Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	2,033,808	C→T	L35L (CTG→TTG)	unknown →	Oligopeptide ABC transporter, permease protein OppC (TC 3.A.1.5.1)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	2,033,808	0	C	T	100.0%	186.1 / NA	65	L35L (CTG→TTG)	unknown	Oligopeptide ABC transporter, permease protein OppC (TC 3.A.1.5.1)
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (38/27);  total (38/27)

CTTAACCGCGATTATTCCCTTGTATTGAGCCTCACGATTTTGGTCGGCACACTGACTATCGTTTTTAACGCCGTAGTGGATATTTTATACGCCGTGATTGATCCGAAAATTCGTTATTAAGGAAGTAACATGACGAAAAATGATGGTGTAAAACCAATAAATCAAAAAAATACCGAATTTGTTGAACAACTTGCGGAACGCATGGATGAAATGCAACTGGAAGGGCGCAGCCTGTGGCAAGACGCCAAACGGCGTTTCTTCCGCAATAAAGCGGCGGTGGTCAGTTTAATCATGTTGGGTGTTATCGTGCTTTTCATTACTTTTGCGCCCCTCGCTTTCCCTTTTACTTATGAAGACACCGACTGGAACATGATGGGCGTGGCGCCGACGCTGGAAGGCTACCATTTCTTCGGTACGGATTCCTCCGGCCGCGATCTTTTGGTGCGTACCGCCATCGGCGGGCG  >  1/2033577‑2034040                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                         cTTAACCGCGATTATTCCCTTGTATTGAGCCTCACGATTTTGGTCGGCACACTGACTATCGTTTTTAACGCCGTAGTGGATATTTTATACGCCGTGATTGATCCGAAAATTCGTTATTAAGGAAGTAACATGACGAAAAATGATGGTGTAAAACCAATAAATCAAAAAAATACCGAATTTGTTGAACAACTTGCGGAACGCATGGATGAAATGCAACTGGAAGGGCGCAGCTTGTGGCAAGACGCCAAAc                                                                                                                                                                                                                        <  1:134955/250‑1 (MQ=255)  ttAACCGCGATTATTCCCTTGTATTGAGCCTCACGATTTTGGTCGGCACACTGACTATCGTTTTTAACGCCGTAGTGGATATTTTATACGCCGTGATTGATCCGAAAATTCGTTATTAAGGAAGTAACATGACGAAAAATGATGGTGTAAAACCAATAAATCAAAAAAATACCGAATTTGTTGAACAACTTGCGGAACGCATGGATGAAATGCAACTGGAAGGGCGCAGCTTGTGGCAAGACGCCAAACgg                                                                                                                                                                                                                      <  1:899860/251‑1 (MQ=255)           attattCCCTTGTATTGAGCCTCACGATTTTGGTCGGCACACTGACTATCGTTTTTAACGCCGTAGTGGATATTTTATACGCCGTGATTGATCCGAAAATTCGTTATTAAGGAAGTAACATGACGAAAAATGATGGTGTAAAACCAATAAATCAAAAAAATACCGAATTTGTTGAACAACTTGCGGAACGCATGGATGAAATGCAACTGGAAGGGCGCAGCTTGTGGCAAGACGCCAAACGGCGTttcttc                                                                                                                                                                                                             <  1:290570/251‑1 (MQ=255)            ttattCCCTTGTATTGAGCCTCACGATTTTGGTCGGCACACTGACTATCGTTTTTAACGCCGTAGTGGATATTTTATACGCCGTGATTGATCCGAAAATTCGTTATTAAGGAAGTAACATGACGAAAAATGATGGTGTAAAACCAATAAATCAAAAAAATACCGAATTTGTTGAACAACTTGCGGAACGCATGGATGAAATGCAACTGGAAGGGCGCAGCTTGTGGCAAGACGCCAAACGGCGTttcttc                                                                                                                                                                                                             >  1:734662/1‑250 (MQ=255)                 cccTTGTATTGAGCCTCACGATTTTGGTCGGCACACTGACTATCGTTTTTAACGCCGTAGTGGATATTTTATACGCCGTGATTGATCCGAAAATTCGTTATTAAGGAAGTAACATGACGAAAAATGATGGTGTAAAACCAATAAATCAAAAAAATACCGAATTTGTTGAACAACTTGCGGAACGCATGGATGAAATGCAACTGGAAGGGCGCAGCTTGTGGCAAGACGCCAAACGGCGTTTCTTCCGCAAt                                                                                                                                                                                                       >  1:126471/1‑251 (MQ=255)                   cTTGTATTGAGCCTCACGATTTTGGTCGGCACACTGACTATCGTTTTTAACGCCGTAGTGGATATTTTATACGCCGTGATTGATCCGAAAATTCGTTATTAAGGAAGTAACATGACGAAAAATGATGGTGTAAAGCCAATAAATCAAAAAAATACCGAATTTGTTGAACAACTTGCGGAACGCATGGATGAAATGCAACTGGAAGGGCGCAGCTTGTGGCAAGACGCCAAACGGCGTTTCTTCCGCAATaa                                                                                                                                                                                                     >  1:509846/1‑251 (MQ=255)                      gTATTGAGCCTCACGATTTTGGTCGGCACACTGACTATCGTTTTTAACGCCGTAGTGGATATTTTATACGCCGTGATTGATCCGAAAATTCGTTATTAAGGAAGTAACATGACGAAAAATGATGGTGTAAAACCAATAAATCAAAAAAATACCGAATTTGTTGAACAACTTGCGGAACGCATGGATGAAATGCAACTGGAAGGGCGCAGCTTGTGGCAAGACGCCAAACGGCGTTTCTTCCGCAATAAAg                                                                                                                                                                                                   >  1:261834/1‑250 (MQ=255)                      gTATTGAGCCTCACGATTTTGGTCGGCACACTGACTATCGTTTTTAACGCCGTAGTGGATATTTTATACGCCGTGATTGATCCGAAAATTCGTTATTAAGGAAGTAACATGACGAAAAATGATGGTGTAAAACCAATAAATCAAAAAAATACCGAATTTGTTGAACAACTTGCGGAACGCATGGATGAAATGCAACTGGAAGGGCGCAGCTTGTGGCAAGACGCCAAACGGCGTTTCTTCCGCAATAAAg                                                                                                                                                                                                   >  1:719654/1‑250 (MQ=255)                                     aTTTTGGTCGGCACACTGACTATCGTTTTTAACGCCGTAGTGGATATTTTATACGCCGTGATTGATCCGAAAATTCGTTATTAAGGAAGTAACATGACGAAAAATGATGGTGTAAAACCAATAAATCAAAAAAATACCGAATTTGTTGAACAACTTGCGGAACGCATGGATGAAATGCAACTGGAAGGGCGCAGCTTGTGGCAAGACGCCAAACGGCGTTTCTTCCGCAATAAAGCGGCGGTGGTCAGttt                                                                                                                                                                                   <  1:888914/251‑1 (MQ=255)                                           gTCGGCACACTGACTATCGTTTTTAACGCCGTAGTGGATATTTTATACGCCGTGATTGATCCGAAAATTCGTTATTAAGGAAGTAACATGACGAAAAATGATGGTGTAAAACCAATAAATCAAAAAAATACCGAATTTGTTGAACAACTTGCGGAACGCATGGATGAAATGCAACTGGAAGGGCGCAGCTTGTGGCAAGACGCCAAACGGCGTTTCTTCCGCAATAAAGCGGCGGTGGTCAGTTTAATCa                                                                                                                                                                              <  1:680074/250‑1 (MQ=255)                                               gCACACTGACTATCGTTTTTAACGCCGTAGTGGATATTTTATACGCCGTGATTGATCCGAAAATTCGTTATTAAGGAAGTAACATGACGAAAAATGATGGTGTAAAACCAATAAATCAAAAAAATACCGAATTTGTTGAACAACTTGCGGAACGCATGGATGAAATGCACCTGGAAGGGCGCAGCTTGTGGCAAGACGCCAAACGGCGTTTCTTCCGCAATAAAGCGGCGGTGGGCAGTTTAATCATGTTg                                                                                                                                                                         >  1:433515/1‑251 (MQ=255)                                                      gACTATCGTTTTTAACGCCGTAGTGGATATTTTATACGCCGTGATTGATCCGAAAATTCGTTATTAAGGAAGTAACATGACGAAAAATGATGGTGTAAAACCAATAAATCAAAAAAATACCGAATTTGTTGAACAACTTGCGGAACGCATGGATGAAATGCAACTGGAAGGGCGCAGCTTGTGGCAAGACGCCAAACGGCGTTTCTTCCGCAATAAAGCGGCGGTGGTCAGTTTAATCa                                                                                                                                                                              <  1:186655/239‑1 (MQ=255)                                                        cTATCGTTTTTAACGCCGTAGTGGATATTTTATACGCCGTGATTGATCCGAAAATTCGTTATTAAGGAAGTAACATGACGAAAAATGATGGTGTAAAACCAATAAATCAAAAAAATACCGAATTTGTTGAACAACTTGCGGAACGCATGGATGAAATGCAACTGGAAGGGCGCAGCTTGTGGCAAGACGCCAAACGGCGTTTCTTCCGCAATAAAGCGGCGGTGGTCAGTTTAATCATGTTGGGTGGTAt                                                                                                                                                                 >  1:710093/1‑250 (MQ=255)                                                        cTATCGTTTTTAACGCCGTAGTGGATATTTTATACGCCGTGATTGATCCGAAAATTCGTTATTAAGGAAGTAACATGACGAAAAATGATGGTGTAAAACCAATAAATCAAAAAAATACCGAATTTGTTGAACAACTTGCGGAACGCATGGATGAAATGCAACTGGAAGGGCGCAGCTTGTGGCAAGACGCCAAACGGCGTTTCTTCCGCAATAAAGCGGCGGGGGGCAGTTTAATCATGTTGGGTGTTAt                                                                                                                                                                 >  1:286272/1‑250 (MQ=255)                                                                           aGTGGATATTTTATACGCCGTGATTGATCCGAAAATTCGTTATTAAGGAAGTAACATGACGAAAAATGATGGTGTAAAACCAATAAATCAAAAAAATACCGAATTTGTTGAACAACTTGCGGAACGCATGGATGAAATGCAACTGGAAGGGCGCAGCTTGTGGCAAGACGCCAAACGGCGTTTCTTCCGCAATAAAGCGGCGGTGGTCATTTTAATCATGTTGGGTGTTATCGTGCTTTTCATTACTTTTg                                                                                                                                             >  1:572337/1‑251 (MQ=255)                                                                              ggATATTTTATACGCCGTGATTGATCCGAAAATTCGTTATTAAGGAAGTAACATGACGAAAAATGATGGTGTAAAACCAATAAATCAAAAAAATACCGAATTTGTTGAACAACTTGCGGAACGCATGGATGAAATGCAACTGGAAGGGCGCAGCTTGTGGCAAGACGCCAAACGGCGTTTCTTCCGCAATAAAGCGGCGGTGGTCAGTTTAATCATGTTGGGTGTTATCGTGCTTTTCATTACTTTTgcg                                                                                                                                           >  1:19431/1‑250 (MQ=255)                                                                               gATATTTTATACGCCGTGATTGATCCGAAAATTCGTTATTAAGGAAGTAACATGACGAAAAATGATGGTGTAAAACCAATAAATCAAAAAAATACCGAATTTGTTGAACAACTTGCGGAACGCATGGATGAAATGCAACTGGAAGGGCGCAGCTTGTGGCAAGACGCCAAACGGCGTTTCTTCCGCAATAAAGCGGCGGTGGTCAGTTTAATCATGTTGGGTGTTATCGTGCTTTTCATTACTTTTgcgc                                                                                                                                          <  1:215084/250‑1 (MQ=255)                                                                                  atTTTATACGCCGTGATTGATCCGAAAATTCGTTATTAAGGAAGTAACATGACGAAAAATGATGGTGTAAAACCAATAAATCAAAAAAATACCGAATTTGTTGAACAACTTGCGGAACGCATGGATGAAATGCAACTGGAAGGGCGCAGCTTGTGGCAAGACGCCAAACGGCGTTTCTTCCGCAATAAAGCGGCGGTGGTCAGTTTAATCATGTTGGGTGTTATCGTg                                                                                                                                                             >  1:106905/1‑228 (MQ=255)                                                                                  atTTTATACGCCGTGATTGATCCGAAAATTCGTTATTAAGGAAGTAACATGACGAAAAATGATGGTGTAAAACCAATAAATCAAAAAAATACCGAATTTGTTGAACAACTTGCGGAACGCATGGATGAAATGCAACTGGAAGGGCGCAGCTTGTGGCAAGACGCCAAACGGCGTTTCTTCCGCAATAAAGCGGCGGTGGTCAGTTTAATCATGTTGGGTGTTATCGTGc                                                                                                                                                            >  1:282196/1‑229 (MQ=255)                                                                                  atTTTATACGCCGTGATTGATCCGAAAATTCGTTATTAAGGAAGTAACATGACGAAAAATGATGGTGTAAAACCAATAAATCAAAAAAATACCGAATTTGTTGAACAACTTGCGGAACGCATGGATGAAATGCAACTGGAAGGGCGCAGCTTGTGGCAAGACGCCAAACGGCGTTTCTTCCGCAATAAAGCGGCGGTGGTCAGTTTAATCATGTTGGGTGTTATCGTGCTTTTCATTACTTTTgcgc                                                                                                                                          >  1:279985/1‑247 (MQ=255)                                                                                  atTTTATACGCCGTGATTGATCCGAAAATTCGTTATTAAGGAAGTAACATGACGAAAAATGATGGTGTAAAACCAATAAATCAAAAAAATACCGAATTTGTTGAACAACTTGCGGAACGCATGGATGAAATGCAACTGGAAGGGCGCAGCTTGTGGCAAGACGCCAAACGGCGTTTCTTCCGCAATAAAGCGGCGGTGGTCAGTTTAATCATGTTGGGTGTTATCGTGCTTTTCATTACTTTTGCGccc                                                                                                                                        >  1:129964/1‑249 (MQ=255)                                                                                  atTTTATACGCCGTGATTGATCCGAAAATTCGTTATTAAGGAAGTAACATGACGAAAAATGATGGTGTAAAACCAATAAATCAAAAAAATACCGAATTTGTTGAACAACTTGCGGAACGCATGGATGAAATGCAACTGGAAGGGCGCAGCTTGTGGCAAGACGCCAAACGGCGTTTCTTCCGCAATAAAGCGGCGGTGGTCAGTTTAATCATGTTGGGTGTTATCGTGCTTTTCATTACTTTTGCGccc                                                                                                                                        >  1:230760/1‑249 (MQ=255)                                                                                    tttATACGCCGTGATTGATCCGAAAATTCGTTATTAAGGAAGTAACATGACGAAAAATGATGGTGTAAAACCAATAAATCAAAAAAATACCGAATTTGTTGAACAACTTGCGGAACGCATGGATGAAATGCAACTGGAAGGGCGCAGCTTGTGGCAAGACGCCAAACGGCGTTTCTTCCGCAATAAAGCGGCGGTGGTCAGTTTAATCATGTTGGGGGTTATCGTGCTTTTCATTACTTTTGCGCCCCTCg                                                                                                                                    >  1:593138/1‑251 (MQ=255)                                                                                          cGCCGTGATTGATCCGAAAATTCGTTATTAAGGAAGTAACATGACGAAAAATGATGGTGTAAAACCAATAAATCAAAAAAATACCGAATTTGTTGAACAACTTGCGGAACGCATGGATGAAATGCAACTGGAAGGGCGCAGCTTGTGGCAAGACGCCAAACGGCGTTTCTTCCGCAATAAAGCGGCGGTGGGCAGTTTAATCATGt                                                                                                                                                                           >  1:190950/1‑206 (MQ=255)                                                                                            ccGTGATTGATCCGAAAATTCGTTATTAAGGAAGTAACATGACGAAAAATGATGGTGTAAAACCAATAAATCAAAAAAATACCGAATTTGTTGAACAACTTGCGGAACGCATGGATGAAATGCAACTGGAAGGGCGCAGCTTGTGGCAAGACGCCAAACgg                                                                                                                                                                                                                      >  1:874507/1‑161 (MQ=255)                                                                                            ccGTGATTGATCCGAAAATTCGTTATTAAGGAAGTAACATGACGAAAAATGATGGTGTAAAACCAATAAATCAAAAAAATACCGAATTTGTTGAACAACTTGCGGAACGCATGGATGAAATGCAACTGGAAGGGCGCAGCTTGTGGCAAGACGCCAAACGGCGTTTCTTCCGCAATAAAGCGGCGGTGGTCAGTTTAATCATGTTGGGTGTTATCGTGCTTTTCATTACTTTTGCGCCCCTCGCTTTccc                                                                                                                             <  1:457173/250‑1 (MQ=255)                                                                                            ccGTGATTGATCCGAAAATTCGTTATTAAGGAAGTAACATGACGAAAAATGATGGTGTAAAACCAATAAATCAAAAAAATACCGAATTTGTTGAACAACTTGCGGAACGCATGGATGAAATGCAACTGGAAGGGCGCAGCTTGTGGCAAGACGCCAAACGGCGTTTCTTCCGCAATAAAGCGGCGGTGGTCAGTTTAATCATGTTGGGTGTTATCGTGCTTTTCATTACTTTTGCGCCCCTCGCTTTcc                                                                                                                              >  1:356492/1‑249 (MQ=255)                                                                                                gattgatCCGAAAATTCGTTATTAAGGAAGTAACATGACGAAAAATGATGGTGTAAAACCAATAAATCAAAAAAATACCGAATTTGTTGAACAACTTGCGGAACGCATGGATGAAATGCAACTGGAAGGGCGCAGCTTGTGGCAAGACGCCAAACGGCGTTTCTTCCGCAATAAAGCGGCGGTGGTCAGTTTAATCATGTTGGGTGTTac                                                                                                                                                                 <  1:884953/210‑2 (MQ=255)                                                                                                gattgatCCGAAAATTCGTTATTAAGGAAGTAACATGACGAAAAATGATGGTGTAAAACCAATAAATCAAAAAAATACCGAATTTGTTGAACAACTTGCGGAACGCATGGATGAAATGCAACTGGAAGGGCGCAGCTTGTGGCAAGACGCCAAACGGCGTTTCTTCCGCAATAAAGCGGCGGTGGTCAGTTTAATCATGTTGGGTGTTAt                                                                                                                                                                 <  1:884970/210‑1 (MQ=255)                                                                                                 attgatCCGAAAATTCGTTATTAAGGAAGTAACATGACGAAAAATGATGGTGTAAAACCAATAAATCAAAAAAATACCGAATTTGTTGAACAACTTGCGGAACGCATGGATGAAATGCAACTGGAAGGGCGCAGCTTGTGGCAAGACGCCAAACGGCGTTTCTTCCGCAATAAAGCGGCGGTGGTCAGTTTAATCATGTTGGGTGTTATTGTGCTTTTCATTACTTTTGCGCCCCTCGCTTTCCCTTTTAc                                                                                                                       <  1:786582/251‑1 (MQ=255)                                                                                                 attgatCCGAAAATTCGTTATTAAGGAAGTAACATGACGAAAAATGATGGTGTAAAACCAATAAATCAAAAAAATACCGAATTTGTTGAACAACTTGCGGAACGCATGGATGAAATGCAACTGGAAGGGCGCAGCTTGTGGCAAGACGCCAAACGGCGTTTCTTCCGCAATAAAGCGGCGGTGGTCAGTTTAATCATGTTGGGTGTTATCGTGCTTTTCATTACTTTTGCGCCCCTCGCTTTCCCTTTTAc                                                                                                                       <  1:741241/251‑1 (MQ=255)                                                                                                             aTTCGTTATTAAGGAAGTAACATGACGAAAAATGATGGTGTAAAACCAATAAATCAAAAAAATACCGAATTTGTTGAACAACTTGCGGAACGCATGGATGAAATGCAACTGGAAGGGCGCAGCTTGTGGCAAGACGCCAAACGGCGTTTCTTCCGCAATAAAGCGGCGGTGGTCAGTTTAATCATGTTGGGTGTTATCGTGCTTTTCATTACTTTTGCGCCCCTCGCTTTCCCTTTTACTTATGAAGacac                                                                                                           <  1:597461/251‑1 (MQ=255)                                                                                                                  ttattaAGGAAGTAACATGACGAAAAATGATGGTGTAAAACCAATAAATCAAAAAAATACCGAATTTGTTGAACAACTTGCGGAACGCATGGATGAAATGCAACTGGAAGGGCGCAGCTTGTGGCAAGACGCCAAACGGCg                                                                                                                                                                                                                    <  1:288169/141‑1 (MQ=255)                                                                                                                  ttattaAGGAAGTAACATGACGAAAAATGATGGTGTAAAACCAATAAATCAAAAAAATACCGAATTTGTTGAACAACTTGCGGAACGCATGGATGAAATGCAACTGGAAGGGCGCAGCTTGTGGCAAGACGCCAAACGGCGTTTCTTCCGCAATAAAGCGGCGGTGGTCAGTTTAATCATGTTGGGTGTTATCGTGCTTTTCATTACTTTTGCGCCCCTCGCTTTCCCTTTTACTTATGAAg                                                                                                               <  1:807488/242‑1 (MQ=255)                                                                                                                    attaAGGAAGTAACATGACGAAAAATGATGGTGTAAAACCAATAAATCAAAAAAATACCGAATTTGTTGAACAACTTGCGGAACGCATGGATGAAATGCAACTGGAAGGGCGCAGCTTGTGGCAAGACGCCAAACGGCGTTTCTTCCGCAATAAAGCGGCGGTGGTCAGttt                                                                                                                                                                                   <  1:19761/172‑1 (MQ=255)                                                                                                                          gAAGTAACATGACGAAAAATGATGGTGTAAAACCAATAAATCAAAAAAATACCGAATTTGTTGAACAACTTGCGGAACGCATGGATGAAATGCAACTGGAAGGGCGCAGCTTGTGGCAAGACGCCAAACGGCGTTTCTTCCGCAATAAAGCGGCGGTGGTCAGTTTAATCATGTTGGGTGTTATCGTGCTTTTCATTACTTTTGCGCCCCTCGCTTTCCCTTTTACTTATGAAGACACCGACTGGAACatg                                                                                              >  1:225320/1‑251 (MQ=255)                                                                                                                           aaGTAACATGACGAAAAATGATGGTGTAAAACCAATAAATCAAAAAAATACCGAATTTGTTGAACAACTTGCGGAACGCATGGATGAAATGCAACTGGAAGGGCGCAGCTTGTGGCAAGACGCCAAACGGCGTTTCTTCCGCAATAAAGCGGCGGTGGTCAGTTTAATCATGTTGGGTGTTATCGTGCTTTTCATTACTTTTGCGCCCCTCGCTTTCCCTTTTACTTATGAAGACACCGACTGGAACatga                                                                                             <  1:567810/251‑1 (MQ=255)                                                                                                                              tAACATGACGAAAAATGATGGTGTAAAACCAATAAATCAAAAAAATACCGAATTTGTTGAACAACTTGCGGAACGCATGGATGAAATGCAACTGGAAGGGCGCAGCTTGTGGCAAGACGCCAAACGGCGTTTCTTCCGCAATAAAGCGGCGGTGGTCAGTTTAATCATGTTGGGTGTTATCGTGCTTTTCATTACTTTTGCGCCCCTCGCTTTCCCTTTTACTTATGAAGACACCGACTGGAACatgatg                                                                                           <  1:130435/250‑1 (MQ=255)                                                                                                                              tAACATGACGAAAAATGATGGTGTAAAACCAATAAATCAAAAAAATACCGAATTTGTTGAACAACTTGCGGAACGCATGGATGAAATGCAACTGGAAGGGCGCAGCTTGTGGCAAGACGCCAAACGGCGTTTCTTCCGCAATAAAGCGGCGGTGGTCAGTTTAATCATGTTGGGTGTTATCGTGCTTTTCATTACTTTTGCGCCCCTCGCTTTCCCTTTTACTTATGAAGACACCGACTGGAACatgat                                                                                            <  1:442575/249‑1 (MQ=255)                                                                                                                              tAACATGACGAAAAATGATGGTGTAAAACCAATAAATCAAAAAAATACCGAATTTGTTGAACAACTTGCGGAACGCATGGATGAAATGCAACTGG‑AGGGCGCAGCTTGTGGCAAGACGCCAAACGGCGTTTCTTCCGCAATAAAGCGGCGGTGGTCAGTTTAATCATGTTGGGTGTTATCGTGCTTTTCATTACTTTTGCGCCCCTCGCTTTCCCTTTTACTTATGAAGACACCGACTGGAACatgatg                                                                                           <  1:101641/249‑1 (MQ=255)                                                                                                                                        aaaaaTGATGGTGTAAAACCAATAAATCAAAAAAATACCGAATTTGTTGAACAACTTGCGGAACGCATGGATGAAATGCAACTGGAAGGGCGCAGCTTGTGGCAAGACGCCAAACGGCGTTTCTTCCGCAATAAAGCGGCGGTGGTCAGTTTAATCATGTTGGGTGTTATCGTGCTTTTCATTACTTTTGCGCCCCTCGCTTTCCCTTTTACTTATGAAGac                                                                                                             >  1:768229/1‑222 (MQ=255)                                                                                                                                            atgatgGTGTAAAACCAATAAATCAAAAAAATACCGAATTTGTTGAACAACTTGCGGAACGCATGGATGAAATGCAACTGGAAGGGCGCAGCTTGTGGCAAGa                                                                                                                                                                                                                                >  1:252934/1‑103 (MQ=255)                                                                                                                                                 ggTGTAAAACCAATAAATCAAAAAAATACCGAATTTGTTGAACAACTTGCGGAACGCATGGATGAAATGCAACTGGAAGGGCGCAGCTTGTGGCAAGACGCCAAACGGCGTTTCTTCCGCAATAAAGCGGCGGTGGTCAGTTTAATCATGTTGGGTGTTATCGTGCTTTTCATTACTTTTGCGCCCCTCGCTTTCCCTTTTACTTATGAAGACACCGACTGGAACatgat                                                                                            >  1:717408/1‑230 (MQ=255)                                                                                                                                                 ggTGTAAAACCAATAAATCAAAAAAATACCGAATTTGTTGAACAACTTGCGGAACGCATGGATGAAATGCAACTGGAAGGGCGCAGCTTGTGGCAAGACGCCAAACGGCGTTTCTTCCGCAATAAAGCGGCGGTGGTCAGTTTAATCATGTTGGGTGTTATCGTGCTTTTCATTACTTTTGCGCCCCTCGCTTTCCCTTTTACTTATGAAGACACCGACTGGAACATGATGGGCGTGGCGCCGACGCTGGa                                                                       >  1:50361/1‑251 (MQ=255)                                                                                                                                                  gtgtAAAACCAATAAATCAAAAAAATACCGAATTTGTTGAACAACTTGCGGAACGCATGGATGAAATGCAACTGGAAGGGCGCAGCTTGTGGCAAGACGCCAAACGGCGTTTCTTCCGCAATAAAGCGGCGGTGGTCAGTTTAATCATGTTGGGTGTTATCGTGCTTTTCATTACTTTTGCGCCCCTCGCTTTCCCTTTTACTTATGAAg                                                                                                               >  1:692240/1‑210 (MQ=255)                                                                                                                                                  gtgtAAAACCAATAAATCAAAAAAATACCGAATTTGTTGAACAACTTGCGGAACGCATGGATGAAATGCAACTGGAAGGGCGCAGCTTGTGGCAAGACGCCAAACGGCGTTTCTTCCGCAATAAAGCGGCGGTGGTCAGTTTAATCATGTTGGGTGTTATCGTGCTTTTCATTACTTTTGCGCCCCTCGCTTTCCCTTTTACTTATGAAGACACCGACTGGAACat                                                                                               >  1:438325/1‑226 (MQ=255)                                                                                                                                                          ccAATAAATCAAAAAAATACCGAATTTGTTGAACAACTTGCGGAACGCATGGATGAAATGCAACTGGAAGGGCGCAGCTTGTGGCAAGACGCCAAACGGCGTTTCTTCCGCAATAAAGCGGCGGTGGTCAGTTTAATCATGTTGGGTGTTATCGTGCTTTTCATTACTTTTGCGCCCCTCGCTTTCCCTTTTACTTATGAAGACACCGACTGGAACat                                                                                               >  1:352769/1‑218 (MQ=255)                                                                                                                                                          ccAATAAATCAAAAAAATACCGAATTTGTTGAACAACTTGCGGAACGCATGGATGAAATGCAACTGGAAGGGCGCAGCTTGTGGCAAGACGCCAAACGGCGTTTCTTCCGCAATAAAGCGGCGGTGGTCAGTTTAATCATGTTGGGTGTTATCGTGCTTTTCATTACTTTTGCGCCCCTCGCTTTCCCTTTTACTTATGAAGACACCGACTGGAACATGATGGGCGTGGCGCCGACGCTGGAAGGCTACCa                                                              <  1:629308/251‑1 (MQ=255)                                                                                                                                                            aaTAAATCAAAAAAATACCGAATTTGTTGAACAACTTGCGGAACGCATGGATGAAATGCAACTGGAAGGGCGCAGCTTGTGGCAAGACGCCAAACGGCGTTTCTTCCGCAATAAAGCGGCGGTGGTCAGTTTAATCATGTTGGGTGTTATCGTGCTTTTCATTACTTTTGCGCCCCTCGCTTTCCCTTTTACTTATGAAGACACCGACTGGAACATGATGGGCGTGGCGCCGACGCTGGAAGGCTACCAtt                                                            <  1:174667/251‑1 (MQ=255)                                                                                                                                                            aaTAAATCAAAAAAATACCGAATTTGTTGAACAACTTGCGGAACGCATGGATGAAATGCAACTGGAAGGGCGCAGCTTGTGGCAAGACGCCAAACGGCGTTTCTTCCGCAATAAAGCGGCGGTGGTCAGTTTAATCATGTTGGGTGTTATCGTGCTTTTCATTACTTTTGCGCCCCTCGCTTTCCCTTTTACTTATGAAGACACCGACTGGAACATGATGGGCGTGGCGCCGACGCTGGAAGGCTACCAtt                                                            <  1:828564/251‑1 (MQ=255)                                                                                                                                                                     aaaaaaTACCGAATTTGTTGAACAACTTGCGGAACGCATGGATGAAATGCAACTGGAAGGGCGCAGCTTGTGGCAAGACGCCAAACGGCGTTTCTTCCGCAATAAAGCGGCGGTGGTCAGTTTAATc                                                                                                                                                                               >  1:891212/1‑127 (MQ=255)                                                                                                                                                                      aaaaaTGCCGAAATTGTTGAACAACTTGCGGAACGCATGGATGAAATGCAACTGGAAGGGCGCAGCTTGTGGCAAGACGCCAAACGGCGTTTCTTCCGCAATAAAGCGGCGGTGGTCAGTTTAATCATGTTGGGTGTTATCGTGCTTTTCATTACTTTTGCGCCCCTCGCTTTCCCTTTTACTTATGAAGACACCGACTGGAACATGATGGGCGTGGCGCCGACGCTGGAAGGCTACCATTTCTTCGGTAc                                                  <  1:752613/251‑1 (MQ=255)                                                                                                                                                                          aTACCGAATTTGTTGAACAACTTGCGGAACGCATGGATGAAATGCAACTGGAAGGGCGCAGCTTGTGGCAAGACGCCAAACGGCGTTTCTTCCGCAATAAAGCGGCGGTGGTCAGTTTAATCATGTTGGGTGTTATCGTGCTTTTCATTACTTTTGCGCCCCTCGCTTTCCCTTTTACTTATGAAGac                                                                                                             >  1:406581/1‑188 (MQ=255)                                                                                                                                                                                   ttgttgAACAACTTGCGGAACGCATGGATGAAATGCAACTGGAAGGGCGCAGCTTGTGGCAAGACGCCAAACGGCGTTTCTTCCGCAATAAAGCGGCGGTGGTCAGTTTAATCATGTTGGGTGTTATCGTGCTTTTCATTACTTTTGCGCCCCTCGCTTTCCCTTTTACTTATGAAGACACCGACTGGAACATGATGGGCGTGGCGCCGACGCTGGAAGGCTACCATTTCTTCGGTACGGATTCCTCCgg                                      >  1:783825/1‑250 (MQ=255)                                                                                                                                                                                            aacTTGCGGAACGCATGGATGAAATGCAACTGGAAGGGCGCAGCTTGTGGCAAGACGCCAAACGGCGTTTCTTCCGCAATAAAGCGGCGGTGGTCAGTTTAATCATGTTGGGTGTTATCGTGCTTTTCATTACTTTTGCGCCCCTCGCTTTCCCTTTTACTTATGAAGACACCGACTGGAACATGATGGGCGTGGCGCCGACGCTGGaa                                                                      >  1:187920/1‑209 (MQ=255)                                                                                                                                                                                              cTTGCGGAACGCATGGATGAAATGCAACTGGAAGGGCGCAGCTTGTGGCAAGACGCCAAACGGCGTTTCTTCCGCAATAAAGCGGCGGTGGTCAGTTTAATCATGTTGGGTGTTATCGTGCTTTTCATTACTTTTGCGCCCCTCGCTTTCCCTTTTACTTATGAAGACACCGACTGGAAc                                                                                                 >  1:335936/1‑180 (MQ=255)                                                                                                                                                                                              cTTGCGGAACGCATGGATGAAATGCAACTGGAAGGGCGCAGCTTGTGGCAAGACGCCAAACGGCGTTTCTTCCGCAATAAAGCGGCGGTGGTCAGTTTAATCATGTTGGGTGTTATCGTGCTTTTCATTACTTTTGCGCCCCTCGCTTTCCCTTTTACTTATGAAGACACCGACTGGAACATGATGGGCGTGGCGCCGACGCTGGAAGGCTACCATTTCTTCGGTACGGATTCCTCCGGCCGCGATCtttt                          >  1:240695/1‑251 (MQ=255)                                                                                                                                                                                                      aCGCATGGATGAAATGCAACTGGAAGGGCGCAGCTTGTGGCAAGACGCCAAACGGCGTTTCTTCCGCAATAAAGCGGCGGTGGTCAGTTTAATCATGTTGGGTGTTATCGTGCTTTTCATTACTTTTGCGCCCCTCGCTTTCCCTTTTACTTATGAAGACACCGACTGGAACATGATGGGCGTGGCGCCGACGCTGGAAGGCTACCATTTCTTCGGTACGGATTCCTCCGGCCGCGATCTTTTgg                        <  1:580704/245‑1 (MQ=255)                                                                                                                                                                                                             gATGAAATGCAACTGGAAGGGCGCAGCTTGTGGCAAGACGCCAAACGGCGTTTCTTCCGCAATAAAGCGGCGGTGGTCAGTTTAATCATGTTGGGTGTTATCGTGCTTTTCATTACTTTTGCGCCCCTCGCTTTCCCTTTTACTTATGAAGACACCGACTGGAACATGATGGGCGTGGCGCCGACGCTGGAAGGCTACCATTTCTTCGGTACGGATTCCTCCGGCCGCGATCTTTTGGTGCGTACCGCCAt           >  1:325899/1‑251 (MQ=255)                                                                                                                                                                                                             gATGAAATGCAACTGGAAGGGCGCAGCTTGTGGCAAGACGCCAAACGGCGTTTCTTCCGCAATAAAGCGGCGGTGGTCAGTTTAATCATGTTGGGTGTTATCGTGCTTTTCATTACTTTTGCGCCCCTCGCTTTCCCTTTTACTTATGAAGACACCGACTGGAACATGATGGGCGTGGCGCCGACGCTGGAAGGCTACCATTTCTTCGGTACGGATTCCTCCGGCCGCGATCTTTTGGTGCGTACCGCCAt           >  1:262134/1‑251 (MQ=255)                                                                                                                                                                                                             gATGAAATGCAACTGGAAGGGCGCAGCTTGTGGCAAGACGCCAAACGGCGTTTCTTCCGCAATAAAGCGGCGGTGGCCAGTTTAATCATGTTGGGTGTTATCGTGCTTTTCAt                                                                                                                                                     >  1:275276/1‑113 (MQ=255)                                                                                                                                                                                                                   aTGCAACTGGAAGGGCGCAGCTTGTGGCAAGACGCCAAACGGCGTTTCTTCCGCAATAAAGCGGCGGTGGTCAGTTTAATCATGTTGGGTGTTATCGTGCTTTTCATTACTTTTGCGCCCCTCGCTTTCCCTTTTACTTATGAAGACACCGACTGGAACATGATGGGCGTGGCGCCGACGCTGGAAGGCTACCATttcttc                                                       >  1:735901/1‑201 (MQ=255)                                                                                                                                                                                                                      cAACTGGAAGGGCGCAGCTTGTGGCAAGACGCCAAACGGCGTTTCTTCCGCAATAAAGCGGCGGTGGTCAGTTTAATCATGTTGGGTGTTATCGTGCTTTTCATTACTTTTGCGCCCCTCGCTTTCCCTTTTACTTATGAAGACACCGACTGGAACATGATGGGCGTGGCGCCGACGCTGGAAGGCTACCATTTCTTCGGTACGGATTCCTCCGGCCGCGCTCTTTTGGTGCGTACCGCCCTCggcggggg  >  1:458616/1‑249 (MQ=255)                                                                                                                                                                                                                       aaCTGGAAGGGCGCAGCTTGTGGCAAGACGCCAAACGGCGTTTCTTCCGCAATAAAGCGGCGGTGGTCAGTTTAATCATGTTGGGTGTTATCGTGCTTTTCATTACTTTTgcgc                                                                                                                                          <  1:459950/114‑1 (MQ=255)                                                                                                                                                                                                                         cTGGAAGGGCGCAGCTTGTGGCAAGACGCCAAACGGCGTTTCTTCCGCAATAAAGCGGCGGTGGTCAGTTTAATCATGTTGGGTGTTATCGTGCTTTTCATTACTTTTGCGCCCCTCGCTTTCCCTTTTACTTATGAAg                                                                                                               <  1:582385/139‑1 (MQ=255)                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                         CTTAACCGCGATTATTCCCTTGTATTGAGCCTCACGATTTTGGTCGGCACACTGACTATCGTTTTTAACGCCGTAGTGGATATTTTATACGCCGTGATTGATCCGAAAATTCGTTATTAAGGAAGTAACATGACGAAAAATGATGGTGTAAAACCAATAAATCAAAAAAATACCGAATTTGTTGAACAACTTGCGGAACGCATGGATGAAATGCAACTGGAAGGGCGCAGCCTGTGGCAAGACGCCAAACGGCGTTTCTTCCGCAATAAAGCGGCGGTGGTCAGTTTAATCATGTTGGGTGTTATCGTGCTTTTCATTACTTTTGCGCCCCTCGCTTTCCCTTTTACTTATGAAGACACCGACTGGAACATGATGGGCGTGGCGCCGACGCTGGAAGGCTACCATTTCTTCGGTACGGATTCCTCCGGCCGCGATCTTTTGGTGCGTACCGCCATCGGCGGGCG  >  1/2033577‑2034040

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑