Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	2,038,225	A→G	intergenic (‑101/+111)	unknown ← / ← unknown	LSU ribosomal protein L31p @ LSU ribosomal protein L31p, zinc‑independent/Lysine decarboxylase family

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	2,038,225	0	A	G	100.0%	91.6 / NA	32	intergenic (‑101/+111)	unknown/unknown	LSU ribosomal protein L31p @ LSU ribosomal protein L31p, zinc‑independent/Lysine decarboxylase family
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (12/20);  total (12/20)

GACCGGATATTCTTTTCCATCTTCCCATTTCATAGTGTTGGTGGTTCTGGCGCAGGAACGAATCAAAAAGCCTTGTTTGGCATTGGAGTCAT‑‑AAAAAAGAACGGTGCGATAATTTTCCGGATGAATACCTTTTTTCATTTTTCTGCAATCCTTATTTTTTAGTGAAAAATAATAATGCGCGCCATCTTACACAAAAAAATCCATAGAAGCAAACTTATTTTAAAATAAAAAATAAGATACCGAAAAAAGTAACAACTTAGATTGGGGTAATAAGAAAAATCATGTAATGTACGAAAAATCCACCGAGTTAGTTCTCATAGAAAAACAACTTTATTTTTGACCGCACTTTTTACACATCTTCATAAGTACGAATCTCAGGCACTTGATAATCAAGAATAAAACGTTCTATTTCAAAAATATTTGTACTAAATAATAAC  >  1/2037987‑2038423                                                                                                                                                                                                                                                 |                                                                                                                                                                                                       gACCGGATATTCTTTTCCATCTTCCCATTTCATAGTGTTGGTGGTTCTGGCGCAGGAACGAATCAAAAAGCCTTGTTTGGCATTGGAGTCAT‑‑AAAAAAGAACGGTGCGATAATTTTCCGGATGAATACCTTTTTTCATTTTTCTGCAATCCTTATTTTTTAGTGAAAAATAATAATGCGCGCCATCTTACACAAAAAAATCCATAGAAGCAAACTTATTTTAAAATAAAAAATAAGATGCCGAAAAAAGTa                                                                                                                                                                                            >  1:126847/1‑251 (MQ=255)   ccGGATATTCTTTTCCATCTTCCCATTTCATAGTGTTGGTGGTTCTGGCGCAGGAACGAATCAAAAAGCCTTGTTTGGCATTGGAGTCAT‑‑AAAAAAGAACGGTGCGATAATTTTCCGGATGAATACCTTTTTTCATTTTTCTGCAATCCTTATTTTTTAGTGAAAAATAATAATGCGCGCCATCTTACACAAAAAAATCCATAGAAGCAAACTTATTTTAAAATAAAAAATAAGATGCCGAAAAAAGTaac                                                                                                                                                                                          <  1:734108/251‑1 (MQ=255)         atTCTTTTCCATCTTCCCATTTCATAGTGTTGGTGGTTCTGGCGCAGGAACGAATCAAAAAGCCTTGTTTGGCATTGGAGTCATAAAAAAAAGAACGGTGCGATAATTTTCCGGATGAATACCTTTTTTCATTTTTCTGCAATCCTTATTTTTTAGTGAAAAATAATAATGCGCGCCATCTTACACAAAAAAATCCATAGAAGCAAACTTATTTTAAAATAAAAAATAAGATGCCGAAAAAAGTaacaa                                                                                                                                                                                        >  1:775488/1‑249 (MQ=255)             ttttCCATCTTCCCATTTCATAGTGTTGGTGGTTCTGGCGCAGGAACGAATCAAAAAGCCTTGTTTGGCATTGGAGTCAT‑‑AAAAAAGAACGGTGCGATAATTTTCCGGATGAATACCTTTTTTCATTTTTCTGCAATCCTTATTTTTTAGTGAAAAATAATAATGCGCGCCATCTTACACAAAAAAATCCATAGAAGCAAACTTATTTTAAAATAAAAAATAAGATGCCGAAAAAAGTAACAACTTAGAtt                                                                                                                                                                                <  1:157456/251‑1 (MQ=255)             ttttCCATCTTCCCATTTCATAGTGTTGGTGGTTCTGGCGCAGGAACGAATCAAAAAGCCTTGTTTGGCATTGGAGTCAT‑‑AAAAAAGAACGGTGCGATAATTTTCCGGATGAATACCTTTTTTCATTTTTCTGCAATCCTTATTTTTTAGTGAAAAATAATAATGCGCGCCATCTTACACAAAAAAATCCATAGAAGCAAACTTATTTTAAAATAAAAAATAAGATGCCGAAAAAAGTAACAACTTAGAtt                                                                                                                                                                                <  1:876671/251‑1 (MQ=255)                  cATCTTCCCATTTCATAGTGTTGGTGGTTCTGGCGCAGGAACGAATCAAAAAGCCTTGTTTGGCATTGGAGTCAT‑‑AAAAAAGAACGGTGCGATAATTTTCCGGATGAATACCTTTTTTCATTTTTCTGCAATCCTTATTTTTTAGTGAAAAATAATAATGCGCGCCATCTTACACAAAAAAATCCATAGAAGCAAACTTATTTTAAAATAAAAAATAAGATGCCGAAAAAAGTAACAACTTAGATTGGGGt                                                                                                                                                                           <  1:575704/251‑1 (MQ=255)                             ttCATAGTGTTGGTGGTTCTGGCGCAGGAACGAATCAAAAAGCCTTGTTTGGCATTGGAGTCAT‑‑AAAAAAGAACGGTGCGATAATTTTCCGGATGAATACCTTTTTTCATTTTTCTGCAATCCTTATTTTTTAGTGAAAAATAATAATGCGCGCCATCTTACACAAAAAAATCCATAGAAGCAAACTTATTTTAAAATAAAAAATAAGATGCCGAAAAAAGTAACAACTTAGATTGGGGTAATAAGaaaaa                                                                                                                                                                >  1:107050/1‑251 (MQ=255)                              tCATAGTGTTGGTGGTTCTGGCGCAGGAACGAATCAAAAAGCCTTGTTTGGCATTGGAGTCAT‑‑AAAAAAGAACGGTGCGATAATTTTCCGGATGAATACCTTTTTTCATTTTTCTGCAATCCTTATTTTTTAGTGAAAAATAATAATGCGCGCCATCTTACACAAAAAAATCCATAGAAGCAAACTTATTTTAAAATAAAAAATAAGATGCCGAAAAAAGTAACAACTTAGATTGGGGTAATAAGAAAAAt                                                                                                                                                               >  1:862637/1‑251 (MQ=255)                                             ttCTGGCGCAGGAACGAATCAAAAAGCCTTGTTTGGCATTGGAGTCAT‑‑AAAAAAGAACGGTGCGATAATTTTCCGGATGAATACCTTTTTTCATTTTTCTGCAATCCTTATTTTTTAGTGAAAAATAATAATGCGCGCCATCTTACACAAAAAAATCCATAGAAGCAAACTTATTTTAAAATAAAAAATAAGATGCCGAAAAAAGTAACAACTTAGATTGGGGTAATAAGAAAAATCATGTAATGTACGaa                                                                                                                                                <  1:23222/251‑1 (MQ=255)                                                                                gCATTGGAGTCAT‑‑AAAAAAGAACGGTGCGATAATTTTCCGGATGAATACCTTTTTTCATTTTTCTGCAATCCTTATTTTTTAGTGAAAAATAATAATGCGCGCCATCTTACACAAAAAAATCCATAGAAGCAAACTTATTTTAAAATAAAAAATAAGATGCCGAAAAAAGTAACAACTTAGATTGGGGTAATAAGAAAAATCATGTAATGTACGAAAAATCCACCGAGTTAGTTCTCATAGAAAaacaa                                                                                                               >  1:789829/1‑249 (MQ=255)                                                                                gCATGGGAGTCAT‑‑AAAAAAGAACGGTGCGATAATTTTCCGGATGAATACCTTTTTTCATTTTTCTGCAATCCTTATTTTTTAGTGAAAAATAATAATGCGCGCCATCTTACACAAAAAAATCCATAGAAGCAAACTTATTTTAAAATAAAAAATAAGATGCCGAAAAAAGTaac                                                                                                                                                                                          <  1:427045/174‑1 (MQ=255)                                                                                          cAT‑‑AAAAAAGAACGGTGCGATAATTTTCCGGATGAATACCTTTTTTCATTTTTCTGCAATCCTTATTTTTTAGTGAAAAATAATAATGCGCGCCATCTTACACAAAAAAATCCATAGAAGCAAACTTATTTTAAAATAAAAAATAAGATGCCGAAAAAAGTAACAACTTAGATTGGGGTAATAAGAAAAATCatgtaa                                                                                                                                                        <  1:61608/198‑1 (MQ=255)                                                                                           aT‑‑AAAAAAGAACGGTGCGATAATTTTCCGGATGAATACCTTTTTTCATTTTTCTGCAATCCTTATTTTTTAGTGAAAAATAATAATGCGCGCTATCTTACACAAAAAAATCCATAGAAGCAAACTTATTTTAAAATAAAAAATAAGATGCCGAAAAAAGTAACAACTTAGATTGGGGTAATAAGAAAAATCATGTAATGTACGAAAAATCCACCGAGTTAGTTCTCATAGAAAAACAACTTTATTTTTGAc                                                                                                  <  1:516531/251‑1 (MQ=255)                                                                                                           tGCGATAATTTTCCGGATGAATACCTTTTTTCATTTTTCTGCAATCCTTATTTTTTAGTGAAAAATAATAATGCGCGCCATCTTACACAAAAAAATCCATAGAAGCAAACTTATTTTAAAATAAAAAATAAGATGCCGAAAAAAGTAACAACTTAGATTGGGGTAATAAGAAAAATCATGTAATGTACGAAAAATCCACCGAGTTAGTTCTCATAGAAAAACAACTTTATTTTTGACCGCACTTTTTacac                                                                                    <  1:262791/251‑1 (MQ=255)                                                                                                            gCGATAATTTTCCGGATGAATACCTTTTTTCATTTTTCTGCAATCCTTATTTTTTAGTGAAAAATAATAATGCGCGCCATCTTACACAAAAAAATCCATAGAAGCAAACTTATTTTAAAATAAAAAATAAGATGCCGAAAAAAGTAACAACTTAGATTGGGGTAATAAGAAAAATCATGTAATGTACGAAAAATCCACCGAGTTAGTTCTCATAGAAAAACAACTTTATTTTTGACCGCACTTTTTacac                                                                                    >  1:92842/1‑250 (MQ=255)                                                                                                                                      ttttCATTTTTCTGCAATCCTTATTTTTTAGTGAAAAATAATAATGCGCGCCATCTTACACAAAAAAATCCATAGAAGCAAACTTATTTTAAAATAAAAAATAAGATGCCGAAAAAAGTAACAACTTAGATTGGGGTAATAAGAAAAATCATGTAATGTACGAAAAATCCACCGAGTTAGTTCTCATAGAAAAACAACTTTATTTTTGACCGCACTTTTTACACATCTTCATAAGTACGAATCTCAGacac                                                         <  1:600621/251‑1 (MQ=255)                                                                                                                                                        ccTTATTTTTTAGTGAAAAATAATAATGCGCGCCATCTTACACAAAAAAATCCATAGAAGCAAACTTATTTTAAAATAAAAAATAAGATGCCGAAAAAAGTAACAACTTAGATTGGGGTAATAAGAAAAATCATGTAATGTACGAAAAATCCACCGAGTTAGTTCTCATAGAAAAACAACTTTATTTTTGACCGCACTTTTTACACATCTTCATAAGTACGAATCTCAGACACTTGATAAt                                                 >  1:861486/1‑241 (MQ=255)                                                                                                                                                                           ataataatGCGCGCCATCTTACACAAAAAAATCCATAGAAGCAAACTTATTTTAAAATAAAAAATAAGATGCCGAAAAAAGTAACAACTTAGAtt                                                                                                                                                                                >  1:783271/1‑95 (MQ=255)                                                                                                                                                                           ataataatGCGCGCCATCTTACACAAAAAAATCCATAGAAGCAAACTTATTTTAAAATAAAAAATAAGATGCCGAAAAAAGTAACAACTTAGATTGGGGTAATAAGAAAAATCATGTAATGTACGAAAAATCCACCGAGTTAGTTCTCATAGAAAAACAACTTTATTTTTGACCGCACTTTTTac                                                                                      <  1:538940/185‑1 (MQ=255)                                                                                                                                                                           ataataatGCGCGCCATCTTACACAAAAAAATCCATAGAAGCAAACTTATTTTAAAATAAAAAATAAGATGCCGAAAAAAGTAACAACTTAGATTGGGGTAATAAGAAAAATCATGTAATGTACGAAAAATCCACCGAGTTAGTTCTCATAGAAAAACAACTTTATTTTTGACCGCACTTTTTACACATCTTCATAAGTACGAATCTCAgac                                                           >  1:559257/1‑210 (MQ=255)                                                                                                                                                                           ataataatGCGCGCCATCTTACACAAAAAAATCCATAGAAGCAAACTTATTTTAAAATAAAAAATAAGATGCCGAAAAAAGTAACAACTTAGATTGGGGTAATAAGAAAAATCATGTAATGTACGAAAAATCCACCGAGTTAGTTCTCATAGAAAAACAACTTTATTTTTGACCGCACTTTTTACACATCTTCATAAGTACGAATCTCAGACACTTGATAATCa                                               <  1:713253/224‑1 (MQ=255)                                                                                                                                                                                        ccATCTTACACAAAAAAATCCATAGAAGCAAACTTATTTTAAAATAAAAAATAAGATGCCGAAAAAAGTaacaa                                                                                                                                                                                        <  1:481320/74‑1 (MQ=255)                                                                                                                                                                                        ccATCTTACACAAAAAAATCCATAGAAGCAAACTTATTTTAAAATAAAAAATAAGATGCCGAAAAAAGTAACAACTTAGATTGGGGTAATAAGAAAAATCATGTAATGTACGAAAAATCCACCGAGTTAGTTCTCATAGAAAAACAACTTTATTTTTGACCGCACTTTTTACACATCTTCATAAGTACGAATCTCAGACACTTGATAATCAAGAATAAAACGTTCTATTTCAAAAATATTTGTACTAaata       >  1:556311/1‑251 (MQ=255)                                                                                                                                                                                          aTCTTACACAAAAAAATCCATAGAAGCAAACTTATTTTAAAATAAAAAATAAGATGCCGAAAAAAGTAACAACTTAGATTGGGGTAATAAGAAAAATCATGTAATGTACGAAAAATc                                                                                                                                           <  1:15923/117‑1 (MQ=255)                                                                                                                                                                                          aTCTTACACAAAAAAATCCATAGAAGCAAACTTATTTTAAAATAAAAAATAAGATGCCGAAAAAAGTAACAACTTAGATTGGGGTAATAAGAAAAATCATGTAATGTACGAAAAATCCACCGAGTTAGTTCTCATAGAAAAACAACTTTATTTTTGACCGCACTTTTTacac                                                                                    <  1:709352/172‑1 (MQ=255)                                                                                                                                                                                          aTCTTACACAAAAAAATCCATAGAAGCAAACTTATTTTAAAATAAAAAATAAGATGCCGAAAAAAGTAACAACTTAGATTGGGGTAATAAGAAAAATCATGTAATGTACGAAAAATCCACCGAGTTAGTTCTCATAGAAAAACAACTTTATTTTTGACCGCACTTTTTACACATCTTCATAAGTACGAAtc                                                                 <  1:277514/191‑1 (MQ=255)                                                                                                                                                                                          aTCTTACACAAAAAAATCCATAGAAGCAAACTTATTTTAAAATAAAAAATAAGATGCCGAAAAAAGTAACAACTTAGATTGGGGTAATAAGAAAAATCATGTAATGTACGAAAAATCCACCGAGTTAGTTCTCATAGAAAAACAACTTTATTTTTGACCGCACTTTTTACACATCTTCATAAGTACGAATCTCAGACACTTGATAATCAAGAATAAAACGTTCTATTTCAAAAATATTTGTACTAaataat     >  1:460817/1‑251 (MQ=255)                                                                                                                                                                                             ttACACAAAAAAATCCATAGAAGCAAACTTATTTTAAAATAAAAAATAAGATGCCGAAAAAAGTAACAACTTAGATTGGGGTAATAAGAAAAATCATGTAATGTACGAAAAATCCACCGAGTTAGTTCTCATAGAAAAACAACTTTATTTTTGACCGCACTTTTTACACATCTTCATAAGTACGAATCTCAGACACTTGATAATCAAGAATAAAACGTTCTATTTCAAAAATATTTGTACTAAATAATAAc  <  1:688774/251‑1 (MQ=255)                                                                                                                                                                                                                        cTTATTTTAAAATAAAAAATAAGATGCCGAAAAAAGTAACAACTTAGATTGGGGTAATAAGAAAAATCATGTAATGTACGAAAAATCCACCGAGTTAGTTCTCATAGAAAAACAACTTTATTTTTGACCGCACTTTTTACACATCTTCATaa                                                                          <  1:788578/152‑1 (MQ=255)                                                                                                                                                                                                                                      aaaaataaGATGCCGAAAAAAGTAACAACTTAGATTGGGGTAATAAGAAAAATCATGTAATGTACGAAAAATCCACCGAGTTAGTTCTCATAGAAAAACAACTTTATTTTTGACCGCACTTTTTACACATCTTCATAAGTACGAAtct                                                                >  1:628875/1‑148 (MQ=255)                                                                                                                                                                                                                                      aaaaataaGATGCCGAAAAAAGTAACAACTTAGATTGGGGTAATAAGAAAAATCATGTAATGTACGAAAAATCCACCGAGTTAGTTCTCATAGAAAAACAACTTTATTTTTGACCGCACTTTTTACACATCTTCATAAGTACGAATCTCAGACACTTGATAATCAAGAATAAAACGTTCTATTTCAAAAATATTTGTACTAAAtaataa   <  1:757287/209‑1 (MQ=255)                                                                                                                                                                                                                                        aaataaGATGCCGAAAAAAGTAACAACTTAGATTGGGGTAATAAGAAAAATCATGTAATGTACGAAAAATCCACCGAGTTAGTTCTCATAGAAAAACAACTTTATTTTTGAc                                                                                                  <  1:820228/112‑1 (MQ=255)                                                                                                                                                                                                                                                 |                                                                                                                                                                                                       GACCGGATATTCTTTTCCATCTTCCCATTTCATAGTGTTGGTGGTTCTGGCGCAGGAACGAATCAAAAAGCCTTGTTTGGCATTGGAGTCAT‑‑AAAAAAGAACGGTGCGATAATTTTCCGGATGAATACCTTTTTTCATTTTTCTGCAATCCTTATTTTTTAGTGAAAAATAATAATGCGCGCCATCTTACACAAAAAAATCCATAGAAGCAAACTTATTTTAAAATAAAAAATAAGATACCGAAAAAAGTAACAACTTAGATTGGGGTAATAAGAAAAATCATGTAATGTACGAAAAATCCACCGAGTTAGTTCTCATAGAAAAACAACTTTATTTTTGACCGCACTTTTTACACATCTTCATAAGTACGAATCTCAGGCACTTGATAATCAAGAATAAAACGTTCTATTTCAAAAATATTTGTACTAAATAATAAC  >  1/2037987‑2038423

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑