Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	2,049,717	A→G	intergenic (+552/‑55)	unknown → / → unknown	tRNA‑Cys‑GCA/hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	2,049,717	0	A	G	100.0%	216.8 / NA	71	intergenic (+552/‑55)	unknown/unknown	tRNA‑Cys‑GCA/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (42/29);  total (42/29)

AATATGGATCAATCGTTAGACTTAGTATTTATTGAATATGTTCAACCCTATTTATTCAAATTGAAAAAGTTTACTTAATAATGATGGAGAAAGTTCTTCATATTCTGATTTTAGTTTAGAGAGTTTAAACGCAGTATCCTTAAACCGGTTTTAAAAGATCCCGGTCAGTATTCTAATATGATTGTTAGCATTAAAACAAAAGGTCGTCCTGTTAGATAATTGATCTTTATATTGCCTTATTGATGGCATTATCTCTAAATACCGTCCTAACTTGTAATAACAAAAGATTGCTATTTAATGGTCCCTGAAATTTTATTAGCTTGCTCAACAATAGTTCACATTGAAACACTTCACGCATTAATTCAAACAGAAAGCTCATATAATCCTTATGCTATCGCAGTAGTAAATGATATTCCTCTTGCTCAACAGCCTAAAACACTCCAAGAAGCAGAATTAGTCATAGATGAACTTGAAGCTAAAAAAA  >  1/2049475‑2049958                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                  aaTATGGATCAATCGTTAGACTTAGTATTTATTGAATATGTTCAACCCTATTTATTCAAATTGAAAAAGTTTACTTAATAATGATGGAGAAAGTTCTTCATATTCTGATTTTAGTTTAGAGAGTTTAAACGCAGTATCCTTAAACCGGTTTTAAAAGATCCCGGTCAGTATTCTAATATGATTGTTAGCATTAAAACAAAAGGTCGTCCTGTTAGATAATTGATCTTTATATTGCCTTATTGGTGGCATTa                                                                                                                                                                                                                                           >  1:640535/1‑251 (MQ=255)            aTCGTTAGACTTAGTATTTATTGAATATGTTCAACCCTATTTATTCAAATTGAAAAAGTTTACTTAATAATGATGGAGAAAGTTCTTCATATTCTGATTTTAGTTTAGAGAGTTTAAACGCAGTATCCTTAAACCGGTTTTAAAAGATCCCGGTCAGTATTCTAATATGATTGTTAGCATTAAAACAAAAGGTCGTCCTGTTAGATAATTGATCTTTATATTGCCTTATTGGTGGCATTATCTCTAAATAc                                                                                                                                                                                                                                <  1:713978/251‑1 (MQ=255)            aTCGTTAGACTTAGTATTTATTGAATATGTTCAACCCTATTTATTCAAATTGAAAAAGTTTACTTAATAATGATGGAGAAAGTTCTTCATATTCTGATTTTAGTTTAGAGAGTTTAAACGCAGTATCCTTAAACCGGTTTTAAAAGATCCCGGTCAGTATTCTAATATGATTGTTAGCATTAAAACAAAAGGTCGTCCTGTTAGATAATTGATCTTTATATTGCCTTATTGGTGGCATTATCTCTAAATAc                                                                                                                                                                                                                                <  1:212632/251‑1 (MQ=255)                   gACTTAGTATTTATTGAATATGTTCAACCCTATTTATTCAAATTGAAAAAGTTTACTTAATAATGATGGAGAAAGTTCTTCATATTCTGATTTTAGTTTAGAGAGTTTAAACGCAGTATCCTTAAACCGGTTTTAAAAGATCCCGGTCAGTATTCTAATATGATTGTTAGCATTAAAACAAAAGGTCGTCCTGTTAGATAATTGATCTTTATATTGCCTTATTGGTGGCATTATCTCTAAATACCGTCCTa                                                                                                                                                                                                                         >  1:681829/1‑251 (MQ=255)                    aCTTAGTATTTATTGAATATGTTCAACCCTATTTATTCAAATTGAAAAAGTTTACTTAATAATGATGGAGAAAGTTCTTCATATTCTGATTTTAGTTTAGAGAGTTTAAACGCAGTATCCTTAAACCGGTTTTAAAAGATCCCGGTCAGTATTCTAATATGATTGTTAGCATTAAAACAAAAGGTCGTCCTGTTAGATAATTGATCTTTATATTGCCTTATTGGTGGCATTATCTCTAAATACCGTCCTaa                                                                                                                                                                                                                        >  1:196122/1‑251 (MQ=255)                                  gAATATGTTCAACCCTATTTATTCAAATTGAAAAAGTTTACTTAATAATGATGGAGAAAGTTCTTCATATTCTGATTTTAGTTTAGAGAGTTTAAACGCAGTATCCTTAAACCGGTTTTAAAAGATCCCGGTCAGTATTCTA‑‑ATGATTGTTAGCATTAAAACAAAAGGTCGTCCTGTTAGATAATTGATCTTTATATTGCCTTATTGGTGGCATTATCTCTAAATACCGTCCTAACTTGTAATAACAAAAg                                                                                                                                                                                                        >  1:4687/1‑251 (MQ=255)                                                 tatttattCAAATTGAAAAAGTTTACTTAATAATGATGGAGAAAGTTCTTCATATTCTGATTTTAGTTTAGAGAGTTTAAACGCAGTATCCTTAAACCGGTTTTAAAAGATCCCGGTCAGTATTCTAATATGATTGTTAGCATTAAAACAAAAGGTCGTCCTGTTAGATAATTGATCTTTATATTGCCTTATTGGTGGCATTATCTCTAAATAc                                                                                                                                                                                                                                >  1:519727/1‑214 (MQ=255)                                                             ttGAAAAAGTTTACTTAATAATGATGGAGAAAGTTCTTCATATTCTGATTTTAGTTTAGAGAGTTTAAACGCAGTATCCTTAAACCGGTTTTAAAAGATCCCGGTCAGTATTCTAATATGATTGTTAGCATTAAAACAAAAGGTCGTCCTGTTAGATAATTGATCTTTATATTGCCTTATTGGTGGCATTATCTCTAAATACCGTCCTAACTTGTAATAACAAAAGATTGCTATTTAATgg                                                                                                                                                                                         <  1:712390/241‑1 (MQ=255)                                                                     gTTTACTTAATAATGATGGAGAAAGTTCTTCATATTCTGATTTTAGTTTAGAGAGTTTAAACGCAGTATCCTTAAACCGGTTTTAAAAGATCCCGGTCAGTATTCTAATATGATTGTTAGCATTAAAACAAAAGGTCGTCCTGTTAGATAATTGATCTTTATATTGCCTTATTGGTGGCATTATCTCTAAATACCGTCCTAACTTGTAATAACAACAGATTGCTATTTAATGGTCCCTGAAATTTTATTAg                                                                                                                                                                       >  1:669752/1‑251 (MQ=255)                                                                       ttACTTAATAATGATGGAGAAAGTTCTTCATATTCTGATTTTAGTTTAGAGAGTTTAAACGCAGTATCCTTAAACCGGTTTTAAAAGATCCCGGTCAGTATTCTAATATGATTGTTAGCATTAAAACAAAAGGTCGTCCTGTTAGATAATTGATCTTTATATTGCCTTATTGGTGGCATTATCTCTAAATACCGTCCTAACTTGTAATAACAAAAGATTGCTATTTAATGGTCCCTGAAATTTTATTAGCt                                                                                                                                                                     <  1:15669/251‑1 (MQ=255)                                                                         aCTTAATAATGATGGAGAAAGTTCTTCATATTCTGATTTTAGTTTAGAGAGTTTAAACGCAGTATCCTTAAACCGGTTTTAAAAGATCCCGGTCAGTATTCTAATATGATTGTTAGCATTAAAACAAAAGGTCGTCCTGTTAGATAATTGATCTTTATATTGCCTTATTGGTGGCATTATCTCTAAATACCGTCCTAACt                                                                                                                                                                                                                      >  1:480694/1‑200 (MQ=255)                                                                          cTTAATAATGATGGAGAAAGTTCTTCATATTCTGATTTTAGTTTAGAGAGTTTAAACGCAGTATCCTTAAACCGGTTTTAAAAGATCCCGGTCAGTATTCTAATATGATTGTTAGCATTAAAACAAAAGGTCGTCCTGTTAGATAATTGATCTTTATATTGCCTTATTGGTGGCATTATCTCTAAATACCGTCCTAACTTGTAATAACAAAAGATTGCTATTTAATGGTCCCTGAAATTTTATTAGCTTg                                                                                                                                                                   >  1:76196/1‑250 (MQ=255)                                                                               taatGATGGAGAAAGTTCTTCATATTCTGATTTTAGTTTAGAGAGTTTAAACGCAGTATCCTTAAACCGGTTTTAAAAGATCCCGGTCAGTATTCTAATATGATTGTTAGCATTAAAACAAAAGGTCGTCCTGTTAGATAATTGATCTTTATATTGCCTTATTGGTGGCATTATCTCTAAATACCGTCCTAACTTGTAATAACAAAAGATTGCTAttt                                                                                                                                                                                              <  1:68277/218‑1 (MQ=255)                                                                                  tgatgGAGAAAGTTCTTCATATTCTGATTTTAGTTTAGAGAGTTTAAACGCAGTATCCTTAAACCGGTTTTAAAAGATCCCGGTCAGTATTCTAATATGATTGTTAGCATTAAAACAAAAGGTCGTCCTGTTAGATAATTGATCTTTATATTGCCTTATTGGTGGCATTATCTCTAAATACCGTCCTAACTTGTAATAACAAAAGATTGCTATTTAATGGTCCCTGAAATTTTATTAGCTTGCTCAACAAt                                                                                                                                                          <  1:50872/251‑1 (MQ=255)                                                                                   gatgGAGAAAGTTCTTCATATTCTGATTTTAGTTTAGAGAGTTTAAACGCAGTATCCTTAAACCGGTTTTAAAAGATCCCGGTCAGTATTCTAATATGATTGTTAGCATTAAAACAAAAGGTCGTCCTGTTAGATAATTGATCTTTATATTGCCTTATTGGTGGCATTATCTCTAAATACCGTCCTAACTTGTAATAACAAAAGATTGCTATTTAATgg                                                                                                                                                                                         <  1:287387/219‑1 (MQ=255)                                                                                               tcttcATATTCTGATTTTAGTTTAGAGAGTTTAAACGCAGTATCCTTAAACCGGTTTTAAAAGATCCCGGTCAGTATTCTAATATGATTGTTAGCATTAAAACAAAAGGTCGTCCTGTTAGATAATTGATCTTTATATTGCCTTATTGGTGGCATTATCTCTAAATACCGTCCTAACTTGTAATAACAAAAGATTGCTATTTAATGGTCCCTGAAATTTTATTAGCTTGCTCAACAAt                                                                                                                                                          >  1:47630/1‑238 (MQ=255)                                                                                                                 agtttagAGAGTTTAAACGCAGTATCCTTAAACCGGTTTTAAAAGATCCCGGTCAGTATTCTAATATGATTGTTAGCATTAAAACAAAAGGTCGTCCTGTTAGATAATTGATCTTTATATTGCCTTATTGGTGGCATTATCTCTAAATACCGTCCTAACTTGTAATAACAAAAGATTGCTATTTAATGGTCCCTGAAATTTTATTAGCTTGCTCAACAATAg                                                                                                                                                        >  1:850648/1‑222 (MQ=255)                                                                                                                 agtttagAGAGTTTAAACGCAGTATCCTTAAACCGGTTTTAAAAGATCCCGGTCAGTATTCTAATATGATTGTTAGCATTAAAACAAAAGGTCGTCCTGTTAGATAATTGATCTTTATATTGCCTTATTGGTGGCATTATCTCTAAATACCGTCCTAACTTGTAATAACAAAAGATTGCTATTTAATGGTCCCTGAAATTTTATTAGCTTGCTCAACAATAGTTCACATTGAAACACTTCACGCATTAAtt                                                                                                                           >  1:575410/1‑251 (MQ=255)                                                                                                                 agtttagAGAGTTTAAACGCAGTATCCTTAAACCGGTTTTAAAAGATCCCGGTCAGTATTCTAATATGATTGTTAGCATTAAAACAAAAGGTCGTCCTGTTAGATAATTGATCTTTATATTGCCTTATTGGTGGCATTATCTCTAAATACCGTCCTAACTTGTAATAACAAAAGATTGCTATTTAATGGTCCCTGAAATTTTATTAGCTTGCTCAACAATAGTTCACATTGAAACACTTCACGCATTAAtt                                                                                                                           >  1:891914/1‑251 (MQ=255)                                                                                                                           gTTTAAACGCAGTATCCTTAAACCGGTTTTAAAAGATCCCGGTCAGTATTCTAATATGATTGTTAGCATTAAAACAAAAGGTCGTCCTGTTAGATAATTGATCTTTATATTGCCTTATTGGTGGCATTATCTCTAAATACCGTCCTAACTTGTAATAACAAAAGATTGCTATTTAAt                                                                                                                                                                                           <  1:87453/177‑1 (MQ=255)                                                                                                                           gTTTAAACGCAGTATCCTTAAACCGGTTTTAAAAGATCCCGGTCAGTATTCTAATATGATTGTTAGCATTAAAACAAAAGGTCGTCCTGTTAGATAATTGATCTTTATATTGCCTTATTGGTGGCATTATCTCTAAATACCGTCCTAACTTGTAATAACAAAAGATTGCTATTTAATGGTCCCTGAAATTTTATTAGCTTGCTCAACAAt                                                                                                                                                          <  1:597473/210‑1 (MQ=255)                                                                                                                           gTTTAAACGCAGTATCCTTAAACCGGTTTTAAAAGATCCCGGTCAGTATTCTAATATGATTGTTAGCATTAAAACAAAAGGTCGTCCTGTTAGATAATTGATCTTTATATTGCCTTATTGGTGGCATTATCTCTAAATACCGTCCTAACTTGTAATAACAAAAGATTGCTATTTAATGGTCCCTGAAATTTTATTAGCTTGCTCAACAATAGTTCACATTGAAacac                                                                                                                                         <  1:489676/227‑1 (MQ=255)                                                                                                                                  cGCAGTATCCTTAAACCGGTTTTAAAAGATCCCGGTCAGTATTCTAATATGATTGTTAGCATTAAAACAAAAGGTCGTCCTGTTAGATAATTGATCTTTATATTGCCTTATTGGTGGCATTATCTCTAAATACCGTCCTAACTTGTAATAACAAAAGATTGCTATTTAATgg                                                                                                                                                                                         >  1:800648/1‑172 (MQ=255)                                                                                                                                    cAGTATCCTTAAACCGGTTTTAAAAGATCCCGGTCAGTATTCTAATATGATTGTTAGCATTAAAACAAAAGGTCGTCCTGTTAGATAATTGATCTTTATATTGCCTTATTGGTGGCATTATCTCTAAATAc                                                                                                                                                                                                                                >  1:113440/1‑131 (MQ=255)                                                                                                                                    cAGTATCCTTAAACCGGTTTTAAAAGATCCCGGTCAGTATTCTAATATGATTGTTAGCATTAAAACAAAAGGTCGTCCTGTTAGATAATTGATCTTTATATTGCCTTATTGGTGGCATTATCTCTAAATACCGTCCTAACTTGTAATAACAAAAGATTGCTATTTAATGGTCCCTGAAATTTTATTAGCTTGCTCAACAATAGTTCACATTGAAACACTTCACGCATTAATTCAAACAGAAAGCTCatat                                                                                                         <  1:325197/250‑1 (MQ=255)                                                                                                                                    cAGTATCCTTAAACCGGTTTTAAAAGATCCCGGTCAGTATTCTAATATGATTGTTAGCATTAAAACAAAAGGTCGTCCTGTTAGATAATTGATCTTTATATTGCCTTATTGGTGGCATTATCTCTAAATACCGTCCTAACTTGTAATAACAAAAGATTGCTATTTAATGGTCCCTGAAATTTTATTAGCTTGCTCAACAATAGTTCACATTGAAACACTTCACGCATTAATTCAAACAGAAAGCTCAtata                                                                                                        >  1:366238/1‑251 (MQ=255)                                                                                                                                        aTCCTTAAACCGGTTTTAAAAGATCCCGGTCAGTATTCTAATATGATTGTTAGCATTAAAACAAAAGGTCGTCCTGTTAGATAATTGATCTTTATATTGCCTTATTGGTGGCATTATCTCTAAATACCGTCCTAACTTGTAATAACAAAAGATTGCTATTTAATGGTCCCTGAAATTTTATTAGCTTGCTCAACAATAGTTCACATTGAAACACTTCACGCATTAATTc                                                                                                                          <  1:847097/229‑1 (MQ=255)                                                                                                                                          ccTTAAACCGGTTTTAAAAGATCCCGGTCAGTATTCTAATATGATTGTTAGCATTAAAACAAAAGGTCGTCCTGTTAGATAATTGATCTTTATATTGCCTTATTGGTGGCATTATCTCTAAATACCGTCCTAACTTGTAATAACAAAAGATTGCTATTTAATGGTCCCTGAAATTTTATTAGCTTGCTCAACAATAg                                                                                                                                                        >  1:846868/1‑197 (MQ=255)                                                                                                                                          ccTTAAACCGGTTTTAAAAGATCCCGGTCAGTATTCTAATATGATTGTTAGCATTAAAACAAAAGGCCGTCCTGTTAGATAATTGATCTTTATATTGCCTTATTGGTGGCATTATCTCTAAATACCGTCCTAACTTGTAATAACAAAAGATTGCTATTTAATGGTCCCTGAAATTTTATTAGCTTGCTCAACAATAGTTCACATTGAAACACTTCACGCATTAATTCAAACAGAAAGCTCATATAATCCtt                                                                                                  >  1:849961/1‑251 (MQ=255)                                                                                                                                              aaaCCGGTTTTAAAAGATCCCGGTCAGTATTCTAATATGATTGTTAGCATTAAAACAAAAGGTCGTCCTGTTAGATAATTGATCTTTATATTGCCTTATTGGTGGCATTATCTCTAAATACCGTCCTAACTTGTAATAACAAAAGATTGCTATTTAATGGTCCCTGAAATTTTATTAGCTTGCTCAACAATAGTTCACATTGAAACACTTCACGCATTAATTCAAACAGAAAGCTCATATAATCCTTATGc                                                                                              <  1:208997/251‑1 (MQ=255)                                                                                                                                                         aaaaGATCCCGGTCAGTATTCTAATATGATTGTTAGCATTAAAACAAAAGGTCGTCCTGTTAGATAATTGATCTTTATATTGCCTTATTGGTGGCATTATCTCTAAATACCGTCCTAACTTGTAATAACAAAAGATTGCTATTTAATGGTCCCTGAAATTTTATTAGCTTGCTCAACAATAGTTCACATTGAAACACTTCACGCATTAATTCAAACAGAAAGCTCATATAATc                                                                                                     <  1:165890/233‑1 (MQ=255)                                                                                                                                                                      cAGTATTCTAATATGATTGTTAGCATTAAAACAAAAGGTCGTCCTGTTAGATAATTGATCTTTATATTGCCTTATTGGTGGCATTATCTCTAAATACCGTCCTAACTTGTAATAACAAAAGATTGCTATTTAATGGTCCCTGAAATTTTATTAGCTTGCTCAACAATAGTTCACATTGAAACACTTCACGCATTAATTCAAACAGAAAGCTCATATAATcc                                                                                                    >  1:220615/1‑221 (MQ=255)                                                                                                                                                                        gTATTCTAATATGATTGTTAGCATTAAAACAAAAGGTCGTCCTGTTAGATAATTGATCTTTATATTGCCTTATTGGTGGCATTATCTCTAAATACCGTCCTAACTTGTAATAACAAAAGATTGCTATTTAATGGTCCCTGAAATTTTATTAGCTTGCTCAACAAt                                                                                                                                                          <  1:586898/165‑1 (MQ=255)                                                                                                                                                                        gTATTCTAATATGATTGTTAGCATTAAAACAAAAGGTCGTCCTGTTAGATAATTGATCTTTATATTGCCTTATTGGTGGCATTATCTCTAAATACCGTCCTAACTTGTAATAACAAAAGATTGCTATTTAATGGTCCCTGAAATTTTATTAGCTTGCTCAACAATAGTTCACATTGAAACACTTCACGCATTAATTCAAACAGAAAGCTCATATaa                                                                                                       >  1:432446/1‑216 (MQ=255)                                                                                                                                                                        gTATTCTAATATGATTGTTAGCATTAAAACAAAAGGTCGTCCTGTTAGATAATTGATCTTTATATTGCCTTATTGGTGGCATTATCTCTAAATACCGTCCTAACTTGTAATAACAAAAGATTGCTATTTAATGGTCCCTGAAATTTTATTAGCTTGCTCAACAATAGTTCACATTGAAACACTTCACGCATTAATTCAAACAGAAAGCTCATATAATCCTTATGCTATCGCAGTAGTAAATGATATTCct                                                                     >  1:80350/1‑250 (MQ=255)                                                                                                                                                                        gTATTCTAATATGATTGTTAGCATTAAAACAAAAGGTCGTCCTGTTAGATAATTGATCTTTATATTGCCTTATTGGTGGCATTATCTCTAAATACCGTCCTAACTTGTAATAACAAAAGATTGCTATTTAATGGTCCCTGAAATTTTATTAGCTTGCTCAACAATAGTTCACATTGAAACACTTCACGCATTAATTCAAACAGAAAGCTCATATAATCCTTATGCTATCGCAGTAGTAAATGATATTCct                                                                     >  1:145920/1‑250 (MQ=255)                                                                                                                                                                          aTTCTAATATGATTGTTAGCATTAAAACAAAAGGTCGTCCTGTTAGATAATTGATCTTTATATTGCCTTATTGGTGGCATTATCTCTAAATACCGTCCTAACTTGTAATAACAAAAGATTGCTATTTAATGGTCCCTGAAATTTTATTAGCTTGCTCAACAATAGTTCACATTGAAACACTTCACGCATTAATTCAAACAGAAAGCTCATATAATCCTTATGCTATCGCAGTAGTAAATGATATTCCTCtt                                                                  >  1:2113/1‑251 (MQ=255)                                                                                                                                                                           ttCTAATATGATTGTTAGCATTAAAACAAAAGGTCGTCCTGTTAGATAATTGATCTTTATATTGCCTTATTGGTGGCATTATCTCTAAATACCGTCCTAACTTGTAATAACAAAAGATTGCTATTTAATGGTCCCTGAAATTTTATTAGCTTGCTCAACAATAGTTCACAtt                                                                                                                                                >  1:376897/1‑172 (MQ=255)                                                                                                                                                                                atatGATTGTTAGCATTAAAACAAAAGGTCGTCCTGTTAGATAATTGATCTTTATATTGCCTTATTGGTGGCATTATCTCTAAATACCGTCCTAACTTGTAATAACAAAAGATTGCTATTTAATGGTCCCTGAAATTTTATTAGCTTGCTCAACAATAGTTCACATTGAAACACTTCACGCATTAATTCAAACAGAAAGCTCATATaa                                                                                                       <  1:225365/208‑1 (MQ=255)                                                                                                                                                                                atatGATTGTTAGCATTAAAACAAAAGGTCGTCCTGTTAGATAATTGATCTTTATATTGCCTTATTGGTGGCATTATCTCTAAATACCGTCCTAACTTGTAATAACAAAAGATTGCTATTTAATGGTCCCTGAAATTTTATTAGCTTGCTCAACAATAGTTCACATTGAAACACTTCACGCATTAATTCAAACAGAAAGCTCATATAATCCTTATGCTATCGCAGTAGTAAATGATATTCCTCTTGCTCaa                                                            >  1:49973/1‑251 (MQ=255)                                                                                                                                                                                            gCATTAAAACAAAAGGTCGTCCTGTTAGATAATTGATCTTTATATTGCCTTATTGGTGGCATTATCTCTAAATACCGTCCTAACTTGTAATAACAAAAGATTGCTATTTAATGGTCCCTGAAATTTTATTAGCTTGCTCAACAATAGTTCACATTGAAACACTTCACGCATTAATTCAAACAGAAAGCTCATATAATCCTTATGCTATCGCAGTAGTAAATGATATTCCTCTTGCTCAACAGCCTAAAaca                                                >  1:833443/1‑251 (MQ=255)                                                                                                                                                                                             cATTAAAACAAAAGGTCGTCCTGTTAGATAATTGATCTTTATATTGCCTTATTGGTGGCATTATCTCTAAATACCGTCCTAACTTGTAATAACAAAAGATTGCTATTTAATGGTCCCTGAAATTTTATTAGCTTGCTCAACAATAGTTCACATTGAAacac                                                                                                                                         >  1:224594/1‑161 (MQ=255)                                                                                                                                                                                             cATTAAAACAAAAGGTCGTCCTGTTAGATAATTGATCTTTATATTGCCTTATTGGTGGCATTATCTCTAAATACCGTCCTAACTTGTAATAACAAAAGATTGCTATTTAATGGTCCCTGAAATTTTATTAGCTTGCTCAACAATAGTTCACATTGAAACACTTCACGCATTAATTCAAACAGAAAGCTCATATAATCCTTATGCTATCGCAGTAGTAAATGATATTTCTCTTGCTCAACAGCCTAAAac                                                 <  1:809628/249‑1 (MQ=255)                                                                                                                                                                                                  aaaCAAAAGGTCGTCCTGTTAGATAATTGATCTTTATATTGCCTTATTGGTGGCATTATCTCTAAATACCGTCCTAACTTGTAATAACAAAAGATTGCTATTTAATGGTCCCTGAAATTTTATTAGCTTGCTCAACAATAGTTCACATTGAAACACTTCACGCATTAATTCAAACAGAAAGCTCATATAATcc                                                                                                    >  1:338902/1‑193 (MQ=255)                                                                                                                                                                                                  aaaCAAAAGGTCGTCCTGTTAGATAATTGATCTTTATATTGCCTTATTGGTGGCATTATCTCTAAATACCGTCCTAACTTGTAATAACAAAAGATTGCTATTTAATGGTCCCTGAAATTTTATTAGCTTGCTCAACAATAGTTCACATTGAAACACTTCACGCATTAATTCAAACAGAAAGCTCATATAATcc                                                                                                    >  1:95462/1‑193 (MQ=255)                                                                                                                                                                                                   aaCAAAAGGTCGTCCTGTTAGATAATTGATCTTTATATTGCCTTATTGGTGGCATTATCTCTAAATACCGTCCTAACTTGTAATAACAAAAGATTGCTATTTAATGGTCCCTGAAATTTTATTAGCTTGCTCAACAATAGTTCACATTGAAACACTTCACGCATTAATTCAAACAGAAAGCTCATATaa                                                                                                       <  1:679157/189‑1 (MQ=255)                                                                                                                                                                                                                 cTGTTAGATAATTGATCTTTATATTGCCTTATTGGTGGCATTATCTCTAAATACCGTCCTAACTTGTAATAACAAAAGATTGCTATTTAATGGTCCCTGAAATTTTATTAGCTTGCTCAACAATAGTTCACATTGAAACACTTCACGCATTAATTCAAACAGAAAGCTCATATAATCCTTATGc                                                                                              <  1:707129/184‑1 (MQ=255)                                                                                                                                                                                                                 cTGTTAGATAATTGATCTTTATATTGCCTTATTGGTGGCATTATCTCTAAATACCGTCCTAACTTGTAATAACAAAAGATTGCTATTTAATGGTCCCTGAAATTTTATTAGCTTGCTCAACAATAGTTCACATTGAAACACTTCACGCATTAATTCAAACAGAAAGCTCATATAATCCTTATGCTATCGCAGTAGTAAAt                                                                              <  1:13475/200‑1 (MQ=255)                                                                                                                                                                                                                 cTGTTAGATAATTGATCTTTATATTGCCTTATTGGTGGCATTATCTCTAAATACCGTCCTAACTTGTAATAACAAAAGATTGCTATTTAATGGTCCCTGAAATTTTATTAGCTTGCTCAACAATAGTTCACATTGAAACACTTCACGCATTAATTCAAACAGAAAGCTCATATAATCCTTATGCTATCGCAGTAGTAAATGATATTCCTCTTGCTCAACAGCCTAAAACACTCCAAGAAGCAGAAt                                <  1:317684/246‑1 (MQ=255)                                                                                                                                                                                                                   gTTAGATAATTGATCTTTATATTGCCTTATTGGTGGCATTATCTCTAAATACCGTCCTAACTTGTAATAACAAAAGATTGCTATTTAATGGTCCCTGAAATTTTATTAGCTTGCTCAACAATAGTTCACATTGAAACACTTCACGCATTAATTCAAACAGAAAGCTCATATAATcc                                                                                                    >  1:866067/1‑176 (MQ=255)                                                                                                                                                                                                                   gTTAGATAATTGATCTTTATATTGCCTTATTGGTGGCATTATCTCTAAATACCGTCCTAACTTGTAATAACAAAAGATTGCTATTTAATGGTCCCTGAAATTTTATTAGCTTGCTCAACAATAGTTCACATTGAAACACTTCACGCATTAATTCAAACAGAAAGCTCATATAATCCTTATGCTATCGCAGTAGTAAATGATATTCCTCTTGCTCAACAGCCTAAAACACTCCAAGAAGCAGAATTAGTCAt                         >  1:391124/1‑251 (MQ=255)                                                                                                                                                                                                                      aGATAATTGATCTTTATATTGCCTTATTGGTGGCATTATCTCTAAATACCGTCCTAACTTGTAATAACAAAAGATTGCTATTTAATGGTCCCTGAAATTTTATTAGCTTGCTCAACAATAGTTCACATTGAAACACTTCACg                                                                                                                                   >  1:624344/1‑142 (MQ=255)                                                                                                                                                                                                                      aGATAATTGATCTTTATATTGCCTTATTGGTGGCATTATCTCTAAATACCGTCCTAACTTGTAATAACAAAAGATTGCTATTTAATGGTCCCTGAAATTTTATTAGCTTGCTCAACAATAGTTCACATTGAAACACTTCACGCATTAATTCAAACAGAAAGCTCATATAATCCTTATGCTATCGCAGTAGTAAATGATATTCCTCTTGCTCAACAGCCTAAAACACTCCAAGAAGCAGAATTAGTCATAGa                      >  1:238100/1‑251 (MQ=255)                                                                                                                                                                                                                       gATAATTGATCTTTATATTGCCTTATTGGTGGCATTATCTCTAAATACCGTCCTAACTTGTAATAACAAAAGATTGCTATTTAATGGTCCCTGAAATTTTATTAGCTTGCTCAACAATAGTTCACATTGAAACACTTCACGCATTAATTCAAACAGAAAGc                                                                                                               >  1:874300/1‑161 (MQ=255)                                                                                                                                                                                                                        aTAATTGATCTTTATATTGCCTTATTGGTGGCATTATCTCTAAATACCGTCCTAACTTGTAATAACAAAAGATTGCTATTTAATGGTCCCTGAAATTTTATTAGCTTGCTCAACAATAGTTCACATTGAAACACTTCACGCATTAATTCAAACAGAAAGCTCATATAATCCTTATGCTATCGCAGTAGTAAATGATATTCCTCTTGCTCAACAGCCTAAAACACTCCAAGAAGCAGAATTAGTCATAGAt                     <  1:526500/250‑1 (MQ=255)                                                                                                                                                                                                                         tAATTGATCTTTATATTGCCTTATTGGTGGCATTATCTCTAAATACCGTCCTAACTTGTAATAACAAAAGATTGCTATTTAATGGTCCCTGAAATTTTATTAGCTTGCTCAACAATAGTTCACATTGAAACACTTCACGCATTAATTCAAACAGAAAGCTCATATAATc                                                                                                     >  1:620312/1‑169 (MQ=255)                                                                                                                                                                                                                           aTTGATCTTTATATTGCCTTATTGGTGGCATTATCTCTAAATACCGTCCTAACTTGTAATAACAAAAGATTGCTATTTAATGGTCCCTGAAATTTTATTAGCTTGCTCAACAATAGTTCACATTGAAACACTTCACGCATTAATTCAAACAGAAAGCTCATATAATCCTTATGCTATCGCAGTAGTAAATGATATTCCTCTTGCTCAACAGCCTAAAACACTCCAAGAAGCAGAATTAGTCATAGATGAAc                 <  1:828113/251‑1 (MQ=255)                                                                                                                                                                                                                                tCTTTATATTGCCTTATTGGTGGCATTATCTCTAAATACCGTCCTAACTTGTAATAACAAAAGATTGCTATTTAATGGTCCCTGAAATTTTATTAGCTTGCTCAACAATAGTTCACATTGAAACACTTCACGCATTAATTCAAACAGAAAGCTCATATAATCCTTATGCTATCGCAGTAGTAAATGATATTCCTCTTGCTCAACAGCCTAAAACACTCCaagaa                                       >  1:542298/1‑224 (MQ=255)                                                                                                                                                                                                                                tCTTTATATTGCCTTATTGGTGGCATTATCTCTAAATACCGTCCTAACTTGTAATAACAAAAGATTGCTATTTAATGGTCCCTGAAATTTTATTAGCTTGCTCAACAATAGTTCACATTGAAACACTTCACGCATTAATTCAAACAGAAAGCTCATATAATCCTTATGCTATCGCAGTAGTAAATGATATTCCTCTTGCTCAACAGCCTAAAACACTCCAAGAAGCAGAATTAGTCATAGATGAACTTGaa            >  1:781788/1‑251 (MQ=255)                                                                                                                                                                                                                                tCTTTATATTGCCTTATTGGTGGCATTATCTCTAAATACCGTCCTAACTTGTAATAACAAAAGATTGCTATTTAATGGTCCCTGAAATTTTATTAGCTTGCTCAACAATAGTTCACATTGAAACACTTCACGCATTAATTCAAACAGAAAGCTCATATAATCCTTATGCTATCGCAGTAGTAAATGATATTCCTCTTGCTCAACAGCCTAAAACACTCCAAGAAGCAGAATTAGTCATAGATGAACTTGaa            >  1:730719/1‑251 (MQ=255)                                                                                                                                                                                                                                   ttATATTGCCTTATTGGTGGCATTATCTCTAAATACCGTCCTAACTTGTAATAACAAAAGATTGCTATTTAATGGTCCCTGAAATTTTATTAGCTTGCTCAACAATAGTTCACATTGAAACACTTCACg                                                                                                                                   <  1:676120/129‑1 (MQ=255)                                                                                                                                                                                                                                   ttATATTGCCTTATTGGTGGCATTATCTCTAAATACCGTCCTAACTTGTAATAACAAAAGATTGCTATTTAATGGTCCCTGAAATTTTATTAGCTTGCTCAACAATAGTTCACATTGAAACACTTCACGCATTAATTCAAACAGAAAGCTCATATAATCCTTATGCTATCGCAGTAGTAAATGATATTCCTCTTGCTCAACAGCCTAAAACACTCCAAGAAGCAGAATTAGTCATAGATGAACTTGAAg           >  1:213763/1‑249 (MQ=255)                                                                                                                                                                                                                                    tataTTGCCTTATTGGTGGCATTATCTCTAAATACCGTCCTAACTTGTAATAACAAAAGATTGCTATTTAATGGTCCCTGAAATTTTATTAGCTTGCTCAACAAt                                                                                                                                                          >  1:464239/1‑105 (MQ=255)                                                                                                                                                                                                                                     atatTGCCTTATTGGTGGCATTATCTCTAAATACCGTCCTAACt                                                                                                                                                                                                                      <  1:157033/44‑1 (MQ=255)                                                                                                                                                                                                                                          gCCTTATTGGTGGCATTATCTCTAAATACCGTCCTAACTTGTAATAACAAAAGATTGCTATTTAATGGTCCCTGAAATTTTATTAGCTTGCTCAACAATAGTTCACATTGAAACACTTCACGCa                                                                                                                                 <  1:690847/124‑1 (MQ=255)                                                                                                                                                                                                                                          gCCTTATTGGTGGCATTATCTCTAAATACCGTCCTAACTTGTAATAACAAAAGATTGCTATTTAATGGTCCCTGAAATTTTATTAGCTTGCTCAACAATAGTTCACATTGAAACACTTCACGCa                                                                                                                                 >  1:148671/1‑124 (MQ=255)                                                                                                                                                                                                                                          gCCTTATTGGTGGCATTATCTCTAAATACCGTCCTAACTTGTAATAACAAAAGATTGCTATTTAATGGTCCCTGAAATTTTATTAGCTTGCTCAACAATAGTTCACATTGAAACACTTCACGCATTAATTCAAACAGAAAGCTCATATAATCCTTATg                                                                                               <  1:741022/158‑1 (MQ=255)                                                                                                                                                                                                                                          gCCTTATTGGTGGCATTATCTCTAAATACCGTCCTAACTTGTAATAACAAAAGATTGCTATTTAATGGTCCCTGAAATTTTATTAGCTTGCTCAACAATAGTTCACATTGAAACACTTCACGCATTAATTCAAACAGAAAGCTCATATAATCCTTATGCTATCGCAGTAGTAAATGATATTCCTCTTGCTCAAc                                                           <  1:9712/194‑1 (MQ=255)                                                                                                                                                                                                                                          gCCTTATTGGTGGCATTATCTCTAAATACCGTCCTAACTTGTAATAACAAAAGATTGCTATTTAATGGTCCCTGAAATTTTATTAGCTTGCTCAACAATAGTTCACATTGAAACACTTCACGCATTAATTCAAACAGAAAGCTCATATAATCCTTATGCTATCGCAGTAGTAAATGATATTCCTCTTGCTCAACAGCCTAAAACACTCCAAGAAGCAGAATTAGTCATAGATGAACTTGAAGCTaaaaaaa  >  1:278218/1‑251 (MQ=255)                                                                                                                                                                                                                                              tATTGGTGGCATTATCTCTAAATACCGTCCTAACTTGTAATAACAAAAGATTGCTATTTAATGGTCCCTGAAATTTTATTAGCTTGCTCAACAATAGTTCACATTGAAACACTTCACGCATTAATTCAAACAGAAAGCTCATATAATCCTTATGCTATCGCAGTAGTAAATGATATTCCTCTTGCTCAACAGCCTAAAac                                                 <  1:90725/200‑1 (MQ=255)                                                                                                                                                                                                                                               aTTGGTGGCATTATCTCTAAATACCGTCCTAACTTGTAATAACAAAAGATTGCTATTTAATGGTCCCTGAAATTTTATTAGCTTGCTCAACAATAGTTCACATTGAAACACTTCACGCATTAATTc                                                                                                                          >  1:508554/1‑126 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                  AATATGGATCAATCGTTAGACTTAGTATTTATTGAATATGTTCAACCCTATTTATTCAAATTGAAAAAGTTTACTTAATAATGATGGAGAAAGTTCTTCATATTCTGATTTTAGTTTAGAGAGTTTAAACGCAGTATCCTTAAACCGGTTTTAAAAGATCCCGGTCAGTATTCTAATATGATTGTTAGCATTAAAACAAAAGGTCGTCCTGTTAGATAATTGATCTTTATATTGCCTTATTGATGGCATTATCTCTAAATACCGTCCTAACTTGTAATAACAAAAGATTGCTATTTAATGGTCCCTGAAATTTTATTAGCTTGCTCAACAATAGTTCACATTGAAACACTTCACGCATTAATTCAAACAGAAAGCTCATATAATCCTTATGCTATCGCAGTAGTAAATGATATTCCTCTTGCTCAACAGCCTAAAACACTCCAAGAAGCAGAATTAGTCATAGATGAACTTGAAGCTAAAAAAA  >  1/2049475‑2049958

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑