Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	2,092,599	A→G	R159R (CGT→CGC)	unknown ←	Pyruvate formate‑lyase (EC 2.3.1.54)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	2,092,599	0	A	G	100.0%	199.1 / NA	70	R159R (CGT→CGC)	unknown	Pyruvate formate‑lyase (EC 2.3.1.54)
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (45/25);  total (45/25)

TACCCAATGCGCGATGTTGTTCGGCAATTTCTTCACGTAATTGAATGGTGGCTTGAATGTCTTCACCACGTTCTAATTTAGGTTGTAAGGAAGCGAATTGTTTTTTCTTATCCGCCATCAAGAAATCGACACCATAAACCGCTAAACGACGATAGTCACCGATAATACGACCACGACCGTAAGCATCCGGCAAACCGGTTAACACGCCGGATTTACGGCAACGAAGAATGTCCGGTGTATAAACGTCGAAAACACCTTGGTTGTGGGTTTTACGATATTCGGTAAAGATATGTTCTACTTCAGGATCTAATTGACGACCATAAACTTCGCAAGATCCTTTCACCATTTTGATACCACCGAACGGCATAATTGCACGTTTTAACGGTGCATCGGTTTGTAAACCGACGATTTTTTCCAATTCTTTATTGATATAACCCGGTTTGTGAGAAGTGATTGTAGA  >  1/2092379‑2092838                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                tACCCAATGCGCGATGTTGTTCGGCAATTTCTTCACGTAATTGAATGGTGGCTTGAATGTCTTCACCACGTTCTAATTTAGGTTGTAAGGAAGCGAATTGTTTTTTCTTATCCGCCATCAAGAAATCGACACCATAAACCGCTAAACGACGATAGTCACCGATAATACGACCACGACCGTAAGCATCCGGCAAACCGGTTAACACGCCGGATTTACGGCAGCGAAGAATGTCCGGTGTATAAACGTCGaaa                                                                                                                                                                                                                   <  1:589893/251‑1 (MQ=255)  aCCCAATGCGCGATGTTGTTCGGCAATTTCTTCACGTAATTGAATGGTGGCTTGAATGTCTTCACCACGTTCTAATTTAGGTTGTAAGGAAGCGAATTGTTTTTTCTTATCCGCCATCAAGAAATCGACACCATAAACCGCTAAACGACGATAGTCACCGATAATACGACCACGACCGTAAGCATCCGGCAAACCGGTTAACACGCCGGATTTACGGCAGCGAAGAATGTCCGGTGTATAAACGTCGaaaa                                                                                                                                                                                                                  >  1:167275/1‑251 (MQ=255)              aTGTTGTTCGGCAATTTCTTCACGTAATTGAATGGTGGCTTGAATGTCTTCACCACGTTCTAATTTAGGTTGTAAGGAAGCGAATTGTTTTTTCTTATCCGCCATCAAGAAATCGACACCATAAACCGCTAAACGACGATAGTCACCGATAATACGACCACGACCGTAAGCATCCGGCAAACCGGTTAACACGCCGGATTTACGGCAGCGAAGAATGTCCGGTGTATAAACGTCGAAAACACCTTGGTtgt                                                                                                                                                                                                      >  1:652628/1‑251 (MQ=255)              aTGTTGTTCGGCAATTTCTTCACGTAATTGAATGGTGGCTTGAATGTCTTCACCACGTTCTAATTTAGGTTGTAAGGAAGCGAATTGTTTTTTCTTATCCGCCATCAAGAAATCGACACCATAAACCGCTAAACGACGATAGTCACCGATAATACGACCACGACCGTAAGCATCCGGCAAACCGGTTAACACGCCGGATTTACGGCAGCGAAGAATGTCCGGTGTATAAACGTCGAAAACACCTTGGTtgg                                                                                                                                                                                                      >  1:49715/1‑250 (MQ=255)                 ttgttCGGCAATTTCTTCACGTAATTGAATGGTGGCTTGAATGTCTTCACCACGTTCTAATTTAGGTTGTAAGGAAGCGAATTGTTTTTTCTTATCCGCCATCAAGAAATCGACACCATAAACCGCTAAACGACGATAGTCACCGATAATACGACCACGACCGTAAGCATCCGGCAAACCGGTTAACACGCCGGATTTACGGCAGCGAAGAATGTCCGGTGTATAAACGTCGAAAACACCTTGGTTGTggg                                                                                                                                                                                                   >  1:61593/1‑251 (MQ=255)                  tgttCGGCAATTTCTTCACGTAATTGAATGGTGGCTTGAATGTCTTCACCACGTTCTAATTTAGGTTGTAAGGAAGCGAATTGTTTTTTCTTATCCGCCATCAAGAAATCGACACCATAAACCGCTAAACGACGATAGTCACCGATAATACGACCACGACCGTAAGCATCCGGCAAACCGGTTAACACGCCGGATTTACGGCAGCGAAGAATGTCCGGTGTATAAACGTCGAAAACACCTTGGTTGTGGGt                                                                                                                                                                                                  <  1:751988/251‑1 (MQ=255)                                      tAATTGAATGGTGGCTTGAATGTCTTCACCACGTTCTAATTTAGGTTGTAAGGAAGCGAATTGTTTTTTCTTATCCGCCATCAAGAAATCGACACCATAAACCGCTAAACGACGATAGTCACCGATAATACGACCACGACCGTAAGCATCCGGCAAACCGGTTAACACGCCGGATTTACGGCAGCGAAGAATGTc                                                                                                                                                                                                                                      <  1:537282/195‑1 (MQ=255)                                      tAATTGAATGGTGGCTTGAATGTCTTCACCACGTTCTAATTTAGGTTGTAAGGAAGCGAATTGTTTTTTCTTATCCGCCATCAAGAAATCGACACCATAAACCGCTAAACGACGATAGTCACCGATAATACGACCACGACCGTAAGCATCCGGCAAACCGGTTAACACGCCGGATTTACGGCAGCGAAGAATGTCCGGTGTATAAACGTCGAAAACACCTTGGTTGTGGGTTTTACGa                                                                                                                                                                                           >  1:790699/1‑238 (MQ=255)                                      tAATTGAATGGTGGCTTGAATGTCTTCACCACGTTCTAATTTAGGTTGTAAGGAAGCGAATTGTTTTTTCTTATCCGCCATCAAGAAATCGACACCATAAACCGCTAAACGACGATAGTCACCGATAATACGACCACGACCGTAAGCATCCGGCAAACCGGTTAACACGCCGGATTTACGGCAGCGAAGAATGTCCGGTGTATAAACGTCGAAAACACCTTGGTTGTGGGTTTTACGa                                                                                                                                                                                           >  1:724379/1‑238 (MQ=255)                                      tAATTGAATGGTGGCTTGAATGTCTTCACCACGTTCTAATTTAGGTTGTAAGGAAGCGAATTGTTTTTTCTTATCCGCCATCAAGAAATCGACACCATAAACCGCTAAACGACGATAGTCACCGATAATACGACCACGACCGTAAGCATCCGGCAAACCGGTTAACACGCCGGATTTACGGCAGCGAAGAATGTCCGGTGTATAAACGTCGAAAACACCTTGGTTGTGGGTTTTACGATATTCGGTAAAGa                                                                                                                                                                              >  1:48401/1‑251 (MQ=255)                                      tAATTGAATGGTGGCTTGAATGTCTTCACCACGTTCTAATTTAGGTTGTAAGGAAGCGAATTGTTTTTTCTTATCCGCCATCAAGAAATCGACACCATAAACCGCTAAACGACGATAGTCACCGATAATACGACCACGACCGTAAGCATCCGGCAAACCGGTTAACACGCCGGATTTACGGCAGCGAAGAATGTCCGGTGTATAAACGTCGAAAACACCTTGGTTGTGGGTTTTACGATATTCGGTAAAGa                                                                                                                                                                              >  1:666871/1‑251 (MQ=255)                                      tAATTGAATGGTGGCTTGAATGTCTTCACCACGTTCTAATTTAGGTTGTAAGGAAGCGAATTGTTTTTTCTTATCCGCCATCAAGAAATCGACACCATAAACCGCTAAACGACGATAGTCACCGATAATACGACCACGACCGTAAGCATCCGGCAAACCGGTTAACACGCCGGATTTACGGCAGCGAAGAATGACCGGTGTATAAACGTCGAAAACACCTTGGTTGTGGGTTTTACGATATTCGGTAAAGa                                                                                                                                                                              >  1:229926/1‑251 (MQ=255)                                      tAATTGAATGGTGGCTTGAATGTCTTCACCACGTTCTAA‑TTAGGTTGTAAGGAAGCGAATTGTTTTTTCTTATCCGCCATCAAGAAATCGACACCATAAACCGCTAAACGACGATAGTCACCGATAATACGACCACGACCGTAAGCATCCGGCAAACCGGTTAACACGCCGGATTTACGGCAGCGAAGAATGTCCGGTGTATAAACGTCGAAAACACCTTGGTTGTGGGTTTTACGATATTCGGTAAAGat                                                                                                                                                                             >  1:880327/1‑251 (MQ=255)                                        aTTGAATGGTGGCTTGAATGTCTTCACCACGTTCTAATTTAGGTTGTAAGGAAGCGAATTGTTTTTTCTTATCCGCCATCAAGAAATCGACACCATAAACCGCTAAACGACGATAGTCACCGATAATACGACCACGACCGTAAGCATCCGGCAAACCGGTTAACACGCCGGATTTACGGCAGCGAAGAATGTCCGGTGTATAAACGTCGAAAACACCt                                                                                                                                                                                                             <  1:40787/218‑1 (MQ=255)                                        aTTGAATGGTGGCTTGAATGTCTTCACCACGTTCTAATTTAGGTTGTAAGGAAGCGAATTGTTTTTTCTTATCCGCCATCAAGAAATCGACACCATAAACCGCTAAACGACGATAGTCACCGATAATACGACCACGACCGTAAGCATCCGGCAAACCGGTTAACACGCCGGATTTACGGCAGCGAAGAATGTCCGGTGTATAAACGTCGAAAACACCTTGGTTGTGGGTTTTAc                                                                                                                                                                                             >  1:386112/1‑234 (MQ=255)                                        aTTGAATGGTGGCTTGAATGTCTTCACCACGTTCTAATTTAGGTTGTAAGGAAGCGAATTGTTTTTTCTTATCCGCCATCAAGAAATCGACACCATAAACCGCTAAACGACGATAGTCACCGATAATACGACCACGACCGTAAGCATCCGGCAAACCGGTTAACACGCCGGATTTACGGCAGCGAAGAATGTCCGGTGTATAAACGTCGAAAACACCTTGGTTGTGGGTTTTACGATATTCGGTAAAGata                                                                                                                                                                            >  1:606025/1‑251 (MQ=255)                                                 tggCTTGAATGTCTTCACCACGTTCTAATTTAGGTTGTAAGGAAGCGAATTGTTTTTTCTTATCCGCCATCAAGAAATCGACACCATAAACCGCTAAACGACGATAGTCACCGATAATACGACCACGACCGTAAGCATCCGGCAAACCGGTTAACACGCCGGATTTACGGCAGCGAAGAATGTCCGGTGTATAAACGTCGAAAACACCTTGGTTGTGGGTTTTACGATATTCGGTAAAGATATGTTCTAc                                                                                                                                                                    <  1:324453/250‑1 (MQ=255)                                                  ggCTTGAATGTCTTCACCACGTTCTAATTTAGGTTGTAAGGAAGCGAATTGTTTTTTCTTATCCGCCATCAAGAAATCGACACCATAAACCGCTAAACGACGATAGTCACCGATAATACGACCACGACCGTAAGCATCCGGCAAACCGGTTAACACGCCGGATTTACGGCAGCGAAGAATGTCCGGTGTATAAACg                                                                                                                                                                                                                         >  1:366426/1‑196 (MQ=255)                                                  ggCTTGAATGTCTTCACCACGTTCTAATTTAGGTTGTAAGGAAGCGAATTGTTTTTTCTTATCCGCCATCAAGAAATCGACACCATAAACCGCTAAACGACGATAGTCACCGATAATACGACCACGACCGTAAGCATCCGGCAAACCGGTTAACACGCCGGATTTACGGCAGCGAAGAATGTCCGGTGTATAAACGTCGAAAACACCTTGGTTGTGGGTTTTACGATATTCGGTAAAGATATGTTCTACtt                                                                                                                                                                  >  1:318825/1‑251 (MQ=255)                                                   gCTTGAATGTCTTCACCACGTTCTAATTTAGGTTGTAAGGAAGCGAATTGTTTTTTCTTATCCGCCATCAAGAAATCGACACCATAAACCGCTAAACGACGATAGTCACCGATAATACGACCACGACCGTAAGCATCCGGCAAACCGGTTAACACGCCGGATTTACGGCAGCGAAGAATGTCCGGTGTATAAACGTCGAAAACACCTTGGTTGTGGGTTTTACGATATTCGGTAAAGATATGTTCTACtt                                                                                                                                                                  <  1:395849/250‑1 (MQ=255)                                                    cTTGAATGTCTTCACCACGTTCTAATTTAGGTTGTAAGGAAGCGAATTGTTTTTTCTTATCCGCCATCAAGAAATCGACACCATAAACCGCTAAACGACGATAGTCACCGATAATACGACCACGACCGTAAGCATCCGGCAAACCGGTTAACACGCCGGATTTACGGCAGCGAAGAATGTCCGGTGTATAAACGTCGAAAACACCTTGGTTGTGGGTTTTACGATATTCGGTAAAGATATGTTCTACTTc                                                                                                                                                                 <  1:90304/250‑1 (MQ=255)                                                        aaTGTCTTCACCACGTTCTAATTTAGGTTGTAAGGAAGCGAATTGTTTTTTCTTATCCGCCATCAAGAAATCGACACCATAAACCGCTAAACGACGATAGTCACCGATAATACGACCACGACCGTAAGCATCCGGCAAACCGGTTAACACGCCGGATTTACGGCAGCGAAGAATGTCCGGTGTATAAACGTCGAAAACACCTTGGTTGTGGGTTTTACGATATTCGGTAAAGATATGTTCTACTTCAGGAt                                                                                                                                                            <  1:577665/251‑1 (MQ=255)                                                          tGTCTTCACCACGTTCTAATTTAGGTTGTAAGGAAGCGAATTGTTTTTTCTTATCCGCCATCAAGAAATCGACACCATAAACCGCTAAACGACGATAGTCACCGATAATACGACCACGACCGTAAGCATCCGGCAAACCGGTTAACACGCCGGATTTACGGCAGCGAAGAATGTCCGGTGTATAAACGTCGAAAACACCTTGGTTGTGGGTTTTACGATATTCGGTAAAGATATGTTCTACTTCAGGAt                                                                                                                                                            >  1:876736/1‑249 (MQ=255)                                                           gTCTTCACCACGTTCTAATTTAGGTTGTAAGGAAGCGAATTGTTTTTTCTTATCCGCCATCAAGAAATCGACACCATAAACCGCTAAACGACGATAGTCACCGATAATACGACCACGACCGTAAGCATCCGGCAAACCGGTTAACACGCCGGATTTACGGCAGCGAAGAATGTCCGGTGTATAAACGTCGAAAACACCTTGGTtg                                                                                                                                                                                                       >  1:380102/1‑205 (MQ=255)                                                           gTCTTCACCACGTTCTAATTTAGGTTGTAAGGAAGCGAATTGTTTTTTCTTATCCGCCATCAAGAAATCGACACCATAAACCGCTAAACGACGATAGTCACCGATAATACGACCACGACCGTAAGCATCCGGCAAACCGGTTAACACGCCGGATTTACGGCAGCGAAGAATGTCCGGTGTATAAACGTCGAAAACACCTTGGTTGTGGGTTTTACGATATTCGGTAAAGATATGtt                                                                                                                                                                        >  1:79805/1‑236 (MQ=255)                                                            tCTTCACCACGTTCTAATTTAGGTTGTAAGGAAGCGAATTGTTTTTTCTTATCCGCCATCAAGAAATCGACACCATAAACCGCTAAACGACGATAGTCACCGATAATACGACCACGACCGTAAGCATCCGGCAAACCGGTTAACACGCCGGATTTACGGCAGCGAAGAATGTCCGGTGTATAAACGTCGAAAACACCTTGGTTGTGGGTTTTACGATATTCGGTAAAGata                                                                                                                                                                            >  1:569283/1‑231 (MQ=255)                                                             cTTCACCACGTTCTAATTTAGGTTGTAAGGAAGCGAATTGTTTTTTCTTATCCGCCATCAAGAAATCGACACCATAAACCGCTAAACGACGATAGTCACCGATAATACGACCACGACCGTAAGCATCCGGCAAACCGGTTAACACGCCGGATTTACGGCAGCgaagaa                                                                                                                                                                                                                                          >  1:799519/1‑168 (MQ=255)                                                             cTTCACCACGTTCTAATTTAGGTTGTAAGGAAGCGAATTGTTTTTTCTTATCCGCCATCAAGAAATCGACACCATAAACCGCTAAACGACGATAGTCACCGATAATACGACCACGACCGTAAGCATCCGGCAAACCGGTTAACACGCCGGATTTACGGCAGCGAAGAATGTCCGGTGTATAAACGTCGAAAACACCTTGGTTGTGGGTTTTACGATATTCGGTAAAGATATGTTCTAc                                                                                                                                                                    >  1:99414/1‑238 (MQ=255)                                                                caccacGTTCTAATTTAGGTTGTAAGGAAGCGAATTGTTTTTTCTTATCCGCCATCAAGAAATCGACACCATAAACCGCTAAACGACGATAGTCACCGATAATACGACCACGACCGTAAGCATCCGGCAAACCGGTTAACACGCCGGATTTACGGCAGCGAAGAATGTCCGGTGTATAAACGTCGAAAACACCTTGGTTGTGGGTTTTACGATATTCGGTAAAGATATGTTCTACTTCAGGATCTAATTga                                                                                                                                                    >  1:116688/1‑251 (MQ=255)                                                                      gTTCTAATTTAGGTTGTAAGGAAGCGAATTGTTTTTTCTTATCCGCCATCAAGAAATCGACACCATAAACCGCTAAACGACGATAGTCACCGATAATACGACCACGACCGTAAGCATCCGGCAAACCGGTTAACACGCCGGATTTACGGCAGCGAAGAATGTCCGGTGTATAAACGTCGAAAACACCTTGGTTGTGGGTTTTACGATATTCGGTAAAGATATGTTCTACTTCAGGATCTAAt                                                                                                                                                       >  1:727310/1‑242 (MQ=255)                                                                       ttCTAATTTAGGTTGTAAGGAAGCGAATTGTTTTTTCTTATCCGCCATCAAGAAATCGACACCATAAACCGCTAAACGACGATAGTCACCGATAATACGACCACGACCGTAAGCATCCGGCAAACCGGTTAACACGCCGGATTTACGGCAGCGAAGAATGTCCGGTGTATAAACGTCGAAAACACCTTGGTTGTGGGTTTTACGa                                                                                                                                                                                           >  1:179845/1‑205 (MQ=255)                                                                       ttCTAATTTAGGTTGTAAGGAAGCGAATTGTTTTTTCTTATCCGCCATCAAGAAATCGACACCATAAACCGCTAAACGACGATAGTCACCGATAATACGACCACGACCGTAAGCATCCGGCAAACCGGTTAACACGCCGGATTTACGGCAGCGAAGAATGTCCGGTGTATAAACGTCGAAAACACCTTGGTTGTGGGTTTTACGATATTCGGTAAAGATATGTTCTACTTCAGGATCTAATTGACGACCAt                                                                                                                                             >  1:622950/1‑251 (MQ=255)                                                                           aaTTTAGGTTGTAAGGAAGCGAATTGTTTTTTCTTATCCGCCATCAAGAAATCGACACCATAAACCGCTAAACGACGATAGTCACCGATAATACGACCACGACCGTAAGCATCCGGCAAACCGGTTAACACGCCGGATTTACGGCAGCGAAGAATGTCCGGTGTATAAACGTCGAAAACACCTTGGTTGTGGGTTTTACGATATTCGGTAAAGATATGTTCTACTTCAGGATCTAATTGACGACCATAAAc                                                                                                                                         <  1:594091/251‑1 (MQ=255)                                                                                 ggTTGTAAGGAAGCGAATTGTTTTTTCTTATCCGCCATCAAGAAATCGACACCATAAACCGCTAAACGACGATAGTCACCGATAATACGACCACGACCGTAAGCATCCGGCAAACCGGTTAACACGCCGGATTTACGGCAGCGAAGAATGTCCGGTGTATAAACGTCGAAAACACCTTGGTTGTGGGTTTTACGATATTCGGTAAAGATATGTTCTACTTc                                                                                                                                                                 >  1:755843/1‑221 (MQ=255)                                                                                  gTTGTAAGGAAGCGAATTGTTTTTTCTTATCCGCCATCAAGAAATCGACACCATAAACCGCTAAACGACGATAGTCACCGATAATACGACCACGACCGTAAGCATCCGGCAAACCGGTTAACACGCCGGATTTACGGCAGCGAAGAATGTCCGGTGTATAAACGTCGAAAACACCTTGGTTGTGGGTTTTACGATATTCGGTAAAGATATGTTCTACTTCAGGATCTAATTGACGACCATAAACTTCGCa                                                                                                                                   <  1:791661/250‑1 (MQ=255)                                                                                   ttGTAAGGAAGCGAATTGTTTTTTCTTATCCGCCATCAAGAAATCGACACCATAAACCGCTAAACGACGATAGTCACCGATAATACGACCACGACCGTAAGCATCCGGCAAACCGGTTAACACGCCGGATTTACGGCAGCGAAGAATGTCCGGTGTATAAACGTCGAAAACACCTTGGTTGTGGGTTTTACGATATTCGGTAAAGATATGTTCTACTTCAg                                                                                                                                                               <  1:830554/221‑1 (MQ=255)                                                                                      aaaGGAAGCGAATTGTTTTTTCTTATCCGCCATCAAGAAATCGACACCATAAACCGCTAAACGACGATAGTCACCGATAATACGACCACGACCGTAAGCATCCGGCAAACCGGTTAACACGCCGGATTTACGGCAGCGAAGAATGTCCGGTGTATAAACGTCGAAAACACCTTGGTTGTGGGTTTTACGATATTCGGTAAAGATATGTTCTACTTCAGGATCTAATTGACGACCATAAACTTCGCAAGATc                                                                                                                              <  1:425264/250‑1 (MQ=255)                                                                                               gAATTGTTTTTTCTTATCCGCCATCAAGAAATCGACACCATAAACCGCTAAACGACGATAGTCACCGATAATACGACCACGACCGTAAGCATCCGGCAAACCGGTTAACACGCCGGATTTACGGCAGCGAAGAATGTCCGGTGTATAAACGTCGAAAACACCTTGGTTGTGGGTTTTACGATATTCGGTAAAGATATGTTCTACTTCAGGATCTAATTGACGACCATAAACTTCGCAAGATCCTTTCACCa                                                                                                                     >  1:575122/1‑251 (MQ=255)                                                                                                   tGTTTTTTCTTATCCGCCATCAAGAAATCGACACCATAAACCGCTAAACGACGATAGTCACCGATAATACGACCACGACCGGAAGCATCCGGCAAACCGGTTAACACGCCGGATTTACGGCAGCGAAGAATGTCCGGTGTATAAACGTCGAAAACACCTTGGTTGTGGGTTTTACGATATTCGGTAAAGATATGTTCTACTTCAGGATCTAATTGACGACCATAAACTTCGCAAGATCCTTTCACCAtttt                                                                                                                 <  1:445915/251‑1 (MQ=255)                                                                                                    gTTTTTTCTTATCCGCCATCAAGAAATCGACACCATAAACCGCTAAACGACGATAGTCACCGATAATACGACCACGACCGTAAGCATCCGGCAAACCGGTTAACACGCCGGATTTACGGCAGCGAAGAATGTCCGGTGTATAAACGTCGAAAACACCTTGGTTGTGGGTTTTACGATATTCGGTAAAGATATGTTCTACTTCAGGATCTAAt                                                                                                                                                       >  1:369605/1‑212 (MQ=255)                                                                                                    gTTTTTTCTTATCCGCCATCAAGAAATCGACACCATAAACCGCTAAACGACGATAGTCACCGATAATACGACCACGACCGTAAGCATCCGGCAAACCGGTTAACACGCCGGATTTACGGCAGCGAAGAATGTCCGGTGTATAAACGTCGAAAACACCTTGGTTGTGGGTTTTACGATATTCGGTAAAGATATGTTCTACTTCAGGATCTAATTgac                                                                                                                                                   >  1:562073/1‑216 (MQ=255)                                                                                                    gTTTTTTCTTATCCGCCATCAAGAAATCGACACCATAAACCGCTAAACGACGATAGTCACCGATAATACGACCACGACCGTAAGCATCCGGCAAACCGGTTAACACGCCGGATTTACGGCAGCGAAGAATGTCCGGTGTATAAACGTCGAAAACACCTTGGTTGTGGGTTTTACGATATTCGGTAAAGATATGTTCTACTTCAGGATCTAATTGACGACCATAAACTTCGCAAGATCCTTTCACCATTTTg                                                                                                                >  1:395763/1‑251 (MQ=255)                                                                                                         ttCTTATCCGCCATCAAGAAATCGACACCATAAACCGCTAAACGACGATAGTCACCGATAATACGACCACGACCGTAAGCATCCGGCAAACCGGTTAACACGCCGGATTTACGGCAGCGAAGAATGTCCGGTGTATAAACGTCGAAAACACCTTGGTTGTGGGTTTTACGATATTCGGTAAAGATATGTTCTACTTCAGGATCTAATTGACGACCATAAACTTCGCAAGATCCTTTCACCATTTTGATacc                                                                                                           <  1:898918/251‑1 (MQ=255)                                                                                                           cTTATCCGCCATCAAGAAATCGACACCATAAACCGCTAAACGACGATAGTCACCGATAATACGACCACGACCGTAAGCATCCGGCAAACCGGTTAACACGCCGGATTTACGGCAGCGAAGAATGTCCGGTGTATAAACGTCGAAAACACCTTGGTTGTGGGTTTTACGATATTCGGTAAAGATATGTTCTACTTCAGGATCTAATTGACGACCATAAACTTCGCAAGATCCTTTCACCATTTTGATaccac                                                                                                         <  1:113834/251‑1 (MQ=255)                                                                                                                  gCCATCAAGAAATCGACACCATAAACCGCTAAACGACGATAGTCACCGATAATACGACCACGACCGTAAGCATCCGGCAAACCGGTTAACACGCCGGATTTACGGCAGCGAAGAATGTCCGGTGTATAAACGTCGAAAACACCTTGGTTGTGGGTTTTACGATATTCGGTAAAGATATGTTCTACTTCAGGATCTAATTGACGACCATAAACTTCGCAAGATCCTTTCACCATTTTGATa                                                                                                             >  1:707301/1‑240 (MQ=255)                                                                                                                                    ccATAAACCGCTAAACGACGATAGTCACCGATAATACGACCACGACCGTAAGCATCCGGCAAACCGGTTAACACGCCGGATTTACGGCAGCGAAGAATGTCCGGTGTATAAACGTCGAAAACACCTTGGTTGTGGGTTTTACGATATTCGGTAAAGATATGTTCTACtt                                                                                                                                                                  >  1:777786/1‑169 (MQ=255)                                                                                                                                    ccATAAACCGCTAAACGACGATAGTCACCGATAATACGACCACGACCGTAAGCATCCGGCAAACCGGTTAACACGCCGGATTTACGGCAGCGAAGAATGTCCGGTGTATAAACGTCGAAAACACCTTGGTTGTGGGTTTTACGATATTCGGTAAAGATATGTTCTACTTCAGGATCTAATTGACGACCATAAACTTCGCAAGATCCTTTCACCATTTTGATACCACCGAACGGCATAATTGCACGTTTTaa                                                                                >  1:549267/1‑251 (MQ=255)                                                                                                                                    ccATAAACCGCTAAACGACGATAGTCACCGATAATACGACCACGACCGTAAGCATCCGGCAAACCGGTTAACACGCCGGATTTACGGCAGCGAAGAATGTCCGGTGAATAAACGTCGAAAACACCTTGGTTGTGGGTTTTACGATATTCGGTAAAGATATGTTCTACTTCAGGATCTAATTGACGACCATAAACTTCGCAAGATCCTTTCACCATTTTGATACCACCGAACGGCATAATTGCACGTTTTaa                                                                                >  1:806980/1‑251 (MQ=255)                                                                                                                                    ccATAAACCGCTAAACGACGATAGTCACCGATAATACGACCACGACCGTAAGCATCCGGCAAACCGGTTAACACGCCGGATTTACGGCAGCGAAGAATGCCCGGTGTATAAACGTCGAAAACACCTTGGTTGTGGGTTTTACGATATTCGGTAAAGATATGTTCTACTTCAGGATCTAATTGACGACCATAAACTTCGCAAGATCCTTTCACCATTTTGATACCACCGAACGGCATAATTGCACGTTTTaa                                                                                >  1:883246/1‑251 (MQ=255)                                                                                                                                       tAAACCGCTAAACGACGATAGTCACCGATAATACGACCACGACCGTAAGCATCCGGCAAACCGGTTAACACGCCGGATTTACGGCAGCGAAGAATGTCCGGTGTATAAACGTCGAAAACACCTTGGTTGTGGGTTTTACGATATTCGGTAAAGATATGTTCTACTTCAGGATCTAATTGACGACCATAAACTTCGCAAGATCCTTTCACCATTTTGATACCACCGAACGGCATAATTGCACGTTTTAACgg                                                                             <  1:344367/251‑1 (MQ=255)                                                                                                                                             gCTAAACGACGATAGTCACCGATAATACGACCACGACCGTAAGCATCCGGCAAACCGGTTAACACGCCGGATTTACGGCAGCGAAGAATGTCCGGTGTATAAACGTCGAAAACACCTTGGTTGTGGGTTTTACGATATTCGGTAAAGATATGTTCTACTTCAGGATCTAATTGACGACCATAAACTTCGCAAGATCCTTTc                                                                                                                         >  1:237031/1‑201 (MQ=255)                                                                                                                                                   cgacgaTAGTCACCGATAATACGACCACGACCGTAAGCATCCGGCAAACCGGTTAACACGCCGGATTTACGGCAGCGAAGAATGTCCGGTGTATAAACGTCGAAAACACCTTGGTTGTGGGTTTTACGATATTCGGTAAAGATATGTTc                                                                                                                                                                       <  1:577163/149‑1 (MQ=255)                                                                                                                                                   cgacgaTAGTCACCGATAATACGACCACGACCGTAAGCATCCGGCAAACCGGTTAACACGCCGGATTTACGGCAGCGAAGAATGTCCGGTGTATAAACGTCGAAAACACCTTGGTTGTGGGTTTTACGATATTCGGTAAAGATATGTTCTACTTCAGGAt                                                                                                                                                            >  1:23448/1‑160 (MQ=255)                                                                                                                                                   cgacgaTAGTCACCGATAATACGACCACGACCGTAAGCATCCGGCAAACCGGTTAACACGCCGGATTTACGGCAGCGAAGAATGTCCGGTGTATAAACGTCGAAAACACCTTGGTTGTGGGTTTTACGATATTCGGTAAAGATATGTTCTACTTCAGGATCTAATTGACGACCATAAACTTCGCAAGATCCTTTCACCATTTTGATACCACCGAACGGCATAATTGCACGTTTTAACGGTGCATCGGtt                                                                   >  1:1117/1‑249 (MQ=255)                                                                                                                                                                 gATAATACGACCACGACCGTAAGCATCCGGCAAACCGGTTAACACGCCGGATTTACGGCAGCGAAGAATGTCCGGTGTATAAACGTCGAAAACACCTTGGTTGTGGGTTTTACGATATTCGGTAAAGATATGTTCTACTTCAGGATCTAATTGACGACCATAAACTTCGCAAGATCCTTTCACCATTTTGATACCACCGAACGGCATAATTGCACGTTTTAACGGTGCATCgg                                                                     >  1:606386/1‑233 (MQ=255)                                                                                                                                                                     ataCGACCACGACCGTAAGCATCCGGCAAACCGGTTAACACGCCGGATTTACGGCAGCGAAGAATGTCTGGTGTATAAACGTCGAAAACACCTTGGTTGTGGGTTTTACGATATTCGGTAAAGATATGTTCTACTTCAGGATCTAATTGACGACCATAAACTTCGCAAGATCCTTTCACCATTTTGATACCACCGAACGGCATAATTGCACGTTTTAACGGTGCATCGGTTTGTAAACCGACGAtttttt                                                >  1:367268/1‑250 (MQ=255)                                                                                                                                                                         gaccacgaccGTAAGCATCCGGCAAACCGGTTAAAACGCCGGATTTACGGCAGCGAAGAATGTCCGGTGTATAAACGTCGAAAACACCTTGGTTGTGGGTTTTACGATATTCGGTAAAGATATGTTCTACTTCAGGATCTAATTGACGACCATAAACTTCGCAAGATCCTTTCACCATTTTGATACCACCGAACGGCATAATTGCACGTTTTAACGGTGCATCGGTTTGTAAACCGACGATTTTTTCCAAt                                           >  1:569048/1‑251 (MQ=255)                                                                                                                                                                          accacgaccGTAAGCATCCGGCAAACCGGTTAACACGCCGGATTTACGGCAGCGAAGAATGTCCGGTGTATAAACGTCGAAAACACCTTGGTTGTGGGTTTTACGATATTCGGTAAAGATATGTTCTACTTCAGGATCTAATTGACGACCATAAACTTCGCAAGATCCTTTCACCATTTTGATACCACCGAACGGCATAATTGCACGTTTTAACGGTGCAt                                                                        >  1:631161/1‑221 (MQ=255)                                                                                                                                                                                accGTAAGCATCCGGCAAACCGGTTAACACGCCGGATTTACGGCAGCGAAGAATGTCCGGTGTATAAACGTCGAAAACACCTTGGTTGTGGGTTTTACGATATTCGGTAAAGATATGTTCTACTTCAGGATCTAATTGACGACCATAAACTTCGCAAGATCCTTTCACCATTTTGATACCACCGAACGGCATAATTGCACGTTTTAACGGTGCATCGGTTTGTAAACCGACGATTTTTTCCAATTCTTTAt                                    <  1:634855/251‑1 (MQ=255)                                                                                                                                                                                   gTAAGCATCCGGCAAACCGGTTAACACGCCGGATTTACGGCAGCGAAGAATGTCCGGTGTATAAACGTCGAAAACACCTTGGTTGTGGGTTTTACGATATTCGGTAAAGATATGTTCTACTTCAGGATCTAATTGACGACCATAAACTTCGCAAGAt                                                                                                                               >  1:715266/1‑157 (MQ=255)                                                                                                                                                                                         aTCCGGCAAACCGGTTAACACGCCGGATTTACGGCAGCGAAGAATGTCCGGTGTATAAACGTCGAAAACACCTTGGTTGTGGGTTTTACGATATTCGGTAAAGATATGTTCTACTTCAGGATCTAATTGACGACCATAAACTTCGCAAGATCCTTTCACCATTTTGATACCACCGAACGGCATAATTGCACGTTTTAACGGTGCATCGGTTTGTAAACCGACGATTTTTTCCAATTCTTTATTGATATAAc                           <  1:425455/251‑1 (MQ=255)                                                                                                                                                                                                     ggTTAACACGCCGGATTTACGGCAGCGAAGAATGTCCGGTGTATAAACGTCGAAAACACCTTGGTTGTGGGTTTTACGATATTCGGTAAAGATATGTTCTACTTCAGGATCTAATTGACGACCATAAACTTCGCAAGATCCTTTCACCATTTTGATACCACCGAACGGCATAATTGCACGTTTTAACGGTGCATCGGTTTGTAAACCGACGATTTTTTCCAATTCTTTATTGATATAACCCGGTTTGTgag               >  1:672838/1‑251 (MQ=255)                                                                                                                                                                                                        tAACACGCCGGATTTACGGCAGCGAAGAATGTCCGGTGTATAAACGTCGAAAACACCTTGGTTGTGGGTTTTACGATATTCGGTAAAGATATGTTCTACTTCAGGATCTAATTGACGACCATAAACTTCGCAAGATCCTTTCACCATTTTGATACCACCGAACGGCATAATTGCACGTTTTAACGGTGCATCGGTTTGTAAACCGACGATTTTTTCCAATTCTTTATTGATATAACCCGGTTTGTGAGAAg            <  1:201176/251‑1 (MQ=255)                                                                                                                                                                                                               ccGGATTTACGGCAGCGAAGAATGTCCGGTGTATAAACGTCGAAAACACCTTGGTTGTGGGTTTTACGATATTCGGTAAAGATATGTTCTACTTCAGGATCTAATTGACGACCATAAACTTCGCAAGATCCTTTCACCATTTTGATACCACCGAACGGCATAATTGCACGTTTTAACGGTGCATCGGTTTGTAAACCGACGATTTTTTCCAATTCTTTATTGATATAACCCGGTTTGTGAGAAGTGATTg      <  1:705795/250‑1 (MQ=255)                                                                                                                                                                                                               ccGGATTTACGGCAGCGAAGAATGTCCGGTGTATAAACGTCGAAAACACCTTGGTTGTGGGTTTTACGATATTCGGTAAAGATATGTTCTACTTCAGGATCTAATTGACGACCATAAACTTCGCAAGATCCTTTCACCATTTTGATACCACCGAACGGCATAATTGCACGTTTTAACGGTGCATCGGTTTGTAAACCGACGATTTTTTCCAATTCTTTATTGATATAACCCGGTTTGTGAGAAGTGATTg      <  1:304037/250‑1 (MQ=255)                                                                                                                                                                                                                 ggATTTACGGCAGCGAAGAATGTCCGGTGTATAAACGTCGAAAACACCTTGGTTGTGGGTTTTACGATATTCGGTAAAGATATGTTCTACTTCAGGATCTAATTGACGACCATAAACTTCGCAAGATCCTTTCACCATTTTGATACCACCGAACGGCATAATTGCACGTTTTAACGGTGCATCGGTTTGTAAACCGACGATTTTTTCCAATTCTTTATTGATATAACCCGGTTTGTGAGAAGTGATTGTAg   >  1:396779/1‑251 (MQ=255)                                                                                                                                                                                                                  gATTTACGGCAGCGAAGAATGTCCGGTGTATAAACGTCGAAAACACCTTGGTTGTGGGTTTTACGATATTCGGTAAAGATATGTTCTACTTCAGGATCTAATTGACGACCATAAACTTCGCAAGATCCTTTCACCATTTTGATACCACCGAAc                                                                                                    <  1:298940/153‑1 (MQ=255)                                                                                                                                                                                                                  gATTTACGGCAGCGAAGAATGTCCGGTGTATAAACGTCGAAAACACCTTGGTTGTGGGTTTTACGATATTCGGTAAAGATATGTTCTACTTCAGGATCTAATTGACGACCATAAACTTCGCAAGATCCTTTCACCATTTTGATACCACCGAACGGCATAATTGCACGTTTTAACGGTGCATCGGTTTGTAAACCGACGATTTTTTCCAATTCTTTATTGATATAAccc                         <  1:599627/228‑1 (MQ=255)                                                                                                                                                                                                                   aTTTACGGCAGCGAAGAATGTCCGGTGTATAAACGTCGAAAACACCTTGGTTGTGGGTTTTACGATATTCGGTAAAGATATGTTCTACTTCAGGATCTAATTGACGACCATAAACTTCGCAAGATCCTTTCACCATTTTGATACCACCGAACGGCATAATTGCACGTTTTAACGGTGCATCGGTTTGTAAACCGACGATTTTTTCCAATTCTTTATTg                                  >  1:119160/1‑218 (MQ=255)                                                                                                                                                                                                                   aTTTACGGCAGCGAAGAATGTCCGGTGTATAAACGTCGAAAACACCTTGGTTGTGGGTTTTACGATATTCGGTAAAGATATGTTCTACTTCAGGATCTAATTGACGACCATAAACTTCGCAAGATCCTTTCACCATTTTGATACCACCGAACGGCATAATTGCACGTTTTAACGGTGCATCGGTTTGTAAACCGACGATTTTTTCCAATTCTTTATTGATATAACCCGGTTTGTGAGAAGTGATTGTAGa  <  1:659450/250‑1 (MQ=255)                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                TACCCAATGCGCGATGTTGTTCGGCAATTTCTTCACGTAATTGAATGGTGGCTTGAATGTCTTCACCACGTTCTAATTTAGGTTGTAAGGAAGCGAATTGTTTTTTCTTATCCGCCATCAAGAAATCGACACCATAAACCGCTAAACGACGATAGTCACCGATAATACGACCACGACCGTAAGCATCCGGCAAACCGGTTAACACGCCGGATTTACGGCAACGAAGAATGTCCGGTGTATAAACGTCGAAAACACCTTGGTTGTGGGTTTTACGATATTCGGTAAAGATATGTTCTACTTCAGGATCTAATTGACGACCATAAACTTCGCAAGATCCTTTCACCATTTTGATACCACCGAACGGCATAATTGCACGTTTTAACGGTGCATCGGTTTGTAAACCGACGATTTTTTCCAATTCTTTATTGATATAACCCGGTTTGTGAGAAGTGATTGTAGA  >  1/2092379‑2092838

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑