Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	472,441	A→G	D132G (GAC→GGC)	unknown →	Rhodanese‑related sulfurtransferase YibN

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	472,441	0	A	G	100.0%	208.0 / NA	69	D132G (GAC→GGC)	unknown	Rhodanese‑related sulfurtransferase YibN
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (43/26);  total (43/26)

CTTTAATGAACAACCAAAATGCCGTGGTTATCGACTTACGCTCCATTGATGAGTTTAACAAAGGCCATATCATAAACAGTTTAAACATCCTGCCTTCCGAAATTAAAAACAACAATATCGGAAAAATTGAACAGCACAAAGACATTCCGGTAATTTTAGCCTGTGCCGATTTCGTTTCTTCCCGTTCTTCCGGTGAAATTCTGGCAAAACAAGGTTTCAATCATGTTTATGTGCTAAGAGAAGACATCGGCGGCTGGCGTGCAGCAAATTTACCTTTAGTTAAAAAATAAAATAAGGACTCGAAAATGACCGAAGAAAACAAAGAAGTGACAAACCAAGAACAGGAAGTTCAAGCGGTGTTGCAAATTCAACGCATTTATGTGAAAGACGTTTCTTTTGAAGCACCAAACCTACCGCATATTTTCAACCAAGAATGGAAACCGCGTTTAAGTTTTGATTTATCTACCGAAACCGCTAAATTA  >  1/472198‑472679                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                               cTTTAATGAACAACCAAAATGCCGTGGTTATCGACTTACGCTCCATTGATGAGTTTAACAAAGGCCATATCATAAACAGTTTAAACATCCTGCCTTCCGAAATTAAAAACAACAATATCGGAAAAATTGAACAGCACAAAGACATTCCGGTAATTTTAGCCTGTGCCGATTTCGTTTCTTCCCGTTCTTCCGGTGAAATTCTGGCAAAACAAGGTTTCAATCATGTTTATGTGCTAAGAGAAGGCATcgg                                                                                                                                                                                                                                          >  1:106360/1‑250 (MQ=255)                 aaaTGCCGTGGTTATCGACTTACGCTCCATTGATGAGTTTAACAAAGGCCATATCATAAACAGTTTAAACATCCTGCCTTCCGAAATTAAAAACAACAATATCGGAAAAATTGAACAGCACAAAGACATTCCGGTAATTTTAGCCTGTGCCGATTTCGTTTCTTCCCGTTCTTCCGGTGAAATTCTGGCAAAACAAGGTTTCAATCATGTTTATGTGCTAAGAGAAGGCATCGGCGGCTGGCGTGCAGCaa                                                                                                                                                                                                                         <  1:363303/251‑1 (MQ=255)                   aTGCCGTGGTTATCGACTTACGCTCCATTGATGAGTTTAACAAAGGCCATATCATAAACAGTTTAAACATCCTGCCTTCCGAAATTAAAAACAACAATATCGGAAAAATTGAACAGCACAAAGACATTCCGGTAATTTTAGCCTGTGCCGATTTCGTTTCTTCCCGTTCTTCCGGTGAAATTCTGGCAAAACAAGGTTTCAATCATGTTTATGTGCTAAGAGAAGGCATCGGCGGCTGGCGTGCAGCAAAt                                                                                                                                                                                                                       >  1:405855/1‑251 (MQ=255)                                           ccATTGATGAGTTTAACAAAGGCCATATCATAAACAGTTTAAACATCCTGCCTTCCGAAATTAAAAACAACAATATCGGAAAAATTGAACAGCACAAAGACATTCCGGTAATTTTAGCCTGTGCCGATTTCGTTTCTTCCCGTTCTTCCGGTGAAATTCTGGCAAAACAAGGTTTCAATCATGTTTATGTGCTAAGAGAAGGCATCGGCGGCTGGCGTGCAGCAAATTTACCTTTAGTTAAaaaataaaat                                                                                                                                                                                               >  1:482741/1‑251 (MQ=255)                                            cATTGATGAGTTTAACAAAGGCCATATCATAAACAGTTTAAACATCCTGCCTTCCGAAATTAAAAACAACAATATCGGAAAAATTGAACAGCACAAAGACATTCCGGTAATTTTAGCCTGTGCCGATTTCGTTTCTTCCCGTTCTTCCGGTGAAATTCTGGCAAAACAAGGTTTCAATCATGTTTATGTGCTAAGAGAAGGCATCGGCGGCTGGCGTGCAGCAAATTTACCTTTAGTTAAAaaataaaata                                                                                                                                                                                              <  1:253169/251‑1 (MQ=255)                                              ttGATGAGTTTAACAAAGGCCATATCATAAACAGTTTAAACATCCTGCCTTCCGAAATTAAAAACAACAATATCGGAAAAATTGAACAGCACAAAGACATTCCGGTAATTTTAGCCTGTGCCGATTTCGTTTCTTCCCGTTCTTCCGGTGAAATTCTGGCAAAACAAGGTTTCAATCATGTTTATGTGCTAAGAGAAGGCATCGGCGGCTGGCGTGCAGCAAATTTACCTTTAGTTAAAAAATAAAATAAg                                                                                                                                                                                            <  1:605545/251‑1 (MQ=255)                                                     gTTTAACAAAGGCCATATCATAAACAGTTTAAACATCCTGCCTTCCGAAATTAAAAACAACAATATCGGAAAAATTGAACAGCACAAAGACATTCCGGTAATTTTAGCCTGTGCCGATTTCGTTTCTTCCCGTTCTTCCGGTGAAATTCTGGCAAAACAAGGTTTCAATCATGTTTATGTGCTAAGAGAAGGCATCGGCGGCTGGCGTGCAGCAAATTTACCTTTAGTTAAAAaataaaataa                                                                                                                                                                                             >  1:89052/1‑243 (MQ=255)                                                     gTTTAACAAAGGCCATATCATAAACAGTTTAAACATCCTGCCTTCCGAAATTAAAAACAACAATATCGGAAAAATTGAACAGCACAAAGACATTCCGGTAATTTTAGCCTGTGCCGATTTCGTTTCTTCCCGTTCTTCCGGTGAAATTCTGGCAAAACAAGGTTTCAATCATGTTTATGTGCTAAGAGAAGGCATCGGCGGCTGGCGTGCAGCAAATTTACCTTTAGTTAAAAAATAAAATAAGGACTCGa                                                                                                                                                                                     >  1:422310/1‑251 (MQ=255)                                                               ggCCATATCATAAACAGTTTAAACATCCTGCCTTCCGAAATTAAAAACAACAATATCGGAAAAATTGAACAGCACAAAGACATTCCGGTAATTTTAGCCTGTGCCGATTTCGTTTCTTCCCGTTCTTCCGGTGAAATTCTGGCAAAACAAGGTTTCAATCATGTTTATGTGCTAAGAGAAGGCATCGGCGGCTGGCGTGCAGCAAATTTACCTTTAGTTAAAAAATAAAATAAGGACTCGAAAATGACCga                                                                                                                                                                           >  1:333656/1‑251 (MQ=255)                                                                     atCATAAACAGTTTAAACATCCTGCCTTCCGAAATTAAAAACAACAATATCGGAAAAATTGAACAGCACAAAGACATTCCGGTAATTTTAGCCTGTGCCGATTTCGTTTCTTCCCGTTCTTCCGGTGAAATTCTGGCAAAACAAGGTTTCAATCATGTTTATGTGCTAAGAGAAGGCATCGGCGGCTGGCGTGCAGCAAATTTACCTTTAGTTAAAAAATAAAATAAGGACTCGAAAATGAcc                                                                                                                                                                             <  1:897783/243‑1 (MQ=255)                                                                     atCATAAACAGTTTAAACATCCTGCCTTCCGAAATTAAAAACAACAATATCGGAAAAATTGAACAGCACAAAGACATTCCGGTAATTTTAGCCTGTGCCGATTTCGTTTCTTCCCGTTCTTCCGGTGAAATTCTGGCAAAACAAGGTTTCAATCATGTTTATGTGCTAAGAGAAGGCATCGGCGGCTGGCGTGCAGCAAATTTACCTTTAGTTAAAAAATAAAATAAGGACTCGAAAATGAc                                                                                                                                                                              <  1:481093/242‑1 (MQ=255)                                                                     atCATAAACAGTTTAAACATCCTGCCTTCCGAAATTAAAAACAACAATATCGGAAAAATTGAACAGCACAAAGACATTCCGGTAATTTTAGCCTGTGCCGATTTCGTTTCTTCCCGTTCTTCCGGTGAAATTCTGGCAAAACAAGGTTTCAATCATGTTTATGTGCTAAGAGAAGGCATCGGCGGCTGGCGTGCAGCAAATTTACCTTTAGTTAAAAAATAAAATAAGGACTCGAAAATGACCGAAGaaaa                                                                                                                                                                     >  1:262785/1‑251 (MQ=255)                                                                     atCATAAACAGTTTAAACATCCTGCCTTCCGAAATTAAAAACAACAATATCGGAAAAATTGAACAGCACAAAGACATTCCGGTAATTTTAGCCTGTGCCGATTTCGTTTCTTCCCGTTCTTCCGGTGAAATTCTGGCAAAACAAGGTTTCAATCATGTTTATGTGCTAAGAGAAGGCATCGGCGGCTGGCGTGCAGCAAATTTACCTTTAGTTAAAAAATAAAATAAGGACTCGAAAATGACCGAAGaaaa                                                                                                                                                                     >  1:369354/1‑251 (MQ=255)                                                                     atCATAAACAGTTTAAACATCCTGCCTTCCGAAATTAAAAACAACAATATCGGAAAAATTGAACAGCACAAAGACATTCCGGTAATTTTAGCCTGTGCCGATTTCGTTTCTTCCCGTTCTTCCGGTGAAATTCTGGCAAAACAAGGTTTCAATCATGTTTATGTGCTAAGAGAAGGCATCGGCGGCTGGCGTGCAGCAAATTTACCTTTAGTTAAAAAATAAAATAAGGACTCGAAAATGACCGAAGaaaa                                                                                                                                                                     >  1:508375/1‑251 (MQ=255)                                                                     atCATAAACAGTTTAAACATCCTGCCTTCCGAAATTAAAAACAACAATATCGGAAAAATTGAACAGCACAAAGACATTCCGGTAATTTTAGCCTGTGCCGATTTCGTTTCTTCCCGTTCTTCCGGTGAAATTCTGGCAAAACAAGGTTTCAATCATGTTTATGTGCTAAGAGAAGGCATCGGCGGCTGGCGTGCAGCAAATTTACCTTTAGTTAAAAAATAAAATAAGGACTCGAAAATGACCGAAGaaaa                                                                                                                                                                     >  1:69106/1‑251 (MQ=255)                                                                     atCATAAACAGTTTAAACATCCTGCCTTCCGAAATTAAAAACAACAATATCGGAAAAATTGAACAGCACAAAGACATTCCGGTAATTTTAGCCTGTGCCGATTTCGTTTCTTCCCGTTCTTCCGGTGAAATTCTGGCAAAACAAGGTTTCAATCATGTTTATGTGCTAAGAGAAGGCATCGGCGGCTGGCGTGCAGCAAATTTACCTTTAGTTAAAAAATAAAATAAGGACTCGAAAATGACCGAAGaaaa                                                                                                                                                                     >  1:182375/1‑251 (MQ=255)                                                                     atCATAAACAGTTTAAACATCCTGCCTTCCGAAATTAAAAACAACAATATCGGAAAAATTGAACAGCACAAAGACATTCCGGTAATTTTAGCCTGTGCCGATTTCGTTTCTTCCCGTTCTTCCGGTGAAATTCTGGCAAAACAAGGTTTCAATCATGTTTATGTGCTAAGAGAAGGCATCGGCGGCTGGCGTGCAGCAAATTTACCTTTAGTTAAAAAATAAAATAAGGACTCGAAAATGACCGAAGaaaa                                                                                                                                                                     >  1:331589/1‑251 (MQ=255)                                                                     atCATAAACAGTTTAAACATCCTGCCTTCCGAAATTAAAAACAACAATATCGGAAAAATTGAACAGCACAAAGACATTCCGGTAATTTTAGCCTGTGCCGATTTCGTTTCTTCCCGTTCTTCCGGTGAAATTCTGGCAAAACAAGGTTTCAATCATGTTTATGTGCTAAGAGAAGGCATCGGCGGCTGGCGTGCAGCAAATTTACCTTTAGTTAAAAAATAAAATAAGGACTCGAAAATGAACGAAGaaaa                                                                                                                                                                     >  1:580930/1‑251 (MQ=255)                                                                     atCATAAACAGTTTAAACATCCTGCCTTCCGAAATTAAAAACAACAATATCGGAAAAATTGAACAGCACAAAGACATTCCGGTAATTTTAGCCTGTGCCGATTTCGTTTCTTCCCGTTCTTCCGGTGAAATTCTGGCAAAACAAGGTTTCAATCATGTTTATGTGCTAAAAGAAGGCATCGGCGGCTGGCGTGCAGCAAATTTACCTTTAGTTAAAAAATAAAATAAGGACTCGAAAATGACCGAAGaaaa                                                                                                                                                                     >  1:438860/1‑251 (MQ=255)                                                                               gTTTAAACATCCTGCCTTCCGAAATTAAAAACAACAATATCGGAAAAATTGAACAGCACAAAGACATTCCGGTAATTTTAGCCTGTGCCGATTTCGTTTCTTCCCGTTCTTCCGGTGAAATTCTGGCAAAACAAGGTTTCAATCATGTTTATGTGCTAAGAGAAGGCATCGGCGGCTGGCGTGCAGCAAATTTACCTTTAGTTAAAAAATAAAATAAGGACTCGAAAATGACCGAAGAAAACAAAGAAGTg                                                                                                                                                           >  1:279014/1‑251 (MQ=255)                                                                                     aCATCCTGCCTTCCGAAATTAAAAACAACAATATCGGAAAAATTGAACAGCACAAAGACATTCCGGTAATTTTAGCCTGTGCCGATTTCGTTTCTTCCCGTTCTTCCGGTGAAATTCTGGCAAAACAAGGTTTCAATCATGTTTATGTGCTAAGAGAAGGCATCGGCGGCTGGCGTGCAGCAAATTTACCTTTAGTTAAAAAATAAAATAAGGACTCGAAAATGACCGAAGAAAACAAAGAAGTGACAAAc                                                                                                                                                     <  1:197923/251‑1 (MQ=255)                                                                                             ccTTCCGAAATTAAAAACAACAATATCGGAAAAATTGAACAGCACAAAGACATTCCGGTAATTTTAGCCTGTGCCGATTTCGTTTCTTCCCGTTCTTCCGGTGAAATTCTGGCAAAACAAGGTTTCAATCATGTTTATGTGCTAAGAGAAGGCATCGGCGGCTGGCGTGCAGCAAATTTACCTTTAGTTAAAAAATAAAATAAGGACTCGAAAATGACCGAAGAAAACAAAGAAGTGACAAACCAAGAAc                                                                                                                                              <  1:880446/250‑1 (MQ=255)                                                                                                  cGAAATTAAAAACAACAATATCGGAAAAATTGAACAGCACAAAGACATTCCGGTAATTTTAGCCTGTGCCGATTTCGTTTCTTCCCGTTCTTCCGGTGAAATTCTGGCAAAACAAGGTTTCAATCATGTTTATGTGCTAAGAGAAGGCATCGGCGGCTGGCGTGCAGCAAATTTACCTTTAGTTAAAAAATAAAATAAGGACTCGAAAATGACCGAAGAAAACAAAGAAGTGACAAACCAAGAACAGGaac                                                                                                                                        <  1:566628/251‑2 (MQ=255)                                                                                                  cGAAATTAAAAACAACAATATCGGAAAAATTGAACAGCACAAAGACATTCCGGTAATTTTAGCCTGTGCCGATTTCGTTTCTTCCCGTTCTTCCGGTGAAATTCTGGCAAAACAAGGTTTCAATCATGTTTATGTGCTAAGAGAAGGCATCGGCGGCTGGCGTGCAGCAAATTTACCTTTAGTTAAAAAATAAAATAAGGACTCGAAAATGACCGAAGAAAACAAAGAAGTGACAAACCAAGAACAGGAAg                                                                                                                                        <  1:193206/251‑1 (MQ=255)                                                                                                         aaaaaCAACAATATCGGAAAAATTGAACAGCACAAAGACATTCCGGTAATTTTAGCCTGTGCCGATTTCGTTTCTTCCCGTTCTTCCGGTGAAATTCTGGCAAAACAAGGTTTCAATCATGTTTATGTGCTAAGAGAAGGCATCGGCGGCTGGCGTGCAGCAAATTTACCTTTAGTTAAAAAATAAAATAAGGACTCGAAAATGAc                                                                                                                                                                              >  1:583168/1‑206 (MQ=255)                                                                                                           aaaCAACAATATCGGAAAAATTGAACAGCACAAAGACATTCCGGTAATTTTAGCCTGTGCCGATTTCGTTTCTTCCCGTTCTTCCGGTGAAATTCTGGCAAAACAAGGTTTCAATCATGTTTATGTGCTAAGAGAAGGCATCGGCGGCTGGCGTGCAGCAAATTTACCTTTAGTTAAAAAATAAAATAAGGACTCGAAAATGACCGAAGAAAACAAAGAAGTGACAAACCAAGAACAGGAAGTTCAAGCgg                                                                                                                               >  1:155187/1‑251 (MQ=255)                                                                                                            gtagccgtggttgcctaccttGAACCGCACAAAGACATTCCGGTAATTTTAGCCTGTGCCGATTTCGTTTCTTCCCGTTCTTCCGGTGAAATTCTGGCAAAACAAGGTTTCAATCATGTTTATGTGCTAAGAGAAGGCATCGGCGGCTGGCGTGCAGCAAATTTACCTTTAGTTAAAAAATAAAATAAGGACTCGAAAATGACCGAAGAAAACAAAGAAGTGACAAACCAAGAACAGGAAGTTCAAGCgg                                                                                                                               <  1:883107/231‑1 (MQ=255)                                                                                                              caacaaTATCGGAAAAATTGAACAGCACAAAGACATTCCGGTAATTTTAGCCTGTGCCGATTTCGTTTCTTCCCGTTCTTCCGGTGAAATTCTGGCAAAACAAGGTTTCAATCATGTTTATGTGCTAAGAGAAGGCATCGGCGGCTGGCGTGCAGCAAATTTACCTTTAGTTAAAAAATAAAATAAGGACTCGAAAATGACCGAAGAAAACAAAGAAGTGACAAACCa                                                                                                                                                   <  1:339669/228‑1 (MQ=255)                                                                                                                   atatCGGAAAAATTGAACAGCACAAAGACATTCCGGTAATTTTAGCCTGTGCCGATTTCGTTTCTTCCCGTTCTTCCGGTGAAATTCTGGCAAAACAAGGTTTCAATCATGTTTATGTGCTAAGAGAAGGCATCGGCGGCTGGCGTGCAGCAAATTTACCTTTAGTTAAAAAATAAAATAAGGACTCGAAAATGACCGAAGAAAACAAAGAAGTGACAAACCAAGAACAGGAAGTTCAAGCGGTGTTGCaa                                                                                                                       <  1:577258/251‑1 (MQ=255)                                                                                                                       cGGAAAAATTGAACAGCACAAAGACATTCCGGTAATTTTAGCCTGTGCCGATTTCGTTTCTTCCCGTTCTTCCGGTGAAATTCTGGCAAAACAAGGTTTCAATCATGTTTATGTGCTAAGAGAAGGCATCGGCGGCTGGCGTGCAGCAAATTTACCTTTAGTTAAAAAATAAAATAAGGACTCGAAAATGACCGAAGAAAACAAAGAAGTGACAAACCAAGAACAGGAAGTTCAAGCGGTGTTGCAAATTc                                                                                                                   <  1:652989/251‑1 (MQ=255)                                                                                                                       cGGAAAAATTGAACAGCACAAAGACATTCCGGTAATTTTAGCCTGTGCCGATTTCGTTTCTTCCCGTTCTTCCGGTGAAATTCTGGCAAAACAAGGTTTCAATCATGTTTATGTGCTAAGAGAAGGCATCGGCGGCTGGCGTGCAGCAAATTTACCTTTAGTTAAAAAATAAAATAAGGACTCGAAAATGACCGAAGAAAACAAAGAAGTGACAAACCAAGAACAGGAAGTTCAAGCGGTGTTGCAAATTc                                                                                                                   <  1:35835/251‑1 (MQ=255)                                                                                                                         gAAAAATTGAACAGCACAAAGACATTCCGGTAATTTTAGCCTGTGCCGATTTCGTTTCTTCCCGTTCTTCCGGTGAAATTCTGGCAAAACAAGGTTTCAATCATGTTTATGTGCTAAGAGAAGGCATCGGCGGCTGGCGTGCAGCAAATTTACCTTTAGTTAAAAAATAAAATAAGGACTCGAAAATGACCGAAGAAAACAAAGAAGTGACAAACCAAGAACAGGAAGTTCAAGCGGTGTTGCAAATTCaa                                                                                                                 >  1:823388/1‑251 (MQ=255)                                                                                                                            aaaTTGAACAGCACAAAGACATTCCGGTAATTTTAGCCTGTGCCGATTTCGTTTCTTCCCGTTCTTCCGGTGAAATTCTGGCAAAACAAGGTTTCAATCATGTTTATGTGCTAAGAGAAGGCATCGGCGGCTGGCGTGCAGCAAATTTACCTTTAGTTAAAAAATAAAATAAGGACTCGAAAATGACCGAAGAAAACAAAGAAGTGACAAACCAAGAACAGGAAGTTCAAGCGGTGTTGCAAATTc                                                                                                                   >  1:128990/1‑246 (MQ=255)                                                                                                                                      gCACAAAGACATTCCGGTAATTTTAGCCTGTGCCGATTTCGTTTCTTCCCGTTCTTCCGGTGAAATTCTGGCAAAACAAGGTTTCAATCATGTTTATGTGCTAAGAGAAGGCATcgg                                                                                                                                                                                                                                          >  1:180565/1‑117 (MQ=255)                                                                                                                                      gCACAAAGACATTCCGGTAATTTTAGCCTGTGCCGATTTCGTTTCTTCCCGTTCTTCCGGTGAAATTCTGGCAAAACAAGGTTTCAATCATGTTTATGTGCTAAGAGAAGGCATCGGCGGCTGGCGTGCAGCAAATTTACCTTTAGTTAAaaaataaaat                                                                                                                                                                                               >  1:177891/1‑160 (MQ=255)                                                                                                                                      gCACAAAGACATTCCGGTAATTTTAGCCTGTGCCGATTTCGTTTCTTCCCGTTCTTCCGGTGAAATTCTGGCAAAACAAGGTTTCAATCATGTTTATGTGCTAAGAGAAGGCATCGGCGGCTGGCGTGCAGCAAATTTACCTTTAGTTAAAAAATAAAATAAGGACTCGAAAATGACCGAAGAAAACAAAGAAGTGACAAACCAAGAACAGGaa                                                                                                                                         <  1:637460/214‑1 (MQ=255)                                                                                                                                      gCACAAAGACATTCCGGTAATTTTAGCCTGTGCCGATTTCGTTTCTTCCCGTTCTTCCGGTGAAATTCTGGCAAAACAAGGTTTCAATCATGTTTATGTGCTAAGAGAAGGCATCGGCGGCTGGCGTGCAGCAAATTTACCTTTAGTTAAAAAATAAAATAAGGACTCGAAAATGACCGAAGAAAACAAAGAAGTGACAAACCAAGAACAGGAAGtt                                                                                                                                      <  1:861057/217‑1 (MQ=255)                                                                                                                                      gCACAAAGACATTCCGGTAATTTTAGCCTGTGCCGATTTCGTTTCTTCCCGTTCTTCCGGTGAAATTCTGGCAAAACAAGGTTTCAATCATGTTTATGTGCTAAGAGAAGGCATCGGCGGCTGGCGTGCAGCAAATTTACCTTTAGTTAAAAAATAAAATAAGGACTCGAAAATGACCGAAGAAAACAAAGAAGTGACAAACCAAGAACAGGAAGTTCAAGCGGTGTTGCaa                                                                                                                       <  1:307995/232‑1 (MQ=255)                                                                                                                                                   ccGGTAATTTTAGCCTGTGCCGATTTCGTTTCTTCCCGTTCTTCCGGTGAAATTCTGGCAAAACAAGGTTTCAATCATGTTTATGTGCTAAGAGAAGGCATCGGCGGCTGGCGTGCAGCAAATTTACCTTTAGTTAAAAAATAAAATAAGGACTCGAAAATGACCGAAGAAAACAAAGAAGTGACAAACCAAGAAc                                                                                                                                              >  1:403281/1‑196 (MQ=255)                                                                                                                                                        aaTTTTAGCCTGTGCCGATTTCGTTTCTTCCCGTTCTTCCGGTGAAATTCTGGCAAAACAAGGTTTCAATCATGTTTATGTGCTAAGAGAAGGCATCGGCGGCTGGCGTGCAGCAAATTTACCTTTAGTTAAAAAATAAAATAAGGACTCGAAAATGACCGAAGAAAACAAAGAAGTGACAAACCAAGAACAGGAAGTTCAAGCGGTGTTGCAAATTCAACGCATTTATGTGAAAGACGTTTCTTTTGAAg                                                                                  >  1:299053/1‑251 (MQ=255)                                                                                                                                                         aTTTTAGCCTGTGCCGATTTCGTTTCTTCCCGTTCTTCCGGTGAAATTCTGGCAAAACAAGGTTTCAATCATGTTTATGTGCTAAGAGAAGGCATCGGCGGCTGGCGTGCAGCAAATTTAcc                                                                                                                                                                                                                  >  1:41646/1‑122 (MQ=255)                                                                                                                                                         aTTTTAGCCTGTGCCGATTTCGTTTCTTCCCGTTCTTCCGGTGAAATTCTGGCAAAACAAGGTTTCAATCATGTTTATGTGCTAAGAGAAGGCATCGGCGGCTGGCGTGCAGCAAATTTACCTTTAGTTAAAAAATAAAATAAGGACTCGAAAATGACCGAAGAAAACAAAGAAGTGACAAACCAAGAGCAGGAAGTTCAAGCGGTGTTGCAAATTCAACGCATTTATGTGAAAGACGTTTCTTTTGAAg                                                                                  >  1:809089/1‑250 (MQ=255)                                                                                                                                                         aTTTTAGCCTGTGCCGATTTCGTTTCTTCCCGTTCTTCCGGTGAAATTCTGGCAAAACAAGGTTTCAATCATGTTTATGTGCTAAGAGAAGGCATCGGCGGCTGGCGTGCAGCAAATTTACCTTTAGTTAAAAAATAAAATAAGGACTCGAAAATGACCGAAGAAAACAAAGAAGTGACAAACCAAGAACAGGAAGTTCAAGCgg                                                                                                                               >  1:828321/1‑205 (MQ=255)                                                                                                                                                         aTTTTAGCCTGTGCCGATTTCGTTTCTTCCCGTTCTTCCGGTGAAATTCTGGCAAAACAAGGTTTCAATCATGTTTATGTGCTAAGAGAAGGCATCGGCGGCTGGCGTGCAGCAAATTTACCTTTAGTTAAAAAATAAAATAAGGACTCGAAAATGACCGAAGAAAACAAAGAAGTGACAAACCAAGAACAGGAAGTTCAAGCGGTGTTGCAAATTCAACGCATTTAtgt                                                                                                      <  1:812067/230‑1 (MQ=255)                                                                                                                                                         aTTTTAGCCTGTGCCGATTTCGTTTCTTCCCGTTCTTCCGGTGAAATTCTGGCAAAACAAGGTTTCAATCATGTTTATGTGCTAAGAGAAGGCATCGGCGGCTGGCGTGCAGCAAATTTACCTTTAGTTAAAAAATAAAATAAGGACTCGAAAATGACCGAAGAAAACAAAGAAGTGACAAACCAAGAACAGGAAGTTCAAGCGGTGTTGCAAATTCAACGCATTTATGTGAAAGACGTTTCTTTTGAAg                                                                                  >  1:728957/1‑250 (MQ=255)                                                                                                                                                         aTTTTAGCCTGTGCCGATTTCGTTTCTTCCCGTTCTTCCGGTGAAATTCTGGCAAAACAAGGTTTCAATCATGTTTATGTGCTAAGAGAAGGCATCGGCGGCTGGCGTGCAGCAAATTTACCTTTAGTTAAAAAATAAAATAAGGACTCGAAAATGACCGAAGAAAACAAAGAAGCGACAAACCAAGAACAGGAAGTTCAAGCGGTGTTGCAAATTCAACGCATTTATGTGAAAGa                                                                                                >  1:293995/1‑236 (MQ=255)                                                                                                                                                              aGCCTGTGCCGATTTCGTTTCTTCCCGTTCTTCCGGTGAAATTCTGGCAAAACAAGGTTTCAATCATGTTTATGTGCTAAGAGAAGGCATCGGCGGCTGGCGTGCAGCAAATTTACCTTTAGTTAAAAAATAAAATAAGGACTCGAAAATGACCGAAGAAAACAAAGAAGTGACAAACCAAGAACAGGAAGTTCAAGCGGTGTTGCAAATTCAACGCATTTATGTGa                                                                                                    >  1:214312/1‑227 (MQ=255)                                                                                                                                                                 cTGTGCCGATTTCGTTTCTTCCCGTTCTTCCGGTGAAATTCTGGCAAAACAAGGTTTCAATCATGTTTATGTGCTAAGAGAAGGCATCGGCGGCTGGCGTGCAGCAAATTTACCTTTAGTTAAAAAATAAAATAAGGACTCGAAAATGACCGAAGAAAACAAAGAAGTGACAAACCAAGAACAGGAAGTTCAAGCGGTGTTGCAAATTCAACGCATTTATGTGAAAGACGTTTCTTTTGAAGCACCAAAcc                                                                         <  1:482337/251‑1 (MQ=255)                                                                                                                                                                   gtgCCGATTTCGTTTCTTCCCGTTCTTCCGGTGAAATTCTGGCAAAACAAGGTTTCAATCATGTTTATGTGCTAAGAGAAGGCATCGGCGGCTGGCGTGCAGCAAATTTACCTTTAGTTAAAAAATAAAATAAGGACTCGAAAATGACCGAAGAAAACAAAGAAGTGACGAACCAAGAACAGGAAGTTCAAGCGGTGTTGCaa                                                                                                                       >  1:452002/1‑203 (MQ=255)                                                                                                                                                                   gtgCCGATTTCGTTTCTTCCCGTTCTTCCGGTGAAATTCTGGCAAAACAAGGTTTCAATCATGTTTATGTGCTAAGAGAAGGCATCGGCGGCTGGCGTGCAGCAAATTTACCTTTAGTTAAAAAATAAAATAAGGACTCGAAAATGACCGAAGAAAACAAAGAAGTGACAAACCAAGAACAGGAAGTTCAAGCGGTGTTGCaa                                                                                                                       >  1:21301/1‑203 (MQ=255)                                                                                                                                                                      ccGATTTCGTTTCTTCCCGTTCTTCCGGTGAAATTCTGGCAAAACAAGGTTTCAATCATGTTTATGTGCTAAGAGAAGGCATCGGCGGCTGGCGTGCAGCAAATTTACCTTTAGTTAAAAAATAAAATAAGGACTCGAAAATGACCGAAGAAAACAAAGAAGTGACAAACCAAGAACAGGAAGTTCAAGCGGTGTTGCAAATTCAACGCATTTATGTGAAAGACGTTTCTTTTGAAGCACCAAACCTACCg                                                                    <  1:110151/251‑1 (MQ=255)                                                                                                                                                                                      ccGTTCTTCCGGTGAAATTCTGGCAAAACAAGGTTTCAATCATGTTTATGTGCTAAGAGAAGGCATCGGCGGCTGGCGTGCAGCAAATTTACCTTTAGTTAAAAAATAAAATAAGGACTCGAAAATGACCGAAGAAAACAAAGAAGTGACAAACCAAGAACAGGAAGTTCAAGCGGTGTTGCAAATTCAACGCATTTATGTGAAAGACGTTTCTTTTg                                                                                     <  1:822082/218‑1 (MQ=255)                                                                                                                                                                                       cGTTCTTCCGGTGAAATTCTGGCAAAACAAGGTTTCAATCATGTTTATGTGCTAAGAGAAGGCATCGGCGGCTGGCGTGCAGCAAATTTACCTTTAGTTAAAAAATAAAATAAGGACTCGAAAATGACCGAAGAAAACAAAGAAGTGACAAACCAAGAACAGGAAGTTCAAGCGGTGTTGCAAATTCAACGCATTTATGTGAAAGACGTTTCTTTTGAAGCACCAAACCTACCGCATATTTTCAACCAAGa                                                   <  1:268937/251‑1 (MQ=255)                                                                                                                                                                                               cGGTGAAATTCTGGCAAAACAAGGTTTCAATCATGTTTATGTGCTAAGAGAAGGCATCGGCGGCTGGCGTGCAGCAAATTTACCTTTAGTTAAAAAATAAAATAAGGACTCGAAAATGACCGAAGAAAACAAAGAAGTGACAAACCAAGAACAGGAAGTTCAAGCGGTGTTGCAAATTCAACGCATTTATGTGAAAGACGTTTCTTTTGAAGCACCAAACCTACCGCATATTTTCAACCAAGAATGGAAAc                                           <  1:3106/251‑1 (MQ=255)                                                                                                                                                                                                                 aCAAGGTTTCAATCATGTTTATGTGCTAAGAGAAGGCATCGGCGGCTGGCGTGCAGCAAATTTACCTTTAGTTAAAAAATAAAATAAGGACTCGAAAATGACCGAAGAAAACAAAGAAGTGACAAACCAAGAACAGGAAGTTCAAGCGGTGTTGCAAATTCAACGCATTTATGTGAAAGACGTTTCTTTTGAAGCACCAAACCTACCGCATATTTTCAACCAAGAATGGAAACCGCGTTTAAGTTTTGAtt                         <  1:443380/251‑1 (MQ=255)                                                                                                                                                                                                                      gTTTCAATCATGTTTATGTGCTAAGAGAAGGCATCGGCGGCTGGCGTGCAGCAAATTTACCTTTAGTTAAAAAATAAAATAAGGACTCGAAAATGACCGAAGAAAACAAAGAAGTGACAAACCAAGAACAGGAAGTTCAAGCGGTGTTGCAAATTCAACGCATTTATGTGAAAGACGTTTCTTTTGAAGCACCAAACCTACCGCATATTTTCAACCAAGAATGGAAACCGCGTTTAAGTTTTGATTTAt                      >  1:737763/1‑249 (MQ=255)                                                                                                                                                                                                                      gTTTCAATCATGTTTATGTGCTAAGAGAAGGCATCGGCGGCTGGCGTGCAGCAAATTTACCTTTAGTTAAAAAATAAAATAAGGACTCGAAAATGACCGAAGAAAACAAAGAAGTGACAAACCAAGAACAGGAAGTTCAAGCGGTGTTGCAAATTCAACGCATTTATGTGAAAGACGTTTCTTTTGAAGCACCAAACCTACCGCATATTTTCAACCAAGAATGGAAACCGCGTTTAAGTTTTGATTTAt                      >  1:785437/1‑249 (MQ=255)                                                                                                                                                                                                                       tttCAATCATGTTTATGTGCTAAGAGAAGGCATCGGCGGCTGGCGTGCAGCAAATTTACCTTTAGTTAAAAAATAAAATAAGGACTCGAAAATGATCGAAGAAAACAAAGAAGTGACAAACCAAGAACAGGAAGTTCAAGCGGTGTTGCAAATTCAACGCATTTATGTGAAAGACGTTTCTTTTGAAGCACCAAACCTACCGCATATTTTCAACCAAGAATGGAAACCGCGTTTAAGTTTTGATTTATCt                    <  1:419738/250‑1 (MQ=255)                                                                                                                                                                                                                          cAATCATGTTTATGTGCTAAGAGAAGGCATCGGCGGCTGGCGTGCAGCAAATTTACCTTTAGTTAAAAAATAAAATAAGGACTCGAAAATGACCGAAGAAAACAAAGAAGTGACAAACCAAGAACAGGAAGTTCAAGCGGTGTTGCAAATTCAACGCATTTATGTGAAAGACGTTTCTTTTGAAGCACCAAACCTACCGCATATTTTCAACCAAGAATGGAAACCGCGTTTAAGTTTTGATTTATCTACCg                >  1:83413/1‑251 (MQ=255)                                                                                                                                                                                                                            aTCATGTTTATGTGCTAAGAGAAGGCATCGGCGGCTGGCGTGCAGCAAATTTACCTTTAGTTAAAAAATAAAATAAGGACTCGAAAATGACCGAAGAAAACAAAGAAGTGACAAACCAAGAACAGGAAGTTCAAGCGGTGTTGCAAAt                                                                                                                     >  1:432576/1‑148 (MQ=255)                                                                                                                                                                                                                            aTCATGTTTATGTGCTAAGAGAAGGCATCGGCGGCTGGCGTGCAGCAAATTTACCTTTAGTTAAAAAATAAAATAAGGACTCGAAAATGACCGAAGAAAACAAAGAAGTGACAAACCAAGAACAGGAAGTTCAAGCGGTGTTGCAAATTCAACGCATTTATGTGAAAGACGTTTCTTTTGAAGCACCAAACCTACCGCATATTTTCAACCAAGAATGGAAACCGCGTTTAAGTTTTGATTTATCTACCGaa              >  1:866209/1‑251 (MQ=255)                                                                                                                                                                                                                              cATGTTTATGTGCTAAGAGAAGGCATCGGCGGCTGGCGTGCAGCAAATTTACCTTTAGTTAAAAAATAAAATAAGGACTCGAAAATGACCGAAGAAAACAAAGGAGTGACAAACCAAGAACAGGAAGTTCAAGCGGTGTTGCAAATTCAACGCATTTATGTGAAAGACGTTTCTTTTGAAGCACCAAACCTACCGCATATTTTCAACCAAGAATGGAAACCGCGTTTAAGTTTTGATTTATCTACCGaaa             >  1:878478/1‑250 (MQ=255)                                                                                                                                                                                                                               aTGTTTATGTGCTAAGAGAAGGCATCGGCGGCTGGCGTGCAGCAAATTTACCTTTAGTTAAAAAATAAAATAAGGACTCGAAAATGACCGAAGAAAACAAAGAAGTGACAAACCAAGAACAGGAAGTTCAAGCGGTGTTGCAAATTCAACGCATTTATGTGAAAGACGTTTCTTTTGAAGCACCAAACCTACCGCATATTTTCAACCAAGAATGGAAACCGCGTTTAAGTTTTGATTTATCTACCGAAAc            >  1:101572/1‑250 (MQ=255)                                                                                                                                                                                                                                tGTTTATGTGCTAAGAGAAGGCATCGGCGGCTGGCGTGCAGCAAATTTACCTTTAGTTAAAAAATAAAATAAGGACTCGAAAATGACCGAAGAAAACAAAGAAGTGACAAACCAAGAACAGGAAGTTCAAGCGGTGTTGCAAATTCAACGCATTTATGTGAAAGACGTTTCTTTTGAAGCACCAAACCTACCGCATATTTTCAACCAAGAATGGAAACCGCGTTTAAGTTTTGATTTATCTACCGa               >  1:705395/1‑246 (MQ=255)                                                                                                                                                                                                                                      tgtgCTAAGAGAAGGCATCGGCGGCTGGCGTGCAGCAAATTTACCTTTAGTTAAAAAATAAAATAAGGACTCGAAAATGACCGAAGAAAACAAAGAAGTGACAAACCAAGAACAGGAAGTTCAAGCGGTGTTGCAAATTCAACGCATTTATGTGAAAGACGTTTCTTTTGAAGCACCAAACCTACCGCATATTTTCAACCAAGAATGGAAACCGCGTTTAAGTTTTGATTTATCTACCGAAACCGCTAAAt    <  1:130783/251‑1 (MQ=255)                                                                                                                                                                                                                                       gtgCTAAGAGAAGGCATCGGCGGCTGGCGTGCAGCAAATTTACCTTTAGTTAAAAAATAAAATAAGGACTCGAAAATGACCGAAGAAAACAAAGAAGTGACAAACCAAGAACAGGAAGTTCAAGCGGTGTTGCAAATTCAACGCATTTATGTGAAAGACGTTTCTTTTGAAGCACCAAACCTACCGCATATTTTCAACCAAGAATGGAAACCGCGTTTAAGTTTTGATTTATCTACCGAAACCGCTAAAtt   >  1:557569/1‑251 (MQ=255)                                                                                                                                                                                                                                       gtgCTAAGAGAAGGCATCGGCGGCTGGCGTGCAGCAAATTTACCTTTAGTTAAAAAATAAAATAAGGACTCGAAAATGACCGAAGAAAACAAAGAAGTGACAAACCAAGAACAGGAAGTTCAAGCGGTGTTGCAAATTCAACGCATTTATGTGAAAGACGTTTCTTTTGAAGCACCAAACCTACCGCATATTTTCAACCAAGAATGGAAACCGCGTTTAAGTTTTGATTTATCTACCGAAACCGCTAAAtt   >  1:675061/1‑251 (MQ=255)                                                                                                                                                                                                                                        tgCTAAGAGAAGGCATCGGCGGCTGGCGTGCAGCAAATTTACCTTTAGTTAAAAAATAAAATAAGGACTCGAAAATGACCGAAGAAAACAAAGAAGTGACAAACCAAGAACAGGAAGTTCAAGCGGTGTTGCaa                                                                                                                       >  1:528584/1‑134 (MQ=255)                                                                                                                                                                                                                                        tgCTAAGAGAAGGCATCGGCGGCTGGCGTGCAGCAAATTTACCTTTAGTTAAAAAATAAAATAAGGACTCGAAAATGACCGAAGAAAACAAAGAAGTGACAAACCAAGAACAGGAAGTTCAAGCGGTGTTGCAAATTCAACGCATTTATGTGAAAGACGTTTCTTTTGAAGCACCAAACCTACCGCATATTTTCAACCAAGAATGGAAACCGCGTTTAAGTTTTGATTTATCTACCGAAACCGCTAAATTa  >  1:529069/1‑251 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                               CTTTAATGAACAACCAAAATGCCGTGGTTATCGACTTACGCTCCATTGATGAGTTTAACAAAGGCCATATCATAAACAGTTTAAACATCCTGCCTTCCGAAATTAAAAACAACAATATCGGAAAAATTGAACAGCACAAAGACATTCCGGTAATTTTAGCCTGTGCCGATTTCGTTTCTTCCCGTTCTTCCGGTGAAATTCTGGCAAAACAAGGTTTCAATCATGTTTATGTGCTAAGAGAAGACATCGGCGGCTGGCGTGCAGCAAATTTACCTTTAGTTAAAAAATAAAATAAGGACTCGAAAATGACCGAAGAAAACAAAGAAGTGACAAACCAAGAACAGGAAGTTCAAGCGGTGTTGCAAATTCAACGCATTTATGTGAAAGACGTTTCTTTTGAAGCACCAAACCTACCGCATATTTTCAACCAAGAATGGAAACCGCGTTTAAGTTTTGATTTATCTACCGAAACCGCTAAATTA  >  1/472198‑472679

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑