Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	471,507	A→G	G71G (GGT→GGC)	unknown ←	Anaerobic C4‑dicarboxylate transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	471,507	0	A	G	100.0%	106.0 / NA	43	G71G (GGT→GGC)	unknown	Anaerobic C4‑dicarboxylate transporter
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (32/11);  total (32/11)

CCGCCGCAATCGGGCTTGCAACAATAGCAAATTGTGATGCGATAACCGCCATGGATAACGGACGCTCCGGGCGAATACCATTTTGACGGCTCACTTCAGCGATAACCGGT‑AATACGGAGTAAGCCACGTGACCGG‑TTCCTGCTAACAGCGTAAATGTCCAAGTTACCGCAGGTGCCATAAAAGTAATGTATTTC‑GGA‑TTA‑CGACGTAAAATATTAGTGGCAA‑TTTT‑GATCATGTAATCTAACCCACCGGCAGCCTGCATTGCCGCTGCCGCTGCAACTACCGCCATAATCATAAACATCACATCAATTGGCAAGCCGGCAGGTTTTAAACCGAACCCAAAGGACAAAATTGTAAGCCCCAAGCCACCAAATACACCTAAACCGATTCCGCCAACACGCGCACCGATTAAAATGCAGAGCAACACAATGGCGAACTGAAATAAAAACATAGCAGACATACTGCC  >  1/471263‑471725                                                                                                                                                                                                                                                            |                                                                                                                                                                                                                           ccgccgcAATCGGGCTTGCAACAATAGCAAATTGTGATGCGATAACCGCCATGGATAACGGACGCTCCGGGCGAATACCATTTTGACGGCTCACTTCAGCGATAACCGGT‑AATACGGAGTAAGCCACGTGACCGG‑TTCCTGCTAACAGCGTAAATGTCCAAGTTACCGCAGGTGCCATAAAAGTAATGTATTTC‑GGA‑TTA‑CGACGTAAAATATTAGTGGCAA‑TTTT‑GATCATGTAATCTAACCCGCCGGCa                                                                                                                                                                                                                      >  1:73575/1‑251 (MQ=255)      gcAATCGGGCTTGCAACAATAGCAAATTGTGATGCGATAACCGCCATGGATAACGGACGCTCCGGGCGAATACCATTTTGACGGCTCACTTCAGCGATAACCGGT‑AATACGGAGTAAGCCACGTGACCGG‑TTCCTGCTAACAGCGTAAATGTCCAAGTTACCGCAGGTGCCATAAAAGTAATGTATTTC‑GGA‑TTA‑CGACGTAAAATATTAGTGGCAA‑TTTT‑GATCATGTAATCTAACCCGCCGGCAGc                                                                                                                                                                                                                    >  1:692469/1‑248 (MQ=255)             ggCTTGCAACAATAGCAAATTGTGATGCGATAACCGCCATGGATAACGGACGCTCCGGGCGAATACCATTTTGACGGCTCACTTCAGCGATAACCGGA‑AATACGGAGTAAGCCACGTGACCGG‑TTCCTGCTAACAGCGTAAATGTCCAAGTTACCGCAGGTGCCATAAAAGTAATGTATTTC‑GGA‑TTA‑CGACGTAAAATATTAGTGGCAA‑TTTT‑GATCATGTAATCTAACCCGCCGGCAGCCTGCATtgc                                                                                                                                                                                                           >  1:644153/1‑250 (MQ=255)                         tAGCAAATTGTGATGCGATAACCGCCATGGATAACGGACGCTCCGGGCGAATACCATTTTGACGGCTCACTTCAGCGATAACCGGT‑AATACGGAGTAAGCCACGTGACCGG‑TTCCTGCTAACAGCGTAAATGTCCAAGTTACCGCAGGTGCCATAAAAGTAATGTATTTC‑GGA‑TTA‑CGACGTAAAATATTAGTGGCAA‑TTTT‑GATCATGTAATCTAACCCGCCGGCAGCCTGCATTGCCGCTGCCGCTGCa                                                                                                                                                                                              >  1:191628/1‑251 (MQ=255)                          aGCAAATTGTGATGCGATAACCGCCATGGATAACGGACGCTCCGGGCGAATACCATTTTGACGGCTCACTTCAGCGATAACCGGT‑AATACGGAGTAAGCCACGTGACCGG‑TTCCTGCTAACAGCGTAAATGTCCAAGTTACCGCAGGTGCCATAAAAGTAATGTATTTC‑GGA‑TTA‑CGACGTAAAATATTAGTGGCAA‑TTTT‑GATCATGTAATCTAACCCGCCGGCAGCCTGCATTGCCGCTGCCGCTGCaa                                                                                                                                                                                             >  1:704529/1‑251 (MQ=255)                            aaatttGTGATGCAATAACCGCCATGGATAACGGACGCTCCGGGCGAATACCATTTTGACGGCTCACTTCAGCGATAACCGGT‑AATACGGAGTAAGCCACGTGACCGG‑TTCCTGCTAACAGCGTAAATGTCCAAGTTACCGCAGGTGCCATAAAAGTAATGTATTTC‑GGA‑TTA‑CGACGTAAAATATTAGTGGCAA‑TTTT‑GATCATGTAATCTAACCCGCCGGCAGCCTGCATTGCCGCTGCCGCTGCAACt                                                                                                                                                                                           <  1:582325/247‑1 (MQ=255)                            aaaaTTGTGATGCGATACCCGCCATGGATAACGGACGCTCCGGGCGAATACCATTTTGACGGCTCACTTCAGCGATAACCGGT‑AATACGGAGTAAGCCACGTGACCGG‑TTCCTGCTAACAGCGTAAATGTCCAAGTTACCGCAGGTGCCATAAAAGTAATGTATTTC‑GGA‑TTA‑CGACGTAAAATATTAGTGGCAA‑TTTT‑GATCATGTAATCTAACCCGCCGGCAGCCTGCATTGCCGCTGCCGCTGCAACt                                                                                                                                                                                           <  1:438019/250‑1 (MQ=255)                                           aacccgcaaggaaTAACGGCCGCCCGGGGCAAATCCCTTTTTGACGCCTCACTTCAGC‑AAAACCCGTAAAAACGGATAAAGCCACGTGACCGT‑TCCCTGCAAAAAGCGAAAATGTCAAATTTCCCGCGGGTGCAAAAAAAGTAATGTATTCC‑GGA‑TTA‑CGACGAAAAATATTAGT‑GCAATTTTT‑GATCATGTAATCAACCCCGCCGGCAGCCTGCATTGCCGCGGCCGCTGCAACTACCGCCATAATCATa                                                                                                                                                                            <  1:3732/239‑1 (MQ=255)                                                     ggATAACGGACGCTCCGGGCGAATACCATTTTGACGGCTCACTTCAGCGATAACCGGT‑AATACGGAGTAAGCCACGTGACCGG‑TTCCTGCTAACAGCGTAAATGTCCAAGTTACCGCAGGTGCCATAAAAGTAATGTATTTC‑GGA‑TTA‑CGACGTAAAATATTAGTGGCAA‑TTTT‑GATCATGTAATCTAACCCGCCGGCAGCCTGCAt                                                                                                                                                                                                              >  1:248892/1‑207 (MQ=255)                                                     ggATAACGGACGCTCCGGGCGAATACCATTTTGACGGCTCACTTCAGCGATAACCGGT‑AATACGGAGTAAGCCACGTGACCGG‑TTCCTGCTAACAGCGTAAATGTCCAAGTTACCGCAGGTGCCATAAAAGTAATGTATTTC‑GGA‑TTA‑CGACGTAAAATATTAGTGGCAA‑TTTT‑GATCATGTAATCTAACCCGCCGGCAGCCTGCATTGCCGCTGCCGCTGCAACTACCGCCATAATCATAAacatcacat                                                                                                                                                                  >  1:269593/1‑251 (MQ=255)                                                     ggATAACGGACGCTCCGGGCGAATACCATTTTGACGGCTCACTTCAGCGATAACCGGT‑AATACGGAGTAAGCCACGTGACCGG‑TTCCTGCTAACAGCGTAAATGTCCAAGTTACCGCAGGTGCCATAAAAGTAATGTATTTC‑GGA‑TTA‑CGACGTAAAATATTAGTGGCAA‑TTTT‑GATCATGTAATCTAACCCGCCGGCAGCCTGCATTGCCGCTGCCGCTGCAACTACCGCCATAATCATAAacatcacat                                                                                                                                                                  >  1:413116/1‑251 (MQ=255)                                                      gATAACGGACGCTCCGGGCGAATACCATTTTGACGGCTCACTTCAGCGATAACCGGT‑AATACGGAGTAAGCCACGTGACCGG‑TTCCTGCTAACAGCGTAAATGTCCAAGTTACCGCAGGTGCCATAAAAGTAATGTATTTC‑GGA‑TTA‑CGACGTAAAATATTAGTGGCAA‑TTTT‑GATCATGTAATCTAACCCGCCGGCAGCCTGCATTGCCGCTGCCGCTGCAACTACCGCCATAATCATAAacatcacat                                                                                                                                                                  >  1:81320/1‑250 (MQ=255)                                                           cGGACGCTCCGGGCGAATACCATTTTGACGGCTCACTTCAGCGATAACCGGT‑AATACGGAGTAAGCCACGTGACCGG‑TTCCTGCTAACAGCGTAAATGTCCAAGTTACCGCAGGTGCCATAAAAGTAATGTATTTC‑GGA‑TTA‑CGACGTAAAATATTAGTGGCAA‑TTTT‑GATCATGTAATCTAACCCGCCGGCAGCCTGCATTGCCGCTGCCGCTGCAACTACCGCCATAATCATAAACATCACATCAATTg                                                                                                                                                            >  1:637700/1‑251 (MQ=255)                                                                         gAATACCATTTTGACGGCTCACTTCAGCGATAACCGGT‑AATACGGAGTAAGCCACGTGACCGG‑TTCCTGCTAACAGCGTAAATGTCCAAGTTACCGCAGGTGCCATAAAAGTAATGTATTTC‑GGA‑TTA‑CGACGTAAAATATTAGTGGCAA‑TTTT‑GATCATGTAATCTAACCCGCCGGCAGCCTGCATTGCCGCTGCCGCTGCAACTACCGCCATaa                                                                                                                                                                                 >  1:93008/1‑216 (MQ=255)                                                                         gAATACCATTTTGACGGCTCACTTCAGCGATAACCGGT‑AATACGGAGTAAGCCACGTGACCGG‑TTCCTGCTAACAGCGTAAATGTCCAAGTTACCGCAGGTGCCATAAAAGTAATGTATTTC‑GGA‑TTA‑CGACGTAAAATATTAGTGGCAA‑TTTT‑GATCATGTAATCTAACCCGCCGGCAGCCTGCATTGCCGCTGCCGCTGCAACTACCGCCATAATCATAAACATCACATCAATTGGCAAGCCGGCAGGt                                                                                                                                              >  1:880987/1‑251 (MQ=255)                                                                              ccATTTTGACGGCTCACTTCAGCGATAACCGGT‑AATACGGAGTAAGCCACGTGACCGG‑TTCCTGCTAACAGCGTAAATGTCCAAGTTACCGCAGGTGCCATAAAAGTAATGTATTTC‑GGA‑TTA‑CGACGTAAAATATTAGTGGCAA‑TTTT‑GATCATGTAATCTAACCCGCCGGCAGCCTGCATTGCCGCTGCCGCTGCAACTACCGCCATAATCATAAACATCACATCaa                                                                                                                                                               >  1:265857/1‑229 (MQ=255)                                                                                                                     ggAGAAAGCCACG‑GGCCGGTTTCCTGCAAACAGCGAAAATTTCAAAGTTCCCGCGGGTGCAAAAAAAGTAAT‑TTTTTC‑CGA‑TTACCGACGAAAAATTTTGGTGCCAT‑TTTT‑GATAATTAAATCAACCCCGCCGGCAGCCTGCATGGCCGCTGCCGCTGCAACTACCGCCATAATCATAAACATCACATCAATTGGCAAGCCGGCAGGTTTTAAACCGAACCCAAAGGACAAAATTGTAAGCCCCAAGc                                                                                                      <  1:809641/248‑1 (MQ=255)                                                                                                                      gAGTAAGCCACGTGACCGG‑TTCCTGCTAACAGCGTAAATGTCCAAGTTACCGCAGGTGCCATAAAAGTAATGTATTTC‑GGA‑TTA‑CGACGTAAAATATTAGTGGCAA‑TTTT‑GATCATGTAATCTAACCCGCCGGCAGCCTGCATTGCCGCTGCCGCTGCAACTACCGCCATAATCATAAACATCACATCAATTGGCAAGCCGGCAGGTTTTAAAc                                                                                                                                       >  1:878435/1‑214 (MQ=255)                                                                                                                      gAGTAAGCCACGTGACCGG‑TTCCTGCTAACAGCGTAAATGTCCAAGTTACCGCAGGTGCCATAAAAGTAATGTATTTC‑GGA‑TTA‑CGACGTAAAATATTAGTGGCAA‑TTTT‑GATCATGTAATCTAACCCGCCGGCAGCCTGCATTGCCGCTGCCGCTGCAACTACCGCCATAATCATAAACATCACATCAATTGGCAAGCCGGCAGGTTTTAAACCGAACCCAAAGGACAAAATTGTAAGCCCCAAGccac                                                                                                   >  1:799607/1‑250 (MQ=255)                                                                                                                        gTAAGCCACGTGACCGG‑TTCCTGCTAACAGCGTAAATGTCCAAGTTACCGCAGGTGCCATAAAAGTAATGTATTTC‑GGA‑TTA‑CGACGTAAAATATTAGTGGCAA‑TTTT‑GATCATGTAATCTAACCCGCCGGCAGCCTGCATTGCCGCTGCCGCTGCAACTACCGCCATAATCATAAACATCACATCAATTGGCAAGCCGGCAGGTTTTAAACCGAACCCAAAgg                                                                                                                           >  1:633357/1‑224 (MQ=255)                                                                                                                                        g‑TTCCTGCTAACAGCGTAAATGTCCAAGTTACCGCAGGTGCCATAAAAGTAATGTATTTC‑GGA‑TTA‑CGACGTAAAATATTAGTGGCAA‑TTTT‑GATCATGTAATCTAACCCGCCGGCAGCCTGCATTGCCGCTGCCGCTGCAACTACCGCCATAATCATAAACATCACATCAATTGGCAAGCCGGCAGGTTTTAAACCGAACCCAAAGGACAAAATTGTAAGCCCCAAGCCACCAAATACACCTAAAc                                                                                    >  1:446020/1‑247 (MQ=255)                                                                                                                                        g‑TTCCTGCTAACAGCGTAAATGTCCAAGTTACCGCAGGTGCCATAAAAGTAATGTATTTC‑GGA‑TTA‑CGACGTAAAATATTAGTGGCAA‑TTTT‑GATCATGTAATCTAACCCGCCGGCAGCCTGCATTGCCGCTGCCGCTGCAACTACCGCCATAATCATAAACATCACATCAATTGGCAAGCCGGCAGGTTTTAAACCGAACCCAAAGGACAAAATTGTAAGCCCCAAGCCACCAAATACACCTAAACCGAt                                                                                >  1:696608/1‑251 (MQ=255)                                                                                                                                        g‑TTCCTGCTAACAGCGTAAATGTCCAAGTTACCGCAGGTGCCATAAAAGTAATGTATTTC‑GGA‑TTA‑CGACGTAAAATATTAGTGGCAA‑TTTT‑GATCATGTAATCTAACCCGCCGGCAGCCTGCATTGCCGCTGCCGCTGCAACTACCGCCATAATCATAAACATCACATCAATTGGCAAGCCGGCAGGTTTTAAACCGAACCCAAAGGACAAAATTGTAAGCCCCAAGCCACCAAATACACCTAAACCGAt                                                                                >  1:42644/1‑251 (MQ=255)                                                                                                                                             cTGCTAACAGCGTAAATGTCCAAGTTACCGCAGGTGCCATAAAAGTAATGTATTTC‑GGA‑TTA‑CGACGTAAAATATTAGTGGCAA‑TTTT‑GATCATGTAATCTAACCCGCCGGCAGCCTGCATTGCCGCTGCCGCTGCAACTACCGCCATAATCATAAACATCACATCAATTGGCAAGCCGGCAGGTTTTAAACCGAACCCAAAGGACAAAATTGTAAGCCCCAAGCCACCAAATACACCTAAACCGATTCCg                                                                            >  1:494170/1‑251 (MQ=255)                                                                                                                                               gCTAACAGCGTAAATGTCCAAGTTACCGCAGGTGCCATAAAAGTAATGTATTTC‑GGA‑TTA‑CGACGTAAAATATTAGTGGCAA‑TTTT‑GATCATGTAATCTAACCCGCCGGCAGCCTGCATTGCCGCTGCCGCTGCAACTACCGCCATAATCATAAACATCACATCAATTGGCAAGCCGGCAGGTTTTAAAcc                                                                                                                                      >  1:516307/1‑191 (MQ=255)                                                                                                                                                    cAGCGTAAATGTCCAAGTTACCGCAGGTGCCATAAAAGTAATGTATTTC‑GGA‑TTA‑CGACGTAAAATATTAGTGGCAA‑TTTT‑GATCATGTAATCTAACCCGCCGGCAGCCTGCATTGCCGCTGCCGCTGCAACTACCGCCATAATCATAAACATCACATCAATTGGCAAGCCGGCAGGTTTTAAACCGAACCCAAAGGACAAAATTGTAAGCCCCAAGCCACCAAAt                                                                                              >  1:104292/1‑226 (MQ=255)                                                                                                                                                    cAGCGTAAATGTCCAAGTTACCGCAGGTGCCATAAAAGTAATGTATTTC‑GGA‑TTA‑CGACGTAAAATATTAGTGGCAA‑TTTT‑GATCATGTAATCTAACCCGCCGGCAGCCTGCATTGCCGCTGCCGCTGCAACTACCGCCATAATCATAAACATCACATCAATTGGCAAGCCGGCAGGTTTTAAACCGAACCCAAAGGACAAAATTGTAAGCCCCAAGCCACCAAATac                                                                                            >  1:569241/1‑228 (MQ=255)                                                                                                                                                          aaaggcccaagtaccggcgggTGCCAAAAAAGTAAT‑TTTTTCGGGA‑TTA‑CGCGGAAAAATATTGGGGGCAT‑TTTT‑AACCATGTAACCAACCCCGCCGGCAGCCTGCATTGCCGCTGCCGCTGCAACTACCGCCATAATCATAAACATCACATCAATTGGCAAGCCGGCAGGTTTTAAACCGAACCCAAAGGACAAAATTGTAAGCCCCAAGCCACCAAATACACCTAAACCGATTCCGCCAACACGCGCAc                                                               <  1:439575/232‑1 (MQ=255)                                                                                                                                                              gTCCAAGTTACCGCAGGTGCCATAAAAGTAATGTATTTC‑GGA‑TTA‑CGACGTAAAATATTAGTGGCAA‑TTTT‑GATCATGTAATCTAACCCGCCGGCAGCCTGCATTGCCGCTGCCGCTGCAACTACCGCCATAATCATAAACATCACATCAATTGGCAAGCCGGCAGGTTTTAAACCGAACCCAAAGGACAAAATTGTAAGCCCCAAGCCACCAAATACACCTAAACCGAtt                                                                               >  1:498281/1‑231 (MQ=255)                                                                                                                                                                   atttcccgagggtgcaaaaaaaGTAATGTATTT‑‑GGATTTA‑CGACAAAAAATATTAGGGGCAT‑TTTT‑GATCATGTATTCTACCCCGCCGGCAGCCGGCATTGCCGCGGCCGCTGCAACTACCGCCATAATCATAAACATCACATCAATTGGCAAGCCGGCAGGTTTTAAACCGAACCCAAAGGACAAAATTGTAAGCCCCAAGCCACCAAATACACCTAAACCGATTCCGCCAACACGCGCACCGATTaaaa                                                      <  1:464212/233‑1 (MQ=255)                                                                                                                                                                    gTTACCGCAGGTGCCATAAAAGTAATGTATTTC‑GGA‑TTA‑CGACGTAAAATATTAGTGGCAA‑TTTT‑GATCATGTAATCTAACCCGCCGGCAGCCTGCATTGCCGCTGCCGCTGCAACTACCGCCATAATCATAAACATCACATCAATTGGCAAGCCGGCAGGTTTTAAACCGAACCCAAAGGACAAAATTGTAAGCCCCAAGCCACCAAATACACCTAAAc                                                                                    >  1:812442/1‑220 (MQ=255)                                                                                                                                                                                 ccATAAAAGTAATGTATTTC‑GGA‑TTA‑CGACGTAAAATATTAGTGGCAA‑TTTT‑GATCATGTAATCTAACCCGCCGGCAGCCTGCATTGCCGCTGCCGCTGCAACTACCGCCATAATCATAAACATCACATCAATTGGCAAGCCGGCAGGTTTTAAACCGAACCCAAAGGACAAAATTGTAAGCCCCAAGCCACCAAATACACCTAAACCGATTCCGCCAACACGCGCACCGATTaaaa                                                      >  1:18258/1‑237 (MQ=255)                                                                                                                                                                                 ccATAAAAGTAATGTATTTC‑GGA‑TTA‑CGACGTAAAATATTAGTGGCAA‑TTTT‑GATCATGTAATCTAACCCGCCGGCAGCCTGCATTGCCGCTGCCGCTGCAACTACCGCCATAATCATAAACATCACATCAATTGGCAAGCCGGCAGGTTTTAAACCGAACCCAAAGGACAAAATTGTAAGCCCCAAGCCACCAAATACACCTAAACCGATTCCGCCAACACGCGCACCGATTAAAATGCAGAGCAacac                                         >  1:431759/1‑250 (MQ=255)                                                                                                                                                                                       aaGTAATGTATTTC‑GGA‑TTA‑CGACGTAAAATATTAGTGGCAA‑TTTT‑GATCATGTAATCTAACCCGCCGGCAGCCTGCATTGCCGCTGCCGCTGCAACTACCGCCATAATCATAAACATCACATCAATTGGCAAGCCGGCAGGTTTTAAACCGAACCCAAAGGACAAAATTGTAAGCCCCAAGCCACCAAATACACCTAAACCGATTCCGCCAACACGCGCACCGATTAAAATGCAGAGCAACACAATGGCg                                  >  1:698622/1‑251 (MQ=255)                                                                                                                                                                                              gTATTTC‑GGA‑TTA‑CGACGTAAAATATTAGTGGCAA‑TTTT‑GATCATGTAATCTAACCCGCCGGCAGCCTGCATTGCCGCTGCCGCTGCAACTACCGCCATAATCATAAac                                                                                                                                                                         >  1:711930/1‑109 (MQ=255)                                                                                                                                                                                                aTTTC‑GGA‑TTA‑CGACGTAAAATATTAGTGGCAA‑TTTT‑GATCATGTAATCTAACCCGCCGGCAGCCTGCATTGCCGCTGCCGCTGCAACTACCGCCATAATCATAAACATCACATCAATTGGCAAGCCGGCAGGTTTTAAACCGAACCCAAAGGACAAAATTGTAAGCCCCAAGCCACCAAATACACCTAAACCGATTCCGCCAACACGCGCACCGATTAAAATGCAGAGCAACACAATGGCGAACTGAAAt                         >  1:627669/1‑251 (MQ=255)                                                                                                                                                                                                 ttttgggA‑TTA‑CGACAAAAAATATTAGTGGCAT‑TTTT‑GATCATGTAATCTACCCCGCCGGCAGCCTGCATTGCCGCTGCCGCTGCAACTACCGCCATAATCATAAacatcacat                                                                                                                                                                  <  1:87549/109‑1 (MQ=255)                                                                                                                                                                                                       gA‑TTA‑CGACGTAAAATATTAGTGGCAA‑TTTT‑GATCATGTAATCTAACCCGCCGGCAGCCTGCATTGCCGCTGCCGCTGCAACTACCGCCATAATCATAAACATCACATCAATTGGCAAGCCGGCAGGTTTTAAACCGAACCCAAAGGACAAAATTGTAAGCCCCAAGCCACCAAATACACCTAAACCGATTCCGCCAACACGCGCACCGATTAAAATGCAGAGCAACACAATGGCGAACTGAAATaaaaa                    >  1:648825/1‑250 (MQ=255)                                                                                                                                                                                                           taacgacaaaaaaTTTTAGGGGAAA‑TTTG‑GATAATGAAATCAACCCCGCCGGCAGCCTGCATTGCCGCTGCCGCTGCAACTACCGCCATAATCATAAACATCACATCAATTGGCAAGCCGGCAGGTTTTAAACCGAACCCAAAGGACAAAATTGTAAGCCCCAAGCCACCAAATACACCTAAACCGATTCCGCCAACACGCGCACCGATTAAAATGCAGAGCAACACAATGGCGAACTGAAATAAAAACAt                 <  1:156838/242‑1 (MQ=255)                                                                                                                                                                                                                  aaaaaaTATTAGTGACAT‑TTTT‑AATCATGAACTCAACCCCGCGGGCAGCCTGCATTGCCGCTGCCGCTGCAACTACCGCCATAATCATAAACATCACATCAATTGGCAAGCCGGCAGGTTTTAAACCGAACCCAAAGGACAAAATTGTAAGCCCCAAGCCACCAAATACACCTAAACCGATTCCGCCAACACGCGCACCGATTAAAATGCAGAGCAACACAATGGCGAACTGAAATAAAAACATAGCAGAc          <  1:44126/249‑1 (MQ=255)                                                                                                                                                                                                                         ttttggggaaa‑TTTTGGATCATGTAATCAACCCCGCCGGCAGCCTGCATTGCCGCTGCCGCTGCAACTACCGCCATAATCATAAACATCACATCAATTGGCAAGCCGGCAGGTTTTAAACCGAACCCAAAGGACAAAATTGTAAGCCCCAAGCCACCAAATACACCTAAACCGATTCCGCCAACACGCGCACCGATTAAAATGCAGAGCAACACAATGGCGAACTGAAATAAAAACATAGCAGACATACTg    <  1:374781/242‑1 (MQ=255)                                                                                                                                                                                                                           ttgggggaattttt‑GATCATTAAATCAACCCCGCCGGCAGCCTGCATTGCCGCTGCCGCTGCAACTACCGCCATAATCATAAACATCACATCAATTGGCAAGCCGGCAGGTTTTAAACCGAACCCAAAGGACAAAATTGTAAGCCCCAAGCCACCAAATACACCTAAACCGATTCCGCCAACACGCGCACCGATTAAAATGCAGAGCAACACAATGGCGAACTGAAATAAAAACATAGCAGACATACTGcc  <  1:711650/241‑1 (MQ=255)                                                                                                                                                                                                                                 cAA‑TTTT‑GATCATGTAACCTAACCCGCCGGCAGCCTGCATTGCCGCTGCCGCTGCAACTACCGCCATAATCATAAACATCACATCAATTGGCAAGCCGGCAGGTTTTAAACCGAACCCAAAGGACAAAATTGTAAGCCCCAAGCCACCAAATACACCTAAACCGATTCCGCCAACACGCGCACCGATTAAAATGCAGAGCAACACAAt                                      >  1:820753/1‑208 (MQ=255)                                                                                                                                                                                                                                                            |                                                                                                                                                                                                                           CCGCCGCAATCGGGCTTGCAACAATAGCAAATTGTGATGCGATAACCGCCATGGATAACGGACGCTCCGGGCGAATACCATTTTGACGGCTCACTTCAGCGATAACCGGT‑AATACGGAGTAAGCCACGTGACCGG‑TTCCTGCTAACAGCGTAAATGTCCAAGTTACCGCAGGTGCCATAAAAGTAATGTATTTC‑GGA‑TTA‑CGACGTAAAATATTAGTGGCAA‑TTTT‑GATCATGTAATCTAACCCACCGGCAGCCTGCATTGCCGCTGCCGCTGCAACTACCGCCATAATCATAAACATCACATCAATTGGCAAGCCGGCAGGTTTTAAACCGAACCCAAAGGACAAAATTGTAAGCCCCAAGCCACCAAATACACCTAAACCGATTCCGCCAACACGCGCACCGATTAAAATGCAGAGCAACACAATGGCGAACTGAAATAAAAACATAGCAGACATACTGCC  >  1/471263‑471725

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑