BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	462,227	A→G	H75H (CAT→CAC)	unknown ←	tRNA 5‑methylaminomethyl‑2‑thiouridine synthase subunit TusD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	462,227	0	A	G	100.0%	394.0 / NA	130	H75H (CAT→CAC)	unknown	tRNA 5‑methylaminomethyl‑2‑thiouridine synthase subunit TusD
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (66/64); total (66/64)

Only 100 of 130 total aligned reads displayed.

CGCAAAATGCCGTGGCGGCAAGCAGCGCGTCTAATCCTTCGCGTGTATTTGCCGAACCATAAGGCGCGTGACGGAAAATAAAGGCAAGTTTACTCATAGGGTTATCACCCGATCGGCTTGTAATAAGGCTTGGCTGAA‑TTCACCCAAGCCGGCAAGTTGAAAAACGTCCGCCAGGTTAACCGCACTTTTATCGGCGGCAGAATAGCTATCCACCACGCCACGCCGTTGAGCGGCGGCAACGCACAAATGCAGCGGCACGTTAAATTGACGGGAAAATGCCTGCCAGGCTTGGACCAAATTAAATTCGTCATTAGCGGGATAAACCAAGGCGTTGCCGTTGGTTACGCCATCCTGATAAAAAAAGATTTGGCTGATATGATGCTTTTGCACCAGTACTTGTGCCAGTTGGTACGCCAGAAACGCGCCTTGACTACCGTAAACCGGTTGTTTCACCACCAACACATAATCCATGACTTATTCATCCTGATCT  >  1/461981‑462470

cgcAAAATGCCGTGGCGGCAAGCAGCGCGTCTAATCCTTCGCGTGTATTTGCCGAGCCATAAGGCGCGTGACGGAAAATAAAGGCAAGTTTACTCATAGGGTTATCACCCGATCGGCTTGTAATAAGGCTTGGCTGAA‑TTCACCCAAGCCGGCAAGTTGAAAAACGTCCGCCAGGTTAACCGCACTTTTATCGGCGGCAGAATAGCTATCCACCACGCCACGCCGTTGAGCGGCGGCAACGCACAAGTGc                                                                                                                                                                                                                                                  <  1:222443/250‑1 (MQ=255)

  cAAAATGCCGTGGCGGCAAGCAGCGCGTCTAATCCTTCGCGTGTATTTGCCGAACCATAAGGCGCGTGACGGAAAATAAAGGCAAGTTTACTCATAGGGTTATCACCCGATCGGCTTGTAATAAGGCTTGGCTGAA‑TTCACCCAAGCCGGCAAGTTGAAAAACGTCCGCCAGGTTAACCGCACTTTTATCGGCGGCAGAATAGCTATCCACCACGCCACGCCGTTGAGCGGCGGCAACGCACAAGTGCAg                                                                                                                                                                                                                                                <  1:715698/250‑1 (MQ=255)

         ccGTGGCGGCAAGCAGCGCGTCTAATCCTTCGCGTGTATTTGCCGAACCATAAGGCGCGTGACGGAAAATAAAGGCAAGTTTACTCATAGGGTTATCACCCGATCGGCTTGTAATAAGGCTTGGCTGAA‑TTCACCCAAGCCGGCAAGTTGAAAAACGTCCGCCAGGTTAACCGCACTTTTATCGGCGGCAGAATAGCTATCCACCACGCCACGCCGTTGAGCGGCGGCAACGCACAAGTGCAGCGGCACGt                                                                                                                                                                                                                                        >  1:608978/1‑251 (MQ=255)

             ggcggcAAGCAGCGCGTCTAATCCTTCGCGTGTATTTGCCGAACCATAAGGCGCGTGACGGAAAATAAAGGCAAGTTTACTCATAGGGTTATCACCCGATCGGCTTGTAATAAGGCTTGGCTGAA‑TTCACCCAAGCCGGCAAGTTGAAAAACGTCCGCCAGGTTAACCGCACTTTTATCGGCGGCAGAATAGCTATCCACCACGCCACGCCGTTGAGCGGCGGCAACGCACAAGTGCAGCGGCACGTTaaa                                                                                                                                                                                                                                    >  1:575484/1‑251 (MQ=255)

                           cgTCTAATCCTTCGCGTGTATTTGCCGAACCATAAGGCGCGTGACGGAAAATAAAGGCAAGTTTACTCATAGGGTTATCACCCGATCGGCTTGTAATAAGGCTTGGCTGAA‑TTCACCCAAGCCGGCAAGTTGAAAAACGTCCGCCAGGTTAACCGCACTTTTATCGGCGGCAGAATAGCTATCCACCACGCCACGCCGTTGAGCGGCGGCAACGCACAAGTGCAGCgg                                                                                                                                                                                                                                             >  1:316948/1‑228 (MQ=255)

                           cgTCTAATCCTTCGCGTGTATTTGCCGAACCATAAGGCGCGTGACGGAAAATAAAGGCAAGTTTACTCATAGGGTTATCACCCGATCGGCTTGTAATAAGGCTTGGCTGAA‑TTCACCCAAGCCGGCAAGTTGAAAAACGTCCGCCAGGTTAACCGCACTTTTATCGGCGGCAGAATAGCTATCCACCACGCCACGCCGTTGAGCGGCGGCAACGCACAAGTGCAGCGGCACGTTAAATTGACGGGAAAAtg                                                                                                                                                                                                                      >  1:477069/1‑251 (MQ=255)

                             tCTAATCCTTCGCGTGTATTTGCCGAACCATAAGGCGCGTGACGGAAAATAAAGGCAAGTTTACTCATAGGGTTATCACCCGATCGGCTTGTAATAAGGCTTGGCTGAA‑TTCACCCAAGCCGGCAAGTTGAAAAACGTCCGCCAGGTTAACCGCACTTTTATCGGCGGCAGAATAGCTATCCACCACGCCACGCCGTTGAGCGGCGGCAACGCACAAGTGCAGCGGCACGTTAAATTGACGGGAAAAtgc                                                                                                                                                                                                                     >  1:170660/1‑250 (MQ=255)

                                            gtATTTGCCGAACCATAAGGCGCGTGACGGAAAATAAAGGCAAGTTTACTCATAGGGTTATCACCCGATCGGCTTGTAATAAGGCTTGGCTGAA‑TTCACCCAAGCCGGCAAGTTGAAAAACGTCCGCCAGGTTAACCGCACTTTTATCGGCGGCAGAATAGCTATCCACCACGCCACGCCGTTGAGCGGCGGCAACGCACAAGTGCAGCGGCACGTTAAATTGACGGGAAAATGCCTGCCAGGCTTGGAc                                                                                                                                                                                                      >  1:567448/1‑250 (MQ=255)

                                             tATTTGCCGAACCATAAGGCGCGTGACGGAAAATAAAGGCAAGTTTACTCATAGGGTTATCACCCGATCGGCTTGTAATAAGGCTTGGCTGAA‑TTCACCCAAGCCGGCAAGTTGAAAAACGTCCGCCAGGTTAACCGCACTTTTATCGGCGGCAGAATAGCTATCCACCACGCCACGCCGTTGAGCGGCGGCAACGCACAAGTGCAGCGGCACGTTAAATTGACGGGAAAATGCCTGCCAGGCTTGGAcc                                                                                                                                                                                                     <  1:270052/250‑1 (MQ=255)

                                                     gAACCATAAGGCGCGTGACGGAAAATAAAGGCAAGTTTACTCATAGGGTTATCACCCGATCGGCTTGTAATAAGGCTTGGCTGAA‑TTCACCCAAGCCGGCAAGTTGAAAAACGTCCGCCAGGTTAACCGCACTTTTATCGGCGGCAGAATAGCTATCCACCACGCCACGCCGTTGAGCGGCGGCAACGCACAAGTGCAGCGGCACGTTAAATTGACGGGAAAATGCCTGCCAGGCTTGGACCaaattaaat                                                                                                                                                                                            >  1:178229/1‑251 (MQ=255)

                                                      aaCCATAAGGCGCGTGACGGAAAATAAAGGCAAGTTTACTCATAGGGTTATCACCCGATCGGCTTGTAATAAGGCTTGGCTGAA‑TTCACCCAAGCCGGCAAGTTGAAAAACGTCCGCCAGGTTAACCGCACTTTTATCGGCGGCAGAATAGCTATCCACCACGCCACGCCGTTGAGCGGCGGCAACGCACAAGTGCAGCGGCACGTTAAATTGACGGGAAAATGCCTGCCAGGCTTGGACCaaattaaatt                                                                                                                                                                                           <  1:322597/251‑1 (MQ=255)

                                                      aaCCATAAGGCGCGTGACGGAAAATAAAGGCAAGTTTACTCATAGGGTTATCACCCGATCGGCTTGTAATAAGGCTTGGCTGAA‑TTCACCCAAGCCGGCAAGTTGAAAAACGTCCGCCAGGTTAACCGCACTTTTATCGGCGGCAGAATAGCTATCCACCACGCCACGCCGTTGAGCGGCGGCAACGCACAAGTGCAGCGGCACGTTAAATTGACGGGAAAATGCCTGCCAGGCTTGGACCaaattaaat                                                                                                                                                                                            <  1:640949/250‑1 (MQ=255)

                                                       aCCATAAGGCGCGTGACGGAAAATAAAGGCAAGTTTACTCATAGGGTTATCACCCGATCGGCTTGTAATAAGGCTTGGCTGAA‑TTCACCCAAGCCGGCAAGTTGAAAAACGTCCGCCAGGTTAACCGCACTTTTATCGGCGGCAGAATAGCTATCCACCACGCCACGCCGTTGAGCGGCGGCAACGCACAAGTGCAGCGGCACGTTAAATTGACGGGAAAATGCCTGCCAGGCt                                                                                                                                                                                                           <  1:174415/234‑1 (MQ=255)

                                                       aCCATAAGGCGCGTGACGGAAAATAAAGGCAAGTTTACTCATAGGGTTATCACCCGATCGGCTTGTAATAAGGCTTGGCTGAA‑TTCACCCAAGCCGGCAAGTTGAAAAACGTCCGCCAGGTTAACCGCACTTTTATCGGCGGCAGAATAGCTATCCACCACGCCACGCCGTTGAGCGGCGGCAACGCACAAGTGCAGCGGCACGTTAAATTGACGGGAAAATGCCTGCCAGGCTTGGACCaaattaaatt                                                                                                                                                                                           >  1:677888/1‑250 (MQ=255)

                                                                cgcgTGACGGAAAATAAAGGCAAGTTTACTCATAGGGTTATCACCCGATCGGCTTGTAATAAGGCTTGGCTGAA‑TTCACCCAAGCCGGCAAGTTGAAAAACGTCCGCCAGGTTAACCGCACTTTTATCGGCGGCAGAATAGCTATCCACCACGCCACGCCGTTGAGCGGCGGCAACGCACAAGTGCAGCGGCACGTTAAATTGACGGGAAAATGCCTGCCAGGCTTGGACCAAATTAAATTCGTCATTAg                                                                                                                                                                                  <  1:388157/250‑1 (MQ=255)

                                                                  cgTGACGGAAAATAAAGGCAAGTTTACTCATAGGGTTATCACCCGATCGGCTTGTAATAAGGCTTGGCTGAA‑TTCACCCAAGCCGGCAAGTTGAAAAACGTCCGCCAGGTTAACCGCACTTTTATCGGCGGCAGAATAGCTATCCACCACGCCACGCCGTTGAGCGGCGGCAACGCACAAGTGCAGCGGCACGTTAAATTGACGGGAAAATGCCTGCCAGGCTTGGACCAAATTAAATTCGTCATTAGCg                                                                                                                                                                                <  1:614593/250‑1 (MQ=255)

                                                                          aaaaTAAAGGCAAGTTTACTCATAGGGTTATCACCCGATCGGCTTGTAATAAGGCTTGGCTGAA‑TTCACCCAAGCCGGCAAGTTGAAAAACGTCCGCCAGGTTAACCGCACTTTTATCGGCGGCAGAATAGCTATCCACCACGCCACGCCGTTGAGCGGCGGCAACGCACAAGTGCAGCGGCACGTTAAATTGACGGGAAAATGCCTGCCAGGCTTGGACCAAATTAAATTCGTCATTAGCGGGATAAAcc                                                                                                                                                                       <  1:188902/251‑1 (MQ=255)

                                                                               aaaGGCAAGTTTACTCATAGGGTTATCACCCGATCGGCTTGTAATAAGGCTTGGCTGAA‑TTCACCCAAGCCGGCAAGTTGAAAAACGTCCGCCAGGTTAACCGCACTTTTATCGGCGGCAGAATAGCTATCCACCACGCCACGCCGTTGAGCGGCGGCAACGCACAAGTGCAGCGGCACGTTAAATTGACGGGAAAATGCCTGCCAGGCTTGGACCAAATTAAATTCGTCATTAGCGGGATAAACCAAGGc                                                                                                                                                                  <  1:144196/251‑1 (MQ=255)

                                                                               aaaGGCAAGTTTACTCATAGGGTTATCACCCGATCGGCTTGTAATAAGGCTTGGCTGAA‑TTCACCCAAGCCGGCAAGTTGAAAAACGTCCGCCAGGTTAACCGCACTTTTATCGGCGGCAGAATAGCTATCCACCACGCCACGCCGTTGAGCGGCGGCAACGCACAAGTGCAGCGGCACGTTAAATTGACGGGAAAATGCCTGCCAGGCTTGGACCAAATTAAATTCGTCATTAGCGGGATAAACCAAGGc                                                                                                                                                                  <  1:825401/251‑1 (MQ=255)

                                                                                      aGTTTACTCATAGGGTTATCACCCGATCGGCTTGTAATAAGGCTTGGCTGAA‑TTCACCCAAGCCGGCAAGTTGAAAAACGTCCGCCAGGTTAACCGCACTTTTATCGGCGGCAGAATAGCTATCCACCACGCCACGCCGTTGAGCGGCGGCAACGCACAAGTGCAGCGGCACGTTAAATTGACGGGAAAATGCCTGCCAGGCTTGGACCAAATTAAATTCGTCATTAGCGGGATAAACCAAGGCGTTGCCg                                                                                                                                                           >  1:621243/1‑251 (MQ=255)

                                                                                      aGTTTACTCATAGGGTTATCACCCGATCGGCTTGTAATAAGGCTTGGCTGAA‑TTCACCCAAGCCGGCAAGTTGAAAAACGTCCGCCAGGTTAACCGCACTTTTATCGGCGGCAGAATAGCTATCCACCACGCCACGCCGTTGAGCGGCGGCAACGCACAAGTGCAGCGGCACGTTAAATTGACGGGAAAATGCCTGCCAGGCTTGGACCAAATTAAATTCGTCATTAGCGGGATAAACCAAGGCGTTGCCg                                                                                                                                                           >  1:104243/1‑251 (MQ=255)

                                                                                      aGTTTACTCATAGGGTTATCACCCGATCGGCTTGTAATAAGGCTTGGCTGAA‑TTCACCCAAGCCGGCAAGTTGAAAAACGTCCGCCAGGTTAACCGCACTTTTATCGGCGGCAGAATAGCTATCCACCACGCCACGCCGTTGAGCGGCGGCAACGCACAAGTGCAGCGGCACGTTAAATTGACGGGAAAATGCCTGCCAGGCTTGGACCAAATTAAATTCGTCATTAGCGGGATAAACCAAGGCGTTGCCg                                                                                                                                                           >  1:706868/1‑251 (MQ=255)

                                                                                           aCTCATAGGGTTATCACCCGATCGGCTTGTAATAAGGCTTGGCTGAA‑TTCACCCAAGCCGGCAAGTTGAAAAACGTCCGCCAGGTTAACCGCACTTTTATCGGCGGCGGAATAGCTATCCACCACGCCACGCCGTTGAGCGGCGGCAACGCACAAGTGCAGCGGCACGTTAAATTGACGGGAAAATGCCTGCCAGGCTTGGACCAAATTAAATTCGTCATTAGCGGGATAAACCAAGGCGTTGCCGTTGGt                                                                                                                                                      <  1:307075/251‑1 (MQ=255)

                                                                                            cTCATAGGGTTATCACCCGATCGGCTTGTAATAAGGCTTGGCTGAATTTCACCCAAGCCGGCAAGTTGAAAAACGTCCGCCAGGTTAACCGCACTTTTATCGGCGGCAGAATAGCTATCCACCACGCCACGCCGTTGAGCGGCGGCAACGCACAAGTGCAGCGGCACGTTAAATTGACGGGAAAATGCCTGCCAGGCt                                                                                                                                                                                                           <  1:824438/198‑1 (MQ=255)

                                                                                            cTCATAGGGTTATCACCCGATCGGCTTGTAATAAGGCTTGGCTGAA‑TTCACCCAAGCCGGCAAGTTGAAAAACGTCCGCCAGGTTAACCGCACTTTTATCGGCGGCAGAATAGCTATCCACCACGCCACGCCGTTGAGCGGCGGCAACGCACAAGTGc                                                                                                                                                                                                                                                  >  1:215485/1‑158 (MQ=255)

                                                                                            cTCATAGGGTTATCACCCGATCGGCTTGTAATAAGGCTTGGCTGAA‑TTCACCCAAGCCGGCAAGTTGAAAAACGTCCGCCAGGTTAACCGCACTTTTATCGGCGGCAGAATAGCTATCCACCACGCCACGCCGTTGAGCGGCGGCAACGCACAAGTGCAGCGGCACGTTAAAt                                                                                                                                                                                                                                   >  1:569538/1‑173 (MQ=255)

                                                                                            cTCATAGGGTTATCACCCGATCGGCTTGTAATAAGGCTTGGCTGAA‑TTCACCCAAGCCGGCAAGTTGAAAAACGTCCGCCAGGTTAACCGCACTTTTATCGGCGGCAGAATAGCTATCCACCACGCCACGCCGTTGAGCGGCGGCAACGCACAAGTGCAGCGGCACGTTAAATTGACGGGAAAATGCCTGCCAGGCTTGGACCAAATTAAATTCGTCATTAGCGGGATAAACCAAGGCGTTGCCGTTGGtt                                                                                                                                                     >  1:394842/1‑251 (MQ=255)

                                                                                            cTCATAGGGTTATCACCCGATCGGCTTGTAATAAGGCTTGGCTGAA‑TTCACCCAAGCCGGCAAGTTGAAAAACGTCCGCCAGGTTAACCGCACTTTTATCGGCGGCAGAATAGCTATCCACCACGCCACGCCGTTGAGCGGCGGCAACGCACAAGTGCAGCGGCACGTTAAATTGACGGGAAAATGCCTGCCAGGCTTGGACCAAATTAAATTCGTCATTAGCGGGATAAACCAAGGCGTTGCCGTTGGtt                                                                                                                                                     >  1:202929/1‑251 (MQ=255)

                                                                                            cTCATAGGGTTATCACCCGATCGGCTTGTAATAAGGCTTGGCTGAA‑TTCACCCAAGCCGGCAAGTTGAAAAACGTCCGCCAGGTTAACCGCACTTTTATCGGCGGCAGAATAGCTATCCACCACGCCACGCCGTTGAGCGGCGGCAACGCACAAGTGCAGCGGCACGTTAAATTGACGGGAAAATGCCTGCCAGGCTTGGACCAAATTAAATTCGTCATTAGCGGGATAAACCAAGGCGTTGCCGTTGGtt                                                                                                                                                     <  1:860549/251‑1 (MQ=255)

                                                                                            cTCATAGGGTTATCACCCGATCGGCTTGTAATAAGGCTTGGCTGAA‑TTCACCCAAGCCGGCAAGTTGAAAAACGTCCGCCAGGTTAACCGCACTTTTATCGGCGGCAGAATAGCTATCCACCACGCCACGCCGTTGAGCGGCGGCAACGCACAAGTGCAGCGGCACGTTAAATTGACGGGAAAATGCCTGCCAGGCTTGGACCAAATTAAATTCGTCATTAGCGGGATAAACCAAGGCGTTGCCGTTGGtt                                                                                                                                                     >  1:814494/1‑251 (MQ=255)

                                                                                                  gggTTATCACCCGATCGGCTTGTAATAAGGCTTGGCTGAA‑TTCACCCAAGCCGGCAAGTTGAAAAACGTCCGCCAGGTTAACCGCACTTTTATCGGCGGCAGAATAGCTATCCACCACGCCACGCCGTTGAGCGGCGGCAACGCACAAGTGCAGCGGCACGTTAAATTGACGGGAAAATGCCTGCCAGGCTTGGACCAAATTAAATTCGTCATTAGCGGGATAAACCAAGGCGTTGCCGTTGGTTACGCCa                                                                                                                                               >  1:421986/1‑251 (MQ=255)

                                                                                                   ggTTATCACCCGATCGGCTTGTAATAAGGCTTGGCTGAA‑TTCACCCAAGCCGGCAAGTTGAAAAACGTCCGCCAGGTTAACCGCACTTTTATCGGCGGCAGAATAGCTATCCACCACGCCACGCCGTTGAGCGGCGGCAACGCACAAGTGCAGCGGCACGTTAAATTGACGGGAAAATGCCTGCCAGGCTTGGACCAAATTAAATTCGTCATTAGCGGGATAAACCAAGGCGTTGCCGTTGGTTACGcc                                                                                                                                                <  1:816442/249‑1 (MQ=255)

                                                                                                    gTTATCACCCGATCGGCTTGTAATAAGGCTTGGCTGAA‑TTCACCCAAGCCGGCAAGTTGAAAAACGTCCGCCAGGTTAACCGCACTTTTATCGGCGGCAGAATAGCTATCCACCACGCCACGCCGTTGAGCGGCGGCAACGCACAAGTGCAGCGGCACGTTAAATTGACGGGAAAATGCCTGCCAGGCt                                                                                                                                                                                                           >  1:289480/1‑189 (MQ=255)

                                                                                                    gTTATCACCCGATCGGCTTGTAATAAGGCTTGGCTGAA‑TTCACCCAAGCCGGCAAGTTGAAAAACGTCCGCCAGGTTAACCGCACTTTTATCGGCGGCAGAATAGCTATCCACCACGCCACGCCGTTGAGCGGCGGCAACGCACAAGTGCAGCGGCACGTTAAATTGACGGGAAAATGCCTGCCAGGCTTGGACCAAATTAAATTCGTCATTAGCGGGATAAACCAAGGCGTTGCCGTTGGTTACGCCAt                                                                                                                                              >  1:845770/1‑250 (MQ=255)

                                                                                                    gTTATCACCCGATCGGCTTGTAATAAGGCTTGGCTGAA‑TTCACCCAAGCCGGCAAGTTGAAAAACGTCCGCCAGGTTAACCGCACTTTTATCGGCGGCAGAATAGCTATCCACCACGCCACGCCGTTGAGCGGCGGCAACGCACAAGTGCAGCGGCACGTTAAATTGACGGGAAAATGCCTGCCAGGCTTGGACCAAATTAAATTCGTCATTAGCGGGATAAACCAAGGCGTTGCCGTTGGTTACGCCAt                                                                                                                                              >  1:651134/1‑250 (MQ=255)

                                                                                                    gTTATCACCCGATCGGCTTGTAATAAGGCTTGGCTGAA‑TTCACCCAAGCCGGCAAGTTGAAAAACGTCCGCCAGGTTAACCGCACTTTTATCGGCGGCAGAATAGCTATCCACCACGCCACGCCGTTGAGCGGCGGCAACGCACAAGTGCAGCGGCACGTTAAATTGACGGGAAAATGCCTGCCAGGCTTGGACCAAATTAAATTCGTCATTAGCGGGATAAACCAAGGCGTTGCCGTTGGTTACGCCAt                                                                                                                                              >  1:260862/1‑250 (MQ=255)

                                                                                                    gTTATCACCCGATCGGCTTGTAATAAGGCTTGGCTGAA‑TTCACCCAAGCCGGCAAGTTGAAAAACGTCCGCCAGGTTAACCGCACTTTTATCGGCGGCAGAATAGCTATCCACCACGCCACGCCGTTGAGCGGCGGCAACGCACAAGTGCAGCGGCACGTTAAATTGACGGGAAAATGCCTGCCAGGCTTGGACCAAATTAAATTCGTCATTAGCGGGATAAACCAAGGCGTTGCCGTTGGTTACGCCAt                                                                                                                                              >  1:352302/1‑250 (MQ=255)

                                                                                                     ttATCACCCGATCGGCTTGTAATAAGGCTTGGCTGAA‑TTCACCCAAGCCGGCAAGTTGAAAAACGTCCGCCAGGTTAACCGCACTTTTATCGGCGGCAGAATAGCTATCCACCACGCCACGCCGTTGAGCGGCGGCAACGCACAAGTGCAGCGGCACGTTAAATTGACGGGAAAATGCCTGCCAGGCTTGGACCAAATTAAATTCGTCATTAGCGGGATAAACCAAGGCGTTGCCGTTGGTTACGCCATcc                                                                                                                                            <  1:839984/251‑1 (MQ=255)

                                                                                                     ttATCACCCGATCGGCTTGTAATAAGGCTTGGCTGAA‑TTCACCCAAGCCGGCAAGTTGAAAAACGTCCGCCAGGTTAACCGCACTTTTATCGGCGGCAGAATAGCTATCCACCACGCCACGCCGTTGAGCGGCGGCAACGCACAAGTGCAGCGGCACGTTAAATTGACGGGAAAATGCCTGCCAGGCTTGGACCAAATTAAATTCGTCATTAGCGGGATAAACCAAGGCGTTGCCGTTGGTTACGCCATcc                                                                                                                                            <  1:690642/251‑1 (MQ=255)

                                                                                                     ttATCACCCGATCGGCTTGTAATAAGGCTTGGCTGAA‑TTCACCCAAGCCGGCAAGTTGAAAAACGTCCGCCAGGTTAACCGCACTTTTATCGGCGGCAGAATAGCTATCCACCACGCCACGCCGTTGAGCGGCGGCAACGCACAAGTGCAGCGGCACGTTAAATTGACGGGAAAATGCCTGCCAGGCTTGGACCAAATTAAATTCGTCATTAGCGGGATAAACCAAGGCGTTGCCGTTGGTTACGCCATcc                                                                                                                                            <  1:764509/251‑1 (MQ=255)

                                                                                                                   gCTTGTAATAAGGCTTGGCTGAA‑TTCACCCAAGCCGGCAAGTTGAAAAACGTCCGCCAGGTTAACCGCACTTTTATCGGCGGCAGAATAGCTATCCACCACGCCACGCCGTTGAGCGGCGGCAACGCACAAGTGCAGCGGCACGTTAAATTGACGGGAAAATGCCTGCCAGGCTTGGACCAAATTAAATTCGTCATTAg                                                                                                                                                                                  <  1:873399/199‑1 (MQ=255)

                                                                                                                   gCTTGTAATAAGGCTTGGCTGAA‑TTCACCCAAGCCGGCAAGTTGAAAAACGTCCGCCAGGTTAACCGCACTTTTATCGGCGGCAGAATAGCTATCCACCACGCCACGCCGTTGAGCGGCGGCAACGCACAAGTGCAGCGGCACGTTAAATTGACGGGAAAATGCCTGCCAGGCTTGGACCAAATTAAATTCGTCATTAGCGGGATAAACCAAGGCGTTGCCGTTGGTTACGCCATCCTGATAAAAAAAGAt                                                                                                                              >  1:404154/1‑251 (MQ=255)

                                                                                                                       gTAATAAGGCTTGGCTGAA‑TTCACCCAAGCCGGCAAGTTGAAAAACGTCCGCCAGGTTAACCGCACTTTTATCGGCGGCAGAATAGCTATCCACCACGCCACGCCGTTGAGCGGCGGCAACGCACAAGTGc                                                                                                                                                                                                                                                  >  1:828763/1‑131 (MQ=255)

                                                                                                                       gTAATAAGGCTTGGCTGAA‑TTCACCCAAGCCGGCAAGTTGAAAAACGTCCGCCAGGTTAACCGCACTTTTATCGGCGGCAGAATAGCTATCCACCACGCCACGCCGTTGAGCGGCGGCAACGCACAAGTGCAGCGGCACGTTAAATTGACGGGAAAATGCCTGCCAGGCTTGGACCAAATTAAATTCGTCATTAGCGGGATAAACCAAgg                                                                                                                                                                   <  1:392645/210‑1 (MQ=255)

                                                                                                                       gTAATAAGGCTTGGCTGAA‑TTCACCCAAGCCGGCAAGTTGAAAAACGTCCGCCAGGTTAACCGCACTTTTATCGGCGGCAGAATAGCTATCCACCACGCCACGCCGTTGAGCGGCGGCAACGCACAAGTGCAGCGGCACGTTAAATTGACGGGAAAATGCCTGCCAGGCTTGGACCAAATTAAATTCGTCATTAGCGGGATAAACCAAGGCGTTGCCGTTGGTTACGcc                                                                                                                                                <  1:307961/229‑1 (MQ=255)

                                                                                                                       gTAATAAGGCTTGGCTGAA‑TTCACCCAAGCCGGCAAGTTGAAAAACGTCCGCCAGGTTAACCGCACTTTTATCGGCGGCAGAATAGCTATCCACCACGCCACGCCGTTGAGCGGCGGCAACGCACAAGTGCAGCGGCACGTTAAATTGACGGGAAAATGCCTGCCAGGCTTGGACCAAATTAAATTCGTCATTAGCGGGATAAACCAAGGCGTTGCCGTTGGTTACGCCATCCTGATAAAAAAAGATTTgg                                                                                                                          >  1:667117/1‑251 (MQ=255)

                                                                                                                       gTAATAAGGCTTGGCTGAA‑TTCACCCAAGCCGGCAAGTTGAAAAACGTCCGCCAGGTTAACCGCACTTTTATCGGCGGCAGAATAGCTATCCACCACGCCACGCCGTTGAGCGGCGGCAACGCACAAGTGCAGCGGCACGTTAAATTGACGGGAAAATGCCTGCCAGGCTTGGACCAAATTAAATTCGTCATTAGCGGGATAAACCAAGGCGTTGCCGTTGGTTACGCCATCCTGATAAAAAAAGATTTgg                                                                                                                          >  1:19014/1‑251 (MQ=255)

                                                                                                                        taataaGGCTTGGCTGAA‑TTCACCCAAGCCGGCAAGTTGAAAAACGTCCGCCAGGTTAACCGCACTTTTATCGGCGGCAGAATAGCTATCCACCACGCCACGCCGTTGAGCGGCGGCAACGCACAAGTGCAGCGGCACGTTAAATTGACGGGAAAATGCCTGCCAGGCTTGGACCAAATTAAATTCGTCATTAGCGGGATAAACCAAGGCGTTGCCGTTGGTTACGCCATCCTGATAAAAAAAGATTTGGc                                                                                                                         <  1:895335/251‑1 (MQ=255)

                                                                                                                              ggCTTGGCTGAA‑TTCACCCAAGCCGGCAAGTTGAAAAACGTCCGCCAGGTTAACCGCACTTTTATCGGCGGCAGAATAGCTATCCACCACGCCACGCCGTTGAGCGGCGGCAACGCACAAGTGCAGCGGCACGTTAAATTGACGGGAAAATGCCTGCCAGGCTTGGACCAAATTAAATTCGTCATTAGCGGGATAAACCAAGGCGTTGCCGTTGGTTACGCCATCCTGATAAAAAAAGATTTGGCTGatat                                                                                                                   >  1:264276/1‑251 (MQ=255)

                                                                                                                                    gCTGAA‑TTCACCCAAGCCGGCAAGTTGAAAAACGTCCGCCAGGTTAACCGCACTTTTATCGGCGGCAGAATAGCTATCCATCACGCCACGCCGTTGAGCGGCGGCAACGCACAAGTGCAGCGGCACGTTAAATTGACGGGAAAATGCCTGCCAGGCTTGGACCAAATTAAATTCGTCATTAGCGGGATAAACCAAGGCGTTGCCGTTGGTTACGCCATCCTGATAAAAAAAGATTTGGCTGATATGATGc                                                                                                              <  1:435874/250‑1 (MQ=255)

                                                                                                                                    gCTGAA‑TTCACCCAAGCCGGCAAGTTGAAAAACGTCCGCCAGGTTAACCGCACTTTTATCGGCGGCAGAATAGCTATCCACCACGCCACGCCGTTGAGCGGCGGCAACGCACAAGTGCAGCGGCACGTTAAATTGACGGGAAAATGCCTGCCAGGCTTGGACCAAATTAAATTCGTCATTAGCGGGATAAACCAAGGCGTTGCCGTTGGTTACGCCATCCTGATAAAAAAAGATTTGGCTGATATGATGc                                                                                                              <  1:899276/250‑1 (MQ=255)

                                                                                                                                       gAA‑TTCACCCAAGCCGGCAAGTTGAAAAACGTCCGCCAGGTTAACCGCACTTTTATCGGCGGCAGAATAGCTATCCACCACGCCACGCCGTTGAGCGGCGGCAACGCACAAGTGCAGCGGCACGTTAAATTGACGGGAAAATGCCTGCCAGGCTTGGACCAAATTAAATTCGTCATTAGCGGGATAAACCAAgg                                                                                                                                                                   <  1:136313/194‑1 (MQ=255)

                                                                                                                                        aa‑TTCACCCAAGCCGGCAAGTTGAAAAACGTCCGCCAGGTTAACCGCACTTTTATCGGCGGCAGAATAGCTATCCACCACGCCACGCCGTTGAGCGGCGGCAACGCACAAGTGCAGCGGCACGTTAAATTGACGGGAAAATGCCTGCCAGGCTTGGACCAAATTAAATTCGTCATTAGCGGGATAAAcc                                                                                                                                                                       >  1:639021/1‑189 (MQ=255)

                                                                                                                                            tCACCCAAGCCGGCAAGTTGAAAAACGTCCGCCAGGTTAACCGCACTTTTATCGGCGGCAGAATAGCTATCCACCACGCCACGCCGTTGAGCGGCGGCAACGCACAAGTGCAGCGGCACGTTAAATTGACGGGAAAATGCCTGCCAGGCTTGGACCAAATTAAATTCGTCATTAGCGGGATAAACCAAGGCGTTGCCGTTGGTTACGCCATCCTGATAAAAAAAGATTTGGCTGATATGATGCTTTTGCAc                                                                                                      <  1:845686/251‑1 (MQ=255)

                                                                                                                                            tCACCCAAGCCGGCAAGTTGAAAAACGTCCGCCAGGTTAACCGCACTTTTATCGGCGGCAGAATAGCTATCCACCACGCCACGCCGTTGAGCGGCGGCAACGCACAAGTGCAGCGGCACGTTAAATTGACGGGAAAATGCCTGCCAGGCTTGGACCAAATTAAATTCGTCATTAGCGGGATAAACCAAGGCGTTGCCGTTGGTTACGCCATCCTGATAAAAAAAGATTTGGCTGATATGATGCTTTTGCAc                                                                                                      <  1:325617/251‑1 (MQ=255)

                                                                                                                                             cACCCAAGCCGGCAAGTTGAAAAACGTCCGCCAGGTTAACCGCACTTTTATCGGCGGCAGAATAGCTATCCACCACGCCACGCCGTTGAGCGGCGGCAACGCACAAGTGCAGCGGCACGTTAAATTGACGGGAAAATGCCTGCCAGGCTTGGACCAAATTAAATTCGTCATTAGCGGGATAAACCAAGGCGTTGCCGTTGGTTACGCCATCCTGATAAAAAAAGATTTGGCTGATATGCTGCTTTTGCAc                                                                                                      >  1:277490/1‑250 (MQ=255)

                                                                                                                                             cACCCAAGCCGGCAAGTTGAAAAACGTCCGCCAGGTTAACCGCACTTTTATCGGCGGCAGAATAGCTATCCACCACGCCACGCCGTTGAGCGGCGGCAACGCACAAGTGCAGCGGCACGTTAAATTGACGGGAAAATGCCTGCCAGGCTTGGACCAAATTAAATTCGTCATTAGCGGGATAAACCAAGGCGTTGCCGTTGGTTACGCCATCCTGATAAAAAAAGATTTGGCTGATATGATGCTTTTGCAcc                                                                                                     <  1:586561/251‑1 (MQ=255)

                                                                                                                                             cACCCAAGCCGGCAAGTTGAAAAACGTCCGCCAGGTTAACCGCACTTTTATCGGCGGCAGAATAGCTATCCACCACGCCACGCCGTTGAGCGGCGGCAACGCACAAGTGCAGCGGCACGTTAAATTGACGGGAAAATGCCTGCCAGGCTTGGACCAAATTAAATTCGTCATTAGCGGGATAAACCAAGGCGTTGCCGTTGGTTACGCCATCCTGATAAAAAAAGATTTGGCTGATATGATGCTTTTGCAc                                                                                                      >  1:504783/1‑250 (MQ=255)

                                                                                                                                                  aaGCCGGCAAGTTGAAAAACGTCCGCCAGGTTAACCGCACTTTTATCGGCGGCAGAATAGCTATCCACCACGCCACGCCGTTGAGCGGCGGCAACGCACAAGTGCAGCGGCACGTTAAATTGACGGGAAAATGCCTGCCAGGCTTGGACCAAATTAAATTCGTCATTAGCGGGATAAACCAAGGCGTTGCCGTTGGTTACGCCATCCTGATAAAAAAAGATTTGGCTGATATGATGCTTTTGCACCAGTAc                                                                                                <  1:139404/251‑1 (MQ=255)

                                                                                                                                                  aaGCCGGCAAGTTGAAAAACGTCCGCCAGGTTAACCGCACTTTTATCGGCGGCAGAATAGCTATCCACCACGCCACGCCGTTGAGCGGCGGCAACGCACAAGTGCAGCGGCACGTTAAATTGACGGGAAAATGCCTGCCAGGCTTGGACCAAATTAAATTCGTCATTAGCGGGATAAACCAAGGCGTTGCCGTTGGTTACGCCATCCTGATAAAAAAAGATTTGGCTGATATGATGCTTTTGCACCAGTAc                                                                                                <  1:203484/251‑1 (MQ=255)

                                                                                                                                                        gCAAGTTGAAAAACGTCCGCCAGGTTAACCGCACTTTTATCGGCGGCAGAATAGCTATCCACCACGCCACGCCGTTGAGCGGCGGCAACGCACAAGTGCAGCGGCACGTTAAATTGACGGGAAAATGCCTGCCAGGCTTGGACCAAATTAAATTCGTCATTAGCGGGATAAACCAAGGCGTTGCCGTTGGTTACGCCATCCTGATAAAAAAAGATTTGGCTGATATGATGCTTTTGCACCAGTACTtgtg                                                                                           <  1:348197/250‑1 (MQ=255)

                                                                                                                                                            gTTGAAAAACGTCCGCCAGGTTAACCGCACTTTTATCGGCGGCAGAATAGCTATCCACCACGCCACGCCGTTGAGCGGCGGCAACGCACAAGTGCAGCGGCACGTTAAATTGACGGGAAAATGCCTGCCAGGCTTGGAcc                                                                                                                                                                                                     >  1:148977/1‑140 (MQ=255)

                                                                                                                                                            gTTGAAAAACGTCCGCCAGGTTAACCGCACTTTTATCGGCGGCAGAATAGCTATCCACCACGCCACGCCGTTGAGCGGCGGCAACGCACAAGTGCAGCGGCACGTTAAATTGACGGGAAAATGCCTGCCAGGCTTGGACCaaattaaat                                                                                                                                                                                            >  1:240885/1‑149 (MQ=255)

                                                                                                                                                            gTTGAAAAACGTCCGCCAGGTTAACCGCACTTTTATCGGCGGCAGAATAGCTATCCACCACGCCACGCCGTTGAGCGGCGGCAACGCACAAGTGCAGCGGCACGTTAAATTGACGGGAAAATGCCTGCCAGGCTTGGACCAAATTAAATTCGTCATTAGCGGGATAAACCAAGGCGTTGCCGTTGGTTACGCCATCCTGATa                                                                                                                                       <  1:341652/202‑1 (MQ=255)

                                                                                                                                                             ttGAAAAACGTCCGCCAGGTTAACCGCACTTTTATCGGCGGCAGAATAGCTATCCACCACGCCACGCCGTTGAGCGGCGGCAACGCACAAGTGCAGCGGCACGTTAAATTGACGGGAAAATGCCTGCCAGGCTTGGACCAAATTAAATTCGTCATTAGCGGGATAAACCAAGGCGTTGCCGTTGGTTACGCCATCCTGATAAAAAAAGATTTGGCTGATATGATGCTTTTGCACCAGTACTTGTGCCAGtt                                                                                     <  1:726649/251‑1 (MQ=255)

                                                                                                                                                             ttGAAAAACGTCCGCCAGGTTAACCGCACTTTTATCGGCGGCAGAATAGCTATCCACCACGCCACGCCGTTGAGCGGCGGCAACGCACAAGTGCAGCGGCACGTTAAATTGACGGGAAAATGCCTGCCAGGCTTGGACCAAATTAAATTCGTCATTAGCGGGATAAACCAAGGCGTTGCCGTTGGTTACGCCATCCTGATAAAAAAAGATTTGGCTGATATGATGCTTTTGCACCAGTACTTGTGCCAGtt                                                                                     <  1:281054/251‑1 (MQ=255)

                                                                                                                                                                      gTCCGCCAGGTTAACCGCACTTTTATCGGCGGCAGAATAGCTATCCACCACGCCACGCCGTTGAGCGGCGGCAACGCACAAGTGCAGCGGCACGTTAAATTGACGGGAAAATGCCTGCCAGGCTTGGACCAAATTAAATTCGTCATTAGCGGGATAAACCAAGGCGTTGCCGTTGGTTACGCCATCCTGATAAAAAAAGATTTGGCTGATATGATGCTTTTGCACCAGTAc                                                                                                <  1:306200/231‑1 (MQ=255)

                                                                                                                                                                               gTTAACCGCACTTTTATCGGCGGCAGAATAGCTATCCACCACGCCACGCCGTTGAGCGGCGGCAACGCACAAGTGCAGCGGCACGTTAAATTGACGGGAAAATGCCTGCCAGGCTTGGACCAAATTAAATTCGTCATTAGCGGGATAAACCAAGGCGTTGCCGTTGGTTACGCCATCCTGATAAAAAAAGATTTGGCTGATATGATGCTTTTGCACCAGTACTTGTGcc                                                                                         <  1:605101/229‑1 (MQ=255)

                                                                                                                                                                                       cACTTTTATCGGCGGCAGAATAGCTATCCACCACGCCACGCCGTTGAGCGGCGGCAACGCACAAGTGCAGCGGCACGTTAAATTGACGGGAAAATGCCTGCCAGGCTTGGACCAAATTAAATTCGTCATTAGCGGGATAAACCAAGGCGTTGCCGTTGGTTACGCCATCCTGATAAAAAAAGATTTGGCTGATATGATGCTTTTGCACCAGTACTTGTGCCAGTTGGTACGCCAGAAACGCGCCTTGACt                                                            <  1:166645/250‑1 (MQ=255)

                                                                                                                                                                                       cACTTTTATCGGCGGCAGAATAGCTATCCACCACGCCACGCCGTTGAGCGGCGGCAACGCACAAGTGCAGCGGCACGTTAAATTGACGGGAAAATGCCTGCCAGGCTTGGACCAAATTAAATTCGTCATTAGCGGGATAAACCAAGGCGTTGCCGTTGGTTACGCCATCCTGATAAAAAAAGATTTGGCTGATATGATGCTTTTGCACCAGTACTTGTGCCAGTTGGTACGCCAGAAACGCGCCTTGACt                                                            <  1:44805/250‑1 (MQ=255)

                                                                                                                                                                                                     gcAGAATAGCTATCCACCACGCCACGCCGTTGAGCGGCGGCAACGCACAAGTGCAGCGGCACGTTAAATTGACGGGAAAATGCCTGCCAGGCTTGGACCAAATTAAATTCGTCATTAGCGGGATAAACCAAGGCGTTGCCGTTGGTTACGcc                                                                                                                                                >  1:742383/1‑152 (MQ=255)

                                                                                                                                                                                                     gcAGAATAGCTATCCACCACGCCACGCCGTTGAGCGGCGGCAACGCACAAGTGCAGCGGCACGTTAAATTGACGGGAAAATGCCTGCCAGGCTTGGACCAAATTAAATTCGTCATTAGCGGGATAAACCAAGGCGTTGCCGTTGGTTACGCCATCCTGATAAAAAAAGATTTGGCTGATATGATGCTTTTGCACCAGTACTTGTGCCAGTTGGTACGCCAGAAACGCGCCTTGACTACCGTAAACCGgttg                                             >  1:671460/1‑251 (MQ=255)

                                                                                                                                                                                                     gcAGAATAGCTATCCACCACGCCACGCCGTTGAGCGGCGGCAACGCACAAGTGCAGCGGCACGTTAAATTGACGGGAAAATGCCTGCCAGGCTTGGACCAAATTAAATTCGTCATTAGCGGGAT‑AACCAAGGCGTTGCCGTTGGTTACGCCATCCTGATAAAAAAAGATTTGGCTGATATGATGCTTTTGCACCAGTACTTGTGCCAGTTGGTACGCCAGAAACGCGCCTTGACTACCGTAAACCGgttgt                                            >  1:517399/1‑251 (MQ=255)

                                                                                                                                                                                                        gAATAGCTATCCACCACGCCACGCCGTTGAGCGGCGGCAACGCACAAGTGCAGCGGCACGTTAAATTGACGGGAAAATGCCTGCCAGGCTTGGACCAAATTAAATTCGTCATTAGCGGGATAAACCAAGGCGTTGCCGTTGGTTACGCCATCCTGATAAAAAAAGATTTGGCTGATATGATGCTTTTGCACCAGTACTTGTGCCAGTTGGTACGCCAGAAACGCGCCTTGACTACCGTAAACCGGTTGttt                                          >  1:169283/1‑251 (MQ=255)

                                                                                                                                                                                                        gAATAGCTATCCACCACGCCACGCCGTTGAGCGGCGGCAACGCACAAGTGCAGCGGCACGTTAAATTGACGGGAAAATGCCTGCCAGGCTTGGACCAAATTAAATTCGTCATTAGCGGGATAAACCAAGGCGTTGCCGTTGGTTACGCCATCCTGATAAAAAAAGATTTGGCTGATATGATGCTTTTGCACCAGTACTTGTGCCAGTTGGTACGCCAGAAACGCGCCTTGACTACCGTAAACCGGTTGttt                                          >  1:483302/1‑251 (MQ=255)

                                                                                                                                                                                                        gAATAGCTATCCACCACGCCACGCCGTTGAGCGGCGGCAACGCACAAGTGCAGCGGCACGTTAAATTGACGGGAAAATGCCTGCCAGGCTTGGACCAAATTAAATTCGTCATTAGCGGGATAAACCAAGGCGTTGCCGTTGGTTACGCCATCCTGATAAAAAAAGATTTGGCTGATATGATGCTTTTGCACCAGTACTTGTGCCAGTTGGTACGCCAGAAACGCGCCTTGACTACCGTAAACCGGTTGttt                                          >  1:134111/1‑251 (MQ=255)

                                                                                                                                                                                                           tAGCTATCCACCACGCCACGCCGTTGAGCGGCGGCAACGCACAAGTGCAGCGGCACGTTAAATTGACGGGAAAATGCCTGCCAGGCTTGGACCAAATTAAATTCGTCATTAGCGGGATAAACCAAGGCGTTGCCGTTGGTTACGCCATCCTGATAAAAAAAGATTTGGCTGATATGATGCTTTTGCACCAGTACTTGTGCCAGTTGGTACGCCAGAAACGCGCCTTGACTACCGTAAACCGGTTGTTTca                                        >  1:188209/1‑250 (MQ=255)

                                                                                                                                                                                                           tAGCTATCCACCACGCCACGCCGTTGAGCGGCGGCAACGCACAAGTGCAGCGGCACGTTAAACTGACGGGAAAATGCCTGCCAGGCTTGGACCAAATTAAATTCGTCATTAGCGGGATAAACCAAGGCGTTGCCGCTGGTTACGCCATCCTGATAAAAAAAGATTTGGCTGATATGATGCTTTTGCACCAGCACTTGTTCCAGTTGGTACGCCAGAAACGCGCCTTGACTACCGTAAACCGGTTGTTTca                                        >  1:438251/1‑250 (MQ=255)

                                                                                                                                                                                                             gCTATCCACCACGCCACGCCGTTGAGCGGCGGCAACGCACAAGTGCAGCGGCACGTTAAATTGACGGGAAAATGCCTGCCAGGCt                                                                                                                                                                                                           <  1:561940/85‑1 (MQ=255)

                                                                                                                                                                                                             gCTATCCACCACGCCACGCCGTTGAGCGGCGGCAACGCACAAGTGCAGCGGCACGTTAAATTGACGGGAAAATGCCTGCCAGGCTTGGACCAAATTAAATTCGTCATTAGCGGGATAAACCAAGGCGTTGCCGTTGGTTACGCCATCCTGATAAAAAAAGATTTGGCTGATATGATGCTTTTGCACCAGTACTTGTGCCAGTTGGTACGCCAGAAACGCGCCTTGACTACCGTAAACCGGTTGTTTcacca                                     >  1:147844/1‑251 (MQ=255)

                                                                                                                                                                                                             gCTATCCACCACGCCACGCCGTTGAGCGGCGGCAACGCACAAGTGCAGCGGCACGTTAAATTGACGGGAAAATGCCTGCCAGGCTTGGACCAAATTAAATTCGTCATTAGCGGGATAAACCAAGGCGTTGCCGTTGGTTACGCCATCCTGATAAAAAAAGATTTGGCTGATATGATGCTTTTGCACCAGTACTTGTGCCAGTTGGTACGCCAGAAACGCGCCTTGACTACCGTAAACCGGTTGTTTcacca                                     >  1:268966/1‑251 (MQ=255)

                                                                                                                                                                                                              cTATCCACCACGCCACGCCGTTGAGCGGCGGCAACGCACAAGTGCAGCGGCACGTTAAATTGACGGGAAAATGCCTGCCAGGCTTGGACCAAATTAAATTCGTCATTAGCGGGATAAACCAAGGCGTTGCCGTTGGTTACGCCATCCTGATAAAAAAAGATTTGGCTGATATGATGCTTTTGCACCAGTACTTGTGCCAGTTGGTACGCCAGAAACGCGCCTTGACTACCGTAAACCGGTTGTTTcaccac                                    <  1:45157/251‑1 (MQ=255)

                                                                                                                                                                                                              cTATCCACCACGCCACGCCGTTGAGCGGCGGCAACGCACAAGTGCAGCGGCACGTTAAATTGACGGGAAAATGCCTGCCAGGCTTGGACCAAATTAAATTCGTCATTAGCGGGATAAACCAAGGCGTTGCCGTTGGTTACGCCATCCTGATAAAAAAAGATTTGGCTGATATGATGCTTTTGCACCAGTACTTGTGCCAGTTGGTACGCCAGAAACGCGCCTTGACTACCGTAAACCGGTTGTTTcacca                                     >  1:199739/1‑250 (MQ=255)

                                                                                                                                                                                                                aTCCACCACGCCACGCCGTTGAGCGGCGGCAACGCACAAGTGCAGCGGCACGTTAAATTGACGGGAAAATGCCTGCCAGGCTTGGACCAAATTAAATTCGTCATTAGCGGGATAAACCAAGGCGTTGCCGTTGGTTACGCCATCCTGATAAAAAAAGATTTGGCTGATATGATGCTTTTGCACCAGTACTTGTGCCAGTTGGTACGCCAGAAACGCGCCTTGACTACCGTAAACCAGTTGTTTCAccacca                                  >  1:834009/1‑251 (MQ=255)

                                                                                                                                                                                                                 tCCACCACGCCACGCCGTTGAGCGGCGGCAACGCACAAGTGCAGCGGCACGTTAAATTGACGGGAAAATGCCTGCCAGGCTTGGACCAAATTAAATTCGTCATTAGCGGGATAAACCAAGGCGTTGCCGTTGGTTACGCCATCCTGATAAAAAAAGATTTGGCTGATATGATGCTTTTGCACCAGTACTTGTGCCAGTTGGTACGCCAGAAACGCGCCTTGACTACCGTAAACCGGTTGTTTCACCACCaa                                 <  1:709768/251‑1 (MQ=255)

                                                                                                                                                                                                                     ccacgccacgCCGTTGAGCGGCGGCAACGCACAAGTGCAGCGGCACGTTAAATTGACGGGAAAGTGCCTGCCAGGCTTGGACCAAATTAAATTCGTCATTAGCGGGATAAACCAAGGCGTTGCCGTTGGTTACGCCATCCTGATAAAAAAAGATTTGGCTGATATGATGCTTTTGCACCAGTACTTGTGCCAGTTGGTACGCCAGAAACGCGCCTTGACTACCGTAAACCGGTTGTTTCACCACCAacac                              <  1:884534/250‑1 (MQ=255)

                                                                                                                                                                                                                        cgccacgccGTTGAGCGGCGGCAACGCACAAGTGCAGCGGCACGTTAAATTGACGGGAAAATGCCTGCCAGGCTTGGACCAAATTAAATTCGTCATTAGCGGGATAAACCAAGGCGTTGCCGTTGGTTACGCCATCCTGATAAAAAAAGATTTGGCTGATATGATGCTTTTGCACCAGTACTTGTGCCAGTTGGTACGCCAGAAACGCGCCTTGACTACCGTAAACCGGTTGTTTCACCACCAACACATaa                          >  1:444321/1‑251 (MQ=255)

                                                                                                                                                                                                                          ccacgccGTTGAGCGGCGGCAACGCACAAGTGCAGCGGCACGTTAAATTGACGGGAAAATGCCTGCCAGGCTTGGACCAAATTAAATTCGTCATTAGCGGGATAAACCAAGGCGTTGCCGTTGGTTACGCCATCCTGATAAAAAAAGATTTGGCTGATATGATGCTTTTGCACCAGTACTTGTGCCAGTTGGTACGCCAGAAACGCGCCTTGACTACCGTAAACCGGTTGTTTCACCACCAACACATAATc                        <  1:678752/251‑1 (MQ=255)

                                                                                                                                                                                                                                cGTTGAGCGGCGGCAACGCACAAGTGCAGCGGCACGTTAAATTGACGGGAAAATGCCTGCCAGGCTTGGACCAAATTAAATTCGTCATTAGCGGGATAAACCAAGGCGTTGCCGTTGGTTACGCCATCCTGATAAAAAAAGATTTGGCTGATATGATGCTTTTGCACCAGTACTTGTGCCAGTTGGTACGCCAGAAACGCGCCTTGACTACCGTAAACCGGTTGTTTCACCACCAACACATAATCCATGAc                  <  1:592448/251‑1 (MQ=255)

                                                                                                                                                                                                                                cGTTGAGCGGCGGCAACGCACAAGTGCAGCGGCACGTTAAATTGACGGGAAAATGCCTGCCAGGCTTGGACCAAATTAAATTCGTCATTAGCGGGATAAACCAAGGCGTTGCCGTTGGTTACGCCATCCTGATAAAAAAAGATTTGGCTGATATGATGCTTTTGCACCAGTACTTGTGCCAGTTGGTACGCCAGAAACGCGCCTTGACTACCGTAAACCGGTTGTTTCACCACCAACACATAATCCATGAc                  <  1:810905/251‑1 (MQ=255)

                                                                                                                                                                                                                                cGTTGAGCGGCGGCAACGCACAAGTGCAGCGGCACGTTAAATTGACGGGAAAATGCCTGCCAGGCTTGGACCAAATTAAATTCGTCATTAGCGGGATAAACCAAGGCGTTGCCGTTGGTTACGCCATCCTGATAAAAAAAGATTTGGCTGATATGATGCTTTTGCACCAGTACTTGTGCCAGTTGGTACGCCAGAAACGCGCCTTGACTACCGTAAACCGGTTGTTTCACCACCAACACATAATCCATGAc                  <  1:665322/251‑1 (MQ=255)

                                                                                                                                                                                                                                 gTTGAGCGGCGGCAACGCACAAGTGCAGCGGCACGTTAAATTGACGGGAAAATGCCTGCCAGGCTTGGACCAAATTAAATTCGTCATTAGCGGGATAAACCAAGGCGTTGCCGTTGGTTACGCCATCCTGATAAAAAAAGATTTGGCTGATATGATGCTTTTGCAc                                                                                                      >  1:134321/1‑166 (MQ=255)

                                                                                                                                                                                                                                 gTTGAGCGGCGGCAACGCACAAGTGCAGCGGCACGTTAAATTGACGGGAAAATGCCTGCCAGGCTTGGACCAAATTAAATTCGTCATTAGCGGGATAAACCAAGGCGTTGCCGTTGGTTACGCCATCCTGATAAAAAAAGATTTGGCTGATATGATGCTTTTGCACCAGTACTTGTGCCAGTTGGTACGCCAGAAACGCGCCTTGACTACCGTAAACCGGTTGTTTCACCACCAACACATAATCCATGa                   >  1:302737/1‑249 (MQ=255)

                                                                                                                                                                                                                                      ccaacggcAACGCACAAGTGCAGCGGCACGTTAAATTGACGGGAAAATGCCTGCCAGGCTTGGACCAAATTAAATTCGTCATTAGCGGGATAAACCAAGGCGTTGCCGTTGGTTACGCCATCCTGATAAAAAAAGATTTGGCTGATATGATGCTTTTGCACCAGTACTTGTGCCAGTTGGTACGCCAGAAACGCGCCTTGACTACCGTAAACCGGTTGTTTCACCACCAACACATAATCCATGACTTATTc            <  1:569738/247‑1 (MQ=255)

                                                                                                                                                                                                                                         gcggcAACGCACAAGTGCAGCGGCACGTTAAATTGACGGGAAAATGCCTGCCAGGCTTGGACCAAATTAAATTCGTCATTAGCGGGATAAACCAAGGCGTTGCCGTTGGTTACGCCATCCTGATAAAAAAAGATTTGGCTGATATGATGCTTTTGCACCAGTACTTGTGCCAGTTGGTACGCCAGAAACGCGCCTTGACTACCGTAAACCGGTTGTTTCACCACCAACACATAATCCATGACTTATTCAt          >  1:657504/1‑250 (MQ=255)

                                                                                                                                                                                                                                             cAACGCACAAGTGCAGCGGCACGTTAAATTGACGGGAAAATGCCTGCCAGGCTTGGACCAAATTAAATTCGTCATTAGCGGGATAAACCAAGGCGTTGCCGTTGGTTACGCCATCCTGATAAAAAAAGATTTGGCTGATATGATGCTTTTGCACCAGTACTTGTGCCAGTTGGTACGCCAGAAACGCGCCTTGACTACCGTAAACCGGTTGTTTCACCACCAACACATAATCCATGAc                  >  1:637201/1‑238 (MQ=255)

                                                                                                                                                                                                                                              aaCGCACAAGTGCAGCGGCACGTTAAATTGACGGGAAAATGCCTGCCAGGCTTGGACCAAATTAAATTCGTCATTAGCGGGATAAACCAAGGCGTTGCCGTTGGTTACGCCATCCTGATAAAAAAAGATTTGGCTGATATGATGCTTTTGCACCAGTACTTGTGCCAGTTGGTACGCCAGAAACGCGCCTTGACTACCGTAAACCGGTTGTTTCACCACCAACACATAATCCATGACTTATTCATCCTGAt    <  1:97041/251‑1 (MQ=255)

                                                                                                                                                                                                                                               aCGCACAAGTGCAGCGGCACGTTAAATTGACGGGAAAATGCCTGCCAGGCTTGGACCAAATTAAATTCGTCATTAGCGGGATAAACCAAGGCGTTGCCGTTGGTTACGCCATCCTGATAAAAAAAGATTTGGCTGATATGATGCTTTTGCACCAGTACTTGTGCCAGTTGGTACGCCAGAAACGCGCCTTGACTACCGTAAACCGGTTGTTTCACCACCAACACATAATCCATGACTTATTCATCCTGATc   <  1:102954/251‑1 (MQ=255)

                                                                                                                                                                                                                                               aCGCACAAGTGCAGCGGCACGTTAAATTGACGGGAAAATGCCTGCCAGGCTTGGACCAAATTAAATTCGTCATTAGCGGGATAAACCAAGGCGTTGCCGTTGGTTACGCCATCCTGATAAAAAAAGATTTGGCTGATATGATGCTTTTGCACCAGTACTTGTGCCAGTTGGTACGCCAGAAACGCGCCTTGACTACCGTAAACCGGTTGTTTCACCACCAACACATAATCCATGACTTATTCATCCTGATc   <  1:737420/251‑1 (MQ=255)

                                                                                                                                                                                                                                                  cacaAGTGCAGCGGCACGTTAAATTGACGGGAAAATGCCTGCCAGGCTTGGACCAAATTAAATTCGTCATTAGCGGGATAAACCAAGGCGTTGCCGTTGGTTACGCCATCCTGATAAAAAAAGATTTGGCTGATATGATGCTTTTGCACCAGTACTTGTGCCAGTTGGTACGCCAGAAACGCGCCTTGACTACCGTAAACCGGTTGTTTCACCACCAACACATAATCCATGAc                  >  1:563919/1‑233 (MQ=255)

CGCAAAATGCCGTGGCGGCAAGCAGCGCGTCTAATCCTTCGCGTGTATTTGCCGAACCATAAGGCGCGTGACGGAAAATAAAGGCAAGTTTACTCATAGGGTTATCACCCGATCGGCTTGTAATAAGGCTTGGCTGAA‑TTCACCCAAGCCGGCAAGTTGAAAAACGTCCGCCAGGTTAACCGCACTTTTATCGGCGGCAGAATAGCTATCCACCACGCCACGCCGTTGAGCGGCGGCAACGCACAAATGCAGCGGCACGTTAAATTGACGGGAAAATGCCTGCCAGGCTTGGACCAAATTAAATTCGTCATTAGCGGGATAAACCAAGGCGTTGCCGTTGGTTACGCCATCCTGATAAAAAAAGATTTGGCTGATATGATGCTTTTGCACCAGTACTTGTGCCAGTTGGTACGCCAGAAACGCGCCTTGACTACCGTAAACCGGTTGTTTCACCACCAACACATAATCCATGACTTATTCATCCTGATCT  >  1/461981‑462470

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`