Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	2,038,365	G→A	P181S (CCT→TCT)	unknown ←	Lysine decarboxylase family

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	2,038,365	0	G	A	100.0%	88.7 / NA	32	P181S (CCT→TCT)	unknown	Lysine decarboxylase family
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (14/18);  total (14/18)

TTTTCATTTTTCTGCAATCCTTATTTTTTAGTGAAAAATAATAATGCGCGCCATCTTACACAAAAAAATCCATAGAAGCAAACTTATTTTAAAATAAAAAATAAGATACCGAAAAAAGTAACAACTTAGATTGGGGTAATAAGAAAAATCATGTAATGTACGAAAAATCCACCGAGTTAGTTCTCATAGAAAAACAACTTTATTTTTGACCGCACTTTTTACACATCTTCATAAGTACGAATCTCAGGCACTTGATAATCAAGAATAAAACGTTCTATTTCAAAAATATTTGTACTAAATAATAACTTATCAAAATGACTTTGCGTCAAGAAACCGGCAGTGACCATGCTTTGATATTGACTTCTCAGCGGTTCATAAAAACCATCTTGGTTAAATAGAATACAAGGATGAGGATTTTTACCGATTCGCGCCCAAGAATAGACTTCGCTGATTTCTTCCAGCGTGCCCGGCCCACCCGACAAAGCAATACA  >  1/2038118‑2038608                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                    ttttCATTTTTCTGCAATCCTTATTTTTTAGTGAAAAATAATAATGCGCGCCATCTTACACAAAAAAATCCATAGAAGCAAACTTATTTTAAAATAAAAAATAAGATGCCGAAAAAAGTAACAACTTAGATTGGGGTAATAAGAAAAATCATGTAATGTACGAAAAATCCACCGAGTTAGTTCTCATAGAAAAACAACTTTATTTTTGACCGCACTTTTTACACATCTTCATAAGTACGAATCTCAGacac                                                                                                                                                                                                                                                  <  1:600621/251‑1 (MQ=255)                   ccTTATTTTTTAGTGAAAAATAATAATGCGCGCCATCTTACACAAAAAAATCCATAGAAGCAAACTTATTTTAAAATAAAAAATAAGATGCCGAAAAAAGTAACAACTTAGATTGGGGTAATAAGAAAAATCATGTAATGTACGAAAAATCCACCGAGTTAGTTCTCATAGAAAAACAACTTTATTTTTGACCGCACTTTTTACACATCTTCATAAGTACGAATCTCAGACACTTGATAAt                                                                                                                                                                                                                                          >  1:861486/1‑241 (MQ=255)                                      ataataatGCGCGCCATCTTACACAAAAAAATCCATAGAAGCAAACTTATTTTAAAATAAAAAATAAGATGCCGAAAAAAGTAACAACTTAGATTGGGGTAATAAGAAAAATCATGTAATGTACGAAAAATCCACCGAGTTAGTTCTCATAGAAAAACAACTTTATTTTTGACCGCACTTTTTACACATCTTCATAAGTACGAATCTCAGACACTTGATAATCa                                                                                                                                                                                                                                        <  1:713253/224‑1 (MQ=255)                                                   ccATCTTACACAAAAAAATCCATAGAAGCAAACTTATTTTAAAATAAAAAATAAGATGCCGAAAAAAGTAACAACTTAGATTGGGGTAATAAGAAAAATCATGTAATGTACGAAAAATCCACCGAGTTAGTTCTCATAGAAAAACAACTTTATTTTTGACCGCACTTTTTACACATCTTCATAAGTACGAATCTCAGACACTTGATAATCAAGAATAAAACGTTCTATTTCAAAAATATTTGTACTAaata                                                                                                                                                                                                >  1:556311/1‑251 (MQ=255)                                                     aTCTTACACAAAAAAATCCATAGAAGCAAACTTATTTTAAAATAAAAAATAAGATGCCGAAAAAAGTAACAACTTAGATTGGGGTAATAAGAAAAATCATGTAATGTACGAAAAATCCACCGAGTTAGTTCTCATAGAAAAACAACTTTATTTTTGACCGCACTTTTTACACATCTTCATAAGTACGAATCTCAGACACTTGATAATCAAGAATAAAACGTTCTATTTCAAAAATATTTGTACTAaataat                                                                                                                                                                                              >  1:460817/1‑251 (MQ=255)                                                        ttACACAAAAAAATCCATAGAAGCAAACTTATTTTAAAATAAAAAATAAGATGCCGAAAAAAGTAACAACTTAGATTGGGGTAATAAGAAAAATCATGTAATGTACGAAAAATCCACCGAGTTAGTTCTCATAGAAAAACAACTTTATTTTTGACCGCACTTTTTACACATCTTCATAAGTACGAATCTCAGACACTTGATAATCAAGAATAAAACGTTCTATTTCAAAAATATTTGTACTAAATAATAAc                                                                                                                                                                                           <  1:688774/251‑1 (MQ=255)                                                                                                 aaaaataaGATGCCGAAAAAAGTAACAACTTAGATTGGGGTAATAAGAAAAATCATGTAATGTACGAAAAATCCACCGAGTTAGTTCTCATAGAAAAACAACTTTATTTTTGACCGCACTTTTTACACATCTTCATAAGTACGAATCTCAGACACTTGATAATCAAGAATAAAACGTTCTATTTCAAAAATATTTGTACTAAAtaataa                                                                                                                                                                                            <  1:757287/209‑1 (MQ=255)                                                                                                            gccGAAAAAAGTAACAACTTAGATTGGGGTAATAAGAAAAATCATGTAATGTACGAAAAATCCACCGAGTTAGTTCTCATAGAAAAACAACTTTATTTTTGACCGCACTTTTTACACATCTTCATAAGTACGAATCTCAGACACTTGATAATCAAGAATAAAACGTTCt                                                                                                                                                                                                                         >  1:749545/2‑169 (MQ=255)                                                                                                                         acaacTTAGATTGGGGTAATAAGAAAAATCATGTAATGTACGAAAAATCCACCGAGTTAGTTCTCATAGAAAAACAACTTTATTTTTGACCGCACTTTTTACACATCTTCATAAGTACGAATCTCAGACACTTGATAATCAAGAATAAAACGTTCTATTTCAAAAATATTTGTACTAAATAATAACTTATCAAAATGACTTTGCGTCAAGAAACCGGCAGTGACCATGCTTTGATATTGACTTCTCAGCgg                                                                                                                          <  1:336994/251‑1 (MQ=255)                                                                                                                           aacTTAGATTGGGGTAATAAGAAAAATCATGTAATGTACGAAAAATCCACCGAGTTAGTTCTCATAGAAAAACAACTTTATTTTTGACCGCACTTTTTACACATCTTCATAAGTACGAATCTCAGACACTTGATAATCAAGAAt                                                                                                                                                                                                                                   <  1:463878/144‑1 (MQ=255)                                                                                                                            acTTAGATTGGGGTAATAAGAAAAATCATGTAATGTACGAAAAATCCACCGAGTTAGTTCTCATAGAAAAACAACTTTATTTTTGACCGCACTTTTTACACATCTTCATAAGTACGAATCTCAGACACTTGATAATCAAGAATAAAACGTTCTATTTCAAAAATATTTGTACTAAATAATAAc                                                                                                                                                                                           <  1:789273/183‑1 (MQ=255)                                                                                                                                        gTAATAAGAAAAATCATGTAATGTACGAAAAATCCACCGAGTTAGTTCTCATAGAAAAACAACTTTATTTTTGACCGCACTTTTTACACATCTTCATAAGTACGAATCTCAGACACTTGATAAt                                                                                                                                                                                                                                          <  1:621053/124‑1 (MQ=255)                                                                                                                                         taataaGAAAAATCATGTAATGTACGAAAAATCCACCGAGTTAGTTCTCATAGAAAAACAACTTTATTTTTGACCGCACTTTTTACACATCTTCATAAGTACGAATCTCAGACACTTGATAATCAAGAATAAAACGTTCTATTTCAAAAATATTTGTACTAAATAATAACTTATCAAAATGACTTTGCGTCAAGAAACCGGCAGTGACCATGCTTTGATATTGACTTCTCAGCGGTTCATAAAAACCATCt                                                                                                          <  1:785063/251‑1 (MQ=255)                                                                                                                                             aaGAAAAATCATGTAATGTACGAAAAATCCACCGAGTTAGTTCTCATAGAAAAACAACTTTATTTTTGACCGCACTTTTTACACATCTTCATAAGTACGAATCTCAGACACTTGATAATCAAGAATAAAACg                                                                                                                                                                                                                             <  1:26352/132‑1 (MQ=255)                                                                                                                                                  aaaTCATGTAATGTACGAAAAATCCACCGAGTTAGTTCTCATAGAAAAACAACTTTATTTTTGACCGCACTTTTTACACATCTTCATAAGTACGAATCTCAGACACTTGATAATCAAGAATAAAACGTTCTATTTCAAAAATATTTGTACTAAATAATAACTTATCAAAATGACTTTGCGTCAAGAAACCGGCAGTGACCATGCTTTGATATTGACTTCTCAGCGGTTCATAAAAACCATCTTGGTTAAAt                                                                                                 >  1:369495/1‑251 (MQ=255)                                                                                                                                                    aTCATGTAATGTACGAAAAATCCACCGAGTTAGTTCTCATAGAAAAACAACTTTATTTTTGACCGCACTTTTTACACATCTTCATAAGTACGAATCTCAGACACTTGATAATCAAGAATAAAACGTTCTATTTCAAAAATATTTGTACTAAATAATAACTTATCAAAATGACTTTGCGTCAAGAAACCGGCAGTGACCATGCTTTGATATTGACTTCTCAGCGGTTCATAAAAACCATCTTGGTTAAATAg                                                                                               >  1:816601/1‑251 (MQ=255)                                                                                                                                                       atgtaatgtaCGAAAAATCCACCGAGTTAGTTCTCATAGAAAAACAACTTTATTTTTGACCGCACTTTTTACACATCTTCATAAGTACGAATCTCAGACACTTGATAATCAAGAATAAAACGTTCTATTTCAAAAATATTTGTACTAAATAATAACTTATCAAAATGACTTTGCGTCAAGAAACCGGCAGTGACCATGCTTTGATATTGACTTCTCAGCgg                                                                                                                          <  1:384778/221‑1 (MQ=255)                                                                                                                                                         gtaatgtaCGAAAAATCCACCGAGTTAGTTCTCATAGAAAAACAACTTTATTTTTGACCGCACTTTTTACACATCTTCATAAGTACGAATCTCAGACACTTGATAATCAAGAATAAAACGTTCTATTTCAAAAATATTTGTACTAAATAATAACTTATCAAAATGACTTTGCGTCAAGAAACCGGCAGTGACCATGCTTTGATATTGAc                                                                                                                                    <  1:434534/209‑1 (MQ=255)                                                                                                                                                                              gAGTTAGTTCTCATAGAAAAACAACTTTATTTTTGACCGCACTTTTTACACATCTTCATAAGTACGAATCTCAGACACTTGATAATCAAGAATAAAAc                                                                                                                                                                                                                              <  1:465674/98‑1 (MQ=255)                                                                                                                                                                               agttagttCTCATAGAAAAACAACTTTATTTTTGACCGCACTTTTTACACATCTTCATAAGTACGAATCTCAGACACTTGATAATCAAGAATAAAACGTTCTATTTCAAAAATATTTGTACTAaat                                                                                                                                                                                                 >  1:580471/1‑126 (MQ=255)                                                                                                                                                                                    gttCTCATAGAAAAACAACTTTATTTTTGACCGCACTTTTTACACATCTTCATAAGTACGAATCTCAGACACTTGATAATCAAGAATAAAACGTTCTATTTCAAAAATATTTGTACTAAATAATAACTTATCAAAATGACTTTGCGTCAAGAAACCGGCAGTGACCATGCTTTGATATTGACTTCTCAGCgg                                                                                                                          >  1:708723/1‑192 (MQ=255)                                                                                                                                                                                       ctcATAGAAAAACAACTTTATTTTTGACCGCACTTTTTACACATCTTCATAAGTACGAATCTCAGACACTTGATAATCAAGAATAAAACGTTCTATTTCAAAAATATTTGTACTAaat                                                                                                                                                                                                 >  1:737880/1‑118 (MQ=255)                                                                                                                                                                                       ctcATAGAAAAACAACTTTATTTTTGACCGCACTTTTTACACATCTTCATAAGTACGAATCTCAGACACTTGATAATCAAGAATAAAACGTTCTATTTCAAAAATATTTGTACTAAATAATAACTTATCAAAATGACTTTGCGTCAAGAAACCGGCAGTGACCATGCTTTGATATTGACTTCTCAGCGGTTCATAAAAACCATCTTGGTTAAATAGAATACAAGGATGAGGATTTTTACCGATTCGCGcc                                                             >  1:801480/1‑250 (MQ=255)                                                                                                                                                                                           tAGAAAAACAACTTTATTTTTGACCGCACTTTTTACACATCTTCATAAGTACGAATCTCAGACACTTGATAATCAAGAATAAAACGTTCTATTTCAAAAATATTTGTACTAaat                                                                                                                                                                                                 <  1:116392/114‑1 (MQ=255)                                                                                                                                                                                                                 aCCGCACTTTTTACACATCTTCATAAGTACGAATCTCAGACACTTGATAATCAAGAATAAAACGTTCTATTTCAAAAATATTTGTACTAAATAATAACTTATCAAAATGACTTTGCGTCAAGAAACCGGCAGTGACCATGCTTTGATATTGACTTCTCAGCGGTTCATAAAAACCATCTTGGTTAAATAGAATACAAGGATGAGGATTTTTACCGATTCGCGCCCAAGAATAGACTTCGCTGATTTCTTcc                                  <  1:816968/251‑1 (MQ=255)                                                                                                                                                                                                                     cACTTTTTACACATCTTCATAAGTACGAATCTCAGACACTTGATAATCAAGAATAAAACGTTCTATTTCAAAAATATTTGTACTAAATAATAACTTATCAAAATGACTTTGCGTCAAGAAACCGGCAGTGACCATGCTTTGATATTGACTTCTCAGCGGTTCATAAAAACCATCTTGGTTAAATAGAATACAAGGa                                                                                     >  1:898640/1‑196 (MQ=255)                                                                                                                                                                                                                     cACTTTTTACACATCTTCATAAGTACGAATCTCAGACACTTGATAATCAAGAATAAAACGTTCTATTTCAAAAATATTTGTACTAAATAATAACTTATCAAAATGACTTTGCGTCAAGAAACCGGCAGTGACCATGCTTTGATATTGACTTCTCAGCGGTTCATAAAAACCATCTTGGTTAAATAGAATACAAGGATGAGGATTTTTACCGATTCGCGCCCAAGAATAGACTTCGCTGATTTCTTCCAGCg                              >  1:383232/1‑251 (MQ=255)                                                                                                                                                                                                                       cTTTTTACACATCTTCATAAGTACGAATCTCAGACACTTGATAATCAAGAATAAAACGTTCTATTTCAAAAATATTTGTACTAAATAATAACTTATCAAAATGACTTTGCGTCAAGAAACCGGCAGTGACCATGCTTTGATATTGACTTCTCAGCGGTTCATAAAAACCATCTTGGTTAAATAGAATACAAGGATGAGGATTTTTACCGATTCGCGCCCAAGAATAg                                                    <  1:698775/227‑1 (MQ=255)                                                                                                                                                                                                                         ttttACACATCTTCATAAGTACGAATCTCAGACACTTGATAATCAAGAATAAAACGTTCTATTTCAAAAATATTTGTACTAAATAATAACTTATCAAAATGACTTTGCGTCAAGAAACCGGCAGTGACCATGCTTTGATATTGACTTCTCAGCGGTTCATAAAAACCATCTTGGTTAAATAGAATACAAGGa                                                                                     <  1:392840/192‑1 (MQ=255)                                                                                                                                                                                                                                      cATAAGTACGAATCTCAGACACTTGATAATCAAGAATAAAACGTTCTATTTCAAAAATATTTGTACTAAATAATAACTTATCAAAATGACTTTGCGTCAAGAAACCGGCAGTGACCATGCTTTGATATTGACTTCTCAGCGGTTCATAAAAACCATCTTGGTTAAATAGAATACAAGGATGAGGATTTTTACCGATTCGCGCCCAAGAATAGACTTCGCTGATTTCTTCCAGCGTGCCCGGCCCACCCgg              >  1:237947/1‑249 (MQ=255)                                                                                                                                                                                                                                           gTACGAATCTCAGACACTTGATAATCAAGAATAAAACGTTCTATTTCAAAAATATTTGTACTAAATAATAACTTATCAAAATGACTTTGCGTCAAGAAACCGGc                                                                                                                                                           <  1:385305/104‑1 (MQ=255)                                                                                                                                                                                                                                                 aTCTCAGACACTTGATAATCAAGAATAAAACGTTCTATTTCAAAAATATTTGTACTAAATAATAACTTATCAAAATGACTTTGCGTCAAGAAACCGGCAGTGACCATGCTTTGATATTGACTTCTCAGCGGTTCATAAAAACCATCTTGGTTAAATAGAATACAAGGa                                                                                     <  1:703526/168‑1 (MQ=255)                                                                                                                                                                                                                                                 aTCTCAGACACTTGATAATCAAGAATAAAACGTTCTATTTCAAAAATATTTGTACTAAATAATAACTTATCAAAATGACTTTGCGTCAAGAAACCGGCAGTGACCATGCTTTGATATTGACTTCTCAGCGGTTCATAAAAACCATCTTGGTTAAATAGAATACAAGGATGAGGATTTTTACCGATTCGCGCCCAAGAATAGACTTCGCTGATTTCTTCCAGCGTGCCCGGCCCACCCGGCAAAGCAATACa  >  1:698375/1‑251 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                    TTTTCATTTTTCTGCAATCCTTATTTTTTAGTGAAAAATAATAATGCGCGCCATCTTACACAAAAAAATCCATAGAAGCAAACTTATTTTAAAATAAAAAATAAGATACCGAAAAAAGTAACAACTTAGATTGGGGTAATAAGAAAAATCATGTAATGTACGAAAAATCCACCGAGTTAGTTCTCATAGAAAAACAACTTTATTTTTGACCGCACTTTTTACACATCTTCATAAGTACGAATCTCAGGCACTTGATAATCAAGAATAAAACGTTCTATTTCAAAAATATTTGTACTAAATAATAACTTATCAAAATGACTTTGCGTCAAGAAACCGGCAGTGACCATGCTTTGATATTGACTTCTCAGCGGTTCATAAAAACCATCTTGGTTAAATAGAATACAAGGATGAGGATTTTTACCGATTCGCGCCCAAGAATAGACTTCGCTGATTTCTTCCAGCGTGCCCGGCCCACCCGACAAAGCAATACA  >  1/2038118‑2038608

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑