Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	2,038,596	A→G	S104P (TCG→CCG)	unknown ←	Lysine decarboxylase family

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	2,038,596	0	A	G	100.0%	176.6 / NA	67	S104P (TCG→CCG)	unknown	Lysine decarboxylase family
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (39/28);  total (39/28)

ATCTCAGGCACTTGATAATCAAGAATAAAACGTTCTATTTCAAAAATATTTGTACTAAATAATAACTTATCAAAATGACTTTGCGTCAAGAAACCGGCAGTGACCATGCTTTGATATTGACTTCTCAGCGGTTCATAAAAACCATCTTGGTTAAATAGAATACAAGGATGAGGATTTTTACCGATTCGCGCCCAAGAATAGACTTCGCTGATTTCTTCCAGCGTGCCCGGCCCACCCGACAAAGCAATACAAGCATCGGCTAATGCCAGCATTTTGGCTTTTCGCTCCGACATGGAATCCACAATGATAATTTCGGTACAACCTTGGTGTACCAATTCCCGCGCCTGTAAAAATTCAGGGATCACGCCGATAACGGTACCGCCGTTGGAAAGTACCGTATCCGCCAACACGCCCATCAATCCCGCCTTTCCGCCGCCATAAACTAACGTATGTCGATGTTGCGTAATCCATTCGCC  >  1/2038358‑2038833                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                              aTCTCAGACACTTGATAATCAAGAATAAAACGTTCTATTTCAAAAATATTTGTACTAAATAATAACTTATCAAAATGACTTTGCGTCAAGAAACCGGCAGTGACCATGCTTTGATATTGACTTCTCAGCGGTTCATAAAAACCATCTTGGTTAAATAGAATACAAGGATGAGGATTTTTACCGATTCGCGCCCAAGAATAGACTTCGCTGATTTCTTCCAGCGTGCCCGGCCCACCCGGCAAAGCAATACa                                                                                                                                                                                                                                   >  1:698375/1‑251 (MQ=255)           cTTGATAATCAAGAATAAAACGTTCTATTTCAAAAATATTTGTACTAAATAATAACTTATCAAAATGACTTTGCGTCAAGAAACCGGCAGTGACCATGCTTTGATATTGACTTCTCAGCGGTTCATAAAAACCATCTTGGTTAAATAGAATACAAGGATGAGGATTTTTACCGATTCGCGCCCAAGAATAGACTTCGCTGATTTCTTCCAGCGTGCCCGGCCCACCCGGCAAAGCAATACAAGCATCgg                                                                                                                                                                                                                           >  1:866047/1‑249 (MQ=255)                tAATCAAGAATAAAACGTTCTATTTCAAAAATATTTGTACTAAATAATAACTTATCAAAATGACTTTGCGTCAAGAAACCGGCAGTGACCATGCTTTGATATTGACTTCTCAGCGGTTCATAAAAACCATCTTGGTTAAATAGAATACAAGGATGAGGATTTTTACCGATTCGCGCCCAAGAATAGACTTCGCTGATTTCTTCCAGCGTGCCCGGCCCACCCGGCAAAGCAATACAAGCATCGGCTAATGc                                                                                                                                                                                                                    <  1:226154/251‑1 (MQ=255)                                                     tACTAAATAATAACTTATCAAAATGACTTTGCGTCAAGAAACCGGCAGTGACCATGCTTTGATATTGACTTCTCAGCGGTTCATAAAAACCATCTTGGTTAAATAGAATACAAGGATGAGGATTTTTACCGATTCGCGCCCAAGAATAGACTTCGCTGATTTCTTCCAGCGTGCCCGGCCCACCCGGCAAAGCAATACAAGCATCGGCTAATGCCAGCATTTTGGCTTTTCGCTCCGACATGGAATCcac                                                                                                                                                                                <  1:343721/250‑1 (MQ=255)                                                         aaaTAATAACTTATCAAAATGACTTTGCGTCAAGAAACCGGCAGTGACCATGCTTTGATATTGACTTCTCAGCGGTTCATAAAAACCATCTTGGTTAAATAGAATACAAGGATGAGGATTTTTACCGATTCGCGCCCAAGAATAGACTTCGCTGATTTCTTCCAGCGTGCCCGGCCCACCCGGCAAAGCAATACAAGCATCGGCTAATGCCAGCATTTTGGCTTTTCGCTCCGACATGGAATCCACAATGa                                                                                                                                                                           >  1:610220/1‑251 (MQ=255)                                                         aaaTAATAACTTATCAAAATGACTTTGCGTCAAGAAACCGGCAGTGACCATGCTTTGATATTGACTTCTCAGCGGTTCATAAAAACCATCTTGGTTAAATAGAATACAAGGATGAGGATTTTTACCGATTCGCGCCC‑AGAATAGACTTCGCTGATTTCTTCCAGCGTGCCCGGCCCACCCGGCAAAGCAATACAAGCATCGGCTAATGCCAGCATTTTGGCTTTTCGCTCCGACATGGAATCCACAATGAt                                                                                                                                                                          >  1:771536/1‑251 (MQ=255)                                                          aataatAACTTATCAAAATGACTTTGCGTCAAGAAACCGGCAGTGACCATGCTTTGATATTGACTTCTCAGCGGTTCATAAAAACCATCTTGGTTAAATAGAATACAAGGATGAGGATTTTTACCGATTCGCGCCCAAGAATAGACTTCGCTGATTTCTTCCAGCGTGCCCGGCCCACCCGGCAAAGCAATACAAGCATCGGCTAATGCCAGCATTTTGGCTTTTCGCTCCGACATGGAATCCACAATGAt                                                                                                                                                                          >  1:466700/1‑251 (MQ=255)                                                           ataataACTTATCAAAATGACTTTGCGTCAAGAAACCGGCAGTGACCATGCTTTGATATTGACTTCTCAGCGGTTCATAAAAACCATCTTGGTTAAATAGAATACAAGGATGAGGATTTTTACCGATTCGCGCCCAAGAATAGACTTCGCTGATTTCTTCCAGCGTGCCCGGCCCACCCGGCAAAGCAATACAAGCATCGGCTAATGCCAGCATTTTGGCTTTTCGCTCCGACATGGAATCCACAATGATa                                                                                                                                                                         >  1:286669/1‑251 (MQ=255)                                                                aaCTTATCAAAATGACTTTGCGTCAAGAAACCGGCAGTGACCATGCTTTGATATTGACTTCTCAGCGGTTCATAAAAACCATCTTGGTTAAATAGAATACAAGGATGAGGATTTTTACCGATTCGCGCCCAAGAATAGACTTCGCTGATTTCTTCCAGCGTGCCCGGCCCACCCGGCAAAGCAATACAAGCATCGGCTAATGCCAGCATTTTGGCTTTTCGCTCCGACATGGAATCCACAATGATAATTTc                                                                                                                                                                    <  1:470395/251‑1 (MQ=255)                                                                       cAAAATGACTTTGCGTCAAGAAACCGGCAGTGACCATGCTTTGATATTGACTTCTCAGCGGTTCATAAAAACCATCTTGGTTAAATAGAATACAAGGATGAGGATTTTTACCGATTCGCGCCCAAGAATAGACTTCGCTGATTTCTTCCAGCGTGCCCGGCCCACCCGGCAAAGCAATACAAGCATCGGCTAATGCCAGCATTTTGGCTTTTCGCTCCGACATGGAATCCACAATGATAATTTCGGTACaa                                                                                                                                                             >  1:774213/1‑251 (MQ=255)                                                                                          gAAACCGGCAGTGACCATGCTTTGATATTGACTTCTCAGCGGTTCATAAAAACCATCTTGGTTAAATAGAATACAAGGATGAGGATTTTTACCGATTCGCGCCCAAGAATAGACTTCGCTGATTTCTTCCAGCGTGCCCGGCCCACCCGGCAAAGCAATACAAGCATCGGCTAATGCCAGCATTTTGGCTTTTCGCTCCGACATGGAATCCACAATGATAATTTCGGTACAACCTTGGTGTACCAATTcc                                                                                                                                           >  1:712492/1‑250 (MQ=255)                                                                                           aaaCCGGCAGTGACCATGCTTTGATATTGACTTCTCAGCGGTTCATAAAAACCATCTTGGTTAAATAGAATACAAGGATGAGGATTTTTACCGATTCGCGCCCAAGAATAGACTTCGCTGATTTCTTCCAGCGTGCCCGGCCCACCCGGCAAAGCAATACAAGCATCGGCTAATGCCAGCATTTTGGCTTTTCGCTCCGACATGGAATCCACAATGATAATTTCGGTACAACCTTGGTGTACCAATTCCcg                                                                                                                                         <  1:514670/251‑1 (MQ=255)                                                                                            aaCCGGCAGTGACCATGCTTTGATATTGACTTCTCAGCGGTTCATAAAAACCATCTTGGTTAAATAGAATACAAGGATGAGGATTTTTACCGATTCGCGCCCAAGAATAGACTTCGCTGATTTCTTCCAGCGTGCCCGGCCCACCCGGCAAAGCAATACAAGCATCGGCTAATGCCAGCATTTTGGCTTTTCGCTCCGACATGGAATCCACAATGATAAtt                                                                                                                                                                      >  1:656044/1‑221 (MQ=255)                                                                                            aaCCGGCAGTGACCATGCTTTGATATTGACTTCTCAGCGGTTCATAAAAACCATCTTGGTTAAATAGAATACAAGGATGAGGATTTTTACCGATTCGCGCCCAAGAATAGACTTCGCTGATTTCTTCCAGCGTGCCCGGCCCACCCGGCAAAGCAATACAAGCATCGGCTAATGCCAGCATTTTGGCTTTTCGCTCCGACATGGAATCCACAATGATAATTTCGGTACAAcc                                                                                                                                                           >  1:609303/1‑232 (MQ=255)                                                                                                      gACCATGCTTTGATATTGACTTCTCAGCGGTTCATAAAAACCATCTTGGTTAAATAGAATACAAGGATGAGGATTTTTACCGATTCGCGCCCAAGAATAGACTTCGCTGATTTCTTCCAGCGTGCCCGGCCCACCCGGCAAAGCAATAc                                                                                                                                                                                                                                    <  1:71140/149‑1 (MQ=255)                                                                                                            gCTTTGATATTGACTTCTCAGCGGTTCATAAAAACCATCTTGGTTAAATAGAATACAAGGATGAGGATTTTTACCGATTCGCGCCCAAGAATAGACTTCGCTGATTTCTTCCAGCGTGCCCGGCCCACCCGGCAAAGCAATACAAGCATCGGCTAATGCCAGCATTTTGGCTTTTCGCTCCGACATGGAATCCACAATGATAATTTCGGTACAAcc                                                                                                                                                           <  1:223673/216‑1 (MQ=255)                                                                                                              tttGATATTGACTTCTCAGCGGTTCATAAAAACCATCTTGGTTAAATAGAATACAAGGATGAGGATTTTTACCGATTCGCGCCCAAGAATAGACTTCGCTGATTTCTTCCAGCGTGCCCGGCCCACCCGGCAAAGCAATACAAGCATCGGCTAATGCCAGCATTTTGGCTTTTCGCTCCGACATGGAATCCACAATGATAATTTCGGTACAACCTTGGTGTACCAATTCCCGCGCCTGTAAAAATTCAggg                                                                                                                      <  1:82677/251‑1 (MQ=255)                                                                                                                       gACTTCTCAGCGGTTCATAAAAACCATCTTGGTTAAATAGAATACAAGGATGAGGATTTTTACCGATTCGCGCCCAAGAATAGACTTCGCTGATTTCTTCCAGCGTGCCCGGCCCACCCGGCAAAGCAATACAAGCATCGGCTAATGCCAGCATTTTGGCTTTTCGCTCCGACATGGAATCCACAATGATAATTTCGGTACAACCTTGGTGTACCAATTCCCGCGCCTGTAAAAATTCAGGGATCACGCCg                                                                                                             >  1:832252/1‑251 (MQ=255)                                                                                                                        aCTTCTCAGCGGTTCATAAAAACCATCTTGGTTAAATAGAATACAAGGATGAGGATTTTTACCGATTCGCGCCCAAGAATAGACTTCGCTGATTTCTTCCAGCGTGCCCGGCCCACCCGGCAAAGCAATACAAGCATCGGCTAATGCCAGCATTTTGGCTTTTCGCTCCGACATgg                                                                                                                                                                                       >  1:241962/1‑176 (MQ=255)                                                                                                                           tctcAGCGGTTCATAAAAACCATCTTGGTTAAATAG‑ATACAAGGATGAGGATTTTTACCGATTCGCGCCCAAGAATAGACTTCGCTGATTTCTTCCAGCGTGCCCGGCCCACCCGGCAAAGCAATACAAGCATCGGCTAATGCCAGCATTTTGGCTTTTCGCTCCGACATGGAATCCACAATGATAATTTCGGTACAACCTTGGTGTACCAATTCCCGCGCCTGTAAAAATTCAGGGATCACGCCGATaa                                                                                                         >  1:895753/1‑250 (MQ=255)                                                                                                                                 cGGTTCATAAAAACCATCTTGGTTAAATAGAATACAAGGATGAGGATTTTTACCGATTCGCGCCCAAGAATAGACTTCGCTGATTTCTTCCAGCGTGCCCGGCCCACCCGGCAAAGCAATACAAGCATCGGCTAATGCCAGCATTTTGGCTTTTCGCTCCGACATGGAATCCACAATGATAATTTCGGTACAACCTTGGTGTACCAATTCCCGCGCCTGTAAAAATTCAGGGATCACGCCGATAACGGt                                                                                                     <  1:28835/249‑1 (MQ=255)                                                                                                                                      cATAAAAACCATCTTGGTTAAATAGAATACAAGGATGAGGATTTTTACCGATTCGCGCCCAAGAATAGACTTCGCTGATTTCTTCCAGCGTGCCCGGCCCACCCGGCAAAGCAATACAAGCATCGGCTAATGCCAGCATTTTGGCTTTTCGCTCCGACATgg                                                                                                                                                                                       <  1:114908/162‑1 (MQ=255)                                                                                                                                      cATAAAAACCATCTTGGTTAAATAGAATACAAGGATGAGGATTTTTACCGATTCGCGCCCAAGAATAGACTTCGCTGATTTCTTCCAGCGTGCCCGGCCCACCCGGCAAAGCAATACAAGCATCGGCTAATGCCAGCATTTTGGCTTTTCGCTCCGACATGGAATCCACAATGATAATTTCGGTACAACCTTGGTGTACCAATTCCCGCGCCTGTAAAAATTCAGGGATCACGCCGATAACGGTAccgccg                                                                                              >  1:888933/1‑251 (MQ=255)                                                                                                                                       aTAAAAACCATCTTGGTTAAATAGAATACAAGGATGAGGATTTTTACCGATTCGCGCCCAAGAATAGACTTCGCTGATTTCTTCCAGCGTGCCCGGCCCACCCGGCAAAGCAATACAAGCATCGGCTAATGCCAGCATTTTGGCTTTTCGCTCCGACATGGAATCCACAATGATAATTTCGGTACAACCTTGGTGTACCAATTCCCGCGCCTGTAAAAATTCAGGGATCACGCCGATAACGGTACCGCCGt                                                                                             >  1:459725/1‑251 (MQ=255)                                                                                                                                           aaaCCATCTTGGTTAAATAGAATACAAGGATGAGGATTTTTACCGATTCGCGCCCAAGAATAGACTTCGCTGATTTCTTCCAGCGTGCCCGGCCCACCCGGCAAAGCAATACAAGCATCGGCTAATGCCAGCATTTTGGCTTTTCGCTCCGACATGGAATCCACAATGATAATTTCGGTACAACCTTGGTGTACCAATTCCCGCGCCTGTAAAAATTCAGGGATCACGCCGATAACGGTACCGCCGTTGGa                                                                                         >  1:26952/1‑251 (MQ=255)                                                                                                                                                aTCTTGGTTAAATAGAATACAAGGATGAGGATTTTTACCGATTCGCGCCCAAGAATAGACTTCGCTGATTTCTTCCAGCGTGCCCGGCCCACCCGGCAAAGCAATACAAGCATCGGCTAATGCCAGCATTTTGGCTTTTCGCTCCGACATGGAATCCACAATGATAATTTCGGTACAACCTTGGTGTACCAATTCCCGCGCCTGTAAAAATTCAGGGATCACGCCGATAACGGTACCGCCGTTGGAAAGTa                                                                                    >  1:316410/1‑251 (MQ=255)                                                                                                                                                      gTTAAATAGAATACAAGGATGAGGATTTTTACCGATTCGCGCCCAAGAATAGACTTCGCTGATTTCTTCCAGCGTGCCCGGCCCACCCGGCAAAGCAATACAAGCATCGGCTAATGCCAGCATTTTGGCTTTTCGCTCCGACATGGAATCCACAATGATAATTTCGGTACAACCTTGGTGTACCAATTCCCGCGCCTGTAAAAATTCAGGGATCACGCCGATAACGGTACCGCCGTTGGAAAGTACCGTAt                                                                              >  1:302542/1‑251 (MQ=255)                                                                                                                                                      gTTAAATAGAATACAAGGATGAGGATTTTTACCGATTCGCGCCCAAGAATAGACTTCGCTGATTTCTTCCAGCGTGCCCGGCCCACCCGGCAAAGCAATACAAGCATCGGCTAATGCCAGCATTTTGGCTTTTCGCTCCGACATGGAATCCACAATGATAATTTCGGTACAACCTTGGTGTACCAATTCCCGCGCCTGT‑AAAATTCAGGGATCACGCCGATAACGGTAACCCCGGTGGGAAATACCGTaa                                                                              >  1:449610/1‑249 (MQ=255)                                                                                                                                                           aTAGAATACAAGGATGAGGATTTTTACCGATTCGCGCCCAAGAATAGCCTTCGCTGATTTCTTCCAGCGTGCCCGGCCCACCCGGCAAAGCAATACAAGCATCGGCTAATGCCAGCATTTTGGCTTTTCGCTCCGACATGGAATCCACAATGATAATTTCGGTACAACCTTGGTGTACCAATTCCCGCGCCTGTAAAAATTCAGGGATCACGCCGATAACGGTAccgc                                                                                                <  1:397551/228‑1 (MQ=255)                                                                                                                                                           aTAGAATACAAGGATGAGGATTTTTACCGATTCGCGCCCAAGAATAGACTTCGCTGATTTCTTCCAGCGTGCCCGGCCCACCCGGCAAAGCAATACAAGCATCGGCTAATGCCAGCATTTTGGCTTTTCGCTCCGACATGGAATCCACAATGATAATTTCGGTACAACCTTGGTGTACCAATTCCCGCGCCTGTAAAAATTCAGGGATCACGCCGATAACGGt                                                                                                     <  1:528854/223‑1 (MQ=255)                                                                                                                                                           aTAGAATACAAGGATGAGGATTTTTACCGATTCGCGCCCAAGAATAGACTTCGCTGATTTCTTCCAGCGTGCCCGGCCCACCCGGCAAAGCAATACAAGCATCGGCTAATGCCAGCATTTTGGCTTTTCGCTCCGACATGGAATCCACAATGATAATTTCGGTACAACCTTGGTGTACCAATTCCCGCGCCTGTAAAAATTCAGGGATCACGCCGATAACGGt                                                                                                     >  1:773467/1‑223 (MQ=255)                                                                                                                                                           aTAGAATACAAGGATGAGGATTTTTACCGATTCGCGCCCAAGAATAGACTTCGCTGATTTCTTCCAGCGTGCCCGGCCCACCCGGCAAAGCAATACAAGCATCGGCTAATGCCAGCATTTTGGCTTTTCGCTCCGACATGGAATCCACAATGATAATTTCGGTACAACCTTGGTGTACCAATTCCCGCGCCTGTAAAAATTCAGGGATCACGCCGATAACGGTACCGCCGTTGGAAAGTACCGTATCCGc                                                                          >  1:653822/1‑250 (MQ=255)                                                                                                                                                           aTAGAATACAAGGATGAGGATTTTTACCGATTCGCGCCCAAGAATAGACTTCGCTGATTTCTTCCAGCGTGCCCGGCCCACCCGGCAAAGCAATACAAGCATCGGCTAATGCCAGCATTTTGGCTTTTCGCTCCGACATGGAATCCACAATGATAATTTCGGTACAACCTTGGTGTACCAATTCCCGCGCCTGTAAAAATTCAGGGATCACGCCGATAACGGTACCGCCGTTGGAAAGTACCGTATCCGc                                                                          >  1:104811/1‑250 (MQ=255)                                                                                                                                                              gAATACAAGGATGAGGATTTTTCCCGATCCGCGCCCAAGAATAGACTTCGCTGATTTCTTCCAGCGTCCCCGGCCCCCCCGGCAAAGCAATACAAGCATCGGCTAATGCCAGCATTTTGGTTTTTCGCTCCGACATGGAACCCACAATGATAATTTCGGTACAACCTTGGTGTACCAATCCCCGCGCCTGTAAAAATTCAGGGATCACGCCGATAACGGTACCGCCGTTGGaa                                                                                        <  1:166818/233‑1 (MQ=255)                                                                                                                                                              gAATACAAGGATGAGGATTTTTACCGATTCGCGCCCAAGAATAGACTTCGCTGATTTCTTCCAGCGTGCCCGGCCCACCCGGCAAAGCAATACAAGCATCGGCTAATGCCAGCATTTTGGCTTTTCGCTCCGACATGGAATCCACAATGATAATTTCGGTACAACCTTGgtg                                                                                                                                                     <  1:171909/172‑1 (MQ=255)                                                                                                                                                              gAATACAAGGATGAGGATTTTTACCGATTCGCGCCCAAGAATAGACTTCGCTGATTTCTTCCAGCGTGCCCGGCCCACCCGGCAAAGCAATACAAGCATCGGCTAATGCCAGCATTTTGGCTTTTCGCTCCGACATGGAATCCACAATGATAATTTCGGTACAACCTTGGTGTACCAATTCCCGCGCCTGTAAAAATTCAGGGATCACGCCGATAACGGTACCGCCGTTGGAAAGTACCGTATCCGCCaa                                                                       >  1:522005/1‑250 (MQ=255)                                                                                                                                                              gAATACAAGGATGAGGATTTTTACCGATTCGCGCCCAAGAATAGACTTCGCTGATTTCTTCCAGCGTGCCCGGCCCACCCGGCAAAGCAATACAAGCATCGGCTAATGCCAGCATTTTGGCTTTTCGCTCCGACATGGAATCCACAATGATAATTTCGGTACAACCTTGGTGTACCAATTCCCGCGCCTGTAAAAATTCAGGGATCACGCCGATAACGGTACCGCCGTTGGAAAGTACCGTATCCGCCaa                                                                       >  1:366293/1‑250 (MQ=255)                                                                                                                                                                aTACAAGGATGAGGATTTTTACCGATTCGCGCCCAAGAATAGACTTCGCTGATTTCTTCCAGCGTGCCCGGCCCACCCGGCAAAGCAATACAAGCATCGGCTAATGCCAGCATTTTGGCTTTTCGCTCCGACATGGAATCCACAATGATAATTTCGGTACAACCTTGGTGTACCAATTCCCGCGCCTGTAAAAATTCAGGGATCACGCCGATAACGGTGccgc                                                                                                <  1:337436/223‑1 (MQ=255)                                                                                                                                                                aTACAAGGATGAGGATTTTTACCGATTCGCGCCCAAGAATAGACTTCGCTGATTTCTTCCAGCGTGCCCGGCCCACCCGGCAAAGCAATACAAGCATCGGCTAATGCCAGCATTTTGGCTTTTCGCTCCGACATGGAATCCACAATGATAATTTCGGTACAACCTTGGTGTACCAATTCCCGCGCCTGTAAAAATTCAGGGATCACGCCGATAACGGTACCGCCGTTGGAAAGTACCGTAt                                                                              >  1:569988/1‑241 (MQ=255)                                                                                                                                                                aTACAAGGATGAGGATTTTTACCGATTCGCGCCCAAGAATAGACTTCGCTGATTTCTTCCAGCGTGCCCGGCCCACCCGGCAAAGCAATACAAGCATCGGCTAATGCCAGCATTTTGGCTTTTCGCTCCGACATGGAATCCACAATGATAATTTCGGTACAACCTTGGTGTACCAATTCCCGCGCCTGTAAAAATTCAGGGATCACGCCGATAACGGTACCGCCGTTGGAAAGTACCGTATCCGCCAaca                                                                     >  1:557874/1‑250 (MQ=255)                                                                                                                                                                aTACAAGGATGAGGATTTTTACCGATTCGCGCCCAAGAATAGACTTCGCTGATTTCTTCCAGCGTGCCCGGCCCACCCGGCAAAGCAATACAAGCATCGGCTAATGCCAGCATTTTGGCTTTTCGCTCCGACATGGAATCCACAATGATAATTTCGGTACAACCTTGGTGCACCAATTCCCGCGCCTGTAAAAATTCAGGGATCACGcc                                                                                                              >  1:816907/1‑209 (MQ=255)                                                                                                                                                                       gaggAGGATTTTTCCCGATCCGCCCCAAAAAATAGCTTTCGTTGTTTTCTTCCGGCGTGCCCGCCCCACCGGGAAAAGCAATACAAGCACCGGCTAATGCCAGCTTTTGGGTTTTTCGCTCCGACAGGGAATCCACAATGAAAATTGCGGTACACCCTTGGTGTCCCAATCCCCGCGCCTGAAAAAATTCAGGGATCACGCCGATAACGGTACCGCCGTTGGAAAGTACCGTATCCGCCAacac                                                                    <  1:823775/241‑1 (MQ=255)                                                                                                                                                                       gATGAGGATTTTTACCGATTCGCGCCCAAGAATAGACTTCGCTGATTTCTTCCAGCGTGCCCGGCCCACCCGGCAAAGCAATACAAGCATCGGCTAATGCCAGCATTTTGGCTTTTCGCTCCGACATGGAATCCACAATGATAATTTCGGTACAACCTTGGTGTACCAATTCCCGCGCCTGTAAAAATTCAGGGATCACGCCGATAACGGTACCGCCGTTGGAAAGTACCGTATCCGCCAACACGCCCAt                                                              >  1:889788/1‑250 (MQ=255)                                                                                                                                                                        aTGAGGATTTTTACCGATTCGCGCCCAAGAATAGACTTCGCTTATTTCTCCCAGCGTGCCGGGCCCCCCCGGCAAAGCAAAACAAGCATCGGCTAATGCCAGCATTTGGGCTTTTCGCCCCGACATGGAACCCACGAGGAAAATTTGGGTACAACCTGGGTTTACCAATTCCCGGGCCTGAAAAAATTCAGGGATCCCGCCGATAACGGTACCGCCGTTGGAAAGTACCGTATCCGCCAACACGCCCATc                                                             <  1:68189/250‑1 (MQ=255)                                                                                                                                                                            ggATTTTTACCGATTCGCGCCCAAGAATAGACTTCGCTGATTTCTTCCAGCGTGCCCGGCCCACCCGGCAAAGCAATACAAGCATCGGCTAATGCCAGCATTTTGGCTTTTCGCTCCGACATGGAATCCACAATGATAATTTCGGTACAACCTTGgtg                                                                                                                                                     >  1:306576/1‑158 (MQ=255)                                                                                                                                                                            ggATTTTTACCGATTCGCGCCCAAGAATAGACTTCGCTGATTTCTTCCAGCGTGCCCGGCCCACCCGGCAAAGCAATACAAGCATCGGCTAATGCCAGCATTTTGGCTTTTCGCTCCGACATGGAATCCACAATGATAATTTCGGTACAACCTTGGTGTACCAATTCCCGCGCCTGTAAAAAt                                                                                                                            <  1:708925/183‑1 (MQ=255)                                                                                                                                                                                ttttCCCGATTCGCGCCCAAGAATAGCCTCCGCTGATTTCTCCCAGCGTCCCCGCCCCCCCCGGCAAAGAAATACAAGCACCGGCAAATGCCAGCATTTTGGCTTTTCGCTCCGACATGGAACCCACAATGATAATTTCGGTACACCCTTGGTGTCCCAATTCCCGCGCCTGTAAAAATTCAGGGATCCCGCCGATAACGGTACCGCCGTTGGAAAGTACCGTATCCGCCAACACGCCCATCAATCCCGcc                                                    <  1:725669/251‑1 (MQ=255)                                                                                                                                                                                               cccAAGAATAGACTTCGCTGATTTCTTCCAGCGTGCCCGGCCCACCCGGCAAAGCAATACAAGCATCGGCTAATGCCAGCATTTTGGCTTTTCGCTCCGACa                                                                                                                                                                                          <  1:151754/102‑1 (MQ=255)                                                                                                                                                                                               cccAAGAATAGACTTCGCTGATTTCTTCCAGCGTGCCCGGCCCACCCGGCAAAGCAATACAAGCATCGGCTAATGCCAGCATTTTGGCTTTTCGCTCCGACATGGAATCCACAATGATAATTTCGGTACAACCTTGGTGTACCAATTCCCGCGCCTGTAAAAATTCAGGGATCACGCCGATAACGGTACCGCCGTTGGAAAGTACCGTATCCGCCAACACGCCCATCAATCCCGCCTTTCCGCCGCCATaa                                     >  1:649183/1‑251 (MQ=255)                                                                                                                                                                                                 cAAGAATAGACTTCGCTGATTTCTTCCAGCGTGCCCGGCCCACCCGGCAAAGCAATACAAGCATCGGCTAATGCCAGCATTTTGGCTTTTCGCTCCGACATGGAATCCACAATGATAATTTCGGTACAACCTTgg                                                                                                                                                       <  1:484958/135‑1 (MQ=255)                                                                                                                                                                                                 aaaaaaTAGACTCCGCTATTTTCTCCCAGCGTCCCCGCCCCCCCCGGAAAAGAAATAAAAGCATCGGCTAATGCAAGCTTTGTGGTTTTCCGCCCCGACAGGGAACCCAAAAGGAAAATTCCGGTACACCCTGGGTGTACCAATCCCCGCGCCTGAAAAAATTCAGGGACCACGCCGATAACGGTACCGCCGTTGGAAAGTACCGTATCCGCCAACACGCCCATCAATCCCGCCTTTCCGCCGCCATAAAc                                   <  1:455127/247‑1 (MQ=255)                                                                                                                                                                                                   aGAATAGACTTCGCTGATTTCTTCCAGCGTGCCCGGCCCACCCGGCAAAGCAATACAAGCATCGGCTAATGCCAGCATTTTGGCTTTTCGCTCCGACATGGAATCCACAATGATAATTTCGGt                                                                                                                                                                 >  1:340097/1‑123 (MQ=255)                                                                                                                                                                                                   aGAATAGACTTCGCTGATTTCTTCCAGCGTGCCCGGCCCACCCGGCAAAGCAATACAAGCATCGGCTAATGCCAGCATTTTGGCTTTTCGCTCCGACATGGAATCCACAATGATAATTTCGGTACAACCt                                                                                                                                                          <  1:599100/130‑1 (MQ=255)                                                                                                                                                                                                   aGAATAGACTTCGCTGATTTCTTCCAGCGTGCCCGGCCCACCCGGCAAAGCAATACAAGCATCGGCTAATGCCAGCATTTTGGCTTTTCGCTCCGACATGGAATCCACAATGATAATTTCGGTACAACCTTGGTGTACCAATTCCCGCGCCTGTAAAAATTCAGGGATCACGCCGATAACGGTACCGCCGTTGGAAAGTACCGTATCCGCCAACACGCCCATCAATCCCGCCTTTCCGCCGCCATAAACTa                                 >  1:276226/1‑251 (MQ=255)                                                                                                                                                                                                   aGAATAGACTTCGCTGATTTCTTCCAGCGTGCCCGGCCCACCCGGCAAAGCAATACAAGCATCGGCTAATGCCAGCATTTTGGCTTTTCGCTCCGACATGGAATCCACAATGATAATTTCGGTACAACCTTGGTGTACCAATTCCCGCGCCTGTAAAAATTCAGGGATCACGCCGATAACGGTACCGCCGTTGGAAAGTACCGTATCCGCCAACACGCCCATCAATCCCGCCTTTCCGCCGCCATAAACTa                                 >  1:6126/1‑251 (MQ=255)                                                                                                                                                                                                    gAATAGACTTCGCTGATTTCTTCCAGCGTGCCCGGCCCACCCGGCAAAGCAATACAAGCATCGGCTAATGCCAGCATTTTGGCTTTTCGCTCCGACATGGAATCCACAATGATAATTTCGGTACAACCTTGGTGTACCAATTCCCGCGCCTGTAAAAATTCAGGGATCACGCCGATAACGGTACCGCCGTTGGAAAGTACCGTATCCGCCAACACGCCCATCAATCCCGCCTTTCCGCCGCCATAAACTaa                                >  1:119559/1‑251 (MQ=255)                                                                                                                                                                                                      atagcctccGCTGATTTCTCCCGGCGGGCCCGGCCCCCCGGGCAAAGAAATACAGGCACCGGCTAATGCCAGCATTTTGGCTTTCCGCCCCGACATGAAACCCAAAATAATATTTTCGGAACACCCTGGGTGTCCCATTTCCCGCGCCTGAAAAAATTCAGGGATCACGCCGATAACGGTACCGCCGTTGGAAAGTACCGTATCCGCCAACACGCCCATCAATCCCGCCTTTCCGCCGCCATAAACTAACg                              <  1:874748/243‑1 (MQ=255)                                                                                                                                                                                                              cGCTGATTTCTTCCAGCGTGCCCGGCCCACCCGGCAAAGCAATACAAGCATCGGCTAATGCCAGCATTTTGGCTTTTCGCTCTGACATGGAATCCACAATGATAATTTCGGTACAACCTTGGTGTACCAATTCCCGCGCCTGTAAAAATTCAGGGATCACGCCGATAACGGTACCGCCGTTGGAAAGTACCGTATCCGCCAACACGCCCATCAATCCCGCCTTTCCGCCGCCATACACTAACGTATGTCGa                      >  1:415383/1‑251 (MQ=255)                                                                                                                                                                                                                ctgtttttttcCAGCGTGCCCGCCCCCCCGGGCAAACAAATAAAAGCATCGGCTAATGCCAGCATTTTGGCTTTTCGCTCCAACATGGAACCCACAATGATAATTTCGGTACACCCTTGGTGTACCATTCCCCGCGCCTGTAAAAATTCAGGGATCACGCCGATAACGGTACCGCCGTTGGAAAGTACCGTATCCGCCAACACGCCCATCAATCCCGCCTTTCCGCCGCCATAAACTAACGTATGTCGATg                    <  1:448512/243‑1 (MQ=255)                                                                                                                                                                                                                  gATTTCTTCCAGCGTGCCCGGCCCACCCGGCAAAGCAATACAAGCATCGGCTAATGCCAGCATTTTGGCTTTTCGCTCCGACATGGAATCCACAATGATAATTTCGGTACAACCTTGGTGTACCAATTCCCGCGCCTGTAAAAATTCAGGGATCACGCCGATAACGGTACCGCCGTTGGAAAGTAc                                                                                   >  1:561517/1‑186 (MQ=255)                                                                                                                                                                                                                  gATTTCTCCCAGCGTCCCCGGCCCCCCCGGAAAAGCAATACAAGCATCGGTTAATGCCAGCTTTTTGGTTTTTCGCTCCGACATGGAACCCAAAATGATAATTTCGGTACACCCTTGGTGTACCAATCCCCGCGCTTGTAAAAATTCAGGGATCACGCCGATAACGGTACCGCCGTTGGAAAGTACCGTATCCGCCAACACGCCCATCAAt                                                          <  1:348372/211‑1 (MQ=255)                                                                                                                                                                                                                   aTTTCTTCCAGCGTGCCCGGCCCACCCGGCAAAGCAATACAAGCATCGGCTAATGCCAGCATTTTGGCTTTTCGCTCCGACATGGAATCcaca                                                                                                                                                                               <  1:758911/93‑1 (MQ=255)                                                                                                                                                                                                                       cttcCAGCGTGCCCGGCCCACCCGGCAAAGCAATACAAGCATCGGCTAATGCCAGCATTTTGGCTTTTCGCTCCGACATGGAATCCACAATGATAATTTCGGTACAACCTTGGTGTACCAATTCCCGCGCCTGTAAAAATTCAGGGATCACGCCGATAACGGTACCGCCGTTGGAAAGTACCGTATCCGCCAACACGCCCATCAATCCCGCCTTTCCGCCGCCATAAACTAACGTATGt                         >  1:739367/1‑239 (MQ=255)                                                                                                                                                                                                                                tGCCCGGCCCACCCGGCAAAGCAATACAAGCATCGGCTAATGCCAGCATTTTGGCTTTTCGCTCCGACATGGAATCCACAATGATAATTTCGGTACAACCTTGGTGTACCAATTCCCGCGCCTGTAAAAATTCAGGGATCACGCCGATAACGGTACCGCCGTTGGAAAGTACCGTATCCGCCAac                                                                      >  1:148561/1‑185 (MQ=255)                                                                                                                                                                                                                                  cccGCCCCACCCGGAAAAGCAATACAAGCATGGGCAAAGGCCAGCATTTTGGCTTTTCCCGCCGACATGGAATCCACAATAAAATTTTCGGTACACCCTTGGTGTCCAAATCCCCGCGCCGGAAAAAATTCAGGGATCACGCCAATAACGGTACCGCCGTTGGAAAGTACCGTATCCGCCAACACGCCCATCAATCCCGCCTTTCCGCCGCCATAAACTAACGTATGTCGATGTTGCGTAATCCATTCGcc  <  1:751917/251‑1 (MQ=255)                                                                                                                                                                                                                                      gCCCACCCGGCAAAGCAATACAAGCATCGGCTAATGCCAGCATTTTGGCTTTTCGCTCCGACATGGAATCCACAATGATAATTTCGGTAc                                                                                                                                                               >  1:782352/1‑90 (MQ=255)                                                                                                                                                                                                                                      gCCCACCCGGCAAAGCAATACAAGCATCGGCTAATGCCAGCATTTTGGCTTTTCGCTCCGACATGGAATCCACAATGATAATTTCGGTACAACCTTGGTGTACCAATTCCCGCGCCTGTAAAAATTCAggg                                                                                                                      <  1:70850/131‑1 (MQ=255)                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                              ATCTCAGGCACTTGATAATCAAGAATAAAACGTTCTATTTCAAAAATATTTGTACTAAATAATAACTTATCAAAATGACTTTGCGTCAAGAAACCGGCAGTGACCATGCTTTGATATTGACTTCTCAGCGGTTCATAAAAACCATCTTGGTTAAATAGAATACAAGGATGAGGATTTTTACCGATTCGCGCCCAAGAATAGACTTCGCTGATTTCTTCCAGCGTGCCCGGCCCACCCGACAAAGCAATACAAGCATCGGCTAATGCCAGCATTTTGGCTTTTCGCTCCGACATGGAATCCACAATGATAATTTCGGTACAACCTTGGTGTACCAATTCCCGCGCCTGTAAAAATTCAGGGATCACGCCGATAACGGTACCGCCGTTGGAAAGTACCGTATCCGCCAACACGCCCATCAATCCCGCCTTTCCGCCGCCATAAACTAACGTATGTCGATGTTGCGTAATCCATTCGCC  >  1/2038358‑2038833

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑