Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	2,031,609	A→G	L188L (TTA→TTG)	unknown →	Oligopeptide ABC transporter, substrate‑binding protein OppA (TC 3.A.1.5.1)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	2,031,609	0	A	G	100.0%	214.4 / NA	76	L188L (TTA→TTG)	unknown	Oligopeptide ABC transporter, substrate‑binding protein OppA (TC 3.A.1.5.1)
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (42/34);  total (42/34)

CTATTACGGCAAATGATTTCGTCTTCGCCTGGCGTCGTTTAGTAACACCGGCAACCGCCTCACCTTACGCCAGTTTCCTGGATTATCTTCAAGTTGAAAACGCACAAGACATTATTGACGGCAAGAAAAAACCGGAAGAACTTGGCGTGGAAGCAAAAGACAATTACACTTTTGTCGTTCACACCACAAACCCGGTGCCTTATGCGGTGGGTTTGACGACCCACCAATCCTTGTTACCGTTACCGCAAAAAATCGTGGAAAAATTCGGTGACGATTGGGTGAAAAAAGACAATATCGTCGGTAACGGTGCCTATAAATTAAAAAATCACGTCATCAACGAAAAAATTGAATTTGAGCGCAACCCGCAATATTGGAATGACAAAGAAACGCAAATTAACAACGCCACCTTCTTAGCCATTGAGAATTCCAGCACCGACGTGGCACGTTATCGTGCAGGAGATTTGGACATCACCAGCCACGC  >  1/2031368‑2031848                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                cTATTACGGCAAATGATTTCGTCTTCGCCTGGCGTCGTTTAGTAACACCGGCAACCGCCTCACCTTACGCCAGTTTCCTGGATTATCTTCAAGTTGAAAACGCACAAGACATTATTGACGGCAAGAAAAAACCGGAAGAACTTGGCGTGGAAGCAAAAGACAATTACACTTTTGTCGTTCACACCACAAACCCGGTGCCTTATGCGGTGGGTTTGACGACCCACCAATCCTTGTTACCGTTGCCGCaaaaa                                                                                                                                                                                                                                        >  1:300965/1‑251 (MQ=255)         gCAAATGATTTCGTCTTCGCCTGGCGTCGTTTAGTAACACCGGCAACCGCCTCACCTTACGCCAGTTTCCTGGATTATCTTCAAGTTGAAAACGCACAAGACATTATTGACGGCAAGAAAAAACCGGAAGAACTTGGCGTGGAAGCAAAAGACAATTACACTTTTGTCGTTCACACCACAAACCCGGTGCCTTATGCGGTGGGTTTGACGACCCACCAATCCTTGTTACCGTTGCCGCAAAAAATCGTGGa                                                                                                                                                                                                                                >  1:878230/1‑251 (MQ=255)               gATTTCGTCTTCGCCTGGCGTCGTTTAGTAACACCGGCAACCGCCTCACCTTACGCCAGTTTCCTGGATTATCTTCAAGTTGAAAACGCACAAGACATTATTGACGGCAAGAAAAAACCGGAAGAACTTGGCGTGGAAGCAAAAGACAATTACACTTTTGTCGTTCACACCACAAACCCGGTGCCTTATGCGGTGGGTTTGACGACCCACCAATCCTTGTTACCGTTGCCGCAAAAAATCGTGGAAAAAtt                                                                                                                                                                                                                          >  1:670130/1‑251 (MQ=255)                aTTTCGTCTTCGCCTGGCGTCGTTTAGTAACACCGGCAACCGCCTCACCTTACGCCAGTTTCCTGGATTGTCTTCAAGTTGAAAACGCACAAGACATTATTGACGGCAAGAAAAAACCGGAAGAACTTGGCGTGGAAGCAAAAGACAATTACACTTTTGTCGTTCACACCACAAACCCGGTGCCTTATGCGGTGGGTTTGACGACCCACCAATCCTTGTTACCGTTGCCGCAAAAAATCGTGGAAAAAtt                                                                                                                                                                                                                          >  1:638925/1‑250 (MQ=255)                                        tAGTAACACCGGCAACCGCCTCACCTTACGCCAGTTTCCTGGATTATCTTCAAGTTGAAAACGCACAAGACATTATTGACGGCAAGAAAAAACCGGAAGAACTTGGCGTGGAAGCAAAAGACAATTACACTTTTGTCGTTCACACCACAAACCCGGTGCCTTATGCGGTGGGTTTGACGACCCACCAATCCTTGTTACCGTTGCCGCAAAAAATCGTGGAAAAATTCGGTGACGATTGGGTGAAAAAAGa                                                                                                                                                                                                  >  1:736274/1‑250 (MQ=255)                                           aaaccccGGCAACCGCCTCACCTTACGCCAGTTTCCTGGATTATCTTCAAGTTGAAAACGCACAAGACATTATTGACGGCAAGAAAAAACCGGAAGAACTTGGCGTGGAAGCAAAAGACAATTACACTTTTGTCGTTCACACCACAAACCCGGTGCCTTATGCGGTGGGTTTGACGACCCACCAATCCTTGTTACCGTTGCCGCAAAAAATCGTGGAAAAATTCGGTGACGATTGGGTGAAAAAAGACAat                                                                                                                                                                                              <  1:692093/246‑1 (MQ=255)                                               acCGGCAACCGCCTCACCTTACGCCAGTTTCCTGGATTATCTTCAAGTTGAAAACGCACAAGACATTATTGACGGCAAGAAAAAACCGGAAGAACTTGGCGTGGAAGCAAAAGACAATTACACTTTTGTCGTTCACACCACAAACCCGGTGCCTTATGCGGTGGGTTTGACGACCCACCAATCCTTGTTACCGTTGCCGCAAAAAATCGTGGAAAAATTCGGTGACGATTGGGTGAAAAAAGACAATAtcg                                                                                                                                                                                          >  1:155177/1‑251 (MQ=255)                                                          ccTCACCTTACGCCAGTTTCCTGGATTATCTTCAAGTTGAAAACGCACAAGACATTATTGACGGCAAGAAAAAACCGGAAGAACTTGGCGTGGAAGCAAAAGACAATTACACTTTTGTCGTTCACACCACAAACCCGGTGCCTTATGCGGTGGGTTTGACGACCCACCAATCCTTGTTACCGTTGCCGCAAAAAATCGTGGAAAAATTCGGTGACGATTGGGTGAAAAAAGACAatat                                                                                                                                                                                            <  1:660249/238‑1 (MQ=255)                                                             cACCTTACGCCAGTTTCCTGGATTATCTTCAAGTTGAAAACGCACAAGACATTATTGACGGCAAGAAAAAACCGGAAGAACTTGGCGTGGAAGCAAAAGACAATTACACTTTTGTCGTTCACACCACAAACCCGGTGCCTTATGCGGTGGGTTTGACGACCCACCAATCCTTGTTACCGTTGCCGCAAAAAATCGTGGAAAAATTCGGTGACGATTGGGTGAAAAAAGACAATATCGTCGGTAACGGTGcc                                                                                                                                                                            <  1:552546/251‑1 (MQ=255)                                                               ccTTACGCCAGTTTCCTGGATTATCTTCAAGTTGAAAACGCACAAGACATTATTGACGGCAAGAAAAAACCGGAAGAACTTGGCGTGGAAGCAAAAGACAATTACACTTTTGTCGTTCACACCACAAACCCGGTGCCTTATGCGGTGGGTTTGACGACCCACCAATCCTTGTTACCGTTGCCGCAAAAAATCGTGGAAAAATTCGGTGACGATTGGGTGaaaaa                                                                                                                                                                                                     >  1:786615/1‑224 (MQ=255)                                                               ccTTACGCCAGTTTCCTGGATTATCTTCAAGTTGAAAACGCACAAGACATTATTGACGGCAAGAAAAAACCGGAAGAACTTGGCGTGGAAGCAAAAGACAATTACACTTTTGTCGTTCACACCACAAACCCGGTGCCTTATGCGGTGGGTTTGACGACCCACCAATCCTTGTTACCGTTGCCGCAAAAAATCGTGGAAAAATTCGGTGACGATTGGGTGAAAAAAGACAATATCGTCGGTAACGGTGCCta                                                                                                                                                                          >  1:671749/1‑251 (MQ=255)                                                               ccTTACGCCAGTTTCCTGGATTATCTTCAAGTTGAAAACGCACAAGACATTATTGACGGCAAGAAAAAACCGGAAGAACTTGGCGTGGAAGCAAAAGACAATTACACTTTTGTCGTTCACACCACAAACCCGGTGCCTTATGCGGTGGGTTTGACGACCCACCAATCCTTGTTACCGTTGCCGCAAAAAATCGTGGAAAAATTCGGTGACGATTGGGTGAAAAAAGACAATATCGTCGGTAACGGTGCCta                                                                                                                                                                          >  1:377673/1‑251 (MQ=255)                                                                 ttACGCCAGTTTCCTGGATTATCTTCAAGTTGAAAACGCACAAGACATTATTGACGGCAAGAAAAAACCGGAAGAACTTGGCGTGGAAGCAAAAGACAATTACACTTTTGTCGTTCACACCACAAACCCGGTGCCTTATGCGGTGGGTTTGACGACCCACCAATCCTTGTTACCGTTGCCGCAAAAAATCGTGGAAAAATTCGGTGACGATTGGGTGAAAAAAGACAATATCGTCGGTAACGGTGCCtata                                                                                                                                                                        <  1:34616/251‑1 (MQ=255)                                                                      ccAGTTTCCTGGATTATCTTCAAGTTGAAAACGCACAAGACATTATTGACGGCAAGAAAAAACCGGAAGAACTTGGCGTGGAAGCAAAAGACAATTACACTTTTGTCGTTCACACCACAAACCCGGTGCCTTATGCGGTGGGTTTGACGACCCACCAATCCTTGTTACCGTTGCCGCAAAAAATCGTGGAAAAATTCGGTGACGATTGGGTGAAAAAAGACAATATCGTCGGTAACGGTGCCTATAAAtt                                                                                                                                                                    <  1:81525/250‑1 (MQ=255)                                                                           ttCCTGGATTATCTTCAAGTTGAAAACGCACAAGACATTATTGACGGCAAGAAAAAACCGGAAGAACTTGGCGTGGAAGCAAAAGACAATTACACTTTTGTCGTTCACACCACAAACCCGGTGCCTTATGCGGTGGG‑TTGACGACCCACCAATCCTTGTTACCGTTGCCGCAAAAAATCGTGGAAAAATTCGGTGACGATTGGGTGAAAAAAGACAATATCGTCGGTAACGGTGCCtat                                                                                                                                                                         >  1:503023/1‑239 (MQ=255)                                                                                ggATTATCTTCAAGTTGAAAACGCACAAGACATTATTGACGGCAAGAAAAAACCGGAAGAACTTGGCGTGGAAGCAAAAGACAATTACACTTTTGTCGTTCACACCACAAACCCGGTGCCTTATGCGGTGGGTTTGACGACCCACCAATCCTTGTTACCGTTGCCGCAAAAAATCGTGGAAAAATTCGGTGACGATTGGGTGAAAAAAGACAATATCGTCGGTAACGGTGCCTATAAATTAAAAAATCACg                                                                                                                                                         >  1:792428/1‑251 (MQ=255)                                                                                            aGTTGAAAACGCACAAGACATTATTGACGGCAAGAAAAAACCGGAAGAACTTGGCGTGGAAGCAAAAGACAATTACACTTTTGTCGTTCACACCACAAACCCGGTGCCTTATGCGGTGGGTTTGACGACCCACCAATCCTTGTTACCGTTGCCGCAAAAAATCGTGGAAAAATTCGGTGACGATTGGGTGAAAAAAGACAATATCGTCGGTAACGGTGCCTATAAATTAAAAAATCACGTCATCAACGaaa                                                                                                                                             >  1:117033/1‑251 (MQ=255)                                                                                                      gCACAAGACATTATTGACGGCAAGAAAAAACCGGAAGAACTTGGCGTGGAAGCAAAAGACAATTACACTTTTGTCGTTCACACCACAAACCCGGTGCCTTATGCGGTGGGTTTGACGACCCACCAATCCTTGTTACCGTTGCCGCAAAAAATCGTGGAAAAATTCGGTGACGATTGGGTGAAAAAAGACAATATCGTCGGTAACGGTGCCTATAAATTAAAAAATCACGtcatc                                                                                                                                                    <  1:49994/234‑1 (MQ=255)                                                                                                           aGACATTATTGACGGCAAGAAAAAACCGGAAGAACTTGGCGTGGAAGCAAAAGACAATTACACTTTTGTCGTTCACACCACAAACCCGGTGCCTTATGCGGTGGGTTTGACGACCCACCAATCCTTGTTACCGTTGCCGCAAAAAATCGTGGAAAAATTCGGTGACGATTGGGTGAAAAAAGACAATATCGTCGGTAACGGTGCCTATAAATTAAAAAATCACGTCATCAACGAAAAAATTGAATTTGAg                                                                                                                               >  1:209006/1‑250 (MQ=255)                                                                                                             aCATTATTGACGGCAAGAAAAAACCGGAAGAACTTGGCGTGGAAGCAAAAGACAATTACACTTTTGTCGTTCACACCACAAACCCGGTGCCTTATGCGGTGGGTTTGACGACCCACCAATCCTTGTTACCGTTGCCGCAAAAAATCGTGGAAAAATTCGGTGACGATTGGGTGAAAAAAGACAATATCGTCGGTAACGGTGCCTATAAATTAAAAAATCACGTCATCAACGAAAAAATTGAATTTGAgcg                                                                                                                             >  1:726610/1‑250 (MQ=255)                                                                                                             aCATTATTGACGGCAAGAAAAAACCGGAAGAACTTGGCGTGGAAGCAAAAGACAATTACACTTTTGTCGTTCACACCACAAACCCGGTGCCTTATGCGGTGGGTTTGACGACCCACCAATCCTTGTTACCGTTGCCGCAAAAAATCGTGGAAAAATTCGGTGACGATTGGGTGAAAAAAGACAATATCGTCGGTAACGGTGCCTATAAATTAAAAAATCACGTCATCAACGAAAAAATTGAATTTGAgcg                                                                                                                             >  1:317170/1‑250 (MQ=255)                                                                                                             aCATTATTGACGGCAAGAAAAAACCGGAAGAACTTGGCGTGGAAGCAAAAGACAATTACACTTTTGTCGTTCACACCACAAACCCGGTGCCTTATGCGGTGGGTTTGACGACCCACCAATCCTTGTTACCGTTGCCGCAAAAAATCGTGGAAAAATTCGGTGACGATTGGGTGAAAAAAGACAATATCGTCGGTAACGGTGCCTATAAATTAAAAAATCACGTCATCAACGAAAAAATTGAATTTGAgcg                                                                                                                             >  1:133035/1‑250 (MQ=255)                                                                                                             aCATTATTGACGGCAAGAAAAAACCGGAAGAACTTGGCGTGGAAGCAAAAGACAATTACACTTTTGTCGTTCACACCACAAACCCGGTGCCTTATGCGGTGGGTTTGACGACCCACCAATCCTTGTTACCGTTGCCGCAAAAAATCGTGGAAAAATTCGGTGACGATTGGGTGAAAAAAGACAATATCGTCGGTAACGGTGCCTATAAATTAAAAAATCACGTCATCAACGAAAAAATTGAATTTGAgcg                                                                                                                             >  1:327680/1‑250 (MQ=255)                                                                                                             aCATTATTGACGGCAAGAAAAAACCGGAAGAACTTGGCGTGGAAGCAAAAGACAATTACACTTTTGTCGTTCACACCACAAACCCGGTGCCTTATGCGGAGGGTTTGACGACCCACCAATCCTTGTTATCGTTGCCGCAAAAAATCGTGGAAAAATTCGGTGACGATTGGGTGAAAAAAGACAATATCGTCGGTAACGGTGCCTATAAATTAAAAAATCACGTCATCAACGAAAAAATTGAATTTGAgcg                                                                                                                             >  1:688255/1‑250 (MQ=255)                                                                                                                 tattGACGGCAAGAAAAAACCGGAAGAACTTGGCGTGGAAGCAAAAGACAATTACACTTTTGTCGTTCACACCACAAACCCGGTGCCTTATGCGGTGGGTTTGACGACCCACCAATCCTTGTTACCGTTGCCGCAAAAAATCGTGGAAAAATTCGGTGACGATTGGGTGAAAAAAGACAATATCGTCGGTAACGGTGCCTATAAATTAAAAAATCACGTCATCAACGAAAAAATTGAATTTGAGCGCAAcc                                                                                                                        <  1:333095/251‑1 (MQ=255)                                                                                                                  attGACGGCAAGAAAAAACCGGAAGAACTTGGCGTGGAAGCAAAAGACAATTACACTTTTGTCGTTCACACCACAAACCCGGTGCCTTATGCGGTGGGTTTGACGACCCACCAATCCTTGTTACCGTTGCCGCAAAAAATCGTGGAAAAATTCGGTGACGATTGGGTGAAAAAAGACAATATCGTCGGTAACGGTGCCTATAAATTAAAAAATCACGTCATCAACGAAAAAATTGAATTTGAGCGCAAcc                                                                                                                        >  1:742166/1‑250 (MQ=255)                                                                                                                       cGGCAAGAAAAAACCGGAAGAACTTGGCGTGGAAGCAAAAGACAATTACACTTTTGTCGTTCACACCACAAACCCGGTGCCTTATGCGGTGGGTTTGACGACCCACCAATCCTTGTTACCGTTGCCGCAAAAAATCGTGGAAAAATTCGGTGACGATTGGGTGAAAAAAGACAATATCGTCGGTAACGGTGCCTATAAATTAAAAAATCACGTCATCAACGAAAAAATTGAATTTGAGCGCAACCCGCAat                                                                                                                  <  1:769767/251‑1 (MQ=255)                                                                                                                          cAAGAAAAAACCGGAAGAACTTGGCGTGGAAGCAAAAGACAATTACACTTTTGTCGTTCACACCACAAACCCGGTGCCTTATGCGGTGGGTTTGACGACCCACCAATCCTTGTTACCGTTGCCGCAAAAAATCGTGGAAAAATTCGGTGACGATTGGGTGAAAAAAGACAATATCGTCGGTAACGGTGCCTATAAATTAAAAAATCACGTCATCAACGAAAAAATTGAATTTGAGCGCAACCCGCAatat                                                                                                                <  1:689525/250‑1 (MQ=255)                                                                                                                              aaaaaaCCGGAAGAACTTGGCGTGGAAGCAAAAGACAATTACACTTTTGTCGTTCACACCACAAACCCGGTGCCTTATGCGGTGGGTTTGACGACCCACCAATCCTTGTTACCGTTGCCGCAAAAAATCGTGGAAAAATTCGGTGACGATTGGGTGAAAAAAGACAATATCGTCGGTAACGGTGCCTATAAATTAAAAAATCACGTCATCAACGAGAAAATTGAATTTGAGCGCAACCCGCAATATTGGaa                                                                                                           <  1:524590/251‑1 (MQ=255)                                                                                                                              aaaaaaCCGGAAGAACTTGGCGTGGAAGCAAAAGACAATTACACTTTTGTCGTTCACACCACAAACCCGGTGCCTTATGCGGTGGGTTTGACGACCCACCAATCCTTGTTACCGTTGCCGCAAAAAATCGTGGAAAAATTCGGTGACGATTGGGTGAAAAAAGACAATATCGTCGGTAACGGTGCCTATAAATTAAAAAATCACGTCATCAACGAAAAAATTGAATTTGAGCGCAACCCGCAATATTGGaa                                                                                                           <  1:285375/251‑1 (MQ=255)                                                                                                                                       gaagaaCTTGGCGTGGAAGCAAAAGACAATTACACTTTTGTCGTTCACACCACAAACCCGGTGCCTTATGCGGTGGGTTTGACGACCCACCAATCCTTGTTACCGTTGCCGCAAAAAATCGTGGAAAAATTCGGTGACGATTGGGTGAAAAAAGACAATATCGTCGGTAACGGTGCCTATAAATTAAAAAATCACGTCATCAACGAAAAAATTGAATTTGAGCGCAACCCGCAATATTGGAATGACAAAGa                                                                                                  >  1:73795/1‑251 (MQ=255)                                                                                                                                                         gCAAAAGACAATTACACTTTTGTCGTTCACACCACAAACCCGGTGCCTTATGCGGTGGGTTTGACGACCCACCAATCCTTGTTACCGTTGCCGCAAAAAATCGTGGAAAAATTCGGTGACGATTGGGTGAAAAAAGACAATATCGTCGGTAACGGTGCCtata                                                                                                                                                                        >  1:53943/1‑163 (MQ=255)                                                                                                                                                            aaaGACAATTACACTTTTGTCGTTCACACCACAAACCCGGTGCCTTATGCGGTGGGTTTGACGACCCACCAATCCTTGTTACCGTTGCCGCAAAAAATCGTGGAAAAATTCGGTGACGATTGGGTGAAAAAAGACAatat                                                                                                                                                                                            <  1:579171/140‑1 (MQ=255)                                                                                                                                                                 cAATTACACTTTTGTCGTTCACACCACAAACCCGGTGCCTTATGCGGTGGGTTTGACGACCCACCAATCCTTGTTACCGTTGCCGCAAAAAATCGTGGAAAAATTCGGTGACGATTGGGTGAAAAAAGACAATATCGTCGGTAACGGTGCCTATAAATTAAAAAATCACGTCATCAACGAAAAAATTGAATTTGAGCGCAACCCGCAATATTGGAATGACAAAGAAACGCAAATTAACAACGCCACctt                                                                          >  1:821066/1‑249 (MQ=255)                                                                                                                                                                     tACACTTTTGTCGTTCACACCACAAACCCGGTGCCTTATGCGGTGGGTTTGACGACCCACCAATCCTTGTTACCGTTGCCGCAAAAAATCGTGGAAAAATTCGGTGACGATTGGGTGAAAAAAGACAATATCGTCGGTAACGGTGCCTATAAATTAAAAAATCACGTCATCAACGAAAAAATTGAATTTGAGCGCAACCCGCAATATTGGAATGACAAAGAAACGCAAATTAACAACGCCACCTTCTTAGc                                                                    <  1:218122/251‑1 (MQ=255)                                                                                                                                                                       cacTTTTGTCGTTCACACCACAAACCCGGTGCCTTATGCGGTGGGTTTGACGACCCACCAATCCTTGTTACCGTTGCCGCAAAAAATCGTGGAAAAATTCGGTGACGATTGGGTGAAAAAAGACAATATCGTCGGTAACGGTGCCTATAAATTAAAAAATCACGTCATCAACGAAAAAATTGAATTTGAGCGCAACCCGCAATATTGGaa                                                                                                           <  1:176135/210‑1 (MQ=255)                                                                                                                                                                       cacTTTTGTCGTTCACACCACAAACCCGGTGCCTTATGCGGTGGGTTTGACGACCCACCAATCCTTGTTACCGTTGCCGCAAAAAATCGTGGAAAAATTCGGTGACGATTGGGTGAAAAAAGACAATATCGTCGGTAACGGTGCCTATAAATTAAAAAATCACGTCATCAACGAAAAAATTGAATTTGAGCGCAACCCGCAATATTGGAATGACAAAGAAACGCAAATTAACAACGCCACCTTCTTAGCCa                                                                  >  1:13787/1‑251 (MQ=255)                                                                                                                                                                        acTTTTGTCGTTCACACCACAAACCCGGTGCCTTATGCGGTGGGTTTGACGACCCACCAATCCTTGTTACCGTTGCCGCAAAAAATCGTGGAAAAATTCGGTGACGATTGGGTGAAAAAAGACAATATCGTCGGTAACGGTGCCTATAAATTAAAAAATCACGTCATCAACGAAAAAATTGAATTTGAGCGCAACCCGCAATATTGGAATGACAAAGAAACGCAAATTAACAACGCCACCTTCTTAGCCAt                                                                 >  1:504405/1‑251 (MQ=255)                                                                                                                                                                         cTTTTGTCGTTCACACCACAAACCCGGTGCCTTATGCGGTGGGTTTGACGACCCACCAATCCTTGTTACCGTTGCCGCAAAAAATCGTGGAAAAATTCGGTGACGATTGGGTGAAAAAAGACAATATCGTCGGTAACGGTGCCTATAAATTAAAAAATCACGTCATCAACGAAAAAATTGAATTTGAGCGCAACCCGCAATATTg                                                                                                              <  1:7378/205‑1 (MQ=255)                                                                                                                                                                         cTTTTGTCGTTCACACCACAAACCCGGTGCCTTATGCGGTGGGTTTGACGACCCACCAATCCTTGTTACCGTTGCCGCAAAAAATCGTGGAAAAATTCGGTGACGATTGGGTGAAAAAAGACAATATCGTCGGTAACGGTGCCTATAAATTAAAAAATCACGTCATCAACGAAAAAATTGAATTTGAGCGCAACCCGCAATATTGGAATGACAAAGAAACGCAAATTAACAACGCCACCTTCTTAGc                                                                    >  1:586630/1‑247 (MQ=255)                                                                                                                                                                         cTTTTGTCGTTCACACCACAAACCCGGTGCCTTATGCGGTGGGTTTGACGACCCACCAATCCTTGTTACCGTTGCCGCAAAAAATCGTGGAAAAATTCGGTGACGATTGGGTGAAAAAAGACAATATCGTCGGTAACGGTGCCTATAAATTAAAAAATCACGTCATCAACGAAAAAATTGAATTTGAGCGCAACCCGCAATATTGGAATGACAAAGAAACGCAAATTAACAACGCCACCTTCTTAGCCAtt                                                                >  1:65508/1‑251 (MQ=255)                                                                                                                                                                                   tCACACCACAAACCCGGTGCCTTATGCGGTGGGTTTGACGACCCACCAATCCTTGTTACCGTTGCCGCAAAAAATCGTGGAAAAATTCGGTGACGATTGGGTGAAAAAAGACAATATCGTCGGTAACGGTGCCTATAAATTAAAAAAt                                                                                                                                                             >  1:814553/1‑148 (MQ=255)                                                                                                                                                                                       accacAAACCCGGTGCCTTATGCGGTGGGTTTGACGACCCACCAATCCTTGTTACCGTTGCCGCAAAAAATCGTGGAAAAATTCGGTGACGATTGGGTGAAAAAAGACAATATCGTCGGTAACGGTGCCTATAAATTAAAAAATCACGTCATCAACGAAAAAATTGAATTTGAGCGCAACCCGCAATATTGGAATGACAAAGAAACGCAAATTAACAACGCCACCTTCTTAGCCATTGAGAATTCCAGCAc                                                  <  1:274922/251‑1 (MQ=255)                                                                                                                                                                                        ccacAAACCCGGTGCCTTATGCGGTGGGTTTGACGACCCACCAATCCTTGTTACCGTTGCCGCAAAAAATCGTGGAAAAATTCGGTGACGATTGGGTGAAAAAAGACAATATCGTCGGt                                                                                                                                                                                     <  1:54721/119‑1 (MQ=255)                                                                                                                                                                                        ccacAAACCCGGTGCCTTATGCGGTGGGTTTGACGACCCACCAATCCTTGTTACCGTTGCCGCAAAAAATCGTGGAAAAATTCGGTGACGATTGGGTGAAAAAAGACAATATCGTCGGTAACGGTGCCTATAAATTAAAAAATCACGTCATCAACGAAAAAATTGAATTTGAGCGCAACCCGCAATATTGGAATGACAAAGAAACGCAAATTAACAACGCCACCTTCTTAGCCATTGAGAATTCCAGCAc                                                  >  1:851321/1‑250 (MQ=255)                                                                                                                                                                                        ccacAAACCCGGTGCCTTATGCGGTGGGTTTGACGACCCACCAATCCTTGTTACCGTTGCCGCAAAAAATCGTGGAAAAATTCGGTGACGATTGGGTGAAAAAAGACAATATCGTCGGTAACGGTGCCTATAAATTAAAAAATCACGTCATCAACGAAAAAATTGAATTTGAGCGCAACCCGCAATATTGGAATGACAAAGAAACGCAAATTAACAACGCCACCTTCTTAGCCATTGAGAATTCCAGCAc                                                  >  1:816087/1‑250 (MQ=255)                                                                                                                                                                                        ccacAAACCCGGTGCCTTATGCGGTGGGTTTGACGACCCACCAATCCTTGTTACCGTTGCCGCAAAAAATCGTGGAAAAATTCGGTGACGATTGGGTGAAAAAAGACAATATCGTCGGTAACGGTGCCTATAAATTAAAAAATCACGTCATCAACGAAAAAATTGAATTTGAGCGCAACCCGCAATATTGGAATGACAAAGAAACGCAAATTAACAACGCCACCTTCTTAGCCATTGAGAATTCCAGCAc                                                  >  1:687173/1‑250 (MQ=255)                                                                                                                                                                                        ccacAAACCCGGTGCCTTATGCGGTGGGTTTGACGACCCACCAATCCTTGTTACCGTTGCCGCAAAAAATCGTGGAAAAATTCGGTGACGATTGGGTGAAAAAAGACAATATCGTCGGTAACGGTGCCTATAAATTAAAAAATCACGTCATCAACGAAAAAATTGAATTTGAGCGCAACCCGCAATATTGGAATGACAAAGAAACGCAAATTAACAACGCCACCTTCTTAGCCATTGAGAATTCCAGCAc                                                  <  1:229263/250‑1 (MQ=255)                                                                                                                                                                                                   gTGCCTTATGCGGTGGGTTTGACGACCCACCAATCCTTGTTACCGTTGCCGCAAAAAATCGTGGAAAAATTCGGTGACGATTGGGTGAAAAAAGACAATATCGTCGGTAACGGTGCCTATAAATTAAAAAATCACGTCATCAACGAAAAAATTGAATTTGAGCGCAACCCGCAATATTGGAATGACAAAGAAACGCAAATTaac                                                                                     >  1:659467/1‑204 (MQ=255)                                                                                                                                                                                                   gTGCCTTATGCGGTGGGTTTGACGACCCACCAATCCTTGTTACCGTTGCCGCAAAAAATCGTGGAAAAATTCGGTGACGATTGGGTGAAAAAAGACAATATCGTCGGTAACGGTGCCTATAAATTAAAAAATCACGTCATCAACGAAAAAATTGAATTTGAGCGCAACCCGCAATATTGGAATGACAAAGAAACGCAAATTAACAACGCCACCTTCTTAGCCATTGAGAATTCCAGCACCGACGTGGCACg                                      >  1:18979/1‑251 (MQ=255)                                                                                                                                                                                                    aacaaggattggTGGGTTTGACGACCCACCAATCCTTGTTACCGTTGCCGCAAAAAATCGTGGAAAAATTCGGTGACGATTGGGTGAAAAAAGACAATATCGTCGGTAACGGTGCCTATAAATTAAAAAATCACGTCATCAACGAAAAAATTGAATTTGAGCGCAACCCGCAATATTGGAATGACAAAGAAACGCAAATTAACAACGCCACCTTCTTAGCCATTGAGAATTCCAGCAc                                                  <  1:155637/228‑1 (MQ=255)                                                                                                                                                                                                     gCCTTATGCGGTGGGTTTGACGACCCACCAATCCTTGTTACCGTTGCCGCAAAAAATCGTGGAAAAATTCGGTGACGATTGGGTGAAAAAAGACAATATCGTCGGTAACGGTGCCTATAAATTAAAAAATCACGTCATCAACGAAAAAATTGAATTTGAGCGCAACCCGCAATATTGGAATGACAAAGAAACGCAAATTAACAACGCCACCTTCTTAGCCATTGAGAATTCCAGCACCGACGTGGCACGtt                                    >  1:488600/1‑251 (MQ=255)                                                                                                                                                                                                     gCCTTATGCGGTGGGTTTGACGACCCACCAATCCTTGTTACCGTTGCCGCAAAAAATCGTGGAAAAATTCGGTGACGATTGGGTGAAAAAAGACAATATCGTCGGTAACGGTGCCTATAAATTAAAAAATCACGTCATCAACGAAAAAATTGAATTTGAGCGCAACCCGCAATATTGGAATGACAAAGAAACGCAAATTAACAACGCCACCTTCTTAGCCATTGAGAATTCCAGCACCGACGTGGCACGtt                                    >  1:395477/1‑251 (MQ=255)                                                                                                                                                                                                       cTTATGCGGTGGGTTTGACGACCCACCAATCCTTGTTACCGTTGCCGCAAAAAATCGTGGAAAAATTCGGTGACGATTGGGTGAAAAAAGACAATATCGTCGGTAACGGTGCCTATAAATTAAAAAATCACGTCATCAACGAAAAAATTGAATTTGAGCGCAACCCGCAATATTGGAATGACAAAGAAACGCAAATTAACAACGCCACCTTCTTAGCCATTgag                                                             >  1:58080/1‑224 (MQ=255)                                                                                                                                                                                                       cTTATGCGGTGGGTTTGACGACCCACCAATCCTTGTTACCGTTGCCGCAAAAAATCGTGGAAAAATTCGGTGACGATTGGGTGAAAAAAGACAATATCGTCGGTAACGGTGCCTATAAATTAAAAAATCACGTCATCAACGAAAAAATTGAATTTGAGCGCAACCCGCAATATTGGAATGACAAAGAAACGCAAATTAACAACGCCACCTTCTTAGCCATTGAGAATTCCAGCACCGACGTGGCACGTTAt                                  <  1:311548/251‑1 (MQ=255)                                                                                                                                                                                                       cTTATGCGGTGGGTTTGACGACCCACCAATCCTTGTTACCGTTGCCGCAAAAAATCGTGGAAAAATTCGGTGACGATTGGGTGAAAAAAGACAATATCGTCGGTAACGGTGCCTATAAATTAAAAAATCACGTCATCAACGAAAAAATTGAATTTGAGCGCAACCCGCAATATTGGAATGACAAAGAAACGCAAATTAACAACGCCACCTTCTTAGCCATTGAGAATTCCAGCACCGACGTGGCACGTTAt                                  <  1:732292/251‑1 (MQ=255)                                                                                                                                                                                                             cGGTGGGTTTGACGACCCACCAATCCTTGTTACCGTTGCCGCAAAAAATCGTGGAAAAATTCGGTGACGATTGGGTGAAAAAAGACAATATCGTCGGTAACGGTGCCTATAAATTAAAAAATCACGTCATCAACGAAAAAATTGAATTTGAGCGCAACCCGCAATATTGGAATGACAAAGAAACGCAAATTAACAACGCCACCTTCTTAGCCATTGAGAATTCCAGCACCGACGTGGCACGTTATCGTGc                             <  1:606913/250‑1 (MQ=255)                                                                                                                                                                                                             cGGTGGGTTTGACGACCCACCAATCCTTGTTACCGTTGCCGCAAAAAATCGTGGAAAAATTCGGTGACGATTGGGTGAAAAAAGACAATATCGTCGGTAACGGTGCCTATAAATTAAAAAATCACGTCATCAACGAAAAAATTGAATTTGAGCGCAACCCGCAATATTGGAATGACAAAGAAACGCAAATTAACAACGCCACCTTCTTAGCCATTGAGAATTCCAGCACCGACGTGGCACGTTATCGTGc                             <  1:325826/250‑1 (MQ=255)                                                                                                                                                                                                               gTGGGTTTGACGACCCACCAATCCTTGTTACCGTTGCCGCAAAAAATCGTGGAAAAATTCGGTGACGATTGGGTGAAAAAAGACAATATCGTCGGTAACGGTGCCTATAAATTAAAAAATCACGTCATCAACGAAAAAATTGAATTTGAGCGCAACCCGCAATATTGGAATGACAAAGAAACGCAAATTAACAACGCCACCTTCTTAGCCATTGAGAATTCCAGCACCGACGTGGCACGTTATCGTGCAg                           <  1:144098/250‑1 (MQ=255)                                                                                                                                                                                                                  ggTTTGACGACCCACCAATCCTTGTTACCGTTGCCGCAAAAAATCGTGGAAAAATTCGGTGACGATTGGGTGAAAAAAGACAATATCGTCGGTAACGGTGCCTATAAATTAAAAAATCACGTCATCAACGAAAAAATTGAATTTGAGCGCAACCCGCAATATTGGAATGACAAAGAAACGCAAATTAACAACGCCACCTTCTTAGCCATTGAGAATTCCAGCACCGACGTGGCACGTTATCGTGCAGgaga                       >  1:203200/1‑251 (MQ=255)                                                                                                                                                                                                                    tttGACGACCCACCAATCCTTGTTACCGTTGCCGCAAAAAATCGTGGAAAAATTCGGTGACGATTGGGTGAAAAAAGACAATATCGTCGGTAACGGTGCCTATAAATTAAAAAATCACGTCATCAACGAAAAAATTGAATTTGAGCGCAACCCGCAATATTGGAATGACAAAGAAACGCAAATTAACAACGCCACCTTCTTAGCCATTGAGAATTCCAGCACCGACGTGGCACGTTATCGTGCAGGAGAtt                     >  1:734852/1‑251 (MQ=255)                                                                                                                                                                                                                         cgacCCACCAATCCTTGTTACCGTTGCCGCAAAAAATCGTGGAAAAATTCGGTGACGATTGGGTGAAAAAAGACAATATCGTCGGTAACGGTGCCTATAAATTAAAAAATCACGTCATCAACGAAAAAATTGAATTTGAGCGCAACCCGCAATATTGGAATGACAAAGAAACGCAAATTAACAACGCCACCTTCTTAGCCATTGAGAATTCCAGCACCGACGTGGCACGTTATCGTGCAGGAGATTTGGAc                <  1:254149/251‑1 (MQ=255)                                                                                                                                                                                                                         cgacCCACCAATCCTTGTTACCGTTGCCGCAAAAAATCGTGGAAAAATTCGGTGACGATTGGGTGAAAAAAGACAATATCGTCGGTAACGGTGCCTATAAATTAAAAAATCACGTCATCAACGAAAAAATTGAATTTGAGCGCAACCCGCAATATTGGAATGACAAAGAAACGCAAATTAACAACGCCACCTTCTTAGCCATTGAGAATTCCAGCACCGACGTGGCACGTTATCGTGCAGGAGATTTGGAc                <  1:496117/251‑1 (MQ=255)                                                                                                                                                                                                                             ccaccaATCCTTGTTACCGTTGCCGCAAAAAATCGTGGAAAAATTCGGTGACGATTGGGTGAAAAAAGACAATATCGTCGGTAACGGTGCCTATAAATTAAAAAATCACGTCATCAACGAAAAAATTGAATTTGAGCGCAACCCGCAATATTGGAATGACAAAGAAACGCAAATTAACAACGCCACCTTCTTAGCCATTGAGAATTCCAGCACCGACGTGGCACGTTATCGTGCAGgag                        <  1:124460/239‑1 (MQ=255)                                                                                                                                                                                                                                   aTCCTTGTTACCGTTGCCGCAAAAAATCGTGGAAAAATTCGGTGACGATTGGGTGAAAAAAGACAATATCGTCGGTAACGGTGCCTATAAATTAAAAAATCACGTCATCAACGAAAAAATTGAATTTGAGCGCAACCCGCAATATTGGAATGACAAAGAAACGCAAATTAACAACGCCACCTTCTTAGCCATTGAGAATTCCAGCACCGACGTGGCACGTTATCGTGCAGGAGATTTGGACATCACCAGcc      <  1:523578/251‑1 (MQ=255)                                                                                                                                                                                                                                   aTCCTTGTTACCGTTGCCGCAAAAAATCGTGGAAAAATTCGGTGACGATTGGGTGAAAAAAGACAATATCGTCGGTAACGGTGCCTATAAATTAAAAAATCACGTCATCAACGAAAAAATTGAATTTGAGCGCAACCCGCAATATTGGAATGACAAAGAAACGCAAATTAACAACGCCACCTTCTTAGCCATTGAGAATTCCAGCACCGACGTGGCACGTTATCGTGCAGGAGATTTGGACATCACCAGcc      <  1:13850/251‑1 (MQ=255)                                                                                                                                                                                                                                    tCCTTGTTACCGTTGCCGCAAAAAATCGTGGAAAAATTCGGTGACGATTGGGTGAAAAAAGACAATATCGTCGGTAACGGTGCCTATAAATTAAAAAATCACGTCATCAACGAAAAAATTGAAtt                                                                                                                                   <  1:415883/125‑1 (MQ=255)                                                                                                                                                                                                                                    tCCTTGTTACCGTTGCCGCAAAAAATCGTGGAAAAATTCGGTGACGATTGGGTGAAAAAAGACAATATCGTCGGTAACGGTGCCTATAAATTAAAAAATCACGTCATCAACGAAAAAATTGAATTTGAGCGCAACCCGCAATATTGGAATGACAAAGAAACGCAAATTAACAACGCCACCTTCTTAGCCATTGAGAATTCCAGCACCGACGTGGCACGTTATCGTGCAGGAGATTTGGACATCACCAGCCa     <  1:739193/251‑1 (MQ=255)                                                                                                                                                                                                                                     ccTTGTTACCGTTGCCGCAAAAAATCGTGGAAAAATTCGGTGACGATTGGGTGAAAAAAGACAATATCGTCGGTATCGGTGCCTATAAATTAAAAAATCACGTCATCAACGAAAAAATTGAATTTGAGCGCAACCCGCAATATTGGAATGACAAAGAAACGCAAATTAACAACGCCACCTTCTTAGCCATTGAGAATTCCAGCACCGTCGTGGCACGTTATCg                                <  1:503804/223‑1 (MQ=255)                                                                                                                                                                                                                                     ccTTGTTACCGTTGCCGCAAAAAATCGTGGAAAAATTCGGTGACGATTGGGTGAAAAAAGACAATATCGTCGGTAACGGTGCCTATAAATTAAAAAATCACGTCATCAACGAAAAAATTGAATTTGAGCGCAACCCGCAATATTGGAATGACAAAGAAACGCAAATTAACAACGCCACCTTCTTAGCCATTGAGAATTCCAGCAc                                                  >  1:149888/1‑205 (MQ=255)                                                                                                                                                                                                                                     ccTTGTTACCGTTGCCGCAAAAAATCGTGGAAAAATTCGGTGACGATTGGGTGAAAAAAGACAATATCGTCGGTAACGGTGCCTATAAATTAAAAAATCACGTCATCAACGAAAAAATTGAATTTGAGCGCAACCCGCAATATTGGAATGACAAAGAAACGCAAATTAACAACGCCACCTTCTTAGCCATTGAGAATTCCAGCACCGACGTGGCACGTTATCGTGCAGGAGATTTGGACATCACCAGCCa     >  1:410802/1‑250 (MQ=255)                                                                                                                                                                                                                                     ccTTGTTACCGTTGCCGCAAAAAATCGTGGAAAAATTCGGTGACGATTGGGTGAAAAAAGACAATATCGTCGGTAACGGTGCCTATAAATTAAAAAATCACGTCATCAACGAAAAAATTGAATTTGAGCGCAACCCGCAATATTGGAATGACAAAGAAACGCAAATTAACAACGCCACCTTCTTAGCCATTGAGAATTCCAGCACCGACGTGGCACGTTATCGTGCAGGAGATTTGGACATCACCAGCCa     >  1:134498/1‑250 (MQ=255)                                                                                                                                                                                                                                       ttGTTACCGTTGCCGCAAAAAATCGTGGAAAAATTCGGTGACGATTGGGTGAAAAAAGACAATATCGTCGGTAACGGTGCCTATAAATTAAAAAATCACGTCATCAACGAAAAAATTGAATTTGAGCGCAACCCGCAATATTGGAATGACAAAGAAACGCAAATTAACAACGCCACCTTCTTAGCCATTGAGAATTCCAGCACCGACg                                             >  1:691173/1‑208 (MQ=255)                                                                                                                                                                                                                                       ttGTTACCGTTGCCGCAAAAAATCGTGGAAAAATTCGGTGACGATTGGGTGAAAAAAGACAATATCGTCGGTAACGGTGCCTATAAATTAAAAAATCACGTCATCAACGAAAAAATTGAATTTGAGCGCAACCCGCAATATTGGAATGACAAAGAAACGCAAATTAACAACGCCACCTTCTTAGCCATTGAGAATTCCAGCACCGACGTGGCACGTTATCGTGCAGGAGATTTGGAc                <  1:153946/237‑1 (MQ=255)                                                                                                                                                                                                                                       ttGTTACCGTTGCCGCAAAAAATCGTGGAAAAATTCGGTGACGATTGGGTGAAAAAAGACAATATCGTCGGTAACGGTGCCTATAAATTAAAAAATCACGTCATCAACGAAAAAATTGAATTTGAGCGCAACCCGCAATATTGGAATGACAAAGAAACGCAAATTAACAACGCCACCTTCTTAGCCATTGAGAATTCCAGCACCGACGTGGCACGTTATCGTGCAGGAGATTTGGACATCACCAGcc      >  1:39992/1‑247 (MQ=255)                                                                                                                                                                                                                                       ttGTTACCGTTGCCGCAAAAAATCGTGGAAAAATTCGGTGACGATTGGGTGAAAAAAGACAATATCGTCGGTAACGGTGCCTATAAATTAAAAAATCACGTCATCAACGAAAAAATTGAATTTGAGCGCAACCCGCAATATTGGAATGACAAAGAAACGCAAATTAACAACGCCACCTTCTTAGCCATTGAGAATTCCAGCACCGACGTGGCACGTTATCGTGCAGGAGATTTGGACATCACCAGCCACGc  <  1:535354/251‑1 (MQ=255)                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                CTATTACGGCAAATGATTTCGTCTTCGCCTGGCGTCGTTTAGTAACACCGGCAACCGCCTCACCTTACGCCAGTTTCCTGGATTATCTTCAAGTTGAAAACGCACAAGACATTATTGACGGCAAGAAAAAACCGGAAGAACTTGGCGTGGAAGCAAAAGACAATTACACTTTTGTCGTTCACACCACAAACCCGGTGCCTTATGCGGTGGGTTTGACGACCCACCAATCCTTGTTACCGTTACCGCAAAAAATCGTGGAAAAATTCGGTGACGATTGGGTGAAAAAAGACAATATCGTCGGTAACGGTGCCTATAAATTAAAAAATCACGTCATCAACGAAAAAATTGAATTTGAGCGCAACCCGCAATATTGGAATGACAAAGAAACGCAAATTAACAACGCCACCTTCTTAGCCATTGAGAATTCCAGCACCGACGTGGCACGTTATCGTGCAGGAGATTTGGACATCACCAGCCACGC  >  1/2031368‑2031848

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑