Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	401,447	G→A	S26N (AGC→AAC)	unknown →	putative membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	401,447	0	G	A	100.0%	221.6 / NA	75	S26N (AGC→AAC)	unknown	putative membrane protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (34/41);  total (34/41)

CGGAGCGGTTGTTGGTGACAAAAGAAAATTTAGAAGAATAAAAGTGCGGTGGATTTTTAATGTGTTTTTACAATTTCCCCATGATGTTAAAAAAACGTTGAATTTTAGGCGTAAAACAGTTAATTTTTCCGCCTTTTATACTTTTATTTTTTATTGGGGGAAACCGTGCAACAAGCGAACCAAGGACAAC‑AAATTAAACCGCGTTATGAGCCGGCGGCAATCAGTAAAATTATTTTCGCCAGCCGTTGGCTGCAACTGCCGATTTATCTCGGGTTGATTATCGTGCAAGGCATTTATGCCTATAAATTTATGAAATCCCTGTGGCATTTAATTGTCAATTTACAGGAAATGGATTCCAACACCGTGATGTTGGCGGTATTGAATCTGATTGATGTTGTCATGATCGCCAATTTGCTGGTGATGGTGATTATCGGCGGGTATGAGATTTTCGTCTCCAAGCTGCGCACGCGTGGGCAT  >  1/401206‑401682                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                            cGGAGCGGTTGTTGGTGACAAAAGAAAATTTAGAAGAATAAAAGTGCGGTGGATTTTTAATGTGTTTTTACAATTTCCCCATGATGTTAAAAAAACGTTGAATTTTAGGCGTAAAACAGTTAATTTTTCCGCCTTTTATACTTTTATTTTTTATTGGGGGAAACCGTGCAACAAGCGAACCAAGGACAAC‑AAATTAAACCGCGTTATGAGCCGGCGGCAATCAGTAAAATTATTTTCGCCAACCGTTGGCt                                                                                                                                                                                                                                    <  1:244714/251‑1 (MQ=255)   gAGCGGTTGTTGGTGACAAAAGAAAATTTAGAAGAATAAAAGTGCGGTGGATTTTTAATGTGTTTTTACAATTTCCCCATGATGTTAAAAAAACGTTGAATTTTAGGCGTAAAACAGTTAATTTTTCCGCCTTTTATACTTTTATTTTTTATTGGGGGAAACCGTGCAACAAGCGAACCAAGGACAAC‑AAATTAAACCGCGTTATGAGCCGGCGGCAATCAGTAAAATTATTTTCGCCAACCGTTGGCTg                                                                                                                                                                                                                                   <  1:105002/250‑1 (MQ=255)      cGGTTGTTGGTGACAAAAGAAAATTTAGAAGAATAAAAGTGCGGTGGATTTTTAATGTGTTTTTACAATTTCCCCATGATGTTAAAAAAACGTTGAATTTTAGGCGTAAAACAGTTAATTTTTCCGCCTTTTATACTTTTATTTTTTATTGGGGGAAACCGTGCAACAAGCGAACCAAGGACAAC‑AAATTAAACCGCGTTATGAGCCGGCGGCAATCAGTAAAATTATTTTCGCCAACCGTTGGCTGCAAc                                                                                                                                                                                                                               <  1:707951/251‑1 (MQ=255)                    aaaaGAAAATTTAGAAGAATAAAAGTGCGGTGGATTTTTAATGTGTTTTTACAATTTCCCCATGATGTTAAAAAAACGTTGAATTTTAGGCGTAAAACAGTTAATTTTTCCGCCTTTTATACTTTTATTTTTTATTGGGGGAAACCGTGCAACAAGCGAACCAAGGACAAC‑AAATTAAACCGCGTTATGAGCCGGCGGCAATCAGTAAAATTATTTTCGCCAACCGTTGGCTGCAACTGCCGATTTAtctc                                                                                                                                                                                                                 <  1:93425/251‑1 (MQ=255)                     aaaGAAAATTTAGAAGAATAAAAGTGCGGTGGATTTTTAATGTGTTTTTACAATTTCCCCATGATGTTAAAAAAACGTTGAATTTTAGGCGTAAAACAGTTAATTTTTCCGCCTTTTATACTTTTATTTTTTATTGGGGGAAACCGTGCAACAAGCGAACCAAGGACAAC‑AAATTAAACCGCGTTATGAGCCGGCGGCAATCAGTAAAATTATTTTCGCCAACCGTTGGCTGCAACTGCCGATTTATCTCg                                                                                                                                                                                                                >  1:362229/1‑251 (MQ=255)                               tAGAAGAATAAAAGTGCGGTGGATTTTTAATGTGTTTTTACAATTTCCCCATGATGTTAAAAAAACGTTGAATTTTAGGCGTAAAACAGTTAATTTTTCCGCCTTTTATACTTTTATTTTTTATTGGGGGAAACCGTGCAACAAGCGAACCAAGGACAAC‑AAATTAAACCGCGTTATGAGCCGGCGGCAATCAGTAAAATTATTTTCGCCAACCGTTGGCTGCAACTGCCGATTTATCTCGGGTTGATTAt                                                                                                                                                                                                      <  1:582127/251‑1 (MQ=255)                               tAGAAGAATAAAAGTGCGGTGGATTTTTAATGTGTTTTTACAATTTCCCCATGATGTTAAAAAAACGTTGAATTTTAGGCGTAAAACAGTTAATTTTTCCGCCTTTTATACTTTTATTTTTTATTGGGGGAAACCGTGCAACAAGCGAACCAAGGACAAC‑AAATTAAACCGCGTTATGAGCCGGCGGCAATCAGTAAAATTATTTTCGCCAACCGTTGGCTGCAACTGCCGATTTATCTCGGGTTGATTAt                                                                                                                                                                                                      <  1:576803/251‑1 (MQ=255)                                    gaaTAAAAGTGCGGTGGATTTTTAATGTGTTTTTACAATTTCCCCATGATGTTAAAAAAACGTTGAATTTTAGGCGTAAAACAGTTAATTTTTCCGCCTTTTATACTTTTATTTTTTATTGGGGGAAACCGTGCAACAAGCGAACCAAGGACAAC‑AAATTAAACCGCGTTATGAGCCGGCGGCAATCAGTAAAATTATTTTCGCCAACCGTTGGCTGCAACTGCCGAtt                                                                                                                                                                                                                       >  1:545537/1‑229 (MQ=255)                                    gaaTAAAAGTGCGGTGGATTTTTAATGTGTTTTTACAATTTCCCCATGATGTTAAAAAAACGTTGAATTTTAGGCGTAAAACAGTTAATTTTTCCGCCTTTTATACTTTTATTTTTTATTGGGGGAAACCGTGCAACAAGCGAACCAAGGACAAC‑AAATTAAACCGCGTTATGAGCCGGCGGCAATCAGTAAAATTATTTTCGCCAACCGTTGGCTGCAACTGCCGAtt                                                                                                                                                                                                                       <  1:297412/229‑1 (MQ=255)                                     aaTAAAAGTGCGGTGGATTTTTAATGTGTTTTTACAATTTCCCCATGATGTTAAAAAAACGTTGAATTTTAGGCGTAAAACAGTTAATTTTTCCGCCTTTTATACTTTTATTTTTTATTGGGGGAAACCGTGCAACAAGCGAACCAAGGACAAC‑AAATTAAACCGCGTTATGAGCCGGCGGCAATCAGTAAAATTATTTTCGCCAACCGTTGGCTGCAACTGCCGATTTATCTCGGGTTGATTATCGTGCa                                                                                                                                                                                                >  1:337433/1‑251 (MQ=255)                                      aTAAAAGTGCGGTGGATTTTTAATGTGTTTTTACAATTTCCCCATGATGTTAAAAAAACGTTGAATTTTAGGCGTAAAACAGTTAATTTTTCCGCCTTTTATACTTTTATTTTTTATTGGGGGAAACCGTGCAACAAGCGAACCAAGGACAAC‑AAATTAAACCGCGTTATGAGCCGGCGGCAATCAGTAAAATTATTTTCGCCAACCGTTGGCTGCAACTGCCGATTTATCTCGGGTTGATTATCGTGCaa                                                                                                                                                                                               >  1:112464/1‑251 (MQ=255)                                          aaGTGCGGTGGATTTTTAATGTGTTTTTACAATTTCCCCATGATGTTAAAAAAACGTTGAATTTTAGGCGTAAAACAGTTAATTTTTCCGCCTTTTATACTTTTATTTTTTATTGGGGGAAACCGTGCAACAAGCGAACCAAGGACAAC‑AAATTAAACCGCGTTATGAGCCGGCGGCAATCAGTAAAATTATTTTCGCCAACCGTTGGCTGCAACTGCCGATTTATCTCGGGTTGATTATCGTGCAAGGCa                                                                                                                                                                                           <  1:492419/251‑1 (MQ=255)                                                 gTGGATTTTTAATGTGTTTTTACAATTTCCCCATGATGTTAAAAAAACGTTGAATTTTAGGCGTAAAACAGTTAATTTTTCCGCCTTTTATACTTTTATTTTTTATTGGGGGAAACCGTGCAACAAGCGAACCAAGGACAAC‑AAATTAAACCGCGTTATGAGCCGGCGGCAATCAGTAAAATTATTTTCGCCAACCGTTGGCTGCAACTGCCGATTTATCTCGGGTTGATTATCGTGCAAGGCATTTATg                                                                                                                                                                                     <  1:392800/250‑1 (MQ=255)                                                 gTGGATTTTTAATGTGTTTTTACAATTTCCCCATGATGTTAAAAAAACGTTGAATTTTAGGCGTAAAACAGTTAATTTTTCCGCCTTTTATACTTTTATTTTTTATTGGGGGAAACCGTGCAACAAGCGAACCAAGGACAAC‑AAATTAAACCGCGTTATGAGCCGGCGGCAATCAGTAAAATTATTTTCGCCAACCGTTGGCTGCAACTGCCGATTTATCTCGGGTTGATTATCGTGCAAGGCATTTATg                                                                                                                                                                                     <  1:850079/250‑1 (MQ=255)                                                      tttttAATGTGTTTTTACAATTTCCCCATGATGTTAAAAAAACGTTGAATTTTAGGCGTAAAACAGTTGATTTTTCCGCCTTTTATACTTTTATTTTTTATTGGGGGAAACCGTGCAACAAGCGAACCAAGGACAAC‑AAATTAAACCGCGTTATGAGCCGGCGGCAATCAGTAAAATTATTTTCGCCAACCGTTGGCTGCAACTGCCGATTTATCTCGGGTTGAtt                                                                                                                                                                                                        >  1:738583/1‑226 (MQ=255)                                                         ttAATGTGTTTTTACAATTTCCCCATGATGTTAAAAAAACGTTGAATTTTAGGCGTAAAACAGTTAATTTTTCCGCCTTTTATACTTTTATTTTTTATTGGGGGAAACCGTGCAACAAGCGAACCAAGGACAAC‑AAATTAAACCGCGTTATGAGCCGGCGGCAATCAGTAAAATTATTTTCGCCAACCGTTGGCTGCAACTGCCGATTTATCTCGGGTTGATTATCGTGCAAGGCATTTATGCCTATAAAt                                                                                                                                                                            <  1:497668/251‑1 (MQ=255)                                                                gtTTTTACAATTTCCCCATGATGTTAAAAAAACGTTGAATTTTAGGCGTAAAACAGTTAATTTTTCCGCCTTTTATACTTTTATTTTTTATTGGGGGAAACCGTGCAACAAGCGAACCAAGGACAAC‑AAATTAAACCGCGTTATGAGCCGGCGGCAATCAGTAAAATTATTTTCGCCAACCGTTGGCTGCAACTGCCGATTTATCTCGGGTTGATTATCGTGCAAGGCATTTATGCCTATAAATTTATGaa                                                                                                                                                                     >  1:245434/1‑251 (MQ=255)                                                                       cAATTTCCCCATGATGTTAAAAAAACGTTGAATTTTAGGCGTAAAACAGTTAATTTTTCCGCCTTTTATACTTTTATTTTTTATTGGGGGAAACCGTGCAACAAGCGAACCAAGGACAAC‑AAATTAAACCGCGTTATGAGCCGGCGGCAATCAGTAAAATTATTTTCGCCAACCGTTGGCTGCAACTGCCGATTTATCTCGGGTTGATTATCGTGCAAGGCATTTATGCCTATAAATTTATGAAATcc                                                                                                                                                                 >  1:601674/1‑248 (MQ=255)                                                                           ttCCCCATGATGTTAAAAAAACGTTGAATTTTAGGCGTAAAACAGTTAATTTTTCCGCCTTTTATACTTTTATTTTTTATTGGGGGAAACCGTGCAACAAGCGAACCAAGGACAAC‑AAATTAAACCGCGTTATGAGCCGGCGGCAATCAGTAAAATTATTTTCGCCAACCGTTGGCTGCAACTGCCGATTTATCTCGGGTTGATTATCGTGCAAGGCATTTATGCCTATAAATTTATGAAATCCCTGTgg                                                                                                                                                           >  1:795093/1‑250 (MQ=255)                                                                                  tgatgTTAAAAAAACGTTGAATTTTAGGCGTAAAACAGTTAATTTTTCCGCCTTTTATACTTTTATTTTTTATTGGGGGAAACCGTGCAACAAGCGAACCAAGGACAAC‑AAATTAAACCGCGTTATGAGCCGGCGGCAATCAGTAAAATTATTTTCGCCAACCGTTGGCTGCAACTGCCGATTTATCTCGGGTTGATTATCGTGCAAGGCATTTATGCCTATAAATTTATGAAATCCCTGTGGCATTTAAt                                                                                                                                                   <  1:356076/251‑1 (MQ=255)                                                                                                        tttAGGCGTAAAACAGTTAATTTTTCCGCCTTTTATACTTTTATTTTTTATTGGGGGAAACCGTGCAACAAGCGAACCAAGGACAAC‑AAATTAAACCGCGTTATGAGCCGGCGGCAATCAGTAAAATTATTTTCGCCAACCGTTGGCTGCAACTGCCGATTTATCTCGGGTTGATTATCGTGCAAGGCATTTATGCCTATAAATTTATGAAATCCCTGTGGCATTTAATTGTCAATTTACAGGAAATGGAt                                                                                                                             <  1:280169/251‑1 (MQ=255)                                                                                                             gCGTAAAACAGTTAATTTTTCCGCCTTTTATACTTTTATTTTTTATTGGGGGAAACCGTGCAACAAGCGAACCAAGGACAAC‑AAATTAAACCGCGTTATGAGCCGGCGGCAATCAGTAAAATTATTTTCGCCAACCGTTGGCTGCAACTGCCGATTTAtct                                                                                                                                                                                                                  <  1:827476/161‑1 (MQ=255)                                                                                                             gCGTAAAACAGTTAATTTTTCCGCCTTTTATACTTTTATTTTTTATTGGGGGAAACCGTGCAACAAGCGAACCAAGGACAAC‑AAATTAAACCGCGTTATGAGCCGGCGGCAATCAGTAAAATTATTTTCGCCAACCGTTGGCTGCAACTGCCGATTTATCTCGGGTTGATTATCGTGCAAGGCATTTATg                                                                                                                                                                                     <  1:709360/190‑1 (MQ=255)                                                                                                             gCGTAAAACAGTTAATTTTTCCGCCTTTTATACTTTTATTTTTTATTGGGGGAAACCGTGCAACAAGCGAACCAAGGACAAC‑AAATTAAACCGCGTTATGAGCCGGCGGCAATCAGTAAAATTATTTTCGCCAACCGTTGGCTGCAACTGCCGATTTATCTCGGGTTGATTATCGTGCAAGGCATTTATGCCTATAAATTTATGAAATCCCTGTGGCATTTAATTGTCAATTTACAGGAAATGGATTcc                                                                                                                          <  1:216782/249‑1 (MQ=255)                                                                                                             gCGTAAAACAGTTAATTTTTCCGCCTTTTATACTTTTATTTTTTATTGGGGGAAACCGTGCAACAAGCGAACCAAGGACAAC‑AAATTAAACCGCGTTATGAGCCGGCGGCAATCAGTAAAATTATTTTCGCCAACCGTTGGCTGCAACTGCCGATTTATCTCGGGTTGATTATCGTGCAAGGCATTTATGCCTATAAATTTATGAAATCCCTGTGGCATTTAATTGTCAATTTACAGGAAATGGATTCCaa                                                                                                                        >  1:167388/1‑251 (MQ=255)                                                                                                                tAAAACAGTTAATTTTTCCGCCTTTTATACTTTTATTTTTTATTGGGGGAAACCGTGCAACAAGCGAACCAAGGACAAC‑AAATTAAACCGCGTTATGAGCCGGCGGCAATCAGTAAAATTATTTTCGCCAACCGTTGGCTGCAACTGCCGATTTATCTCGGGTTGATTATCGTGCAAGGCATTTATGCCTATAAATTTATGAAATCCCTGTGGCATTTAATTGTCAATTTACAGGAAATGGATTCCAacac                                                                                                                     <  1:125591/251‑1 (MQ=255)                                                                                                                tAAAACAGTTAATTTTTCCGCCTTTTATACTTTTATTTTTTATTGGGGGAAACCGTGCAACAAGCGAACCAAGGACAAC‑AAATTAAACCGCGTTATGAGCCGGCGGCAATCAGTAAAATTATTTTCGCCAACCGTTGGCTGCAACTGCCGATTTATCTCGGGTTGATTATCGTGCAAGGCATTTATGCCTATAAATTTATGAAATCCCTGTGGCATTTAATTGTCAATTTACAGGAAATGGATTCCAacac                                                                                                                     <  1:731465/251‑1 (MQ=255)                                                                                                                     cAGTTAATTTTTCCGCCTTTTATACTTTTATTTTTTATTGGGGGAAACCGTGCAACAAGCGAACCAAGGACAAC‑AAATTAAACCGCGTTATGAGCCGGCGGCAATCAGTAAAATTATTTTCGCCAACCGTTGGCTGCAACTGCCGATTTATCTCGGGTTGATTATCGTGCAAGGCATTTa                                                                                                                                                                                       <  1:608202/180‑1 (MQ=255)                                                                                                                     cAGTTAATTTTTCCGCCTTTTATACTTTTATTTTTTATTGGGGGAAACCGTGCAACAAGCGAACCAAGGACAAC‑AAATTAAACCGCGTTATGAGCCGGCGGCAATCAGTAAAATTATTTTCGCCAACCGTTGGCTGCAACTGCCGATTTATCTCGGGTTGATTATCGTGCAAGGCATTTATGCCTATAAATTTATGAAATCCCTGTGGCATTTAATTGTCAATTTACAGGAAATGGATTCCAACACCGTGa                                                                                                                >  1:395040/1‑251 (MQ=255)                                                                                                                     cAGTTAATTTTTCCGCCTTTTATACTTTTATTTTTTATTGGGGGAAACCGTGCAACAAGCGAACCAAGGACAAC‑AAATTAAACCGCGTTATGAGCCGGCGGCAATCAGTAAAATTATTTTCGCCAACCGTTGGCTGCAACTGCCGATTTATCTCGGGTTGATTATCGTGCAAGGCATTTATGCCTATAAATTTATGAAATCCCTGTGGCATTTAATTGTCAATTTACAGGAAATGGATTCCAACACCGTGa                                                                                                                >  1:711706/1‑251 (MQ=255)                                                                                                                            tttttCCGCCTTTTATACTTTTATTTTTTATTGGGGGAAACCGTGCAACAAGCGAACCAAGGACAAC‑AAATTAAACCGCGTTATGAGCCGGCGGCAATCAGTAAAATTATTTTCGCCAACCGTTGGCTGCAACTGCCGATTTATCTCGGGTTGATTATCGTGCAAGGCATTTATGCCTATAAATTTATGAAATCCCTGTGGCATTTAATTGTCAATTTACAGGAAATGGATTCCAACACCGTGATGTTGGc                                                                                                         <  1:499230/251‑1 (MQ=255)                                                                                                                                    ccTTTTATACTTTTATTTTTTATTGGGGGAAACCGTGCAACAAGCGAACCAAGGACAAC‑AAATTAAACCGCGTTATGAGCCGGCGGCATTCAGTAAAATTATTTTCGCCAACCGTTGGCTGCAACTGCCGATTTATCTCGGGTTGATTATCGTGCAAGGCATTTATGCCTATAAATTTATGAAATCCCTGTGGCATTTAATTg                                                                                                                                                 >  1:558746/1‑203 (MQ=255)                                                                                                                                       tttATACTTTTATTTTTTATTGGGGGAAACCGTGCAACAAGCGAACCAAGGACAAC‑AAATTAAACCGCGTTATGAGCCGGCGGCAATCAGTAAAATTATTTTCGCCAACCGTTGGCTGCAACTGCCGATTTATCTCGGGTTGATTATCGTGCAAGGCATTTATGCCTATAAATTTATGAAATCCCTGTGGCATTTAATTGTCAATTTACAGGAAATGGATTCCAACACCGTGATGTTGGCGGTATTGAATc                                                                                              <  1:431764/251‑1 (MQ=255)                                                                                                                                             cTTTTATTTTTTATTGGGGGAAGCCGTGCAACAAGCGAACCAAGGACAAC‑AAATTAAACCGCGTTATGAGCCGGCGGCAATCAGTAAAATTATTTTCGCCAACCGTTGGCTGCAACTGCCGATTTATCTCGGGTTGATTATCGTGCAAGGCATTTATGCCTATAAATTTATGAAATccc                                                                                                                                                                <  1:19501/179‑1 (MQ=255)                                                                                                                                                ttattttttattGGGGGAAACCGTGCAACAAGCGAACCAAGGACAAC‑AAATTAAACCGCGTTATGAGCCGGCGGCAATCAGTAAAATTATTTTCGCCAACCGTTGGCTGCAACTGCCGATTTATCTCGGGTTGATTATCGTGCAAGGCATTTATGCCTATAAATTTATGAAATCCCTGTGGCATTTAATTGTCAATTTACAGGAAATGGATTCCAACACCGTGATGTTGGCGGTATTGAATCTGATTGAtg                                                                                     <  1:314455/251‑1 (MQ=255)                                                                                                                                                 tattttttattGGGGGAAACCGTGCAACAAGCGAACCAAGGACAAC‑AAATTAAACCGCGTTATGAGCCGGCGGCAATCAGTAAAATTATTTTCGCCAACCGTTGGCTGCAACTGCCGATTTATCTCGGGTTGATTATCGTGCAAGGCATTTATGCCTATAAATTTATGAAATCCCTGTGGCATTTAATTGTCAATTTACAGGAAATGGATTCCAACACCGTGATGTTGGCGGTATTGAATCTGATTGAtgt                                                                                    <  1:124937/251‑1 (MQ=255)                                                                                                                                                  attttttattGGGGGAAACCGTGCAACAAGCGAACCAAGGACAAC‑AAATTAAACCGCGTTATGAGCCGGCGGCAATCAGTAAAATTATTTTCGCCAACCGTTGGCTGCAACTGCCGATTTATCTCGGGTTGATTATCGTGCAAGGCATTTATGCCTATAAATTTATGAAATCCCTGTGGCATTTAATTGTCAATTTACAGGAAATGGATTCCAACACCGTGATGTTGGCGGTATTGAAt                                                                                               >  1:236541/1‑239 (MQ=255)                                                                                                                                                       ttattGGGGGAAACCGTGCAACAAGCGAACCAAGGACAACAAAATTAAACCGCGTTATGAGCCGGCGGCAATCAGTAAAATTATTTTCGCCAACCGTTGGCTGCAACTGCCGATTTATCTCGGGTTGATTATCGTGCAAGGCATTTATGCCTATAAATTTATGAAATCCCTGTGGCATTTAATTGTCAATTTACAGGAAATGGATTCCAACACCGTGATGTTGGCGGTATTGAATCTGATTGATGTTGTCa                                                                               >  1:896095/1‑251 (MQ=255)                                                                                                                                                       ttattGGGGGAAACCGTGCAACAAGCGAACCAAGGACAAC‑AAATTAAACCGCGTTATGAGCCGGCGGCAATCAGTAAAATTATTTTCGCCAACCGTTGGCTGCAACTGCCGATTTATCTCGGGTTGATTATCGTGCAAGGCATTTATGCCTATAAATTTATGAAATCCCTGTGGCATTTAATTGTCAATTTACAGGAAATGGATTCCAacac                                                                                                                     >  1:134562/1‑212 (MQ=255)                                                                                                                                                          ttGGGGGAAACCGTGCAACAAGCGAACCAAGGACAAC‑AAATTAAACCGCGTTATGAGCCGGCGGCAATCAGTAAAATTATTTTCGCCAACCGTTGGCTGCAACTGCCGATTTATCTCGGGTTGATTATCGTGCAAGGCATTTATGCCTATAAATTTATGAAATCCCTGTGGCATTTAATTGTCAATTTACAGGAAATGGATTCCAACACCGTGATGTTGGCGGTATTGAATCTGATTGATGTTGTCATGAt                                                                           <  1:829099/251‑1 (MQ=255)                                                                                                                                                            gggggAAACCGTGCAACAAGCGAACCAAGGACAAC‑AAATTAAACCGCGTTATGAGCCGGCGGCAATCAGTAAAATTATTTTCGCCAACCGTTGGCTGCAACTGCCGATTTATCTCGGGTTGATTATCGTGCAAGGCATTTATGCCTATAAATTTATGAAATCCCTGTGGCATTTAATTGTCAATTTACAGGAAATGGATTCCAacac                                                                                                                     >  1:296518/1‑207 (MQ=255)                                                                                                                                                            gggggAAACCGTGCAACAAGCGAACCAAGGACAAC‑AAATTAAACCGCGTTATGAGCCGGCGGCAATCAGTAAAATTATTTTCGCCAACCGTTGGCTGCAACTGCCGATTTATCTCGGGTTGATTATCGTGCAAGGCATTTATGCCTATAAATTTATGAAATCCCTGTGGCATTTAATTGTCAATTTACAGGAAATGGATTCCAACACCGTGATGt                                                                                                             <  1:871598/215‑1 (MQ=255)                                                                                                                                                            gggggAAACCGTGCAACAAGCGAACCAAGGACAAC‑AAATTAAACCGCGTTATGAGCCGGCGGCAATCAGTAAAATTATTTTCGCCAACCGTTGGCTGCAACTGCCGATTTATCTCGGGTTGATTATCGTGCAAGGCATTTATGCCTATAAATTTATGAAATCCCTGTGGCATTTAATTGTCAATTTACAGGAAATGGATTCCAACACCGTGATGTTGGCGGTATTGAATCTGATTGATGTTGTCATGATCg                                                                         >  1:546730/1‑251 (MQ=255)                                                                                                                                                            gggggAAACCGTGCAACAAGCGAACCAAGGACAAC‑AAATTAAACCGCGTTATGAGCCGGCGGCAATCAGTAAAATTATTTTCGCCAACCGTTGGCTGCAACTGCCGATTTATCTCGGGTTGATTATCGTGCAAGGCATTTATGCCTATAAATTTATGAAATCCCTGTGGCATTTAATTGTCAATTTACAGGAAATGGATTCCAACACCGTGATGTTGGCGGTATTGAATCTGATTGATGTTGTCATGATCg                                                                         >  1:201259/1‑251 (MQ=255)                                                                                                                                                            gaggaaaaCCGTGCAACAAGCGAACCAAGGACGAC‑AAATTAAACCTCGTTATGAGCCGGCGGCATTCAGTAAAATTATTTTCGCCAACCGTTGGCTGCATCTGCCGATTTATCTCGGGTTGATTATCGTGCAAGGCATTTATGCCTATAAATTTATGAAATCCCTGTgg                                                                                                                                                           <  1:64972/164‑1 (MQ=255)                                                                                                                                                              gggAAACCGTGCAACAAGCGAACCAAGGACAAC‑AAATTAAACCGCGTTATGAGCCGGCGGCAATCAGTAAAATTATTTTCGCCAACCGTTGGCTGCAACTGCCGATTTATCTCGGGTTGATTATCGTGCAAGGCATTTATGCCTATAAATTTATGAAATCCCTGTGGCATTTAATTGTCAATTTACAGGAAATGGATTCCAACACCGTGATGTTGGCGGTATTGAATCTGATTGATGTTGTCATGATCGc                                                                        <  1:351901/250‑1 (MQ=255)                                                                                                                                                                    ccGTGCAACAAGCGAACCAAGGACAAC‑AAATTAAACCGCGTTATGAGCCGGCGGCAATCAGTAAAATTATTTTCGCCAACCGTTGGCTGCAACTGCCGATTTATCTCGGGTTGATTATCGTGCAAGGCATTTATGCCTATAAATTTATGAAATCCCTGTGGCATTTAATTGTCAATTTACAGGAAATGGATTCCAACACCg                                                                                                                   >  1:453698/1‑201 (MQ=255)                                                                                                                                                                          aacaaGCGAACCAAGGACAAC‑AAATTAAACCGCGTTATGAGCCGGCGGCAATCAGTAAAATTATTTTCGCCAACCGTTGGCGGCAACTGCCGATTTATCTCGGGTTGATTATCGTGCAAGGCATTTATGCCTATAAATTTATGAAATCCCTGTGGCATTTAATTGTCAATTTACAGGAAATGGATTCCAACACCGTGATGTTGGCGGTATTGAATCTGATTGATGTTGTCATGATCGCCAATTTGCtggtg                                                           <  1:445895/251‑1 (MQ=255)                                                                                                                                                                           acaaGCGAACCAAGGACAAC‑AAATTAAACCGCGTTATGAGCCGGCGGCAATCAGTAAAATTATTTTCGCCAACCGTTGGCTGCAACTGCCGATTTATCTCGGGTTGATTATCGTGCAAGGCATTTATGCCTATAAATTTATGAAATCCCTGTGGCATTTAATTGTCAATTTACAGGAAATGGATTCCAACACCGTGATGTTGGCGGTATTGAATCTGATTGATGTTGTCATGATCGCCAATTTGCtggtga                                                          <  1:674590/251‑1 (MQ=255)                                                                                                                                                                                 gAACCAAGGACAAC‑AAATTAAACCGCGTTATGAGCCGGCGGCAATCAGTAAAATTATTTTCGCCAACCGTTGGCTGCAACTGCCGATTTATCTCGGGTTGATTATCGTGCAAGGCATTTATGCCTATAAATTTATGAAATCCCTGTGGCATTTAATTGTCAATTTACAGGAAATGGATTCCAACACCGTGATGTTGGCGGTATTGAATCTGATTGATGTTGTCATGATCGCCAATTTGCtggtgatggtga                                                    >  1:257605/1‑251 (MQ=255)                                                                                                                                                                                  aaCCAAGGACAAC‑AAATTAAACCGCGTTATGAGCCGGCGGCAATCAGTAAAATTATTTTCGCCAACCGTTGGCTGCAACTGCCGATTTATCTCGGGTTGATTATCGTGCAAGGCATTTATGCCTATAAATTTATGAAATCCCTGTGGCATTTAATTGTCAATTTAc                                                                                                                                        <  1:560936/166‑1 (MQ=255)                                                                                                                                                                                   aCCAAGGACAAC‑AAATTAAACCGCGTTATGAGCCGGCGGCAATCAGTAAAATTATTTTCGCCAACCGTTGGCTGCAACTGCCGATTTATCTCGGGTTGATTATCGTGCAAGGCATTTATGCCTATAAATTTATGAAATCCCTGTGGCATTTAATTGTCAATTTACAgg                                                                                                                                     <  1:60170/168‑1 (MQ=255)                                                                                                                                                                                   aCCAAGGACAAC‑AAATTAAACCGCGTTATGAGCCGGCGGCAATCAGTAAAATTATTTTCGCCAACCGTTGGCTGCAACTGCCGATTTATCTCGGGTTGATTATCGTGCAAGGCATTTATGCCTATAAATTTATGAAATCCCTGTGGCATTTAATTGTCAATTTACAGGAAATGGATTCCAACACCGTGATGTTGGCGGt                                                                                                      <  1:695274/199‑1 (MQ=255)                                                                                                                                                                                   aCCAAGGACAAC‑AAATTAAACCGCGTTATGAGCCGGCGGCAATCAGTAAAATTATTTTCGCCAACCGTTGGCTGCAACTGCCGATTTATCTCGGGTTGATTATCGTGCAAGGCATTTATGCCTATAAATTTATGAAATCCCTGTGGCATTTAATTGTCAATTTACAGGAAATGGATTCCAACACCGTGATGTTGGCGGTATTGAATCTGATTGATGTTGTCATGATCGCCAATTTGCtggtgatgg                                                       >  1:21383/1‑246 (MQ=255)                                                                                                                                                                                     cAAGGACAAC‑AAATTAAACCGCGTTATGAGCCGGCGGCAATCAGTAAAATTATTTTCGCCAACCGTTGGCTGCAACTGCCGATTTATCTCGGGTTGATTATCGTGCAAGGCATTTATGCCTATAAATTTATGAAATCCCTGTGGCATTTAATTGTCAATTTACAGGAAATGGATTCCAACACCGTGATGTTGGCGGTATTGAATCTGATTGATGTTGTCATGATCGCCAATTTGCTGGTGATGGTGATTAt                                                <  1:548919/251‑1 (MQ=255)                                                                                                                                                                                          acaac‑aAATTAAACCGCGTTATGAGCCGGCGGCAATCAGTAAAATTATTTTCGCCAACCGTTGGCTGCAACTGCCGATTTATCTCGGGTTGATTATCGTGCAAGGCATTTATGCCTATAAATTTATGAAATCCCTGTGGCATTTAATTGTCAATTTACAGGAAATGGATTCCAACACCGTGATGTTGGCGGTATTGAATCTGATTGATGTTGTCATGATCGCCAATTTGCTGGTGATGGTGATTATcggcg                                           <  1:819137/251‑1 (MQ=255)                                                                                                                                                                                           caac‑aaATTAAACCGCGTTATGAGCCGGCGGCAATCAGTAAAATTATTTTCGCCAACCGTTGGCTGCAACTGCCGATTTATCTCGGGTTGATTATCGTGCAAGGCATTTATGCCTATAAATTTATGAAATCCCTGTGGCATTTAATTGTCAATTTACAGGAAATGGATTCCAacac                                                                                                                     <  1:799219/176‑1 (MQ=255)                                                                                                                                                                                             ac‑aaATTAAACCGCGTTATGAGCCGGCGGCAATCAGTAAAATTATTTTCGCCAACCGTTGGCTGCAACTGCCGATTTATCTCGGGTTGATTATCGTGCAAGGCATTTATGCCTATAAATTTATGAAATCCCTGTGGCATTTAATTGTCAATTTACAGGAAATGGATTCCAACACCGTGATGTTGGCGGTATTGAATCTGATTGATGTTGTCATGATCGCCAATTTGCTGGTGATGGTGATTATCGGCgggg                                        >  1:785665/1‑250 (MQ=255)                                                                                                                                                                                                aaaTTAAACCGCGTTATGAGCCGGCGGCAATCAGTAAAATTATTTTCGCCAACCGTTGGCTGCAACTGCTGATTTATCTCGGGTTGATTATCGTGCAAGGCATTTATGCCTATAAATTTATGAAATCCCTGTGGCATTTAATTGTCAATTTACAGGAAATGGATTCCAACACCGTGATGTTGGCGGTATTGAATCTGATTGATGTTGTCATGATCGCCAATTTGCTGGTGATGGTGATTATCGGCgggggt                                      >  1:504329/1‑248 (MQ=255)                                                                                                                                                                                                aaaTTAAACCGCGTTATGAGCCGGCGGCAATCAGTAAAATTATTTTCGCCAACCGTTGGCTGCAACTGCCGATTTATCTCGGGTTGATTATCGTGCAAGGCATTTATGCCTATaaa                                                                                                                                                                             >  1:49578/1‑116 (MQ=255)                                                                                                                                                                                                aaaTTAAACCGCGTTATGAGCCGGCGGCAATCAGTAAAATTATTTTCGCCAACCGTTGGCTGCAACTGCCGATTTATCTCGGGTTGATTATCGTGCAAGGCATTTATGCCTATAAATTTATGAAATCCCTGTGGCATTTAATTGTCAATTTACAGGAAATGGa                                                                                                                              >  1:675902/1‑163 (MQ=255)                                                                                                                                                                                                aaaTTAAACCGCGTTATGAGCCGGCGGCAATCAGTAAAATTATTTTCGCCAACCGTTGGCTGCAACTGCCGATTTATCTCGGGTTGATTATCGTGCAAGGCATTTATGCCTATAAATTTATGAAATCCCTGTGGCATTTAATTGTCAATTTACAGGAAATGGATTCCAacac                                                                                                                     >  1:157330/1‑172 (MQ=255)                                                                                                                                                                                                aaaTTAAACCGCGTTATGAGCCGGCGGCAATCAGTAAAATTATTTTCGCCAACCGTTGGCTGCAACTGCCGATTTATCTCGGGTTGATTATCGTGCAAGGCATTTATGCCTATAAATTTATGAAATCCCTGTGGCATTTAATTGTCAATTTACAGGAAATGGATTCCAacac                                                                                                                     >  1:847831/1‑172 (MQ=255)                                                                                                                                                                                                aaaTTAAACCGCGTTATGAGCCGGCGGCAATCAGTAAAATTATTTTCGCCAACCGTTGGCTGCAACTGCCGATTTATCTCGGGTTGATTATCGTGCAAGGCATTTATGCCTATAAATTTATGAAATCCCTGTGGCATTTAATTGTCAATTTACAGGAAATGGATTCCAACACCGTGATGTTGGCGGTATTGAATc                                                                                              >  1:810852/1‑195 (MQ=255)                                                                                                                                                                                                aaaTTAAACCGCGTTATGAGCCGGCGGCAATCAGTAAAATTATTTTCGCCAACCGTTGGCTGCAACTGCCGATTTATCTCGGGTTGATTATCGTGCAAGGCATTTATGCCTATAAATTTATGAAATCCCTGTGGCATTTAATTGTCAATTTACAGGAAATGGATTCCAACACCGTGATGTTGGCGGTATTGAATCtgattg                                                                                        <  1:240015/201‑1 (MQ=255)                                                                                                                                                                                                aaaTTAAACCGCGTTATGAGCCGGCGGCAATCAGTAAAATTATTTTCGCCAACCGTTGGCTGCAACTGCCGATTTATCTCGGGTTGATTATCGTGCAAGGCATTTATGCCTATAAATTTATGAAATCCCTGTGGCATTTAATTGTCAATTTACAGGAAATGGATTCCAACACCGTGATGTTGGCGGTATTGAATCTGATTGATGTTGTCATg                                                                             <  1:130442/212‑1 (MQ=255)                                                                                                                                                                                                aaaTTAAACCGCGTTATGAGCCGGCGGCAATCAGTAAAATTATTTTCGCCAACCGTTGGCTGCAACTGCCGATTTATCTCGGGTTGATTATCGTGCAAGGCATTTATGCCTATAAATTTATGAAATCCCTGTGGCATTTAATTGTCAATTTACAGGAAATGGATTCCAACACCGTGATGTTGGCGGTATTGAATCTGATTGATGTTGTCATGAt                                                                           >  1:477883/1‑214 (MQ=255)                                                                                                                                                                                                aaaTTAAACCGCGTTATGAGCCGGCGGCAATCAGTAAAATTATTTTCGCCAACCGTTGGCTGCAACTGCCGATTTATCTCGGGTTGATTATCGTGCAAGGCATTTATGCCTATAAATTTATGAAATCCCTGTGGCATTTAATTGTCAATTTACAGGAAATGGATTCCAACACCGTGATGTTGGCGGTATTGAATCTGATTGATGTTGTCATGATCGCCAATTTGCTGGTGATGGTGATTATCGGCggggag                                      >  1:859995/1‑248 (MQ=255)                                                                                                                                                                                                 aaTTAAACCGCGTTATGAGCCGGCGGCAATCAGTAAAATTATTTTCGCCAACCGTTGGCTGCAACTGCCGATTTATCTCGGGTTGATTATCGTGCAAGGCATTTATGCCTATAAATTTATGAAATCCCTGTGGCATTTAATTGTCAATTTACAGGAAATGGATTCCAACACCGTGATGTTGGCGGTATTGAATc                                                                                              >  1:551294/1‑194 (MQ=255)                                                                                                                                                                                                           cgTTATGAGCCGGCGGCAATCAGTAAAATTATTTTCGCCAACCGTTGGCTGCAACTGCCGATTTATCTCGGGTTGATTATCGTGCAAGGCATTTATGCCTATAAATTTATGAAATCCCTGTGGCATTTAATTGTCAATTTACAGGAAATGGATTCCAACACCGTGATGTTGGCGGTATTGAATCTGATTGATGTTGTCATGATCGCCAATTTGCTGGTGATGGTGATTATCGGCgggggttaggtttttgt                           >  1:487533/1‑237 (MQ=255)                                                                                                                                                                                                             ttATGAGCCGGCGGCAATCAGTAAAATTATTTTCGCCAACCGTTGGCTGCAACTGCCGATTTATCTCGGGTTGATTATCGTGCAAGGCATTTATGCCTATAAATTTATGAAATCCCTGTGGCATTTAATTGTCAATTTACAGGAAATGGATTCCAACACCGTGATGTTGGCGGTATTGAATCTGATTGAtgt                                                                                    >  1:148591/1‑192 (MQ=255)                                                                                                                                                                                                             ttATGAGCCGGCGGCAATCAGTAAAATTATTTTCGCCAACCGTTGGCTGCAACTGCCGATTTATCTCGGGTTGATTATCGTGCAAGGCATTTATGCCTATAAATTTATGAAATCCCTGTGGCATTTAATTGTCAATTTACAGGAAATGGATTCCAACACCGTGATGTTGGCGGTATTGAATCTGATTGATGTTGTCATGATCGCCAATTTGCTGGTGATGGTGATTATCGGCgggggtgtgatttttgttt                         >  1:210822/1‑235 (MQ=255)                                                                                                                                                                                                               aTGAGCCGGCGGCAATCAGTAAAATTATTTTCGCCAACCGTTGGCTGCAACTGCCGATTTATCTCGGGTTGATTATCGTGCAAGGCATTTATGCCTATAAATTTATGAAATCCCTGTGGCATTTAATTGTCAATTTACAGGAAATGGATTCCAACACCGTGATGTTGGCGGTATTGAATCTGATTGATGTTGTCATGa                                                                            <  1:368533/198‑1 (MQ=255)                                                                                                                                                                                                                                  tAAAATTATTTTCGCCAACCGTTGGCTGCAACTGCCGATTTATCTCGGGTTGATTATCGTGCAAGGCATTTATGCCTATAAATTTATGAAATCCCTGTGGCATTTAATTGTCAATTTACAGGAAATGGATTCCAACACCGTGATGTTGGCGGTATTGAATCTGATTGATGTTGTCATGATCGCCAATTTGCTGGTGATGGTGATTATCGGCGGGTATGAGATTTTCGTCTCCAAGCTGCGCACGCGTGGGc    <  1:149298/251‑1 (MQ=255)                                                                                                                                                                                                                                    aaaTTATTTTCGCCAACCGTTGGCTGCAACTGCCGATTTATCTCGGGTTGATTATCGTGCAAGGCATTTATGCCTATAAATTTATGAAATCCCTGTGGCATTTAATTGTCAATTTACAGGAAATGGATTCCAACACCGTGATGTTGGCGGTATTGAATCTGATTGATGTTGTCATGATCGCCAATTTGCTGGTGATGGTGATTATCGGCGGGTATGAGATTTTCGTCTCCAAGCTGCGCACGCGTGGGCAt  <  1:357334/251‑1 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                            CGGAGCGGTTGTTGGTGACAAAAGAAAATTTAGAAGAATAAAAGTGCGGTGGATTTTTAATGTGTTTTTACAATTTCCCCATGATGTTAAAAAAACGTTGAATTTTAGGCGTAAAACAGTTAATTTTTCCGCCTTTTATACTTTTATTTTTTATTGGGGGAAACCGTGCAACAAGCGAACCAAGGACAAC‑AAATTAAACCGCGTTATGAGCCGGCGGCAATCAGTAAAATTATTTTCGCCAGCCGTTGGCTGCAACTGCCGATTTATCTCGGGTTGATTATCGTGCAAGGCATTTATGCCTATAAATTTATGAAATCCCTGTGGCATTTAATTGTCAATTTACAGGAAATGGATTCCAACACCGTGATGTTGGCGGTATTGAATCTGATTGATGTTGTCATGATCGCCAATTTGCTGGTGATGGTGATTATCGGCGGGTATGAGATTTTCGTCTCCAAGCTGCGCACGCGTGGGCAT  >  1/401206‑401682

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑