Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	477,698	A→C	intergenic (+235/‑208)	unknown → / → unknown	Flavoprotein MioC/tRNA‑5‑carboxymethylaminomethyl‑2‑ thiouridine(34) synthesis protein MnmG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	477,698	0	A	C	100.0%	47.6 / NA	18	intergenic (+235/‑208)	unknown/unknown	Flavoprotein MioC/tRNA‑5‑carboxymethylaminomethyl‑2‑ thiouridine(34) synthesis protein MnmG
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (8/10);  total (8/10)

TAATCCTTTTGTTCAAAATAAAATCAGTCATTTTCTAAAACCTCTTGTGGGTAACTTCACATAAAATTGTGAAAAATCTGTTTTTATAACACGAATAAATTTTACAAAAGATCCAACTGTGGATAAATAACAGAGTTATACACAAGAAATTTGAGGAGGATCGGTAAGATCTTAACAACTTGTAGCATGATCCATCTCCTTCATTTACAACATAAAAAAGATCTTATCCAAGGTTTTAGTCGATCTTAATTATAGTAATAATAAACTTTATATATAGATAAAGATCTTATTACTTATTAACGTCGGATCCTTTCTTCAGATCCCGTCTTCTATTCCTCTCAAACTGACCTTTTATCCACACGGGATTTTCAGTAGAATAGCAGCCAATTTTTTTAAAGGATCAGATGATCCGTTACAGAATGCAAAAAAGAGATAAAAAA  >  1/477461‑477900                                                                                                                                                                                                                                              |                                                                                                                                                                                                           tAATCCTTTTGTTCAAAATAAAATCAGTCATTTTCTAAAACCTCTTGTGGGTAACTTCACATAAAATTGTGAAAAATCTGTTTTTATAACACGAATAAATTTTACAAAAGATCCAACTGTGGATAAATAACAGAGTTATACACAAGAAATTTGAGGAGGATCGGTAAGATCTTAACAACTTGTAGCATGATCCATCTCCTTCATTTACAACATAAAAAAGATCTTATCCAAGGTTTTCGTCGATCTTAAtt                                                                                                                                                                                               <  1:713715/251‑1 (MQ=255)   aTCCTTTTGTTCAAAATAAAATCAGTCATTTTCTAAAACCTCTTGTGGGTAACTTCACATAAAATTGTGAAAAATCTGTTTTTATAACACGAATAAATTTTACAAAAGATCCAACTGTGGATAAATAACAGAGTTATACACAAGAAATTTGAGGAGGATCGGTAAGATCTTAACAACTTGTAGCATGATCCATCTCCTTCATTTACAACATAAAAAAGATCTTATCCAAGGTTTTCGTCGATCTTAATtat                                                                                                                                                                                             <  1:848927/251‑1 (MQ=255)                aaataaaatCAGTCATTTTCTAAAACCTCTTGTGGGTAACTTCACATAAAATTGTGAAAAATCTGTTTTTATAACACGAATAAATTTTACAAAAGATCCAACTGTGGATAAATAACAGAGTTATACACAAGAAATTTGAGGAGGATCGGTAAGATCTTAACAACTTGTAGCATGATCCATCTCCTTCATTTACAACATAAAAAAGATCTTATCCAAGGTTTTCGTCGATCTTAATTATAGTAATAATAAAc                                                                                                                                                                                <  1:245006/251‑1 (MQ=255)                                                                        aaaaaTCTGTTTTTATAACACGAATAAATTTTACAAAAGATCCAACTGTGGATAAATAACAGAGTTATACACAAGAAATTTGAGGAGGATCGGTAAGATCTTAACAACTTGTAGCATGATCCATCTCCTTCATTTACAACATAAAAAAGATCTTATCCAAGGTTTTCGTCGATCTTAATTATAGTAATAATAAACTTTATATATAGATAAAGATCTTATTACTTATTAACGTCCGATCCTTTCTTCAGATc                                                                                                                        <  1:193102/251‑1 (MQ=255)                                                                                     tataACACGAATAAATTTTACAAAAGATCCAACTGTGGATAAATAACAGAGTTATACACAAGAAATTTGAGGAGGATCGGTAAGATCTTAACAACTTGTAGCATGATCCATCTCCTTCATTTACAACATAAAAAAGATCTTATCCAAGGTTTTCGTCGATCTTAATTATAGTAATAATAAACTTTATATATAGATAAAGATCTTATTACTTATTAACGTCCGATCCTTTCTTCAGATCCCGTCTTCTATTc                                                                                                           <  1:778382/251‑1 (MQ=255)                                                                                                             aGATCCAACTGTGGATAAATAACAGAGTTATACACAAGAAATTTGAGGAGGATCGGTAAGATCTTAACAACTTGTAGCATGATCCATCTCCTTCATTTACAACATAAAAAAGATCTTATCCAAGGTTTTCGTCGATCTTAAt                                                                                                                                                                                                >  1:888677/1‑142 (MQ=255)                                                                                                             aGATCCAACTGTGGATAAATAACAGAGTTATACACAAGAAATTTGAGGAGGATCGGTAAGATCTTAACAACTTGTAGCATGATCCATCTCCTTCATTTACAACATAAAAAAGATCTTATCCAAGGTTTTCGTCGATCTTAATTATAGTAATAATAAACTTTATATATAGATAAAGATCTTATTACTTATTAACGTCCGATCCTTTCTTCAGATc                                                                                                                        <  1:665302/214‑1 (MQ=255)                                                                                                                                  aCAGAGTTATACACAAGAAATTTGAGGAGGATCGGTAAGATCTTAACAACTTGTAGCATGATCCATCTCCTTCATTTACAACATAAAAAAGATCTTATCCAAGGTTTTCGTCGATCTTAATtat                                                                                                                                                                                             >  1:189332/1‑124 (MQ=255)                                                                                                                                     gagTTATACACAAGAAATTTGAGGAGGATCGGTAAGATCTTAACAACTTGTAGCATGATCCATCTCCTTCATTTACAACATAAAAAAGATCTTATCCAAGGTTTTCGTCGATCTTAATTATAg                                                                                                                                                                                           >  1:882061/1‑123 (MQ=255)                                                                                                                                       gTTATACACAAGAAATTTGAGGAGGATCGGTAAGATCTTAACAACTTGTAGCATGATCCATCTCCTTCATTTACAACATAAAAAAGATCTTATCCAAGGTTTTCGTCGATCTTAAtt                                                                                                                                                                                               <  1:621764/117‑1 (MQ=255)                                                                                                                                       gTTATACACAAGAAATTTGAGGAGGATCGGTAAGATCTTAACAACTTGTAGCATGATCCATCTCCTTCATTTACAACATAAAAAAGATCTTATCCAAGGTTTTCGTCGATCTTAATtat                                                                                                                                                                                             >  1:436152/1‑119 (MQ=255)                                                                                                                                                                          tCTTAACAACTTGTAGCATGATCCATCTCCTTCATTTACAACATAAAAAAGATCTTATCCAAGGTTTTCGTCGATCTTAATtat                                                                                                                                                                                             >  1:804761/1‑84 (MQ=255)                                                                                                                                                                                   cTTGTAGCATGATCCATCTCCTTCATTTACAACATAAAAAAGATCTTATCCAAGGTTTTCGTCGATCTTAATTATAGTAATAATAAACTTtatat                                                                                                                                                                         <  1:791798/95‑1 (MQ=255)                                                                                                                                                                                    ttGTAGCATGATCCATCTCCTTCATTTACAACATAAAAAAGATCTTATCCAAGGTTTTCGTCGATCTTAATTATAGTAATAATAAACTTTATATATAGATAAAGATcttattac                                                                                                                                                     >  1:646138/1‑114 (MQ=255)                                                                                                                                                                                        aGCATGATCCATCTCCTTCATTTACAACATAAAAAAGATCTTATCCAAGGTTTTCGTCGATCTTAAtt                                                                                                                                                                                               >  1:408503/1‑68 (MQ=255)                                                                                                                                                                                              aTCCATCTCCTTCATTTACAACATAAAAAAGATCTTATCCAAGGTTTTCGTCGATCTTAATTATAGTAATAATAAACTTTATATATAGATAAAGATCTTATTACTTATTAACGTCCGATCCTTTCTTCAGATCCCGTCTTCTATTCCTCTCAAACTGACCTTTTATCCACACGGGATTTTCAGTAGAATAGCAGCCAATTTTTTTAAAGGATCAGATGATCCGTTACAGAATGCAAAAAAGAGATaaaaaa  >  1:324945/1‑251 (MQ=255)                                                                                                                                                                                                         tCATTTACAACATAAAAAAGATCTTATCCAAGGTTTTCGTCGATCTTAATTATAg                                                                                                                                                                                           <  1:862225/55‑1 (MQ=255)                                                                                                                                                                                                                            gATCTTATCCAAGGTTTTCGTCGATCTTAATTATAGTAATAATAAACTTtatatat                                                                                                                                                                       <  1:649892/56‑1 (MQ=255)                                                                                                                                                                                                                                              |                                                                                                                                                                                                           TAATCCTTTTGTTCAAAATAAAATCAGTCATTTTCTAAAACCTCTTGTGGGTAACTTCACATAAAATTGTGAAAAATCTGTTTTTATAACACGAATAAATTTTACAAAAGATCCAACTGTGGATAAATAACAGAGTTATACACAAGAAATTTGAGGAGGATCGGTAAGATCTTAACAACTTGTAGCATGATCCATCTCCTTCATTTACAACATAAAAAAGATCTTATCCAAGGTTTTAGTCGATCTTAATTATAGTAATAATAAACTTTATATATAGATAAAGATCTTATTACTTATTAACGTCGGATCCTTTCTTCAGATCCCGTCTTCTATTCCTCTCAAACTGACCTTTTATCCACACGGGATTTTCAGTAGAATAGCAGCCAATTTTTTTAAAGGATCAGATGATCCGTTACAGAATGCAAAAAAGAGATAAAAAA  >  1/477461‑477900

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑