Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	446,542	T→C	intergenic (+614/‑164)	unknown → / → unknown	Uncharacterized protein YnbE/Integrase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	446,542	0	T	C	100.0%	210.1 / NA	73	intergenic (+614/‑164)	unknown/unknown	Uncharacterized protein YnbE/Integrase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (29/44);  total (29/44)

GCCTTCGCTCCCCAGCTTCCGCTCGTAAGACGGGGATAAAGCGGAGTCAAACCAAAACGAGCTCGTGTGGAAGCCACCGTTTGAGGATCGAAGCATTAAATTAAATCAAACTAGCTTAATTGTCGCGTGTCCGTCCGCAGGATTAAGTGAATTTAAAGACCGGACTAAACGTGTAGTGCTAACGGCAGAGGAATTTCGGACGGGGGTTCAACTCCCCCCAGCTCCACCAAA‑ACATAGTCCAAAGCAAACTTTACAAAACCTAAAAGCCCTTGAAAATATTAACCTCAAGGGCTTTTTTATTTCCTAAGCGTTCCTATAAGATACTACCAAAGCCTTGAATTTTAGTTATACGATTACCGCACTTTGGGCA  >  1/446308‑446677                                                                                                                                                                                                                                            |                                                                                                                                        gCCTTCGCTCCCCAGCTTCCGCTCGTAAGACGGGGATAAAGCGGAGTCAAACCAAAACGAGCTCGTGTGGAAGCCACCGTTTGAGGATCGAAGCATTAAATTAAATCAAACTAGCTTAATTGTCGCGTGTCCGTCCGCAGGATTAAGTGAATTTAAAGACCGGACTAAACGTGTAGTGCTAACGGCAGAGGAATTTCGGACGGGGGTTCAACTCCCCCCAGCTCCACCAAA‑TAACAAACCAAAGCAAACtt                                                                                                                         >  1:787763/1‑251 (MQ=255)     tCGCTCCCCAGCTTCCGCTCGTAAGACGGGGATAAAGCGGAGTCAAACCAAAACGAGCTCGTGTGGAAGCCACCGTTTGAGGATCGAAGCATTAAATTAAATCAAACTAGCTTAATTGTCGCGTGTCCGTCCGCAGGATTAAGTGAATTTAAAGACCGGACTAAACGTGTAGTGCTAACGGCAGAGGAATTTCGGACGGGGGTTCAACTCCCCCCAGCTCCACCAAA‑TAACAAACCAAAGCAAACttatca                                                                                                                     >  1:729390/1‑247 (MQ=255)        cTCCCCAGCTTCCGCTCGTAAGACGGGGATAAAGCGGAGTCAAACCAAAACGAGCTCGTGTGGAAGCCACCGTTTGAGGATCGAAGCATTAAATTAAATCAAACTAGCTTAATTGTCGCGTGTCCGTCCGCAGGATTAAGTGAATTTAAAGACCGGACTAAACGTGTAGTGCTAACGGCAGAGGAATTTCGGACGGGGGTTCAACTCCCCCCAGCTCCACCAAA‑TAACAAACCAAAGCAAACttatcaaat                                                                                                                  >  1:901799/1‑244 (MQ=255)        cTCCCCAGCTTCCGCTCGTAAGACGGGGATAAAGCGGAGTCAAACCAAAACGAGCTCGTGTGGAAGCCACCGTTTGAGGATCGAAGCATTAAATTAAATCAAACTAGCTTAATTGTCGCGTGTCCGTCCGCAGGATTAAGTGAATTTAAAGACCGGACTAAACGTGTAGTGCTAACGGCAGAGGAATTTCGGACGGGGGTTCAACTCCCCCCAGCTCCACCAAA‑TAACAAACCAAAGCAAACttatcaaa                                                                                                                   <  1:411572/250‑7 (MQ=255)                  tCCGCTCGTAAGACGGGGATAAAGCGGAGTCAAACCAAAACGAGCTCGTGTGGAAGCCACCGTTTGAGGATCGAAGCATTAAATTAAATCAAACTAGCTTAATTGTCGCGTGTCCGTCCGCAGGATTAAGTGAATTTAAAGACCGGACTAAACGTGTAGTGCTAACGGCAGAGGAATTTCGGACGGGGGTTCAACTCCCCCCAGCTCCACCAAA‑TAACAAACCAAAGCAAACTTATCAAATCCTAAAGGcc                                                                                                        <  1:19465/251‑1 (MQ=255)                        cGTAAGACGGGGATAAAGCGGAGTCAAACCAAAACGAGCTCGTGTGGAAGCCACCGTTTGAGGATCGAAGCATTAAATTAAATCAAACTAGCTTAATTGTCGCGTGTCCGTCCGCAGGATTAAGTGAATTTAAAGACCGGACTAAACGTGTAGTGCTAACGGCAGAGGAATTTCGGACGGGGGTTCAACTCCCCCCAGCTCCACCAAA‑TAACAAACCAAAGCAAACTTATCAAATCCTAAAAGCCCTTGaa                                                                                                  <  1:790520/251‑1 (MQ=255)                        cGTAAGACGGGGATAAAGCGGAGTCAAACCAAAACGAGCTCGTGTGGAAGCCACCGTTTGAGGATCGAAGCATTAAATTAAATCAAACTAGCTTAATTGTCGCGTGTCCGTCCGCAGGATTAAGAGAATTTAAAGACCGGACTAAACGTGTAGTGCTAACGGCAGAGGAATTTCGGACGGGGGTTCAACTCCCCCCAGCTCCACCAAA‑TAACAAACCAAAGCAAACTTATCAAATCCTAAAAGCCCTTGaa                                                                                                  >  1:353421/1‑251 (MQ=255)                          tAAGACGGGGATAAAGCGGAGTCAAACCAAAACGAGCTCGTGTGGAAGCCACCGTTTGAGGATCGAAGCATTAAATTAAATCAAACTAGCTTAATTGTCGCGTGTCCGTCCGCAGGATTAAGTGAATTTAAAGACCGGACTAAACGTGTAGTGCTAACGGCAGAGGAATTTCGGACGGGGGTTCAACTCCCCCCAGCTCCACCAAA‑TAACAAACCAAAGCAAACTTATCAAATCCTAAAAGCCCTTGaaag                                                                                                <  1:346129/251‑2 (MQ=255)                                 gggATAAAGCGGAGTCAAACCAAAACGAGCTCGTGTGGAAGCCACCGTTTGAGGATCGAAGCATTAAATTAAATCAAACTAGCTTAATTGTCGCGTGTCCGTCCGCAGGATTAAGTGAATTTAAAGACCGGACTAAACGTGTAGTGCTAACGGCAGAGGAATTTCGGACGGGGGTTCAACTCCCCCCAGCTCCACCAAA‑TAACAAACCAAAGCAAACTTATCAAATCCTAAAAGCCCTTGAAAGTATTaa                                                                                          >  1:846991/1‑250 (MQ=255)                                 gggATAAAGCGGAGTCAAACCAAAACGAGCTCGTGTGGAAGCCACCGTTTGAGGATCGAAGCATTAAATTAAATCAAACTAGCTTAATTGTCGCGTGTCCGTCCGCAGGATTAAGTGAATTTAAAGACCGGACTAAACGTGTAGTGCTAACGGCAGAGGAATTTCGGACGGGGGTTCAACTCCCCCCAGCTCCACCAAA‑TAACAAACCAAAGCAAACTTATCAAATCCTAAAAGCCCTTGAAAGTATTaa                                                                                          >  1:872162/1‑250 (MQ=255)                                  ggATAAAGCGGAGTCAAACCAAAACGAGCTCGTGTGGAAGCCACCGTTTGAGGATCGAAGCATTAAATTAAATCAAACTAGCTTAATTGTCGCGTGTCCGTCCGCAGGATTAAGTGAATTTAAAGACCGGACTAAACGTGTAGTGCTAACGGCAGAGGAATTTCGGACGGGGGTTCAACTCCCCCCAGCTCCACCAAA‑TAACAAACCAAAGCAAACTTATCAAATCCTAAAAGCCCTTGAAAGTATTAAc                                                                                         <  1:167563/250‑1 (MQ=255)                                    aTAAAGCGGAGTCAAACCAAAACGAGCTCGTGTGGAAGCCACCGTTTGAGGATCGAAGCATTAAATTAAATCAAACTAGCTTAATTGTCGCGTGTCCGTCCGCAGGATTAAGTGAATTTAAAGACCGGACTAAACGTGTAGTGCTAACGGCAGAGGAATTTCGGACGGGGGTTCAACTCCCCCCAGCTCCACCAAA‑TAACAAACCAAAGCAAACTTATCAAATCCTAAAAGCCCTTGAAAGTATTAActt                                                                                       <  1:315792/250‑3 (MQ=255)                                       aaGCGGAGTCAAACCAAAACGAGCTCGTGTGGAAGCCACCGTTTGAGGATCGAAGCATTAAATTAAATCAAACTAGCTTAATTGTCGCGTGTCCGTCCGCAGGATTAAGTGAATTTAAAGACCGGACTAAACGTGTAGTGCTAACGGCAGAGGAATTTCGGACGGGGGTTCAACTCCCCCCAGCTCCACCAAA‑TAACAAACCAAAGCAAACTTATCAAATCCTAAAAGCCCTTGAAAGTATTAActttaag                                                                                   <  1:43334/251‑7 (MQ=255)                                       aaGCGGAGTCAAACCAAAACGAGCTCGTGTGGAAGCCACCGTTTGAGGATCGAAGCATTAAATTAAATCAAACTAGCTTAATTGTCGCGTGTCCGTCCGCAGGATTAAGTGAATTTAAAGACCGGACTAAACGTGTAGTGCTAACGGCAGAGGAATTTCGGACGGGGGTTCAACTCCCCCCAGCTCCACCAAA‑TAACAAACCAAAGCAAACTTATCAAATCCTAAAAGCCCTTGAAAGTATTAActttaag                                                                                   <  1:402298/251‑7 (MQ=255)                                                cAAACCAAAACGAGCTCGTGTGGAAGCCACCGTTTGAGGATCGAAGCATTAAATTAAATCAAACTAGCTTAATTGTCGCGTGTCCGTCCGCAGGATTAAGTGAATTTAAAGACCGGACTAAACGTGTAGTGCTAACGGCAGAGGAATTTCGGACGGGGGTTC‑ACTCCCCCCAGCTCCACCAAA‑TAACAAACCAAAGCAAACTTATCAAATCCTAAAAGCCCTTGAAAGTATTAACTTTAAGGGCtttttat                                                                         <  1:374889/251‑3 (MQ=255)                                                 aaaCCAAAACGAGCTCGTGTGGAAGCCACCGTTTGAGGATCGAAGCATTAAATTAAATCAAACTAGCTTAATTGTCGCGTGTCCGTCCGCAGGATTAAGTGAATTTAAAGACCGGACTAAACGTGTAGTGCTAACGGCAGAGGAATTTCGGACGGGGGTTCAACTCCCCCCAGCTCCACCAAA‑TAACAAACCAAAGCAAACTTATCAAATCCTAAAAGCCCTTGAAAGTATTAACTTTAAGGGCtttttat                                                                         <  1:679841/251‑3 (MQ=255)                                                   aCCAAAACGAGCTCGTGTGGAAGCCACCGTTTGAGGATCGAAGCATTAAATTAAATCAAACTAGCTTAATTGTCGCGTGTCCGTCCGCAGGATTAAGTGAATTTAAAGACCGGACTAAACGTGTAGTGCTAACGGCAGAGGAATTTCGGACGGGGGTTCAACTCCCCCCAGCTCCACCAAA‑TAACAAACCAAAGCAAACttatcaaatc                                                                                                                 <  1:364728/209‑9 (MQ=255)                                                    ccAAAACGAGCTCGTGTGGAAGCCACCGTTTGAGGATCGAAGCATTAAATTAAATCAAACTAGCTTAATTGTCGCGTGTCCGTCCGCAGGATTAAGTGAATTTAAAGACCGGACTAAACGTGTAGTGCTAACGGCAGAGGAATTTCGGACGGGGGTTCAACTCCCCCCAGCTCCACCAAA‑TAACAAACCAAAGCAAACTTATCAAATCCTAAAAGCCCTTGAAAGTATTAACTTTAAGGGCtttttatatc                                                                      <  1:704843/251‑6 (MQ=255)                                                    ccAAAACGAGCTCGTGTGGAAGCCACCGTTTGAGGATCGAAGCATTAAATTAAATCAAACTAGCTTAATTGTCGCGTGTCCGTCCGCAGGATTAAGTGAATTTAAAGACCGGACTAAACGTGTAGTGCTAACGGCAGAGGAATTTCGGACGGGGGTTCAACTCCCCCCAGCTCCACCAAA‑TAACAAACCAAAGCAAACTTATCAAATCCTAAAAGCCCTTGAAAGTATTAACTTTAAGGGCtttttatat                                                                       >  1:387284/1‑246 (MQ=255)                                                     cAAAACGAGCTCGTGTGGAAGCCACCGTTTGAGGATCGAAGCATTAAATTAAATCAAACTAGCTTAATTGTCGCGTGTCCGTCCGCAGGATTAAGTGAATTTAAAGACCGGACTAAACGTGTAGTGCTAACGGCAGAGGAATTTCGGACGGGGGTTCAACTCCCCCCAGCTCCACCAAA‑TAACAAACCAAAGCAAACTTATCAAATCCTAAAAGCCCTTGAAAGTATTAACTTTAAGGGCtttttatatcc                                                                     <  1:502675/251‑7 (MQ=255)                                                     cAAAACGAGCTCGTGTGGAAGCCACCGTTTGAGGATCGAAGCATTAAATTAAATCAAACTAGCTTAATTGTCGCGTGTCCGTCCGCAGGATTAAGTGAATTTAAAGACCGGACTAAACGTGTAGTGCTAACGGCAGAGGAATTTCGGACGGGGGTTCAACTCCCCCCAGCTCCACCAAA‑TAACAAACCAAAGCAAACTTATCAAATCCTAAAAGCCCTTGAAAGTATTAACTTTAAGGGCtttttatatcc                                                                     <  1:602210/251‑7 (MQ=255)                                                       aaaCGAGCTCGTGTGGAAGCCACCGTTTGAGGATCGAAGCATTAAATTAAATCAAACTAGCTTAATTGTCGCGTGTCCGTCCGCAGGATTAAGTGAATTTAAAGACCGGACTAAACGTGTAGTGCTAACGGCAGAGGAATTTCGGACGGGGGTTCAAC‑CCCCCCAGCTCCACCAAA‑TAACAAACCAAAGCAAACTTATCAAATCCTAAAAGCCCTTGAAAGTATTAACTTTAAGGGCtttttatatcc                                                                     <  1:229110/248‑7 (MQ=255)                                                            aGCTCGTGTGGAAGCCACCGTTTGAGGATCGAAGCATTAAATTAAATCAAACTAGCTTAATTGTCGCGTGTCCGTCCGCAGGATTAAGTGAATTTAAAGACCGGACTAAACGTGTAGTGCTAACGGCAGAGGAATTTCGGACGGGGGTTCAACTCCCCCCAGCTCCACCAAA‑TAACAAACCAAAGCAAACTTATCAAATCCTAAAAGCCCTTGAAAGTATTAACTTTAAGGGC‑TTTTTATATCCTAAGCg                                                              <  1:398920/250‑1 (MQ=255)                                                              cTCGTGTGGAAGCCACCGTTTGAGGATCGAAGCATTAAATTAAATCAAACTAGCTTAATTGTCGCGTGTCCGTCCGCAGGATTAAGTGAATTTAAAGACCGGACTAAACGTGTAGTGCTAACGGCAGAGGAATTTCGGACGGGGGTTCAACTCCCCCCAGCTCCACCAAA‑TAACAAACCAAAGCAAACTTATCAAATCCTAAAAGCCCTTGAAAGTATTAACTTTAAGGGC‑TTTTTATATCCTAAGCGtt                                                            >  1:385821/1‑250 (MQ=255)                                                                 gtgtGGAAGCCACCGTTTGAGGATCGAAGCATTAAATTAAATCAAACTAGCTTAATTGTCGCGTGTCCGTCCGCAGGATTAAGTGAATTTAAAGACCGGACTAAACGTGTAGTGCTAACGGCAGAGGAATTTCGGACGGGGGTTCAACTCCCCCCAGCTCCACCAAA‑TAACAAACCAAAGCAAACTTATCAAATCCTAAAAGCCCTTGAAAGTATTAACTTTAAGGGC‑TTTTTATATCCTAAGCGTTCCta                                                        >  1:599406/1‑251 (MQ=255)                                                                    tgGAAGCCACCGTTTGAGGATCGAAGCATTAAATTAAATCAAACTAGCTTAATTGTCGCGTGTCCGTCCGCAGGATTAAGTGAATTTAAAGACCGGACTAAACGTGTAGTGCTAACGGCAGAGGAATTTCGGACGGGGGTTCAACTCCCCCCAGCTCCACCAAA‑TAACAAACCAAAGCATACTTATCAAATCCTAAAAGCCCTTGAAAGTATTAACTTTAAGGGC‑TTTTTATATCCTAAGCGTTCCtatcc                                                     <  1:642478/251‑3 (MQ=255)                                                                    tgGAAGCCACCGTTTGAGGATCGAAGCATTAAATTAAATCAAACTAGCTTAATTGTCGCGTGTCCGTCCGCAGGATTAAGTGAATTTAAAGACCGGACTAAACGTGTAGTGCTAACGGCAGAGGAATTTCGGACGGGGGTTCAACTCCCCCCAGCTCCACCAAA‑TAACAAACCAAAGCAAACTTATCAAATCCTAAAAGCCCTTGAAAGTATTAACTTTAAGGGC‑TTTTTATATCCTAAGCGTTCCtatcc                                                     <  1:121920/251‑3 (MQ=255)                                                                    tgGAAGCCACCGTTTGAGGATCGAAGCATTAAATTAAATCAAACTAGCTTAATTGTCGCGTGTCCGTCCGCAGGATTAAGTGAATTTAAAGACCGGACTAAACGTGTAGTGCTAACGGCAGAGGAATTTCGGACGGGGGTTCAACTCCCCCCAGCTCCACCAAA‑TAACAAACCAAAGCAAACTTATCAAATCCTAAAAGCCCTTGAAAGTATTAACTTTAAGGGC‑TTTTTATATCCTAAGCGTTCCtatcc                                                     <  1:702244/251‑3 (MQ=255)                                                                    tgGAAGCCACCGTTTGAGGATCGAAGCATTAAATTAAATCAAACTAGCTTAATTGTCGCGTGTCCGTCCGCAGGATTAAGTGAATTTAAAGACCGGACTAAACGTGTAGTGCTAACGGCAGAGGAATTTCGGACGGGGGTTCAACTCCCCCCAGCTCCACCAAA‑TAACAAACCAAAGCAAACTTATCAAATCCTAAAAGCCCTTGAAAGTATTAACTTTAAGGGC‑TTTTTATATCCTAAGCGTTCCtatcc                                                     <  1:548972/251‑3 (MQ=255)                                                                    tgGAAGCCACCGTTTGAGGATCGAAGCATTAAATTAAATCAAACTAGCTTAATTGTCGCGTGTCCGTCCGCAGGATTAAGTGAATTTAAAGACCGGACTAAACGTGTAGTGCTAACGGCAGAGGAACTTCGGACGGGGGTTCAACTCCCCCCAGCTCCACCAAA‑TAACAAACCAAAGCAAACTTATCAAATCCTAAAAGCCCTTGAAAGTATTAACTTTAAGGGC‑TTTTTATATCCTAAGCGTTCCtatcc                                                     <  1:859025/251‑3 (MQ=255)                                                                       aaGCCACCGTTTGAGGATCGAAGCATTAAATTAAATCAAACTAGCTTAATTGTCGCGTGTCCGTCCGCAGGATTAAGTGAATTTAAAGACCGGACTAAACGTGTAGTGCTAACGGCAGAGGAATTTCGGACGGGGGTTCAACTCCCCCCAGCTCCACCAAA‑TAACAAACCAAAGCAAACTTATCAAATCCTAAAAGCCCTTGAAAGTATTAACTTTAAGGGC‑TTTTTATATCCTAAGCGTTCCtatccaat                                                  <  1:796451/251‑6 (MQ=255)                                                                           cACCGTTTGAGGATCGAAGCATTAAATTAAATCAAACTAGCTTAATTGTCGCGTGTCCGTCCGCAGGATTAAGTGAATTTAAAGACCGGACTAAACGTGTAGTGCTAACGGCAGAGGAATTTCGGACGGGGGTTCAACTCCCCCCAGCTCCACCAAA‑TAACAAACCAAAGCAAACTTATCAAATCCTAAAAGCCCTTGAAAGTATTAACTTTAAGGGC‑TTTTTATATCCTAAGCg                                                              <  1:192630/235‑1 (MQ=255)                                                                            aCCGTTTGAGGATCGAAGCATTAAATTAAATCAAACTAGCTTAATTGTCGCGTGTCCGTCCGCAGGATTAAGTGAATTTAAAGACCGGACTAAACGTGTAGTGCTAACGGCAGAGGAATTTCGGACGGGGGTTCAACTCCCCCCAGCTCCACCAAA‑TAACAAACCAAAGCAAACTTATCAAATCCTAAAAGCCCTTGaa                                                                                                  >  1:457576/1‑199 (MQ=255)                                                                              cGTTTGAGGATCGAAGCATTAAATTAAATCAAACTAGCTTAATTGTCGCGTGTCCGTCCGCAGGATTAAGTGAATTTAAAGACCGGACTAAACGTGTAGTGCTAACGGCAGAGGAATTTCGGACGGGGGTTCAACTCCCCCCAGCTCCACCAAA‑TAACAAACCAAAGCAAACTTATCAAATCCTAAAAGCCCTTGAAAGTATTAACTTTAAGGGC‑TTTTTATATCCTAAGCGTTCCtatccaatcctataa                                           <  1:107541/251‑13 (MQ=255)                                                                               gTTTGAGGATCGAAGCATTAAATTAAATCAAACTAGCTTAATTGTCGCGTGTCCGTCCGCAGGATTAAGTGAATTTAAAGACCGGACTAAACGTGTAGTGCTAACGGCAGAGGAATTTCGGACGGGGGTTCAACTCCCCCCAGCTCCACCAAATAC‑CAAACCAAAGCAAACTTATCAAATCCTAAAAGCCCTTGAAAGTATTAACTTTAAGGGC‑TTTTTATATCCTAAGCGTTCCtatccaatcctataa                                           <  1:485932/250‑13 (MQ=255)                                                                                tttGAGGATCGAAGCATTAAATTAAATCAAACTAGCTTAATTGTCGCGTGTCCGTCCGCAGGATTAAGTGAATTTAAAGACCGGACTAAACGTGTAGTGCTAACGGCAGAGGAATTTCGGACGGGGGTTCAACTCCCCCCAGCTCCACCAAA‑TAACAAACCAAAGCAAACTTATCAAATCCTAAAAGCCCTTGAAAGTATTAACTTTAAGGGC‑TTTTTATATCCTAAGCGTTCCtatccaatcctataaaa                                         <  1:702802/251‑15 (MQ=255)                                                                                tttGAGGATCGAAGCATTAAATTAAATCAAACTAGCTTAATTGTCGCGTGTCCGTCCGCAGGATTAAGTGAATTTAAAGACCGGACTAAACGTGTAGTGCTAACGGCAGAGGAATTTCGGACGGGGGTTCAACTCCCCCCAGCTCCACCAAA‑TAACAAACCAAAGCAAACTTATCAAATCCTAAAAGCCCTTGAAAGTATTAACTTTAAGGGC‑TTTTTATATCCTAAGCGTTCCtatccaatcctataaaa                                         <  1:401771/251‑15 (MQ=255)                                                                                tttGAGGATCGAAGCATTAAATTAAATCAAACTAGCTTAATTGTCGCGTGTCCGTCCGCAGGATTAAGTGAATTTAAAGACCGGACTAAACGTGTAGTGCTAACGGCAGAGGAATTTCGGACGGGGGTTCAACTCCCCCCAGCTCCACCAAA‑TAACAAACCAAAGCAAACTTATCAAATCCTAAAAGCCCTTGAAAGTATTAACTTTAAGGGC‑TTTTTATATCCTAAGCGTTCCtatcc                                                     >  1:213460/1‑237 (MQ=255)                                                                                  tGAGGATCGAAGCATTAAATTAAATCAAACTAGCTTAATTGTCGCGTGTCCGTCCGCAGGATTAAGTGAATTTAAAGACCGGACTAAACGTGTAGTGCTAACGGCAGAGGAATTTCGGACGGGGGTTCAACTCCCCCCAGCTCCACCAAA‑TAACAAACCAAAGCAAACTTATCAAATCCTAAAAGCCCTTGAAAGTATTAACTTTAAGGGC‑TTTTTATATCCTAAGCGTTCCtatccaatcctataaaatc                                       <  1:703439/251‑17 (MQ=255)                                                                                           aaGCATTAAATTAAATCAAACTAGCTTAATTGTCGCGTGTCCGTCCGCAGGATTAAGTGAATTTAAAGACCGGACTAAACGTGTAGTGCTAACGGCAGAGGAATTTCGGACGGGGGTTCAACTCCCCCCAGCTCCACCAAA‑TAACAAACCAAAGCAAACTTATCAAATCCTAAAAGCCCTTGAAAGTATTAAct                                                                                        >  1:110349/1‑193 (MQ=255)                                                                                             gCATTAAATTAAATCAAACTAGCTTAATTGTCGCGTGTCCGTCCGCAGGATTAAGTGAATTTAAAGACCGGACTAAACGTGTAGTGCTAACGGCAGAGGAATTTCGGACGGGGGTTCAACTCCCCCCAGCTCCACCAAA‑TAACAAACCAAAGCAAACTTATCAAATCCTAAAAGCCCTTGAAAGTATTAActt                                                                                       >  1:875347/1‑191 (MQ=255)                                                                                             gCATTAAATTAAATCAAACTAGCTTAATTGTCGCGTGTCCGTCCGCAGGATTAAGTGAATTTAAAGACCGGACTAAACGTGTAGTGCTAACGGCAGAGGAATTTCGGACGGGGGTTCAACTCCCCCCAGCTCCACCAAA‑TAACAAACCAAAGCAAACTTATCAAATCCTAAAAGCCCTTGAAAGTATTAAc                                                                                         >  1:33879/1‑191 (MQ=255)                                                                                              cATTAAATTAAATCAAACTAGCTTAATTGTCGCGTGTCCGTCCGCAGGATTAAGTGAATTTAAAGACCGGACTAAACGTGTAGTGCTAACGGCAGAGGAATTTCGGACGGGGGTTCAACTCCCCCCAGCTCCACCAAA‑TAACAAACCAAAGCAAACTTATCAAATCCTAAAAGCCCTTGAAAGTATTAACTTTAAGGGC‑TTTTTATATCCTAAGCGTTCCTATCCAATCCTATAAAATCCTTGAATTTTAg                           <  1:450154/251‑1 (MQ=255)                                                                                                   aattaaatCAAACTAGCTTAATTGTCGCGTGTCCGTCCGCAGGATTAAGTGAATTTAAAGACCGGACTAAACGTGTAGTGCTAACGGCAGAGGAATTTCGGACGGGGGTTCAACTCCCCCCAGCTCCACCAAA‑TAACAAACCAAAGCAAACttatca                                                                                                                     <  1:858730/157‑5 (MQ=255)                                                                                                   aattaaatCAAACTAGCTTAATTGTCGCGTGTCCGTCCGCAGGATTAAGTGAATTTAAAGACCGGACTAAACGTGTAGTGCTAACGGCAGAGGAATTTCGGACGGGGGTTCAACTCCCCCCAGCTCCACCAAA‑TAACAAACCAAAGCAAACTTATCAAATCCTAAAAGCCCTTGAAAGTATTAACTTTAAGGGC‑TTTTTATATCCTAAGCGTTCCtatccaatcctataa                                           >  1:267982/1‑218 (MQ=255)                                                                                                       aaatCAAACTAGCTTAATTGTCGCGTGTCCGTCCGCAGGATTAAGTGAATTTAAAGACCGGACTAAACGTGTAGTGCTAACGGCAGAGGGATTTCGGACGGGGGTTCAACTCCCCCCAGCTCCACCAAA‑TAACAAACCAAAGCAAACTTATCAAATCCTAAAAGCCCTTGAAAGTATTAACTTTAAGGGCtttttatatcc                                                                     <  1:623468/201‑7 (MQ=255)                                                                                                        aatCAAACTAGCTTAATTGTCGCGTGTCCGTCCGCAGGATTAAGTGAATTTAAAGACCGGACTAAACGTGTAGTGCTAACGGCAGAGGAATTTCGGACGGGGGTTCAACTCCCCCCAGCTCCACCAAA‑TAACAAACCAAAGCAAACTTATCAAATCCTAAAAGCCCTTGAAAGTATTAACTTTAAGGGC‑TTTTTATATCCTAAGCGTTCCTATCCAATCCTATAAAATCCTTGAATTTTAGTTATACgttt                 >  1:547422/1‑248 (MQ=255)                                                                                                           cAAACTAGCTTAATTGTCGCGTGTCCGTCCGCAGGATTAAGTGAATTTAAAGACCGGACTAAACGTGTAGTGCTAACGGCAGAGGAATTTCGGACGGGGGTTCAACTCCCCCCAGCTCCACCAAA‑TAACAAACCAAAGCAAACTTATCAAATCCTAAAAGCCCTTGAAAGTATTAACTTTAAGGGC‑TTTTTATATCCTAAGCGTTCCTATCCAATCCTATAAAATCCTTGAATTTTAGTTATACGTTTagt              <  1:583234/251‑3 (MQ=255)                                                                                                                tAGCTTAATTGTCGCGTGTCCGTCCGCAGGATTAAGTGAATTTAAAGACCGGACTAAACGTGTAGTGCTAACGGCAGAGGAATTTCGGACGGGGGTTCAACTCCCCCCAGCTCCACCAAA‑TAACAAACCAAAGCAAACTTATCAAATCCTAAAAGCCCTTGAAAGTATTAACTTTAAGGGC‑TTTTTATATCCTAAGCGTTCCTATCCAATCCTATAAAATCCTTGAATTTTAGTTATACGTTTagttatac         <  1:479703/251‑8 (MQ=255)                                                                                                                tAGCTTAATTGTCGCGTGTCCGTCCGCAGGATTAAGTGAATTTAAAGACCGGACTAAACGTGTAGTGCTAACGGCAGAGGAATTTCGGACGGGGGTTCAACTCCCCCCAGCTCCACCAAA‑TAACAAACCAAAGCAAACTTATCAAATCCTAAAAGCCCTTGAAAGTATTAACTTTAAGGGC‑TTTTTATATCCTAAGCGTTCCTATCCAATCCTATAAAATCCTTGAATTTTAGTTATACGTTTagttatac         <  1:593805/251‑8 (MQ=255)                                                                                                                 aGCTTAATTGTCGCGTGTCCGTCCGCAGGATTAAGTGAATTTAAAGACCGGACTAAACGTGTAGTGCTAACGGCAGAGGAATTTCGGACGGGGGTTCAACTCCCCCCAGCTCCACCAAA‑TAACAAACCAAAGCAAACTTATCAAATCCTAAAAGCCCTTGAAAGTATTAACTTTAAGGGC‑TTTTTATATCCTAAGCGTTCCtatccaatcc                                                >  1:539839/1‑204 (MQ=255)                                                                                                                  gCTTAATTGTCGCGTGTCCGTCCGCAGGATTAAGTGAATTTAAAGACCGGACTAAACGTGTAGTGCTAACGGCAGAGGAATTTCGGACGGGGGTTCAACTCCCCCCAGCTCCACCAAA‑TAACAAACCAAAGCAAACTTATCAAATCCTAAAAGCCCTTGAAAGTATTAACTTTAAGGGC‑TTTTTATATCCTAAGCGTTCCTATCCAATCCTATAAAATCCTTGAATTTTAGTta                        <  1:448242/234‑1 (MQ=255)                                                                                                                     tAATTGTCGCGTGTCCGTCCGCAGGATTAAGTGAATTTAAAGACCGGACTAAACGTGTAGTGCTAACGGCAGAGGAATTTCGGACGGGGGTTCAACTCCCCCCAGCTCCACCAAA‑TAACAAACCAAAGCAAACTTATCAAATCCTAAAAGCCCTTGAAAGTATTAACTTTAAGGGC‑TTTTTATATCCTAAGCGTTCCtatccaatcc                                                >  1:333447/1‑200 (MQ=255)                                                                                                                     tAATTGTCGCGTGTCCGTCCGCAGGATTAAGTGAATTTAAAGACCGGACTAAACGTGTAGTGCTAACGGCAGAGGAATTTCGGACGGGGGTTCAACTCCCCCCAGCTCCACCAAA‑TAACAAACCAAAGCAAACTTATCAAATCCTAAAAGCCCTTGAAAGTATTAACTTTAAGGGC‑TTTTTATATCCTAAGCGTTCCTATCCAATCCTATAAAATCCTTGAATTTTAGTTATACGTTTagttatactgatc    <  1:741175/251‑13 (MQ=255)                                                                                                                       aTTGTCGCGTGTCCGTCCGCAGGATTAAGTGAATTTAAAGACCGGACTAAACGTGTAGTGCTAACGGCAGAGGAATTTCGGACGGGGGTTCAACTCCCCCCAGCTCCACCAAA‑TAACAAACCAAAGCAAACTTATCAAATCCTAAAAGCCCTTGAAAGTATTAACTTTAAGGGC‑TTTTTATATCCTAAGCGTTCCTATCCAATCCTATAAAATCCTTGAATTTTAGTTATACGTTTagttatactgatagc  <  1:741132/251‑15 (MQ=255)                                                                                                                           tCGCGTGTCCGTCCGCAGGATTAAGTGAATTTAAAGACCGGACTAAACGTGTAGTGCTAACGGCAGAGGAATTTCGGACGGGGGTTCAACTCCCCCCAGCTCCACCAAA‑TAACAAACCAAAGCAAACTTATCAAATCCTAAAAGCCCTTGAAAGTATTAACTTTAAGGGC‑TTTTTATATCCTAAGCGTTCCTATCCAATCCTATAAAATCCTTGAATTTTAGTTATACg                    >  1:885298/1‑229 (MQ=255)                                                                                                                               gtgtCCGTCCGCAGGATTAAGTGAATTTAAAGACCGGACTAAACGTGTAGTGCTAACGGCAGAGGAATTTCGGACGGGGGTTCAACTCCCCCCAGCTCCACCAAA‑TAACAAACCAAAGCAAACTTATCAAATCCTAAAAGCCCTTGAAAGTATTAACTTTAAGGGC‑TTTTTATATCCTAAGCGTTCCTATCCAATCCTATAAAATCCTTGAATTTTAGTTATACGTTTagttatactg       >  1:159848/1‑229 (MQ=255)                                                                                                                               gtgtCCGTCCGCAGGATTAAGTGAATTTAAAGACCGGACTAAACGTGTAGTGCTAACGGCAGAGGAATTTCGGACGGGGGTTCAACTCCCCCCAGCTCCACCAAA‑TAACAAACCAAAGCAAACTTATCAAATCCTAAAAGCCCTTGAAAGTATTAACTTTAAGGGC‑TTTTTATATCCTAAGCGTTCCTATCCAATCCTATAAAATCCTTGAATTTTAGTTATACGTTTagttatac         >  1:257305/1‑229 (MQ=255)                                                                                                                                tgtCCGTCCGCAGGATTAAGTGAATTTAAAGACCGGACTAAACGTGTAGTGCTAACGGCAGAGGAATTTCGGACGGGGGTTCAACTCCCCCCAGCTCCACCAAA‑TAACAAACCAAAGCAAACTTATCAAATCCTAAAAGCCCTTGAAAGTATTAAc                                                                                         >  1:603038/1‑156 (MQ=255)                                                                                                                                tgtCCGTCCGCAGGATTAAGTGAATTTAAAGACCGGACTAAACGTGTAGTGCTAACGGCAGAGGAATTTCGGACGGGGGTTCAACTCCCCCCAGCTCCACCAAA‑TAACAAACCAAAGCAAACTTATCAAATCCTAAAAGCCCTTGAAAGTATTAACTTTAAGGGC‑TTTTTATATCCTAAGCGTTCCtatccaatcc                                                <  1:311278/196‑8 (MQ=255)                                                                                                                                       ccgCAGGATTAAGTGAATTTAAAGACCGGACTAAACGTGTAGTGCTAACGGCAGAGGAATTTCGGACGGGGGTTCAACTCCCCCCAGCTCCACCAAA‑TAACAAACCAAAGCAAACTTATCAAATCCTAAAAGcc                                                                                                        >  1:298908/1‑134 (MQ=255)                                                                                                                                            ggATTAAGTGAATTTAAAGACCGGACTAAACGTGTAGTGCTAACGGCAGAGGAATTTCGGACGGGGGTTCAACTCCCCCCAGCTCCACCAAA‑TAACAAACCAAAGCAAACTTATCAAATCCTAAAAGCCCTTGAAAGTATTAACTTTAAGGGCttttta                                                                          >  1:179831/1‑158 (MQ=255)                                                                                                                                            ggATTAAGTGAATTTAAAGACCGGACTAAACGTGTAGTGCTAACGGCAGAGGAATTTCGGACGGGGGTTCAACTCCCCCCAGCTCCACCAAA‑TAACAAACCAAAGCAAACTTATCAAATCCTAAAAGCCCTTGAAAGTATTAACTTTAAGGGC‑TTTTTATATCCTAAGCGTTCCTATCCAATCCTATAAAATCCTTGAATTTTAGTTATACGTTTagttatactg       >  1:46508/1‑216 (MQ=255)                                                                                                                                             gATTAAGTGAATTTAAAGACCGGACTAAACGTGTAGTGCTAACGGCAGAGGAATTTCGGACGGGGGTTCAACTCCCCCCAGCTCCACCAAA‑TAACAAACCAAAGCAAACTTATCAAATCCTAAAAGCCCTTGAAAGTATTAACTTTAAGGGC‑TTTTTATATCCTAAGCGTTCCtatccaatcctataaaa                                         <  1:11837/190‑15 (MQ=255)                                                                                                                                                  aGTGAATTTAAAGACCGGACTAAACGTGTAGTGCTAACGGCAGAGGAATTTCGGACGGGGGTTCAACTCCCCCCAGCTCCACCAAA‑TAACAAACCAAAGCAAACTTATCAAATCCTAAAAGCCCTTGAAAGTATTAACTTTAAGGGCtttttatatcc                                                                     <  1:629473/158‑7 (MQ=255)                                                                                                                                                                   gACTAAACGTGTAGTGCTAACGGCAGAGGAATTTCGGACGGGGGTTCAACTCCCCCCAGCTCCACCAAA‑TAACAAACCAAAGCAAACTTATCAAATCCTAAAAGCCCTTGAAAGTATTAACTTTAAGGGC‑TTTTTATATCCTAAGCGTTCCTATCCAATCCTATAAAATCCTTGAATTTTAGTTATACGTTTagttatac         >  1:98202/1‑193 (MQ=255)                                                                                                                                                                        aaCGTGTAGTGCTAACGGCAGAGGAATTTCGGACGGGGGTTCAACTCCCCCCAGCTCCACCAAA‑TAACAAACCAAAGCAAACTTATCAAATCCTAAAAGCCCTTGAAAGTATTAACTTTAAGGGC‑TTTTTATATCCTAAGCGTTCCtatccaatcc                                                <  1:234182/156‑8 (MQ=255)                                                                                                                                                                        aaCGTGTAGTGCTAACGGCAGAGGAATTTCGGACGGGGGTTCAACTCCCCCCAGCTCCACCAAA‑TAACAAACCAAAGCAAACTTATCAAATCCTAAAAGCCCTTGAAAGTATTAACTTTAAGGGC‑TTTTTATATCCTAAGCGTTCCtatcc                                                     >  1:471394/1‑149 (MQ=255)                                                                                                                                                                          cGTGTAGTGCTAACGGCAGAGGAATTTCGGACGGGGGTTCAACTCCCCCCAGCTCCACCAAA‑TAACAAACCAAAGCAAACTTATCAAATCCTAAAAGCCCTTGAAAGTATTAACTTTAAGGGCtttttatatcc                                                                     >  1:806771/1‑128 (MQ=255)                                                                                                                                                                           gtgtAGTGCTAACGGCAGAGGAATTTCGGACGGGGGTTCAACTCCCCCCAGCTCCACCAAA‑TAACAAACCAAAGCAAACTTATCAAATCCTAAAAGCCCTTGAAAGTATTAACTTTAAGGGC‑TTTTTATATCCTAAGCGTTCCTATCCAATCCTATAAAATCCTTGAATTTTAGTTATACGTTTagttatac         <  1:3565/192‑8 (MQ=255)                                                                                                                                                                               aGTGCTAACGGCAGAGGAATTTCGGACGGGGGTTCAACTCCCCCCAGCTCCACCAAA‑TAACAAACCAAAGCAAACTTATCAAATCCTAAAAGCCCTTGAAAGTATTAACTTTAAGGGCtttttatatc                                                                      >  1:507417/1‑123 (MQ=255)                                                                                                                                                                                gTGCTAACGGCAGAGGAATTTCGGACGGGGGTTCAACTCCCCCCAGCTCCACCAAA‑TAACAAACCAAAGCAAACTTATCAAATCCTAAAAGCCCTTGaaa                                                                                                 <  1:660027/100‑1 (MQ=255)                                                                                                                                                                                                  tttCGGACGGGGGTTCAACTCCCCCCAGCTCCACCAAA‑TAACAAACCAAAGCAAACTTATCAAATCCTAAAAGCCCTTGAAAGTATTAACTTTAAGGGC‑TTTTTATATCCTAAGCGTTCCtat                                                       <  1:241467/123‑1 (MQ=255)                                                                                                                                                                                                                                            |                                                                                                                                        GCCTTCGCTCCCCAGCTTCCGCTCGTAAGACGGGGATAAAGCGGAGTCAAACCAAAACGAGCTCGTGTGGAAGCCACCGTTTGAGGATCGAAGCATTAAATTAAATCAAACTAGCTTAATTGTCGCGTGTCCGTCCGCAGGATTAAGTGAATTTAAAGACCGGACTAAACGTGTAGTGCTAACGGCAGAGGAATTTCGGACGGGGGTTCAACTCCCCCCAGCTCCACCAAA‑ACATAGTCCAAAGCAAACTTTACAAAACCTAAAAGCCCTTGAAAATATTAACCTCAAGGGCTTTTTTATTTCCTAAGCGTTCCTATAAGATACTACCAAAGCCTTGAATTTTAGTTATACGATTACCGCACTTTGGGCA  >  1/446308‑446677

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑