Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	2,027,397	G→A	G137S (GGC→AGC)	unknown →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	2,027,397	0	G	A	100.0%	228.5 / NA	79	G137S (GGC→AGC)	unknown	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (44/35);  total (44/35)

GGATTACGTAAAACGCTGCCAAAACCCACCGCACTTCGTCAGCGTTATCTCCTTTTGATTATGCTGGGCATTCTTGGGCTATCGGCAAATTTCTTCTTATTTAATCTGGCGCTGAACTATATTCCACCGACCAGCAGCCAAGTGCTCAGTCCGTTATCCTCGTTTATCATGCTATTTGTCGGTGTGTTTGTGTTTAAAGAACACATCGGGCTCCATCAAAAAATCGGTTTGGTTATTTTAATTCTCGGCTTGGTCTTATTTTTCAACCAACGCTTTAGTGATTTCTTACAGCTCAATGATT‑ATTTTAAAGGCGTTGTTATTGCCCTATGCGCCAGCTCCTGCTGGGTTGCCTATGGCATGGCGCAAAAGCTGATGTTGAGCCGTTTCAGTTCGCAACAAATTTTGCTCATAATTTATGTCGGCTGCACGATCATTTTTACGCCCTTTGCCAATATTCAGCAGGTGAGCGCGTTAAATTCCTTTCAG  >  1/2027151‑2027636                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                 ggATTACGTAAAACGCTGCCAAAACCCACCGCACTTCGTCAGCGTTATCTCCTTTTGATTATGCTGGGCATTCTTGGGCTATCGGCAAATTTCTTCTTATTTAATCTGGCGCTGAACTATATTCCACCGACCAGCAGCCAAGTGCTCAGTCCGTTATCCTCGTTTATCATGCTATTTGTCGGTGTGTTTGTGTTTAAAGAACACATCGGGCTCCATCAAAAAATCGGTTTGGTTATTTTAATTCTCAGCtt                                                                                                                                                                                                                                              >  1:724643/1‑251 (MQ=255)      aCGTAAAACGCTGCCAAAACCCACCGCACTTCGTCAGCGTTATCTCCTTTTGATTATGCTGGGCATTCTTGGGCTATCGGCAAATTTCTTCTTATTTAATCTGGCGCTGAACTATATTCCACCGACCAGCAGCCAAGTGCTCAGTCCGTTATCCTCGTTTATCATGCTATTTGTCGGTGTGTTTGTGTTTAAAGAACACATCGGGCTCCATCAAAAAATCGGTTTGGTTATTTTAATTCTCAGCTTGGt                                                                                                                                                                                                                                           >  1:366110/1‑249 (MQ=255)      aCGTAAAACGCTGCCAAAACCCACCGCACTTCGTCAGCGTTATCTCCTTTTGATTATGCTGGGCATTCTTGGGCTATCGGCAAATTTCTTCTTATTTAATCTGGCGCTGAACTATATTCCACCGACCAGCAGCCAAGTGCTCAGTCCGTTATCCTCGTTTATCATGCTATTTGTCGGTGTGTTTGTGTTTAAAGAACACATCGGGCTCCATCAAAAAATCGGTTTGGTTATTTTAATTCTCAGCTTGGt                                                                                                                                                                                                                                           >  1:603605/1‑249 (MQ=255)           aaaCGCTGCCAAAACCCACCGCACTTCGTCAGCGTTATCTCCTTTTGATTATGCTGGGCATTCTTGGGCTATCGGCAAATTTCTTCTTATTTAATCTGGCGCTGAACTATATTCCACCGACCAGCAGCCAAGTGCTCAGTCCGTTATCCTCGTTTATCATGCTATTTGTCGGTGTGTTTGTGTTTAAAGAACACATCGGGCTCCATCAAAAAATCGGTTTGGTTATTTTAATTCTCAGCTTGGTCTTAttt                                                                                                                                                                                                                                    <  1:876349/251‑1 (MQ=255)                 tGCCAAAACCCACCGCACTTCGTCAGCGTTATCTCCTTTTGATTATGCTGGGCATTCTTGGGCTATCGGCAAATTTCTTCTTATTTAATCTGGCGCTGAACTATATTCCACCGACCAGCAGCCAAGTGCTCAGTCCGTTATCCTCGTTTATCATGCTATTTGTCGGTGTGTTTGTGTTTAAAGAACACATCGGGCTCCATCAAAAAATCGGTTTGGTTATTTTAATTCTCAGCTTGGTCTTATTTTTcaac                                                                                                                                                                                                                              <  1:141182/251‑1 (MQ=255)                  gCCAAAACCCACCGCACTTCGTCAGCGTTATCTCCTTTTGATTATGCTGGGCATTCTTGGGCTATCGGCAAATTTCTTCTTATTTAATCTGGCGCTGAACTATATTCCACCGACCAGCAGCCAAGTGCTCAGTCCGTTATCCTCGTTTATCATGCTATTTGTCGGTGTGTTTGTGTTTAAAGAACACATCGGGCTCCATCAAAAAATCGGTTTGGTTATTTTAATTCTCAGCTTGGTCTTATTTTTcaac                                                                                                                                                                                                                              >  1:464297/1‑250 (MQ=255)                  gCCAAAACCCACCGCACTTCGTCAGCGTTATCTCCTTTTGATTATGCTGGGCATTCTTGGGCTATCGGCAAATTTCTTCTTATTTAATCTGGCGCTGAACTATATTCCACCGACCAGCAGCCAAGTGCTCAGTCCGTTATCCTCGTTTATCATGCTATTTGTCGGTGTGTTTGTGTTTAAAGAACACATCGGGCTCCATCAAAAAATCGGTTTGGTTATTTTAATTCTCAGCTTGGTCTTATTTTTcaac                                                                                                                                                                                                                              >  1:130662/1‑250 (MQ=255)                                    tCGTCAGCGTTATCTCCTTTTGATTATGCTGGGCATTCTTGGGCTATCGGCAAATTTCTTCTTATTTAATCTGGCGCTGAACTATATTCCACCGACCAGCAGCCAAGTGCTCAGTCCGTTATCCTCGTTTATCATGCTATTTGTCGGTGTGTTTGTGTTTAAAGAACACGTCGGGCTCCATCAAAAAATCGGTTTGGTTATTTTAATTCTCAGCTTGGTCTTATTTTTCAACCAACGCTTTAGTGAttt                                                                                                                                                                                                             >  1:437772/1‑249 (MQ=255)                                    tCGTCAGCGTTATCTCCTTTTGATTATGCTGGGCATTCTTGGGCTATCGGCAAATTTCTTCTTATTTAATCTGGCGCTGAACTATATTCCACCGACCAGCAGCCAAGTGCTCAGTCCGTTATCCTCGTTTATCATGCTATTTGTCGGTGTGTTTGTGTTTAAAGAACACATCGGGCTCCATCAAAAAATCGGTTTGGTTATTTTAATTCTCAGCTTGGTCTTATTTTTCAACCAACGCTTTAGTGAttt                                                                                                                                                                                                             >  1:894801/1‑249 (MQ=255)                                    tCGTCAGCGTTATCTCCTTTTGATTATGCTGGGCATTCTTGGGCTATCGGCAAATTTCTTCTTATTTAATCTGGCGCTGAACTATATTCCACCGACCAGCAGCCAAGTGCTCAGTCCGTTATCCTCGTTTATCATGCTATTTGTCGGTGTGTTTGTGTTTAAAGAACACATCGGGCTCCATCAAAAAATCGGTTTGGTTATTTTAATTCTCAGCTTGGTCTTATTTTTCAACCAACGCTTTAGTGAttt                                                                                                                                                                                                             >  1:420946/1‑249 (MQ=255)                                               aTCTCCTTTTGATTATGCTGGGCATTCTTGGGCTATCGGCAAATTTCTTCTTATTTAATCTGGCGCTGAACTATATTCCACCGACCAGCAGCCAAGTGCTCAGTCCGTTATCCTCGTTTATCATGCTATTTGTCGGTGTGTTTGTGTTTAAAGAACACATCGGGCTCCATCAAAAAATCGGTTTGGTTATTTTAATTCTCAGCTTGGTCTTATTTTTCAACCAACGCTTTAGTGATTTCTTACAGCTCAAt                                                                                                                                                                                                <  1:29473/251‑1 (MQ=255)                                                 ctcCTTTTGATTATGCTGGGCATTCTTGGGCTATCGGCAAATTTCTTCTTATTTAATCTGGCGCTGAACTATATTCCACCGACCAGCAGCCAAGTGCTCAGTCCGTTATCCTCGTTTATCATGCTATTTGTCGGTGTGTTTGTGTTTAAAGAACACATCGGGCTCCATCAAAAAATCGGTTTGGTTATTTTAATTCTCAGCTTGGTCTTATTTTTCAACCAACGCTTTAGTGATTTCTTACAGCTCAATGa                                                                                                                                                                                              <  1:43911/251‑1 (MQ=255)                                                   ccTTTTGATTATGCTGGGCATTCTTGGGCTATCGGCAAATTTCTTCTTATTTAATCTGGCGCTGAACTATATTCCACCGACCAGCAGCCAAGTGCTCAGTCCGTTATCCTCGTTTATCATGCTATTTGTCGGTGTGTTTGTGTTTAAAGAACACATCGGGCTCCATCAAAAAATCGGTTTGGTTATTTTAATTCTCAGCTTGGTCTTATTTTTCAACCAACGCTTTAGTGATTTCTTACAGCTCAATGatt                                                                                                                                                                                            >  1:228150/1‑251 (MQ=255)                                                   ccTTTTGATTATGCTGGGCATTCTTGGGCTATCGGCAAATTTCTTCTTATTTAATCTGGCGCTGAACTATATTCCACCGACCAGCAGCCAAGTGCTCAGTCCGTTATCCTCGTTTATCATGCTATTTGTCGGTGTGTTTGTGTTTAAAGAACACATCGGGCTCCATCAAAAAATCGGTTTGGTTATTTTAATTCTCAGCTTGGTCTTATTTTTCAACCAACGCTTTAGTGATTTCTTACAGCTCAATGatt                                                                                                                                                                                            >  1:722320/1‑251 (MQ=255)                                                        tGATTATGCTGGGCATTCTTGGGCTATCGGCAAATTTCTTCTTATTTAATCTGGCGCTGAACTATATTCCACCGACCAGCAGCCAAGTGCTCAGTCCGTTATCCTCGTTTATCATGCTATTTGTCGGTGTGTTTGTGTTTAAAGAACACATCGGGCTCCATCAAAAAATCGGTTTGGTTATTTTAATTCTCAGCTTGGTCTTATTTTTCAACCAACGCTTTAGTGATTTCTTACAGCTCAATGATT‑Atttt                                                                                                                                                                                      >  1:565147/1‑251 (MQ=255)                                                         gATTATGCTGGGCATTCTTGGGCTATCGGCAAATTTCTTCTTATTTAATCTGGCGCTGAACTATATTCCACCGACCAGCAGCCAAGTGCTCAGTCCGTTATCCTCGTTTATCATGCTATTTGTCGGTGTGTTTGTGTTTAAAGAACACATCGGGCTCCATCAAAAAATCGGTTTGGTTATTTTAATTCTCAGCTTGGTCTTAttttt                                                                                                                                                                                                                                  <  1:476586/207‑1 (MQ=255)                                                                    gCATTCTTGGGCTATCGGCAAATTTCTTCTTATTTAATCTGGCGCTGAACTATATTCCACCGACCAGCAGCCAAGTGCTCAGTCCGTTATCCTCGTTTATCATGCTATTTGTCGGTGTGTTTGTGTTTAAAGAACACATCGGGCTCCATCAAAAAATCGGTTTGGTTATTTTAATTCTCAGCTTGGTCTTATTTTTCAACCAACGCTTTAGTGATTTCTTACAGCTCAATGATT‑ATTTTAAAGGCgttgtt                                                                                                                                                                          >  1:825835/1‑251 (MQ=255)                                                                       ttCTTGGGCTATCGGCAAATTTCTTCTTATTTAATCTGGCGCTGAACTATATTCCACCGACCAGCAGCCAAGTGCTCAGTCCGTTATCCTCGTTTATCATGCTATTTGTCGGTGTGTTTGTGTTTAAAGAACACATCGGGCTCCATCAAAAAATCGGTTTGGTTATTTTAATTCTCAGCTTGGTCTTAttt                                                                                                                                                                                                                                    <  1:187146/191‑1 (MQ=255)                                                                       ttCTTGGGCTATCGGCAAATTTCTTCTTATTTAATCTGGCGCTGAACTATATTCCACCGACCAGCAGCCAAGTGCTCAGTCCGTTATCCTCGTTTATCATGCTATTTGTCGGTGTGTTTGTGTTTAAAGAACACATCGGGCTCCATCAAAAAATCGGTTTGGTTATTTTAATTCTCAGCTTGGTCTTATTTTTCAACCAACGCTTTAGTGATTTCTTACAGCTCAATGATT‑ATTTTAAAgg                                                                                                                                                                                 <  1:797236/241‑1 (MQ=255)                                                                       ttCTTGGGCTATCGGCAAATTTCTTCTTATTTAATCTGGCGCTGAACTATATTCCACCGACCAGCAGCCAAGTGCTCAGTCCGTTATCCTCGTTTATCATGCTATTTGTCGGTGTGTTTGTGTTTAAAGAACACATCGGGCTCCATCAAAAAATCGGTTTGGTTATTTTAATTCTCAGCTTGGTCTTATTTTTCAACCAACGCTTTAGTGATTTCTTACAGCTCAATGATT‑ATTTTAAAGGCGTTGTTAtt                                                                                                                                                                       >  1:594086/1‑251 (MQ=255)                                                                          ttaggCTATCGGCAAATTTCTTCTTATTTAATCTGGCGCTGAACTATATTCCACCGACCAGCAGCCAAGTGCTCAGTCCGTTATCCTCGTTTATCATGCTATTTGTCGGTGTGTTTGTGTTTAAAGAACACATCGGGCTCCATCAAAAAATCGGTTTGGTTATTTTAATTCTCAGCTTGGTCTTATTTTTCAACCAACGCTTTAGTGATTTCTTACAGCTCAATGATT‑ATTTTAAAGGCGTTGTTATTGcc                                                                                                                                                                    <  1:504503/248‑1 (MQ=255)                                                                           tGGGCTATCGGCAAATTTCTTCTTATTTAATCTGGCGCTGAACTATATTCCACCGACCAGCAGCCAAGTGCTCAGTCCGTTATCCTCGTTTATCATGCTATTTGTCGGTGTGTTTGTGTTTAAAGAACACATCGGGCTCCATCAAAAAATCGGTTTGGTTATTTTAATTCTCAGCTTGGTCTTATTTTTCAACCAACGCTTTAGTGATTTCTTACAGCTCAATGATT‑ATTTTAAAGGCGTTGTTATTGccc                                                                                                                                                                   <  1:775596/251‑1 (MQ=255)                                                                                      cAAATTTCTTCTTATTTAATCTGGCGCTGAACTATATTCCACCGACCAGCAGCCAAGTGCTCAGTCCGTTATCCTCGTTTATCATGCTATTTGTCGGTGTGTTTGTGTTTAAAGAACACATCGGGCTCCATCAAAAAATCGGTTTGGTTATTTTAATTCTCAGCTTGGTCTTATTTTTCAACCAACGCTTTAGTGATTTCTTACAGCTCAATGATT‑ATTTTAAAGGCGTTGTTATTGCCCTATGCGCCAg                                                                                                                                                         <  1:590495/250‑1 (MQ=255)                                                                                                  tATTTAATCTGGCGCTGAACTATATTCCACCGACCAGCAGCCAAGTGCTCAGTCCGTTATCCTCGTTTATCATGCTATTTGTCGGTGTGTTTGTGTTTAAAGAACACATCGGGCTCCATCAAAAAATCGGTTTGGTTATTTTAATTCTCAGCTTGGTCTTATTTTTCAACCAACGCTTTAGTGATTTCTTACAGCTCAATGATT‑ATTTTAAAGGCGTTGTTATTGCCCTATGCGCCAGCTCctgctg                                                                                                                                                <  1:153944/247‑1 (MQ=255)                                                                                                   aTTTAATCTGGCGCTGAACTATATTCCACCGACCAGCAGCCAAGTGCTCAGTCCGTTATCCTCGTTTATCATGCTATTTGTCGGTGTGTTTGTGTTTAAAGAACACATCGGGCTCCATCAAAAAATCGGTTTGGTTATTTTAATTCTCAGCTTGGTCTTATTTTTCAACCAACGCTTTAGTGATTTCTTACAGCTCAATGATT‑ATTTTAAAGGCGTTGTTATTGccc                                                                                                                                                                   >  1:520551/1‑227 (MQ=255)                                                                                                   aTTTAATCTGGCGCTGAACTATATTCCACCGACCAGCAGCCAAGTGCTCAGTCCGTTATCCTCGTTTATCATGCTATTTGTCGGTGTGTTTGTGTTTAAAGAACACATCGGGCTCCATCAAAAAATCGGTTTGGTTATTTTAATTCTCAGCTTGGTCTTATTTTTCAACCAACGCTTTAGTGATTTCTTACAGCTCAATGATT‑ATTTTAAAGGCGTTGTTATTGccc                                                                                                                                                                   >  1:409748/1‑227 (MQ=255)                                                                                                        aTCTGGCGCTGAACTATATTCCACCGACCAGCAGCCAAGTGCTCAGTCCGTTATCCTCGTTTATCATGCTATTTGTCGGTGTGTTTGTGTTTAAAGAACACATCGGGCTCCATCAAAAAATCGGTTTGGTTATTTTAATTCTCAGCTTGGTCTTATTTTTCAACCAACGCTTTAGTGATTTCTTACAGCTCAATGATT‑ATTTTAAAGGCGTTGTTATTGCCCTATGCGCCAGCTCCTGCTGGGTTGCCTat                                                                                                                                      <  1:576831/251‑1 (MQ=255)                                                                                                        aTCTGGCGCTGAACTATATTCCACCGACCAGCAGCCAAGTGCTCAGTCCGTTATCCTCGTTTATCATGCTATTTGTCGGTGTGTTTGTGTTTAAAGAACACATCGGGCTCCATCAAAAAATCGGTTTGGTTATTTTAATTCTCAGCTTGGTCTTATTTTTCAACCAACGCTTTAGTGATTTCTTACAGCTCAATGATT‑ATTTTAAAGGCGTTGTTATTGCCCTATGCGCCAGCTCCTGCTGGGTTGCCTat                                                                                                                                      <  1:475632/251‑1 (MQ=255)                                                                                                           tGGCGCTGAACTATATTCCACCGACCAGCAGCCAAGTGCTCAGTCCGTTATCCTCGTTTATCATGCTATTTGTCGGTGTGTTTGTGTTTAAAGAACACATCGGGCTCCATCAAAAAATCGGTTTGGTTATTTTAATTCTCAGCTTGGTCTTATTTTTCAACCAACGCTTTAGTGATTTCTTACAGCTCAATGATT‑ATTTTAAAGGCGTTGTTATTGCCCTATGCGCCAGCTCCTGCTGGGTTGCCTatggc                                                                                                                                   <  1:122711/251‑1 (MQ=255)                                                                                                                  gAACTATATTCCTCCGACCAGCAGCCAAGTGCTCAGTCCGTTATCCTCGTTTATCATGCTATTTGTCGGTGTGTTTGTGTTTAAAGAACACATCGGGCTCCATCAAAAAATCGGTTTGGTTATTTTAATTCTCAGCTTGGTCTTATTTTTcaac                                                                                                                                                                                                                              <  1:115724/154‑1 (MQ=255)                                                                                                                  gAACTATATTCCACCGACCAGCAGCCAAGTGCTCAGTCCGTTATCCTCGTTTATCATGCTATTTGTCGGTGTGTTTGTGTTTAAAGAACACATCGGGCTCCATCAAAAAATCGGTTTGGTTATTTTAATTCTCAGCt                                                                                                                                                                                                                                               >  1:66144/1‑137 (MQ=255)                                                                                                                  gAACTATATTCCACCGACCAGCAGCCAAGTGCTCAGTCCGTTATCCTCGTTTATCATGCTATTTGTCGGTGTGTTTGTGTTTAAAGAACACATCGGGCTCCATCAAAAAATCGGTTTGGTTATTTTAATTCTCAGCTTGGTCTTATTTTTCAACCAACGCTTTAGTGa                                                                                                                                                                                                                >  1:274593/1‑168 (MQ=255)                                                                                                                  gAACTATATTCCACCGACCAGCAGCCAAGTGCTCAGTCCGTTATCCTCGTTTATCATGCTATTTGTCGGTGTGTTTGTGTTTAAAGAACACATCGGGCTCCATCAAAAAATCGGTTTGGTTATTTTAATTCTCAGCTTGGTCTTATTTTTCAACCAACGCTTTAGTGATTTCTTACAGCTCAATGATT‑ATTTTAAAGGCGTTGTTATTGccc                                                                                                                                                                   <  1:86025/212‑1 (MQ=255)                                                                                                                  gAACTATATTCCACCGACCAGCAGCCAAGTGCTCAGTCCGTTATCCTCGTTTATCATGCTATTTGTCGGTGTGTTTGTGTTTAAAGAACACATCGGGCTCCATCAAAAAATCGGTTTGGTTATTTTAATTCTCAGCTTGGTCTTATTTTTCAACCAACGCTTTAGTGATTTCTTACAGCTCAATGATT‑ATTTTAAAGGCGTTGTTATTGCCCTATGCGCCAGCTCCTGCTGGGTTGCCTATGGCATGgcg                                                                                                                             >  1:344826/1‑250 (MQ=255)                                                                                                                    aCTATATTCCACCGACCAGCAGCCAAGTGCTCAGTCCGTTATCCTCGTTTATCATGCTATTTGTCGGTGTGTTTGTGTTTAAAGAACACATCGGGCTCCATCAAAAAATCGGTTTGGTTATTTTAATTCTCAGCTTGGTCTTATTTTTCAACCAACGCTTTAGTGATTTCTTAc                                                                                                                                                                                                        <  1:344436/174‑1 (MQ=255)                                                                                                                     cTATATTCCACCGACCAGCAGCCAAGTGCTCAGTCCGTTATCCTCGTTTATCATGCTATTTGTCGGTGTGTTTGTGTTTAAAGAACACATCGGGCTCCATCAAAAAATCGGTTTGGTTATTTTAATTCTCAGCTTGGTCTTATTTTTCAACCAACGCTTTAGTGATTTCTTACAGCTCAATGATT‑ATTTTAAAGGCGTTGTTATTGCCCTATGCGCCAGCTCCTGCTGGGTTGCCTatggc                                                                                                                                   >  1:745967/1‑241 (MQ=255)                                                                                                                       atatTCCACCGACCAGCAGCCAAGTGCTCAGTCCGTTATCCTCGTTTATCATGCTATTTGTCGGTGTGTTTGTGTTTAAAGAACACATCGGGCTCCATCAAAAAATCGGTTTGGTTATTTTAATTCTCAGCTTGGTCTTATTTTTCAACCAACGCTTTAGTGATTTCTTACAGCTCAATGa                                                                                                                                                                                              >  1:116568/1‑181 (MQ=255)                                                                                                                       atatTCCACCGACCAGCAGCCAAGTGCTCAGTCCGTTATCCTCGTTTATCATGCTATTTGTCGGTGTGTTTGTGTTTAAAGAACACATCGGGCTCCATCAAAAAATCGGTTTGGTTATTTTAATTCTCAGCTTGGTCTTATTTTTCAACCAACGCTTTAGTGATTTCTTACAGCTCAATGATT‑ATTTTAAAGGCGTTGTTa                                                                                                                                                                         >  1:508299/1‑201 (MQ=255)                                                                                                                       atatTCCACCGACCAGCAGCCAAGTGCTCAGTCCGTTATCCTCGTTTATCATGCTATTTGTCGGTGTGTTTGTGTTTAAAGAACACATCGGGCTCCATCAAAAAATCGGTTTGGTTATTTTAATTCTCAGCTTGGTCTTATTTTTCAACCAACGCTTTAGTGATTTCTTACAGCTCAATGATT‑ATTTTAAAGGCGTTGTTATTGCCCTATGCGCCAGCTCctgct                                                                                                                                                 >  1:315182/1‑225 (MQ=255)                                                                                                                       atatTCCACCGACCAGCAGCCAAGTGCTCAGTCCGTTATCCTCGTTTATCATGCTATTTGTCGGTGTGTTTGTGTTTAAAGAACACATCGGGCTCCATCAAAAAATCGGTTTGGTTATTTTAATTATCAGCTTGGTCTTATTTTTCAACCAACGCTTTAGTGATTTCTTACAGCTCAATGATT‑ATTTTAAAGGCGTTGTTATTGCCCTATGCGCCAGCTCCTGCTGGGTTGCCTATGGCATGGCGCAAAAg                                                                                                                       >  1:812515/1‑251 (MQ=255)                                                                                                                              aCCGACCAGCAGCCAAGTGCTCAGTCCGTTATCCTCGTTTATCATGCTATTTGTCGGTGTGTTTGTGTTTAAAGAACACATCGGGCTCCATCAAAAAATCGGTTTGGTTATTTTAATTCTCAGCTTGGTCTTATTTTTCAACCAACGCTTTAGTGATTTCTTACAGCTCAATGATT‑ATTTTAAAGGCGTTGTTATTGCCCTATGCGCCAGCTCCTGCTGGGTTGCCTATGGCATGGCGCAAAAGCTGATGt                                                                                                                <  1:220528/251‑1 (MQ=255)                                                                                                                                   ccAGCAGCCAAGTGCTCAGTCCGTTATCCTCGTTTATCATGCTATTTGTCGGTGTGTTTGTGTTTAAAGAACACATCGGGCTCCATCAAAAAATCGGTTTGGTTATTTTAATTCTCAGCTTGGTCTTATTTTTCAACCAACGCTTTAGTGATTTCTTACAGCTCAATGATT‑ATTTTAAAGGCGTTGTTATTGCCCTATGCGCCAGCTCCTGCTGGGTTGCCTATGGCATGGCGCAAAAGCTGATGTTGAGc                                                                                                           <  1:357938/251‑1 (MQ=255)                                                                                                                                         gcCAAGTGCTCAGTCCGTTATCCTCGTTTATCATGCTATTTGTCGGTGTGTTTGTGTTTAAAGAACACATCGGGCTCCATCAAAAAATCGGTTTGGTTATTTTAATTCTCAGCTTGGTCTTATTTTTCAACCAACGCTTTAGTGATTTCTTACAGCTCAATGa                                                                                                                                                                                              >  1:819869/1‑163 (MQ=255)                                                                                                                                         gcCAAGTGCTCAGTCCGTTATCCTCGTTTATCATGCTATTTGTCGGTGTGTTTGTGTTTAAAGAACACATCGGGCTCCATCAAAAAATCGGTTTGGTTATTTTAATTCTCAGCTTGGTCTTATTTTTCAACCAACGCTTTAGTGATTTCTTACAGCTCAATGATT‑ATTTTAAAGGCGTTGTTATTGCCCTATGCGCCAGCTCCTGCTGGGTTGCCTATGGCATGGCGCAAAAGCTGATGTTGAGCCGttt                                                                                                      >  1:302326/1‑250 (MQ=255)                                                                                                                                              gTGCTCAGTCCGTTATCCTCGTTTATCATGCTATTTGTCGGTGTGTTTGTGTTTAAAGAACACATCGGGCTCCATCAAAAAATCGGTTTGGTTATTTTAATTCTCAGCTTGGTCTTATTTTTCAACCAACGCTTTAGTGATTTCTTACAGCTCAATGATT‑ATTTTAAAGGCGTTGTTATTGCCCTATGCGCCAGCTCCTGCTGGGTTGCCTATGGCATGGCGCAAAAGCTGATGTTGAGCCGTTTCAGtt                                                                                                 >  1:503964/1‑250 (MQ=255)                                                                                                                                                gCTCAGTCCGTTATCCTCGTTTATCATGCTATTTGTCGGTGTGTTTGTGTTTAAAGAACACATCGGGCTCCATCAAAAAATCGGTTTGGTTATTTTAATTCTCAGCTTGGTCTTATTTTTCAACCAACGCTTTAGTGATTTCt                                                                                                                                                                                                           >  1:22774/1‑143 (MQ=255)                                                                                                                                                gCTCAGTCCGTTATCCTCGTTTATCATGCTATTTGTCGGTGTGTTTGTGTTTAAAGAACACATCGGGCTCCATCAAAAAATCGGTTTGGTTATTTTAATTCTCAGCTTGGTCTTATTTTTCAACCAACGCTTTAGTGATTTCTTACAGCTCAATGa                                                                                                                                                                                              >  1:435237/1‑156 (MQ=255)                                                                                                                                                gCTCAGTCCGTTATCCTCGTTTATCATGCTATTTGTCGGTGTGTTTGTGTTTAAAGAACACATCGGGCTCCATCAAAAAATCGGTTTGGTTATTTTAATTCTCAGCTTGGTCTTATTTTTCAACCAACGCTTTAGTGATTTCTTACAGCTCAATGATT‑ATTTTAAAGGCGTTGTTATTGccc                                                                                                                                                                   <  1:766907/182‑1 (MQ=255)                                                                                                                                                gCTCAGTCCGTTATCCTCGTTTATCATGCTATTTGTCGGTGTGTTTGTGTTTAAAGAACACATCGGGCTCCATCAAAAAATCGGTTTGGTTATTTTAATTCTCAGCTTGGTCTTATTTTTCAACCAACGCTTTAGTGATTTCTTACAGCTCAATGATT‑ATTTTAAAGGCGTTGTTATTGCCCTATGCGCCAGCTCCTGCTGGGTTGCCTATGGCATGGCGCAAAAGCTGATGt                                                                                                                <  1:267034/233‑1 (MQ=255)                                                                                                                                                       ccGTTATCCTCGTTTATCATGCTATTTGTCGGTGTGTTTGTGTTTAAAGAACACATCGGGCTCCATCAAAAAATCGGTTTGGTTATTTTAATTCTCAGCTTGGTCTTATTTTTCAACCAACGCTTTAGTGATTTCTTACAGCTCAATGATT‑ATTTTAAAGGCGTTGTTa                                                                                                                                                                         <  1:880491/169‑1 (MQ=255)                                                                                                                                                       ccGTTATCCTCGTTTATCATGCTATTTGTCGGTGTGTTTGTGTTTAAAGAACACATCGGGCTCCATCAAAAAATCGGTTTGGTTATTTTAATTCTCAGCTTGGTCTTATTTTTCAACCAACGCTTTAGTGATTTCTTACAGCTCAATGATT‑ATTTTAAAGGCGTTGTTATTGCCCTATGCGCCAGCTCCTGCTGGGTTGCCTatggc                                                                                                                                   >  1:127257/1‑207 (MQ=255)                                                                                                                                                        cGTTATCCTCGTTTATCATGCTATTTGTCGGTGTGTTTGTGTTTAAAGAACACATCGGGCTCCATCAAAAAATCGGTTTGGTTATTTTAATTCTCAGCTTGGTCTTATTTTTCAACCAACGCTTTAGTGATTTCTTACAGCTCAATGATT‑ATTTTAAAGGCGTTGTTATTGCCCTATGCGCCAGCTCCTGCTGGGTTGCCTATGGCATGGCGCAAAAGCTGATGTTGAGCCGTTTCAGTTCGCAACAAAtt                                                                                      <  1:517420/251‑1 (MQ=255)                                                                                                                                                                 cGTTTATCATGCTATTTGTCGGTGTGTTTGTGTTTAAAGAACACATCGGGCTCCATCAAAAAATCGGTTTGGTTATTTTAATTCTCAGCTTGGTCTTATTTTTCAACCAACGCTTTAGTGATTTCTTACAGCTCAATGATT‑ATTTTAAAGGCGTTGTTATTGCCCTATGCGCCAGCTCCTGCTGGGTTGCCTATGGCATGGCGCAAAAGCTGATGTTGAGCCGTTTCAGTTCGCAACAAATTTTGCTCATa                                                                             >  1:150795/1‑251 (MQ=255)                                                                                                                                                                      aTCATGCTATTTGTCGGTGTGTTTGTGTTTAAAGAACACATCGGGCTCCATCAAAAAATCGGTTTGGTTATTTTAATTCTCAGCTTGGTCTTATTTTTCAACCAACGCTTTAGTGATTTCTTACAGCTCAATGATT‑ATTTTAAAGGCGTTGTTATTGCCCTATGCGCCAGCTCCTGCTGGGTTGCCTATGGCATGGCGCAAAAGCTGATGTTGAGCCGTTTCAGTTCGCAACAAAt                                                                                       <  1:62497/236‑1 (MQ=255)                                                                                                                                                                          tGCTATTTGTCGGTGTGTTTGTGTTTAAAGAACACATCGGGCTCCATCAAAAAATCGGTTTGGTTATTTTAATTCTCAGCTTGGTCTTATTTTTCAACCAACGCTTTAGTGATTTCTTACAGCTCAATGATT‑ATTTTAAAGGCGTTGTTATTGCCCTATGCGCCAGCTCCTGCTGGGTTGCCTATGGCATGGCGCAAAAGCTGATGTTGAGCCGTTTCAGTTCGCAACAAATTTTGCTCATAATTTATGt                                                                     >  1:391386/1‑250 (MQ=255)                                                                                                                                                                           gCTATTTGTCGGTGTGTTTGTGTTTAAAGAACACATCGGGCTCCATCAAAAAATCGGTTTGGTTATTTTAATTCTCAGCTTGGTCTTATTTTTCAACCAACGCTTTAGTGATTTCTTACAGCTCAATGATT‑ATTTTAAAGGCGTTGTTATTGCCCTATGCGCCAGCTCCTGCTGGGTTGCCTATGGCATGGCGCAAAAGCTGATGTTGAGCCGTTTCAGTTCGCAACAAATTTTGCTCATAATTTATGTCg                                                                   >  1:866435/1‑251 (MQ=255)                                                                                                                                                                           gCTATTTGTCGGTGTGTTTGTGTTTAAAGAACACATCGGGCTCCATCAAAAAATCGGTTTGGTTATTTTAATTCTCAGCTTGGCCTTATTTTTCAACCAACGCTTTAGTGATTTCTTACAGCTCAATGATT‑ATTTTAAAGGCGTTGTTATTGCCCTATGCGCCAGCTCCTGCTGGGTTGCCTATGGCATGGCGCAAAAGCTGATGTTGAGCCGTTTCAGTTCGCAACAAATTTTGCTCATAATTTATGTCg                                                                   >  1:444065/1‑251 (MQ=255)                                                                                                                                                                            cTATTTGTCGGTGTGTTTGTGTTTAAAGAACACATCGGGCTCCATCAAAAAATCGGTTTGGTTATTTTAATTCTCAGCTTGGTCTTATTTTTCAACCAACGCTTTAGTGATTTCTTACAGCTCAATGATT‑ATTTTAAAGGCGTTGTTATTGCCCTATGCGCCAGCt                                                                                                                                                       >  1:577327/1‑166 (MQ=255)                                                                                                                                                                            cTATTTGTCGGTGTGTTTGTGTTTAAAGAACACATCGGGCTCCATCAAAAAATCGGTTTGGTTATTTTAATTCTCAGCTTGGTCTTATTTTTCAACCAACGCTTTAGTGATTTCTTACAGCTCAATGATT‑ATTTTAAAGGCGTTGTTATTGCCCTATGCGCCAGCTCCTGCTGGGTTGCCTATGGCATGGCGCAAAAGCTGATGTTGAGCCGTTTCAGTTCGCAACAAATTTTGCTCATAATTTATGTCgg                                                                  >  1:274313/1‑251 (MQ=255)                                                                                                                                                                            cTATTTGTCGGTGTGTTTGTGTTTAAAGAACACATCGGGCTCCATCAAAAAATCGGTTTGGTTATTTTAATTCTCAGCTTGGTCTTATTTTTCAACCAACGCTTTAGTGATTTCTTACAGCTCAATGATT‑ATTTTAAAGGCGTTGTTATTGCCCTATGCGCCAGCTCCTGCTGGGTTGCCTATGGCATGGCGCAAAAGCTGATGTTGAGCCGTTTCAGTTCGCAACAAATTTTGCTCATAATTTATGTCg                                                                   <  1:525131/250‑1 (MQ=255)                                                                                                                                                                              aTTTGTCGGTGTGTTTGTGTTTAAAGAACACATCGGGCTCCATCAAAAAATCGGTTTGGTTATTTTAATTCTCAGCTTGGTCTTATTTTTCAACCAACGCTTTAGTGATTTCTTACAGCTCAATGATT‑ATTTTAAAGGCGTTGTTATTGccc                                                                                                                                                                   >  1:239518/1‑152 (MQ=255)                                                                                                                                                                                ttGTCGGTGTGTTTGTGTTTAAAGAACACATCGGGCTCCATCAAAAAATCGGTTTGGTTATTTTAATTCTCAGCTTGGTCTTATTTTTCAACCAACGCTTTAGTGATTTCTTACAGCTCAATGATT‑ATTTTAAAGGCGTTGTTATTGCCCTATGCGCCAGCTCCTGCTGGGTTGCCTATGGCATGGCGCAAAAGCTGATGTTGAGCCGTTTCAGTTCGCAACAAATTTTGCTCATAATTTATGTCGGCTg                                                               >  1:721678/1‑250 (MQ=255)                                                                                                                                                                                  gTCGGTGTGTTTGTGTTTAAAGAACACATCGGGCTCCATCAAAAAATCGGTTTGGTTATTTTAATTCTCAGCTTGGTCTTATTTTTCAACCAACGCTTTAGTGATTTCTTACAGCTCAATGATT‑ATTTTAAAgg                                                                                                                                                                                 <  1:786357/134‑1 (MQ=255)                                                                                                                                                                                            ttgtgtttAAAGAACACATCGGGCTCCATCAAAAAATCGGTTTGGTTATTTTAATTCTCAGCTTGGTCTTATTTTTCAACCAACGCTTTAGTGATTTCTTACAGCTCAATGATT‑ATTTTAAAGGCGTTGTTATTGCCCTATGCGCCAGCTCCTGCTGGGTTGCCTATGGCATGGCGCAAAAGCTGATGTTGAGCCGTTTCAGTTCGCAACAAATTTTGCTCATAATTTATGTCGGCTGCACGATCAttttt                                                  <  1:308907/251‑1 (MQ=255)                                                                                                                                                                                                       gAACACATCGGGCTCCATCAAAAAATCGGTTTGGTTATTTTAATTCTCAGCTTGGTCTTATTTTTCAACCAACGCTTTAGTGATTTCTTACAGCTCAATGATT‑ATTTTAAAGGCGTTGTTATTGCCCTATGCGCCAGCTCCTGCTGGGTTGCCTATGGCATGGCGCAAAAGCTGATGTTGAGCCGTTTCAGTTCGCAACAAATTTTGCTCATAATTTATGTCGGCTGCACGATCATTTTTACGCCCTTTg                                        >  1:600999/1‑250 (MQ=255)                                                                                                                                                                                                           acaTCGGGCTCCATCAAAAAATCGGTTTGGTTATTTTAATTCTCAGCTTGGTCTTAttt                                                                                                                                                                                                                                    >  1:709662/1‑59 (MQ=255)                                                                                                                                                                                                            caTCGGGCTCCATCAAAAAATCGGTTTGGTTATTTTAATTCTCAGCTTGGTCTTATTTTTCAACCAACGCTTTAGTGATTTCTTACAGCTCAATGATTAATTTTAAAGGCGTTGTTATTGCCCTATGCGCCAGCTCCTGCTGGGTTGCCTATGGCATGGCGCAAAAGCTGATGTTGAGCCGTTTCAGTTCGCAACAAATTTTGCTCATAATTTATGTCGGCTGCACGATCATTTTTACGCCCTTTGCCAat                                   >  1:458838/1‑251 (MQ=255)                                                                                                                                                                                                                  gCTCCATCAAAAAATCGGTTTGGTTATTTTAATTCTCAGCTTGGTCTTATTTTTCAACCAACGCTTTAGTGATTTCTTACAGCTCAATGATT‑ATTTTAAAGGCGTTGTTATTGCCCTATGCGCCAGCTCCTGCTGGGTTGCCTATGGCATGGCGCAAAAGCTGATGTTGAGCCGTTTCAGTTCGCAACAAATTTTGCTCATAATTTATGTCGGCTGCACGATCATTTTTACGCCCTTTGCCAATATTcag                             <  1:253467/250‑1 (MQ=255)                                                                                                                                                                                                                      cATCAAAAAATCGGTTTGGTTATTTTAATTCTCAGCTTGGTCTTATTTTTCAACCAACGCTTTAGTGATTTCTTACAGCTCAATGATT‑ATTTTAAAGGCGTTGTTATTGCCCTATGCGCCAGCTCCTGCTGGGTTGCCTATGGCATGGCGCAAAAGCTGATGTTGAGCCGTTTCAGTTc                                                                                                >  1:495372/1‑179 (MQ=255)                                                                                                                                                                                                                       aTCAAAAAATCGGTTTGGTTATTTTAATTCTCAGCTTGGTCTTATTTTTCAACCAACGCTTTAGTGATTTCTTACAGCTCAATGATT‑ATTTTAAAGGCGTTGTTATTGCCCTATGCGCCAGCTCCTGCTGGGTTGCCTATGGCATGGCGCAAAAGCTGATGTTGAGCCGTTTCAGTTCGCAACAAATTTTGCTCATAATTTATGTCGGCTGCACGATCATTTTTACGCCCTTTGCCAATATTCAGCAGGTg                       <  1:723204/251‑1 (MQ=255)                                                                                                                                                                                                                       aTCAAAAAATCGGTTTGGTTATTTTAATTCTCAGCTTGGTCTTATTTTTCAACCAACGCTTTAGTGATTTCTTACAGCTCAATGATT‑ATTTTAAAGGCGTTGTTATTGCCCTATGCGCCAGCTCCTGCTGGGTTGCCTATGGCATGGCGCAAAAGCTGATGTTGAGCCGTTTCAGTTCGCAACAAATTTTGCTCATAATTTATGTCGGCTGCACGATCATTTTTACGCCCTTTGCCAATATTCAGCAGGTg                       <  1:667746/251‑1 (MQ=255)                                                                                                                                                                                                                       aTCAAAAAATCGGTTTGGTTATTTTAATTCTCAGCTTGGTCTTATTTTTCAACCAACGCTTTAGTGATTTCTTACAGCTCAATGATT‑ATTTTAAAGGCGTTGTTATTGCCCTATGCGCCAGCTCCTGCTGGGTTGCCTATGGCATGGCGCAAAAGCTGATGTTGAGCCGTTTCAGTTCGCAACAAATTTTGCTCATAATTTATGTCGGCTGCACGATCATTTTTACGCCCTTTGCCAATATTCAGCAGGTg                       <  1:885342/251‑1 (MQ=255)                                                                                                                                                                                                                        tCAAAAAATCGGTTTGGTTATTTTAATTCTCAGCTTGGTCTTATTTTTCAACCAACGCTTTAGTGATTTCTTACAGCTCAATGa                                                                                                                                                                                              <  1:597408/84‑1 (MQ=255)                                                                                                                                                                                                                         cAAAAAATCGGTTTGGTTATTTTAATTCTCAGCTTGGTCTTATTTTTCAACCAACGCTTTAGTGATTTCTTACAGCTCAATGATT‑ATTTTAAAGGCGTTGTTATTGCCCTATGCGCCAGCTCCTGCTGGGTTGCCTATGGCATGGCGCAAAAGCTGATGTTGAGCCGTTTCAGTTCGCAACAAATTTTGCTCATAATTTATGTCGGCTGCACGATCATTTTTACGCCCTTTGCCAATATTCAGCAGGTGAg                     <  1:536901/251‑1 (MQ=255)                                                                                                                                                                                                                           aaaaaTCGGTTTGGTTATTTTAATTCTCAGCTTGGTCTTATTTTTCAACCAACGCTTTAGTGATTTCTTACAGCTCAATGATT‑ATTTTAAAGGCGTTGTTATTGCCCTATGCGCCAGCTCCTGCTGGGTTGCCTATGGCATGGCGCAAAAGCTGATGTTGAGCCGTTTCAGTTCGCAACAAATTTTGCTCATAATTTATGTCGGCTGCACGATCATTTTTACGCCCTTTGCCAATATTCAGCAGGTg                       >  1:611780/1‑247 (MQ=255)                                                                                                                                                                                                                            aaaaTCGGTTTGGTTATTTTAATTCTCAGCTTGGTCTTATTTTTCAACCAACGCTTTAGTGATTTCTTACAGCTCAATGATT‑ATTTTAAAGGCGTTGTTATTGCCCTATGCGCCAGCTCCTGCTGGGt                                                                                                                                             >  1:340237/1‑128 (MQ=255)                                                                                                                                                                                                                             aaaTCGGTTTGGTTATTTTAATTCTCAGCTTGGTCTTATTTTTCAACCAACGCTTTAGTGATTTCTTACAGCTCAATGATT‑ATTTTAAAGGCGTTGTTa                                                                                                                                                                         <  1:627209/99‑1 (MQ=255)                                                                                                                                                                                                                                     ttGGTTATTTTAATTCTCAGCTTGGTCTTATTTTTCAATCAACACTTTAGTGATTTCTTACAGCTCAATGATT‑ATTTTAAAGGCGTTGTTATTGCCCTATGCGCCAGCTCCTGCTGGGTTGCCTATGGCATGGCGCAAAAGCTGATGTTGAGCCGTTTCAGTTCGCAACAAATTTTGCTCATAATTTATGTCGGCTGCACGATCATTTTTACGCCCTTTGCCAATATTCAGCAGGTGAGCGCGTTAAATTc         <  1:185879/251‑1 (MQ=255)                                                                                                                                                                                                                                            ttttAATTCTCAGCTTGGTCTTATTTTTCAACCAACGCTTTAGTGATTTCTTACAGCTCAATGATT‑ATTTTAAAGGCGTTGTTATTGCCCTATGCGCCAGCTCCTGCTGGGTTGCCTATGGCATGGCGCAAAAGCTGATGTTGAGCCGTTTCAGTTCGCAACAAATTTTGCTCATAATTTATGTCGGCTGCACGATCATTTTTACGCCCTTTGCCAATATTTAGCAGGTGAGCGCGTTAAATTCCTTTCAg  >  1:269218/1‑251 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                 GGATTACGTAAAACGCTGCCAAAACCCACCGCACTTCGTCAGCGTTATCTCCTTTTGATTATGCTGGGCATTCTTGGGCTATCGGCAAATTTCTTCTTATTTAATCTGGCGCTGAACTATATTCCACCGACCAGCAGCCAAGTGCTCAGTCCGTTATCCTCGTTTATCATGCTATTTGTCGGTGTGTTTGTGTTTAAAGAACACATCGGGCTCCATCAAAAAATCGGTTTGGTTATTTTAATTCTCGGCTTGGTCTTATTTTTCAACCAACGCTTTAGTGATTTCTTACAGCTCAATGATT‑ATTTTAAAGGCGTTGTTATTGCCCTATGCGCCAGCTCCTGCTGGGTTGCCTATGGCATGGCGCAAAAGCTGATGTTGAGCCGTTTCAGTTCGCAACAAATTTTGCTCATAATTTATGTCGGCTGCACGATCATTTTTACGCCCTTTGCCAATATTCAGCAGGTGAGCGCGTTAAATTCCTTTCAG  >  1/2027151‑2027636

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑