Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	2,054,639	A→G	I237V (ATT→GTT)	unknown →	Permease of the drug/metabolite transporter (DMT) superfamily

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	2,054,639	0	A	G	100.0%	203.6 / NA	69	I237V (ATT→GTT)	unknown	Permease of the drug/metabolite transporter (DMT) superfamily
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (28/41);  total (28/41)

ATTTTAGCCAATCTGATGCTGGTTATTGCGCGCAACCGCTTACAACAATTTATTGCATTATTGATTTTATGTATGGTGGCATTCCTTGCGCTAAACGCCATTTTCGTCGCGACAGCTTTATTCCATTTATACCAACAAAGTGCGGTCGTTTTTGCCAATGAATTTGCGTTGCTGCTCTACTTTGCTTTGCATGTTGTTATGGCGTTAATTCTTGGGCTGCACATTTTCAAAAAATGGACACTATCCTATAACACGCTGTTTATTTTATTTCTGATTGCCGCCAGTTTGCCGCTCTGGGTAAATTTTGCTTATGTGGCATAAGCACATATAAATAAAGGGTTATTTTTTATTTATCAGTAAATTAAATAAAATGGAGTGAGTGAAATAATATGAATGAAAAATAATTTCGGATAAAAATATTTCATAAAAAAAATCCCCTCTCAA  >  1/2054417‑2054860                                                                                                                                                                                                                               |                                                                                                                                                                                                                              aTTTTAGCCAATCTGATGCTGGTTATTGCGCGCAACCGCTTACAACAATTTATTGCATTATTGATTTTATGTATGGTGGCATTCCTTGCGCTAAACGCCATTTTCGTCGCGACAGCTTTATTCCATTTATACCAACAAAGTGCGGTCGTTTTTGCCAATGAATTTGCGTTGCTGCTCTACTTTGCTTTGCATGTTGTTATGGCGTTAATTCTTGGGCTGCACGTTTTCAAAAAATGGACACTATCCtat                                                                                                                                                                                                     >  1:785046/1‑249 (MQ=255) aTTTTAGCCAATCTGATGCTGGTTATTGCGCGCAACCGCTTACAACAATTTATTGCATTATTGATTTTATGTATGGTGGCATTCCTTGCGCTAAACGCCATTTTCGTCGCGACAGCTTTATTCCATTTATACCAACAAAGTGCGGTCGTTTTTGCCAATGAATTTGCGTTGCTGCTCTACTTTGCTTTGCATGTTGTTATGGCGTTAATTCTTGGGCTGCACGTTTTCAAAAAATGGACACTATCCTATaa                                                                                                                                                                                                   <  1:91107/251‑1 (MQ=255) aTTTTAGCCAATCTGATGCTGGTTATTGCGCGCAACCGCTTACAACAATTTATTGCATTATTGATTTTATGTATGGTGGCATTCCTTGCGCTAAACGCCATTTTCGTCGCGACAGCTTTATTCCATTTATACCAACAAAGTGCGGTCGTTTTTGCCAATGAATTTGCGTTGCTGCTCTACTTTGCTTTGCATGTTGTTATGGCGTTAATTCTTGGGCTGCACGTTTTCAAAAAATGGACACTATCCTATaa                                                                                                                                                                                                   <  1:896380/251‑1 (MQ=255) aTTTTAGCCAATCTGATGCTGGTTATTGCGCGCAACCGCTTACAACAATTTATTGCATTATTGATTTTATGTATGGTGGCATTCCTTGCGCTAAACGCCATTTTCGTCGCGACAGCTTTATTCCATTTATACCAACAAAGTGCGGTCGTTTTTGCCAATGAATTTGCGTTGCTGCTCTACTTTGCTTTGCATGTTGTTATGGCGTTAATTCTTGGGCTGCACGTTTTCAAAAAATGGACACTATCCTATaa                                                                                                                                                                                                   >  1:822681/1‑251 (MQ=255) aTTTTAGCCAATCTGATGCTGGTTATTGCGCGCAACCGCTTACAACAATTTATTGCATTATTGATTTTATGTATGGTGGCATTCCTTGCGCTAAACGCCATTTTCGTCGCGACAGCTTTATTCCATTTATACCAACAAAGTGCGGTCGTTTTTGCCAATGAATTTGCGTTGCTGCTCTACTTTGCTTTGCATGTTGTTATGGCGTTAATTCTTGGGCTGCACGTTTTCAAAAAATGGACACTATCCTATaa                                                                                                                                                                                                   >  1:812786/1‑251 (MQ=255) aTTTTAGCCAATCTGATGCTGGTTATTGCGCGCAACCGCTTACAACAATTTATTGCATTATTGATTTTATGTATGGTGGCATTCCTTGCGCTAAACGCCATTTTCGTCGCGACAGCTTTATTCCATTTATACCAACAAAGTGCGGTCGTTTTTGCCAATGAATTTGCGTTGATGCTCTACTTTGCTTTGCATGTTGTTATGGCGTTAATTCTTGGGGTGCACGTTTTCAAAAAATGGACACTATCCTATaa                                                                                                                                                                                                   <  1:533076/251‑1 (MQ=255)   tttAGCCAATCTGATGCTGGTTATTGCGCGCAACCGCTTACAACAATTTATTGCATTATTGATTTTATGTATGGTGGCATTCCTTGCGCTAAACGCCATTTTCGTCGCGACAGCTTTATTCCATTTATACCAACAAAGTGCGGTCGTTTTTGCCAATGAATTTGCGTTGCTGCTCTACTTTGCTTTGCATGTTGTTATGGCGTTAATTCTTGGGCTGCACGTTTTCAAAAAATGGACACTATCCTATAaca                                                                                                                                                                                                 <  1:73094/251‑1 (MQ=255)    ttAGCCAATCTGATGCTGGTTATTGCGCGCAACCGCTTACAACAATTTATTGCATTATTGATTTTATGTATGGTGGCATTCCTTGCGCTAAACGCCATTTTCGTCGCGACAGCTTTATTCCATTTATACCAACAAAGTGCGGTCGTTTTTGCCAATGAATTTGCGTTGCTGCTCTACTTTGCTTTGCATGTTGTTATGGCGTTAATTCTTGGGCTGCACGTTTTCAAAAAATGGACACTATCCTATAacac                                                                                                                                                                                                <  1:282982/251‑1 (MQ=255)    ttAGCCAATCTGATGCTGGTTATTGCGCGCAACCGCTTACAACAATTTATTGCATTATTGATTTTATGTATGGTGGCATTCCTTGCGCTAAACGCCATTTTCGTCGCGACAGCTTTATTCCATTTATACCAACAAAGTGCGGTCGTTTTTGCCAATGAATTTGCGTTGCTGCTCTACTTTGCTTTGCATGTTGTTATGGCGTTAATTCTTGGGCTGCACGTTTTCAAAAAATGGACACTATCCTATAacac                                                                                                                                                                                                <  1:43988/251‑1 (MQ=255)        ccAATCTGATGCTGGTTATTGCGCGCAACCGCTTACAACAATTTATTGCATTATTGATTTTATGTATGGTGGCATTCCTTGCGCTAAACGCCATTTTCGTCGCGACAGCTTTATTCCATTTATACCAACAAAGTGCGGTCGTTTTTGCCAATGAATTTGCGTTGCTGCTCTACTTTGCTTTGCATGTTGTTATGGCGTTAATTCTTGGGCTGCACGTTTTCAAAAAATGGACACTATCCTATAACACGc                                                                                                                                                                                              <  1:369384/249‑1 (MQ=255)        ccAATCTGATGCTGGTTATTGCGCGCAACCGCTTACAACAATTTATTGCATTATTGATTTTATGTATGGTGGCATTCCTTGCGCTAAACGCCATTTTCGTCGCGACAGCTTTATTCCATTTATACCAACAAAGTGCGGTCGTTTTTGCCAATGAATTTGCGTTGCTGCTCTACTTTGCTTTGCATGTTGTTATGGCGTTAATTCTTGGGCTGCACGTTTTCAAAAAATGGACACTATCCTATAACACGCTg                                                                                                                                                                                            <  1:368393/251‑1 (MQ=255)           aTCTGATGCTGGTTATTGCGCGCAACCGCTTACAACAATTTATTGCATTATTGATTTTATGTATGGTGGCATTCCTTGCGCTAAACGCCATTTTCGTCGCGACAGCTTTATTCCATTTATACCAACAAAGTGCGGTCGTTTTTGCCAATGAATTTGCGTTGCTGCTCTACTTTGCTTTGCATGTTGTTATGGCGTTAATTCTTGGGCTGCACGTTTTCAAAAAATGGACACTATCCTATAACACGCTGttt                                                                                                                                                                                         >  1:187148/1‑251 (MQ=255)              tGATGCTGGTTATTGCGCGCAACCGCTTACAACAATTTATTGCATTATTGATTTTATGTATGGTGGCATTCCTTGCGCTAAACGCCATTTTCGTCGCGACAGCTTTATTCCATTTATACCAACAAAGTGCGGTCGTTTTTGCCAATGAATTTGCGTTGCTGCTCTACTTTGCTTTGCATGTTGTTATGGCGTTAATTCTTGGGCTGCACGTTTTCAAAAAATGGACACTATCCTATAACACGCTGtttatt                                                                                                                                                                                      <  1:767546/251‑1 (MQ=255)                aTGCTGGTTATTGCGCGCAACCGCTTACAACAATTTATTGCATTATTGATTTTATGTATGGTGGCATTCCTTGCGCTAAACGCCATTTTCGTCGCGACAGCTTTATTCCATTTATACCAACAAAGTGCGGTCGTTTTTGCCAATGAATTTGCGTTGCTGCTCTACTTTGCTTTGCATGTTGTTATGGCGTTAATTCTTGGGCTGCACGTTTTCAAAAAATGGACACTATCCTATAACACGCTGtttattt                                                                                                                                                                                     <  1:210645/250‑1 (MQ=255)                  gCTGGTTATTGCGCGCAACCGCTTACAACAATTTATTGCATTATTGATTTTATGTATGGTGGCATTCCTTGCGCTAAACGCCATTTTCGTCGCGACAGCTTTATTCCATTTATACCAACAAAGTGCGGTCGTTTTTGCCAATGAATTTGCGTTGCTGCTCTACTTTGCTTTGCATGTTGTTATGGCGTTAATTCTTGGGCTGCACGTTTTCAAAAAATGGACACTATCCTATAACACGCTGtttattttat                                                                                                                                                                                  >  1:465948/1‑251 (MQ=255)                                           caacaaTTTATTGCATTATTGATTTTATGTATGGTGGCATTCCTTGCGCTAAACGCCATTTTCGTCGCGACAGCTTTATTCCATTTATACCAACAAAGTGCGGTCGTTTTTGCCAATGAATTTGCGTTGCTGCTCTACTTTGCTTTGCATGTTGTTATGGCGTTAATTCTTGGGCTGCACGTTTTCAAAAAATGGACACTATCCTATaa                                                                                                                                                                                                   >  1:199883/1‑209 (MQ=255)                                                       gCATTATTGATTTTATGTATGGTGGCATTCCTTGCGCTAAACGCCATTTTCGTCGCGACAGCTTTATTCCATTTATACCAACAAAGTGCGGTCGTTTTTGCCAATGAATTTGCGTTGCTGCTCTACTTTGCTTTGCATGTTGTTATGGCGTTAATTCTTGGGCTGCACGTTTTCAAAAAATGGACACTATCCTATaa                                                                                                                                                                                                   <  1:781765/197‑1 (MQ=255)                                                       gCATTATTGATTTTATGTATGGTGGCATTCCTTGCGCTAAACGCCATTTTCGTCGCGACAGCTTTATTCCATTTATACCAACAAAGTGCGGTCGTTTTTGCCAATGAATTTGCGTTGCTGCTCTACTTTGCTTTGCATGTTGTTATGGCGTTAATTCTTGGGCTGCACGTTTTCAAAAAATGGACACTATCCTATaa                                                                                                                                                                                                   <  1:467648/197‑1 (MQ=255)                                                       gCATTATTGATTTTATGTATGGTGGCATTCCTTGCGCTAAACGCCATTTTCGTCGCGACAGCTTTATTCCATTTATACCAACAAAGTGCGGTCGTTTTTGCCAATGAATTTGCGTTGCTGCTCTACTTTGCTTTGCATGTTGTTATGGCGTTAATTCTTGGGCTGCACGTTTTCAAAAAATGGACACTATCCTATaa                                                                                                                                                                                                   >  1:346773/1‑197 (MQ=255)                                                       gCATTATTGATTTTATGTATGGTGGCATTCCTTGCGCTAAACGCCATTTTCGTCGCGACAGCTTTATTCCATTTATACCAACAAAGTGCGGTCGTTTTTGCCAATGAATTTGCGTTGCTGCTCTACTTTGCTTTGCATGTTGTTATGGCGTTAATTCTTGGGCTGCACGTTTTCAAAAAATGGACACTATCCTATAACACGCTGtttatttt                                                                                                                                                                                    <  1:427121/212‑1 (MQ=255)                                                       gCATTATTGATTTTATGTATGGTGGCATTCCTTGCGCTAAACGCCATTTTCGTCGCGACAGCTTTATTCCATTTATACCAACAAAGTGCGGTCGTTTTTGCCAATGAATTTGCGTTGCTGCTCTACTTTGCTTTGCATGTTGTTATGGCGTTAATTCTTGGGCTGCACGTTTTCAAAAAATGGACACTATCCTATAACACGCTGTTTATTTTATTTCTGATTGCCGCCAGTTTGCCGCTCTGGGTAAAttt                                                                                                                                             >  1:279584/1‑251 (MQ=255)                                                                aTTTTATGTATGGTGGCATTCCTTGCGCTAAACGCCATTTTCGTCGCGACAGCTTTATTCCATTTATACCAACAAAGTGCGGTCGTTTTTGCCAATGAATTTGCGTTGCTGCTCTACTTTGCTTTGCATGTTGTTATGGCGTTAATTCTTGGGCTGCACGTTTTCAAAAAATGGACACTATCCtat                                                                                                                                                                                                     <  1:82349/186‑1 (MQ=255)                                                                aTTTTATGTATGGTGGCATTCCTTGCGCTAAACGCCATTTTCGTCGCGACAGCTTTATTCCATTTATACCAACAAAGTGCGGTCGTTTTTGCCAATGAATTTGCGTTGCTGCTCTACTTTGCTTTGCATGTTGTTATGGCGTTAATTCTTGGGCTGCACGTTTTCAAAAAATGGACACTATCCTATaa                                                                                                                                                                                                   >  1:793455/1‑188 (MQ=255)                                                                    tatgtatgGTGGCATTCCTTGCGCTAAACGCCATTTTCGTCGCGACAGCTTTATTCCATTTATACCAACAAAGTGCGGTCGTTTTTGCCAATGAATTTGCGTTGCTGCTCTACTTTGCTTTGCATGTTGTTATGGCGTTAATTCTTGGGCTGCACGTTTTCAAAAAATGGACACTATCCTATaa                                                                                                                                                                                                   >  1:670081/1‑184 (MQ=255)                                                                     atgtatgGTGGCATTCCTTGCGCTAAACGCCATTTTCGTCGCGACAGCTTTATTCCATTTATACCAACAAAGTGCGGTCGTTTTTGCCAATGAATTTGCGTTGCTGCTCTACTTTGCTTTGCATGTTGTTATGGCGTTAATTCTTGGGCTGCACGTTTTCAAAAAATGGACACTATCCTATaa                                                                                                                                                                                                   <  1:833865/183‑1 (MQ=255)                                                                     atgtatgGTGGCATTCCTTGCGCTAAACGCCATTTTCGTCGCGACAGCTTTATTCCATTTATACCAACAAAGTGCGGTCGTTTTTGCCAATGAATTTGCGTTGCTGCTCTACTTTGCTTTGCATGTTGTTATGGCGTTAATTCTTGGGCTGCACGTTTTCAAAAAATGGACACTATCCTATAACACGCTGTTTATTTTATTTCTGATTGCCGCCAGTTTGCCGCTCTGGGTAAAttt                                                                                                                                             >  1:239805/1‑237 (MQ=255)                                                                         atgGTGGCATTCCTTGCGCTAAACGCCATTTTCGTCGCGACAGCTTTATTCCATTTATACCAACAAAGTGCGGTCGTTTTTGCCAATGAATTTGCGTTGCTGCTCTACTTTGCTTTGCATGTTGTTATGGCGTTAATTCTTGGGCTGCACGTTTTCAAAAAATGGacac                                                                                                                                                                                                             >  1:63247/1‑169 (MQ=255)                                                                         atgGTGGCATTCCTTGCGCTAAACGCCATTTTCGTCGCGACAGCTTTATTCCATTTATACCAACAAAGTGCGGTCGTTTTTGCCAATGAATTTGCGTTGCTGCTCTACTTTGCTTTGCATGTTGTTATGGCGTTAATTCTTGGGCTGCACGTTTTCAAAAAATGGACACTATCCTATAac                                                                                                                                                                                                  >  1:402259/1‑180 (MQ=255)                                                                         atgGTGGCATTCCTTGCGCTAAACGCCATTTTCGTCGCGACAGCTTTATTCCATTTATACCAACAAAGTGCGGTCGTTTTTGCCAATGAATTTGCGTTGCTGCTCTACTTTGCTTTGCATGTTGTTATGGCGTTAATTCTTGGGCTGCACGTTTTCAAAAAATGGACACTATCCTATAACACGCTGTTTATTTTATTTCTGATTGCCGCCAGTTTGCCGCTCTGGGTAAATTTTGCTTATGTGGCATAAGc                                                                                                                           <  1:818373/251‑1 (MQ=255)                                                                          tggtggCATTCCTTGCGCTAAACGCCATTTTCGTCGCGACAGCTTTATTCCATTTATACCAACAAAGTGCGGTCGTTTTTGCCAATGAATTTGCGTTGCTGCTCTACTTTGCTTTGCATGTTGTTATGGCGTTAATTCTTGGGCTGCACGTTTTCAAAAAATGGACACTATCCTATAACACGCTGTTTATTTTATTTCTGATTGCCGCCAGTTTGCCGCTCTGGGTAAATTTTGCTTATGTGGCATAAGca                                                                                                                          <  1:63817/251‑1 (MQ=255)                                                                              ggCATTCCTTGCGCTAAACGCCATTTTCGTCGCGACAGCTTTATTCCATTTATACCAACAAAGTGCGGTCGTTTTTGCCAATGAATTTGCGTTGCTGCTCTACTTTGCTTTGCATGTTGTTATGGCGTTAATTCTTGGGCTGCACGTTTTCAAAAAATGGACACTATCCTATAACACGCTGTTTATTTTATTTCTGATTGCCGCCAGTTTGCCGCTCTGGGTAAATTTTGCTTATGTGGCATAAGc                                                                                                                           >  1:95140/1‑246 (MQ=255)                                                                                cATTCCTTGCGCTAAACGCCATTTTCGTCGCGACAGCTTTATTCCATTTATACCAACAAAGTGCGGTCGTTTTTGCCAATGAATTTGCGTTGCTGCTCTACTTTGCTTTGCATGTTGTTATGGCGTTAATTCTTGGGCTGCACGTTTTCAAAAAATGGACACTATCCTATAACACGCTGTTTATTTTATTTCTGATTGCCGCCAGTTTGCCGCTCTGGGTAAATTTTGCTTATGTGGCATAAGCACatat                                                                                                                     <  1:331977/250‑1 (MQ=255)                                                                                cATTCCTTGCGCTAAACGCCATTTTCGTCGCGACAGCTTTATTCCATTTATACCAACAAAGTGCGGTCGTTTTTGCCAATGAATTTGCGTTGCTGCTCTACTTTGCTTTGCATGTTGTTATGGCGTTAATTCTTGGGCTGCACGTTTTCAAAAAATGGACACTATCCTATAACACGCTGTTTATTTTATTTCTGATTGCCGCCAGTTTGCCGCTCTGGGTAAATTTTGCTTATGTGGCATAAGCACatat                                                                                                                     <  1:277388/250‑1 (MQ=255)                                                                                cATTCCTTGCGCTAAACGCCATTTTCGTCGCGACAGCTTTATTCCATTTATACCAACAAAGTGCGGTCGTTTTTGCCAATGAATTTGCGTTGCTGCTCTACTTTGCTTTGCATGTTGTTATGGCGTTAATTCTTGGGCTGCACGTTTTCAAAAAATGGACACTATCCTATAACACGCTGTTTATTTTATTTCTGATTGCCGCCAGTTTGCCGCTCTGGGTAAATTTTGCTTATGTGGCATAAGCACAtata                                                                                                                    <  1:762408/251‑1 (MQ=255)                                                                                cATTCCTTGCGCTAAACGCCATTTTCGTCGCGACAGCTTTATTCCATTTATACCAACAAAGTGCGGTCGTTTTTGCCAATGAATTTGCGTTGCTGCTCTACTTTGCTTTGCATGTTGTTATGGCGTTAATTCTTGGGCTGCACGTTTTCAAAAAATGGACACTATCCTATAACACGCTGTTTATTTTATTTCTGATTGCCGCCAGTTTGCCGCTCTGGGTAAATTTTGCTTATGTGGCATAAGCACAtata                                                                                                                    <  1:1374/251‑1 (MQ=255)                                                                                   tCCTTGCGCTAAACGCCATTTTCGTCGCGACAGCTTTATTCCATTTATACCAACAAAGTGCGGTCGTTTTTGTCAATGAATTTGCGTTGCTGCTCTACTTTGCTTTGCATGTTGTTATGGCGTTAATTCTTGGGCTGCACGTTTTCAAAAAATGGACACTATCCTATAACACGCTGTTTATTTTATTTCTGATTGCCGCCAGTTTGCCGCTCTGGGTAAATTTTGCTTATGTGGCATAAGca                                                                                                                          <  1:456223/242‑1 (MQ=255)                                                                                   tCCTTGCGCTAAACGCCATTTTCGTCGCGACAGCTTTATTCCATTTATACCAACAAAGTGCGGTCGTTTTTGCCAATGAATTTGCGTTGCTGCTCTACTTTGCTTTGCATGTTGTTATGGCGTTAATTCTTGGGCTGCACGTTTTCAAAAAATGGACACTATCCTATaa                                                                                                                                                                                                   <  1:618198/169‑1 (MQ=255)                                                                                   tCCTTGCGCTAAACGCCATTTTCGTCGCGACAGCTTTATTCCATTTATACCAACAAAGTGCGGTCGTTTTTGCCAATGAATTTGCGTTGCTGCTCTACTTTGCTTTGCATGTTGTTATGGCGTTAATTCTTGGGCTGCACGTTTTCAAAAAATGGACACTATCCTATAacac                                                                                                                                                                                                >  1:111948/1‑172 (MQ=255)                                                                                   tCCTTGCGCTAAACGCCATTTTCGTCGCGACAGCTTTATTCCATTTATACCAACAAAGTGCGGTCGTTTTTGCCAATGAATTTGCGTTGCTGCTCTACTTTGCTTTGCATGTTGTTATGGCGTTAATTCTTGGGCTGCACGTTTTCAAAAAATGGACACTATCCTATAACACGCTGTTTATTTTATTTCTGATTGCCGCCAGTTTGCCGCTCTGGGTAAATTTTGCTTAtgtg                                                                                                                                   >  1:125461/1‑233 (MQ=255)                                                                                   tCCTTGCGCTAAACGCCATTTTCGTCGCGACAGCTTTATTCCATTTATACCAACAAAGTGCGGTCGTTTTTGCCAATGAATTTGCGTTGCTGCTCTACTTTGCTTTGCATGTTGTTATGGCGTTAATTCTTGGGCTGCACGTTTTCAAAAAATGGACACTATCCTATAACACGCTGTTTATTTTATTTCTGATTGCCGCCAGTTTGCCGCTCTGGGTAAATTTTGCTTATGTGGCATAAGCACATataaat                                                                                                                 <  1:504271/251‑1 (MQ=255)                                                                                   tCCTTGCGCTAAACGCCATTTTCGTCGCGACAGCTTTATTCCATTTATACCAACAAAGTGCGGTCGTTTTTGCCAATGAATTTGCGTTGCTGCTCTACTTTGCTTTGCATGTTGTTATGGCGTTAATTCTTGGGCTGCACGTTTTCAAAAAATGGACACTATCCTATAACACGCTGTTTATTTTATTTCTGATTGCCGCCAGTTTGCCGCTCTGGGTAAATTTTGCTTATGTGGCATAAGCACATataaat                                                                                                                 <  1:178268/251‑1 (MQ=255)                                                                                   tCCTTGCGCTAAACGCCATTTTCGTCGCGACAGCTTTATTCCATTTATACCAACAAAGTGCGGTCGTTTTTGCCAATGAATTTGCGTTGCTGCTCTACTTTGCTTTGCATGTTGTTATGGCGTTAATTCTTGGGCTGCACGTTTTCAAAAAATGGACACTATCCTATAACACGCTGTTTATTTTATTTCTGATTGCCGCCAGTTTGCCGCTCTGGGTAAATTTTGCTTATGTGGCATAAGCACATataaat                                                                                                                 <  1:245812/251‑1 (MQ=255)                                                                                   tCCTTGCGCTAAACGCCATTTTCGTCGCGACAGCTTTATTCCATTTATACCAACAAAGTGCGGTCGTTTTTGCCAATGAATTTGCGTTGCTGCTCTACTTTGCTTTGCATGTTGTTATGGCGTTAATTCTTGGGCTGCACGTTTTCAAAAAATGGACACTATCCTATAACACGCTGTTTATTTTATTTCTGATTGCCGCCAGTTTGCCGCTCTGGGTAAATTTTGCTTATGTGGCATAAGCACATataaat                                                                                                                 <  1:274020/251‑1 (MQ=255)                                                                                   tCCTTGCGCTAAACGCCATTTTCGTCGCGACAGCTTTATTCCATTTATACCAACAAAGTGCGGTCGTTTTTGCCAATGAATTTGCGTTGCTGCTCTACTTTGCTTTGCATGTTGTTATGGCGTTAATTCTTGGGCTGCACGTTTTCAAAAAATGGACACTATCCTATAACACGCTGTTTATTTTATTTCTGATTGCCGCCAGTTTGCCGCTCTGGGTAAATTTTGCTTATGTGGCATAAGCACATataaat                                                                                                                 <  1:754251/251‑1 (MQ=255)                                                                                   tCCTTGCGCTAAACGCCATTTTCGTCGCGACAGCTTTATTCCATTTATACCAACAAAGTGCGGTCGTTTTTGCCAATGAATTTGCGTTGCTGCTCTACTTTGCTTTGCATGTTGTTATGGCGTTAATTCTTGGGCTGCACGTTTTCAAAAAATGGACACTATCCTATAACACGCTGTTTATTTTATTTCTGATTGCCGCCAGTTTGCCGCTCTGGGTAAATTTTGCTTATGTGGCATAAGCACATataaat                                                                                                                 <  1:718658/251‑1 (MQ=255)                                                                                   tCCTTGCGCTAAACGCCATTTTCGTCGCGACAGCTTTATTCCATTTATACCAACAAAGTGCGGTCGTTTTTGCCAATGAATTTGCGTTGCTGCTCTACTTTGCTTTGCATGTTGTTATGGCGTTAATTCTTGGGCTGCACGTTTTCAAAAAATGGACACTATCCTATAACACGCTGTTTATTTTATTTCTGATTGCCGCCAGTTTGCCGCTCTGGGTAAATTTTGCTTATGTGGCATAAGCACATataaat                                                                                                                 <  1:651978/251‑1 (MQ=255)                                                                                   tCCTTGCGCTAAACGCCATTTTCGTCGCGACAGCTTTATTCCATTTATACCAACAAAGTGCGGTCGTTTTTGCCAATGAATTTGCGTTGCTGCTCTACTTTGCTTTGCATGTTGTTATGGCGTTAATTCTTGGGCTGCACGTTTTCAAAAAATGGACACTATCCTATAACACGCTGTTTATTTTATTTCTGATTGCCGCCAGTTTGCCGCTCTGGGTAAATTTTGCTTATGTGGCATAAGCACATataaat                                                                                                                 <  1:568525/251‑1 (MQ=255)                                                                                   tCCTTGCGCTAAACGCCATTTTCGTCGCGACAGCTTTATTCCATTTATACCAACAAAGTGCGGTCGTTTTTGCCAATGAATTTGCGTTGCTGCTCTACTTTGCTTTGCATGTTGTTATGGCGTTAATTCTTGGGCTGCACGTTTTCAAAAAATGGACACTATCCTATAACACGCTGTTTATTTTATTTCTGATTGCCGCCAGTTTGCCGCTCTGGGTAAATTTTGCTTATGTGGCATAAGCACATataaat                                                                                                                 <  1:591959/251‑1 (MQ=255)                                                                                    ccTTGCGCTAAACGCCATTTTCGTCGCGACAGCTTTATTCCATTTATACCAACAAAGTGCGGTCGTTTTTGCCAATGAATTTGCGTTGCTGCTCTACTTTGCTTTGCATGTTGTTATGGCGTTAATTCTTGGGCTGCACGTTTTCAAAAAATGGACACTATCCTATAACACGCTGTTTATTTTATTTCTGATTGCCGCCAGTTTGCCGCTCTGGGTAAATTTTGCTTATGTGGCATAAGCACATataaat                                                                                                                 >  1:409392/1‑250 (MQ=255)                                                                                                    aTTTTCGTCGCGACAGCTTTATTCCATTTATACCAACAAAGTGCGGTCGTTTTTGCCAATGAATTTGCGTTGCTGCTCTACTTTGCTTTGCATGTTGTTATGGCGTTAATTCTTGGGCTGCACGTTTTCAAAAAATGGACACTATCCTATAACACGCTGTTTATTTTATTTCTGATTGCCGCCAGTTTGCCGCTCTGGGTAAATTTTGCTTATGTGGCATAAGCACATATAAATAAAGGGTTATTTtttat                                                                                                <  1:264395/251‑1 (MQ=255)                                                                                                              cgACAGCTTTATTCCATTTATACCAACAAAGTGCGGTCGTTTTTGCCAATGAATTTGCGTTGCTGCTCTACTTTGCTTTGCATGTTGTTATGGCGTTAATTCTTGGGCTGCACGTTTTCAAAAAATGGACACTATCCTATAACACGCTGtttatttt                                                                                                                                                                                    >  1:679660/1‑157 (MQ=255)                                                                                                                   gCTTTATTCCATTTATACCAACAAAGTGCGGTCGTTTTTGCCAATGAATTTGCGTTGCTGCTCTACTTTGCTTTGCATGTTGTTATGGCGTTAATTCTTGGGCTGCACGTTTTCAAAAAATGGACACTATCCTATAACACGCTGTTTATTTTATTTCTGATTGCCGCCAGTTTGCCGCTCTGGGTAAA‑TTTGCTTATGTGGCATAAGCACATATAAATAAAGGGTTATTTtttat                                                                                                >  1:258342/1‑235 (MQ=255)                                                                                                                   gCTTTATTCCATTTATACCAACAAAGTGCGGTCGTTTTTGCCAATGAATTTGCGTTGCTGCTCTACTTTGCTTTGCATGTTGTTATGGCGTTAATTCTTGGGCTGCACGTTTTCAAAAAATGGACACTATCCTATAACACGCTGTTTATTTTATTTCTGA‑TGCCGCCAGTTTGCCGCTCTGGGTAAATTTTGCTTATGTGGCATAAGCACATataaataa                                                                                                               <  1:20363/220‑1 (MQ=255)                                                                                                                    cTTTATTCCATTTATACCAACAAAGTGCGGTCGTTTTTGCCAATGAATTTGCGTTGCTGCTCTACTTTGCTTTGCATGTTGTTATGGCGTTAATTCTTGGGCTGCACGTTTTCAAAAAATGGACACTATCCTATaa                                                                                                                                                                                                   <  1:10319/136‑1 (MQ=255)                                                                                                                        aTTCCATTTATACCAACAAAGTGCGGTCGTTTTTGCCAATGAATTTGCGTTGCTGCTCTACTTTGCTTTGCATGTTGTTATGGCGTTAATTCTTGGGCTGCACGTTTTCAAAAAATGGACACTATCCTATAACACGCTGTTTATTTTATTTCTGATTGCCGCCAGTTTGCCGCTCTGGGTAAATTTTGCTTATGTGGCATAAGCACATATAAATAAAGGGTTATTTTTTATTTATCAGTAAATtaaataaa                                                                            >  1:124077/1‑251 (MQ=255)                                                                                                                             aTTTATACCAACAAAGTGCGGTCGTTTTTGCCAATGAATTTGCGTTGCTGCTCTACTTTGCTTTGCATGTTGTTATGGCGTTAATTCTTGGGCTGCACGTTTTCAAAAAATGGACACTATCCTATaa                                                                                                                                                                                                   >  1:721702/1‑127 (MQ=255)                                                                                                                             aTTTATACCAACAAAGTGCGGTCGTTTTTGCCAATGAATTTGCGTTGCTGCTCTACTTTGCTTTGCATGTTGTTATGGCGTTAATTCTTGGGCTGCACGTTTTCAAAAAATGGACACTATCCTATAACACGCTGTTTATTTTATTTCTGATTGCCGCCAGTTTGCCGCTCTGGGTAAATTTTGCTTATGTGGCATAAGCACATATAAATAAAGGGTTATTTTTTATTTATCAGTAAATTAAATAAAATGga                                                                       >  1:812497/1‑251 (MQ=255)                                                                                                                              tttATACCAACAAAGTGCGGTCGTTTTTGCCAATGAATTTGCGTTGCTGCTCTACTTTGCTTTGCATGTTGTTATGGCGTTAATTCTTGGGCTGCACGTTTTCAAAAAATGGACACTATCCTATAACACGCTGTTTATTTTATTTCTGATTGCCGCCAGTTTGCCGCTCTGGGTAAATTTTGCTTATGTGGCATAAGCACATATAAATAAAGGGTTATTTTTTATTTATCAGTAAATTAAATAAAATGgag                                                                      <  1:684620/251‑1 (MQ=255)                                                                                                                                      aacaaAGTGCGGTCGTTTTTGCCAATGAATTTGCGTTGCTGCTCTACTTTGCTTTGCATGTTGTTATGGCGTTAATTCTTGGGCTGCACGTTTTCAAAAAATGGACACTATCCTATaa                                                                                                                                                                                                   >  1:611610/1‑118 (MQ=255)                                                                                                                                             tGCGGTCGTTTTTGCCAATGAATTTGCGTTGCTGCTCTACTTTGCTTTGCATGTTGTTATGGCGTTAATTCTTGGGCTGCACGTTTTCAAAAAATGGACACTATCCTATAACACGCTGTTTATTTTATTTCTGATTGCCGCCAGTTTGCCGCTCTGGGTAAATTTTGCTTATGTGGCATAAGCACATATAAATAAAGGGTTATTTTTTATTTATCAGTAAATTAAATAAAATGGAGTGAGTGAAataata                                                        <  1:110400/250‑1 (MQ=255)                                                                                                                                                                gAATTTGCGTTGCTGCTCTACTTTGCTTTGCATGTTGTTATGGCGTTAATCCTTGGGCTGCACGTTTTCAAAAAATGGACACTATCCTATAACACGCTGTTTATTTTATTTCTGATTGCCGCCAGTTTGCCGCTCTGGGTAAATTTTGCTTATGTGGCATAAGCACATataaataa                                                                                                               >  1:264912/1‑176 (MQ=255)                                                                                                                                                                      gCGTTGCTGCTCTACTTTGCTTTGCATGTTGTTATGGCGTTAATTCTTGGGCTGCACGTTTTCAAAAAATGGACACTATCCTATAACACGCTGTTTATTTTATTTCTGATTGCCGCCAGTTTGCCGCTCTGGGTAAATTTTGCTTATGTGGCATAAGCACATATAAATAAAg                                                                                                             >  1:180349/1‑172 (MQ=255)                                                                                                                                                                                 ctACTTTGCTTTGCATGTTGTTATGGCGTTAATTCTTGGGCTGCACGTTTTCAAAAAATGGACACTATCCTATAACACGCTGTTTATTTTATTTCTGATTGCCGCCAGTTTGCCGCTCTGGGTAAATTTTGCTTATGTGGCATAAGCACATATAAATAAAGGGTTATTTTTTATTTATCAGTAAATTAAATAAAATGGAGTGAGTGAAATAATatg                                                      <  1:389089/216‑1 (MQ=255)                                                                                                                                                                                 ctACTTTGCTTTGCATGTTGTTATGGCGTTAATTCTTGGGCTGCACGTTTTCAAAAAATGGACACTATCCTATAACACGCTGTTTATTTTATTTCTGATTGCCGCCAGTTTGCCGCTCTGGGTAAATTTTGCTTATGTGGCATAAGCACATATAAATAAAGGGTTATTTTTTATTTATCAGTAAATTAAATAAAATGGAGTGAGTGAAATAATatg                                                      <  1:306949/216‑1 (MQ=255)                                                                                                                                                                                 ctACTTTGCTTTGCATGTTGTTATGGCGTTAATTCTTGGGCTGCACGTTTTCAAAAAATGGACACTATCCTATAACACGCTGTTTATTTTATTTCTGATTGCCGCCAGTTTGCCGCTCTGGGTAAATTTTGCTTATGTGGCATAAGCACATATAAATAAAGGGTTATTTTTTATTTATCAGTAAATTAAATAAAATGGAGTGAGTGAAATAATATGAATGAAAAATAATTTCGGATAAAAATATTTCATaa                   >  1:126155/1‑251 (MQ=255)                                                                                                                                                                                        gctttgcATGTTGTTATGGCGTTAATTCTTGGGCTGCACGTTTTCAAAAAATGGACACTATCCTATAACACGCTGTTTATTTTATTTCTGATTGCCGCCAGTTTGCCGCTCTGGGTAAATTTTGCTTATGTGGCATAAGCACATataaat                                                                                                                 >  1:301669/1‑150 (MQ=255)                                                                                                                                                                                             gcATGTTGTTATGGCGTTAATTCTTGGGCTGCACGTTTTCAAAAAATGGACACTATCCTATAACACGCTGTTTATTTTATTTCTGATTGCCGCCAGTTTGCCGCTCTGGGTAAATTTTGCTTATGTGGCATAAGc                                                                                                                           <  1:422445/135‑1 (MQ=255)                                                                                                                                                                                                  ttgttATGGCGTTAATTCTTGGGCTGCACGTTTTCAAAAAATGGACACTATCCTATAacac                                                                                                                                                                                                <  1:871324/61‑1 (MQ=255)                                                                                                                                                                                                  ttgttATGGCGTTAATTCTTGGGCTGCACGTTTTCAAAAAATGGACACTATCCTATAACACGCTGTTTATTTTATTTCTGATTGCCGCCAGTTTGCCGCTCTGGGTAAATTTTGCTTATGTGGCATAAGCACATATAAATAAAGGGTTATTTTTTATTTATCAGTAAATTAAATAAAATGGAGTGAGTGAAATAATATGAATGAAAAATAATTTCGGATAAAAATATTTCATAAAAAAAATCCCCTCTCaa  >  1:849508/1‑251 (MQ=255)                                                                                                                                                                                                                               |                                                                                                                                                                                                                              ATTTTAGCCAATCTGATGCTGGTTATTGCGCGCAACCGCTTACAACAATTTATTGCATTATTGATTTTATGTATGGTGGCATTCCTTGCGCTAAACGCCATTTTCGTCGCGACAGCTTTATTCCATTTATACCAACAAAGTGCGGTCGTTTTTGCCAATGAATTTGCGTTGCTGCTCTACTTTGCTTTGCATGTTGTTATGGCGTTAATTCTTGGGCTGCACATTTTCAAAAAATGGACACTATCCTATAACACGCTGTTTATTTTATTTCTGATTGCCGCCAGTTTGCCGCTCTGGGTAAATTTTGCTTATGTGGCATAAGCACATATAAATAAAGGGTTATTTTTTATTTATCAGTAAATTAAATAAAATGGAGTGAGTGAAATAATATGAATGAAAAATAATTTCGGATAAAAATATTTCATAAAAAAAATCCCCTCTCAA  >  1/2054417‑2054860

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑