATGGCGACCATTAAGCCGTTAATGGAGAAAATCATGAGTTTGATTTTTTCAACATTGATGCCTGATAATCTGGCAGCCTCAATATTACCGCCAATGGCATaa                                                                                                                        >  1:75487/1‑251 (MQ=255)
                                                                                                                   ccGATAACCACGCCGTAAACGCTACCGACACCGCCGGCGAGGCTTGCACCGCCGATAACACAGGCGGCGATGGCGTCCAGTTCGGCATTTTGACCGGCGGACGGCGCGCCGGCACCTAAGCGGGCACTGAGAATTAACCCGGCGATGGCGACCATTAAGCCGTTAATGGAGAAAATCATGAGTTTGATTTTTTCAACAt                                                                                                                                                                       >  1:793613/1‑199 (MQ=255)
                                                                                                                   ccGATAACCACGCCGTAAACGCTACCGACACCGCCGGCGAGGCTTGCACCGCCGATAACACAGGCGGCGATGGCGTCCAGTTCGGCATTTTGACCGGCGGACGGCGCGCCGGCACCTAAGCGGGCACTGAGAATTAACCCGGCGATGGCGACCATTAAGCCGTTAATGGAGAAAATCATGAGTTTGATTTTTTCAACATTGATGCCTGATAATCTGGCAGCCTCAATATTACCGCCAATGGCATAAAtgtg                                                                                                                   >  1:657167/1‑251 (MQ=255)
                                                                                                                              attctcagtgcccgcttaggtgccgGCGAGGCTTGCACCGCCGATAACACAGGCGGCGATGGCGTCCAGTTCGGCATTTTGACCGGCGGACGGCGCGCCGGCACCTAAGCGGGCACTGAGAATTAACCCGGCGATGGCGACCATTAAGCCGTTAATGGAGAAAATCATGAGTTTGATTTTTTCAACATTGATGCCTGATAATCTGGCAGCCTCAatat                                                                                                                                         >  1:48929/22‑218 (MQ=255)
                                                                                                                                 ctcagtgcccgcttaggtgccgGCGAGGCTTGCACCGCCGATAACACAGGCGGCGATGGCGTCCAGTTCGGCATTTTGACCGGCGGACGGCGCGCCGGCACCTAAGCGGGCACTGAGAATTAACCCGGCGATGGCGACCATTAAGCCGTTAATGGAGAAAATCATGAGTTTGATTTTTTCAACATTGATGCCTGATAATCTGGCAGCCTCAATATTACCGCCAATGGCATAAATGTGTCGTCCGAATGCgg                                                                                                     >  1:748727/19‑251 (MQ=255)
                                                                                                                                        cTACCGACACCGCCGGCGAGGCTTGCACCGCCGATAACACAGGCGGCGATGGCGTCCAGTTCGGCATTTTGACCGGCGGACGGCGCGCCGGCACCTAAGCGGGCACTGAGAATTAACCCGGCGATGGCGACCATTAAGCCg                                                                                                                                                                                                            >  1:264759/1‑141 (MQ=255)
                                                                                                                                        cTACCGACACCGCCGGCGAGGCTTGCACCGCCGATAACACAGGCGGCGATGGCGTCCAGTTCGGCATTTTGACCGGCGGACGGCGCGCCGGCACCTAAGCGGGCACTGAGAATTAACCCGGCGATGGCGACCATTAAGCCGTTAATGGAGAAAATCATGAGTTTGATTTTTTCAACATTGATGCCTGATAATCTGGCAGCCTCAATATTACCGCCAATGGCATAAATGTGTCGTCCGAATGCGGTTTTTCg                                                                                              >  1:404571/1‑251 (MQ=255)
                                                                                                                                             gACACCGCCGGCGAGGCTTGCACCGCCGATAACACAGGCGGCGATGGCGTCCAGTTCGGCATTTTGACCGGCGGACGGCGCGCCGGCACCTAAGCGGGCACTGAGAATTAACCCGGCGATGGCGACCATTAAGcc                                                                                                                                                                                                             >  1:751439/1‑135 (MQ=255)
                                                                                                                                                      ggCGAGGCTTGCACCGCCGATAACACAGGCGGCGATGGCGTCCAGTTCGGCATTTTGACCGGCGGACGGCGCGCCGGCACCTAAGCGGGCACTGAGAATTAACCCGGCGATGGCGACCATTAAGCCGTTAATGGAGAAAATCATGAGTTTGATTTTTTCAACATTGATGCCTGATAATCTGGCAGCCTCAATATTAc                                                                                                                                      >  1:272125/1‑197 (MQ=255)
                                                                                                                                                      ggCGAGGCTTGCACCGCCGATAACACAGGCGGCGATGGCGTCCAGTTCGGCATTTTGACCGGCGGACGGCGCGCCGGCACCTAAGCGGGCACTGAGAATTAACCCGGCGATGGCGACCATTAAGCCGTTAATGGAGAAAATCATGAGTTTGATTTTTTCAACATTGATGCCTGATAATCTGGCAGCCTCAATATTACCGCCAATGGCATAAATGTGTCGTCCGAATGCGGTTTTTCGTGCAATGAACATa                                                                                 >  1:246749/1‑250 (MQ=255)
                                                                                                                                                       gcgggcttccccccgccgATAACACAGGCGGCGATGGCGTCCAGTTCGGCATTTTGACCGGCGGACGGCGCGCCGGCACCTAAGCGGGCACTGAGAATTAACCCGGCGATGGCGACCATTAAGCCGTTAATGGAGAAAATCATGAGTTTGATTTTTTCAACATTGATGCCTGATAATCTGGCAGCCTCAATATTACCGCCAATGGCATAAATGTGTCGTCCGAATGCGGTTTTTCGTGCAATGAACATAc                                                                                <  1:664591/238‑1 (MQ=255)
                                                                                                                                                          gggcgccccccgccgATAACACAGGCGGCGATGGCGTCCAGTTCGGCATTTTGACCGGCGGACGGCGCGCCGGCACCTAAGCGGGCACTGAGAATTAACCCGGCGATGGCGACCATTAAGCCGTTAATGGAGAAAATCATGAGTTTGATTTTTTCAACATTGATGCCTGATAATCTGGCAGCCTCAATATTACCGCCAATGGCATAAATGTGTCGTCCGAATGCGGTTTTTCGTGCAATGAACATACCGa                                                                             <  1:141676/241‑1 (MQ=255)
                                                                                                                                                           ggCTTGCACCGCCGATAACACAGGCGGCGATGGCGTCCAGTTCGGCATTTTGACCGGCGGACGGCGCGCCGGCACCTAAGCGGGCACTGAGAATTAACCCGGCGATGGCGACCATTAAGCCGTTAATGGAGAAAATCATGAGTTTGATTTTTTCAACATTGATGCCTGATAATCTGGCAGCCTCAATATTACCGCCAATGGCATAAAtg                                                                                                                     >  1:476797/1‑209 (MQ=255)
                                                                                                                                                           ggCTTGCACCGCCGATAACACAGGCGGCGATGGCGTCCAGTTCGGCATTTTGACCGGCGGACGGCGCGCCGGCACCTAAGCGGGCACTGAGAATTAACCCGGCGATGGCGACCATTAAGCCGTTAATGGAGAAAATCATGAGTTTGATTTTTTCAACATTGATGCCTGATAATCTGGCAGCCTCAATATTACCGCCAATGGCATAAATGTGTCGTCCGAATGCGGTTTTTCGTGCAATGAACATACCGATa                                                                           >  1:769153/1‑251 (MQ=255)
                                                                                                                                                             ctgcacccgccgATACCACAGGCGGCGATGGCGTCCAGTTCGGCATTTTGACCGGCGGACGGCGCGCCGGCACCTAAGCGGGCACTGAGAATTACCCCGGCGAGGGCGACCATTAAGCCGTTAATGGAGAAAATCATGAGTTTGATTTTTTCAACATTGATGCCTGATAATCTGGCAGCCTCAATATTACCGCCAATGGCATAAATGTGTCGTCCGAATGCGGTTTTTCGTGCAATGAACATACCGATAAc                                                                         <  1:351829/245‑1 (MQ=255)
                                                                                                                                                             ctccccccgccgATAACACAGGCGGCGATGGCGTCAAGTTCGGCATTTTGACCGGCGGACGGCGCGCCGGCCCCTAAGCGGGCACTGAGAATTAACCCGGCGATGGCGACCATTAAGCCGTTAATGGAGAAAATCATGAGTTTGATTTTTTCAACATTGATGCCTGATAATCGGGCAGCCTCAATATTACCGCCAATGGCATAAATGTGTCGTCCGAATGCGGTTTTTCGTGCAATGAACATACCGATAAc                                                                         <  1:536633/245‑1 (MQ=255)
                                                                                                                                                             ccccccccgccgAAAACACAGGCGGCGATGGCTTCCTGTTCGGCATTTTGACCGGGGGACGGCGCGCGGGCACCAAGGCGGGCACTGAGAATTACCCCGGCGATGGCGACCATAAAGCCGTTAATGGAAAAAATCATGAGTTTGATTTTTTCAACATTGATGCCTGATAATCGGGCAGCCTAAATATTACCGCCAATGGCATAAATGTGTCGTCCGAATGCGGTTTTTCGTGCAATGAACATACCGATAAc                                                                         <  1:181816/245‑1 (MQ=255)
                                                                                                                                                              ttGCACCGCCGATAACACAGGCGGCGATGGCGTCCAGTTCGGCATTTTGACCGGCGGACGGCGCGCCGGCACCTAAGCGGGCACTGAGAATTAACCCGGCGATGGCGACCATTAAGCCGTTAATGGAGAAAATCATGAGTTTGATTTTTTCAACATTGATGCCTGATAATCTGGCAGCCTCAATATTACCGCCAATGGCATAAATGTGTCGTCCGAATGCGGTTTTTCGTGCAATGAACATACCGATAAc                                                                         >  1:340596/1‑250 (MQ=255)
                                                                                                                                                                     gccgATAACACAGGCGGCGATGGCGTCCAGTTCGGCATTTTGACCGGCGGACGGCGCGCCGGCACCTAAGCGGGCACTGAGAATTAACCCGGCGATGGCGACCATTAAGCCGTTAATGGAGAAAATCATGAGTTTGATTTTTTCAACATTGATGCCTGATAATCTGGCAGCCTCAATATTACCGCCAATGGCATAAATGTGTCGTCCGAATGCGGTTTTTCGTGCAATGAACATACCGATAACCGCTaaaa                                                                 >  1:890996/1‑251 (MQ=255)
                                                                                                                                                                     gccgATAACACAGGCGGCGATGGCGTCCAGTTCGGCATTTTGACCGGCGGACGGCGCGCCGGCACCTAAGCGGGCACTGAGAATTAACCCGGCGATGGCGACCATTAAGCCGTTAATGGAGAAAATCATGAGTTTGATTTTTTCAACATTGATGCCTGATAATCTGGCAGCCTCAATATTACCGCCAATGGCATAAATGTGTCGTCCGAATGCGGTTTTTCGTGCAATGAACATACCGATAACCGCTaaaa                                                                 >  1:140547/1‑251 (MQ=255)
                                                                                                                                                                      ccgATAACACGGGCGGCGATGGCGTCCAGTTCGGCATTTTGACCGGCGGACGGCGCGCCGGCACCTAAGCGGGCACTGAGAATTACCCCGGCGATGGCGACCATTAAGCCGTTAATGGAGAAAATCATGAGTTTGATTTTTTCAACATTGATGCCTGATAATCTGGCAGCCTCAATATTACCGCCAATGGCATAAATGTGTCGTCCGAATGCGGt                                                                                                    <  1:11122/215‑1 (MQ=255)
                                                                                                                                                                      ccgATAACACAGGCGGCGATGGCGTCCAGTTCGGCATTTTGACCGGCGGACGGCGCGCCGGCACCTAAGCGGGCACTGAGAATTAACCCGGCGATGGCGACCATTAAGCCGTTAATGGAGAAAATCATGAGTTTGATTTTTTCAACATTGATGCCTGATAATCTGGCAGCCTCAATATTACCGCCAATGGCATAAATGTGTCGTCCGAATGCGGTTTTTCGTGCAATGAACATACCGATAACCGCTAAAAt                                                                >  1:249717/1‑251 (MQ=255)
                                                                                                                                                                       cgATAACACAGGCGGCGATGGCGTCCAGTTCGGCATTTTGACCGGCGGACGGCGCGCCGGCACCTAAGCGGGCACTGAGAATTAACCCGGCGATGGCGACCATTAAGCCGTTAATGGAGAAAATCATGAGTTTGATTTTTTCAACATTGATGCCTGATAATCTGGc                                                                                                                                                    <  1:257753/166‑1 (MQ=255)
                                                                                                                                                                       cgATAACACAGGCGGCGATGGCGTCCAGTTCGGCATTTTGACCGGCGGACGGCGCGCCGGCACCTAAGCGGGCACTGAGAATTAACCCGGCGATGGCGACCATTAAGCCGTTAATGGAGAAAATCATGAGTTTGATTTTTTCAACATTGATGCCTGATAATCTGGCAGCCTCAATATTACCGCCAATGGCATAAATGTGTCGTCCGAATGCGGTTTTTCGTGCAATGAACATACCGATAACCGCTAAAAt                                                                >  1:627544/1‑250 (MQ=255)
                                                                                                                                                                          tAACACAGGCGGCGATGGCGTCCAGTTCGGCATTTTGACCGGCGGACGGCGCGCCGGCACCTAAGCGGGCACTGAGAATTAACCCGGCGATGGCGACCATTAAGCCGTTAATGGAGAAAATCATGAGTTTGATTTTTTCAACATTGATGCCTGATAATCTGGCAGCCTCAATATTACCGCCAATGGCATAAATGTGTCGTCCGAATGCGGTTTTTCGTGCAATGAACATACCGATAACCGCTAAAATCGc                                                             >  1:639193/1‑250 (MQ=255)
                                                                                                                                                                                 ggcggcGATGGCGTCCAGTTCGGCATTTTGACCGGCGGACGGCGCGCCGGCACCTAAGCGGGCACTGAGAATTAACCCGGCGATGGCGACCATTAAGc                                                                                                                                                                                                              >  1:558039/1‑98 (MQ=255)
                                                                                                                                                                                   cggcgATGGCGTCCAGTTCGGCATTTTGACCGGCGGACGGCGCGCCGGCACCTAAGCGGGCACTGAGAATTAACCCGGCGATGGCGACCATTAAGCCGTTAATGGAGAAAATCATGAGTTTGATTTTTTCAACATTGATGCCTGATAATCTGGCAGCCTCAATATTACCGCCAATGGCATAAATGTGTCGTCCGAATGCGGTTTTTCGTGCAATGAACATACCGATAACCGCTAAAATCGCCAGAAGCaa                                                    >  1:162953/1‑250 (MQ=255)
                                                                                                                                                                                   cggcgATGGCGTCCAGTTCGGCATTTTGACCGGCGGACGGCGCGCCGGCACCTAAGCGGGCACTGAGAATTAACCCGGCGATGGCGACCATTAAGCCGTTAATGGAGAAAATCATGAGTTTGATTTTTTCAACATTGATGCCTGATAATCTGGCAGCCTCAATATTACCGCCAATGGCATAAATGTGTCGTCCGAATGCGGTTTTTCGTGCAATGAACATACCGATAACCGCTAAAATCGCCAGAAGCaa                                                    >  1:338488/1‑250 (MQ=255)
                                                                                                                                                                                   cggcgATGGCGTCCAGTTCGGCATTTTGACCGGCGGACGGCGCGCCGGCACCTAAGCGGGCACTGAGAATTAACCCGGCGATGGCGACCATTAAGCCGTTAATGGAGAAAATCATGAGTTTGATTTTTTCAACATTGATGCCTGATAATCTGGCAGCCTCAATATTACCGCCAATGGCATAAATGTGTCGTCCGAATGCGGTTTTTCGTGCAATGAACATACCGATAACCGCTAAAATCGCCAGAAGCAac                                                   <  1:587823/251‑1 (MQ=255)
                                                                                                                                                                                    ggcgATGGCGTCCAGTTCGGCATTTTGCCCGGCGGACGGCGCGCCGGCACCTAGGCGGGCACTGAGAATTAACCCGGCGATGGCGACCATTAAGCCGTTAATGGAGAAAATCATGAGTTTGATTTTTTCAACATTGATGCCTGATAATCTGGCAGCCTCAATATTACCGCCAATGGCATAAAtg                                                                                                                     <  1:50482/184‑1 (MQ=255)
                                                                                                                                                                                      cggtGGCGTCCGGTCCGGCATTTTGCCCGGCGGACGGCGCGCCGGCCCCAAAGCGGGCACTGAGAATTACCCCGGCGATGGCGACCATTAAGCCGTTAATGGAGAAAATCATGAGTTTGATTTTTTCAACATTGATGCTTGATAATCGGGCGGCTTAATTGTTGCCGCCAATGGCATAAATGTGTCGTCCGAATGCGGTTTTTCGTGCAATGAACATACCGATAACCGCTAAAATCGCCAGAAGCAacac                                                 <  1:51772/247‑1 (MQ=255)
                                                                                                                                                                                            cGTCCAGTTCGGCATTTTGACCGGCGGACGGCGCGCCGGCCCCTAAGCGGGCACTGAGAATTAACCCGGCGATGGCGACCATTAAGCCGTTAATGGAGAAAATCATGAGTTTGATTTTTTCAACATTGATGCCTGATAATCTGGCAGCCTAAATATTACCGCCAATGGCATAAATGTGTCGTCCGAATGCGGTTTTTCGTGCAATGAACATACCGATAACCGCTAAAATCGCCAGAAGCAACACCGGGaa                                           <  1:200104/250‑1 (MQ=255)
                                                                                                                                                                                             gTCCAGTTCGGCATTTTGACCGGCGGACGGCGCGCCGGCACCTAAGCGGGCACTGAGAATTAACCCGGCGATGGCGACCATTAAGCCGTTAATGGAGAAAATCATGAGTTTGATTTTTTCAACATTGATGCCTGATAATCTGGCAGCCTCAATATTACCGCCAATGGCATAAAtg                                                                                                                     >  1:378521/1‑175 (MQ=255)
                                                                                                                                                                                             gTCCAGTTCGGCATTTTGACCGGCGGACGGCGCGCCGGCACCTAAGCGGGCACTGAGAATTAACCCGGCGATGGCGACCATTAAGCCGTTAATGGAGAAAATCATGAGTTTGATTTTTTCAACATTGATGCCTGATAATCTGGCAGCCTCAATATTACCGCCAATGGCATAAATGTGTCGTCCGAATGCGGTTTTTCGTGCAATGAACATACCGATAACCGCTAAAATCGCCAGAAGCAACACCggg                                             >  1:446304/1‑247 (MQ=255)
                                                                                                                                                                                             gTCCAGTTCGGCATTTTGACCGGCGGACGGCGCGCCGGCACCTAAGCGGGCACTGAGAATTAACCCGGCGATGGCGACCATTAAGCCGTTAATGGAGAAAATCATGAGTTTGATTTTTTCAACATTGATGCCTGATAATCTGGCAGCCTCAATATTACCGCCAATGGCATAAATGTGTCGTCCGAATGCGGTTTTTCGTGCAATGAACATACCGATAACCGCTAAAATCGCCAGAAGCAACACCGGGAACg                                         >  1:515308/1‑251 (MQ=255)
                                                                                                                                                                                                  gTTCGGCATTTTGACCGGCGGACGGCGCGCCGTCACCTAAGCGGGCACTGAGAATTAACCCGGCGATGGCGACCATTAAGCCGTTAATGGAGAAAATCATGAGTTTGATTTTTTCAACATTGATGCCTGACAATCTGGCAGCCTCAATATTACCGCCAATGGCATAAATGTGTCGTCCGAATGCGGTTTTTCGTGCAATGAACATACCGATAACCGCTAAAATCGCCAGAAGCAACACCGGGAACGGAATg                                    >  1:475329/1‑251 (MQ=255)
                                                                                                                                                                                                   ttCGGCATTTTGACCGGCGGACGGCGCGCCGGCACCTAAGCGGGCACTGAGAATTAACCCGGCGATGGCGACCATTAAGCCGTTAATGGAGAAAATCATGAGTTTGATTTTTTCAACATTGATGCCTGATAATCTGGCAGCCTCAATATTACCGCCAATGGCATAAATGTGTCGTCCGAATGCGGTTTTTCGTGCAATGAACATACCGATAACCGCTAAAATCGCCAGAAGCAACACCGGGAACGGAAt                                     <  1:42330/249‑1 (MQ=255)
                                                                                                                                                                                                       gCATTTTGACCGGCGGACGGCGCGCCGGCACCTAAGCGGGCACTGAGAATTAACCCGGCGATGGCGACCATTAAGCCGTTAATGGAGAAAATCATGAGTTTGATTTTTTCAACATTGATGCCTGATAATCTGGCAGCCTCAATATTACCGCCAATGGCATAAAtg                                                                                                                     <  1:261051/165‑1 (MQ=255)
                                                                                                                                                                                                       gCATTTTGACCGGCGGACGGCGCGCCGGCACCTAAGCGGGCACTGAGAATTAACCCGGCGATGGCGACCATTAAGCCGTTAATGGAGAAAATCATGAGTTTGATTTTTTCAACATTGATGCCTGATAATCTGGCAGCCTCAATATTACCGCCAATGGCATAAATGT‑‑‑GTCCGAATGCGGTTTTTCGTGCAATGAACATACCGATAACCGCTAAAATCGCCAGAAGCAACACCGGGAACGGAATGCCCCGATa                            >  1:24118/1‑251 (MQ=255)
                                                                                                                                                                                                         aTTTTGACCGGCGGACGGCGCGCCGGCACCTAAGCGGGCACTGAGAATTAACCCGGCGATGGCGACCATTAAGCCGTTAATGGAGAAAATCATGAGTTTGATTTTTTCAACATTGATGCCTGATAATCTGGc                                                                                                                                                    <  1:260244/132‑1 (MQ=255)
                                                                                                                                                                                                              gcccGGCGGGCGGCGGGCCGGCACATAAGCGGGCGCTAAGAATTAACCGGGCGATGGCGACCATTAAGCCTTTAATGGAAAAAATCATGAGTTTGATTTTTTCAACATTGATGCCTGATAATCGGGCAGCCTAAATATTACCGCCAATGGCATAGATGTGTCGTCCGAATGCGGTTTTTCGTGCAATGAACATACCGATAACCGCTAAAATCGCCAGAAGCAACACCGGGAACGGAATGCCCCGATAATc                         <  1:452642/248‑1 (MQ=255)
                                                                                                                                                                                                               cccGGCGGACGGCGCGCCGGCCCCTAAGCGGGCACTGAAAATTAACCCGGCGATGGCGACCATTAAGCCGTAAATGGAAAAAATCATGGGTTTAATTTTTTCAACATTGATGCCTAAAAATCTGACAGCCAAAATATTACCGCCAATGGCATAAATGTGTCGTCCGAATGCGGTTTTTCGTGCAATGAACATACCGATAACCGCTAAAATCGCCAGAAGCAACACCGGGAACGGAATGCCCCGATAATCAt                       <  1:230017/250‑1 (MQ=255)
                                                                                                                                                                                                                     ggccGGCGCGCGGGCACCTAAGGGGGCACTGAAAATTACCCCGGCGATGGCGACCATAAAGCCGTTAAGGGAGAAAATCATGAGTTTGATTTTTTCAACATTGATGCCGGATAATCTGGCAGCCTCAATATTACCGCCAATGGCATAAATGTGTCGTCCGAATGCGGTTTTTCGTGCAATGAACATACCGATAACCGCTAAAATCGCCAGAAGCAACACCGGGAACGGAATGCCCCGATAATCATTTAag                  <  1:870402/247‑1 (MQ=255)
                                                                                                                                                                                                                             cgcCGGCACCTAAGGGGGCACTGAGAATTAACCCGGCGATGGCGACCATTAAGCCGTTAATGGAGAAAATCATGAGTTTGATTTTTTCAACATTGATGCCTGATAATCGGGCAGCCTAAATATTACCGCCAATGGCATAAATGTGTCGTCCGAATGCGGTTTTTCGTGCAATGAACATACCGATAACCGCTAAAATCGCCAGAAGCAACACCGGGAACGGAATGCCCCGATAATCATTTAAGagatagat          <  1:825082/250‑1 (MQ=255)
                                                                                                                                                                                                                             cgcCGGCACCTAAGCGGGCACTGAGAATTACCCCGGCGATGGCGACCATTAAGCCGTTAATGGAGAAAATCATGAGTTTGATTTTTTCAACATTGATGCCTGATAATCTGGCAGCCTCAATATTACCGCCAATGGCATAAATGTGTCGTCCGAATGCGGTTTTTCGTGCAATGAACATACCGATAACCGCTAAAATCGCCAGAAGCAACACCGGGAACGGAATGCCCCGATAATCATTTAAGagatagat          <  1:440527/250‑1 (MQ=255)
                                                                                                                                                                                                                                  gCACCTAAGCGGGCACTGAGAATTAACCCGGCGATGGCGACCATTAAGCCGTTAATGGAGAAAATCATGAGTTTGATTTTTTCAACATTGATGCCTGATAATCTGGCAGCCTCAATATTACCGCCAATGGCATAAATGTGTCGTCCGAATGCGGTTTTTCGTGCAATGAACATACCGATAACCGCTAAAATCGCCAGAAGCAACACCGGGAACGGAATGCCCCGATAATCATTTAAGAGATAGATCGCAc     >  1:476937/1‑250 (MQ=255)
                                                                                                                                                                                                                                   cACCTAAGCGGGCACTGAGAATTACCCCGGCGATGGCGACAATTAAGCCGTTAATGGAGAAAATCATGAGTTTGATTTTTTCAACATTGATGCCTGATAATCTGGCAGCCTCAATATTACCGCCAATGGCATAAATGTGTCGTCCGAATGCGGTTTTTCGTGCAATGAACATACCGATAACCGCTAAAATCGCCAGAAGCAACACCGGGAACGGAATGCCCCGATAATCATTTAAGAGATAGATCGCAccc   <  1:647445/251‑1 (MQ=255)
                                                                                                                                                                                                                                    aCCTAAGCGGGCACTGAGAATTAACCCGGCGATGGCGACCATTAAGCCGTTAATGGAGAAAATCATGAGTTTGATTTTTTCAACATTGATGCCTGATAATCTGGCAGCCTCAATATTACCGCCAATGGCATAAATGTGTCGTCCGAATGCGGTTTTTCGTGCAATGAACATACCGATAACCGCTAAAATCGCCAGAAGCAACACCGGGAACGGAATGCCCCGATAATCATTTAAGagatagat          >  1:336906/1‑243 (MQ=255)
                                                                                                                                                                                                                                    aCCTAAGCGGGCACTGAGAATTAACCCGGCGATGGCGACCATTAAGCCGTTAATGGAGAAAATCATGAGTTTGATTTTTTCAACATTGATGCCTGATAATCTGGCAGCCTCAATATTACCGCCAATGGCATAAATGTGTCGTCCGAATGCGGTTTTTCGTGCAATGAACATACCGATAACCGCTAAAATCGCCAGAAGCAACACCGGGAACGGAATGCCCCGATAATCATTTAAGAGATAGATCGCACCCa  >  1:335830/1‑251 (MQ=255)
                                                                                                                                                                                                                                     ccTAAGCGGGCACTGAGAATTAACCCGGCGATGGCGACCATTAAGCCGTTAATGGAGAAAATCATGAGTTTGATTTTTTCAACATTGATGCCTGATAATCTGGCAGCCTCAATATTACCGCCAATGGCATAAATGTGTCGTCCGAATGCGGTTTTTCGTGCAATGAACATACCGATAACCGCTAAAATCGCCAGAAGCAACACCGGGAACGGAATGCCCCGATAATCATTTAag                  >  1:727004/1‑234 (MQ=255)
                                                                                                                                                                                                                                     ccTAAGCGGGCACTGAGAATTAACCCGGCGATGGCGACCATTAAGCCGTTAATGGAGAAAACCATGAGTTTGATTTTTTCAACATTGATGCCTGATAATCTGGCAGCCTCAATAtt                                                                                                                                        >  1:888952/1‑116 (MQ=255)
                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                    
CATCGTATCCATCCATACGGCGAGTAATAAAATGGTACCTTTCACAATGTATTGCCAGAAGGTCGGCACGTCTAACATACTCATACCGTTGTCCAATAAGGCGATAATCAATGCACCGATAACCACGCCGTAAACGCTACCGACACCGCCGGCGAGGCTTGCACCGCCGATAACACAGGCGGCGATGGCGTCCAGTTCGGCATTTTGACCGGCGGACGGCGCGCCGGCACCTAAACGGGCACTGAGAATTAACCCGGCGATGGCGACCATTAAGCCGTTAATGGAGAAAATCATGAGTTTGATTTTTTCAACATTGATGCCTGATAATCTGGCAGCCTCAATATTACCGCCAATGGCATAAATGTGTCGTCCGAATGCGGTTTTTCGTGCAATGAACATACCGATAACCGCTAAAATCGCCAGAAGCAACACCGGGAACGGAATGCCCCGATAATCATTTAAGAGATAGATCGCACCCA  >  1/506192‑506670

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑