Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	2,079,321	T→C	N101D (AAC→GAC)	unknown ←	Cell envelope opacity‑associated protein A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	2,079,321	0	T	C	100.0%	205.4 / NA	71	N101D (AAC→GAC)	unknown	Cell envelope opacity‑associated protein A
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (31/40);  total (31/40)

AGATAAAAATCAGCACCAACACCACGCCGAATAGCGCGACGAAAATGCGACGATGTTTTTGCGGGAGAATACTTAAAATCGCCCAATTTTCAGGATTTTTAAAACCGGCTTTCGGTTTTTCTTTCAATTCGGCAGGTTCCGGTTGTGTTGCTTCAACGGCTTCTTCCGCTACGGTAACGAGAGGTTCGTCCGGGGCTTCTGACGGGGTTTCCGTCGCCGTGTCGGCGGCAATCTGCGGTTCGTTGCTTTCTGTGTTTTCTATTGAAAATCTCTCGGTCGGGTTTTCGCTTGTCATGCTTTTCTCTCCAGTCGCACCGGCACCACCGAATACGGGTTCTCTGCGAACGGCAAATTGATTTTCCATATTCTTTTGTTCTTTCTTCGTAAGGAGATCTTTGCCTTTTCCAAGCAACTTCTCAAACAAAGACGGTTCCGGTTTAATCACTTTTTTCGGGGTGATCGGTTCCATTTGACTAAAGCCCAAATCCAAT  >  1/2079078‑2079568                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                        aGATAAAAATCAGCACCAACACCACGCCGAATAGCGCGACGAAAATGCGACGATGTTTTTGCGGGAGAATACTTAAAATCGCCCAATTTTCAGGATTTTTAAAACCGGCTTTCGGTTTTTCTTTCAATTCGGCAGGTTCCGGTTGTGTTGCTTCAACGGCTTCTTCCGCTACGGTAACGAGAGGTTCGTCCGGGGCTTCTGACGGGGTTTCCGTCGCCGTGTCGGCGGCAATCTGCGGTTCGTCGCTTTc                                                                                                                                                                                                                                                   >  1:543806/1‑250 (MQ=255)  gATAAAAATCAGCACCAACACCACGCCGAATAGCGCGACGAAAATGCGACGATGTTTTTGCGGGAGAATACTTAAAATCGCCCAATTTTCAGGATTTTTAAAACCGGCTTTCGGTTTTTCTTTCAATTCGGCAGGTTCCGGTTGTGTTGCTTCAACGGCTTCTTCCGCTACGGTAACGAGAGGTTCGTCCGGGGCTTCTGACGGGGTTTCCGTCGCCGTGTCGGCGGCAATCTGCGGTTCGTCGCttt                                                                                                                                                                                                                                                    >  1:661769/1‑248 (MQ=255)             gCACCAACACCACGCCGAATAGCGCGACGAAAATGCGACGATGTTTTTGCGGGAGAATACTTAAAATCGCCCAATTTTCAGGATTTTTAAAACCGGCTTTCGGTTTTTCTTTCAATTCGGCAGGTTCCGGTTGTGTTGCTTCAACGGCTTCTTCCGCTACGGTAACGAGAGGTTCGTCCGGGGCTTCTGACGGGGTTTCCGTCGCCGTGTCGGCGGCAATCTGCGGTTCGTCGCTTTCTGTGTTTTCt                                                                                                                                                                                                                                         <  1:189312/248‑1 (MQ=255)             gCACCAACACCACGCCGAATAGCGCGACGAAAATGCGACGATGTTTTTGCGGGAGAATACTTAAAATCGCCCAATTTTCAGGATTTTTAAAACCGGCTTTCGGTTTTTCTTTCAATTCGGCAGGTTCCGGTTGTGTTGCTTCAACGGCTTCTTCCGCTACGGTAACGAGAGGTTCGTCCGGGGCTTCTGACGGGGTTTCCGTCGCCGTGTCGGCGGCAATCTGCGGTTCGTCGCTTTCTGTGTTTTCTAtt                                                                                                                                                                                                                                      >  1:142922/1‑251 (MQ=255)                   acacCACGCCGAATAGCGCGACGAAAATGCGACGATGTTTTTGCGGGAGAATACTTAAAATCGCCCAATTTTCAGGATTTTTAAAACCGGCTTTCGGTTTTTCTTTCAATTCGGCAGGTTCCGGTTGTGTTGCTTCAACGGCTTCTTCCGCTACGGTAACGAGAGGTTCGTCCGGGGCTTCTGACGGGGTTTCCGTCGCCGTGTCGGCGGCAATCTGCGGTTCGTCGCTTTCTGTGTTTTCTATTGAAAAt                                                                                                                                                                                                                                <  1:522525/251‑1 (MQ=255)                        acGCCGAATAGCGCGACGAAAATGCGACGATGTTTTTGCGGGAGAATACTTAAAATCGCCCAATTTTCAGGATTTTTAAAACCGGCTTTCGGTTTTTCTTTCAATTCGGCAGGTTCCGGTTGTGTTGCTTCAACGGCTTCTTCCGCTACGGTAACGAGAGGTTCGTCCGGGGCTTCTGACGGGGTTTCCGTCGCCGTGTCGGCGGCAATCTGCGGTTCGTCGCTTTCTGTGTTTTCTATTGAAAAtctct                                                                                                                                                                                                                            >  1:847460/1‑250 (MQ=255)                             gAATAGCGCGACGAAAATGCGACGATGTTTTTGCGGGAGAATACTTAAAATCGCCCAATTTTCAGGATTTTTAAAACCGGCTTTCGGTTTTTCTTTCAATTCGGCAGGTTCCGGTTGTGTTGCTTCAACGGCTTCTTCCGCTACGGTAACGAGAGGTTCGTCCGGGGCTTCTGACGGGGTTTCCGTCGCCGTGTCGGCGGCAATCTGCGGTTCGTCGCTTTCTGTGTTTTCTATTGAAAATCTCtcggtcg                                                                                                                                                                                                                      >  1:364478/1‑251 (MQ=255)                                       acgaAAATGCGACGATGTTTTTGCGGGAGAATACTTAAAATCGCCCAATTTTCAGGATTTTTAAAACCGGCTTTCGGTTTTTCTTTCAATTCGGCAGGTTCCGGTTGTGTTGCTTCAACGGCTTCTTCCGCTACGGTAACGAGAGGTTCGTCCGGGGCTTCTGACGGGGTTTCCGTCGCCGTGTCGGCGGCAATCTGCGGTTCGTCGCTTTCTGTGTTTTCTATTGAAAATCTCTCGGTCGGGTTTTCGc                                                                                                                                                                                                             <  1:516561/250‑1 (MQ=255)                                                          tttGCGGGAGAATACTTAAAATCGCCCAATTTTCAGGATTTTTAAAACCGGCTTTCGGTTTTTCTTTCAATTCGGCAGGTTCCGGTTGTGTTGCTTCAACGGCTTCTTCCGCTACGGTAACGAGAGGTTCGTCCGGGGCTTCTGACGGGGTTTCCGTCGCCGTGTCGGCGGCAATCTGCGGTTCGTCGCTTTCTGTGTTTTCTATTGAAAATCTCTCGGTCGGGTTTTCGCTTGTCATGCTTTTCTCTCCa                                                                                                                                                                                         >  1:710284/1‑251 (MQ=255)                                                           ttGCGGGAGAATACTTAAAATCGCCCAATTTTCAGGATTTTTAAAACCGGCTTTCGGTTTTTCTTTCAATTCGGCAGGTTCCGGTTGTGTTGCTTCAACGGCTTCTTCCGCTACGGTAACGAGAGGTTCGTCCGGGGCTTCTGACGGGGTTTCCGTCGCCGTGTCGGCGGCAATCTGCGGTTCGTCGCTTTCTGTGTTTTCTATTGAAAATCTCTCGGTCGGGTTTTCGCTTGTCATGCTTTTCTCTCCAg                                                                                                                                                                                        <  1:818840/251‑1 (MQ=255)                                                                     aTACTTAAAATCGCCCAATTTTCAGGATTTTTAAAACCGGCTTTCGGTTTTTCTTTCAATTCGGCAGGTTCCGGTTGTGTTGCTTCAACGGCTTCTTCCGCTACGGTAACGAGAGGTTCGTCCGGGGCTTCTGACGGGGTTTCCGTCGCCGTGTCGGCGGCAATCTGCGGTTCGTCGCTTTCTGTGTTTTCTATTGAAAATCTCTCGGTCGGGTTTTCGCTTGTCATGCTTTTCTCTCCAGTCGCACCgg                                                                                                                                                                               >  1:402026/1‑250 (MQ=255)                                                                     aTACTTAAAATCGCCCAATTTTCAGGATTTTTAAAACCGGCTTTCGGTTTTTCTTTCAATTCGGCAGGTTCCGGTTGTGTTGCTTCAACGGCTTCTTCCGCTACGGTAACGAGAGGTTCGTCCGGGGCTTCTGACGGGGTTTCCGTCGCCGTGTCGGCGGCAATCTGCGGTTCGTCGCTTTCTGTGTTTTCTATTGAAAATCTCTCGGTCGGGTTTTCGCTTGTCATGCTTTTCTCTCCAGTCGCACCgg                                                                                                                                                                               >  1:201023/1‑250 (MQ=255)                                                                     aTACTTAAAATCGCCCAATTTTCAGGATTTTTAAAACCGGCTTTCGGTTTTTCTTTCAATTCGGCAGGTTCCGGTTGTGTTGCTTCAACGGCTTCTTCCGCTACGGTAACGAGAGGTTCGTCCGGGGCTTCTGACGGGGTTTCCGTCGCCGTGTCGGCGGCAATCTGCGGTTCGTCGCTTTCTGTGTTTTCTATTGAAAATCTCTCGGTCGGGTTTTCGCTTGTCATGCTTTTCTCTCCAGTCGCACCGGc                                                                                                                                                                              <  1:476525/251‑1 (MQ=255)                                                                      tACTTAAAATCGCCCAATTTTCAGGATTTTTAAAACCGGCTTTCGGTTTTTCTTTCAATTCGGCAGGTTCCGGTTGTGTTGCTTCAACGGCTTCTTCCGCTACGGTAACGAGAGGTTCGTCCGGGGCTTCTGACGGGGTTTCCGTCGCCGTGTCGGCGGCAATCTGCGGTTCGTCGCTTTCTGTGTTTTCTATTGAAAATCTCTCGGTCGGGTTTTCGCTTGTCATGCTTTTCTCTCCAGTCGCACCGGca                                                                                                                                                                             >  1:114677/1‑251 (MQ=255)                                                                       aCTTAAAATCGCCCAATTTTCAGGATTTTTAAAACCGGCTTTCGGTTTTTCTTTCAATTCGGCAGGTTCCGGTTGTGTTGCTTCAACGGCTTCTTCCGCTACGGTAACGAGAGGTTCGTCCGGGGCTTCTGACGGGGTTTCCGTCGCCGTGTCGGCGGCAATCTGCGGTTCGTCGCTTTCTGTGTTTTCTATTGAAAATCTCTCGGTCGGGTTTTCGCTTGTCATGCTTTTCTCTCCAGTCGCACCGGcac                                                                                                                                                                            <  1:272470/251‑1 (MQ=255)                                                                       aCTTAAAATCGCCCAATTTTCAGGATTTTTAAAACCGGCTTTCGGTTTTTCTTTCAATTCGGCAGGTTCCGGTTGTGTTGCTTCAACGGCTTCTTCCGCTACGGTAACGAGAGGTTCGTCCGGGGCTTCTGACGGGGTTTCCGTCGCCGTGTCGGCGGCAATCTGCGGTTCGTCGCTTTCTGTGTTTTCTATTGAAAATCTCTCGGTCGGGTTTTCGCTTGTCATGCTTTTCTCTCCAGTCGCACCGGcac                                                                                                                                                                            <  1:291919/251‑1 (MQ=255)                                                                          tAAAATCGCCCAATTTTCAGGATTTTTAAAACCGGCTTTCGGTTTTTCTTTCAATTCGGCAGGTTCCGGTTGTGTTGCTTCAACGGCTTCTTCCGCTACGGTAACGAGAGGTTCGTCCGGGGCTTCTGACGGGGTTTCCGTCGCCGTGTCGGCGGCAATCTGCGGTTCGTCGCTTTCTGTGTTTTCTATTGAAAATCTCTCGGTCGGGTTTTCGCTTGTCATGCTTTTCTCTCCAGTCGCACCGGcaccac                                                                                                                                                                         <  1:254695/251‑1 (MQ=255)                                                                           aaaaTCGCCCAATTTTCAGGATTTTTAAAACCGGCTTGCGGTTTTTCTTTCAATTCGGCAGGTTCCGGTTGTGTTGCTTCAACGGCTTCTTCCGCTACGGTAACGAGAGGTTCGTCCGGGGCTTCTGACGGGGTTTCCGTCGCCGTGTCGGCGGCAATCTGCGGTTCGTCGCTTTCTGTGTTTTCTATTGAAAATCTCTCGGTCGGGTTTTCGCTTGTCATGCTTTTCTCTCCAGTCGCACCGGCaccacc                                                                                                                                                                        <  1:828081/251‑1 (MQ=255)                                                                                 gCCCAATTTTCAGGATTTTTAAAACCGGCTTTCGGTTTTTCTTTCAATTCGGCAGGTTCCGGTTGTGTTGCTTCAACGGCTTCTTCCGCTACGGTAACGAGAGGTTCGTCCGGGGCTTCTGACGGGGTTTCCGTCGCCGTGTCGGCGGCAATCTGCGGTTCGTCGCTTTCTGTGTTTTCTATTGAAAATCTCTCGGTCGGGTTTTCGCTTGTCATGCTTTTCTCTCCAGTCGCACCGGCACCACCGAATa                                                                                                                                                                   >  1:116654/1‑250 (MQ=255)                                                                                  cccAATTTTCAGGATTTTTAAAACCGGCTTTCGGTTTTTCTTTCAATTCGGCAGGTTCCGGTTGTGTTGCTTCAACGGCTTCTTCCGCTACGGTAACGAGAGGTTCGTCCGGGGCTTCTGACGGGGTTTCCGTCGCCGTGTCGGCGGCAATCTGCGGTTCGTCGCTTTCTGTGTTTTCTATTGAAAATCTCTCGGTCGGGTTTTCGCTTGTCATGCTTTTCTCTCCAGTCGCACCGGCACCACCGAATAc                                                                                                                                                                  <  1:642568/250‑1 (MQ=255)                                                                                    cAATTTTCAGGATTTTTAAAACCGGCTTTCGGTTTTTCTTTCAATTCGGCAGGTTCCGGTTGTGTTGCTTCAACGGCTTCTTCCGCTACGGTAACGAGAGGTTCGTCCGGGGCTTCTGACGGGGTTTCCGTCGCCGTGTCGGCGGCAATCTGCGGTTCGTCGCTTTCTGTGTTTTCTATTGAAAATCTCTCGGTCGGGTTTTCGCTTGTCATGCTTTTCTCTCCAGTCGCACCGGCACCACCGAATACggg                                                                                                                                                               <  1:78446/251‑1 (MQ=255)                                                                                      aTTTTCAGGATTTTTAAAACCGGCTTTCGGTTTTTCTTTCAATTCGGCAGGTTCCGGTTGTGTTGCTTCAACGGCTTCTTCCGCTACGGTAACGAGAGGTTCGTCCGGGGCTTCTGACGGGGTTTCCGTCGCCGTGTCGGCGGCAATCTGCGGTTCGTCGCTTTCTGTGTTTTCTATTGAAAATCTCTCGGTCGGGTTTTCGCTTGTCATGCt                                                                                                                                                                                                   >  1:826761/1‑213 (MQ=255)                                                                                      aTTTTCAGGATTTTTAAAACCGGCTTTCGGTTTTTCTTTCAATTCGGCAGGTTCCGGTTGTGTTGCTTCAACGGCTTCTTCCGCTACGGTAACGAGAGGTTCGTCCGGGGCTTCTGACGGGGTTTCCGTCGCCGTGTCGGCGGCAATCTGCGGTTCGTCGCTTTCTGTGTTTTCTATTGAAAATCTCTCGGTCGGGTTTTCGCTTGTCATGCTTTTCTCTCCAGTCGCACCGGCACCACCGAATACGGGtt                                                                                                                                                             >  1:626220/1‑251 (MQ=255)                                                                                      aTTTTCAGGATTTTT‑AAACCGGCTTTCGGTTTTTCTTTCAATTCGGCAGGTTCCGGTTGTGTTGCTTCAACGGCTTCTTCCGCTACGGTAACGAGAGGTTCGTCCGGGGCTTCTGACGGGGTTTCCGTCGCCGTGTCGGCGGCAATCTGCGGTTCGTCGCTTTCTGTGTTTTCTATTGAAAATCTCTCGGTCGGGTTTTCGCTTGTCATGCTTTTCTCTCCAGTCGCACCGGcaccac                                                                                                                                                                         <  1:754955/238‑1 (MQ=255)                                                                                       ttttCAGGATTTTTAAAACCGGCTTTCGGTTTTTCTTTCAATTCGGCAGGTTCCGGTTGTGTTGCTTCAACGGCTTCTTCCGCTACGGTAACGAGAGGTTCGTCCGGGGCTTCTGACGGGGTTTCCGTCGCCGTGTCGGCGGCAATCTGCGGTTCGTCGCTTTCTGTGTTTTCTATTGAAAATCTCTCGGTCGGGTTTTCGCTTGTCATGCTTTTCTCTCCAGTCGCACCGGCACCACCGAATACGGGTtc                                                                                                                                                            <  1:680863/251‑1 (MQ=255)                                                                                              gATTTTTAAAACCGGCTTTCGGTTTTTCTTTCAATTCGGCAGGTTCCGGTTGTGTTGCTTCAACGGCTTCTTCCGCTACGGTAACGAGAGGTTCGTCCGGGGCTTCTGACGGGGTTTCCGTCGCCGTGTCGGCGGCAATCTGCGGTTCGTCGCTTTCTGTGTTTTCTATTGAAAATCTCTCGGTCGGGTTTTCGCTTGTCATGCTTTTCTCTCCAGTCGCACCGGCACCACCGAATACGGGTTCTCTg                                                                                                                                                        <  1:164494/248‑1 (MQ=255)                                                                                                          cGGCTTTCGGTTTTTCTTTCAATTCGGCAGGTTCCGGTTGTGTTGCTTCAACGGCTTCTTCCGCTACGGTAACGAGAGGTTCGTCCGGGGCTTCTGACGGGGTTTCCGTCGCCGTGTCGGCGGCAATCTGCGGTTCGTCGCTTTCTGTGTTTTCTATTGAAAATCTCTCGGTCGGGTTTTCGCTTGTCATGCTTTTCTCTCCAGTCGCACCGGCACCACCGAATACGGGTTCTCTGCGAACGGCAAATTGa                                                                                                                                         <  1:283855/251‑1 (MQ=255)                                                                                                           ggCTTTCGGTTTTTCTTTCAATTCGGCAGGTTCCGGTTGTGTTGCTTCAACGGCTTCTTCCGCTACGGTAACGAGAGGTTCGTCCGGGGCTTCTGACGGGGTTTCCGTCGCCGTGTCGGCGGCAATCTGCGGTTCGTCGCTTTCTGTGTTTTCTATTGAAAATCTCTCGGTCGGGTTTTCGCTTGTCATGCTTTTCTCTCCAGTCGCACCGGCACCACCGAATACGGGTtc                                                                                                                                                            <  1:329124/231‑1 (MQ=255)                                                                                                                      tttctttcAATTCGGCAGGTTCCGGTTGTGTTGCTTCAACGGCTTCTTCCGCTACGGTAACGAGAGGTTCGTCCGGGGCTTCTGACGGGGTTTCCGTCGCCGTGTCGGCGGCAATCTGCGGTTCGTCGCTTTCTGTGTTTTCTATTGAAAATCTCTCGGTCGGGTTTTCGCTTGTCATGCTTTTCTCTCCAGTCGCACCGGCACCACCGAATACGGGTTCTCTGCGAACGGCAAATTGATTTTCCATATTc                                                                                                                             <  1:658790/251‑1 (MQ=255)                                                                                                                         ctttcAATTCGGCAGGTTCCGGTTGTGTTGCTTCAACGGCTTCTTCCGCTACGGTAACGAGAGGTTCGTCCGGGGCTTCTGACGGGGTTTCCGTCGCCGTGTCGGCGGCAATCTGCGGTTCGTCGCTTTCTGTGTTTTCTATTGAAAATCTCTCGGTCGGGTTTTCGCTTGTCATGCTTTTCTCTCCAGTCGCACCGGCACCACCGAATACGGGTTCTCTGCGAACGGCAAATTGATTTTCCATATTCttt                                                                                                                          <  1:725313/251‑1 (MQ=255)                                                                                                                             cAATTCGGCAGGTTCCGGTTGTGTTGCTTCAACGGCTTCTTCCGCTACGGTAACGAGAGGTTCGTCCGGGGCTTCTGACGGGGTTTCCGTCGCCGTGTCGGCGGCAATCTGCGGTTCGTCGCTTTCTGTGTTTTCTATTGAAAATCTCTCGGTCGGGTTTTCGCTTGTCATGCTTTTCTCTCCAGTCGCACCGGCACCACCGAATACGGGTTCTCTGCGAACGGCAAATTGATTTTCCATATTc                                                                                                                             <  1:658921/244‑1 (MQ=255)                                                                                                                              aaTTCGGCAGGTTCCGGTTGTGTTGCTTCAACGGCTTCTTCCGCTACGGTAACGAGAGGTTCGTCCGGGGCTTCTGACGGGGTTTCCGTCGCCGTGTCGGCGGCAATCTGCGGTTCGTCGCTTTCTGTGTTTTCTATTGAAAATCTCTCGGTCGGGTTTTCGCTTGTCATGCTTTTCTCTCCAGTCGCACCGGCACCACCGAATACGGGTTCTCTGCGAACGGCAAATTGATTTTCCATATTCTTTTGttc                                                                                                                     <  1:160466/251‑1 (MQ=255)                                                                                                                               aTTCGGCAGGTTCCGGTTGTGTTGCTTCAACGGCTTCTTCCGCTACGGTAACGAGAGGTTCGTCCGGGGCTTCTGACGGGGTTTCCGTCGCCGTGTCGGCGGCAATCTGCGGTTCGTCGCTTTCTGTGTTTTCTATTGAAAATCTCTCGGTCGGGTTTTCGCTTGTCATGCTTTTCTCTCCAGTCGCACCGGCACCACCGAATACGGGTTCTCTGCGAACGGCAAATTGATTTTCCATATTCTTTTGtt                                                                                                                      >  1:120399/1‑249 (MQ=255)                                                                                                                               aTTCGGCAGGTTCCGGTTGTGTTGCTTCAACGGCTTCTTCCGCTACGGTAACGAGAGGTTCGTCCGGGGCTTCTGACGGGGTTTCCGTCGCCGTGTCGGCGGCAATCTGCGGTTCGTCGCTTTCTGTGTTTTCTATTGAAAATCTCTCGGTCGGGTTTTCGCTTGTCATGCTTTTCTCTCCAGTCGCACCGGCACCACCGAATACGGGTTCTCTGCGAACGGCAAATTGATTTTCCATATTCTTTTGtt                                                                                                                      >  1:718408/1‑249 (MQ=255)                                                                                                                                       ggTTCCGGTTGTGTTGCTTCAACGGCTTCTTCCGCTACGGTAACGAGAGGTTCGTCCGGGGCTTCTGACGGGGTTTCCGTCGCCGTGTCGGCGGCAATCTGCGGTTCGTCGCTTTCTGTGTTTTCTATTGAAAATCTCTCGGTCGGGTTTTCGCTTGTCATGCTTTTCTCTCCAGTCGCACCGGCACCACCGAATAc                                                                                                                                                                  >  1:599611/1‑197 (MQ=255)                                                                                                                                         ttCCGGTTGTGTTGCTTCAACGGCTTCTTCCGCTACGGTAACGAGAGGTTCGTCCGGGGCTTCTGACGGGGTTTCCGTCGCCGTGTCGGCGGCAATCTGCGGTTCGTCGCTTTCTGTGTTTTCTATTGAAAATCTCTCGGTCGGGTTTTCGCTTGTCATGCTTTTCTCTCCAGTCGCACCGGCACCACCGAATACGGGTtc                                                                                                                                                            <  1:203853/201‑1 (MQ=255)                                                                                                                                             ggTTGTGTTGCTTCAACGGCTTCTTCCGCTACGGTAACGAGAGGTTCGTCCGGGGCTTCTGACGGGGTTTCCGTCGCCGTGTCGGCGGCAATCTGCGGTTCGTCGCTTTCTGTGTTTTCTATTGAAAATCTCTCGGTCGGGTTTTCGCTTGTCATGCTTTTCTCTCCAGTCGCACCGGCACCACCGAATACGGGTTCTCTGCGAAc                                                                                                                                                   <  1:808122/206‑1 (MQ=255)                                                                                                                                             ggTTGTGTTGCTTCAACGGCTTCTTCCGCTACGGTAACGAGAGGTTCGTCCGGGGCTTCTGACGGGGTTTCCGTCGCCGTGTCGGCGGCAATCTGCGGTTCGTCGCTTTCTGTGTTTTCTATTGAAAATCTCTCGGTCGGGTTTTCGCTTGTCATGCTTTTCTCTCCAGTCGCACCGGCACCACCGAATACGGGTTCTCTGCGAACGGCAAATTGATTTTCCATATTCTTTTGTTCTTTCTTCGCAAGgag                                                                                                      >  1:893246/1‑251 (MQ=255)                                                                                                                                              gTTGTGTTGCTTCAACGGCTTCTTCCGCTACGGTAACGAGAGGTTCGTCCGGGGCTTCTGACGGGGTTTCCGTCGCCGTGTCGGCGGCAATCTGCGGTTCGTCGCTTTCTGTGTTTTCTATTGAAAATCTCTCGGTCGGGTTTTCGCTTGTCATGCTTTTCTCTCCAGTCGCACCGGCACCACCGAATACGGGTTCTCTGCGAACGGCAAATTGATTTTCCATATTCTTTTGTTCTTTCTTCGCAAGgaga                                                                                                     >  1:593731/1‑251 (MQ=255)                                                                                                                                                 gtgtTGCTTCAACGGCTTCTTCCGCTACGGTAACGAGAGGTTCGTCCGGGGCTTCTGACGGGGTTTCCGTCGCCGTGTCGGCGGCAATCTGCGGTTCGTCGCTTTCTGTGTTTTCTATTGAAAATCTCTCGGTCGGGTTTTCGCTTGTCATGCTTTTCTCTCCAGTCGCACCGGCACCACCGAATACGGGTTCTCTGCGAACGGCAAATTGATTTTCCATATTCTTTTGTTCTTTCTTCGCAAGGAGAt                                                                                                    >  1:863795/1‑249 (MQ=255)                                                                                                                                                                 ttcttcCGCTACGGTAACGAGAGGTTCGTCCGGGGCTTCTGACGGGGTTTCCGTCGCCGTGTCGGCGGCAATCTGCGGTTCGTCGCTTTCTGTGTTTTCTATTGAAAATCTCTCGGTCGGGTTTTCGCTTGTCATGCTTTTCTCTCCAGTCGCACCGGCACCACCGAATACGGGTTCTCTGCGAACGGCAAATTGa                                                                                                                                         <  1:18511/196‑1 (MQ=255)                                                                                                                                                                   cttcCGCTACGGTAACGAGAGGTTCGTCCGGGGCTTCTGACGGGGTTTCCGTCGCCGTGTCGGCGGCAATCTGCGGTTCGTCGCTTTCTGTGTTTTCTATTGAAAATCTCTCGGTCGGGTTTTCGCTTGTCATGCTTTTCTCTCCAGTCGCACCGGCACCACCGAATACGGGTTCTCTGCGAACGGCAAATTGATTTTCCATATTCTTTTGTTCTTTCTTCGCAAGGAGATCTTTGCCTTTTCCAAGCAAc                                                                                <  1:499010/251‑1 (MQ=255)                                                                                                                                                                         cTACGGTAACGAGAGGTTCGTCCGGGGCTTCTGACGGGGTTTCCGTCGCCGTGTCGGCGGCAATCTGCGGTTCGTCGCTTTCTGTGTTTTCTATTGAAAATCTCTCGGTCGGGTTTTCGCTTGTCATGCTTTTCTCTCCAGTCGCACCGGCACCACCGAATACGGGTTCTCTGCGAACGGCAAATTGATTTTCCATATTCTTTTGTTCTTTCTTCGCAAGGAGATCTTTGCCTTTTCCAAGCAACTTCTca                                                                          >  1:842901/1‑251 (MQ=255)                                                                                                                                                                           aCGGTAACGAGAGGTTCGTCCGGGGCTTCTGACGGGGTTTCCGTCGCCGTGTCGGCGGCAATCTGCGGTTCGTCGCTTTCTGTGTTTTCTATTGAAAATCTCTCGGGCGGGTTTTCGCTTGTCATGCTTTTCTCTCCAGTCGCACCGGCACCACCGAATACGGGTTCTCTGCGAACGGCAAATTGATTTTCCATATTCTTTTGttctttc                                                                                                                 >  1:769654/1‑210 (MQ=255)                                                                                                                                                                            cGGTAACGAGAGGTTCGTCCGGGGCTTCTGACGGGGTTTCCGTCGCCGTGTCGGCGGCAATCTGCGGTTCGTCGCTTTCTGTGTTTTCTATTGAAAATCTCTCGGTCGGGTTTTCGCTTGTCATGCTTTTCTCTCCAGTCGCACCGGCACCACCGAATACGGGTTCTCTGCGAACGGCAAATTGATTTTCCATATTCTTTTGTTCTTTCTTCGCAAGGAGATCTTTGCCTTTTCCAAGCAACTTCTcaaac                                                                       <  1:358942/251‑1 (MQ=255)                                                                                                                                                                              gTAACGAGAGGTTCGTCCGGGGCTTCTGACGGGGTTTCCGTCGCCGTGTCGGCGGCAATCTGCGGTTCGTCGCTTTCTGTGTTTTCTATTGAAAATCTCTCGGTCGGGTTTTCGCTTGTCATGCTTTTCTCTCCAGTCGCACCGGCACCACCGAATACGGGTTCTCTGCGAACGGCAAATTGATTTTCCATATTCTTTTGTTCTTTCTTCGCAAGGAGATCTTTGcc                                                                                             >  1:730211/1‑227 (MQ=255)                                                                                                                                                                              gTAACGAGAGGTTCGTCCGGGGCTTCTGACGGGGTTTCCGTCGCCGTGTCGGCGGCAATCTGCGGTTCGTCGCTTTCTGTGTTTTCTATTGAAAATCTCTCGGTCGGGTTTTCGCTTGTCATGCTTTTCTCTCCAGTCGCACCGGCACCACCGAATACGGGTTCTCTGCGAACGGCAAATTGATTTTCCATATTCTTTTGTTCTTTCTTCGCAAGGAGATCTTTGCCTTTTCCAAGCAACTTCTcaaacaa                                                                     >  1:23828/1‑251 (MQ=255)                                                                                                                                                                              gTAACGAGAGGTTCGTCCGGGGCTTCTGACGGGGTTTCCGTCGCCGTGTCGGCGGCAATCTGCGGTTCGTCGCTTTCTGTGTTTTCTATTGAAAATCTCTCGGTCGGGTTTTCGCTTGTCATGCTTTTCTCTCCAGTCGCACCGGCACCACCGAATACGGGTTCTCTGCGAACGGCAAATTGATTTTCCATACTCTTTTGTTCTTTCTTCGCAAGGAGATCTTTGCCTTTTCCAAGCAACTTCTcaaacaa                                                                     >  1:855901/1‑251 (MQ=255)                                                                                                                                                                                aaCGAGAGGTTCGTCCGGGGCTTCTGACGGGGTTTCCGTCGCCGTGTCGGCGGCAATCTGCGGTTCGTCGCTTTCTGTGTTTTCTATTGAAAATCTCTCGGTCGGGTTTTCGCTTGTCATGCTTTTCTCTCCAGTCGCACCGGCACCACCGAATACGGGTTCTCTGCGAACGGCAAATTGATTTTCCATATTCTTTTGttc                                                                                                                     >  1:824310/1‑201 (MQ=255)                                                                                                                                                                                  cGAGAGGTTCGTCCGGGGCTTCTGACGGGGTTTCCGTCGCCGTGTCGGCGGCAATCTGCGGTTCGTCGCTTTCTGTGTTTTCTATTGAAAATCTCTCGGTCGGGTTTTCGCTTGTCATGCTTTTCTCTCCAGTCGCACCGGCACCACCGAATACGGGTTCTCTGCGAACGGCAAATTGATTTTCCATATTCTTTTGTTCTTTCTTCGCAAGGAGATCTTTGCCt                                                                                            <  1:77163/224‑1 (MQ=255)                                                                                                                                                                                  cGAGAGGTTCGTCCGGGGCTTCTGACGGGGTTTCCGTCGCCGTGTCGGCGGCAATCTGCGGTTCGTCGCTTTCTGTGTTTTCTATTGAAAATCTCTCGGTCGGGTTTTCGCTTGTCATGCTTTTCTCTCCAGTCGCACCGGCACCACCGAATACGGGTTCTCTGCGAACGGCAAATTGATTTTCCATATTCTTTTGTTCTTTCTTCGCAAGGAGATCTTTGCCTTTTCCAAGCAACTTCTCAAACAAAGAc                                                                 <  1:157338/251‑1 (MQ=255)                                                                                                                                                                                            gTCCGGGGCTTCTGACGGGGTTTCCGTCGCCGTGTCGGCGGCAATCTGCGGTTCGTCGCTTTCTGTGTTTTCTATTGAAAATCTCTCGGTCGGGTTTTCGCTTGTCATGCTTTTCTCTCCAGTCGCACCGGCACCACCGAATACGGGTTCTCTGCGAACGGCAAATTGATTTTCCATATTCTTTTGTTCTTTCTTCGCAAGGAGATCTTTGCCTTTTCCAAGCAACTTCTCAAACAAAGACGGTTCCGGtt                                                       >  1:27263/1‑251 (MQ=255)                                                                                                                                                                                             tCCGGGGCTTCTGACGGGGTTTCCGTCGCCGTGTCGGCGGCAATCTGCGGTTCGTCGCTTTCTGTGTTTTCTATTGAAAATCTCTCGGTCGGGTTTTCGCTTGTCATGCTTTTCTCTCCAGTCGCACCGGCACCACCGAATACGGGTTCTCTGCGAACGGCAAATTGATTTTCCATATTCTTTTGTTCTTTCTTCGCAAGGAGATCTTTGCCTTTTCCAAGCAACTTCTCAAACAAAGACGGTTCCGGtt                                                       <  1:824095/250‑1 (MQ=255)                                                                                                                                                                                             tCCGGGGCTTCTGACGGGGTTTCCGTCGCCGTGTCGGCGGCAATCTGCGGTTCGTCGCTTTCTGTGTTTTCTATTGAAAATCTCTCGGTCGGGTTTTCGCTTGTCATGCTTTTCTCTCCAGTCGCACCGGCACCACCGAATACGGGTTCTCTGCGAACGGCAAATTGATTTTCCATATTCTTTTGTTCTTTCTTCGCAAGGAGATCTTTGCCTTTTCCAAGCAACTTCTCAAACAAAGACGGTTCCGGtt                                                       <  1:573168/250‑1 (MQ=255)                                                                                                                                                                                                  ggCTTCTGACGGGGTTTCCGTCGCCGTGTCGGCGGCAATCTGCGGTTCGTCGCTTTCTGTGTTTTCTATTGAAAATCTCTCGGTCGGGTTTTCGCTTGTCATGCTTTTCTCTCCAGTCGCACCGGCACCACCGAATACGGGTtc                                                                                                                                                            >  1:240697/1‑144 (MQ=255)                                                                                                                                                                                                   gCTTCTGACGGGGTTTCCGTCGCCGTGTCGGCGGCAATCTGCGGTTCGTCGCTTTCTGTGTTTTCTATTGAAAATCTCTCGGTCGGGTTTTCGCTTGTCATGCTTTTCTCTCCAGTCGCACCGGCACCACCGAATACGGGTTCTCTGCGAACGGCAAATTGATTTTCCATATTCTTTTGTTCTTTCTTCGCAAGGAGATCTTTGCCTTTTCCAAGCAACTTCTCAAACAAAGACGGTTCCGGTTTAATCa                                                 >  1:295857/1‑250 (MQ=255)                                                                                                                                                                                                      tCTGACGGGGTTTCCGTCGCCGTGTCGGCGGCAATCTGCGGTTCGTCGCTTTCTGTGTTTTCTATTGAAAATCTCTCGGTCGGGTTTTCGCTTGTCATGCTTTTCTCTCCAGTCGCACCGGCACCACCGAATACGGGTTCTCTGCGAACGGCAAATTGATTTTCCATATTCTTTTGTTCTTTCTTCGCAAGGAGATCTTTGCCTTTTCCAAGCAACTTCTCAAACAAAGACGGTTCCGGTTTAATCACttt                                             <  1:281891/251‑1 (MQ=255)                                                                                                                                                                                                       cTGACGGGGTTTCCGTCGCCGTGTCGGCGGCAATCTGCGGTTCGTCGCTTTCTGTGTTTTCTATTGAAAATCTCTCGGTCGGGTTTTCGCTTGTCATGCTTTTCTCTCCAGTCGCACCGGCACCACCGAATACGGGTTCTCTGCGAACGGCAAATTGATTTTCCATATTCTTTTGTTCTTTCTTCGCAAGGAGATCTTTGCCTTTTCCAAGCAACTTCTCAAACAAAGACGGTTCCGGTTTAATCACtttt                                            >  1:104550/1‑251 (MQ=255)                                                                                                                                                                                                          aCGGGGTTTCCGTCGCCGTGTCGGCGGCAATCTGCGGTTCGTCGCTTTCTGTGTTTTCTATTGAAAATCTCTCGGTCGGGTTTTCGCTTGTCATGCTTTTCTCTCCAGTCGCACCGGCACCACCGAATACGGGTTCTCTGCGAACGGCAAATTGATTTTCCATATTCTTTTGTTCTTTCTTCGCAAGGAGATCTTTGCCTTTTCCAAGCAACTTCTCAAACAAAGACGGTTCCGGTTTAATCACTTTTTTc                                         <  1:614222/251‑1 (MQ=255)                                                                                                                                                                                                             gggTTTCCGTCGCCGTGTCGGCGGCAATCTGCGGTTCGTCGCTTTCTGTGTTTTCTATTGAAAATCTCTCGGTCGGGTTTTCGCTTGTCATGCTTTTCTCTCCAGTCGCACCGGCACCACCGAATAc                                                                                                                                                                  <  1:271091/127‑1 (MQ=255)                                                                                                                                                                                                             gggTTTCCGTCGCCGTGTCGGCGGCAATCTGCGGTTCGTCGCTTTCTGTGTTTTCTATTGAAAATCTCTCGGTCGGGTTTTCGCTTGTCATGCTTTTCTCTCCAGTCGCACCGGCACCACCGAATACGGGTTCTCTGCGAACGGCAAATTGATTTTCCATATTCTTTTGTTCTTTCTTCGCAAGGAGATCTTTGCCTTTTCCAAGCAACTTCTCAAACAAAGACGGTTCCGGTTTAATCACTTTTTTCggg                                      >  1:23999/1‑251 (MQ=255)                                                                                                                                                                                                              ggTTTCCGTCGCCGTGTCGGCGGCAATCTGCGGTTCGTCGCTTTCTGTGTTTTCTATTGAAAATCTCTCGGTCGGGTTTTCGCTTGTCATGCTTTTCTCTCCAGTCGCACCGGcac                                                                                                                                                                            <  1:880892/116‑1 (MQ=255)                                                                                                                                                                                                                tttCCGTCGCCGTGTCGGCGGCAATCTGCGGTTCGTCGCTTTCTGTGTTTTCTATTGAAAATCTCTCGGTCGGGTTTTCGCTTGTCATGCTTTTCTCTCCAGTCGCACCGGCACCACCGAATACGGGTTCTCTGCGAACGGCAAATTGATTTTCCATATTCTTTTGTTCTTTCTTCGCAAGGAGATCTTTGCCTTTTCCAAGCAACTTCTCAAACAAAGACGGTTCCGGTTTAATCACTTTTTTCGGGGt                                    <  1:502010/250‑1 (MQ=255)                                                                                                                                                                                                                      tCGCCGTGTCGGCGGCAATCTGCGGTTCGTCGCTTTCTGTGTTTTCTATTGAAAATCTCTCGGTCGGGTTTTCGCTTGTCATGCTTTTCTCTCCAGTCGCACCGGCACCACCGAATACGGGTTCTCTGCGAACGGCAAATTGATTTTCCATATTCTTTTGTTCTTTCTTCGCAAGGAGATCTTTGCCTTTTCCAAGCAACTTCTCAAACAAAGACGGTTCCGGTTTAATCACTTTTTTCGGGGTGATCGGt                             <  1:187110/251‑1 (MQ=255)                                                                                                                                                                                                                       cGCCGTGTCGGCGGCAATCTGCGGTTCGTCGCTTTCTGTGTTTTCTATTGAAAATCTCTCGGTCGGGTTTTCGCTTGTCATGCTTTTCTCTCCAGTCGCACCGGCACCACCGAATACGGGTTCTCTGCGAACGGCAAATTGATTTTCCATATTCTTTTGTTCTTTCTTCGCAAGGAGATCttt                                                                                                <  1:782712/183‑1 (MQ=255)                                                                                                                                                                                                                                      aaTCTGCGGTTCGTCGCTTTCTGTGTTTTCTATTGAAAATCTCTCGGTCGGGTTTTCGCTTGTCATGCTTTTCTCTCCAGTCGCACCGGCACCACCGAATACGGGTTCTCTGCGAACGGCAAATTGATTTTCCATATTCTTTTGTTCTTTCTTCGCAAGGAGATCTTTGCCTTTTCCAAGCAACTTCTCAAACAAAGACGGTTCCGGTTTAATCACTTTTTTCGGGGTGATCGGTTCCATTTGACTAAAGc             <  1:96871/251‑1 (MQ=255)                                                                                                                                                                                                                                       aTCTGCGGTTCGTCGCTTTCTGTGTTTTCTATTGAAAATCTCTCGGTCGGGTTTTCGCTTGTCATGCTTTTCTCTCCAGTCGCACCGGCACCACCGAATACGGGTTCTCTGCGAACGGCAAATTGATTTTCCATATTCTTTTGTTCTTTCTTCGCAAGGAGATCTTTGCCTTTTCCAAGCAACTTCTCAAACAAAGACGGTTCCGGTTTAATCACTTTTTTCGGGGTGATCGGTTCCATTTGACt                  >  1:748174/1‑245 (MQ=255)                                                                                                                                                                                                                                       aTCTGCGGTTCGTCGCTTTCTGTGTTTTCTATTGAAAATCTCTCGGTCGGGTTTTCGCTTGTCATGCTTTTCTCTCCAGTCGCACCGGCACCACCGAATACGGGTTCTCTGCGAACGGCAAATTGATTTTCCATATTCTTTTGTTCTTTCTTCGCAAGGAGATCTTTGCCTTTTCCAAGCAACTTCTCAAACAAAGACGGTTCCGGTTTAATCACTTTTTTCGGGGTGATCGGTTCCATTTGACTAAAGcc            <  1:670221/251‑1 (MQ=255)                                                                                                                                                                                                                                       aTCTGCGGTTCGTCGCTTTCTGTGTTTTCTATTGAAAATCTCTCGGTCGGGTTTTCGCTTGTCATGCTTTTCTCTCCAGTCGCACCGGCACCACCGAATACGGGTTCTCTGCGAACGGCAAATTGATTTTCCATATTCTTTTGTTCTTTCTTCGCAAGGAGATCTTTGCCTTTTCCAAGCAACTTCTCAAACAAAGACGGTTCCGGTTTAATCACTTTTTTCGGGGTGATCGGTTCCATTTGACTAAAGcc            <  1:389135/251‑1 (MQ=255)                                                                                                                                                                                                                                              gTTCGTCGCTTTCTGTGTTTTCTATTGAAAATCTCTCGGTCGGGTTTTCGCTTGTCATGCTTTTCTCTCCAGTCGCACCGGCACCACCGAATACGGGTTCTCTGCGAACGGCAAATTGATTTTCCATATTCTTTTGTTCTTTCTTCGCAAGGAGATCTTTGCCTTTTCCAAGCAACTTCTCAAACAAAGACGGTTCCGGTTTAATCACTTTTTTCGGGGTGATCGGTTCCATTTGACTAAAGCCCAAATc      <  1:409902/250‑1 (MQ=255)                                                                                                                                                                                                                                              gTTCGTCGCTTTCTGTGTTTTCTATTGAAAATCTCTCGGTCGGGTTTTCGCTTGTCATGCTTTTCTCTCCAGTCGCACCGGCACCACCGAATACGGGTTCTCTGCGAACGGCAAATTGATTTTCCATATTCTTTTGTTCTTTCTTCGCAAGGAGATCTTTGCCTTTTCCAAGCAACTTCTCAAACAAAGACGGTTCCGGTTTAATCACTTTTTTCGGGGTGATCGGTTCCATTTGACTAAAGCCCAAATc      >  1:585680/1‑250 (MQ=255)                                                                                                                                                                                                                                                tcgtcgCTTTCTGTGTTTTCTATTGAAAATCTCTCGGTCGGGTTTTCGCTTGTCATGCTTTTCTCTCCAGTCGCACCGGCACCACCGAATACGGGTTCTCTGCGAACGGCAAATTGATTTTCCATATTCTTTTGTTCTTTCTTCGCAAGGAGATCTTTGCCTTTTCCAAGCAACTTCTCAAACAAAGACGGTTCCGGTTTAATCACTTTTTTCGGGGTGATCGGTTc                           <  1:70430/227‑1 (MQ=255)                                                                                                                                                                                                                                                tcgtcgCTTTCTGTGTTTTCTATTGAAAATCTCTCGGTCGGG‑TTTCGCTTGTCATGCTTTTCTCTCCAGTCGCACCGGCACCACCGAATACGGGTTCTCTGCGAACGGCAAATTGATTTTCCATATTCTTTTGTTCTTTCTTCGCAAGGAGATCTTTGCCTTTTCCAAGCAACTTCTCAAACAAAGACGGTTCCGGTTTAATCACTTTTTTCGGGGTGATCGGTTCCATTTGACTAAAGCCCAAATCCAAt  <  1:588547/251‑1 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                        AGATAAAAATCAGCACCAACACCACGCCGAATAGCGCGACGAAAATGCGACGATGTTTTTGCGGGAGAATACTTAAAATCGCCCAATTTTCAGGATTTTTAAAACCGGCTTTCGGTTTTTCTTTCAATTCGGCAGGTTCCGGTTGTGTTGCTTCAACGGCTTCTTCCGCTACGGTAACGAGAGGTTCGTCCGGGGCTTCTGACGGGGTTTCCGTCGCCGTGTCGGCGGCAATCTGCGGTTCGTTGCTTTCTGTGTTTTCTATTGAAAATCTCTCGGTCGGGTTTTCGCTTGTCATGCTTTTCTCTCCAGTCGCACCGGCACCACCGAATACGGGTTCTCTGCGAACGGCAAATTGATTTTCCATATTCTTTTGTTCTTTCTTCGTAAGGAGATCTTTGCCTTTTCCAAGCAACTTCTCAAACAAAGACGGTTCCGGTTTAATCACTTTTTTCGGGGTGATCGGTTCCATTTGACTAAAGCCCAAATCCAAT  >  1/2079078‑2079568

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑