Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	468,935	T→C	M432V (ATG→GTG)	unknown ←	Heterodimeric efflux ABC transporter, permease/ATP‑binding subunit 1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	468,935	0	T	C	100.0%	229.2 / NA	75	M432V (ATG→GTG)	unknown	Heterodimeric efflux ABC transporter, permease/ATP‑binding subunit 1
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (34/41);  total (34/41)

AATTGCAGAATAATTTCTTCGCTTTCTACATCGATGTTACTGGTAGCTTCATCGAAAATATAGACATCAGCGTTATGCAAAAGGGCGCGGGCAAGCGCTAAACGTTGAATTTGCCCGCCGGACAAATTGCTTCCGCGACTGAGCAACGGCATCTCCAAACCGCCGTTTTCCCGCACAAAATTCGCCAGATTCACTTGTTCCAAACATTGATAAATGACTTCGTCGGAGGCATTTGATAACATCATTAGCATATTTTCCCGCAAGCTGCCTTTGAAAATGTAACTGCTGTGACTTACCAGCGAAATGTGGCGATATAAGGAATGGCGGTTAATCTGTTTAATATCGATGCCGTTAAACAAAATTTCGCCTTGTTGCGCCTGATAGAACCCCATTAGTAAAGATACCAAGGTCGATTTGCCACAACCGCTTTTGCCCACGAAAACCGTTAACTGTTTCGGCTGGATGCTGAGGGTTAAGCCGTTAA  >  1/468694‑469177                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                   aaTTGCAGAATAATTTCTTCGCTTTCTACATCGATGTTACTGGTAGCTTCATCGAAAATATAGACATCAGCGTTATGCAAAAGGGCGCGGGCAAGCGCTAAACGTTGAATTTGCCCGCCGGACAAATTGCTTCCGCGACTGAGCAACGGCATCTCCAAACCGCCGTTTTCCCGCACAAAATTCGCCAGATTCACTTGTTCCAAACATTGATAAATGACTTCGTCGGAGGCATTTGATAACACCATTAGCat                                                                                                                                                                                                                                           <  1:640953/251‑1 (MQ=255)         aataatTTCTTCGCTTTCTACATCGATGTTACTGGTAGCTTCATCGAAAATATAGACATCAGCGTTATGCAAAAGGGCGCGGGCAAGCGCTAAACGTTGAATTTGCCCGCCGGACAAATTGCTTCCGCGACTGAGCAACGGCATCTCCAAACCGCCGTTTTCCCGCACAAAATTCGCCAGATTCACTTGTTCCAAACATTGATAAATGACTTCGTCGGAGGCATTTGATAACACCATTAGCATAtttt                                                                                                                                                                                                                                      <  1:247428/248‑1 (MQ=255)                          cTACATCGATGTTACTGGTAGCTTCATCGAAAATATAGACATCAGCGTTATGCAAAAGGGCGCGGGCAAGCGCTAAACGTTGAATTTGCCCGCCGGACAAATTGCTTCCGCGACTGAGCAACGGCATCTCCAAACCGCCGTTTTCCCGCACAAAATTCGCCAGATTCACTTGTTCCAAACATTGATAAATGACTTCGTCGGAGGCATTTGATAACACCATTAGCATATTTTCCCGCAAGCTGCCTTTGaaa                                                                                                                                                                                                                  <  1:706233/251‑1 (MQ=255)                          cTACATCGATGTTACTGGTAGCTTCATCGAAAATATAGACATCAGCGTTATGCAAAAGGGCGCGGGCAAGCGCTAAACGTTGAATTTGCCCGCCGGACAAATTGCTTCCGCGACTGAGCAACGGCATCTCCAAACCGCCGTTTTCCCGCACAAAATTCGCCAGATTCACTTGTTCCAAACATTGATAAATGACTTCGTCGGAGGCATTTGATAACACCATTAGCATATTTTCCCGCAAGCTGCCTTTGaaa                                                                                                                                                                                                                  >  1:736822/1‑251 (MQ=255)                           tACATCGATGTTACTGGTAGCTTCATCGAAAATATAGACATCAGCGTTATGCAAAAGGGCGCGGGCAAGCGCTAAACGTTGAATTTGCCCGCCGGACAAATTGCTTCCGCGACTGAGCAACGGCATCTCCAAACCGCCGTTTTCCCGCACAAAATTCGCCAGATTCACTTGTTCCAAACATTGATAAATGACTTCGTCGGAGGCATTTGATAACACCATTAGCATATTTTCCCGCAAGCTGCCTTTGaaaa                                                                                                                                                                                                                 <  1:174542/251‑1 (MQ=255)                            aCATCGATGTTACTGGTAGCTTCATCGAAAATATAGACATCAGCGTTATGCAAAAGGGCGCGGGCAAGCGCTAAACGTTGAATTTGCCCGCCGGACAAATTGCTTCCGCGACTGAGCAACGGCATCTCCAAACCGCCGTTTTCCCGCACAAAATTCGCCAGATTCACTTGTTCCAAACATTGATAAATGACTTCGTCGGAGGCATTTGATAACACCATTAGCATATTTTCCCGCAAGCTGCCTTTGAAAAt                                                                                                                                                                                                                >  1:504704/1‑251 (MQ=255)                                 gATGTTACTGGTAGCTTCATCGAAAATATAGACATCAGCGTTATGCAAAAGGGCGCGGGCAAGCGCTAAACGTTGAATTTGCCCGCCGGACAAATTGCTTCCGCGACTGAGCAACGGCATCTCCAAACCGCCGTTTTCCCGCACAAAATTCGCCAGATTCACTTGTTCCAAACATTGATAAATGACTTCGTCGGAGGCATTTGATAACACCATTAGCATATTTTCCCGCAATCTGCCTTTGAAAATGTaa                                                                                                                                                                                                            >  1:521712/1‑250 (MQ=255)                                  atgtagtgggTAGCATCATCAAAAAGATAGACATCAGCGTAATGCAAAAGGGAGCGGGCAAGCTCTAAACTTTTAATTGGCCCGCCGGAAAAATTGCTTCCGCGACTGAGCATCGGCATCTCCAAACCGCCGTTTTCCCGCTCAAAATTCGCCAGATTCACTTGTTCCAAACATTGAAAAATGACTTCGTCGGAGGCATTTGATAACACCATTAGCATATTTTCCCGCAAGCTGCCTTTGAAAATGTAAc                                                                                                                                                                                                           <  1:771085/242‑1 (MQ=255)                                  aTGTTACTGGTAGCTTCATCGAAAATATAGACATCAGCGTTATGCAAAAGGGCGCGGGCAAGCGCTAAACGTTGAATTTGCCCGCCGGACAAATTGCTTCCGCGACTGAGCAACGGCATCTCCAAACCGCCGTTTTCCCGCACAAAATTCGCCAGATTCACTTGTTCCAAACATTGATAAATGACTTCGTCGGAGGCATTTGATAACACCATTAGCATATTTTCCCGCAAGCTGCCTTTGAAAATGTaa                                                                                                                                                                                                            >  1:820185/1‑249 (MQ=255)                                  aTGTTACTGGTAGCTTCATCGAAAATATAGACATCAGCGTTATGCAAAAGGGCGCGGGCAAGCGCTAAACGTTGAATTTGCCCGCCGGACAAATTGCTTCCGCGACTGAGCAACGGCATCTCCAAACCGCCGTTTTCCCGCACAAAATTCGCCAGATTCACTTGTTCCAAACATTGATAAATGACTTCGTCGGAGGCATTTGATAACACCATTAGCATATTTTCCCGCAAGCTGCCTTTGAAAATGTaa                                                                                                                                                                                                            >  1:523214/1‑249 (MQ=255)                                          ggTAGCTTCATCGAAAATATAGACATCAGCGTTATGCAAAAGGGCGCGGGCAAGCGCTAAACGTTGAATTTGCCCGCCGGACAAATTGCTTCCGCGACTGAGCAACGGCATCTCCAAACCGCCGTTTTCCCGCACAAAATTCGCCAGATTCACTTGTTCCAAACATTGATAAATGACTTCGTCGGAGGCATTTGATAACACCATTAGCATATTTTcc                                                                                                                                                                                                                                    >  1:711080/1‑217 (MQ=255)                                                          atatAGACATCAGCGTTATGCAAAAGGGCGCGGGCAAGCGCTAAACGTTGAATTTGCCCGCCGGACAAATTGCTTCCGCGACTGAGCAACGGCATCTCCAAACCGCCGTTTTCCCGCACAAAATTCGCCAGATTCACTTGTTCCAAACATTGATAAATGACTTCGTCGGAGGCATTTGATAACACCATTAGCATATTTTCCCGCAAGCTGCCTTTGAAAATGTAACTGCTGTGACTTACCAGCGAAAtttg                                                                                                                                                                                  >  1:393854/1‑248 (MQ=255)                                                          atatAGACATCAGCGTTATGCAAAAGGGCGCGGGCAAGCGCTAAACGTTGAATTTGCCCGCCGGACAAATTGCTTCCGCGACTGAGCAACGGCATCTCCAAACCGCCGTTTTCCCGCACAAAATTCGCCAGATTCACTTGTTCCAAACATTGATAAATGACTTCGTCGGAGGCATTTGATAACACCATTAGCATATTTTCCCGCAAGCTGCCTTTGAAAATGTAACTGCTGTGACTTACCAGCGAAAtttg                                                                                                                                                                                  >  1:539721/1‑248 (MQ=255)                                                          atatAGACATCAGCGTTATGCAAAAGGGCGCGGGCAAGCGCTAAACGTTGAATTTGCCCGCCGGACAAATTGCTTCCGCGACTGAGCAACGGCATCTCCAAACCGCCGTTTTCCCGCACAAAATTCGCCAGATTCACTTGTTCCAAACATTGATAAATGACTTCGTCGGAGGCATTTGATAACACCATTAGCATATTTTCCCGCAAGCTGCCTTTGAAAATGTAACTGCTGTGACTTACCAGCGAAAtttg                                                                                                                                                                                  >  1:762578/1‑248 (MQ=255)                                                            ataGACATCAGCGTTATGCAAAAGGGCGCGGGCAAGCGCTAAACGTTGAATTTGCCCGCCGGACAAATTGCTTCCGCGACTGAGCAACGGCATCTCCAAACCGCCGTTTTCCCGCACAAAATTCGCCAGATTCACTTGTTCCAAACATTGATAAATGACTTCGTCGGAGACATTTGATAACACCATTAGCATATTTTCCCGCAAGCTGCCTTTGAAAATGTAACTGCTGTGACTTACCAGCGAAATTTgg                                                                                                                                                                                 >  1:438633/1‑250 (MQ=255)                                                                 cATCAGCGTTATGCAAAAGGGCGCGGGCAAGCGCTAAACGTTGAATTTGCCCGCCGGACAAATTGCTTCCGCGACTGAGCAACGGCATCTCCAAACCGCCGTTTTCCCGCACAAAATTCGCCAGATTCACTTGTTCCAAACATTGATAAATGACTTCGTCGGAGGCATTTGATAACACCATTAGCATATTTTCCCGCAAGCTGCCTTTGAAAATGTAACTGCTGTGACTTACCAGCGAAATTTGGCGatat                                                                                                                                                                           >  1:782960/1‑251 (MQ=255)                                                                      gCGTTATGCAAAAGGGCGCGGGCAAGCGCTAAACGTTGAATTTGCCCGCCGGACAAATTGCTTCCGCGACTGAGCAACGGCATCTCCAAACCGCCGTTTTCCCGCACAAAATTCGCCAGATTCACTTGTTCCAAACATTGATAAATGACTTCGTCGGAGGCATTTGATAACACCATTAGCATATTTTCCCGCAAGCTGCCTTTGAAAATGTAAc                                                                                                                                                                                                           <  1:679187/214‑1 (MQ=255)                                                                      gCGTTATGCAAAAGGGCGCGGGCAAGCGCTAAACGTTGAATTTGCCCGCCGGACAAATTGCTTCCGCGACTGAGCAACGGCATCTCCAAACCGCCGTTTTCCCGCACAAAATTCGCCAGATTCACTTGTTCCAAACATTGATAAATGACTTCGTCGGAGGCATTTGATAACACCATTAGCATATTTTCCCGCAAGCTGCCTTTGAAAATGTAACTGCTGTGACTTACCAGCGAAATTTGGCGATATAAGGa                                                                                                                                                                      >  1:237166/1‑251 (MQ=255)                                                                       cGTTATGCAAAAGGGCGCGGGCAAGCGCTAAGCGTTGAATTTGCCCGCCGGACAAATTGCTTCCGCGACTGAGCAACGGCATCTCCAAACCGCCGTTTTCCCGCACAAAATTCGCCAGATTCACTTGTTCCAAACATTGATAAATGACTTCGTCGGAGGCATTTGATAACACCATTAGCATATTTTCCCGCAAGCTGCCTTTGAAAATGTAACTGCTGTGACTTACCAGCGAAATTTGGCGATATAAGGa                                                                                                                                                                      <  1:338501/250‑1 (MQ=255)                                                                       cGTTATGCAAAAGGGCGCGGGCAAGCGCTAAACGTTGAATTTGCCCGCCGGACAAATTGCTTCCGCGACTGAGCAACGGCATCTCCAAACCGCCGTTTTCCCGCACAAAATTCGCCAGATTCACTTGTTCCAAACATTGATAAATGACTTCGTCGGAGGCATTTGATAACACCATTAGCATATTTTCCCGCAAGCTGCCTTTGAAAATGTAACTGCTGTGACTTACCAGCGAAATTTGGCGATATAAgg                                                                                                                                                                       <  1:695526/249‑1 (MQ=255)                                                                       cGTTATGCAAAAGGGCGCGGGCAAGCGCTAAACGTTGAATTTGCCCGCCGGACAAATTGCTTCCGCGACTGAGCAACGGCATCTCCAAACCGCCGTTTTCCCGCACAAAATTCGCCAGATTCACTTGTTCCAAACATTGATAAATGACTTCGTCGGAGGCATTTGATAACACCATTAGCATATTTTCCCGCAAGCTGCCTTTGAAAATGTAACTGCTGTGACTTACCAGCGAAATTTGGCGATATAAGGa                                                                                                                                                                      <  1:449114/250‑1 (MQ=255)                                                                       cGTTATGCAAAAGGGCGCGGGCAAGCGCTAAACGTTGAATTTGCCCGCCGGACAAATTGCTTCCGCGACTGAGCAACGGCATCTCCAAACCGCCGTTTTCCCGCACAAAATTCGCCAGATTCACTTGTTCCAAACATTGATAAATGACTTCGTCGGAGGCATTTGATAACACCATTAGCATATTTTCCCGCAAGCTGCCTTTGAAAATGTAACTGCTGTGACTTACCAGCGAAATTTGGCGATATAAGGa                                                                                                                                                                      <  1:708949/250‑1 (MQ=255)                                                                          tATGCAAAAGGGCGCGGGCAAGCGCTAAACGTTGAATTTGCCCGCCGGACAAATTGCTTCCGCGACTGAGCAACGGCATCTCCAAACCGCCGTTTTCCCGCACAAAATTCGCCAGATTCACTTGTTCCAAACATTGATAAATGACTTCGTCGGAGGCATTTGATAACACCATTAGCATATTTTCCCGCAAGCTGCCTTTGAAAATGTAACTGCTGTGACTTACCAGCGAAATTTGGCGATATAAGGAATgg                                                                                                                                                                  <  1:893511/251‑1 (MQ=255)                                                                           aTGCAAAGGGGCGCGGGCAAGCGCTAAACGTTGAATTTGCCCGCCGGACAAATTGCTTCCGCGACTGAGCAACGGCATCTCCAAACCGCCGTTTTCCCGCACAAAATTCGCCAGATTCACTTGTTCCAAACATTGATAAATGACTTCGTCGGAGGCATTTGATAACACCATTAGCATATTTTCCCGCAAGCTGCCTTTGAAAATGTAACTGCTGTGa                                                                                                                                                                                                   <  1:789930/217‑1 (MQ=255)                                                                           aTGCAAAAGGGCGCGGGCAAGCGCTAAACGTTGAATTTGCCCGCGGGACAAATTGCTTCCGCGACTGAGCGACGGCATCTCCAAACCGCCGTTTTCCCGCACAAAATTCGCCAGATTCACTTGTTCCAAACATTGATAAATGACTTCGTCGGAGGCATTTGATAACACCATTAGCATATTTTCCCGCAAGCTGCCTTTGAAAATGTAACTGCTGTGa                                                                                                                                                                                                   <  1:837155/217‑1 (MQ=255)                                                                           aTGCAAAAGGGCGCGGGCAAGCGCTAAACGTTGAATTTGCCCGCCGGACAAATTGCTTCCGCGACTGAGCAACGGTATCTCCAAACCGCCGTTTTCCCGCACAAAATTCGCCAGATTCACTTGTTCCAAACATTGATAAATGACTTCGTCGGAGGCATTTGATAACACCATTAGCATATTTTCCCGCAAGCTGCCTTTGAAAATGTAACTGCTGTGACTTACCAGCGAAATTTGGCGATATAAgg                                                                                                                                                                       <  1:432241/245‑1 (MQ=255)                                                                           aTGCAAAAGGGCGCGGGCAAGCGCTAAACGTTGAATTTGCCCGCCGGACAAATTGCTTCCGCGACTGAGCAACGGCATCTCCAAACCGCCGTTTTCCCGCACAAAATTCGCCAGATTCACTTGTTCCAAACATTGATAAATGACTTCGTCGGAGGCATTTGATAACACCATTAGCATATTTTcc                                                                                                                                                                                                                                    <  1:666814/184‑1 (MQ=255)                                                                           aTGCAAAAGGGCGCGGGCAAGCGCTAAACGTTGAATTTGCCCGCCGGACAAATTGCTTCCGCGACTGAGCAACGGCATCTCCAAACCGCCGTTTTCCCGCACAAAATTCGCCAGATTCACTTGTTCCAAACATTGATAAATGACTTCGTCGGAGGCATTTGATAACACCATTAGCATATTTTCCCGCAAGCTGCCTTTGAAAATGTAActgctg                                                                                                                                                                                                      <  1:652900/214‑1 (MQ=255)                                                                           aTGCAAAAGGGCGCGGGCAAGCGCTAAACGTTGAATTTGCCCGCCGGACAAATTGCTTCCGCGACTGAGCAACGGCATCTCCAAACCGCCGTTTTCCCGCACAAAATTCGCCAGATTCACTTGTTCCAAACATTGATAAATGACTTCGTCGGAGGCATTTGATAACACCATTAGCATATTTTCCCGCAAGCTGCCTTTGAAAATGTAACTGCTGTGACTTACCAGCGAAATTTGGCGATATAAGGAATgg                                                                                                                                                                  >  1:111644/1‑250 (MQ=255)                                                                           aTGCAAAAGGGCGCGGGCAAGCGCTAAACGTTGAATTTGCCCGCCGGACAAATTGCTTCCGCGACTGAGCAACGGCATCTCCAAACCGCCGTTTTCCCGCACAAAATTCGCCAGATTCACTTGTTCCAAACATTGATAAATGACTTCGTCGGAGGCATTTGATAACACCATTAGCATATTTTCCCGCAAGCTGCCTTTGAAAATGTAACTGCTGTGACTTACCAGCGAAATTTGGCGATATAAGGAATgg                                                                                                                                                                  >  1:581391/1‑250 (MQ=255)                                                                           aTGCAAAAGGGCGCGGGCAAGCGCTAAACGTTGAATTTGCCCGCCGGACAAATTGCTTCCGCGACTGAGCAACGGCATCTCCAAACCGCCGTTTTCCCGCACAAAATTCGCCAGATTCACTTGTTCCAAACATTGATAAATGACTTCGTCGGAGGCATTTGATAACACCATTAGCATATTTTCCCGCAAGCTGCCTTTGAAAATGTAACTGCTGTGACTTACCAGCGAAATTTGGCGATATAAGGAATgg                                                                                                                                                                  >  1:661713/1‑250 (MQ=255)                                                                             gCAAAAGGGCGCGGGCAAGCGCTAAACGTTGAATTTGCCCGCCGGACAAATTGCTTCCGCGACTGAGCAACGGCATCTCCAAACCGCCGTTTTCCCGCACAAAATTCGCCAGATTCACTTGTTCCAAACATTGATAAATGACTTCGTCGGAGGCATTTGATAACACCATTAGCATATTTTCCCGCAAGCTGCCTTTGAAAATGTAACTGCTGTGACTTACCAGCGAAATTTGGCGATATAAGGAATGGCgg                                                                                                                                                               >  1:535369/1‑251 (MQ=255)                                                                              cAAAAGGGCGCGGGCAAGCGCTAAACGTTGAATTTGCCCGCCGGACAAATTGCTTCCGCGACTGAGCAACGGCATCTCCAAACCGCCGTTTTCCCGCACAAAATTCGCCAGATTCACTTGTTCCAAACATTGATAAATGACTTCGTCGGAGGCATTTGATAACACCATTAGCATATTTTCCCGCAAGCTGCCTTTGAAAATGTAACTGCTGTGACTTACCAGCGAAATTTGGCGATATAAGGAATGGCgg                                                                                                                                                               <  1:216007/250‑1 (MQ=255)                                                                                             aaGCGTTAATCGTTGAATTTGCCCGCCGGCAAAATTGCTTCCGCGGATGAGCGTCGGCTTCTCCAAGCCGCCGTTTTCCCGCACAAAATTCGCCAGATTCACTTGTTCCAAACATTGATAAATGACTTCGTCGGAGGCATTTGATAACTCCATTAGCATAGTTTCCCGCAAGCTGGCTTTGAAAATGTAACTGCTGTGACTTACCAGCGAAATTTGGGGATATAAGGAATGGCGGTTAATCTGTTTAatat                                                                                                                                               <  1:263113/251‑1 (MQ=255)                                                                                             aaGCGCTAACCGTTGAATTTGCCCGCCGGACAAATTGCTTCCGCGACTGAGCAACGGCATCTCCAAACCGCCGTTTTCCCGCACAAAATTCGCCAGATTCACTTGTTCCAAACATTGATAAATGACTTCGTCGGAGGCATTTGATAACACCATTAGCATATTTTCCCGCAAGCTGCCTTTGAAAATGTAACTGCTGTGACTTACCAGCGAAATTTGGCGATATAAGGAATGGCGGTTAATCTGTTTAatat                                                                                                                                               <  1:795849/251‑1 (MQ=255)                                                                                             aaGCGCTAAACGTTGAATTTGCCCGCCGGACAAATTGCTTCCGCGACTGAGCAACGGCATCTCCAAACCGCCGTTTTCCCGCACAAAATTCGCCAGATTCACTTGTTCCAAACATTGATAAATGACTTCGTCGGAGGCATTTGATAACGCCATTAGCATATTTTCCCGCAAGCTGCCTTTGAAAATGTAACTGCTGTGACTTACCAGCGAAATTTGGCGATATAAGGAATGGCGGTTAATCTGTTTAatat                                                                                                                                               <  1:347158/251‑1 (MQ=255)                                                                                             aaGCGCTAAACGTTGAATTTGCCCGCCGGACAAATTGCTTCCGCGACTGAGCAACGGCATCTCCAAACCGCCGTTTTCCCGCACAAAATTCGCCAGATTCACTTGTTCCAAACATTGATAAATGACTTCGTCGGAGGCATTTGATAACACCATTAGCATATTTTCCCGCAAGCTGCCTTTGAAAATGTAACTGCTGTGACTTACCAGCGAAATTTGGCGATATAAGGAATGGCGGTTAATCTGTTTAatat                                                                                                                                               <  1:45191/251‑1 (MQ=255)                                                                                             aaGCGCTAAACGTTGAATTTGCCCGCCGGACAAATTGCTTCCGCGACTGAGCAACGGCATCTCCAAACCGCCGTTTTCCCGCACAAAATTCGCCAGATTCACTTGTTCCAAACATTGATAAATGACTTCGTCGGAGGCATTTGATAACACCATTAGCATATTTTCCCGCAAGCTGCCTTTGAAAATGTAACTGCTGTGACTTACCAGCGAAATTTGGCGATATAAGGAATGGCGGTTAATCTGTTTAatat                                                                                                                                               <  1:211263/251‑1 (MQ=255)                                                                                             aaGCGCTAAACGTTGAATTTGCCCGCCGGACAAATTGCTTCCGCGACTGAGCAACGGCATCTCCAAACCGCCGTTTTCCCGCACAAAATTCGCCAGATTCACTTGTTCCAAACATTGATAAATGACTTCGTCGGAGGCATTTGATAACACCATTAGCATATTTTCCCGCAAGCTGCCTTTGAAAATGTAACTGCTGTGACTTACCAGCGAAATTTGGCGATATAAGGAATGGCGGTTAATCTGTTTAatat                                                                                                                                               <  1:696818/251‑1 (MQ=255)                                                                                                   tAAACGTTGAATTTGCCCGCCGGACAAATTGCTTCCGCGACTGAGCAACGGCATCTCCAAACCGCCGTTTTCCCGCACAAAATTCGCCAGATTCACTTGTTCCAAACATTGATAAATGACTTCGTCGGAGGCATTTGATAACACCATTAGCATATTTTCCCGCAAGCTGCCTTTGAAAATGTAACTGCTGTGACTTACCAGCGAAATTTGGCGATATAAGGAATGGCGGTTAATCTGTTTAATATCGATGc                                                                                                                                         <  1:333931/251‑1 (MQ=255)                                                                                                       cGTTGAATTTGCCCGCCGGACAAATTGCTTCCGCGACTGAGCAACGGCATCTCCAAACCGCCGTTTTCCCGCACAAAATTCGCCAGATTCACTTGTTCCAAACATTGATAAATGACTTCGTCGGAGGCATTTGATAACACCATTAGCATATTTTCCCGCAAGCTGCCTTTGAAAATGTAACTGCTGTGACTTACCAGCGAAATTTGGCGATATAAGGAATGGCGGTTAATc                                                                                                                                                         <  1:336250/231‑1 (MQ=255)                                                                                                       cGTTGAATTTGCCCGCCGGACAAATTGCTTCCGCGACTGAGCAACGGCATCTCCAAACCGCCGTTTTCCCGCACAAAATTCGCCAGATTCACTTGTTCCAAACATTGATAAATGACTTCGTCGGAGGCATTTGATAACACCATTAGCATATTTTCCCGCAAGCTGCCTTTGAAAATGTAACTGCTGTGACTTACCAGCGAAATTTGGCGATATAAGGAATGGCGGTTAATCTGTTTAatat                                                                                                                                               <  1:415274/241‑1 (MQ=255)                                                                                                            aaTTTGCCCGCCGGACAAATTGCTTCCGCGACTGAGCAACGGCATCTCCAAACCGCCGTTTTCCCGCACAAAATTCGCCAGATTCACTTGTTCCAAACATTGATAAATGACTTCGTCGGAGGCATTTGATAACACCATTAGCATATTTTCCCGCAAGCTGCCTTTGAAAATGTAACTGCTGTGACTTACCAGCGAAATTTGGCGATATAAGGAATGGCGGTTAATCTGTTTAATATCGATGCCGTTAAAc                                                                                                                                 <  1:742587/250‑1 (MQ=255)                                                                                                                                          aCTGAGCAACGGCATCTCCAAACCGCCGTTTTCCCGCACAAAATTCGCCAGATTCACTTGTTCCAAACATTGATAAATGACTTCGTCGGAGGCATTTGATAACACCATTAGCATATTTTCCCGCAAGCTGCCTTTGAAAATGTAACTGCTGTGACTTACCAGCGAAATTTGGCGATATAAGGAATGGCGGTTAATCTGTTTAATATCGATGCCGTTAAACAAAATTTCGCCTTGTTGCGCCTGATAGAAc                                                                                                   <  1:392719/250‑1 (MQ=255)                                                                                                                                           cTGAGCAACGGCATCTCCAAACCGCCGTTTTCCCGCACAAAATTCGCCAGATTCACTTGTTCCAAACATTGATAAATGACTTCGTCGGAGGCATTTGATAACACCATTAGCATATTTTCCCGCAAGCTGCCTTTGAAAATGTAACTGCTGTGACTTACCAGCGAAATTTGGCGATATAAGGAATGGCGGTTAATCTGTTTAATATCGATGCCGTTAAACAAAATTTCGCCTTGTTGCGCCTGATAGAAccc                                                                                                 <  1:680915/251‑1 (MQ=255)                                                                                                                                                    ggCATCTCCAAACCGCCGTTTTCCCGCACAAAATTCGCCAGATTCACTTGTTCCAAACATTGATAAATGACTTCGTCGGAGGCATTTGATAACACCATTAg                                                                                                                                                                                                                                              >  1:429083/1‑101 (MQ=255)                                                                                                                                                     gCATCTCCAAACCGCCGTTTTCCCGCACAAAATTCGCCAGATTCACTTGTTCCAAACATTGATAAATGACTTCGTCGGAGGCATTTGATAACACCATTAGCATATTTTCCCGCAAGCTGCCTTTGAAAATGTAACTGCTGTGACTTACCAGCGAAATTTGGCGATATAAGGAAt                                                                                                                                                                    >  1:516381/1‑174 (MQ=255)                                                                                                                                                      cATCTCCAAACCGCCGTTTTCCCGCACAAAATTCGCCAGATTCACTTGTTCCAAACATTGATAAATGACTTCGTCGGAGGCATTTGATAACACCATTAGCATATTTTCCCGCAAGCTGCCTTTGAAAATGTAACTGCTGTGACTTACCAGCGAAATTTGGCGATATAAGGAATGGCGGTTAATCTGTTTAATATCGATGCCGTTAAACAAAATTTCGCCTTGTTGCGCCTGATAGAACCCCATTAGTAAAg                                                                                      <  1:162948/251‑1 (MQ=255)                                                                                                                                                      cATCTCCAAACCGCCGTTTTCCCGCACAAAATTCGCCAGATTCACTTGTTCCAAACATTGATAAATGACTTCGTCGGAGGCATTTGATAACACCATTAGCATATTTTCCCGCAAGCTGCCTTTGAAAATGTAACTGCTGTGACTTACCAGCGAAATTTGGCGATATAAGGAATGGCGGTTAATCTGTTTAATATCGATGCCGTTAAACAAAATTTCGCCTTGTTGCGCCTGATAGAACCCCATTAGTAAAg                                                                                      <  1:549906/251‑1 (MQ=255)                                                                                                                                                        tctcCAAACCGCCGTTTTCCCGCACAAAATTCGCCAGATTCACTTGTTCCAAACATTGATAAATGACTTCGTCGGAGGCATTTGATAACACCATTAGCATATTTTCCCGCAAGCTGCCTTTGAAAATGTAACTGCTGTGACTTACCAGCGAAATTTGGCGATATAAGGAATGGCGGTTAATCTGTTTAATATCGATGCCGTTAAACAAAATTTCGCCTTGTTGCGCCTGATAGAACCCCATTAGTAAAGAt                                                                                    >  1:732553/1‑251 (MQ=255)                                                                                                                                                         ctcCAAACCGCCGTTTTCCCGCACAAAATTCGCCAGATTCACTTGTTCCAAACATTGATAAATGACTTCGTCGGAGGCATTTGATAACACCATTAGCATATTTTCCCGCAAGCTGCCTTTGAAAATGTAACTGCTGTGACTTACCAGCGAAATTTGGCGATATAAGGAATGGCGGTTAATCTGTTTAatat                                                                                                                                               <  1:724529/191‑1 (MQ=255)                                                                                                                                                         ctcCAAACCGCCGTTTTCCCGCACAAAATTCGCCAGATTCACTTGTTCCAAACATTGATAAATGACTTCGTCGGAGGCATTTGATAACACCATTAGCATATTTTCCCGCAAGCTGCCTTTGAAAATGTAACTGCTGTGACTTACCAGCGAAATTTGGCGATATAAGGAATGGCGGTTAATCTGTTTAATATCGATGCCGTTAAACAAAATTTCGCCTTGTTGCGCCTGATAGAACCCCATTAGTAAAGATa                                                                                   >  1:184770/1‑251 (MQ=255)                                                                                                                                                                  gccgTTTTCCCGCACAAAATTCGCCAGATTCACTTGTTCCAAACATTGATAAATGACTTCGTCGGAGGCATTTGATAACACCATTAGCATATTTTCCCGCAAGCTGCCTTTGAAAATGTAACTGCTGTGACTTACCAGCGAAATTTGGCGATATAAGGAATGGCGGTTAATCTGTTTAATATCGATGCCGTTAAACAAAATTTCGCCTTGTTGCGCCTGATAGAACCCCATTAGTaa                                                                                        <  1:96885/237‑1 (MQ=255)                                                                                                                                                                        ttctcGCACAAAATTCGCCAGATTCACTTGTTCCAAACATTGATAAATGACTTCGTCGGAGGCATTTGATAACACCATTAGCATATTTTCCCGCAAGCTGCCTTTGAAAATGTAACTGCTGTGACTTACCAGCGAAATTTGGCGATATAAGGAATGGCGGTTAATCTGTTTAatat                                                                                                                                               >  1:252301/5‑176 (MQ=255)                                                                                                                                                                        ttCCCGCACAAAATTCGCCAGATTCACTTGTTCCAAACATTGATAAATGACTTCGTCGGAGGCATTTGATAACACCATTAGCATATTTTCCCGCAAGCTGCCTTTGAAAATGTAACTGCTGTGACTTACCAGCGAAATTTGGCGATATAAGGAATGGCGGTTAATCTGTTTAATATCGATGCCg                                                                                                                                       <  1:472549/184‑1 (MQ=255)                                                                                                                                                                                  aaaTTCGCCAGATTCACTTGTTCCAAACATTGATAAATGACTTCGTCGGAGGCATTTGATAACACCATTAGCATATTTTCCCGCAAGCTGCCTTTGAAAATGTAACTGCTGTGACTTACCAGCGAAATTTGGCGATATAAGGAATGGCGGTTAATCTGTTTAATATCGATGCCGTTAAACAAAATTTCGCCTTGTTGCGCCTGATAGAACCCCATTAGTAAAGATAc                                                                                  >  1:572667/1‑227 (MQ=255)                                                                                                                                                                                   aaTTCGCCAGATTCACTTGTTCCAAACATTGATAAATGACTTCGTCGGAGGCATTTGATAACACCATTAGCATATTTTCCCGCAAGCTGCCTTTGAAAATGTAACTGCTGTGACTTACCAGCGAAATTTGGCGATATAAGGAATGGCGGTTAATCTGTTTAATATCGATGCCGTTAAACAAAATTTCGCCTTGTTGCGCCTGATAGAACCCCATTAGTAAAGATACCAAGGTCGATTTGCCACAACCGCtt                                                         <  1:81513/251‑1 (MQ=255)                                                                                                                                                                                         ccAGATTCACTTGTTCCAAACATTGATAAATGACTTCGTCGGAGGCATTTGATAACACCATTAGCATATTTTCCCGCAAGCTGCCTTTGAAAATGTAACTGCTGTGACTTACCAGCGAAATTTGGCGATATAAGGAATGGCGGTTAATCTGTTTAATATCGATGCCGTTAAACAAAATTTCGCCTTGTTGCGCCTGATAGAACCCCATTAGTAAAGATACCAAGGTCGATTTGCCACAACCGCTTTTGccc                                                   <  1:551840/251‑1 (MQ=255)                                                                                                                                                                                             aTTCACTTGTTCCAAACATTGATAAATGACTTCGTCGGAGGCATTTGATAACACCATTAGCATATTTTCCCGCAAGCTGCCTTTGAAAATGTAACTGCTGTGACTTACCAGCGAAATTTGGCGATATAAGGAATGGCGGTTAATCTGTTTAATATCGATGCCGTTAAACAAAATTTCGCCTTGTTGCGCCTGATAGAACCCCATTAGTAAAGATACCAAGGt                                                                            >  1:893997/1‑222 (MQ=255)                                                                                                                                                                                                     gTTCCAAACATTGATAAATGACTTCGTCGGAGGCATTTGATAACACCATTAGCATATTTTCCCGCAAGCTGCCTTTGAAAATGTAACTGCTGTGACTTACCAGCGAAATTTGGCGATATAAGGAATGGCGGTTAATc                                                                                                                                                         >  1:781729/1‑137 (MQ=255)                                                                                                                                                                                                     gTTCCAAACATTGATAAATGACTTCGTCGGAGGCATTTGATAACACCATTAGCATATTTTCCCGCAAGCTGCCTTTGAAAATGTAACTGCTGTGACTTACCAGCGAAATTTGGCGATATAAGGAATGGCGGTTAATCTGTTTAATATCGATGCCGTTAAACAAAATTTCGCCTTGTTGCGCCTGATAGAACCCCATTAGTaa                                                                                        <  1:731796/202‑1 (MQ=255)                                                                                                                                                                                                       tCCAAACATTGATAAATGACTTCGTCGGAGGCATTTGATAACACCATTAGCATATTTTCCCGCAAGCTGCCTTTGAAAATGTAACTGCTGTGACTTACCAGCGAAATTTGGCGATATAAGGAATGGCGGTTAATCTGTTTAATATCGATGCCGTTAAACAAAATTTCGCCTTGTTGCGCCTGATAGAACCCCATTAGTAAAg                                                                                      >  1:688804/1‑202 (MQ=255)                                                                                                                                                                                                                 gATAAATGACTTCGTCGGAGGCATTTGATAACACCATTAGCATATTTTCCCGCAAGCTGCCTTTGAAAATGTAACTGCTGTGACTTACCAGCGAAATTTGGCGATATAAGGAATGGCGGTTAATCTGTTTAATATCGATGCCGTTAAACAAAATTTTGCCTTGTTGCGCCTGATAGAACCCCATTAGTAAAGATACCAAGGTCGATTTGCCACAACCGCttt                                                        >  1:359428/1‑222 (MQ=255)                                                                                                                                                                                                                  aTAAATGACTTCGTCGGAGGCATTTGATAACACCATTAGCATATTTTCCCGCAAGCTGCCTTTGAAAATGTAACTGCTGTGACTTACCAGCGAAATTTGGCGATATAAGGAATGGCGGTTAATCTGTTTAATATCGATGCCGTTAAACAAAATTTCGCCTTGTTGCGCCTGATAGAACCCCATTAGTAAAGATACCAAGGTCGATTTTCCACAACCGCTTTTGCCCACGAAAACCGTTAACTGTTTctg                            >  1:387173/1‑247 (MQ=255)                                                                                                                                                                                                                          cTTCGTCGGAGGCATTTGATAACACCATTAGCATATTTTCCCGCAAGCTGCCTTTGAAAATGTAACTGCTGTGACTTACCAGCGAAATTTGGCGATATAAGGAATGGCGGTTAATCTGTTTAatat                                                                                                                                               <  1:782670/126‑1 (MQ=255)                                                                                                                                                                                                                             cgtcgGAGGCATTTGATAACACCATTAGCATATTTTCCCGCAAGCTGCCTTTGAAAATGTAACTGCTGTGACTTACCAGCGAAATTTGGCGATATAAGGAATGGCGGTTAATCTGTTTAATATCGATGCCGTTAAACAAAATTTCGCCTTGTTGCGCCTGATAGAACCCCATTAGTAAAGATACCAAGGTCGATTTGCCACAACCGCTTTTGCCCACGAAAACCGTTAACTGTTTCGGCTGGATGCTGAgg               <  1:360123/251‑1 (MQ=255)                                                                                                                                                                                                                             cgtcgGAGGCATTTGATAACACCATTAGCATATTTTCCCGCAAGCTGCCTTTGAAAATGTAACTGCTGTGACTTACCAGCGAAATTTGGCGATATAAGGAATGGCGGTTAATCTGGTTAATATCGATGCCGTTAAACAAAATTTCCCCTTGTTGCGCCTGATAGAACCCCATTAGTAAAGATACCAAg                                                                              >  1:228601/1‑188 (MQ=255)                                                                                                                                                                                                                                      cATTTGATAACACCATTAGCATATTTTCCCGCAAGCTGCCTTTGAAAATGTAACTGCTGTGACTTACCAGCGAAATTTGGCGATATAAGGAATGGCGGTTAATCTGTTTAATATCGATGCCGTTAAACAAAATTTCGCCTTGTTGCGCCTGATAGAACCCCATTAGTAAAGATACCAAGGTCGATTTGCCACAACCGCTTTTGCCCACGAAAACCGTTAACTGTTTCGGCTGGATGCTGAGGGTTAAGcc       <  1:616928/250‑1 (MQ=255)                                                                                                                                                                                                                                       aTTTGATAACACCATTAGCATATTTTCCCGCAAGCTGCCTTTGAAAATGTAACTGCTGTGACTTACCAGCGAAATTTGGCGATATAGGGAATGGCGGTTAATCTGTTTAATATCGATGCCGTTAAACAAAATTTCGCCTTGTTGCGCCTGATAGAACCCCATTAGTAAAGATACCAAGGTCGATTTGCCACAACCGCTTTTGCCCACGAAAACCGTTAACTGTTTCGGCTGGATGCTGAGGGTTAAGc        >  1:546808/1‑248 (MQ=255)                                                                                                                                                                                                                                        tttGATAACACCATTAGCATATTTTCCCGCAAGCTGCCTTTGAAAATGTAACTGCTGTGACTTACCAGCGAAATTTGGCGATATAAGGAATGGCGGTTAATCTGTTTAATATCGATGCCGTTAAACAAAATTTCGCCTTGTTGCGCCTGATAGAACCCCATTAGTAAAGATACCAAGGTCGATTTGCCACAACCGCTTTTGCCCACGAAAACCGTTAACTGTTTCGGCTGGATGCTGAGGGTTAAGCCGtt    >  1:220155/1‑251 (MQ=255)                                                                                                                                                                                                                                          tGATAACACCATTAGCATATTTTCCCGCAAGCTGCCTTTGAAAATGTAACTGCTGTGACTTACCAGCGAAATTTGGCGATATAAGGAATGGCGGTTAATCTGTTTAATATCGATGCCGTTAAACAAAATTTTGCCTTGTTGCGCCTGATAGAACCCCATTAGGAAAGATACCAAGGTCGATTTTCCACAACCGCTTTTGCCCACCAAAACCCTTAACTGTTTCGGCTGGATGCTGAGGGTTAAGCCGTTaa  >  1:503245/1‑251 (MQ=255)                                                                                                                                                                                                                                          tGATAACACCATTAGCATATTTTCCCGCAAGCTGCCTTTGAAAATGTAACTGCTGTGACTTACCAGCGAAATTTGGCGATATAAGGAATGGCGGTTAATCTGTTTAATATCGATGCCGTTAAACAAAATTTCGCCTTGTTGCGCCTGATAGAACCCCATTAGTAAAGATACCAAGGTCGATTTGCCACAACCGCTTTTGCCCACGAAAACCGTTAACt                                   <  1:355211/218‑1 (MQ=255)                                                                                                                                                                                                                                          tGATAACACCATTAGCATATTTTCCCGCAAGCTGCCTTTGAAAATGTAACTGCTGTGACTTACCAGCGAAATTTGGCGATATAAGGAATGGCGGTTAATCTGTTTAATATCGATGCCGTTAAACAAAATTTCGCCTTGTTGCGCCTGATAGAACCCCATTAGTAAAGATACCAAGGTCGATTTGCCACAACCGCTTTTGCCCACGAAAACCGTTAACTGTTTCGGCTGGATGCTGAGGGTTAAGCCGTTaa  >  1:343401/1‑251 (MQ=255)                                                                                                                                                                                                                                          tGATAACACCATTAGCATATTTTCCCGCAAGCTGCCTTTGAAAATGTAACTGCTGTGACTTACCAGCGAAATTTGGCGATATAAGGAATGGCGGTTAATCTGTTTAATATCGATGCCGTTAAACAAAATTTCGCCTTGTTGCGCCTGATAGAACCCCATTAGTAAAGATACCAAGGTCGATTTGCCACAACCGCTTTTGCCCACGAAAACCGTTAACTGTTTCGGCTGGATGCTGAGGGTTAAGCCGTTaa  >  1:290315/1‑251 (MQ=255)                                                                                                                                                                                                                                          tGATAACACCATTAGCATATTTTCCCGCAAGCTGCCTTTGAAAATGTAACTGCTGTGACTTACCAGCGAAATTTGGCGATATAAGGAATGGCGGTTAATCTGTTTAATATCGATGCCGTTAAACAAAATTTCGCCTTGTTGCGCCTGATAGAACCCCATTAGTAAAGATACCAAGGTCGATTTGCCACAACCGCTTTTGCCCACGAAAACCGTTAACTGTTTCGGCTGGATGCTGAGGGGTAAGCCGTTaa  >  1:60444/1‑251 (MQ=255)                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                   AATTGCAGAATAATTTCTTCGCTTTCTACATCGATGTTACTGGTAGCTTCATCGAAAATATAGACATCAGCGTTATGCAAAAGGGCGCGGGCAAGCGCTAAACGTTGAATTTGCCCGCCGGACAAATTGCTTCCGCGACTGAGCAACGGCATCTCCAAACCGCCGTTTTCCCGCACAAAATTCGCCAGATTCACTTGTTCCAAACATTGATAAATGACTTCGTCGGAGGCATTTGATAACATCATTAGCATATTTTCCCGCAAGCTGCCTTTGAAAATGTAACTGCTGTGACTTACCAGCGAAATGTGGCGATATAAGGAATGGCGGTTAATCTGTTTAATATCGATGCCGTTAAACAAAATTTCGCCTTGTTGCGCCTGATAGAACCCCATTAGTAAAGATACCAAGGTCGATTTGCCACAACCGCTTTTGCCCACGAAAACCGTTAACTGTTTCGGCTGGATGCTGAGGGTTAAGCCGTTAA  >  1/468694‑469177

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑