Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	2,019,116	A→G	H143R (CAC→CGC)	unknown →	Ribonuclease T

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	2,019,116	0	A	G	100.0%	225.8 / NA	78	H143R (CAC→CGC)	unknown	Ribonuclease T
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (49/29);  total (49/29)

CATTTTCACATTCAAGCTTTTGAAGGGGCGAATATCGATCCCGAATCCCTTAAATTTAACGGCATTGATATTGATAACCCGTTGCGTGGTGCGGTGAGCGAAAATATCGCCATCAGCGAAATGTTCAAGATGGTACGTAAAGCGCAAAAAGATGCGGATTGCCAGCGATCCATTATCGTGGCGCATAATGCGGCGTTCGATCAGGGGTTTCTCATGGCGGCGGCACAACGTATGAATGCTAAACACAACCCATTCCACCCCTTTTCCACCTTTGACACGGCAACCTTGAGCGGTTTTATGTTCGGGCAAACCGTGTTGGTGAAAGCCTGCCAAGCAGCAAAAATCCCTTTTGACAGCCAGCAGGCACATTCAGCGTTATATGACACGGAACGCACTGCCGAATTGTTTTGTTATATGGTGAATCATTTGAAGCAATTGGGTGGGTTTCCGCATCTTGTAGCGAATGATTAAGAACATCGAAAATTT  >  1/2018872‑2019357                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                  cATTTTCACATTCAAGCTTTTGAAGGGGCGAATATCGATCCCGAATCCCTTAAATTTAACGGCATTGATATTGATAACCCGTTGCGTGGTGCGGTGAGCGAAAATATCGCCATCAGCGAAATGTTCAAGATGGTACGTAAAGCGCAAAAAGATGCGGATTGCCAGCGATCCATTATCGTGGCGCATAATGCGGCGTTCGATCAGGGGTTTCTCATGGCGGCGGCACAACGTATGAATGCTAAACGCAAccc                                                                                                                                                                                                                                             <  1:85349/251‑1 (MQ=255)     ttCACATTCAAGCTTTTGAAGGGGCGAATATCGATCCCGAATCCCTTAAATTTAACGGCATTGATATTGATAACCCGTTGCGTGGTGCGGTGAGCGAAAATATCGCCATCAGCGAAATGTTCAAGATGGTACGTAAAGCGCAAAAAGATGCGGATTGCCAGCGATCCATTATCGTGGCGCATAATGCGGCGTTCGATCAGGGGTTTCTCATGGCGGCGGCACAACGTATGAATGCTAAACGCAACCCAtt                                                                                                                                                                                                                                          >  1:28091/1‑250 (MQ=255)      tCACATTCAAGCTTTTGAAGGGGCGAATATCGATCCCGAATCCCTTAAATTTAACGGCATTGATATTGATAACCCGTTGCGTGGTGCGGTGAGCGAAAATATCGCCATCAGCGAAATGTTCAAGATGGTACGTAAAGCGCAAAAAGATGCGGATTGCCAGCGATCCATTATCGTGGCGCATAATGCGGCGTTCGATCAGGGGTTTCTCATGGCGGCGGCACAACGTATGAATGCTAAACGCAACCCATTcc                                                                                                                                                                                                                                        <  1:313252/251‑1 (MQ=255)                 cTTTTGAAGGGGCGAATATCGATCCCGAATCCCTTAAATTTAACGGCATTGATATTGATAACCCGTTGCGTGGTGCGGTGAGCGAAAATATCGCCATCAGCGAAATGTTCAAGATGGTACGTAAAGCGCAAAAAGATGCGGATTGCCAGCGATCCATTATCGTGGCGCATAATGCGGCGTTCGATCAGGGGTTTCTCATGGCGGCGGCACAACGTATGAATGCTAAACGCAACCCATTCCACCCCTTTTc                                                                                                                                                                                                                              >  1:487265/1‑250 (MQ=255)                           ggCGAATATCGATCCCGAATCCCTTAAATTTAACGGCATTGATATTGATAACCCGTTGCGTGGTGCGGTGAGCGAAAATATCGCCATCAGCGAAATGTTCAAGATGGTACGTAAAGCGCAAAAAGATGCGGATTGCCAGCGATCCATTATCGTGGCGCATAATGCGGCGTTCGATCAGGGGTTTCTCATGGCGGCGGCACAACGTATGAATGCTAAACGCAACCCATTCCACCCCTTTTCCACCTTTGaca                                                                                                                                                                                                                   >  1:789505/1‑251 (MQ=255)                                  atCGATCCCGAATCCCTTAAATTTAACGGCATTGATATTGATAACCCGTTGCGTGGTGCGGTGAGCGAAAATATCGCCATCAGCGAAATGTTCAAGATGGTACGTAAAGCGCAAAAAGATGCGGATTGCCAGCGATCCATTATCGTGGCGCATAATGCGGCGTTCGATCAGGGGTTTCTCATGGCGGCGGCACAACGTATGAATGCTAAACGCAACCCATTCCACCCCTTTTCCACCTTTGACACGGCaa                                                                                                                                                                                                             >  1:888206/1‑250 (MQ=255)                                       tCCCGAATCCCTTAAATTTAACGGCATTGATATTGATAACCCGTTGCGTGGTGCGGTGAGCGAAAATATCGCCATCAGCGAAATGTTCAAGATGGTACGTAAAGCGCAAAAAGATGCGGATTGCCAGCGATCCATTATCGTGGCGCATAATGCGGCGTTCGATCAGGGGTTTCTCATGGCGGCGGCACAACGTATGAATGCTAAACGCAACCCATTCCACCCCTTTTCCACCTTTGACACGGCAACCTTGa                                                                                                                                                                                                       >  1:727045/1‑251 (MQ=255)                                               cccTTAAATTTAACGGCATTGATATTGATAACCCGTTGCGTGGTGCGGTGAGCGAAAATATCGCCATCAGCGAAATGTTCAAGATGGTACGTAAAGCGCAAAAAGATGCGGATTGCCAGCGATCCATTATCGTGGCGCATAATGCGGCGTTCGATCAGGGGTTTCTCATGGCGGCGGCACAACGTATGAATGCTAAACGCAACCCATTCCACCCCTTTTCCACCTTTGACACGGCAACCTTGAGCGGtttt                                                                                                                                                                                               >  1:27027/1‑251 (MQ=255)                                                              gCATTGATATTGATAACCCGTTGCGTGGTGCGGTGAGCGAAAATATCGCCATCAGCGAAATGTTCAAGATGGTACGTAAAGCGCAAAAAGATGCGGATTGCCAGCGATCCATTATCGTGGCGCATAATGCGGCGTTCGATCAGGGGTTTCTCATGGCGGCGGCACAACGTATGAATGCTAAACGCAACCCATTCCACCCCTTTTCCACCTTTGACACGGCAACCt                                                                                                                                                                                                          <  1:494150/225‑1 (MQ=255)                                                              gCATTGATATTGATAACCCGTTGCGTGGTGCGGTGAGCGAAAATATCGCCATCAGCGAAATGTTCAAGATGGTACGTAAAGCGCAAAAAGATGCGGATTGCCAGCGATCCATTATCGTGGCGCATAATGCGGCGTTCGATCAGGGGTTTCTCATGGCGGCGGCACAACGTATGAATGCTAAACGCAACCCATTCCACCCCTTTTCCACCTTTGACACGGCAACCTTGAGCGGTTTTATGTTCGGGCAAAc                                                                                                                                                                                 >  1:330215/1‑250 (MQ=255)                                                              gCATTGATATTGATAACCCGTTGCGTGGTGCGGTGAGCGAAAATATCGCCATCAGCGAAATGTTCAAGATGGTACGTAAAGCGCAAAAAGATGCGGATTGCCAGCGATCCATTATCGTGGCGCATAATGCGGCGTTCGATCAGGGGTTTCTCATGGCGGCGGCACAACGTATGAATGCTAAACGCAACCCATTCCACCCCTTTTCCACCTTTGACACGGCAACCTTGAGCGGTTTTATGTTCGGGCAAAc                                                                                                                                                                                 >  1:112990/1‑250 (MQ=255)                                                                   gatattgataACCCGTTGCGTGGTGCGGTGAGCGAAAATATCGCCATCAGCGAAATGTTCAAGATGGTACGTAAAGCGCAAAAAGATGCGGATTGCCAGCGATCCATTATCGTGGCGCATAATGCGGCGTTCGATCAGGGGTTTCTCATGGCGGCGGCACAACGTATGAATGCTAAACGCAACCCATTCCACCCCTTTTCCACCTTTGACACGGCAACCt                                                                                                                                                                                                          >  1:305570/1‑220 (MQ=255)                                                                   gatattgataACCCGTTGCGTGGTGCGGTGAGCGAAAATATCGCCATCAGCGAAATGTTCAAGATGGTACGTAAAGCGCAAAAAGATGCGGATTGCCAGCGATCCATTATCGTGGCGCATAATGCGGCGTTCGATCAGGGGTTTCTCATGGCGGCGGCACAACGTATGAATGCTAAACGCAACCCATTCCACCCCTTTTCCACCTTTGACACGGCAACCTTGAGCGGTTTTATGt                                                                                                                                                                                           >  1:139167/1‑235 (MQ=255)                                                                   gatattgataACCCGTTGCGTGGTGCGGTGAGCGAAAATATCGCCATCAGCGAAATGTTCAAGATGGTACGTAAAGCGCAAAAAGATGCGGATTGCCAGCGATCCATTATCGTGGCGCATAATGCGGCGTTCGATCAGGGGTTTCTCATGGCGGCGGCACAACGTATGAATGCTAAACGCAACCCATTCCACCCCTTTTCCACCTTTGACACGGCAACCTTGAGCGGTTTTATGTTCGGGCAAACCg                                                                                                                                                                               >  1:525027/1‑247 (MQ=255)                                                                   gatattgataACCCGTTGCGTGGTGCGGTGAGCGAAAATATCGCCATCAGCGAAATGTTCAAGATGGTACGTAAAGCGCAAAAAGATGCGGATTGCCAGCGATCCATTATCGTGGCGCATAATGCGGCGTTCGATCAGGGGTTTCTCATGGCGGCGGCACAACGTATGAATGCTAAACGCAACCCATTCCACCCCTTTTCCACCTTTGACACGGCAACCTTGAGCGGTTTTATGTTCGGGCAAACCGTGtt                                                                                                                                                                           >  1:786213/1‑251 (MQ=255)                                                                   gatattgataACCCGTTGCGTGGTGCGGTGAGCGAAAATATCGCCATCAGCGAAATGTTCAAGATGGTACGTAAAGCGCAAAAAGATGCGGATTGCCAGCGATCCATTATCGTGGCGCATAATGCGGCGTTCGATCAGGGGTTTCTCATGGCGGCGGCACAACGTATGAATGCTAAACGCAACCCATTCCACCCCTTTTCCACCTTTGACACGGCAACCTTGAGCGGTTTTATGTTCGGGCAAACCGTGtt                                                                                                                                                                           >  1:814342/1‑251 (MQ=255)                                                                     tattgataACCCGTTGCGTGGTGCGGTGAGCGAAAATATCGCCATCAGCGAAATGTTCAAGATGGTACGTAAAGCGCAAAAAGATGCGGATTGCCAGCGATCCATTATCGTGGCGCATAATGCGGCGTTCGATCAGGGGTTTCTCATGGCGGCGGCACAACGTATGAATGCTAAACGCAACCCATTCCACCCCTTTTCCACCTTTGACACGGCAACCTTGAGCGGTTTTATGTTCGGGCAAACCGTGTTgg                                                                                                                                                                         <  1:93521/251‑1 (MQ=255)                                                                        tgataACCCGTTGCGTGGTGCGGTGAGCGAAAATATCGCCATCAGCGAAATGTTCAAGATGGTACGTAAAGCGCAAAAAGATGCGGATTGCCAGCGATCCATTATCGTGGCGCATAATGCGGCGTTCGATCAGGGGTTTCTCATGGCGGCGGCACAACGTATGAATGCTAAACGCAACCCATTCCACCCCTTTTCCACCTTTGac                                                                                                                                                                                                                    >  1:192529/1‑205 (MQ=255)                                                                         gataACCCGTTGCGTGGTGCGGTGAGCGAAAATATCGCCATCAGCGAAATGTTCAAGATGGTACGTAAAGCGCAAAAAGATGCGGATTGCCAGCGATCCATTATCGTGGCGCATAATGCGGCGTTCGATCAGGGGTTTCTCATGGCGGCGGCACAACGTATGAATGCTAAACGCAACCCATTCCACCCCTTTTCCACCTTTGACACGGCAACCTTGAGCGGTTTTATGTTCGGGCAAACCGTGTTGGTGaa                                                                                                                                                                     >  1:837769/1‑251 (MQ=255)                                                                             aCCCGTTGCGTGGTGCGGTGAGCGAAAATATCGCCATCAGCGAAATGTTCAAGATGGTACGTAAAGCGCAAAAAGATGCGGATTGCCAGCGATCCATTATCGTGGCGCATAATGCGGCGTTCGATCAGGGGTTTCTCATGGCGGCGGCACAACGTATGAATGCTAAACGCAACCCATTCCACCCCTTTTCCACCTTTGACACGGCAACCTTGAGCGGTTTTATGTTCGGGCAAACCGTGTTGGTg                                                                                                                                                                       >  1:729437/1‑245 (MQ=255)                                                                             aCCCGTTGCGTGGTGCGGTGAGCGAAAATATCGCCATCAGCGAAATGTTCAAGATGGTACGTAAAGCGCAAAAAGATGCGGATTGCCAGCGATCCATTATCGTGGCGCATAATGCGGCGTTCGATCAGGGGTTTCTCATGGCGGCGGCACAACGTATGAATGCTAAACGCAACCCATTCCACCCCTTTTCCACCTTTGACACGGCAACCTTGAGCGGTTTTATGTTCGGGCAAACCGTGTTGGTGAAAGcc                                                                                                                                                                 <  1:725584/251‑1 (MQ=255)                                                                               ccGTTGCGTGGTGCGGTGAGCGAAAATATCGCCATCAGCGAAATGTTCAAGATGGTACGTAAAGCGCAAAAAGATGCGGATTGCCAGCGATCCATTATCGTGGCGCATAATGCGGCGTTCGATCAGGGGTTTCTCATGGCGGCGGCACAACGTATGAATGCTAAACGCAACCCATTCCACCCCTTTTCCACCTTTGACACGGCAACCTTGAGCGGTTTTATGTTCGGGCAAACCGTGTTGGTGAAAGc                                                                                                                                                                  >  1:55987/1‑248 (MQ=255)                                                                                 gTTGCGTGGTGCGGTGAGCGAAAATATCGCCATCAGCGAAATGTTCAAGATGGTACGTAAAGCGCAAAAAGATGCGGATTGCCAGCGATCCATTATCGTGGCGCATAATGCGGCGTTCGATCAGGGGTTTCTCATGGCGGCGGCACAACGTATGAATGCTAAACGCAACCCATTCCACCCCTTTTCCAc                                                                                                                                                                                                                           >  1:301793/1‑189 (MQ=255)                                                                                      gtggtgCGGTGAGCGAAAATATCGCCATCAGCGAAATGTTCAAGATGGTACGTAAAGCGCAAAAAGATGCGGATTGCCAGCGATCCATTATCGTGGCGCATAATGCGGCGTTCGATCAGGGGTTTCTCATGGCGGCGGCACAACGTATGAATGCTAAACGCAACCCATTCCACCCCTTTTCCAc                                                                                                                                                                                                                           <  1:397223/184‑1 (MQ=255)                                                                                      gtggtgCGGTGAGCGAAAATATCGCCATCAGCGAAATGTTCAAGATGGTACGTAAAGCGCAAAAAGATGCGGATTGCCAGCGATCCATTATCGTGGCGCATAATGCGGCGTTCGATCAGGGGTTTCTCATGGCGGCGGCACAACGTATGAATGCTAAACGCAACCCATTCCACCCCTTTTCCACCTTTGACACGGCAACCTTGAGCGGTTTTATGTTCGGGCAAACCGTGTTg                                                                                                                                                                          <  1:183871/233‑1 (MQ=255)                                                                                                 aGCGAAAATATCGCCATCAGCGAAATGTTCAAGATGGTACGTAAAGCGCAAAAAGATGCGGATTGCCAGCGATCCATTATCGTGGCGCATAATGCGGCGTTCGATCAGGGGTTTCTCATGGCGGCGGCACAACGTATGAATGCTAAACGCAACCCATTCCACCCCTTTTCCACCTTTGACACGGCAACCTTGAGCGGTTTTATGTTCGGGCAAACCGTGTTGGTGAAAGCCTGCCAAGCAGCAAAAATcc                                                                                                                                              >  1:198029/1‑250 (MQ=255)                                                                                                        atatCGCCATCAGCGAAATGTTCAAGATGGTACGTAAAGCGCAAAAAGATGCGGATTGCCAGCGATCCATTATCGTGGCGCATAATGCGGCGTTCGATCAGGGGTTTCTCATGGCGGCGGCACAACGTATGAATGCTAAACGCAACCCATTCCACCCCTTTTCCACCTTTGACACGGCAACCTTGAGCGGTTTTATGTTCGGGCAAACCGTGTTGGTGAAAGCCTGCCAAGCAGCAAAAATCCCTTTTGAc                                                                                                                                      <  1:584102/251‑1 (MQ=255)                                                                                                                         aTGTTCAAGATGGTACGTAAAGCGCAAAAAGATGCGGATTGCCAGCGATCCATTATCGTGGCGCATAATGCGGCGTTCGATCAGGGGTTTCTCATGGCGGCGGCACAACGTATGAATGCTAAACGCAACCCATTCCACCCCTTTTCCACCTTTGACACGGCAACCTTGAGCGGTTTTATGTTCGGGCAAACCGTGTTGGTGAAAGCCTGCCAAGCAGCAAAAATCCCTTTTGACAGCCAGCAGGCACAtt                                                                                                                      >  1:485753/1‑250 (MQ=255)                                                                                                                         aTGTTCAAGATGGTACGTAAAGCGCAAAAAGATGCGGATTGCCAGCGATCCATTATCGTGGCGCATAATGCGGCGTTCGATCAGGGGTTTCTCATGGCGGCGGCACAACGTATGAATGCTAAACGCAACCCATTCCACCCCTTTTCCACCTTTGACACGGCAACCTTGAGCGGTTTTATGTTCGGGCAAACCGTGTTGGTGAAAGCCTGCCAAGCAGCAAAAATCCCTTTTGACAGCCAGCAGGCACATTc                                                                                                                     <  1:878466/251‑1 (MQ=255)                                                                                                                           gTTCAAGGTGGTACGTAAAGCGCAAAAAGATGCGGATTGCCAGCGATCCATTATCGTGGCGCATAATGCGGCGTTCGATCAGGGGTTTCTCATGGCGGCGGCACAACGTATGAATGCTAAACGCAACCCATTCCACCCCTTTTCCACCTTTGACACGGCAACCTTGAGCGGTTTTATGTTCGGGCAAACCGTGTTGGTGAAAGCCTGCCAAGCAGCAAAAATCCCTTTTGACAGCCAGCAGGCACATTCAg                                                                                                                   >  1:598377/1‑251 (MQ=255)                                                                                                                           gTTCAAGATGGTACGTAAAGCGCAAAAAGATGCGGATTGCCAGCGATCCATTATCGTGGCGCATAATGCGGCGTTCGATCAGGGGTTTCTCATGGCGGCGGCACAACGTATGAATGCTAAACGCAACCCATTCCACCCCTTTTCCACCTTTGACACGGCAACCTTGAGCGGTTTTATGTTCGGGCAAACCGTGTTGGTGAAAGCCTGCCAAGCAGCAAAAATCCCTTTTGACAGCCAGCAGGCACATTCAg                                                                                                                   >  1:608724/1‑251 (MQ=255)                                                                                                                           gTTCAAGATGGTACGTAAAGCGCAAAAAGATGCGGATTGCCAGCGATCCATTATCGTGGCGCATAATGCGGCGTTCGATCAGGGGTTTCTCATGGCGGCGGCACAACGTATGAATGCTAAACGCAACCCATTCCACCCCTTTTCCACCTTTGACACGGCAACCTTGAGCAGTTTTATGTTCGGGCAAACCGTGTTGGTGAAAGCCTGCCAAGCAGCAAAAATCCCTTTTGACAGCCAGCAGGCACATTCAg                                                                                                                   >  1:441999/1‑251 (MQ=255)                                                                                                                              cAAGATGGTACGTAAAGCGCAAAAAGATGCGGATTGCCAGCGATCCATTATCGTGGCGCATAATGCGGCGTTCGATCAGGGGTTTCTCATGGCGGCGGCACAACGTATGAATGCTAAACGCAACCCATTCCACCCCTTTTCCACCTTTGACACGGCAACCTTGAGCGGTTTTATGTTCGGGCAAACCGTGTTGGTGAAAGCCTGCCAAGCAGCAAAAATCCCTTTTGACAGCCAGCAGGc                                                                                                                           >  1:271386/1‑240 (MQ=255)                                                                                                                              cAAGATGGTACGTAAAGCGCAAAAAGATGCGGATTGCCAGCGATCCATTATCGTGGCGCATAATGCGGCGTTCGATCAGGGGTTTCTCATGGCGGCGGCACAACGTATGAATGCTAAACGCAACCCATTCCACCCCTTTTCCACCTTTGACACGGCAACCTTGAGCGGTTTTATGTTCGGGCAAACCGTGTTGGTGAAAGCCTGCCAAGCAGCAAAAATCCCTTTTGACAGCCAGCAGGCACATTCAGCGt                                                                                                                >  1:14387/1‑251 (MQ=255)                                                                                                                                aGATGGTACGTAAAGCGCAAAAAGATGCGGATTGCCAGCGATCCATTATCGTGGCGCATAATGCGGCGTTCGATCAGGGGTTTCTCATGGCGGCGGCACAACGTATGAATGCTAAACGCAACCCATTCCACCCCTTTTCCACCTTTGACACGGCAACCTTGAGCGGTTTTATGTTCGGGCAAACCGTGTTGGTGAAAGCCTGCCAAGCAGCAAAAATCCCTTTTGACAGCCAGCAGGCACATTCAGCGTta                                                                                                              >  1:199611/1‑251 (MQ=255)                                                                                                                                  aTGGTACGTAAAGCGCAAAAAGATGCGGATTGCCAGCGATCCATTATCGTGGCGCATAATGCGGCGTTCGATCAGGGGTTTCTCATGGCGGCGGCACAACGTATGAATGCTAAACGCAACCCATTCCACCCCTTTTCCACCTTTGACACGGCAACCTTGAGCGGTTTTATGTTCGGGCAAACCGTGTTGGTGAAAg                                                                                                                                                                   <  1:340058/196‑1 (MQ=255)                                                                                                                                  aTGGTACGTAAAGCGCAAAAAGATGCGGATTGCCAGCGATCCATTATCGTGGCGCATAATGCGGCGTTCGATCAGGGGTTTCTCATGGCGGCGGCACAACGTATGAATGCTAAACGCAACCCATTCCACCCCTTTTCCACCTTTGACACGGCAACCTTGAGCGGTTTTATGTTCGGGCAAACCGTGTTGGTGAAAGCCTGCCAAGCAGCAAAAATCCCTTTTGACAGCCAGCAGGCACATTCAGCGTtata                                                                                                            >  1:150495/1‑251 (MQ=255)                                                                                                                                    ggTACGTAAAGCGCAAAAAGATGCGGATTGCCAGCGATCCATTATCGTGGCGCATAATGCGGCGTTCGATCAGGGGTTTCTCATGGCGGCGGCACAACGTATGAATGCTAAACGCAACCCATTCCACCCCTTTTCCACCTTTGACACGGCAACCTTGAGCGGTTTTATGTTCGGGCAAACCGTGTTGGTGAAAGCCTGCCAAGCAGCAAAAATCCCTTTTGACAGCCAGCAGGCACATTCAGCGTTATATg                                                                                                          >  1:73366/1‑251 (MQ=255)                                                                                                                                    ggTACGTAAAGCGCAAAAAGATGCGGATTGCCAGCGATCCATTATCGTGGCGCATAATGCGGCGTTCGATCAGGGGTTTCTCATGGCGGCGGCACAACGTATGAATGCTAAACGCAACCCATTCCACCCCTTTTCCACCTTTGACACGGCAACCTTGAGCGGTTTTATGTTCGGGCAAACCGTGTTGGTGAAAGCCTGCCAAGCAGCAAAAATCCCTTTTGACAGCCAGCAGGCACATTCAGCGTTATATg                                                                                                          >  1:194027/1‑251 (MQ=255)                                                                                                                                    ggTACGTAAAGCGCAAAAAGATGCGGATTGCCAGCGATCCATTATCGTGGCGCATAATGCGGCGTTCGATCAGGGGTTTCTCATGGCGGCGGCACAACGTATGAATGCTAAACGCAACCCATTCCACCCCTTTTCCACCTTTGACACGGCAACCTTGAGCGGTTTTATGTTCGGGCAAACCGTGTTGGTGAAAGCCTGCCAAGCAGCAAAAATCCCTTTTGACAGCCAGCAGGCACATTCAGCGTTATATg                                                                                                          >  1:851200/1‑251 (MQ=255)                                                                                                                                    ggTACGTAAAGCGCAAAAAGATGCGGATTGCCAGCGATCCATTATCGTGGCGCATAATGCGGCGTTCGATCAGGGGTTTCTCATGGCGGCGGCACAACGTATGAATGCTAAACGCAACCCATTCCACCCCTTTTCCACCTTTGACACGGCAACCTTGAGCGGTTTTATGTTCGGGCAAACCGTGTTGGTGAAAGCCTGCCAAGCAGCAAAAATCCCTTTTGACAGCCAGCAGGCACATTCAGCGTTATATg                                                                                                          >  1:87610/1‑251 (MQ=255)                                                                                                                                      tACGTAAAGCGCAAAAAGATGCGGATTGCCAGCGATCCATTATCGTGGCGCATAATGCGGCGTTCGATCAGGGGTTTCTCATGGCGGCGGCACAACGTATGAATGCTAAACGCAACCCATTCCACCCCTTTTCCACCTTTGACACGGCAACCTTGAGCGGTTTTATGTTCGGGCAAACCGTGTTGGTGAAAGCCTGCCAAGCAGCAAAAATCCCTTTTGACAGCCAGCAGGCACATTCAGCGTTATATGac                                                                                                        <  1:390007/251‑1 (MQ=255)                                                                                                                                      tACGTAAAGCGCAAAAAGATGCGGATTGCCAGCGATCCATTATCGTGGCGCATAATGCGGCGTTCGATCAGGGGTTTCTCATGGCGGCGGCACAACGTATGAATGCTAAACGCAACCCATTCCACCCCTTTTCCACCTTTGACACGGCAACCTTGAGCGGTTTTATGTTCGGGCAAACCGTGTTGGTGAAAGCCTGCCAAGCAGCAAAAATCCCTTTTGACAGCCAGCAGGCACATTCAGCGTTATATGa                                                                                                         <  1:899094/250‑1 (MQ=255)                                                                                                                                        cGTAAAGCGCAAAAAGATGCGGATTGCCAGCGATCCATTATCGTGGCGCATAATGCGGCGTTCGATCAGGGGTTTCTCATGGCGGCGGCACAACGTATGAATGCTAAACGCAACCCATTCCACCCCTTTTCCACCTTTGACACGGCAACCTTGAGCGGTTTTATGTTCGGGCAAACCGTGTTGGTGAAAGCCTGCCAAGCAGCAAAAATCCCTTTTGACAGCCAGCGGGCACATTCAGCGTTATATGacac                                                                                                      <  1:36759/251‑1 (MQ=255)                                                                                                                                         gTAAAGCGCAAAAAGATGCGGATTGCCAGCGATCCATTATCGTGGCGCATAATGCGGCGTTCGATCAGGGGTTTCTCATGGCGGCGGCACAACGTATGAATGCTAAACGCAACCCATTCCACCCCTTTTCCACCTTTGACACGGCAACCTTGAGCGGTTTTATGTTCGGGCAAACCGTGTTGGTGAAAGCCTGCCAAGCAGCAAAAATCCCTTTTGACAGCCAGCAGGCACATTCAGCGTTATATGACACg                                                                                                     >  1:479085/1‑251 (MQ=255)                                                                                                                                              gcgcAAAAAGATGCGGATTGCCAGCGATCCATTATCGTGGCGCATAATGCGGCGTTCGATCAGGGGTTTCTCATGGCGGCGGCACAACGTATGAATGCTAAACGCAACCCATTCCACCCCTTTTCCACCTTTGACACGGCAACCTTGAGCGGTTTTATGTTCGGGCAAACCGTGTTGGTGAAAGCCTGCCAAGCAGCAAAAATCCCTTTTGACAGCCAGCAGGCACATTCAGCGTTatat                                                                                                           >  1:865904/1‑240 (MQ=255)                                                                                                                                                 cAAAAAGATGCGGATTGCCAGCGATCCATTATCGTGGCGCATAATGCGGCGTTCGATCAGGGGTTTCTCATGGCGGCGGCACAACGTATGAATGCTAAACGCAACCCATTCCACCCCTTTTCCACCTTTGACACGGCAACCTTGAGCGGTTTTATGTTCGGGCAAACCGTGTTGGTGAAAGCCTGCCAAGCAGCAAAAATCCCTTTTGACAGCCAGCAGGCACATTCAGCGTTATATGACACGGAACGCAc                                                                                             <  1:83200/251‑1 (MQ=255)                                                                                                                                                                     gCGATCCATTATCGTGGCGCATAATGCGGCGTTCGATCAGGGGTTTCTCATGGCGGCGGCACAACGTATGAATGCTAAACGCAACCCATTCCACCCCTTTTCCACCTTTGACACGGCAACCTTGAGCGGTTTTATGTTCGGGCAAACCGTGTTGGTGAAAGCCTGCCAAGCAGCAAAAATCCCTTTTGACAGCCAGCAGGCACATTCAGCg                                                                                                                 >  1:535875/1‑211 (MQ=255)                                                                                                                                                                      cGATCCATTATCGTGGCGCATAATGCGGCGTTCGATCAGGGGTTTCTCATGGCGGCGGCACAACGTATGAATGCTAAACGCAACCCATTCCACCCCTTTTCCACCTTTGACACGGCAACCTTGAGCGGTTTTATGTTCGGGCAAACCGTGTTGGTGAAAGCCTGCCAAGCAGCAAAAATCCCTTTTGACAGCCAGCAGGCACATTCAGCGTTATATGACACGGAACGCACTGCCGAATTGTTTTGTta                                                                           <  1:608083/248‑1 (MQ=255)                                                                                                                                                                        aTCCATTATCGTGGCGCATAATGCGGCGTTCGATCAGGGGTTTCTCATGGCGGCGGCACAACGTATGAATGCTAAACGCAACCCATTCCACCCCTTTTCCACCTTTGACACGGCAACCTTGAGCGGTTTTATGTTCGGGCAAACCGTGTTGGTGAAAGCCTGCCAAGCAGCAAAAATCCCTTTTGACAGCCAGCAGGCACATTCAGCGTTATATGACACGGAACGCACTGCCGAATTGTTTTGTTATATgg                                                                      >  1:261704/1‑251 (MQ=255)                                                                                                                                                                        aTCCATTATCGTGGCGCATAATGCGGCGTTCGATCAGGGGTTTCTCATGGCGGCGGCACAACGTATGAATGCTAAACGCAACCCATTCCACCCCTTTTCCACCTTTGACACGGCAACCTTGAGCGGTTTTATGTTCGGGCAAACCGTGTTGGTGAAAGCCTGCCAAGCAGCAAAAATCCCTTTTGACAGCCAGCAGGCACATTCAGCGTTATATGACACGGAACGCACTGCCGAATTGTTTTGTTATATgg                                                                      <  1:306842/251‑1 (MQ=255)                                                                                                                                                                          ccATTATCGTGGCGCATAATGCGGCGTTCGATCAGGGGTTTCTCATGGCGGCGGCACAACGTATGAATGCTAAACGCAACCCATTCCACCCCTTTTCCACCTTTGACACGGCAACCTTGAGCGGTTTTATGtt                                                                                                                                                                                          <  1:220637/133‑1 (MQ=255)                                                                                                                                                                             ttATCGTGGCGCATAATGCGGCGTTCGATCAGGGGTTTCTCATGGCGGCGGCACAACGTATGAATGCTAAACGCAACCCATTCCACCCCTTTTCCACCTTTGACACGGCAACCTTGAGCGGTTTTATGTTCGGGCAAACCGTGTTGGTGAAAGCCTGCCAAGCAGCAAAAATCCCTTTTGACAGCCAGCAGGCACATTCAGCGTTATATGACACGGAACGCACTGCCGAATTGTTTTGTTATATGGTGAAt                                                                 >  1:87719/1‑251 (MQ=255)                                                                                                                                                                               aTCGTGGCGCATAATGCGGCGTTCGATCAGGGGTTTCTCATGGCGGCGGCACAACGTATGAATGCTAAACGCAACCCATTCCACCCCTTTTCCACCTTTGACACGGCAACCTTGAGCGGTTTTATGTTCGGGCAAACCGTGTTGGTGAAAGCCTGCCAAGCAGCAAAAATCCCTTTTGACAGCCAGCAgg                                                                                                                            >  1:151332/1‑190 (MQ=255)                                                                                                                                                                               aTCGTGGCGCATAATGCGGCGTTCGATCAGGGGTTTCTCATGGCGGCGGCACAACGTATGAATGCTAAACGCAACCCATTCCACCCCTTTTCCACCTTTGACACGGCAACCTTGAGCGGTTTTATGTTCGGGCAAACCGTGTTGGTGAAAGCCTGCCAAGCAGCAAAAATCCCTTTTGACAGCCAGCAGGCACATTCAGCGTTATATGACACGGAACGCACTGCCGAATTGTTTTGTTATATGGTGAATCa                                                               >  1:373908/1‑251 (MQ=255)                                                                                                                                                                                  gTGGCGCATAATGCGGCGTTCGATCAGGGGTTTCTCATGGCGGCGGCACAACGTATGAATGCTAAACGCAACCCATTCCACCCCTTTTCCACCTTTGACACGGCAACCTTGAGCAGTTTTATGTTCGGGCAAACCGTGTTGGTGAAAGcc                                                                                                                                                                 <  1:626033/150‑1 (MQ=255)                                                                                                                                                                                       gcATAATGCGGCGTTCGATCAGGGGTTTCTCATGGCGGCGGCACAACGTATGAATGCTAAACGCAACCCATTCCACCCCTTTTCCACCTTTGACACGGCAACCTTGAGCGGTTTTATGTTCGGGCAAACCGTGTTGGTGAAAGCCTGCCAAGCAGCAAAAATCCCTTTTGACAGCCAGCAGGCACATTCAGCGTTATATGACACGGAACGCACTGCCGAATTGTTTTGTTATATGGTGAATCATTTGAAg                                                        >  1:185937/1‑250 (MQ=255)                                                                                                                                                                                         aTAATGCGGCGTTCGATCAGGGGTTTCTCATGGCGGCGGCACAACGTATGAATGCTAAACGCAACCCATTCCACCCCTTTTCCACCTTTGACACGGCAACCTTGAGCGGTTTTATGTTCGGGCAAACCGTGTTGGTGAAAGCCTGCCAAGCAGCAAAAATCCCTTTTGACAGCCAGCAGGCACATTCAGCGTTATATGac                                                                                                        <  1:634978/200‑1 (MQ=255)                                                                                                                                                                                            aTGCGGCGTTCGATCAGGGGTTTCTCATGGCGGCGGCACAACGTATGAATGCTAAACGCAACCCATTCCACCCCTTTTCCACCTTTGACACGGCAACCTTGAGCGGTTTTATGTTCGGGCAAACCGTGTTGGTGAAAGCCTGCCAAGCAGCAAAAATCCCTTTTGACAGCCAGCAGGCACATTCAGCGTTATATGACACGGAACGCACTGCCGAATTGTTTTGTTATATgg                                                                      <  1:549116/231‑1 (MQ=255)                                                                                                                                                                                                 gcgTTCGATCAGGGGTTTCTCATGGCGGCGGCACAACGTATGAATGCTAAACGCAACCCATTCCACCCCTTTTCCACCTTTGACACGGCAACCTTGAGCGGTTTTATGTTCGGGCAAACCGTGTTGGTGAAAGCCTGCCAAGCAGCAAAAATCCCTTTTGACAGCCAGCCGGCACATTCAGCGTTATATGACACGGAACGCACTGCCGAATTGTTTTGTTATATGGTGAATCATTTGAAGCAATtgggtgg                                             >  1:434460/1‑251 (MQ=255)                                                                                                                                                                                                  cgTTCGATCAGGGGTTTCTCATGGCGGCGGCACAACGTATGAATGCTAAACGCAACCCATTCCACCCCTTTTCCACCTTTGACACGGCAACCTTGAGCGGTTTTATGTTCGGGCAAACCGTGTTGGTGAAAGCCTGCCAAGCAGCAAAAATCCCTTTTGACAGCCAGCAGGCACATTCAGCGTTATATGACACGGAACGCACTGCCGAATTGTTTTGTTATATGGTGAATCATTTGAAGCAATtgggtggg                                            <  1:286946/251‑1 (MQ=255)                                                                                                                                                                                                    ttCGATCAGGGGTTTCTCATGGCGGCGGCACAACGTATGAATGCTAAACGCAAccc                                                                                                                                                                                                                                             <  1:84213/56‑1 (MQ=255)                                                                                                                                                                                                                   ctcATGGCGGCGGCACAACGTATGAATGCTAAACGCAACCCATTCCACCCCTTTTCCACCTTTGACACGGCAACCTTGAGCGGTTTTATGTTCGGGCAAACCGTGTTGGTGAAAGcc                                                                                                                                                                 >  1:483810/1‑117 (MQ=255)                                                                                                                                                                                                                   ctcATGGCGGCGGCACAACGTATGAATGCTAAACGCAACCCATTCCACCCCTTTTCCACCTTTGACACGGCAACCTTGAGCGGTTTTATGTTCGGGCAAACCGTGTTGGTGAAAGCCTGCCAAGCAGCAAAAATCCCTTTTGACAGCCAGCAGGCACATTCAGCGTTATATGACACGGAACGCACTGCCGAATTGTTTTGTTATATGGTGAATCATTTGAAGCAATTGGGTGGGTTTCCGCATCTTGTAGc                           <  1:847107/251‑1 (MQ=255)                                                                                                                                                                                                                       tGGCGGCGGCACAACGTATGAATGCTAAACGCAACCCATTCCACCCCTTTTCCACCTTTGACACGGCAACCTTGAGCGGTTTTATGTTCGGGCAAACCGTGTTGGTGAAAGCCTGCCAAGCAGCAAAAATCCCTTTTGACAGCCAGCAGGCACATTCAGCGTTATATGACACGGAACGCACTGCCGAATTGTTTTGTTATATGGTGAATCATTTGAAGCAATTGGGTGGGTTTCCGCATCTTGTAGCGAAt                       <  1:774356/251‑1 (MQ=255)                                                                                                                                                                                                                          cggcggcACAACGTATGAATGCTAAACGCAACCCATTCCACCCCTTTTCCACCTTTGACACGGCAACCTTGAGCGGTTTTATGTTCGGGCAAACCGTGTTGGTGAAAGCCTGCCAAGCAGCAAAAATCCCTTTTGACAGCCAGCAGGCACATTCAGCGTTATATGACACGGAACGCACTGCCGAATTGTTTTGTTATATGGTGAATCATTTGAAGCAATTGGGTGGGTTTCCGCATCTTGTAGCGAATGAt                    <  1:282665/251‑1 (MQ=255)                                                                                                                                                                                                                              ggcACAACGTATGAATGCTAAACGCAACCCATTCCACCCCTTTTCCACCTTTGACACGGCAACCTTGAGCGGTTTTATGTTCGGGCAAACCGTGTTGGTGAAAGCCTGCCAAGCAGCAAAAATCCCTTTTGACAGCCAGCAGGCACATTCAGCGTTATATGACACGGAAc                                                                                                 <  1:685551/170‑1 (MQ=255)                                                                                                                                                                                                                                 acaACGTATGAATGCTAAACGCAACCCATTCCACCCCTTTTCCACCTTTGACACGGCAACCTTGAGCGGTTTTATGTTCGGGCAAACCGTGTTGGTGAAAGCCTGCCAAGCAGCAAAAATCCCTTTTGACAGCCAGCAGGCACATTCAGCGTTATATGACACGGAACGCACTGCCGAATTGTTTTGTTATATGGTGAATCATTTGAAGCAATTGGGTGGGTTTCCGCATCTTGTAGCGAATGATTAAGAAc             <  1:491775/251‑1 (MQ=255)                                                                                                                                                                                                                                    aCGTATGAATGCTAAACGCAATCCATTCCACCCCTTTTCCACCTTTGACACGGCAACCTTGAGCGGTTTTATGTTCGGGCAAACCGTGTTGGTGAAAGCCTGCCAAGCAGCAAAAATCCCTTTTGACAGCCAGCAGGCACATTCAGCGTTATATGACACGGAACGCACTGCCGAATTGTTTTGTTATATgg                                                                      <  1:432502/191‑1 (MQ=255)                                                                                                                                                                                                                                    aCGTATGAATGCTAAACGCAACCCATTCCACCCCTTTTCCACCTTTGACACGGCAACCTTGAGCGGTTTTATGTTCGGGCAAACCGTGTTGGTGAAAGCCTGCCAAGCAGCAAAAATCCCt                                                                                                                                            >  1:511060/1‑121 (MQ=255)                                                                                                                                                                                                                                    aCGTATGAATGCTAAACGCAACCCATTCCACCCCTTTTCCACCTTTGACACGGCAACCTTGAGCGGTTTTATGTTCGGGCAAACCGTGTTGGTGAAAGCCTGCCAAGCAGCAAAAATCCCTTTTGACAGCCAGCAGGCACATTCAGCGTTATATGACACGGAACGCACTGCCGAATTGTTTTGTTATATGGTGAATCATTTGAAGCAATTGGGTGGGTTTCCGCATCTTGTAGCGAATGATTAAGAACAt           >  1:8417/1‑250 (MQ=255)                                                                                                                                                                                                                                    aCGTATGAATGCTAAACGCAACCCATTCCACCCCTTTTCCACCTTTGACACGGCAACCTTGAGCGGTTTTATGTTCGGGCAAACCGTGTTGGTGAAAGCCTGCCAAGCAGCAAAAATCCCTTTTGACAGCCAGCAGGCACATTCAGCGTTATATGACACGGAACGCACTGCCGAATTGTTTTGTTATATGGTGAATCATTTGAAGCAATTGGGTGGGTTTCCGCATCTTGTAGCGAATGATTAAGAACAt           >  1:710401/1‑250 (MQ=255)                                                                                                                                                                                                                                    aCGTATGAATGCTAAACGCAACCCATTCCACCCCTTTTCCACCTTTGACACGGCAACCTTGAGCGGTTTTATGTTCGGGCAAACCGTGTTGGTGAAAGCCTGCCAAGCAGCAAAAATCCCTTTTGACAGCCAGCAGGCACATTCAGCGTTATATGACACGGAACGCACTGCCGAATTGTTTTGTTATATGGTGAATCATTTGAAGCAATTGGGTGGGTTTCCGCATCTTGTAGCGAATGATTAAGAACAt           >  1:118489/1‑250 (MQ=255)                                                                                                                                                                                                                                      gTATGAATGCTAAACGCAACCCATTCCACCCCTTTTCCACCTTTGACACGGCAACCTTGAGCGGTTTTATGTTCGGGCAAACCGTGTTGGTGAAAGCCTGCCAAGCAGCAAAAATCCCTTTTGACAGCCAGCAGGCACAt                                                                                                                       >  1:726753/1‑140 (MQ=255)                                                                                                                                                                                                                                      gTATGAATGCTAAACGCAACCCATTCCACCCCTTTTCCACCTTTGACACGGCAACCTTGAGCGGTTTTATGTTCGGGCAAACCGTGTTGGTGAAAGCCTGCCAAGCAGCAAAAATCCCTTTTGACAGCCAGCAGGCACATTCAGCGTTATATGACACGGAACGCACTGCCGAATTGTTTTGTTATATGGTGAATCATTTGAAGCAATTGGGTGGGTTTCCGCATCTTGTAGCGAATGATTAAGAACATCGa        >  1:756893/1‑251 (MQ=255)                                                                                                                                                                                                                                      gTATGAATGCTAAACGCAACCCATTCCACCCCTTTTCCACCTTTGACACGGCAACCTTGAGCGGTTTTATGTTCGGGCAAACCGTGTTGGTGAAAGCCTGCCAAGCAGCAAAAATCCCTTTTGACAGCCAGCAGGCACATTCAGCGTTATATGACACGGAACGCACTGCCGAATTGTTTTGTTATATGGTGAATCATTTGAAGCAATTGGGTGGGTTTCCGCATCTTGTAGCGAATGATTAAGAACATCGa        >  1:681250/1‑251 (MQ=255)                                                                                                                                                                                                                                      gTATGAATGCTAAACGCAACCCATTCCACCCCTTTTCCACCTTTGACACGGCAACCTTGAGCGGTTTTATGTTCGGGCAAACCGTGTTGGTGAAAGCCTGCCAAGCAGCAAAAATCCCTTTTGACAGCCAGCAGGCACATTCAGCGTTATATGACACGGAACGCACTGCCGAATTGTTTTGTTATATGGTGAATCATTTGAAGCAATTGGGTGGGTTTCCGCATCTTGTAGCGAATGATTAAGAACATCGa        >  1:112515/1‑251 (MQ=255)                                                                                                                                                                                                                                            aTGCTAAACGCAACCCATTCCACCCCTTTTCCACCTTTGACACGGCAACCTTGAGCGGTTTTATGTTCGGGCAAACCGTGTTGGTGAAAGCCTGCCAAGCAGCAAAAATCCCTTTTGACAGCCAGCAGGCACATTCAGCGTTATATGACACGGAACGCACTGCCGAATTGTTTTGTTATATGGTGAATCATTTGAAGCAATTGGGTGGGTTTCCGCATCTTGTAGCGAATGATTAAGAACATCGAAAAttt  <  1:824667/251‑1 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                  CATTTTCACATTCAAGCTTTTGAAGGGGCGAATATCGATCCCGAATCCCTTAAATTTAACGGCATTGATATTGATAACCCGTTGCGTGGTGCGGTGAGCGAAAATATCGCCATCAGCGAAATGTTCAAGATGGTACGTAAAGCGCAAAAAGATGCGGATTGCCAGCGATCCATTATCGTGGCGCATAATGCGGCGTTCGATCAGGGGTTTCTCATGGCGGCGGCACAACGTATGAATGCTAAACACAACCCATTCCACCCCTTTTCCACCTTTGACACGGCAACCTTGAGCGGTTTTATGTTCGGGCAAACCGTGTTGGTGAAAGCCTGCCAAGCAGCAAAAATCCCTTTTGACAGCCAGCAGGCACATTCAGCGTTATATGACACGGAACGCACTGCCGAATTGTTTTGTTATATGGTGAATCATTTGAAGCAATTGGGTGGGTTTCCGCATCTTGTAGCGAATGATTAAGAACATCGAAAATTT  >  1/2018872‑2019357

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑