BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	2,072,255	A→G	intergenic (+132/+337)	unknown → / ← unknown	4‑hydroxy‑3‑methylbut‑2‑enyl diphosphate reductase (EC 1.17.7.4)/Cell division trigger factor (EC 5.2.1.8)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	2,072,255	0	A	G	100.0%	181.9 / NA	64	intergenic (+132/+337)	unknown/unknown	4‑hydroxy‑3‑methylbut‑2‑enyl diphosphate reductase (EC 1.17.7.4)/Cell division trigger factor (EC 5.2.1.8)
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (35/29); total (35/29)

GTTATCTCCCGCCTGAAAGAATTCGGCGTCACCACCGTAGAAGAACTGCAAGGCTTGGAAGAAAATATGTTCTTTGAAGTTCCGAAAGAGTTGCGGCTGAAAGAAGCAAATTGATTTTTCACCGAATGTATTTCCGAGAATTTGATGGTGCTCATCTGATGGCGCGCATTTCCCAATTATCGCTGTCGAAACATTGTAGTTATTTAATAAAAAAGAAAAATTTGAATCCTCTCGTTTACGGGGGAACCGGACGAAACCCTGAGAGGGCAAAATCTCGATTTTTAAGCATGAGTTTGTTAAAAAATAGAGTATATTCCCCGCCCTTCGGGCACCTTCCCCCGTAAACGGAAGAAGGCAAGATACTTTATAAAATATCAATGCATTACAATTTTTCGAACACTTGTGGTTTCAACACGTGCTTTTTGTTCCTATTAGTACTGCGACGTGTACGCTATCTATCAAGT  >  1/2072010‑2072473

gTTATCTCCCGCCTGAAAGAATTCGGCGTCACCACCGTAGAAGAACTGCAAGGCTTGGAAGAAAATATGTTCTTTGAAGTTCCGAAAGAGTTGCGGCTGAAAGAAGCAAATTGATTTTTCACCGAATGTATTTCCGAGAATTTGATGGTGCTCATCTGATGGCGCGCATTTCCCAATTATCGCTGTCGAAACATTGTAGTTATTTAATAAAAAAGAAAAATTTGAATCCTCTCGTTTACGGGGGAGCCGGa                                                                                                                                                                                                                       >  1:681567/1‑251 (MQ=255)

    tctcCCGCCTGAAAGAATTCGGCGTCACCACCGTAGAAGAACTGCAAGGCTTGGAAGAAAATATGTTCTTTGAAGTTCCGAAAGAGTTGCGGCTGAAAGAAGCAAATTGATTTTTCACCGAATGTATTTCCGAGAATTTGATGGTGCTCATCTGATGGCGCGCATTTCCCAATTATCGCTGTCGAAACATTGTAGTTATTTAATAAAAAAGAAAAATTTGAATCCTCTCGTTTACGGGGGAGCCGGACGaa                                                                                                                                                                                                                   >  1:328036/1‑251 (MQ=255)

    tctcCCGCCTGAAAGAATTCGGCGTCACCACCGTAGAAGAACTGCAAGGCTTGGAAGAAAATATGTTCTTTGAAGTTCCGAAAGAGTTGCGGCTGAAAGAAGCAAATTGATTTTTCACCGAATGTATTTCCGAGAATTTGATGGTGCTCATCTGATGGCGCGCATTTCCCAATTATCGCTGTCGAAACATTGTAGTTATTTAATAAAAAAGAAAAATTTGAATCCTCTCGTTTACGGGGGAGCCGGACGaa                                                                                                                                                                                                                   >  1:324124/1‑251 (MQ=255)

             tGAAAGAATTCGGCGTCACCACCGTAGAAGAACTGCAAGGCTTGGAAGAAAATATGTTCTTTGAAGTTCCGAAAGAGTTGCGGCTGAAAGAAGCAAATTGATTTTTCACCGAATGTATTTCCGAGAATTTGATGGTGCTCATCTGATGGCGCGCATTTCCCAATTATCGCTGTCGAAACATTGTAGTTATTTAATAAAAAAGAAAAATTTGAATCCTCTCGTTTACGGGGGAGCCGGACGAAACCCTgaga                                                                                                                                                                                                          <  1:242850/251‑1 (MQ=255)

                      tCGGCGTCACCACCGTAGAAGAACTGCAAGGCTTGGAAGAAAATATGTTCTTTGAAGTTCCGAAAGAGTTGCGGCTGAAAGAAGCAAATTGATTTTTCACCGAATGTATTTCCGAGAATTTGATGGTGCTCATCTGATGGCGCGCATTTCCCAATTATCGCTGTCGAAACATTGTAGTTATTTAATAAAAAAGAAAAATTTGAATCCTCTCGTTTACGGGGGAGCCGGACGAAACCCTGAGAGGGCAAAAt                                                                                                                                                                                                 <  1:256834/251‑1 (MQ=255)

                                  ccGTAGAAGAACTGCAAGGCTTGGAAGAAAATATGTTCTTTGAAGTTCCGAAAGAGTTGCGGCTGAAAGAAGCAAATTGATTTTTCACCGAATGTATTTCCGAGAATTTGATGGTGCTCATCTGATGGCGCGCATTTCCCAATTATCGCTGTCGAAACATTGTAGTTATTTAATAAAAAAGAAAAATTTGAATCCTCTCGTTTACGGGGGAGCCGGACGAAACCCTGagag                                                                                                                                                                                                         <  1:616213/231‑1 (MQ=255)

                                  ccGTAGAAGAACTGCAAGGCTTGGAAGAAAATATGTTCTTTGAAGTTCCGAAAGAGTTGCGGCTGAAAGAAGCAAATTGATTTTTCACCGAATGTATTTCCGAGAATTTGATGGTGCTCATCTGATGGCGCGCATTTCCCAATTATCGCTGTCGAAACATTGTAGTTATTTAATAAAAAAGAAAAATTTGAATCCTCTCGTTTACGGGGGAGCCGGACGAAACCCTGAGAGGGCAAAATCTCGAt                                                                                                                                                                                           >  1:685077/1‑245 (MQ=255)

                                  ccGTAGAAGAACTGCAAGGCTTGGAAGAAAATATGTTCTTTGAAGTTCCGAAAGAGTTGCGGCTGAAAGAAGCAAATTGATTTTTCACCGAATGTATTTCCGAGAATTTGATGGTGCTCATCTGATGGCGCGCATTTCCCAATTATCGCTGTCGAAACATTGTAGTTATTTAATAAAAAAGAAAAATTTGAATCCTCTCGTTTACGGGGGAGCCGGACGAAACCCTGAGAGGGCAAAATCTCGATTTTTaa                                                                                                                                                                                     >  1:802835/1‑251 (MQ=255)

                                    gTAGAAGAACTGCAAGGCTTGGAAGAAAATATGTTCTTTGAAGTTCCGAAAGAGTTGCGGCTGAAAGAAGCAAATTGATTTTTCACCGAATGTATTTCCGAGAATTTGATGGTGCTCATCTGATGGCGCGCATTTCCCAATTATCGCTGTCGAAACATTGTAGTTATTTAATAAAAAAGAAAAATTTGAATCCTCTCGTTTACGGGGGAGCCGGACGAAACCCTGAGAGGGCAAAATCTCGATTTTTAAg                                                                                                                                                                                    >  1:113455/1‑250 (MQ=255)

                                     tAGAAGAACTGCAAGGCTTGGAAGAAAATATGTTCTTTGAAGTTCCGAAAGAGTTGCGGCTGAAAGAAGCAAATTGATTTTTCACCGAATGTATTTCCGAGAATTTGATGGTGCTCATCTGATGGCGCGCATTTCCCAATTATCGCTGTCGAAACATTGTAGTTATTTAATAAAAAAGAAAAATTTGAATCCTCTCGTTTACGGGGGAGCCGGACGAAACCCTGAGAGGGCAAAATCTCGATTTTTAAGCa                                                                                                                                                                                  >  1:187191/1‑251 (MQ=255)

                                     tAGAAGAACTGCAAGGCTTGGAAGAAAATATGTTCTTTGAAGTTCCGAAAGAGTTGCGGCTGAAAGAAGCAAATTGATTTTTCACCGAATGTATTTCCGAGAATTTGATGGTGCTCATCTGATGGCGCGCATTTCCCAATTATCGCTGTCGAAACATTGTAGTTATTTAATAAAAAAGAAAAATTTGAATCCTCTCGTTTACGGGGGAGCCGGACGAAACCCTGAGAGGGCAAAATCTCGATTTTTAAGCa                                                                                                                                                                                  >  1:35307/1‑251 (MQ=255)

                                          gaaCTGCAAGGCTTGGAAGAAAATATGTTCTTTGAAGTTCCGAAAGAGTTGCGGCTGAAAGAAGCAAATTGATTTTTCACCGAATGTATTTCCGAGAATTTGATGGTGCTCATCTGATGGCGCGCATTTCCCAATTATCGCTGTCGAAACATTGTAGTTATTTAATAAAAAAGAAAAATTTGAATCCTCTCGTTTACGGGGGAGCCGGACGAAACCCTGAGAGGGCAAAATCTCGATTTTTAAGCATGAGt                                                                                                                                                                             >  1:401311/1‑251 (MQ=255)

                                           aaCTGCAAGGCTTGGAAGAAAATATGTTCTTTGAAGTTCCGAAAGAGTTGCGGCTGAAAGAAGCAAATTGATTTTTCACCGAATGTATTTCCGAGAATTTGATGGTGCTCATCTGATGGCGCGCATTTCCCAATTATCGCTGTCGAAACATTGTAGTTATTTAATAAAAAAGAAAAATTTGAATCCTCTCGTTTACGGGGGAGCCGGACGAAACCCTGAGAGGGCAAAATCTCGATTTTTAAGCATGAGtt                                                                                                                                                                            <  1:219497/251‑1 (MQ=255)

                                             cTGCAAGGCTTGGAAGAAAATATGTTCTTTGAAGTTCCGAAAGAGTTGCGGCTGAAAGAAGCAAATTGATTTTTCACCGAATGTATTTCCGAGAATTTGATGGTGCTCATCTGATGGCGCGCATTTCCCAATTATCGCTGTCGAAACATTGTAGTTATTTAATAAAAAAGAAAAATTTGAATCCTCTCGTTTACGGGGGAGCCGGACGAAACCCTGAGAGGGCAAAATCTCGATTTTTAAGCATGAGTTTg                                                                                                                                                                          >  1:625429/1‑251 (MQ=255)

                                                    gCTTGGAAGAAAATATGTTCTTTGAAGTTCCGAAAGAGTTGCGGCTGAAAGAAGCAAATTGATTTTTCACCGAATGTATTTCCGAGAATTTGATGGTGCTCATCTGATGGCGCGCATTTCCCAATTATCGCTGTCGAAACATTGTAGTTATTTAATAAAAAAGAAAAATTTGAATCCTCTCGTTTACGGGGGAGCCGGACGAAACCCTGAGAGGGCAAAATCTCGATTTTTAAGCATGAGTTTGTTaaaaa                                                                                                                                                                   >  1:20184/1‑251 (MQ=255)

                                                    gCTTGGAAGAAAATATGTTCTTTGAAGTTCCGAAAGAGTTGCGGCTGAAAGAAGCAAATTGATTTTTCACCGAATGTATTTCCGAGAATTTGATGGTGCTCATCTGATGGCGCGCATTTCCCAATTATCGCTGTCGAAACATTGTAGTTATTTAATAAAAAAGAAAAATTTGAATCCTCTCGTTTACGGGGGAGCCGGACGAAACCCTGAGAGGGCAAAATCTCGATTTTTAAGCATGAGTTTGTTaaaaa                                                                                                                                                                   >  1:313360/1‑251 (MQ=255)

                                                             aaaaTATGTTCTTTGAAGTTCCGAAAGAGTTGCGGCTGAAAGAAGCAAATTGATTTTTCACCGAATGTATTTCCGAGAATTTGATGGTGCTCATCTGATGGCGCGCATTTCCCAATTATCGCTGTCGAAACATTGTAGTTATTTAATAAAAAAGAAAAATTTGAATCCTCTCGTTTACGGGGGAGCCGGACGAAACCCTGAGAGGGCAAAATCTCGATTTTTAAGCATGAGTTTGTTAAAAAATAGAGtat                                                                                                                                                          <  1:390121/251‑1 (MQ=255)

                                                                  atGTTCTTTGAAGTTCCGAAAGAGTTGCGGCTGAAAGAAGCAAATTGATTTTTCACCGAATGTATTTCCGAGAATTTGATGGTGCTCATCTGATGGCGCGCATTTCCCAATTATCGCTGTCGAAACATTGTAGTTATTTAATAAAAAAGAAAAATTTGAATCCTCTCGTTTACGGGGGAGCCGGACGAAACCCTGAGAGGGCAAAATCTCGATTTTTAAGCATGAGTTTGTTAAAAAATAGAGTATATTcc                                                                                                                                                     <  1:87870/251‑1 (MQ=255)

                                                                  atGTTCTTTGAAGTTCCGAAAGAGTTGCGGCTGAAAGAAGCAAATTGATTTTTCACCGAATGTATTTCCGAGAATTTGATGGTGCTCATCTGATGGCGCGCATTTCCCAATTATCGCTGTCGAAACATTGTAGTTATTTAATAAAAAAGAAAAATTTGAATCCTCTCGTTTACGGGGGAGCCGGACGAAACCCTGAGAGGGCAAAATCTCGATTTTTAAGCATGAGTTTGTTAAAAAATAGAGTATATTcc                                                                                                                                                     <  1:393496/251‑1 (MQ=255)

                                                                      tCTTTGAAGTTCCGAAAGAGTTGCGGCTGAAAGAAGCAAATTGATTTTTCACCGAATGTATTTCCGAGAATTTGATGGTGCTCATCTGATGGCGCGCATTTCCCAATTATCGCTGTCGAAACATTGTAGTTATTTAATAAAAAAGAAAAATTTGAATCCTCTCGTTTACGGGGGAGCCGGACGAAACCCTGAGAGGGCAAAATCTCGATTTTTAAGCATGAGTTTGTTAAAAAATAGAGTATATTCCCCg                                                                                                                                                  >  1:827531/1‑250 (MQ=255)

                                                                       cTTTGAAGTTCCGAAAGAGTTGCGGCTGAAAGAAGCAAATTGATTTTTCACCGAATGTATTTCCGAGAATTTGATGGTGCTCATCTGATGGCGCGCATTTCCCAATTATCGCTGTCGAAACATTGTAGTTATTTAATAAAAAAGAAAAATTTGAATCCTCTCGTTTACGGGGGAGCCGGACGAAACCCTGagag                                                                                                                                                                                                         >  1:735751/1‑194 (MQ=255)

                                                                              gTTCCGAAAGAGTTGCGGCTGAAAGAAGCAAATTGATTTTTCACCGAATGTATTTCCGAGAATTTGATGGTGCTCATCTGATGGCGCGCATTTCCCAATTATCGCTGTCGAAACATTGTAGTTATTTAATAAAAAAGAAAAATTTGAATCCTCTCGTTTACGGGGGAGCCGGACGAAACCCTGAGAGGGCAAAATCTCGATTTTTAAg                                                                                                                                                                                    >  1:148457/1‑208 (MQ=255)

                                                                                             cGGCTGAAAGAAGCAAATTGATTTTTCACCGAATGTATTTCCGAGAATTTGATGGTGCTCATCTGATGGCGCGCATTTCCCAATTATCGCTGTCGAAACATCGTAGTTATTTAATAAAAAAGAAAAATTTGAATCCTCTCGTTTACGGGGGAGCCGGACGAAACCCTGAGAGGGCAAAATCTCGATTTTTAAGCATGAGTTTGTTAAAAAATAGAGTATATTCCCCGCCCTTCGGGCACCTTCCCCCGTa                                                                                                                           <  1:292767/250‑1 (MQ=255)

                                                                                                  gAAAGAAGCAAATTGATTTTTCACCGAATGTATTTCCGAGAATTTGATGGTGCTCATCTGATGGCGCGCATTTCCCAATTATCGCTGTCGAAACATTGTAGTTATTTAATAAAAAAGAAAAATTTGAATCCTCTCGTTTACGGGGGAGCCGGACGAAACCCTGAGAGGGCAAAATCTCGATTTTTAAGc                                                                                                                                                                                   >  1:56125/1‑189 (MQ=255)

                                                                                                  gAAAGAAGCAAATTGATTTTTCACCGAATGTATTTCCGAGAATTTGATGGTGCTCATCTGATGGCGCGCATTTCCCAATTATCGCTGTCGAAACATTGTAGTTATTTAATAAAAAAGAAAAATTTGAATCCTCTCGTTTACGGGGGAGCCGGACGAAACCCTGAGAGGGCAAAATCTCGATTTTTAAGCATGAGTTTGTTAAAAAATAGAGTATATTCCCCGCCCTTCGGGCACCTTCCCCCGTAAACGga                                                                                                                     >  1:347013/1‑251 (MQ=255)

                                                                                                  gAAAGAAGCAAATTGATTTTTCACCGAATGTATTTCCGAGAATTTGATGGTGCTCATCTGATGGCGCGCATTTCCCAATTATCGCTGTCGAAACATTGTAGTTATTTAATAAAAAAGAAAAATTTGAATCCTCTCGTTTACGGGGGAGCCGGACGAAACCCTGAGAGGGCAAAATCTCGATTTTTAAGCATGAGTTTGTTAAAAAATAGAGTATATTCCCCGCCCTTCGGGCACCTTCCCCCGTAAACGga                                                                                                                     >  1:153703/1‑251 (MQ=255)

                                                                                                    aagaagCAAATTGATTTTTCACCGAATGTATTTCCGAGAATTTGATGGTGCTCATCTGATGGCGCGCATTTCCCAATTATCGCTGTCGAAACATTGTAGTTATTTAATAAAAAAGAAAAATTTGAATCCTCTCGTTTACGGGGGAGCCGGACGAAACCCTGAGAGGGCAAAATCTCGATTTTTAAGCATGAGTTTGTTAAAAAATAGAGTATATTCCCCGCCCTTCGGGCACCTTCCCCCGTAAACGgaag                                                                                                                   <  1:330734/251‑1 (MQ=255)

                                                                                                          cAAATTGATTTTTCACCGAATGTATTTCCGAGAATTTGATGGTGCTCATCTGATGGCGCGCATTTCCCAATTATCGCTGTCGAAACATTGTAGTTATTTAATAAAAAAGAAAAATTTGAATCCTCTCGTTTACGGGGGAGCCGGACGAAACCCTGAGAGGGCAAAATCTCGATTTTTAAGCATGAGTTTGTTAAAAAATAGAGTATATTCCCCGCCCTTCGGGCACCCTCCCCCGTAAACGGAAGAAGGc                                                                                                              <  1:218369/250‑1 (MQ=255)

                                                                                                                       cACCGAATGTATTTCCGAGAATTTGATGGTGCTCATCTGATGGCGCGCATTTCCCAATTATCGCTGTCGAAACATTGTAGTTATTTAATAAAAAAGAAAAATTTGAATCCTCTCGTTTACGGGGGAGCCGGACGAAACCCTGAGAGGGCAAAATCTCGATTTTTAAGCATGAGTTTGTTAAAAAATAGAGTATATTCCCCGCCCTTCGGGCACCTTCCCCCGTAAACGGAAGAAGGCAAGATACTTTATaa                                                                                                <  1:656036/251‑1 (MQ=255)

                                                                                                                         ccGAATGTATTTCCGAGAATTTGATGGTGCTCATCTGATGGCGCGCATTTCCCAATTATCGCTGTCGAAACATTGTAGTTATTTAATAAAAAAGAAAAATTTGAATCCTCTCGTTTACGGGGGAGCCGGACGAAACCCTGAGAGGGCAAAATCTCGATTTTTAAGCATGAGTTTGTTAAAAAATAGAGTATATTCCCCGCCCTTCGGGCACCTTCCCCCGTAAACGGAAGAAGGCAAGATACTTTATaaaa                                                                                              >  1:345528/1‑251 (MQ=255)

                                                                                                                            aaTGTATTTCCGAGAATTTGATGGTGCTCATCTGATGGCGCGCATTTCCCAATTATCGCTGTCGAAACATTGTAGTTATTTAATAAAAAAGAAAAATTTGAATCCTCTCGTTTACGGGGGAGCCGGACGAAACCCTGAGAGGGCAAAATCTCGATTTTTAAGCATGAGTTTGTTAAAAAATAGAGTATATTCCCCGCCCTTCGGGCACCTTCCCCCGTAAACGGAAGAAGGCAAGATACTTTATAAAatat                                                                                           <  1:778334/251‑1 (MQ=255)

                                                                                                                             aTGTATTTCCGAGAATTTGATGGTGCTCATCTGATGGCGCGCATTTCCCAATTATCGCTGTCGAAACATTGTAGTTATTTAATAAAAAAGAAAAATTTGAATCCTCTCGTTTACGGGGGAGCCGGACGAAACCCTGAGAGGGCAAAATCTCGATTTTTAAGCATGAGTTTGTTAAAAAATAGAGTATATTCCCCGCCCTTCGGGCACCTTCCCCCGTa                                                                                                                           >  1:863494/1‑218 (MQ=255)

                                                                                                                                tATTTCCGAGAATTTGATGGTGCTCATCTGATGGCGCGCATTTCCCAATTATCGCTGTCGAAACATTGTAGTTATTTAATAAAAAAGAAAAATTTGAATCCTCTCGTTTACGGGGGAGCCGGACGAAACCCTGAGAGGGCAAAATCTCGATTTTTAAGCATGAGTTTGTTAAAAAATAGAGTATATTCCCCGCCCTTCGGGCACCTTCCCCCGTAAACGGAAGAAGGCAAGATACTTTATAAAATATCAAt                                                                                       <  1:753952/251‑1 (MQ=255)

                                                                                                                                          aaTTTGATGGTGCTCATCTGATGGCGCGCATTTCCCAATTATCGCTGTCGAAACATTGTAGTTATTTAATAAAAAAGAAAAATTTGAATCCTCTCGTTTACGGGGGAGCCGGACGAAACCCTGAGAGGGCAAAATCTCGATTTTTAAGCATGAGTTTGTTAAAAAATAGAGTATATTCCCCGCCCTTCGGGCACCTTCCCCCGTAAACGGAAGAAGGCAAGATACTTTATAAAATATCAATGCATTACaa                                                                              <  1:129443/250‑1 (MQ=255)

                                                                                                                                           aTTTGATGGTGCTCATCTGATGGCGCGCATTTCCCAATTATCGCTGTCGAAACATTGTAGTTATTTAATAAAAAAGAAAAATTTGAATCCTCTCGTTTACGGGGGAGCCGGACGAAACCCTGAGAGGGCAAAATCTCGATTTTTAAGCATGAGTTTGTTa                                                                                                                                                                       >  1:626726/1‑160 (MQ=255)

                                                                                                                                           aTTTGATGGTGCTCATCTGATGGCGCGCATTTCCCAATTATCGCTGTCGAAACATTGTAGTTATTTAATAAAAAAGAAAAATTTGAATCCTCTCGTTTACGGGGGAGCCGGACGAAACCCTGAGAGGGCAAAATCTCGATTTTTAAGCATGAGTTTGTTAAAAAATAGAGTATATTCCCCGCCCTTCGGGCACCTTCCCCCGTAAACGGAAGAAGGCAAGATACTTTATAAAATATCAATGCATTACAAtt                                                                            >  1:227271/1‑251 (MQ=255)

                                                                                                                                            tttGATGGTGCTCATCTGATGGCGCGCATTTCCCAATTATCGCTGTCGAAACATTGTAGTTATTTAATAAAAAAGAAAAATTTGAATCCTCTCGTTTACGGGGGAGCCGGACGAAACCCTGAGAGGGCAAAATCTCGATTTTTAAGCATGAGTTTGTTAAAAAATAGAGTATATTCCCCGCCCTTCGGGCACCTTCCCCCGTAAACGGAAGAAGGCAAGATACTTTATAAAATATCAATGCATTACAAttt                                                                           <  1:875922/251‑1 (MQ=255)

                                                                                                                                                   gTGCTCATCTGATGGCGCGCATTTCCCAATTATCGCTGTCGAAACATTGTAGTTATTTAATAAAAAAGAAAAATTTGAATCCTCTCGTTTACGGGGGAGCCGGACGAAACCCTGAGAGGGCAAAATCTCGATTTTTAAGCATGAGTTTGTTAAAAAATAGAGTATATTCCCCGCCCTTCGGGCACCTTCCCCCGTAAACGGAAGAAGGCAAGATACTTTATAAAATATCAATGCATTACAATTTTTCGaa                                                                     >  1:203823/1‑250 (MQ=255)

                                                                                                                                                   gTGCTCATCTGATGGCGCGCATTTCCCAATTATCGCTGTCGAAACATTGTAGTTATTTAATAAAAAAGAAAAATTTGAATCCTCTCGTTTACGGGGGAGCCGGACGAAACCCTGAGAGGGCAAAATCTCGATTTTTAAGCATGAGTTTGTTAAAAAATAGAGTATATTCCCCGCCCTTCGGGCACCTTCCCCCGTAAACGGAAGAAGGCAAGATACTTTATAAAATATCAATGCATTACAATTTTTCGaa                                                                     >  1:433487/1‑250 (MQ=255)

                                                                                                                                                          tCTGATGGCGCGCATTTCCCAATTATCGCTGTCGAAACATTGTAGTTATTTAATAAAAAAGAAAAATTTGAATCCTCTCGTTTACGGGGGAGCCGGACGAAACCCTGAGAGGGCAAAATCTCGATTTTTAAGCATGAGTTTGTTAAAAAATAGAGTATATTCCCCGCCCTTCGGGCACCTTCCCCCGTAAACGGAAGAAGGCAAGATACTTTATAAAATATCAATGCATTACAATTTTTCGAACACTtgtg                                                             >  1:667996/1‑251 (MQ=255)

                                                                                                                                                                          tCCCAATTATCGCTGTCGAAACATTGTAGTTATTTAATAAAAAAGAAAAATTTGAATCCTCTCGTTTACGGGGGAGCCGGACGAAACCCTGAGAGGGCAAAATCTCGATTTTTAAGCATGAGTTTGTTAAAAAATagag                                                                                                                                                             <  1:551271/139‑1 (MQ=255)

                                                                                                                                                                             cAATTATCGCTGTCGAAACATTGTAGTTATTTAATAAAAAAGAAAAATTTGAATCCTCTCGTTTACGGGGGAGCCGGACGAAACCCTGAGAGGGCAAAATCTCGATTTTTAAGCATGAGTTTGTTAAAAAATAGAGTATATTCCCCGCCCTTCGGGCACCTTCCCCCGTAAACGGAAGAAGGCAAGATACTTTATaaa                                                                                               >  1:35911/1‑198 (MQ=255)

                                                                                                                                                                                           gAAACATTGTAGTTATTTAATAAAAAAGAAAAATTTGAATCCTCTCGTTTACGGGGGAGCCGGACGAAACCCTGAGAGGGCAAAATCTCGATTTTTAAGCATGAGTTTGTTAAAAAATAGAGTATATTcc                                                                                                                                                     <  1:147301/130‑1 (MQ=255)

                                                                                                                                                                                            aaaCATTGTAGTTATTTAATAAAAAAGAAAAATTTGAATCCTCTCGTTTACGGGGGAGCCGGACGAAACCCTGAGAGGGCAAAATCTCGATTTTTAAGCATGAGTTTGTTAAAAAATAGAGTATATTCCCCGCCCTTCGGGCACCTTCCCCCGTAAACGGAAGAAGGCAAGATACTTTATAAAATATCAATGCATTACAATTTTTCGAACACTTGTGGTTTCAACACGTGCTTTTTGTTCCTATTAGTAc                            <  1:465827/250‑1 (MQ=255)

                                                                                                                                                                                            aaaCATTGTAGTTATTTAATAAAAAAGAAAAATTTGAATCCTCTCGTTTACGGGGGAGCCGGACGAAACCCTGAGAGGGCAAAATCTCGATTTTTAAGCATGAGTTTGTTAAAAAATAGAGTATATTCCCCGCCCTTCGGGCACCTTCCCCCGTAAACGGAAGAAGGCAAGATACTTTATAAAATATCAATGCATTACAATTTTTCGAACACTTGTGGTTTCAACACGTGCTTTTTGTTCCTATTAGTAc                            <  1:460816/250‑1 (MQ=255)

                                                                                                                                                                                              aCATTGTAGTTATTTAATAAAAAAGAAAAATTTGAATCCTCTCGTTTACGGGGGAGCCGGACGAAACCCTGAGAGGGCAAAATCTCGATTTTTAAGCATGAGTTTGTTAAAAAATAGAGTATATTCCCCGCCCTTCGGGCACCTTCCCCCGTAAACGGAAGAAGGCAAGATACTTTATAAAATATCAATGCATTACAATTTTTCGAacac                                                                  <  1:576492/210‑1 (MQ=255)

                                                                                                                                                                                              aCATTGTAGTTATTTAATAAAAAAGAAAAATTTGAATCCTCTCGTTTACGGGGGAGCCGGACGAAACCCTGAGAGGGCAAAATCTCGATTTTTAAGCATGAGTTTGTTAAAAAATAGAGTATATTCCCCGCCCTTCGGGCACCTTCCCCCGTAAACGGAAGAAGGCAAGATACTTTATAAAATATCAATGCATTACAATTTTTCGAACACTTGTGGTTTCAACACGTGCTTTTTGTTCCTATTAGTACTg                          >  1:798239/1‑250 (MQ=255)

                                                                                                                                                                                               cATTGTAGTTATTTAATAAAAAAGAAAAATTTGAATCCTCTCGTTTACGGGGGAGCCGGACGAAACCCTGAGAGGGCAAAATCTCGATTTTTAAGCATGAGTTTGTTAAAAAATAGAGTATATTCCCCGCCCTTCGGGCACCTTCCCCCGTAAACGGAAGAAGGCAAGATACTTTATAAAATATCAATGCATTACAATTTTTCGAACACTTGTGGTTTCAACACGTGCTTTTTGTTCCTATTAGTAc                            <  1:845898/247‑1 (MQ=255)

                                                                                                                                                                                               cATTGTAGTTATTTAATAAAAAAGAAAAATTTGAATCCTCTCGTTTACGGGGGAGCCGGACGAAACCCTGAGAGGGCAAAATCTCGATTTTTAAGCATGAGTTTGTTAAAAAATAGAGTATATTCCCCGCCCTTCGGGCACCTTCCCCCGTAAACGGAAGAAGGCAAGATACTTTATAAAATATCAATGCATTACAATTTTTCGAACACTTGTGGTTTCAACACGTGCTTTTTGTTCCTATTAGTACTGCg                        >  1:219164/1‑251 (MQ=255)

                                                                                                                                                                                                 ttGTAGTTATTTAATAAAAAAGAAAAATTTGAATCCTCTCGTTTACGGGGGAGCCGGACGAAACCCTGAGAGGGCAAAATCTCGATTTTTAAGCATGAGTTTGTTAAAAAATAGAGTATATTccc                                                                                                                                                    >  1:510097/1‑125 (MQ=255)

                                                                                                                                                                                                         aTTTAATAAAAAAGAAAAATTTGAATCCTCTCGTTTACGGGGGAGCCGGACGAAACCCTGAGAGGGCAAAATCTCGATTTTTAAGCATGAGTTTGTTAAAAAATAGAGTATATTCCCCGCCCTTCGGGCACCTTCCCCCGTAAACGGAAGAAGGCAAGATACTTTATAAAATATCAATGCATTACAATTTTTCGAACACTTGTGGTTTCAACACGTGCTTTTTGTTCCTATTAGTACTGCGACGTGTACGc              <  1:560115/251‑1 (MQ=255)

                                                                                                                                                                                                                 aaaaaGAAAAATTTGAATCCTCTCGTTTACGGGGGAGCCGGACGAAACCCTGAGAGGGCAAAATCTCGATTTTTAAGCATGAGTTTGTTAAAAAATAGAGTATATTCCCCGCCCTTCGGGCACCTTCCCCCGTAAACGGAAGAAGGCAAGATACTTTATAAAATATCAATGCATTACAATTTTTCGAACACTTGTGGTTTCAACACGTGCTTTTTGTTCCTATTAGTACTGCGACGTGTACGCtatctatc      <  1:566320/251‑1 (MQ=255)

                                                                                                                                                                                                                     aGAAAAATTTGAATCCTCTCGTTTACGGGGGAGCCGGACGAAACCCTGAGAGGGCAAAATCTCGATTTTTAAGCATGAGTTTGTTAAAAAATAGAGTATATTCCCCGCCCTTCGGGCACCTTCCCCCGTAAACGGAAGAAGGCAAGATACTTTATAAAATATCAATGCATTACAATTTTTCGAACACTTGTGGTTTCAACACGTGCTTTTTGTTCCTATTAGTACTGCGACGTGTACGCTATCTATCAAGt  >  1:584865/1‑251 (MQ=255)

                                                                                                                                                                                                                       aaaaaTTTGAATCCTCTCGTTTACGGGGGAGCCGGACGAAACCCTGAGAGGGCAAAATCTCGATTTTTAAGCATGAGTTTGTTAAAAAATAGAGTATATTCCCCGCCCTTCGGGCACCTTCCCCCGTAAACGGAAGAAGGCAAGATACTTTATAAAATATCAATGCATTACAATTTTTCGAACACTTGTGGTTTCAACACGTGCTTTTTGTTCCTATTAGTACTGCGACGTGTACGCTATCTATCAAGt  <  1:355369/249‑1 (MQ=255)

                                                                                                                                                                                                                       aaaaaTTTGAATCCTCTCGTTTACGGGGGAGCCGGACGAAACCCTGAGAGGGCAAAATCTCGATTTTTAAGCATGAGTTTGTTAAAAAATAGAGTATATTCCCCGCCCTTCGGGCACCTTCCCCCGTAAACGGAAGAAGGCAAGATACTTTATAAAATATCAATGCATTACAATTTTTCGAACACTTGTGGTTTCAACACGTGCTTTTTGTTCCTATTAGTACTGCGACGTGTACGCTATCTATCAAGt  <  1:488809/249‑1 (MQ=255)

                                                                                                                                                                                                                       aaaaaTTTGAATCCTCTCGTTTACGGGGGAGCCGGACGAAACCCTGAGAGGGCAAAATCTCGATTTTTAAGCATGAGTTTGTTAAAAAATAGAGTATATTCCCCGCCCTTCGGGCACCTTCCCCCGTAAACGGAAGAAGGCAAGATACTTTATAAAATATCAATGCATTACAATTTTTCGAACACTTGTGGTTTCAACACGTGCTTTTTGTTCCTATTAGTACTGCGACGTGTACGCTATCTATCAAGt  <  1:578849/249‑1 (MQ=255)

                                                                                                                                                                                                                           aTTTGAATCCTCTCGTTTACGGGGGAGCCGGACGAAACCCTGAGAGGGCAAAATCTCGATTTTTAAGCATGAGTTTGTTAAAAAAt                                                                                                                                                                 >  1:37655/1‑86 (MQ=255)

                                                                                                                                                                                                                           aTTTGAATCCTCTCGTTTACGGGGGAGCCGGACGAAACCCTGAGAGGGCAAAATCTCGATTTTTAAGCATGAGTTTGTTAAAAAATAGAGTATATTCCCCGCCCTTCGGGCACCTTcc                                                                                                                                 <  1:778157/118‑1 (MQ=255)

                                                                                                                                                                                                                                 aTCCTCTCGTTTACGGGGGAGCCGGACGAAACCCTGAGAGGGCAAAATCTCGATTTTTAAGCATGAGTTTGTTAAAAAATAGAGTATATTcc                                                                                                                                                     <  1:589830/92‑1 (MQ=255)

                                                                                                                                                                                                                                 aTCCTCTCGTTTACGGGGGAGCCGGACGAAACCCTGAGAGGGCAAAATCTCGATTTTTAAGCATGAGTTTGTTAAAAAATAGAGTATATTCCCCGCCCTTCGGGCACCTTCCCCCGTAAACGGAAGAAGGCAAGATACTTTATAAAATATCAATGCATTACAATTTTTCGAACACTTGTGGTTTCAACACGTGCTTTTTGTTCCTATTAGTACTGCGACGTGTACGCTATCTATCAAg   >  1:397523/1‑238 (MQ=255)

                                                                                                                                                                                                                                      ctcGTTTACGGGGGAGCCGGACGAAACCCTGAGAGGGCAAAATCTCGATTTTTAAGCATGAGTTTGTTAAAAAATAGAGTATATTCCCCGCCCTTCGGGCACCTTCCCCCGTAAACGGAAGAAGGCAAGATACTTTATAAAATATCAATGCATTACAATTTTTCGAACACTTGTGGTTTCAACACGTGCTTTTTGTTCCTATTAGTACTGCGACGTGTACGCTATCTATCAAGt  <  1:552102/234‑1 (MQ=255)

                                                                                                                                                                                                                                        cGTTTACGGGGGAGCCGGACGAAACCCTGAGAGGGCAAAATCTCGATTTTTAAGCATGAGTTTGTTAAAAAATAGAGTATATTCCCCGCCCTTCGGGCACCTTCCCCCGTAAACGGAAGAAGGCAAGATACTTTATAAAATATCAATGCATTACAATTTTTCGAACACTTGTGGTTTCAACACGTGCTTTTTGTTCCTATTAGTAc                            >  1:829806/1‑206 (MQ=255)

                                                                                                                                                                                                                                        cGTTTACGGGGGAGCCGGACGAAACCCTGAGAGGGCAAAATCTCGATTTTTAAGCATGAGTTTGTTAAAAAATAGAGTATATTCCCCGCCCTTCGGGCACCTTCCCCCGTAAACGGAAGAAGGCAAGATACTTTATAAAATATCAATGCATTACAATTTTTCGAACACTTGTGGTTTCAACACGTGCTTTTTGTTCCTATTAGTACTGCGACGTGTACGCTATCTATCaa    >  1:333797/1‑230 (MQ=255)

                                                                                                                                                                                                                                          tttACGGGGGAGCCGGACGAAACCCTGAGAGGGCAAAATCTCGATTTTTAAGCATGAGTTTGTTAAAAAATAGAGTATATTCCCCGCCCTTCGGGCACCTTCCCCCGTAAACGGAAGAAGGCAAGATACTTTATAAAATATCAATGCATTACAATTTTTCGAACACTTGTGGTTTCAACACGTGCTTTTTGTTCCTATTAGTACTGCGa                       >  1:769081/1‑209 (MQ=255)

GTTATCTCCCGCCTGAAAGAATTCGGCGTCACCACCGTAGAAGAACTGCAAGGCTTGGAAGAAAATATGTTCTTTGAAGTTCCGAAAGAGTTGCGGCTGAAAGAAGCAAATTGATTTTTCACCGAATGTATTTCCGAGAATTTGATGGTGCTCATCTGATGGCGCGCATTTCCCAATTATCGCTGTCGAAACATTGTAGTTATTTAATAAAAAAGAAAAATTTGAATCCTCTCGTTTACGGGGGAACCGGACGAAACCCTGAGAGGGCAAAATCTCGATTTTTAAGCATGAGTTTGTTAAAAAATAGAGTATATTCCCCGCCCTTCGGGCACCTTCCCCCGTAAACGGAAGAAGGCAAGATACTTTATAAAATATCAATGCATTACAATTTTTCGAACACTTGTGGTTTCAACACGTGCTTTTTGTTCCTATTAGTACTGCGACGTGTACGCTATCTATCAAGT  >  1/2072010‑2072473

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`