Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	468,999	G→T	H410Q (CAC→CAA)	unknown ←	Heterodimeric efflux ABC transporter, permease/ATP‑binding subunit 1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	468,999	0	G	T	100.0%	201.9 / NA	68	H410Q (CAC→CAA)	unknown	Heterodimeric efflux ABC transporter, permease/ATP‑binding subunit 1
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (29/39);  total (29/39)

ATAGACATCAGCGTTATGCAAAAGGGCGCGGGCAAGCGCTAAACGTTGAATTTGCCCGCCGGACAAATTGCTTCCGCGACTGAGCAACGGCATCTCCAAACCGCCGTTTTCCCGCACAAAATTCGCCAGATTCACTTGTTCCAAACATTGATAAATGACTTCGTCGGAGGCATTTGATAACATCATTAGCATATTTTCCCGCAAGCTGCCTTTGAAAATGTAACTGCTGTGACTTACCAGCGAAATGTGGCGATATAAGGAATGGCGGTTAATCTGTTTAATATCGATGCCGTTAAACAAAATTTCGCCTTGTTGCGCCTGATAGAACCCCATTAGTAAAGATACCAAGGTCGATTTGCCACAACCGCTTTTGCCCACGAAAACCGTTAACTGTTTCGGCTGGATGCTGAGGGTTAAGCCGTTAATGGC‑GGGTTTTTCCTCGGAATA‑GGCAAAATGTAAATCGTTAATTTGCACGGTGACTTCATTTT  >  1/468753‑469240                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                    ataGACATCAGCGTTATGCAAAAGGGCGCGGGCAAGCGCTAAACGTTGAATTTGCCCGCCGGACAAATTGCTTCCGCGACTGAGCAACGGCATCTCCAAACCGCCGTTTTCCCGCACAAAATTCGCCAGATTCACTTGTTCCAAACATTGATAAATGACTTCGTCGGAGACATTTGATAACACCATTAGCATATTTTCCCGCAAGCTGCCTTTGAAAATGTAACTGCTGTGACTTACCAGCGAAATTTgg                                                                                                                                                                                                                                                  >  1:438633/1‑250 (MQ=255)      cATCAGCGTTATGCAAAAGGGCGCGGGCAAGCGCTAAACGTTGAATTTGCCCGCCGGACAAATTGCTTCCGCGACTGAGCAACGGCATCTCCAAACCGCCGTTTTCCCGCACAAAATTCGCCAGATTCACTTGTTCCAAACATTGATAAATGACTTCGTCGGAGGCATTTGATAACACCATTAGCATATTTTCCCGCAAGCTGCCTTTGAAAATGTAACTGCTGTGACTTACCAGCGAAATTTGGCGatat                                                                                                                                                                                                                                            >  1:782960/1‑251 (MQ=255)           gCGTTATGCAAAAGGGCGCGGGCAAGCGCTAAACGTTGAATTTGCCCGCCGGACAAATTGCTTCCGCGACTGAGCAACGGCATCTCCAAACCGCCGTTTTCCCGCACAAAATTCGCCAGATTCACTTGTTCCAAACATTGATAAATGACTTCGTCGGAGGCATTTGATAACACCATTAGCATATTTTCCCGCAAGCTGCCTTTGAAAATGTAACTGCTGTGACTTACCAGCGAAATTTGGCGATATAAGGa                                                                                                                                                                                                                                       >  1:237166/1‑251 (MQ=255)            cGTTATGCAAAAGGGCGCGGGCAAGCGCTAAGCGTTGAATTTGCCCGCCGGACAAATTGCTTCCGCGACTGAGCAACGGCATCTCCAAACCGCCGTTTTCCCGCACAAAATTCGCCAGATTCACTTGTTCCAAACATTGATAAATGACTTCGTCGGAGGCATTTGATAACACCATTAGCATATTTTCCCGCAAGCTGCCTTTGAAAATGTAACTGCTGTGACTTACCAGCGAAATTTGGCGATATAAGGa                                                                                                                                                                                                                                       <  1:338501/250‑1 (MQ=255)            cGTTATGCAAAAGGGCGCGGGCAAGCGCTAAACGTTGAATTTGCCCGCCGGACAAATTGCTTCCGCGACTGAGCAACGGCATCTCCAAACCGCCGTTTTCCCGCACAAAATTCGCCAGATTCACTTGTTCCAAACATTGATAAATGACTTCGTCGGAGGCATTTGATAACACCATTAGCATATTTTCCCGCAAGCTGCCTTTGAAAATGTAACTGCTGTGACTTACCAGCGAAATTTGGCGATATAAgg                                                                                                                                                                                                                                        <  1:695526/249‑1 (MQ=255)            cGTTATGCAAAAGGGCGCGGGCAAGCGCTAAACGTTGAATTTGCCCGCCGGACAAATTGCTTCCGCGACTGAGCAACGGCATCTCCAAACCGCCGTTTTCCCGCACAAAATTCGCCAGATTCACTTGTTCCAAACATTGATAAATGACTTCGTCGGAGGCATTTGATAACACCATTAGCATATTTTCCCGCAAGCTGCCTTTGAAAATGTAACTGCTGTGACTTACCAGCGAAATTTGGCGATATAAGGa                                                                                                                                                                                                                                       <  1:449114/250‑1 (MQ=255)            cGTTATGCAAAAGGGCGCGGGCAAGCGCTAAACGTTGAATTTGCCCGCCGGACAAATTGCTTCCGCGACTGAGCAACGGCATCTCCAAACCGCCGTTTTCCCGCACAAAATTCGCCAGATTCACTTGTTCCAAACATTGATAAATGACTTCGTCGGAGGCATTTGATAACACCATTAGCATATTTTCCCGCAAGCTGCCTTTGAAAATGTAACTGCTGTGACTTACCAGCGAAATTTGGCGATATAAGGa                                                                                                                                                                                                                                       <  1:708949/250‑1 (MQ=255)               tATGCAAAAGGGCGCGGGCAAGCGCTAAACGTTGAATTTGCCCGCCGGACAAATTGCTTCCGCGACTGAGCAACGGCATCTCCAAACCGCCGTTTTCCCGCACAAAATTCGCCAGATTCACTTGTTCCAAACATTGATAAATGACTTCGTCGGAGGCATTTGATAACACCATTAGCATATTTTCCCGCAAGCTGCCTTTGAAAATGTAACTGCTGTGACTTACCAGCGAAATTTGGCGATATAAGGAATgg                                                                                                                                                                                                                                   <  1:893511/251‑1 (MQ=255)                aTGCAAAAGGGCGCGGGCAAGCGCTAAACGTTGAATTTGCCCGCCGGACAAATTGCTTCCGCGACTGAGCAACGGTATCTCCAAACCGCCGTTTTCCCGCACAAAATTCGCCAGATTCACTTGTTCCAAACATTGATAAATGACTTCGTCGGAGGCATTTGATAACACCATTAGCATATTTTCCCGCAAGCTGCCTTTGAAAATGTAACTGCTGTGACTTACCAGCGAAATTTGGCGATATAAgg                                                                                                                                                                                                                                        <  1:432241/245‑1 (MQ=255)                aTGCAAAAGGGCGCGGGCAAGCGCTAAACGTTGAATTTGCCCGCCGGACAAATTGCTTCCGCGACTGAGCAACGGCATCTCCAAACCGCCGTTTTCCCGCACAAAATTCGCCAGATTCACTTGTTCCAAACATTGATAAATGACTTCGTCGGAGGCATTTGATAACACCATTAGCATATTTTCCCGCAAGCTGCCTTTGAAAATGTAACTGCTGTGACTTACCAGCGAAATTTGGCGATATAAGGAATgg                                                                                                                                                                                                                                   >  1:111644/1‑250 (MQ=255)                aTGCAAAAGGGCGCGGGCAAGCGCTAAACGTTGAATTTGCCCGCCGGACAAATTGCTTCCGCGACTGAGCAACGGCATCTCCAAACCGCCGTTTTCCCGCACAAAATTCGCCAGATTCACTTGTTCCAAACATTGATAAATGACTTCGTCGGAGGCATTTGATAACACCATTAGCATATTTTCCCGCAAGCTGCCTTTGAAAATGTAACTGCTGTGACTTACCAGCGAAATTTGGCGATATAAGGAATgg                                                                                                                                                                                                                                   >  1:581391/1‑250 (MQ=255)                aTGCAAAAGGGCGCGGGCAAGCGCTAAACGTTGAATTTGCCCGCCGGACAAATTGCTTCCGCGACTGAGCAACGGCATCTCCAAACCGCCGTTTTCCCGCACAAAATTCGCCAGATTCACTTGTTCCAAACATTGATAAATGACTTCGTCGGAGGCATTTGATAACACCATTAGCATATTTTCCCGCAAGCTGCCTTTGAAAATGTAACTGCTGTGACTTACCAGCGAAATTTGGCGATATAAGGAATgg                                                                                                                                                                                                                                   >  1:661713/1‑250 (MQ=255)                  gCAAAAGGGCGCGGGCAAGCGCTAAACGTTGAATTTGCCCGCCGGACAAATTGCTTCCGCGACTGAGCAACGGCATCTCCAAACCGCCGTTTTCCCGCACAAAATTCGCCAGATTCACTTGTTCCAAACATTGATAAATGACTTCGTCGGAGGCATTTGATAACACCATTAGCATATTTTCCCGCAAGCTGCCTTTGAAAATGTAACTGCTGTGACTTACCAGCGAAATTTGGCGATATAAGGAATGGCgg                                                                                                                                                                                                                                >  1:535369/1‑251 (MQ=255)                   cAAAAGGGCGCGGGCAAGCGCTAAACGTTGAATTTGCCCGCCGGACAAATTGCTTCCGCGACTGAGCAACGGCATCTCCAAACCGCCGTTTTCCCGCACAAAATTCGCCAGATTCACTTGTTCCAAACATTGATAAATGACTTCGTCGGAGGCATTTGATAACACCATTAGCATATTTTCCCGCAAGCTGCCTTTGAAAATGTAACTGCTGTGACTTACCAGCGAAATTTGGCGATATAAGGAATGGCgg                                                                                                                                                                                                                                <  1:216007/250‑1 (MQ=255)                                  aaGCGTTAATCGTTGAATTTGCCCGCCGGCAAAATTGCTTCCGCGGATGAGCGTCGGCTTCTCCAAGCCGCCGTTTTCCCGCACAAAATTCGCCAGATTCACTTGTTCCAAACATTGATAAATGACTTCGTCGGAGGCATTTGATAACTCCATTAGCATAGTTTCCCGCAAGCTGGCTTTGAAAATGTAACTGCTGTGACTTACCAGCGAAATTTGGGGATATAAGGAATGGCGGTTAATCTGTTTAatat                                                                                                                                                                                                                <  1:263113/251‑1 (MQ=255)                                  aaGCGCTAACCGTTGAATTTGCCCGCCGGACAAATTGCTTCCGCGACTGAGCAACGGCATCTCCAAACCGCCGTTTTCCCGCACAAAATTCGCCAGATTCACTTGTTCCAAACATTGATAAATGACTTCGTCGGAGGCATTTGATAACACCATTAGCATATTTTCCCGCAAGCTGCCTTTGAAAATGTAACTGCTGTGACTTACCAGCGAAATTTGGCGATATAAGGAATGGCGGTTAATCTGTTTAatat                                                                                                                                                                                                                <  1:795849/251‑1 (MQ=255)                                  aaGCGCTAAACGTTGAATTTGCCCGCCGGACAAATTGCTTCCGCGACTGAGCAACGGCATCTCCAAACCGCCGTTTTCCCGCACAAAATTCGCCAGATTCACTTGTTCCAAACATTGATAAATGACTTCGTCGGAGGCATTTGATAACGCCATTAGCATATTTTCCCGCAAGCTGCCTTTGAAAATGTAACTGCTGTGACTTACCAGCGAAATTTGGCGATATAAGGAATGGCGGTTAATCTGTTTAatat                                                                                                                                                                                                                <  1:347158/251‑1 (MQ=255)                                  aaGCGCTAAACGTTGAATTTGCCCGCCGGACAAATTGCTTCCGCGACTGAGCAACGGCATCTCCAAACCGCCGTTTTCCCGCACAAAATTCGCCAGATTCACTTGTTCCAAACATTGATAAATGACTTCGTCGGAGGCATTTGATAACACCATTAGCATATTTTCCCGCAAGCTGCCTTTGAAAATGTAACTGCTGTGACTTACCAGCGAAATTTGGCGATATAAGGAATGGCGGTTAATCTGTTTAatat                                                                                                                                                                                                                <  1:696818/251‑1 (MQ=255)                                  aaGCGCTAAACGTTGAATTTGCCCGCCGGACAAATTGCTTCCGCGACTGAGCAACGGCATCTCCAAACCGCCGTTTTCCCGCACAAAATTCGCCAGATTCACTTGTTCCAAACATTGATAAATGACTTCGTCGGAGGCATTTGATAACACCATTAGCATATTTTCCCGCAAGCTGCCTTTGAAAATGTAACTGCTGTGACTTACCAGCGAAATTTGGCGATATAAGGAATGGCGGTTAATCTGTTTAatat                                                                                                                                                                                                                <  1:45191/251‑1 (MQ=255)                                  aaGCGCTAAACGTTGAATTTGCCCGCCGGACAAATTGCTTCCGCGACTGAGCAACGGCATCTCCAAACCGCCGTTTTCCCGCACAAAATTCGCCAGATTCACTTGTTCCAAACATTGATAAATGACTTCGTCGGAGGCATTTGATAACACCATTAGCATATTTTCCCGCAAGCTGCCTTTGAAAATGTAACTGCTGTGACTTACCAGCGAAATTTGGCGATATAAGGAATGGCGGTTAATCTGTTTAatat                                                                                                                                                                                                                <  1:211263/251‑1 (MQ=255)                                        tAAACGTTGAATTTGCCCGCCGGACAAATTGCTTCCGCGACTGAGCAACGGCATCTCCAAACCGCCGTTTTCCCGCACAAAATTCGCCAGATTCACTTGTTCCAAACATTGATAAATGACTTCGTCGGAGGCATTTGATAACACCATTAGCATATTTTCCCGCAAGCTGCCTTTGAAAATGTAACTGCTGTGACTTACCAGCGAAATTTGGCGATATAAGGAATGGCGGTTAATCTGTTTAATATCGATGc                                                                                                                                                                                                          <  1:333931/251‑1 (MQ=255)                                            cGTTGAATTTGCCCGCCGGACAAATTGCTTCCGCGACTGAGCAACGGCATCTCCAAACCGCCGTTTTCCCGCACAAAATTCGCCAGATTCACTTGTTCCAAACATTGATAAATGACTTCGTCGGAGGCATTTGATAACACCATTAGCATATTTTCCCGCAAGCTGCCTTTGAAAATGTAACTGCTGTGACTTACCAGCGAAATTTGGCGATATAAGGAATGGCGGTTAATc                                                                                                                                                                                                                          <  1:336250/231‑1 (MQ=255)                                            cGTTGAATTTGCCCGCCGGACAAATTGCTTCCGCGACTGAGCAACGGCATCTCCAAACCGCCGTTTTCCCGCACAAAATTCGCCAGATTCACTTGTTCCAAACATTGATAAATGACTTCGTCGGAGGCATTTGATAACACCATTAGCATATTTTCCCGCAAGCTGCCTTTGAAAATGTAACTGCTGTGACTTACCAGCGAAATTTGGCGATATAAGGAATGGCGGTTAATCTGTTTAatat                                                                                                                                                                                                                <  1:415274/241‑1 (MQ=255)                                                 aaTTTGCCCGCCGGACAAATTGCTTCCGCGACTGAGCAACGGCATCTCCAAACCGCCGTTTTCCCGCACAAAATTCGCCAGATTCACTTGTTCCAAACATTGATAAATGACTTCGTCGGAGGCATTTGATAACACCATTAGCATATTTTCCCGCAAGCTGCCTTTGAAAATGTAACTGCTGTGACTTACCAGCGAAATTTGGCGATATAAGGAATGGCGGTTAATCTGTTTAATATCGATGCCGTTAAAc                                                                                                                                                                                                  <  1:742587/250‑1 (MQ=255)                                                                               aCTGAGCAACGGCATCTCCAAACCGCCGTTTTCCCGCACAAAATTCGCCAGATTCACTTGTTCCAAACATTGATAAATGACTTCGTCGGAGGCATTTGATAACACCATTAGCATATTTTCCCGCAAGCTGCCTTTGAAAATGTAACTGCTGTGACTTACCAGCGAAATTTGGCGATATAAGGAATGGCGGTTAATCTGTTTAATATCGATGCCGTTAAACAAAATTTCGCCTTGTTGCGCCTGATAGAAc                                                                                                                                                                    <  1:392719/250‑1 (MQ=255)                                                                                cTGAGCAACGGCATCTCCAAACCGCCGTTTTCCCGCACAAAATTCGCCAGATTCACTTGTTCCAAACATTGATAAATGACTTCGTCGGAGGCATTTGATAACACCATTAGCATATTTTCCCGCAAGCTGCCTTTGAAAATGTAACTGCTGTGACTTACCAGCGAAATTTGGCGATATAAGGAATGGCGGTTAATCTGTTTAATATCGATGCCGTTAAACAAAATTTCGCCTTGTTGCGCCTGATAGAAccc                                                                                                                                                                  <  1:680915/251‑1 (MQ=255)                                                                                          gCATCTCCAAACCGCCGTTTTCCCGCACAAAATTCGCCAGATTCACTTGTTCCAAACATTGATAAATGACTTCGTCGGAGGCATTTGATAACACCATTAGCATATTTTCCCGCAAGCTGCCTTTGAAAATGTAACTGCTGTGACTTACCAGCGAAATTTGGCGATATAAGGAAt                                                                                                                                                                                                                                     >  1:516381/1‑174 (MQ=255)                                                                                           cATCTCCAAACCGCCGTTTTCCCGCACAAAATTCGCCAGATTCACTTGTTCCAAACATTGATAAATGACTTCGTCGGAGGCATTTGATAACACCATTAGCATATTTTCCCGCAAGCTGCCTTTGAAAATGTAACTGCTGTGACTTACCAGCGAAATTTGGCGATATAAGGAATGGCGGTTAATCTGTTTAATATCGATGCCGTTAAACAAAATTTCGCCTTGTTGCGCCTGATAGAACCCCATTAGTAAAg                                                                                                                                                       <  1:162948/251‑1 (MQ=255)                                                                                           cATCTCCAAACCGCCGTTTTCCCGCACAAAATTCGCCAGATTCACTTGTTCCAAACATTGATAAATGACTTCGTCGGAGGCATTTGATAACACCATTAGCATATTTTCCCGCAAGCTGCCTTTGAAAATGTAACTGCTGTGACTTACCAGCGAAATTTGGCGATATAAGGAATGGCGGTTAATCTGTTTAATATCGATGCCGTTAAACAAAATTTCGCCTTGTTGCGCCTGATAGAACCCCATTAGTAAAg                                                                                                                                                       <  1:549906/251‑1 (MQ=255)                                                                                             tctcCAAACCGCCGTTTTCCCGCACAAAATTCGCCAGATTCACTTGTTCCAAACATTGATAAATGACTTCGTCGGAGGCATTTGATAACACCATTAGCATATTTTCCCGCAAGCTGCCTTTGAAAATGTAACTGCTGTGACTTACCAGCGAAATTTGGCGATATAAGGAATGGCGGTTAATCTGTTTAATATCGATGCCGTTAAACAAAATTTCGCCTTGTTGCGCCTGATAGAACCCCATTAGTAAAGAt                                                                                                                                                     >  1:732553/1‑251 (MQ=255)                                                                                              ctcCAAACCGCCGTTTTCCCGCACAAAATTCGCCAGATTCACTTGTTCCAAACATTGATAAATGACTTCGTCGGAGGCATTTGATAACACCATTAGCATATTTTCCCGCAAGCTGCCTTTGAAAATGTAACTGCTGTGACTTACCAGCGAAATTTGGCGATATAAGGAATGGCGGTTAATCTGTTTAatat                                                                                                                                                                                                                <  1:724529/191‑1 (MQ=255)                                                                                              ctcCAAACCGCCGTTTTCCCGCACAAAATTCGCCAGATTCACTTGTTCCAAACATTGATAAATGACTTCGTCGGAGGCATTTGATAACACCATTAGCATATTTTCCCGCAAGCTGCCTTTGAAAATGTAACTGCTGTGACTTACCAGCGAAATTTGGCGATATAAGGAATGGCGGTTAATCTGTTTAATATCGATGCCGTTAAACAAAATTTCGCCTTGTTGCGCCTGATAGAACCCCATTAGTAAAGATa                                                                                                                                                    >  1:184770/1‑251 (MQ=255)                                                                                                       gccgTTTTCCCGCACAAAATTCGCCAGATTCACTTGTTCCAAACATTGATAAATGACTTCGTCGGAGGCATTTGATAACACCATTAGCATATTTTCCCGCAAGCTGCCTTTGAAAATGTAACTGCTGTGACTTACCAGCGAAATTTGGCGATATAAGGAATGGCGGTTAATCTGTTTAATATCGATGCCGTTAAACAAAATTTCGCCTTGTTGCGCCTGATAGAACCCCATTAGTaa                                                                                                                                                         <  1:96885/237‑1 (MQ=255)                                                                                                             ttctcGCACAAAATTCGCCAGATTCACTTGTTCCAAACATTGATAAATGACTTCGTCGGAGGCATTTGATAACACCATTAGCATATTTTCCCGCAAGCTGCCTTTGAAAATGTAACTGCTGTGACTTACCAGCGAAATTTGGCGATATAAGGAATGGCGGTTAATCTGTTTAatat                                                                                                                                                                                                                >  1:252301/5‑176 (MQ=255)                                                                                                             ttCCCGCACAAAATTCGCCAGATTCACTTGTTCCAAACATTGATAAATGACTTCGTCGGAGGCATTTGATAACACCATTAGCATATTTTCCCGCAAGCTGCCTTTGAAAATGTAACTGCTGTGACTTACCAGCGAAATTTGGCGATATAAGGAATGGCGGTTAATCTGTTTAATATCGATGCCg                                                                                                                                                                                                        <  1:472549/184‑1 (MQ=255)                                                                                                                       aaaTTCGCCAGATTCACTTGTTCCAAACATTGATAAATGACTTCGTCGGAGGCATTTGATAACACCATTAGCATATTTTCCCGCAAGCTGCCTTTGAAAATGTAACTGCTGTGACTTACCAGCGAAATTTGGCGATATAAGGAATGGCGGTTAATCTGTTTAATATCGATGCCGTTAAACAAAATTTCGCCTTGTTGCGCCTGATAGAACCCCATTAGTAAAGATAc                                                                                                                                                   >  1:572667/1‑227 (MQ=255)                                                                                                                        aaTTCGCCAGATTCACTTGTTCCAAACATTGATAAATGACTTCGTCGGAGGCATTTGATAACACCATTAGCATATTTTCCCGCAAGCTGCCTTTGAAAATGTAACTGCTGTGACTTACCAGCGAAATTTGGCGATATAAGGAATGGCGGTTAATCTGTTTAATATCGATGCCGTTAAACAAAATTTCGCCTTGTTGCGCCTGATAGAACCCCATTAGTAAAGATACCAAGGTCGATTTGCCACAACCGCtt                                                                                                                          <  1:81513/251‑1 (MQ=255)                                                                                                                              ccAGATTCACTTGTTCCAAACATTGATAAATGACTTCGTCGGAGGCATTTGATAACACCATTAGCATATTTTCCCGCAAGCTGCCTTTGAAAATGTAACTGCTGTGACTTACCAGCGAAATTTGGCGATATAAGGAATGGCGGTTAATCTGTTTAATATCGATGCCGTTAAACAAAATTTCGCCTTGTTGCGCCTGATAGAACCCCATTAGTAAAGATACCAAGGTCGATTTGCCACAACCGCTTTTGccc                                                                                                                    <  1:551840/251‑1 (MQ=255)                                                                                                                                  aTTCACTTGTTCCAAACATTGATAAATGACTTCGTCGGAGGCATTTGATAACACCATTAGCATATTTTCCCGCAAGCTGCCTTTGAAAATGTAACTGCTGTGACTTACCAGCGAAATTTGGCGATATAAGGAATGGCGGTTAATCTGTTTAATATCGATGCCGTTAAACAAAATTTCGCCTTGTTGCGCCTGATAGAACCCCATTAGTAAAGATACCAAGGt                                                                                                                                             >  1:893997/1‑222 (MQ=255)                                                                                                                                          gTTCCAAACATTGATAAATGACTTCGTCGGAGGCATTTGATAACACCATTAGCATATTTTCCCGCAAGCTGCCTTTGAAAATGTAACTGCTGTGACTTACCAGCGAAATTTGGCGATATAAGGAATGGCGGTTAATc                                                                                                                                                                                                                          >  1:781729/1‑137 (MQ=255)                                                                                                                                          gTTCCAAACATTGATAAATGACTTCGTCGGAGGCATTTGATAACACCATTAGCATATTTTCCCGCAAGCTGCCTTTGAAAATGTAACTGCTGTGACTTACCAGCGAAATTTGGCGATATAAGGAATGGCGGTTAATCTGTTTAATATCGATGCCGTTAAACAAAATTTCGCCTTGTTGCGCCTGATAGAACCCCATTAGTaa                                                                                                                                                         <  1:731796/202‑1 (MQ=255)                                                                                                                                            tCCAAACATTGATAAATGACTTCGTCGGAGGCATTTGATAACACCATTAGCATATTTTCCCGCAAGCTGCCTTTGAAAATGTAACTGCTGTGACTTACCAGCGAAATTTGGCGATATAAGGAATGGCGGTTAATCTGTTTAATATCGATGCCGTTAAACAAAATTTCGCCTTGTTGCGCCTGATAGAACCCCATTAGTAAAg                                                                                                                                                       >  1:688804/1‑202 (MQ=255)                                                                                                                                                      gATAAATGACTTCGTCGGAGGCATTTGATAACACCATTAGCATATTTTCCCGCAAGCTGCCTTTGAAAATGTAACTGCTGTGACTTACCAGCGAAATTTGGCGATATAAGGAATGGCGGTTAATCTGTTTAATATCGATGCCGTTAAACAAAATTTTGCCTTGTTGCGCCTGATAGAACCCCATTAGTAAAGATACCAAGGTCGATTTGCCACAACCGCttt                                                                                                                         >  1:359428/1‑222 (MQ=255)                                                                                                                                                       aTAAATGACTTCGTCGGAGGCATTTGATAACACCATTAGCATATTTTCCCGCAAGCTGCCTTTGAAAATGTAACTGCTGTGACTTACCAGCGAAATTTGGCGATATAAGGAATGGCGGTTAATCTGTTTAATATCGATGCCGTTAAACAAAATTTCGCCTTGTTGCGCCTGATAGAACCCCATTAGTAAAGATACCAAGGTCGATTTTCCACAACCGCTTTTGCCCACGAAAACCGTTAACTGTTTctg                                                                                             >  1:387173/1‑247 (MQ=255)                                                                                                                                                               cTTCGTCGGAGGCATTTGATAACACCATTAGCATATTTTCCCGCAAGCTGCCTTTGAAAATGTAACTGCTGTGACTTACCAGCGAAATTTGGCGATATAAGGAATGGCGGTTAATCTGTTTAatat                                                                                                                                                                                                                <  1:782670/126‑1 (MQ=255)                                                                                                                                                                  cgtcgGAGGCATTTGATAACACCATTAGCATATTTTCCCGCAAGCTGCCTTTGAAAATGTAACTGCTGTGACTTACCAGCGAAATTTGGCGATATAAGGAATGGCGGTTAATCTGTTTAATATCGATGCCGTTAAACAAAATTTCGCCTTGTTGCGCCTGATAGAACCCCATTAGTAAAGATACCAAGGTCGATTTGCCACAACCGCTTTTGCCCACGAAAACCGTTAACTGTTTCGGCTGGATGCTGAgg                                                                                <  1:360123/251‑1 (MQ=255)                                                                                                                                                                  cgtcgGAGGCATTTGATAACACCATTAGCATATTTTCCCGCAAGCTGCCTTTGAAAATGTAACTGCTGTGACTTACCAGCGAAATTTGGCGATATAAGGAATGGCGGTTAATCTGGTTAATATCGATGCCGTTAAACAAAATTTCCCCTTGTTGCGCCTGATAGAACCCCATTAGTAAAGATACCAAg                                                                                                                                               >  1:228601/1‑188 (MQ=255)                                                                                                                                                                           cATTTGATAACACCATTAGCATATTTTCCCGCAAGCTGCCTTTGAAAATGTAACTGCTGTGACTTACCAGCGAAATTTGGCGATATAAGGAATGGCGGTTAATCTGTTTAATATCGATGCCGTTAAACAAAATTTCGCCTTGTTGCGCCTGATAGAACCCCATTAGTAAAGATACCAAGGTCGATTTGCCACAACCGCTTTTGCCCACGAAAACCGTTAACTGTTTCGGCTGGATGCTGAGGGTTAAGcc                                                                        <  1:616928/250‑1 (MQ=255)                                                                                                                                                                            aTTTGATAACACCATTAGCATATTTTCCCGCAAGCTGCCTTTGAAAATGTAACTGCTGTGACTTACCAGCGAAATTTGGCGATATAGGGAATGGCGGTTAATCTGTTTAATATCGATGCCGTTAAACAAAATTTCGCCTTGTTGCGCCTGATAGAACCCCATTAGTAAAGATACCAAGGTCGATTTGCCACAACCGCTTTTGCCCACGAAAACCGTTAACTGTTTCGGCTGGATGCTGAGGGTTAAGc                                                                         >  1:546808/1‑248 (MQ=255)                                                                                                                                                                             tttGATAACACCATTAGCATATTTTCCCGCAAGCTGCCTTTGAAAATGTAACTGCTGTGACTTACCAGCGAAATTTGGCGATATAAGGAATGGCGGTTAATCTGTTTAATATCGATGCCGTTAAACAAAATTTCGCCTTGTTGCGCCTGATAGAACCCCATTAGTAAAGATACCAAGGTCGATTTGCCACAACCGCTTTTGCCCACGAAAACCGTTAACTGTTTCGGCTGGATGCTGAGGGTTAAGCCGtt                                                                     >  1:220155/1‑251 (MQ=255)                                                                                                                                                                               tGATAACACCATTAGCATATTTTCCCGCAAGCTGCCTTTGAAAATGTAACTGCTGTGACTTACCAGCGAAATTTGGCGATATAAGGAATGGCGGTTAATCTGTTTAATATCGATGCCGTTAAACAAAATTTTGCCTTGTTGCGCCTGATAGAACCCCATTAGGAAAGATACCAAGGTCGATTTTCCACAACCGCTTTTGCCCACCAAAACCCTTAACTGTTTCGGCTGGATGCTGAGGGTTAAGCCGTTaa                                                                   >  1:503245/1‑251 (MQ=255)                                                                                                                                                                               tGATAACACCATTAGCATATTTTCCCGCAAGCTGCCTTTGAAAATGTAACTGCTGTGACTTACCAGCGAAATTTGGCGATATAAGGAATGGCGGTTAATCTGTTTAATATCGATGCCGTTAAACAAAATTTCGCCTTGTTGCGCCTGATAGAACCCCATTAGTAAAGATACCAAGGTCGATTTGCCACAACCGCTTTTGCCCACGAAAACCGTTAACt                                                                                                    <  1:355211/218‑1 (MQ=255)                                                                                                                                                                               tGATAACACCATTAGCATATTTTCCCGCAAGCTGCCTTTGAAAATGTAACTGCTGTGACTTACCAGCGAAATTTGGCGATATAAGGAATGGCGGTTAATCTGTTTAATATCGATGCCGTTAAACAAAATTTCGCCTTGTTGCGCCTGATAGAACCCCATTAGTAAAGATACCAAGGTCGATTTGCCACAACCGCTTTTGCCCACGAAAACCGTTAACTGTTTCGGCTGGATGCTGAGGGTTAAGCCGTTaa                                                                   >  1:343401/1‑251 (MQ=255)                                                                                                                                                                               tGATAACACCATTAGCATATTTTCCCGCAAGCTGCCTTTGAAAATGTAACTGCTGTGACTTACCAGCGAAATTTGGCGATATAAGGAATGGCGGTTAATCTGTTTAATATCGATGCCGTTAAACAAAATTTCGCCTTGTTGCGCCTGATAGAACCCCATTAGTAAAGATACCAAGGTCGATTTGCCACAACCGCTTTTGCCCACGAAAACCGTTAACTGTTTCGGCTGGATGCTGAGGGTTAAGCCGTTaa                                                                   >  1:290315/1‑251 (MQ=255)                                                                                                                                                                               tGATAACACCATTAGCATATTTTCCCGCAAGCTGCCTTTGAAAATGTAACTGCTGTGACTTACCAGCGAAATTTGGCGATATAAGGAATGGCGGTTAATCTGTTTAATATCGATGCCGTTAAACAAAATTTCGCCTTGTTGCGCCTGATAGAACCCCATTAGTAAAGATACCAAGGTCGATTTGCCACAACCGCTTTTGCCCACGAAAACCGTTAACTGTTTCGGCTGGATGCTGAGGGGTAAGCCGTTaa                                                                   >  1:60444/1‑251 (MQ=255)                                                                                                                                                                                     caccatTAGCATATTTTCCCGCAAGCTGCCTTTGAAAATGTAACTGCTGTGACTTACCAGCGAAATTTGGCGATATAAGGAATGGCGGTTAATCTGTTTAATATCGATGCCGTTAAACAAAATTTCGCCTTGTTGCGCCTGATAGAACCCCATTAGTAAAGATACCAAGGTCGATTTGCCACAAc                                                                                                                               >  1:764948/4‑185 (MQ=255)                                                                                                                                                                                       ccatTAGCATATTTTCCCGCAAGCTGCCTTTGAAAATGTAACTGCTGTGACTTACCAGCGAAATTTGGCGATATAAGGAATGGCGGTTAATCTGTTTAATATCGATGCCGTTAAACAAAATTTCGCCTTGTTGCGCCTGATAGAACCCCATTAGTAAAGATACCAAGGTCGATTTGCCACAACCGCTTTTGCCCACGAAAACCGTTAACTGTTTCGGCTGGATGCTGAGGGt                                                                              >  1:797892/2‑232 (MQ=255)                                                                                                                                                                                                ttatctCCCGCAAGCTGCCTTTGAAAATGTAACTGCTGTGACTTACCAGCGAAATTTGGCGATATAAGGAATGGCGGTTAATCTGTTTAATATCGATGCCGTTAAACAAAATTTCGCCTTGTTGCGCCTGATAGAACCCCATTAGTAAAGATACCAAGGTCGATTTGCCACAACCGCTTTTGCCCACGAAAACCGTTAACTGTTTCGGCTGGATGCTGAGGGTTAAGCCGTTAATGGC‑GGGTTTTTCCTc                                                  <  1:61177/245‑1 (MQ=255)                                                                                                                                                                                                      cccGCAAGCTGCCTTTGAAAATGTAACTGCTGTGACTTACCAGCGAAATTTGGCGATATAAGGAATGGCGGTTAATCTGTTTAATATCGATGCCGTTAAACAAAATTTCGCCTTGTTGCGCCTGATAGAACCCCATTAGTAAAGATACCAAGGTCGATTTGCCACAACCGCTTTTGCCCACGAAAACCGTTAACTGTTTCGGCTGGATGCTGAGGGTTAAGc                                                                         <  1:267297/222‑1 (MQ=255)                                                                                                                                                                                                                gCCTTTGAAAATGTAACTGCTGTGACTTACCAGCGAAATTTGGCGATATAAGGAATGGCGGTTAATCTGTTTAATATCGATGCCGTTAAACAAAATTTCGCCTTGTTGCGCCTGATAGAACCCCATTAGTAAAGATACCAAGGTCGATTTGCCACAACCGCTTTTGCCCACGAAAACCGTTAAc                                                                                                     <  1:387901/184‑1 (MQ=255)                                                                                                                                                                                                                      gAAAATGTAACTGCTGTGACTTACCAGCGAAATTTGGCGATATAAGGAATGGCGGTTAATCTGTTTAATATCGATGCCGTTAAACAAAATTTCGCCTTGTTGCGCCTGATAGAACCCCATTAGTAAAGATACCAAGGTCGATTTGCCACAACCGCTTTTGCCCACGAAAACCGTTAACTGTTTCGGCTGGATGCTGAGGGTTAAGCCGTTAATGGC‑GGGGTTTTtctccggataaggaaaaaagaaaaagg                           >  1:500266/1‑228 (MQ=255)                                                                                                                                                                                                                          aTGTAACTGCTGTGACTTACCAGCGAAATTTGGCGATATAAGGAATGGCGGTTAATCTGTTTAATATCGATGCCGTTAAACAAAATTTCGCCTTGTTGCGCCTGATAGAACCCCATTAGTAAAGATACCAAGGTCGATTTGCCACAACCGCTTTTGCCCACGAAAACCGTTAACTGTTTCGGCTGGATGCTGAGGGTTAAGCCGTTAATGGC‑GGGTTTTTCCTCGGAATA‑GGCAAAATGTAAATc                            <  1:9681/245‑1 (MQ=255)                                                                                                                                                                                                                           tGTAACTGCTGTGACTTACCAGCGAAATTTGGCGATATAAGGAATGGCGGTTAATCTGTTTAATATCGATGCCGTTAAACAAAATTTCGCCTTGTTGCGCCTGATAGAACCCCATTAGTAAAGATACCAAGGTCGATTTGCCACAACCGCTTTTGCCCACGAAAACCGTTAACTGTTTCGGCTGGATGCTGAGGGTTAAGCCGTTAATGGC‑GGGTTTTTCCTCGGAATA‑GGc                                        <  1:165738/232‑1 (MQ=255)                                                                                                                                                                                                                           tGTAACTGCTGTGACTTACCAGCGAAATTTGGCGATATAAGGAATGGCGGTTAATCTGTTTAATATCGATGCCGTTAAACAAAATTTCGCCTTGTTGCGCCTGATAGAACCCCATTAGTAAAGATACCAAGGTCGATTTGCCACAACCGCTTTTGCCCACGAAAACCGTTAACTGTTTCGGCTGGATCCTGAGGGTTAAGCCGTTAATGGC‑GGGTTTTTCCTCGGAATA‑GGCAAAATGTAAATCGTTAAtt                     <  1:580783/251‑1 (MQ=255)                                                                                                                                                                                                                             tAACTGCTGTGACTTACCAGCGAAATTTGGCGATATAAGGAATGGCGGTTAATCTGTTTAATATCGATGCCGTTAAACAAAATTTCGCCTTGTTGCGCCTGATAGAACCCCATTAGTAAAGATACCAAGGTCGATTTGCCACAACCGCTTTTGCCCACGAAAACCGTTAACTGTTTCGGCTGGATGCTGAGGGTTAAGCCGTTAATGGCGGGGTTTTTCTCCGGAAAA‑GGACAAATTAAAAACGTTAAttt                    >  1:405005/1‑251 (MQ=255)                                                                                                                                                                                                                                ctgctgTGACTTACCAGCGAAATTTGGCGATATAAGGAATGGCGGTTAATCTGTTTAATATCGATGCCGTTAAACAAAATTTCGCCTTGTTGCGCCTGATAGAACCCCATTAGTAAAGATACCAAGGTCGATTTGCCACAACCGCTTTTGCCCACGAAAACCGTTAACTGTTTCGGCTGGATGCTGAGGGTTAAGCCGTTAATGGC‑GGGTTTTTCCTCGGAATA‑GGCAAAATGTAAATCGTTAATTTGCAc                <  1:625662/251‑1 (MQ=255)                                                                                                                                                                                                                                     gtgACTTACCAGCGAAATTTGGCGATATAAGGAATGGCGGTTAATCTGTTTAATATCGATGCCGTTAAACAAAATTTCGCCTTGTTGCGCCTGATAGAACCCCATTAGTAAAGATACCAAGGTCGATTTGCCACAACCGCTTTTGCCCACGAAAACCGTTAACTGTTTCGGCTGGATGCTGAGGGGTAAGCCGTTAATGGC‑GGGGTTTTTCTCCGGATAGGGCaaaaagaaaaagtttatttttctggggg            >  1:587058/1‑227 (MQ=255)                                                                                                                                                                                                                                                 cGAAATTTGGCGATATAAGGAATGGCGGTTAATCTGTTTAATATCGATGCCGTTAAACAAAATTTCGCCTTGTTGCGCCTGATAGAACCCCATTAGTAAAGATACCAAGGTCGATTTGCCACAACCGCTTTTGCCCACGAAAACCGTTAACTGTTTCGGCTGGATGCTGAGGGTTAAGCCGTTAATGGC‑GGGTTTTTCCTCGGAATA‑GGCAAAATGTAAATCGTTAATTTGCACGGTGACTTCAtttt  <  1:685890/248‑1 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                    ATAGACATCAGCGTTATGCAAAAGGGCGCGGGCAAGCGCTAAACGTTGAATTTGCCCGCCGGACAAATTGCTTCCGCGACTGAGCAACGGCATCTCCAAACCGCCGTTTTCCCGCACAAAATTCGCCAGATTCACTTGTTCCAAACATTGATAAATGACTTCGTCGGAGGCATTTGATAACATCATTAGCATATTTTCCCGCAAGCTGCCTTTGAAAATGTAACTGCTGTGACTTACCAGCGAAATGTGGCGATATAAGGAATGGCGGTTAATCTGTTTAATATCGATGCCGTTAAACAAAATTTCGCCTTGTTGCGCCTGATAGAACCCCATTAGTAAAGATACCAAGGTCGATTTGCCACAACCGCTTTTGCCCACGAAAACCGTTAACTGTTTCGGCTGGATGCTGAGGGTTAAGCCGTTAATGGC‑GGGTTTTTCCTCGGAATA‑GGCAAAATGTAAATCGTTAATTTGCACGGTGACTTCATTTT  >  1/468753‑469240

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑