Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	2,021,404	A→C	S99R (AGC→CGC)	unknown →	Primosomal replication protein N''

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	2,021,404	0	A	C	97.7%	120.0 / ‑3.8	44	coding (295/567 nt)	unknown	Primosomal replication protein N''
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base C (33/10);  minor base . (0/1);  total (33/11)
Fisher's exact test for biased strand distribution p-value = 2.50e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGCGTTATGACGCGCAAAAGGAAGGAACGGTGTATGCCAAATTTTCGGCAAATTTATTTACGGAAAACCGGCAAAAAGTAGCATTTTATTTTCAGCAAATCGAACAAACCTTTGCGCGTCTTGAGCAGGCGGATCCCAGCAATCTTGAAGCGTTGCAGTTTTACACGCAAAAACTGTCGGCGCAATGCACCGCACTTTCCGATGCCTTAACCCGACAACAGCAAAACGACCAACCCTTTCCGAGCAAAACC‑AAGGAAGAACCTAAACCCGCCGGTAAACGCCGTCATCCCGTACACAGTTTGCCGCCCCGTGAACGGTTAGCGAAATATTATGACTATCTGGCATCGTTCAATGAAAAAATTCAGGTTGAACAGGATACGTTGGAAAAAGCACAGCGTGAAGGTCGACTTGTTAATAAACAAATGCTGGAGCAATTAGAACAGCGCCGTGCCCGTTGTTTGGAAGCGATTGACGTGCTGGAA  >  1/2021162‑2021643                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                 cgcgTTATGACGCGCAAAAGGAAGGAACGGTGTATGCCAAATTTTCGGCAAATTTATTTACGGAAAACCGGCAAAAAGTAGCATTTTATTTTCAGCAAATCGAACAAACCTTTGCGCGTCTTGAGCAGGCGGATCCCAGCAATCTTGAAGCGTTGCAGTTTTACACGCAAAAACTGTCGGCGCAATGCACCGCACTTTCCGATGCCTTAACCCGACAACAGCAAAACGACCAACCCTTTCCGCGCAAAAc                                                                                                                                                                                                                                           <  1:216500/250‑1 (MQ=255) cgcgTTATGACGCGCAAAAGGAAGGAACGGTGTATGCCAAATTTTCGGCAAATTTATTTACGGAAAACCGGCAAAAAGTAGCATTTTATTTTCAGCAAATCGAACAAACCTTTGCGCGTCTTGAGCAGGCGGATCCCAGCAATCTTGAAGCGTTGCAGTTTTACACGCAAAAACTGTCGGCGCAATGCACCGCACTTTCCGATGCCTTAACCCGACAACAGCAAAACGACCAACCCTTTCCGCGCAAAAc                                                                                                                                                                                                                                           <  1:306763/250‑1 (MQ=255)   cgTTATGACGCGCAAAAGGAAGGAACGGTGTATGCCAAATTTTCGGCAAATTTATTTACGGAAAACCGGCAAAAAGTAGCATTTTATTTTCAGCAAATCGAACAAACCTTTGCGCGTCTTGAGCAGGCGGATCCCAGCAATCTTGAAGCGTTGCAGTTTTACACGCAAAAACTGTCGGCGCAATGCACCGCACTTTCCGATGCCTTAACCCGACAACAGCAAAACGACCAACCCTTTCCGCGCAAAAc                                                                                                                                                                                                                                           >  1:54759/1‑248 (MQ=255)   cgTTATGACGCGCAAAAGGAAGGAACGGTGTATGCCAAATTTTCGGCAAATTTATTTACGGAAAACCGGCAAAAAGTAGCATTTTATTTTCAGCAAATCGAACAAACCTTTGCGCGTCTTGAGCAGGCGGATCCCAGCAATCTTGAAGCGTTGCAGTTTTACACGCAAAAACTGTCGGCGCAATGCACCGCACTTTCCGATGCCTTAACCCGACAACAGCAAAACGACCAACCCTTTCCGCGCAAAACC‑aa                                                                                                                                                                                                                                       >  1:105674/1‑251 (MQ=255)     ttATGACGCGCAAAAGGAAGGAACGGTGTATGCCAAATTTTCGGCAAATTTATTTACGGAAAACCGGCAAAAAGTAGCATTTTATTTTCAGCAAATCGAACAAACCTTTGCGCGTCTTGAGCAGGCGGATCCCAGCAATCTTGAAGCGTTGCAGTTTTACACGCAAAAACTGTCGGCGCAATGCACCGCACTTTCCGATGCCTTAACCCGACAACAGCAAAACGACCAACCCTTTCCGCGCAAAACC‑AAgg                                                                                                                                                                                                                                     <  1:177515/251‑1 (MQ=255)     ttATGACGCGCAAAAGGAAGGAACGGTGTATGCCAAATTTTCGGCAAATTTATTTACGGAAAACCGGCAAAAAGTAGCATTTTATTTTCAGCAAATCGAACAAACCTTTGCGCGTCTTGAGCAGGCGGATCCCAGCAATCTTGAAGCGTTGCAGTTTTACACGCAAAAACTGTCGGCGCAATGCACCGCACTTTCCGATGCCTTAACCCGACAACAGCAAAACGACCAACCCTTTCCGCGCAAAACC‑AAgg                                                                                                                                                                                                                                     <  1:526916/251‑1 (MQ=255)                 aaaGGAAGGAACGGTGTATGCCAAATTTTCGGCAAATTTATTTACGGAAAACCGGCAAAAAGTAGCATTTTATTTTCAGCAAATCGAACAAACCTTTGCGCGTCTTGAGCAGGCGGATCCCAGCAATCTTGAAGCGTTGCAGTTTTACACGCAAAAACTGTCGGCGCAATGCACCGCACTTTCCGATGCCTTAACCCGACAACAGCAAAACGACCAACCCTTTCCGCGCAAAACC‑AAGGAAGAACCTaaa                                                                                                                                                                                                                          >  1:633275/1‑250 (MQ=255)                  aaggaaggAACGGTGTATGCCAAATTTTCGGCAAATTTATTTACGGAAAACCGGCAAAAAGTAGCTTTTTATTTTCAGCAAATCGAACAAACCTTTGCGCGTCTTGAGCAGGCGGATCCCAGCAATCTTGAAGCGTTGCAGTTTTACACGCAAAAACTGTCGGCGCAATGCACCGCACTTTCCGATGCCTTAACCCGACAACAGCAAAACGACCAACCCTTTCCGCGCAAAACC‑AAGGAAGAACCTAAAcc                                                                                                                                                                                                                        <  1:49151/251‑1 (MQ=255)                  aaggaaggAACGGTGTATGCCAAATTTTCGGCAAATTTATTTACGGAAAACCGGCAAAAAGTAGCATTTTATTTTCAGCAAATCGAACAAACCTTTGCGCGTCTTGAGCAGGCGGATCCCAGCAATCTTGAAGCGTTGCAGTTTTACACGCAAAAACTGTCGGCGCAATGCACCGCACTTTCCGATGCCTTAACCCGACAACAGCAAAACGACCAACCCTTTCCGCGCAAAACC‑AAGGAAGAACCTAAAcc                                                                                                                                                                                                                        <  1:878483/251‑1 (MQ=255)                  aaggaaggAACGGTGTATGCCAAATTTTCGGCAAATTTATTTACGGAAAACCGGCAAAAAGTAGCATTTTATTTTCAGCAAATCGAACAAACCTTTGCGCGTCTTGAGCAGGCGGATCCCAGCAATCTTGAAGCGTTGCAGTTTTACACGCAAAAACTGTCGGCGCAATGCACCGCACTTTCCGATGCCTTAACCCGACAACAGCAAAACGACCAACCCTTTCCGCGCAAAACC‑AAGGAAGAACCTAAAcc                                                                                                                                                                                                                        <  1:654058/251‑1 (MQ=255)                  aaggaaggAACGGTGTATGCCAAATTTTCGGCAAATTTATTTACGGAAAACCGGCAAAAAGTAGCATTTTATTTTCAGCAAATCGAACAAACCTTTGCGCGTCTTGAGCAGGCGGATCCCAGCAATCTTGAAGCGTTGCAGTTTTACACGCAAAAACTGTCGGCGCAATGCACCGCACTTTCCGATGCCTTAACCCGACAACAGCAAAACGACCAACCCTTTCCGCGCAAAACC‑AAGGAAGAACCTAAAcc                                                                                                                                                                                                                        <  1:21066/251‑1 (MQ=255)                  aaggaaggAACGGTGTATGCCAAATTTTCGGCAAATTTATTTACGGAAAACCGGCAAAAAGTAGCATTTTATTTTCAGCAAATCGAACAAACCTTTGCGCGTCTTGAGCAGGCGGATCCCAGCAATCTTGAAGCGTTGCAGTTTTACACGCAAAAACTGTCGGCGCAATGCACCGCACTTTCCGATGCCTTAACCCGACAACAGCAAAACGACCAACCCTTTCCGCGCAAAACC‑AAGGAAGAACCTAAAcc                                                                                                                                                                                                                        <  1:214238/251‑1 (MQ=255)                         gaaCGGTGTATGCCAAATTTTCGGCAAATTTATTTACGGAAAACCGGCAAAAAGTAGCATTTTATTTTCAGCAAATCGAACAAACCTTTGCGCGTCTTGAGCAGGCGGATCCCAGCAATCTTGAAGCGTTGCAGTTTTACACGCAAAAACTGTCGGCGCAATGCACCGCACTTTCCGATGCCTTAACCCGACAACAGCAAAACGACCAACCCTTTCCGCGCAAAAc                                                                                                                                                                                                                                           >  1:106761/1‑226 (MQ=255)                         gaaCGGTGTATGCCAAATTTTCGGCAAATTTATTTACGGAAAACCGGCAAAAAGTAGCATTTTATTTTCAGCAAATCGAACAAACCTTTGCGCGTCTTGAGCAGGCGGATCCCAGCAATCTTGAAGCGTTGCAGTTTTACACGCAAAAACTGTCGGCGCAATGCACCGCACTTTCCGATGCCTTAACCCGACAACAGCAAAACGACCAACCCTTTCCGCGCAAAACC‑AAGGAAGAACCTAAACCCGCCGGt                                                                                                                                                                                                                 >  1:357424/1‑251 (MQ=255)                         gaaCGGTGTATGCCAAATTTTCGGCAAATTTATTTACGGAAAACCGGCAAAAAGTAGCATTTTATTTTCAGCAAATCGAACAAACCTTTGCGCGTCTTGAGCAGGCGGATCCCAGCAATCTTGAAGCGTTGCAGTTTTACACGCAAAAACTGTCGGCGCAATGCACCGCACTTTCCGATGCCTTAACCCGACAACAGCAAAACGACCAACCCTTTCCGCGCAAAACC‑AAGGAAGAACCTAAACCCGCCGGt                                                                                                                                                                                                                 >  1:364398/1‑251 (MQ=255)                              gtgtATGCCAAATTTTCGGCAAATTTATTTACGGAAAACCGGCAAAAAGTAGCATTTTATTTTCAGCAAATCGAACAAACCTTTGCGCGTCTTGAGCAGGCGGATCCCAGCAATCTTGAAGCGTTGCAGTTTTACACGCAAAAACTGTCGGCGCAATGCACCGCACTTTCCGATGCCTTAACCCGACAACAGCAAAACGACCAACCCTTTCCGCGCAAAACC‑AAGGAAGAACCTAAACCCGCCGGTAAACg                                                                                                                                                                                                            >  1:276126/1‑251 (MQ=255)                              gtgtATGCCAAATTTTCGGCAAATTTATTTACGGAAAACCGGCAAAAAGTAGCATTTTATTTTCAGCAAATCGAACAAACCTTTGCGCGTCTTGAGCAGGCGGATCCCAGCAATCTTGAAGCGTTGCAGTTTTACACGCAAAAACTGTCGGCGCAATGCACCGCACTTTCCGATGCCTTAACCCGACAACAGCAAAACGACCAACCCTTTCCGCGCAAAACC‑AAGGAAGAACCTAAACCCGCCGGTAAACg                                                                                                                                                                                                            >  1:847988/1‑251 (MQ=255)                              gtgtATGCCAAATTTTCGGCAAATTTATTTACGGAAAACCGGCAAAAAGTAGCATTTTATTTTCAGCAAATCGAACAAACCTTTGCGCGTCTTGAGCAGGCGGATCCCAGCAATCTTGAAGCGTTGCAGTTTTACACGCAAAAACTGTCGGCGCAATGCACCGCACTTTCCGATGCCTTAACCCGACAACAGCAAAACGACCAACCCTTTCCGCGCAAAACC‑AAGGAAGAACCTAAACCCGCCGGTAAACg                                                                                                                                                                                                            >  1:523153/1‑251 (MQ=255)                              gtgtATGCCAAATTTTCGGCAAATTTATTTACGGAAAACCGGCAAAAAGTAGCATTTTATTTTCAGCAAATCGAACAAACCTTTGCGCGTCTTGAGCAGGCGGATCCCAGCAATCTTGAAGCGTTGCAGTTTTACACGCAAAAACTGTCGGCGCAATGCACCGCACTTTCCGATGCCTTAACCCGACAACAGCAAAACGACCAACCCTTTCCGCGCAAAACC‑AAGGAAGAACCTAAACCCGCCGGTAAACg                                                                                                                                                                                                            >  1:541443/1‑251 (MQ=255)                                                             cGGAAAACCGGCAAAAAGTAGCATTTTATTTTCAGCAAATCGAACAAACCTTTGCGCGTCTTGAGCAGGCGGATCCCAGCAATCTTGAAGCGTTGCAGTTTTACACGCAAAAACTGTCGGCGCAATGCACCGCACTTTCCGATGCCTTAACCCGACAACAGCAAAACGACCAACCCTTTCCGCGCAAAACC‑AAGGAAGAACCTAAACCCGCCGGTAAACGCCGTCATCCCGTACACAGTTTGCCGCCCCGt                                                                                                                                                                             >  1:414281/1‑251 (MQ=255)                                                                                    ttttattttCAGCAAATCGAACAAACCTTTGCGCGTCTTGAGCAGGCGGATCCCAGCAATCTTGAAGCGTTGCAGTTTTACACGCAAAAACTGTCGGCGCAATGCACCGCACTTTCCGATGCCTTAACCCGACAACAGCAAAACGACCAACCCTTTCCGCGCAAAACC‑AAGGAAGAACCTAAACCCGCCGGTAAACGCCGTCATCCCGTac                                                                                                                                                                                              >  1:780345/1‑211 (MQ=255)                                                                                               aaaaaTCGAACAACCCTTTGCGCGTCTGGAGCGGGCGCCCCCCAACAATATGGAAGCGTTGCATTTTCACACACAAAACCGGCCGGCGCAACCCCCCCCCCTTCCCGATGCCTAACCCCAAAAACAAAAACCCGCCCACCCCTCCCCG‑CAAAAACCAAAGGAAAAACAAAACCCCGCCGGTAAACGCCGTCATCCCGTACACAGTTTGCCGCCCCGTGAACGGTTAGCGAAATATTATGACTATCTGGCAt                                                                                                                                           <  1:442500/249‑1 (MQ=255)                                                                                                      gAACAAACCTTTGCGCGTCTTGAGCAGGCGGATCCCAGCAATCTTGAAGCGTTGCAGTTTTACACGCAAAAACTGTCGGCGCAATGCACCGCACTTTCCGATGCCTTAACCCGACAACAGCAAAACGACCAACCCTTTCCGCGCAAAACC‑AAGGAAGAACCTAAACCCGCCGGTAAACGCCGTCATCCCGTacac                                                                                                                                                                                            >  1:181787/1‑195 (MQ=255)                                                                                                      gAACAAACCTTTGCGCGTCTTGAGCAGGCGGATCCCAGCAATCTTGAAGCGTTGCAGTTTTACACGCAAAAACTGTCGGCGCAATGCACCGCACTTTCCGATGCCTTAACCCGACAACAGCAAAACGACCAACCCTTTCCGCGCAAAACC‑AAGGAAGAACCTAAACCCGCCGGTAAACGCCGTCATCCCGTACACAGTTTGCCGCCCCGTGAACGGTTAGCGAAATATTATGACTATCTGGCATCGTTc                                                                                                                                      >  1:74257/1‑249 (MQ=255)                                                                                                             ccTTTGCGCGTCTTGAGCAGGCGGATCCCAGCAATCTTGAAGCGTTGCAGTTTTACACGCAAAAACTGTCGGCGCAATGCACCGCACTTTCCGATGCCTTAACCCGACAACAGCAAAACGACCAACCCTTTCCGCGCAAAACC‑AAGGAAGAACCTAAACCCGCCGGTAAACGCCGTCATCCCGTacac                                                                                                                                                                                            >  1:282837/1‑188 (MQ=255)                                                                                                             ccTTTGCGCGTCTTGAGCAGGCGGATCCCAGCAATCTTGAAGCGTTGCAGTTTTACACGCAAAAACTGTCGGCGCAATGCACCGCACTTTCCGATGCCTTAACCCGACAACAGCAAAACGACCAACCCTTTCCGCGCAAAACC‑AAGGAAGAACCTAAACCCGCCGGTAAACGCCGTCATCCCGTACACAGTTTGCCGCCCCGTGAACGGTTAGCGAAATATTATGAc                                                                                                                                                     >  1:617325/1‑227 (MQ=255)                                                                                                                                ggCGGATCCCAGCAATCTTGAAGCGTTGCAGTTTTACACGCAAAAACTGTCGGCGCAATGCACCGCACTTTCCGATGCCTTAACCCGACAACAGCAAAACGACCAACCCTTTCCGCGCAAAACC‑AAGGAAGAACCTAAACCCGCCGGTAAACGCCGTCATCCCGTACACAGTTTGCCGCCCCGTGAACGGTTAGCGAAATATTATGAc                                                                                                                                                     >  1:264901/1‑208 (MQ=255)                                                                                                                                    gATCCCAGCAATCTTGAAGCGTTGCAGTTTTACACGCAAAAACTGTCGGCGCAATGCACCGCACTTTCCGATGCCTTAACCCGACAACAGCAAAACGACCAACCCTTTCCGCGCAAAACC‑AAGGAAGAACCTAAACCCGCCGGTAAACGCCGTCATCCCGTACACAGTTTGCCGCCCCGTGAACGGTTAGCGAAATATTATGACTATCTGGCATCGTTCAATGAAAAAATTCAGGTTGAACAGGATACGtt                                                                                                      >  1:191763/1‑251 (MQ=255)                                                                                                                                         cAGCAATCTTGAAGCGTTGCAGTTTTACACGCAAAAACTGTCGGCGCAATGCACCGCACTTTCCGATGCCTTAACCCGACAACAGCAAAACGACCAACCCTTTCCGCGCAAAACC‑AAGGAAGAACCTAAACCCGCCGGTAAACGCCGTCATCCCGTACACAGTTTGCCGCCCCGTGAACGGTTAGCGAAATATTATGACTATCTGGCAt                                                                                                                                           >  1:283233/1‑209 (MQ=255)                                                                                                                                              aTCTTGAAGCGTTGCAGTTTTACACGCAAAAACTGTCGGCGCAATGCACCGCACTTTCCGATGCCTTAACCCGACAACAGCAAAACGACCAACCCTTTCCGCGCAAAACC‑AAGGAAGAACCTAAACCCGCCGGTAAACGCCGTCATCCCGTACACAGTTTGCCGCCCCGTGAACGGTTAGCGAAATATTATGACTATCTGGCATCGTTCAATGAAAAAATTCAGGTTGAACAGGATACGTTGGAAAAAGca                                                                                            >  1:566494/1‑251 (MQ=255)                                                                                                                                              aTCTTGAAGCGTTGCAGTTTTACACGCAAAAACTGTCGGCGCAATGCACCGCACTTTCCGATGCCTTAACCCGACAACAGCAAAACGACCAACCCTTTCCGCGCAAAACC‑AAGGAAGAACCTAAACCCGCCGGTAAACGCCGTCATCCCGTACACAGTTTGCCGCCCCGTGAACGGTTAGCGAAATATTATGACTATCTGGCATCGTTCAATGAAAAAATTCAGGTTGAACAGGATACGTTGGAAAAAGca                                                                                            >  1:223314/1‑251 (MQ=255)                                                                                                                                                cTTGAAGCGTTGCAGTTTTACACGCAAAAACTGTCGGCGCAATGCACCGCACTTTCCGATGCCTTAACCCGACAACAGCAAAACGACCAACCCTTTCCGCGCAAAACC‑AAGGAAGAACCTAAACCCGCCGGTAAACGCCGTCATCCCGTACACAGTTTGCCGCCCCGTGCACGGTTAGCGAAATATTATGACTATCTGGCATCGTTCAATGAAAAAATTCAGGTTGAACAGGATACGtt                                                                                                      >  1:431994/1‑239 (MQ=255)                                                                                                                                                            cAGTTTTACACGCAAAAACTGTCGGCGCAATGCACCGCACTTTCCGATGCCTTAACCCGACAACAGCAAAACGACCAACCCTTTCCGCGCAAAACC‑AAGGAAGAACCTAAACCCGCCGGTAAACGCCGTCATCCCGTACACAGTTTGCCGCCCCGTGAACGGTTAGCGAAATATTATGACTATCTGGCATCGTTCAATGAAAAAATTCAGGTTGAACAGGATACGTTGGAAAAAGCACAGCGTGAAGGTCg                                                                              >  1:238722/1‑251 (MQ=255)                                                                                                                                                              gTTTTACACGCAAAAACTGTCGGCGCAATGCACCGCACTTTCCGATGCCTTAACCCGACAACAGCAAAACGACCAACCCTTTCCGCGCAAAACC‑AAGGAAGAACCTAAACCCGCCGGTAAACGCCGTCATCCCGTACACAGTTTGCCGCCCCGTGAACGGTTAGCGAAATATTATGACTATCTGGCATCGTTCAATGAAAAAATTc                                                                                                                         >  1:613221/1‑206 (MQ=255)                                                                                                                                                              gTTTTACACGCAAAAACTGTCGGCGCAATGCACCGCACTTTCCGATGCCTTAACCCGACAACAGCAAAACGACCAACCCTTTCCGCGCAAAACC‑AAGGAAGAACCTAAACCCGCCGGTAAACGCCGTCATCCCGTACACAGTTTGCCGCCCCGTGAACGGTTAGCGAAATATTATGACTATCTGGCATCGTTCAATGAAAAAATTCAGGTTGAACAGGATACGTTGGAAAAAGCACAGCGTGAAGGTCGa                                                                             >  1:715709/1‑250 (MQ=255)                                                                                                                                                                          aaaccggccgccccaaccccccGCACTTCCCGATCCCTAACCCCAACAACAGCAAACCCACCACCCCCTCCCGCAAAAAACA‑AAGAAAGACCCAAACCCCGCCGGTAAACGCCGTCATCCCGTACACAGTTTGCCGCCCCGTGAACGGTTAGCGAAATATTATGACTATCTGGCATCGTTCAATGAAAAAATTCAGGTTGAACAGGATACGTTGGAAAAAGCACAGCGTGAAGGTCGACTTGTTAATAAAc                                                                <  1:16127/231‑1 (MQ=255)                                                                                                                                                                              cTGTCGGCGCAATGCACCGCACTTTCCGATGCCTTAACCCGACAACAGCAAAACGACCAACCCTTTCCGCGCAAAACC‑AAGGAAGAACCTAAACCCGCCGGTAAACGCCGTCATCCCGTACACAGTTTGCCGCCCCGTGAACGGTTAGCGAAATATTATGACTATCTGGCATCGTTCAATGAAAAAATTCAGGTTGAACAGGATACGTTGGAAAAAGCACAGCGTGAAGGTCGACTTGTTAATAAACAAAt                                                            >  1:630748/1‑251 (MQ=255)                                                                                                                                                                                     cgcAATGCACCGCACTTTCCGATGCCTTAACCCGACAACAGCAAAACGACCAACCCTTTCCGCGCAAAACC‑AAGGAAGAACCTAAACCCGCCGGTAAACGCCGTCATCCCGTACACAGTTTGCCGCCCCGTGAACGGTTAGCGAAATATTATGACTATCTGGCATCGTTc                                                                                                                                      >  1:679062/1‑170 (MQ=255)                                                                                                                                                                                                     ttCCGATGCCTTAACCCGACAACAGCAAAACGACCAACCCTTTCCGCGCAAAACC‑AAGGAAGAACCTAAACCCGCCGGTAAACGCCGTCATCCCGTACACAGTTTGCCGCCCCGTGAACGGTTAGCGAAATATTATGACTATCTGGCATCGTTCAATGAAAAAATTCAGGTTGAACAGGATACGTTGGAAAAAGCACAGCGTGAAGGTCGACTTGTTAATAAACAAATGCTGGAGCAATTAGAACAgcgc                                      >  1:840360/1‑250 (MQ=255)                                                                                                                                                                                                            gCCTTAACCCGACAACAGCAAAACGACCAACCCTTTCCGCGCAAAACC‑AAGGAAGAACCTAAACCCGCCGGTAAACGCCGTCATCCCGTACACAGTTTGCCGCCCCGTGAACGGTTAGCGAAATATTATGACTATCTGGCATCGTTCAATGAAAAAATTCAGGTTGAACAGGATACGTTGGAAAAAGCACAGCGTGAAGGTCGACTTGTTAATAAACAAATGCTGGAGCAATTAGAAc                                           >  1:123989/1‑238 (MQ=255)                                                                                                                                                                                                                   cccGACAACAGCAAAACGACCAACCCTTTCCGCGCAAAACC‑AAGGAAGAACCTAAACCCGCCGGTAAACGCCGTCATCCCGTACACAGTTTGCCGCCCCGTGAACGGTTAGCGAAATATTATGACTATCTGGCATCGTTCAATGAAAAAATTCAGGTTGAACAGGATACGTTGGAAAAAGCACAGCGTGAAGGTCGACTTGTtaat                                                                    >  1:673311/1‑206 (MQ=255)                                                                                                                                                                                                                          acaGCAAAACGACCAACCCTTTCCGCGCAAAACC‑AAGGAAGAACCTAAACCCGCCGGTAAACGCCGTCATCCCGTACACAGTTTGCCGCCCCGTGAACGGTTAGCGAAATATTATGACTATCTGGCATCGTTCAATGAAAAAATTCAGGTTGAACAGGATACGTTGGAAAAAGCACAGCGTGAAGGTCGACTTGTTAATAAACAAATGCTGGAGCAATTAGAAc                                           >  1:512050/1‑224 (MQ=255)                                                                                                                                                                                                                           caGCAAAACGACCAACCCTTTCCGCGCAAAACC‑AAGGAAGAACCTAAACCCGCCGGTAAACGCCGTCATCCCGTACACAGTTTGCCGCCCCGTGAACGGTTAGCGAAATATTATGACTATCTGGCATCGTTCAATGAAAAAATTCAGGTTGAACAGGATACGTTGGAAAAAGCACAGCGTGAAGGTCGACTTGTTAATAAACAAATGCTGGAGCAATTAGAACAGCGCCGTGCCCGTTGTTTGGAAGCGAt               >  1:27393/1‑251 (MQ=255)                                                                                                                                                                                                                                        aaCCCTTTCCGCGCAAAACC‑AAGGAAGAACCTAAACCCGCCGGTAAACGCCGTCATCCCGTACACAGTTTGCCGCCCCGTGAACGGTTAGCGAAATATTATGACTATCTGGCATCGTTCAATGAAAAAATTCAGGTTGAACAGGATACGTTGGAAAAAGCACAGCGTGAAGGTCGACTTGTTAATAAACAAATGCTGGAGCAATTAGAACAGCGCCGTGCCCGTTGTTTGGAAGCGATTGACGTGCTGgaa  >  1:593926/1‑251 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                 CGCGTTATGACGCGCAAAAGGAAGGAACGGTGTATGCCAAATTTTCGGCAAATTTATTTACGGAAAACCGGCAAAAAGTAGCATTTTATTTTCAGCAAATCGAACAAACCTTTGCGCGTCTTGAGCAGGCGGATCCCAGCAATCTTGAAGCGTTGCAGTTTTACACGCAAAAACTGTCGGCGCAATGCACCGCACTTTCCGATGCCTTAACCCGACAACAGCAAAACGACCAACCCTTTCCGAGCAAAACC‑AAGGAAGAACCTAAACCCGCCGGTAAACGCCGTCATCCCGTACACAGTTTGCCGCCCCGTGAACGGTTAGCGAAATATTATGACTATCTGGCATCGTTCAATGAAAAAATTCAGGTTGAACAGGATACGTTGGAAAAAGCACAGCGTGAAGGTCGACTTGTTAATAAACAAATGCTGGAGCAATTAGAACAGCGCCGTGCCCGTTGTTTGGAAGCGATTGACGTGCTGGAA  >  1/2021162‑2021643

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑