Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	482,738	T→C	S37S (TCT→TCC)	unknown →	ATP synthase F0 sector subunit a (EC 3.6.3.14)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	482,738	0	T	C	100.0%	223.7 / NA	76	S37S (TCT→TCC)	unknown	ATP synthase F0 sector subunit a (EC 3.6.3.14)
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (47/29);  total (47/29)

GTAACATATTTTATTGTTTTCTTTACAGGTTTTTTTACAGCATTAATGCTAAATAATATTATCCCATTTCTGCTGAATAGGTCTTAAGTTTTATTTTACAAAGGGTTAACTATGTCTGAACAAACAACTTCAGAATATATCGGTCACCACTTATCTTTCCTTAAAACGGGGGACGGTTTCTGGAATATTCACGTTGATACCTTATTTTTCTCTATTCTGTCTGCCGTTATTTTCCTTTTCATTTTCAGACGGGTTGCTAAAAAGGCAACGCCGGGTGTACCGGGAAAATTACAATGTCTGGTGGAAATCCTGGTAGGTTGGGTTAACGGCATCGTTAAAGAAAATTTCCATGGTCCGCGCGAGGTGGTGGCACCGATTGCCCTGACCATTTTCTGTTGGGTATTCATTATGAATGCCATCGACTTAATCCCTGTTGATTTCCTTCCGCAACTTGCCGGATTATTC  >  1/482517‑482981                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                    gTAACATATTTTATTGTTTTCTTTACAGGTTTTTTTACAGCATTAATGCTAAATAATATTATCCCATTTCTGCTGAATAGGTCTTAAGTTTTATTTTACAAAGGGTTAACTATGTCTGAACAAACAACTTCAGAATATATCGGTCACCACTTATCTTTCCTTAAAACGGGGGACGGTTTCTGGAATATTCACGTTGATACCTTATTTTTCTCTATTCTGTCCGCCGTTATTTTCCTTTTCATTTTCAGACg                                                                                                                                                                                                                        >  1:496062/1‑251 (MQ=255)                                        gCATTAATGCTAAATAATATTATCCCATTTCTGCTGAATAGGTCTTAAGTTTTATTTTACAAAGGGTTAACTATGTCTGAACAAACAACTTCAGAATATATCGGTCACCACTTATCTTTCCTTAAAACGGGGGACGGTTTCTGGAATATTCACGTTGATACCTTATTTTTCTCTATTCTGTCCGCCGTTATTTTCCTTTTCATTTTCAGACGGGTTGCTAAAAAGGCAACGCCGGGTGTACCGGGAAAAtt                                                                                                                                                                                 >  1:357565/1‑251 (MQ=255)                                            tAATGCTAAATAATATTATCCCATTTCTGCTGAATAGGTCTTAAGTTTTATTTTACAAAGGGTTAACTATGTCTGAACAAACAACTTCAGAATATATCGGTCACCACTTATCTTTCCTTAAAACGGGGGACGGTTTCTGGAATATTCACGTTGATACCTTATTTTTCTCTATTCTGTCCGCCGTTATTTTCCTTTTCATTTTCAGACGGGTTGCTAAAAAGGCAACGCCGGGTGTACCGGGAAAATTACaa                                                                                                                                                                             <  1:149806/251‑1 (MQ=255)                                                   aaaTAATATTATCCCATTTCTGCTGAATAGGTCTTAAGTTTTATTTTACAAAGGGTTAACTATGTCTGAACAAACAACTTCAGAATATATCGGTCACCACTTATCTTTCCTTAAAACGGGGGACGGTTTCTGGAATATTCACGTTGATACCTTATTTTTCTCTATTCTGTCCGCCGTTATTTTCCTTTTCATTTTCAGACGGGTTGCTAAAAAGGCAACGCCGGGTGTAcc                                                                                                                                                                                          >  1:40151/1‑231 (MQ=255)                                                   aaaTAATATTATCCCATTTCTGCTGAATAGGTCTTAAGTTTTATTTTACAAAGGGTTAACTATGTCTGAACAAACAACTTCAGAATATATCGGTCACCACTTATCTTTCCTTAAAACGGGGGACGGTTTCTGGAATATTCACGTTGATACCTTATTTTTCTCTATTCTGTCCGCCGTTATTTTCCTTTTCATTTTCAGACGGGTTGCTAAAAAGGCAACGCCGGGTGTACCGGGAAAATTACAATGTCtgg                                                                                                                                                                      >  1:820455/1‑251 (MQ=255)                                                      taataTTATCCCATTTCTGCTGAATAGGTCTTAAGTTTTATTTTACAAAGGGTTAACTATGTCTGAACAAACAACTTCAGAATATATCGGTCACCACTTATCTTTCCTTAAAACGGGGGACGGTTTCTGGAATATTCACGTTGATACCTTATTTTTCTCTATTCTGTCCGCCGTTATTTTCCTTTTCATTTTCAGACGGGTTGCTAAAAAGGCAACGCCGGGTGTACCGGGAAAATTACAATGTCtggtgg                                                                                                                                                                   <  1:266343/251‑1 (MQ=255)                                                        atatTATCCCATTTCTGCTGAATAGGTCTTAAGTTTTATTTTACAAAGGGTTAACTATGTCTGAACAAACAACTTCAGAATATATCGGTCACCACTTATCTTTCCTTAAAACGGGGGACGGTTTCTGGAATATTCACGTTGATACCTTATTTTTCTCTATTCTGTCCGCCGTTATTTTCCTTTTCATTTTCAGACGGGTTGCTAAAAAGGCAACGCCGGGTGTACCGGGAAAATTACAATGTCTGGTGGaa                                                                                                                                                                 >  1:290365/1‑251 (MQ=255)                                                          attatCCCATTTCTGCTGAATAGGTCTTAAGTTTTATTTTACAAAGGGTTAACTATGTCTGAACAAACAACTTCAGAATATATCGGTCACCACTTATCTTTCCTTAAAACGGGGGACGGTTTCTGGAATATTCACGTTGATACCTTATTTTTCTCTATTCTGTCCGCCGTTATTTTCCTTTTCATTTTCAGACGGGTTGCTAAAAAGGCAACGCCGGGTGTACCGGGAAAATTACAATGTCTGGTGGAAAt                                                                                                                                                               >  1:301434/1‑251 (MQ=255)                                                            tatCCCATTTCTGCTGAATAGGTCTTAAGTTTTATTTTACAAAGGGTTAACTATGTCTGAACAAACAACTTCAGAATATATCGGTCACCACTTATCTTTCCTTAAAACGGGGGACGGTTTCTGGAATATTCACGTTGATACCTTATTTTTCTCTATTCTGTCCGCCGTTATTTTCCTTTTCATTTTCAGACGGGTTGCTAAAAAGGCAACGCCGGGTGTACCGGGAAAATTACAATGTCTGGTGGAAATcc                                                                                                                                                             <  1:156911/251‑1 (MQ=255)                                                                      ctgctgAATAGGTCTTAAGTTTTATTTTACAAAGGGTTAACTATGTCTGAACAAACAACTTCAGAATATATCGGTCACCACTTATCTTTCCTTAAAACGGGGGACGGTTTCTGGAATATTCACGTTGATACCTTATTTTTCTCTATTCTGTCCGCCGTTATTTTCCTTTTCATTTTCAGACGGGTTGCTAAAAAGGCAACGCCGGGTGTACCGGGAAAATTACAATGTCTGGTGGAAATCCTGGt                                                                                                                                                         >  1:581429/1‑245 (MQ=255)                                                                           gAATAGGTCTTAAGTTTTATTTTACAAAGGGTTAACTATGTCTGAACAAACACCTTCAGAATATATCGGTCACCACTTATCTTTCCTTAAAACGGGGGACGGTTTCTGGAATATTCACGTTGATACCTTATTTTTCTCTATTCTGTCCGCCGTTATTTTCCTTTTCATTTTCAGACGGGTTGCTAAAAAGGCAACGCCGGGTGTACCGGGAAAATTAc                                                                                                                                                                               <  1:343148/218‑1 (MQ=255)                                                                              tAGGTCTTAAGTTTTATTTTACAAAGGGTTAACTATGTCTGAACAAACAACTTCAGAATATATCGGTCACCACTTATCTTTCCTTAAAACGGGGGACGGTTTCTGGAATATTCACGTTGATACCTTATTTTTCTCTATTCTGTCCGCCGTTATTTTCCTTTTCATTTTCAGACGGGTTGCTAAAAAGGCAACGCCGGGTGTACCGGGAAAATTACAATGTCTGGTGGAAATCCTGGTAGGTTGGGTTAACg                                                                                                                                           <  1:743563/251‑1 (MQ=255)                                                                                ggTCTTAAGTTTTATTTTACAAAGGGTTAACTATGTCTGAACAAACAACTTCAGAATATATCGGTCACCACTTATCTTTCCTTAAAACGGGGGACGGTTTCTGGAATATTCACGTTGATACCTTATTTTTCTCTATTCTGTCCGCCGTTATTTTCCTTTTCATTTTCAGACGGGTTGCTAAAAAGGCAACGCCGGGTGTACCGGGAAAATTACAATGTCTGGTGGAAATCCTGGTAGGTTGGGTTAACgg                                                                                                                                          >  1:260808/1‑250 (MQ=255)                                                                                ggTCTTAAGTTTTATTTTACAAAGGGTTAACTATGTCTGAACAAACAACTTCAGAATATATCGGTCACCACTTATCTTTCCTTAAAACGGGGGACGGTTTCTGGAATATTCACGTTGATACCTTATTTTTCTCTATTCTGTCCGCCGTTATTTTCCTTTTCATTTTCAGACGGGTTGCTAAAAAGGCAACGCCGGGTGTACCGGGAAAATTACAATGTCTGGTGGAAATCCTGGTAGGTTGGGTTAACgg                                                                                                                                          >  1:717734/1‑250 (MQ=255)                                                                                 gTCTTAAGTTTTATTTTACAAAGGGTTAACTATGTCTGAACAAACAACTTCAGAATATATCGGTCACCACTTATCTTTCCTTAAAACGGGGGACGGTTTCTGGAATATTCACGTTGATACCTTATTTTTCTCTATTCTGTCCGCCGTTATTTTCCTTTTCATTTTCAGACGGGTTGCTAAAAAGGCAACGCCGGGTGTACCGGGAAAATTAc                                                                                                                                                                               >  1:569774/1‑212 (MQ=255)                                                                                 gTCTTAAGTTTTATTTTACAAAGGGTTAACTATGTCTGAACAAACAACTTCAGAATATATCGGTCACCACTTATCTTTCCTTAAAACGGGGGACGGTTTCTGGAATATTCACGTTGATACCTTATTTTTCTCTATTCTGTCCGCCGTTATTTTCCTTTTCATTTTCAGACGGGTTGCTAAAAAGGCAACGCCGGGTGTACCGGGAAAATTACAATGTCTGGTGGAAATCCTGGTAGGTTGGGTTAACGGCa                                                                                                                                        >  1:474211/1‑251 (MQ=255)                                                                                           ttattttaCAAAGGGTTAACTATGTCTGAACAAACAACTTCAGAATATATCGGTCACCACTTATCTTTCCTTAAAACGGGGGACGGTTTCTGGAATATTCACGTTGATACCTTATTTTTCTCTATTCTGTCCGCCGTTATTTTCCTTTTCATTTTCAGACGGGTTGCTAAAAAGGCAACGCCGGGTGTACCGGGAAAATTACAATGTCTGGTGGAAATCCTGGTAGGTTGGGTTAACGGCATCGTTAAAGa                                                                                                                              >  1:320049/1‑251 (MQ=255)                                                                                                ttaCAAAGGGTTAACTATGTCTGAACAAACAACTTCAGAATATATCGGTCACCCCTTATCTTTCCTTAAAACGGGGGACGGCTTCTGGAATATTCACGTTGTTACCTTATTTTTCTCTATTCTGTCCGCCGTTATTTTCTTTTTCATTTTCAGACGGGTTGCTAAAAAGGCAACGCCGGGTGTACCGGGAAAATTACAATGTCTGGTGGAAATCCTGGTAGGTTGGGTTAACGGCATCGTAAAAGAAAAtt                                                                                                                         >  1:53715/1‑251 (MQ=255)                                                                                                       gggTTAACTATGTCTGAACAAACAACTTCAGAATATATCGGTCACCACTTATCTTTCCTTAAAACGGGGGACGGTTTCTGGAATATTCACGTTGATACCTTATTTTTCTCTATTCTGTCCGCCGTTATTTTCCTTTTCATTTTCAGACGGGTTGCTAAAAAGGCAACGCCGGGTGTACCGGGAAAATTACAATGt                                                                                                                                                                          >  1:418014/1‑195 (MQ=255)                                                                                                       gggTTAACTATGTCTGAACAAACAACTTCAGAATATATCGGTCACCACTTATCTTTCCTTAAAACGGGGGACGGTTTCTGGAATATTCACGTTGATACCTTATTTTTCTCTATTCTGTCCGCCGTTATTTTCCTTTTCATTTTCAGACGGGTTGCTAAAAAGGCAACGCCGGGTGTACCGGGAAAATTACAATGTCTGGTGGAAATCCTGGTAGGTTGGGTTAACGGCATCGTTAAAGAAAATTTCCATgg                                                                                                                  >  1:43800/1‑251 (MQ=255)                                                                                                       gggTTAACTATGTCTGAACAAACAACTTCAGAATATATCGGTCACCACTTATCTTTCCTTAAAACGGGGGACGGTTTCTGGAATATTCACGTTGATACCTTATTTTTCTCTATTCTGTCCGCCGTTATTTTCCTTTTCATTTTCAGACGGGTTGCTAAAAAGGCAACGCCGGGTGTACCGGGAAAATTACAATGTCTGGTGGAAATCCTGGTAGGTTGGGTTAACGGCATCGTTAAAGAAAATTTCCATgg                                                                                                                  >  1:899375/1‑251 (MQ=255)                                                                                                       gggTTAACTATGTCTGAACAAACAACTTCAGAATATATCGGTCACCACTTATCTTTCCTTAAAACGGGGGACGGTTTCTGGAATATTCACGTTGATACCTTATTTTTCTCTATTCTGTCCGCCGTTATTTTCCTTTTCATTTTCAGACGGGTTGCTAAAAAGGCAACGCCGGGTGTACCGGGAAAATTACAATGTCTGGTGGAAATCCTGGTAGGTTGGGTTAACGGCATCGTTAAAGAAAATTTCCATgg                                                                                                                  >  1:705708/1‑251 (MQ=255)                                                                                                       gggTTAACTATGTCTGAACAAACAACTTCAGAATATATCGGTCACCACTTATCTTTCCTTAAAACGGGGGACGGTTTCTGGAATATTCACGTTGATACCTTATTTTTCTCTATTCTGTCCGCCGTTATTTTCCTTTTCATTTTCAGACGGGTTGCTAAAAAGGCAACGCCGGGTGTACCGGGAAAATTACAATGTCTGGTGGAAATCCTGGTAGGTTGGGTTAACGGCATCGTTAAAGAAAATTTCCATgg                                                                                                                  >  1:704845/1‑251 (MQ=255)                                                                                                         tttAACTATGTCTGAACAAACAACTTCAGAATATATCGGTCACCACTTATCTTTCCTTAAAACGGGGGACGGTTTCTGGAATATTCACGTTGATACCTTATTTTTCTCTATTCTGTCCGCCGTTATTTTCCTTTTCATTTTCAGACGGGTTGCTAAAAAGGCAACGCCGGGTGTACCGGGAAAATTACAATGTCTGGTGGAAATCCTGGTAGGTTGGGTTAACGGCATCGTTAAAGAAAATTTCCATGGTc                                                                                                                <  1:214069/250‑1 (MQ=255)                                                                                                            aaCTATGTCTGAACAAACAACTTCAGAATATATCGGTCACCACTTATCTTTCCTTAAAACGGGGGACGGTTTCTGGAATATTCACGTTGATACCTTATTTTTCTCTATTCTGTCCGCCGTTATTTTCCTTTTCATTTTCAGACGGGTTGCTAAAAAGGCAACGCCGGGTGTACCGGGAAAATTACAATGTCTGGTGGAAATCCTGGTAGGTTGGGTTAACGGc                                                                                                                                         <  1:743756/223‑1 (MQ=255)                                                                                                            aaCTATGTCTGAACAAACAACTTCAGAATATATCGGTCACCACTTATCTTTCCTTAAAACGGGGGACGGTTTCTGGAATATTCACGTTGATACCTTATTTTTCTCTATTCTGTCCGCCGTTATTTTCCTTTTCATTTTCAGACGGGTTGCTAAAAAGGCAACGCCGGGTGTACCGGGAAAATTACAATGTCTGGTGGAAATCCTGGTAGGTTGGGTTAACGGCATCGTTAAAGAAAATTTCCATGGTCcg                                                                                                              >  1:521503/1‑250 (MQ=255)                                                                                                            aaCTATGTCTGAACAAACAACTTCAGAATATATCGGTCACCACTTATCTTTCCTTAAAACGGGGGACGGTTTCTGGAATATTCACGTTGATACCTTATTTTTCTCTATTCTGTCCGCCGTTATTTTCCTTTTCATTTTCAGACGGGTTGCTAAAAAGGCAACGCCGGGTGTACCGGGAAAATTACAATGTCTGGTGGAAATCCTGGTAGGTTGGGTTAACGGCATCGTTAAAGAAAATTTCCATGGTCcg                                                                                                              >  1:419433/1‑250 (MQ=255)                                                                                                            aaCTATGTCTGAACAAACAACTTCAGAATATATCGGTCACCACTTATCTTTCCTTAAAACGGGGGACGGTTTCTGGAATATTCACGTTGATACCTTATTTTTCTCTATTCTGTCCGCCGTTATTTTCCTTTTCATTTTCAGACGGGTTGCTAAAAAGGCAACGCCGGGTGTACCGGGAAAATTACAATGTCTGGTGGAAATCCTGGTAGGTTGGGTTAACGGCATCGTTAAAGAAAATTTCCATGGTCcg                                                                                                              >  1:588267/1‑250 (MQ=255)                                                                                                            aaCTATGTCTGAACAAACAACTTCAGAATATATCGGTCACCACTTATCTTTCCTTAAAACGGGGGACGGTTTCTGGAATATTCACGTTGATACCTTATTTTTCTCTATTCTGTCCGCCGTTATTTTCCTTTTCATTTTCAGACGGGTTGCTAAAAAGGCAACGCCGGGTGTACCGGGAAAATTACAATGTCTGGTGGAAATCCTGGTAGGTTGGGTTAACGGCATCGTTAAAGAAAATTTCCATGGTCcg                                                                                                              >  1:641207/1‑250 (MQ=255)                                                                                                                aTGTCTGAACAAACAACTTCAGAATATATCGGTCACCACTTATCTTTCCTTAAAACGGGGGACGGTTTCTGGAATATTCACGTTGATACCTTATTTTTCTCTATTCTGTCCGCCGTTATTTTCCTTTTCATTTTCAGACGGGTTGCTAAAAAGGCAACGCCGGGTGTACCGGGAAAATTACAATGTCTGGTGGAAATCCTGGTAGGTTGGGTTAACGGCATCGTTAAAGAAAATTTCCATGGTCCGCGCGa                                                                                                         <  1:341153/251‑1 (MQ=255)                                                                                                                           aacaacTTCAGAATATATCGGTCACCACTTATCTTTCCTTAAAACGGGGGACGGTTTCTGGAATATTCACGTTGATACCTTATTTTTCTCTATTCTGTCCGCCGTTATTTTCCTTTTCATTTTCAGACGGGTTGCTAAAAAGGCAACGCCGGGTGTACCGGGAAAATTACAATGTCTGGTGGAAATCCTGGTAGGTTGGGTTAACGGCATCGTTAAAGAAAATTTCCATGGTCCGCGCGAGGTGGTGGCAc                                                                                              <  1:6942/251‑1 (MQ=255)                                                                                                                           aacaacTTCAGAATATATCGGTCACCACTTATCTTTCCTTAAAACGGGGGACGGTTTCTGGAATATTCACGTTGATACCTTATTTTTCTCTATTCTGTCCGCCGTTATTTTCCTTTTCATTTTCAGACGGGTTGCTAAAAAGGCAACGCCGGGTGTACCGGGAAAATTACAATGTCTGGTGGAAATCCTGGTAGGTTGGGTTAACGGCATCGTTAAAGAAAATTTCCATGGTCCGCGCGAGGTGGTGGCAc                                                                                              <  1:461387/251‑1 (MQ=255)                                                                                                                             caacTTCAGAATATATCGGTCACCACTTATCTTTCCTTAAAACGGGGGACGGTTTCTGGAATATTCACGTTGATACCTTATTTTTCTCTATTCTGTCCGCCGTTATTTTCCTTTTCATTTTAAGACGGGTTGCTAAAAAGGCAACGCCGGGTGTACCGGGAAAATTACAATGTCTGGTGGAAATCCTGGTAGGTTGGGTTAACGCCATCGTTAAAGAAAATTTCCATGGTCCGCGCGAGGTGGTGGCACCg                                                                                            >  1:609361/1‑251 (MQ=255)                                                                                                                                cTTCAGAATATATCGGTCACCACTTATCTTTCCTTAAAACGGGGGACGGTTTCTGGAATATTCACGTTGATACCTTATTTTTCTCTATTCTGTCCGCCGTTATTTTCCTTTTCATTTTCAGACGGGTTGCTAAAAAGGCAACGCCGGGTGTAcc                                                                                                                                                                                          >  1:430592/1‑154 (MQ=255)                                                                                                                                cTTCAGAATATATCGGTCACCACTTATCTTTCCTTAAAACGGGGGACGGTTTCTGGAATATTCACGTTGATACCTTATTTTTCTCTATTCTGTCCGCCGTTATTTTCCTTTTCATTTTCAGACGGGTTGCTAAAAAGGCAACGCCGGGTGTACCGGGAAAATTACAATGTCTGGTGGAAATCCTGGTAGGTTGGGTTAACGGCATCGTTAAAGAAAATTTCCATGGTCCGCGCGAGGTGGTGGCACCGAtt                                                                                         >  1:651229/1‑251 (MQ=255)                                                                                                                                cTTCAGAATATATCGGTCACCACTTATCTTTCCTTAAAACGGGGGACGGTTTCTGGAATATTCACGTTGATACCTTATTTTTCTCTATTCTGTCCGCCGTTATTTTCCTTTTCATTTTCAGACGGGTTGCTAAAAAGGCAACGCCGGGTGTACCGGGAAAATTACAATGTCTGGTGGAAATCCTGGTAGGTTGGGTTAACGGCATCGTTAAAGAAAATTTCCATGGTCCGCGCGAGGTGGTGGCACCGAtt                                                                                         >  1:535272/1‑251 (MQ=255)                                                                                                                                     gAATATATCGGTCACCACTTATCTTTCCTTAAAACGGGGGACGGTTTCTGGAATATTCACGTTGATACCTTATTTTTCTCTATTCTGTCCGCCGTTATTTTCCTTTTCATTTTCAGACGGGTTGCTAAAAAGGCAACGCCGGGTGTACCGGGAAAATTACAATGTCTGGTGGAAATCCTGGTAGGTTGGGTTAACgg                                                                                                                                          <  1:96069/197‑1 (MQ=255)                                                                                                                                     gAATATATCGGTCACCACTTATCTTTCCTTAAAACGGGGGACGGTTTCTGGAATATTCACGTTGATACCTTATTTTTCTCTATTCTGTCCGCCGTTATTTTCCTTTTCATTTTCAGACGGGTTGCTAAAAAGGCAACGCCGGGTGTACCGGGAAAATTACAATGTCTGGTGGAAATCCTGGTAGGTTGGGTTAACGGCATCGTTAAAGAAAATTTCCATGGTCCGCGCGAGGTGGTGGc                                                                                                >  1:702991/1‑239 (MQ=255)                                                                                                                                     gAATATATCGGTCACCACTTATCTTTCCTTAAAACGGGGGACGGTTTCTGGAATATTCACGTTGATACCTTATTTTTCTCTATTCTGTCCGCCGTTATTTTCCTTTTCATTTTCAGACGGGTTGCTAAAAAGGCAACGCCGGGTGTACCGGGAAAATTACAATGTCTGGTGGAAATCCTGGTAGGTTGGGTTAACGGCATCGTTAAAGAAAATTTCCATGGTCCGCGCGAGGTGGTGGCACCGATTGcc                                                                                      >  1:189518/1‑249 (MQ=255)                                                                                                                                         atatCGGTCACCACTTATCTTTCCTTAAAACGGGGGACGGTTTCTGGAATATTCACGTTGATACCTTATTTTTCTCTATTCTGTCCGCCGTTATTTTCCTTTTCATTTTCAGACGGGTTGCTAAAAAGGCAACGCCGGGTGTACCGGGAAAATTACAATGTCTGGTGGAAATCCTGGTAGGTTGGGTTAACGGCATCGTTAAAGAAAATTTCCATGGTCCGCGCGAGGTGGTGGCACCGATTGCCCTGAc                                                                                 >  1:673154/1‑250 (MQ=255)                                                                                                                                                tCACCACTTATCTTTCCTTAAAACGGGGGACGGTTTCTGGAATATTCACGTTGATACCTTATTTTTCTCTATTCTGTCCGCCGTTATTTTCCTTTTCATTTTCAGACGGGTTGCTAAAAAGGCAACGCCGGGTGTACCGGGAAAATTACAATGTCTGGTGGAAATCCTGGTAGGTTGGGTTAACGGCATCGTTAAAGAAAATTTCCATGGTCCGCGCGAGGTGGTGGCACCGATTGCCCTGACCATTTTc                                                                          <  1:587169/250‑1 (MQ=255)                                                                                                                                                  cccacTTATCTTTCCTTAAAACGGGGGACGGTTTCTGGAATATTCACGTTGATACCTTATTTTTCTCTATTCTGTCCGCCGTTATTTTCCTTTTCATTTTCAGACGGGTTGCTAAAAAGGCAACGCCGGGTGTACCGGGAAAATTACAATGTCTGGTGGAAATCCTGGTAGGTTGGGTTAACGGCATCGTTAAAGAAAATTTCCATGGTCCGCGCGAGGTGGTGGCACCGATTGCCCTGACCATTTTCTGt                                                                       <  1:279776/250‑1 (MQ=255)                                                                                                                                                   ccacTTATCTTTCCTTAAAACGGGGGACGGTTTCTGGAATATTCACGTTGATACCTTATTTTTCTCTATTCTGTCCGCCGTTATTTTCCTTTTCATTTTCAGACGGGTTGCTaa                                                                                                                                                                                                               <  1:94124/114‑1 (MQ=255)                                                                                                                                                   ccacTTATCTTTCCTTAAAACGGGGGACGGTTTCTGGAATATTCACGTTGATACCTTATTTTTCTCTATTCTGTCCGCCGTTATTTTCCTTTTCATTTTCAGACGGGTTGCTAAAAAGGCAACGCCGGGTGTACCGGGAAAATTACAATGTCTGGTGGAAATCCTGGTAGGTTGGGTTAAc                                                                                                                                            <  1:626939/181‑1 (MQ=255)                                                                                                                                                   ccacTTATCTTTCCTTAAAACGGGGGACGGTTTCTGGAATATTCACGTTGATACCTTATTTTTCTCTATTCTGTCCGCCGTTATTTTCCTTTTCATTTTCAGACGGGTTGCTAAAAAGGCAACGCCGGGTGTACCGGGAAAATTACAATGTCTGGTGGAAATCCTGGTAGGTTGGGTTAACGGCATCGTTAAAGAAAATTTCCATGGTCcgcgcg                                                                                                          >  1:534157/1‑215 (MQ=255)                                                                                                                                                   ccacTTATCTTTCCTTAAAACGGGGGACGGTTTCTGGAATATTCACGTTGATACCTTATTTTTCTCTATTCTGTCCGCCGTTATTTTCCTTTTCATTTTCAGACGGGTTGCTAAAAAGGCAACGCCGGGTGTACCGGGAAAATTACAATGTCTGGTGGAAATCCTGGTAGGTTGGGTTAACGGCATCGTTAAAGAAAATTTCCATGGTCCGCGCGAGGTGGTGGCACCGATTGCCCTGACCATTTTCTGtt                                                                      >  1:31560/1‑251 (MQ=255)                                                                                                                                                      cTTATCTTTCCTTAAAACGGGGGACGGTTTCTGGAATATTCACGTTGATACCTTATTTTTCTCTATTCTGTCCGCCGTTATTTTCCTTTTCATTTTCAGACGGGTTGCTAAAAAGGCAACGCCGGGTGTACCGGGAAAATTACAATGTCTGGTGGAAATCCTGGTAGGTTGGGTTAACGGCATCGTTAAAGAAAATTTCCATGGTCCGCGCGAGGTGGTGGCACCGATTGCCCTGACCATTTTc                                                                          >  1:717098/1‑244 (MQ=255)                                                                                                                                                          tCTTTCCTTAAAACGGGGGACGGTTTCTGGAATATTCACGTTGATACCTTATTTTTCTCTATTCTGTCCGCCGTTATTTTCCTTTTCATTTTCAGACGGGTTGCTAAAAAGGCAACGCCGGgtg                                                                                                                                                                                              <  1:540847/124‑1 (MQ=255)                                                                                                                                                               ccTTAAAACGGGGGACGGTTTCTGGAATATTCACGTTGATACCTTATTTTTCTCTATTCTGTCCGCCGTTATTTTCCTTTTCATTTTCAGACGGGTTGCTAAAAAGGCAACGCCGGGTGTACCGGGAAAATTACAATGTCTGGTGGAAATCCTGGTAGGTTGGGTTAACGGCATCGTTAAAGAAAATTTCCATgg                                                                                                                  >  1:771270/1‑195 (MQ=255)                                                                                                                                                                      aCGGGGGACGGTTTCTGGAATATTCACGTTGATACCTTATTTTTCTCTATTCTGTCCGCCGTTATTTTCCTTTTCATTTTCAGACGGGTTGCTAAAAAGGCAACGCCGGGTGTACCGGGAAAATTACaa                                                                                                                                                                             <  1:131431/129‑1 (MQ=255)                                                                                                                                                                              cGGTTTCTGGAATATTCACGTTGATACCTTATTTTTCTCTATTCTGTCCGCCGTTATTTTCCTTTTCATTTTCAGACGGGTTGCTAAAAAGGCAACGCCGGGTGTACCGGGAAAATTACAATGTCTGGTGGAAATCCTGGTAGGTTGGGTTAACGGCATCGTTAAAGAAAATTTCCATGGTCCGCGCGAGGTGGTGGCACCGATTGCGCTGACCATTTTc                                                                          >  1:96832/1‑220 (MQ=255)                                                                                                                                                                                  ttCTGGAATATTCACGTTGATACCTTATTTTTCTCTATTCTGTCCGCCGTTATTTTCCTTTTCATTTTCAGACGGGTTGCTAAAAAGGCAACGCCGGGTGTACCGGGAAAATTACAATGTCTGGTGGAAATCCTGGTAGGTTGGGTTAACGGCATCGTTAAAGAAAATTTCCATGGTCCGCGCGAGGTGGTGGCGCCGATTGCCCTGACCATTTTc                                                                          <  1:890000/216‑1 (MQ=255)                                                                                                                                                                                   tCTGGAATATTCACGTTGATACCTTATTTTTCTCTATTCTGTCCGCCGTTATTTTCCTTTTCATTTTCAGACGGGTTGCTAAAAAGGCAACGCCGGGTGTACCGGGAAAATTACAATGTCTGGTGGAAATCCTGGTAGGTTGGGTTAACGGCATCGTTAAAGAAAATTTCCATGGTCCGCGCGAGGTGGTGGCACCGATTGCCCTGACCATTTTCTGTTGGGTATTCATTATGAATGCCATCGACTTAATc                                      <  1:370549/251‑1 (MQ=255)                                                                                                                                                                                    cTGGAATATTCACGTTGATACCTTATTTTTCTCTATTCTGTCCGCCGTTATTTTCCTTTTCATTTTCAGACGGGTTGCTAAAAAGGCAACGCCGGGTGTACCGGGAAAATTACAATGTCTGGTGGAAATCCTGGTAGGTTGGGTTAACGGCATCGTTAAAGAAAATTTCCATGGTCCGCGCGAGGTGGTGGCACCGATTGCCCTGACCATTTTCTGTTGGGTATTCATTATGAATGCCATCGACTTAATcc                                     <  1:428047/251‑1 (MQ=255)                                                                                                                                                                                    cTGGAATATTCACGTTGATACCTTATTTTTCTCTATTCTGTCCGCCGTTATTTTCCTTTTCATTTTCAGACGGGTTGCTAAAAAGGCAACGCCGGGTGTACCGGGAAAATTACAATGTCTGGTGGAAATCCTGGTAGGTTGGGTTAACGGCATCGTTAAAGAAAATTTCCATGGTCCGCGCGAGGTGGTGGCACCGATTGCCCTGACCATTTTCTGTTGGGTATTCATTATGAATGCCATCGACTTAATcc                                     <  1:297290/251‑1 (MQ=255)                                                                                                                                                                                           atTCACGTTGATACCTTATTTTTCTCTATTCTGTCCGCCGTTATTTTCCTTTTCATTTTCAGACGGGTTGCTAAAAAGGCAACGCCGGgtg                                                                                                                                                                                              <  1:176640/91‑1 (MQ=255)                                                                                                                                                                                           atTCACGTTGATACCTTATTTTTCTCTATTCTGTCCGCCGTTATTTTCCTTTTCATTTTCAGACGGGTTGCTAAAAAGGCAACGCCGGGTGTACCGGGAAAATTACAATGTCTGGTGGAAATCCTGGTAGGTTGGGTTAACGGCATCGTTAAAGAAAATTTCCATGGTCCGCGCGAGGTGGTGGCACCGATTGCCCTGACCATTTTCTGTTGGGTATTCATTATGAATGCCATCGACTTAATCCCTGTTGa                              >  1:428483/1‑251 (MQ=255)                                                                                                                                                                                                cGTTGATACCTTATTTTTCTCTATTCTGTCCGCCGTTATTTTCCTTTTCATTTTCAGACGGGTTGCTAAAAAGGCAACGCCGGGTGTACCGGGAAAATTACAATGTCTGGTGGAAATCCTGGTAGGTTGGGTTAACGGCATCGTTAAAGAAAATTTCCATGGTc                                                                                                                >  1:258928/1‑164 (MQ=255)                                                                                                                                                                                                       aCCTTATTTTTCTCTATTCTGTCCGCCGTTATTTTCCTTTTCATTTTCAGACGGGTTGCTAAAAAGGCAACGCCGGGTGTACCGGGAAAATTACAATGTCTGGTGGAAATCCTGGTAGGTTGGGTTAACGGCATCGTTAAAGAAAATTTCCATGGTCCGCGCGAGGTGGTGGCACCGATTGccc                                                                                     <  1:851278/184‑1 (MQ=255)                                                                                                                                                                                                            aTTTTTCTCTATTCTGTCCGCCGTTATTTTCCTTTTCATTTTCAGACGGGTTGCTAAAAAGGCAACGCCGGGTGTACCGGGAAAATTACAATGTCTGGTGGAAATCCTGGTAGGTTGGGTTAACGGCATCGTTAAAGAAAATTTCCATGGTCCGCGCGAGGTGGTGGCACCGATTGCCCTGACCATTTTCTGTTGGGTATTCAt                                                            >  1:829362/1‑204 (MQ=255)                                                                                                                                                                                                            aTTTTTCTCTATTCTGTCCGCCGTTATTTTCCTTTTCATTTTCAGACGGGTTGCTAAAAAGGCAACGCCGGGTGTACCGGGAAAATTACAATGTCTGGTGGAAATCCTGGTAGGTTGGGTTAACGGCATCGTTAAAGAAAATTTCCATGGTCCGCGCGAGGTGGTGGCACCGATTGCCCTGACCATTTTCTGTTGGGTATTCATTATGAATGCCATCGACTTAATCCCTGTTGATTTCCTTCCGCAACTTg             >  1:114065/1‑251 (MQ=255)                                                                                                                                                                                                            aTTTTTCTCTATTCTGTCCGCCGTTATTTTCCTTTTCATTTTCAGACGGGTTGCTAAAAAGGCAACGCCGGGTGTACCGGGAAAATTACAATGTCTGGTGGAAATCCTGGTAGGTTGGGTTAACGGCATCGTTAAAGAAAATTTCCATGGTCCGCGCGAGGTGGTGGCACCGATTGCCCTGACCATTTTCTGTTGGGTATTCATTATGAATGCCATCGACTTAATCCCTGTTGATTTCCTTCCGCAACTTg             >  1:850496/1‑251 (MQ=255)                                                                                                                                                                                                                 tctcTATTCTGTCCGCCGTTATTTTCCTTTTCATTTTCAGACGGGTTGCTAAAAAGGCAACGCCGGGTGTACCGGGAAAATTACAATGTCTGGTGGAAATCCTGGTAGGTTGGGTTAACGGCATCGTTAAAGAAAATTTCCATGGTCCGCGCGAGGTGGTGGCACCGATTGCCCTGACCATTTTCTGTTGGGTATTCAtt                                                           <  1:354114/200‑1 (MQ=255)                                                                                                                                                                                                                 tctcTATTCTGTCCGCCGTTATTTTCCTTTTCATTTTCAGACGGGTTGCTAAAAAGGCAACGCCGGGTGTACCGGGAAAATTACAATGTCTGGTGGAAATCCTGGTAGGTTGGGTTAACGGCATCGTTAAAGAAAATTTCCATGGTCCGCGCGAGGTGGTGGCACCGATTGCCCTGACCATTTTCTGTTGGGTATTCATTATGAATGCCATCGACTTAATc                                      <  1:800699/221‑1 (MQ=255)                                                                                                                                                                                                                 tctcTATTCTGTCCGCCGTTATTTTCCTTTTCATTTTCAGACGGGTTGCTAAAAAGGCAACGCCGGGTGTACCGGGAAAATTACAATGTCTGGTGGAAATCCTGGTAGGTTGGGTTAACGGCATCGTTAAAGAAAATTTCCATGGTCCGCGCGAGGTGGTGGCACCGATTGCCCTGACCATTTTCTGTTGGGTATTCATTATGAATGCCATCGACTTAATCCCTGTTGATTTCCTTCCGCAACTTGCCGGa        >  1:227781/1‑251 (MQ=255)                                                                                                                                                                                                                 tctcTATTCTGTCCGCCGTTATTTTCCTTTTCATTTTCAGACGGGTTGCTAAAAAGGCAACGCCGGGTGTACCGGGAAAATTACAATGTCTGGTGGAAATCCTGGTAGGTTGGGTTAACGGCATCGTTAAAGAAAATTTCCATGGTCCGCGCGAGGTGGTGGCACCGATTGCCCTGACCATTTTCTGTTGGGTATTCATTATGAATGCCATCGACTTAATCCCTGTTGATTTCCTTCCGCAACTTGCCGGa        >  1:170949/1‑251 (MQ=255)                                                                                                                                                                                                                 tctcTATTCTGTCCGCCGTTATTTTCCTTTTCATTTTCAGACGGGTTGCTAAAAAGGCAACGCCGGGTGTACCGGGAAAATTACAATGTCTGGTGGAAATCCTGGTAGGTTGGGTTAACGGCATCGTTAAAGAAAATTTCCATGGTCCGCGCGAGGTGGTGGCACCGATTGCCCTGACCATTTTCTGTTGGGTATTCATTATGAATGCCATCGACTTAATCCCTGTTGATTTCCTTCCGCAACTTGCCGGa        >  1:216698/1‑251 (MQ=255)                                                                                                                                                                                                                 tctcTATTCTGTCCGCCGTTATTTTCCTTTTCATTTTCAGACGGGTTGCTAAAAAGGCAACGCCGGGTGTACCGGGAAAATTACAATGTCTGGTGGAAATCCTGGTAGGTTGGGTTAACGGCATCGTTAAAGAAAATTTCCATGGTCCGCGCGAGGTGGTGGCACCGATTGCCCTGACCATTTTCTGTTGGGTATTCATTATGAATGCCATCGACTTAATCCCTGTTGATTTCCTTCCGCAACTTGCCGGa        >  1:770536/1‑251 (MQ=255)                                                                                                                                                                                                                 tctcTATTCTGTCCGCCGTTATTTTCCTTTTCATTTTCAGACGGGTTGCTAAAAAGGCAACGCCGGGTGTACCGGGAAAATTACAATGTCTGGTGGAAATCCTGGTAGGTTGGGTTAACGGCATCGTTAAAGAAAATTTCCATGGTCCGCGCGAGGTGGTGGCACCGATTGCCCTGACCATTTTCTGTTGGGTATTCATTATGAATGCCATCGACTTAATCCCTGTTGATTTCCTTCCGCAACTTGCCGGa        >  1:95208/1‑251 (MQ=255)                                                                                                                                                                                                                 tctcTATTCTGTCCGCCGTTATTTTCCTTTTCATTTTCAGACGGGTTGCTAAAAAGGCAACGCCGGGTGTACCGGGAAAATTACAATGTCTGGCGGAAATCCTGGTAGGTTGGGTTAACGGCATCGTTAAAGAAAATTTCCATGGTCCGCGCGAGGCGGTGGCACCGATTGCCCTGACCATTTTCTGTTGGGTATTCATTATGAATGCCATCGACTTAATCCCTGTTGATTTCCTTCTGCAACTTGCCGGa        >  1:706461/1‑251 (MQ=255)                                                                                                                                                                                                                  ctctATTCTGTCCGCCGTTATTTTCCTTTTCATTTTCAGACGGGTTGCTAAAAAGGCAACGCCGGGTGTACCGGGAAAATTACAATGTCTGGTGGAAATCCTGGTAGGTTGGGTTAACGGCATCGTTAAAGAAAATTTCCATGGTCCGCGCGAGGTGGTGGCACCGATTGCCCTGACCATTTTCTGTTGGGTATTCATTATGAATGCCATCGACTTAATCCCTGTTGATTTCCTTCCGCAACTTGCCGGat       >  1:570032/1‑251 (MQ=255)                                                                                                                                                                                                                  ctctATTCTGTCCGCCGTTATTTTCCTTTTCATGTTCAGACGGGTTGCTAAAAAGGCAACGCCGGGTGTACCGGGAAAATTACAATGTCTGGTGGAAATCCTGGTAGGTTGGGTTAACGGCATCGTTAAAGAAAATTTCCATGGTCCGCGCGAGGTGGTGGCACCGATTGCCCTGACCATTTTCTGTTGGGTATTCATTATGAATGCCATCGACTTAATCCCTGTTGATTTCCTTCCGCAACTTGCCGGat       <  1:37773/251‑1 (MQ=255)                                                                                                                                                                                                                    ctATTCTGTCCGCCGTTATTTTCCTTTTCATTTTCAGACGGGTTGCTAAAAAGGCAACGCCGGGTGTACCGGGAAAATTACAATGTCTGGTGGAAATCCTGGTAGGTTGGGTTAACGGCATCGTTAAAGAAAATTTCCATGGTCCGCGCGAGGTGGTGGCACCGATTGCCCTGACCATTTTCTGTTGGGTATTCATTa                                                          >  1:566433/1‑198 (MQ=255)                                                                                                                                                                                                                       ttCTGTCCGCCGTTATTTTCCTTTTCATTTTCAGACGGGTTGCTAAAAAGGCAACGCCGGGTGTACCGGGAAAATTACAATGTCTGGTGGAAATCCTGGTAGGTTGGGTTAACGGCATCGTTAAAGAAAATTTCCATGGTCCGCGCGAGGTGGTGGCACCGATTGCCCTGACCATTTTCTGTTGGGTATTCATTATGAATGCCATCGACTTAATCCCTGTTGATTTCCTTCCGCAACTTGCCGGATTATTc  <  1:73077/251‑1 (MQ=255)                                                                                                                                                                                                                       ttCTGTCCGCCGTTATTTTCCTTTTCATTTTCAGACGGGTTGCTAAAAAGGCAACGCCGGGTGTACCGGGAAAATTACAATGTCTGGTGGAAATCCTGGTAGGTTGGGTTAACGGCATCGTTAAAGAAAATTTCCATGGTCCGCGCGAGGTGGTGGCACCGATTGCCCTGACCATTTTCTGTTGGGTATTCATTATGAATGCCATCGACTTAATCCCTGTTGATTTCCTTCCGCAACTTGCCGGATTATTc  <  1:111560/251‑1 (MQ=255)                                                                                                                                                                                                                       ttCTGTCCGCCGTTATTTTCCTTTTCATTTTCAGACGGGTTGCTAAAAAGGCAACGCCGGGTGTACCGGGAAAATTACAATGTCTGGTGGAAATCCTGGTAGGTTGGGTTAACGGCATCGTTAAAGAAAATTTCCATGGTCCGCGCGAGGTGGTGGCACCGATTGCCCTGACCATTTTCTGTTGGGTATTCATTATGAATGCCATCGACTTAATCCCTGTTGATTTCCTTCCGCAACTTGCCGGATTATTc  <  1:105921/251‑1 (MQ=255)                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                    GTAACATATTTTATTGTTTTCTTTACAGGTTTTTTTACAGCATTAATGCTAAATAATATTATCCCATTTCTGCTGAATAGGTCTTAAGTTTTATTTTACAAAGGGTTAACTATGTCTGAACAAACAACTTCAGAATATATCGGTCACCACTTATCTTTCCTTAAAACGGGGGACGGTTTCTGGAATATTCACGTTGATACCTTATTTTTCTCTATTCTGTCTGCCGTTATTTTCCTTTTCATTTTCAGACGGGTTGCTAAAAAGGCAACGCCGGGTGTACCGGGAAAATTACAATGTCTGGTGGAAATCCTGGTAGGTTGGGTTAACGGCATCGTTAAAGAAAATTTCCATGGTCCGCGCGAGGTGGTGGCACCGATTGCCCTGACCATTTTCTGTTGGGTATTCATTATGAATGCCATCGACTTAATCCCTGTTGATTTCCTTCCGCAACTTGCCGGATTATTC  >  1/482517‑482981

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑