Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	2,018,525	A→G	T111A (ACC→GCC)	unknown →	Lactoylglutathione lyase (EC 4.4.1.5)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	2,018,525	0	A	G	100.0%	220.2 / NA	73	T111A (ACC→GCC)	unknown	Lactoylglutathione lyase (EC 4.4.1.5)
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (30/43);  total (30/43)

CGAAAATCCCGAATACAAATATTCTCTGGCATTTTTAGGTTACGACGATGAAGACAAAACCTCCGTGTTGGAACTGACTTACAACTGGGGCGTGGATAAATACGAACTCGGCACCGCTTACGGGCATATCGCCATCGGCACGGACGATATTTACGCCACCTGCGAAGCGGTGCGCAAAGCCGGTGGCAATGTCACCCGTGAGCCCGGCCCGGTAAAAGGCGGCAAAACAGTGATTACCTTTGTGGAAGATCCGGACGGTTATAAAATCGAATTTATCGAAAACAAAAACGCACAAGCCGGATTAGGCAACTAGTTCGCACCAAAAGTGCGGTAGAAAATTCAAACGTTTTTTAACGTGGATTATGAACGATTATCCACGTTTTATTTTTAGGATGAACTATGTCAGAAACCCAAGAAACCATTAATTACAATATATTAAAACATCGATTCCGAGGTTATTTGCCGGTGGTAATC  >  1/2018290‑2018763                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                               cGAAAATCCCGAATACAAATATTCTCTGGCATTTTTAGGTTACGACGATGAAGACAAAACCTCCGTGTTGGAACTGACTTACAACTGGGGCGTGGATAAATACGAACTCGGCACCGCTTACGGGCATATCGCCATCGGCACGGACGATATTTACGCCACCTGCGAAGCGGTGCGCAAAGCCGGTGGCAATGTCACCCGTGAGCCCGGCCCGGTAAAAGGCGGCAAAACAGTGATTGCCTTTGTGGAAGATc                                                                                                                                                                                                                                 <  1:662039/251‑1 (MQ=255)     aaTCCCGAATACAAATATTCTCTGGCATTTTTAGGTTACGACGATGAAGACAAAACCTCCGTGTTGGAACTGACTTACAACTGGGGCGTGGATAAATACGAACTCGGCACCGCTTACGGGCATATCGCCATCGGCACGGACGATATTTACGCCACCTGCGAAGCGGTGCGCAAAGCCGGTGGCAATGTCACCCGTGAGCCCGGCCCGGTAAAAGGCGGCAAAACAGTGATTGCCTTTGTGGAAGATCCGGa                                                                                                                                                                                                                             <  1:104720/251‑1 (MQ=255)      aTCCCGAATACAAATATTCTCTGGCATTTTTAGGTTACGACGATGAAGACAAAACCTCCGTGTTGGAACTGACTTACAACTGGGGCGTGGATAAATACGAACTCGGCACCGCTTACGGGCATATCGCCATCGGCACGGACGATATTTACGCCACCTGCGAAGCGGTGCGCAAAGCCGGTGGCAATGTCACCCGTGAGCCCGGCCCGGTAAAAGGCGGCAAAACAGTGATTGCCTTTGTGGAAGATCCGGAc                                                                                                                                                                                                                            <  1:881085/251‑1 (MQ=255)       tCCCGAATACAAATATTCTCTGGCATTTTTAGGTTACGACGATGAAGACAAAACCTCCGTGTTGGAACTGACTTACAACTGGGGCGTGGATAAATACGAACTCGGCACCGCTTACGGGCATATCGCCATCGGCACGGACGATATTTACGCCACCTGCGAAGCGGTGCGCAAAGCCGGTGGCAATGTCACCCGTGAGCCCGGCCCGGTAAAAGGCGGCAAAACAGTGATTGCCTTTGTGGAAGATCCGGACg                                                                                                                                                                                                                           <  1:703175/251‑1 (MQ=255)            aaTACAAATATTCTCTGGCATTTTTAGGTTACGACGATGAAGACAAAACCTCCGTGTTGGAACTGACTTACAACTGGGGCGTGGATAAATACGAACTCGGCACCGCTTACGGGCATATCGCCATCGGCACGGACGATATTTACGCCACCTGCGAAGCGGTGCGCAAAGCCGGTGGCAATGTCACCCGTGAGCCCGGCCCGGTAAAAGGCGGCAAAACAGTGATTGCCTTTGTGGAAGATCCGGACGGTtat                                                                                                                                                                                                                      <  1:91300/251‑1 (MQ=255)             aTACAAATATTCTCTGGCATTTTTAGGTTACGACGATGAAGACAAAACCTCCGTGTTGGAACTGACTTACAACTGGGGCGTGGATAAATACGAACTCGGCACCGCTTACGGGCATATCGCCATCGGCACGGACGATATTTACGCCACCTGCGAAGCGGTGCGCAAAGCCGGTGGCAATGTCACCCGTGAGCCCGGCCCGGTAAAAGGCGGCAAAACAGTGATTGCCTTTGGGGAAGATCCGGACGGTtata                                                                                                                                                                                                                     >  1:276411/1‑251 (MQ=255)                cAAATATTCTCTGGCATTTTTAGGTTACGACGATGAAGACAAAACCTCCGTGTTGGAACTGACTTACAACTGGGGCGTGGATAAATACGAACTCGGCACCGCTTACGGGCATATCGCCATCGGCACGGACGATATTTACGCCACCTGCGAAGCGGTGCGCAAAGCCGGTGGCAATGTCACCCGTGAGCCCGGCCCGGTAAAAGGCGGCAAAACAGTGATTGCCTTTGTGGAAGATCCGGACGGTTATaaaa                                                                                                                                                                                                                  <  1:693554/251‑1 (MQ=255)                cAAATATTCTCTGGCATTTTTAGGTTACGACGATGAAGACAAAACCTCCGTGTTGGAACTGACTTACAACTGGGGCGTGGATAAATACGAACTCGGCACCGCTTACGGGCATATCGCCATCGGCACGGACGATATTTACGCCACCTGCGAAGCGGTGCGCAAAGCCGGTGGCAATGTCACCCGTGAGCCCGGCCCGGTAAAAGGCGGCAAAACAGTGATTGCCTTTGTGGAAGATCCGGACGGTTATaaaa                                                                                                                                                                                                                  <  1:634552/251‑1 (MQ=255)                cAAATATTCTCTGGCATTTTTAGGTTACGACGATGAAGACAAAACCTCCGTGTTGGAACTGACTTACAACTGGGGCGTGGATAAATACGAACTCGGCACCGCTTACGGGCATATCGCCATCGGCACGGACGATATTTACGCCACCTGCGAAGCGGTGCGCAAAGCCGGTGGCAATGTCACCCGTGAGCCCGGCCCGGTAAAAGGCGGCAAAACAGTGATTGCCTTTGTGGAAGATCCGGACGGTTATaaaa                                                                                                                                                                                                                  >  1:242927/1‑251 (MQ=255)                cAAATATTCTCTGGCATTTTTAGGTTACGACGATGAAGACAAAACCTCCGTGTTGGAACTGACTTACAACTGGGGCGTGGATAAATACGAACTCGGCACCGCTTACGGGCATATCGCCATCGGCACGGACGATATTTACGCCACCTGCGAAGCGGTGCGCAAAGCCGGTGGCAATGTCACCCGTGAGCCCGGCCCGGTAAAAGGCGGCAAAACAGTGATTGCCTTTGTGGAAGATCCGGACGGTTATaaaa                                                                                                                                                                                                                  >  1:498826/1‑251 (MQ=255)                                      ggTTACGACGATGAAGACAAAACCTCCGTGTTGGAACTGACTTACAACTGGGGCGTGGATAAATACGAACTCGGCACCGCTTACGGGCATATCGCCATCGGCACGGACGATATTTACGCCACCTGCGAAGCGGTGCGCAAAGCCGGTGGCAATGTCACCCGTGAGCCCGGCCCGGTAAAAGGCGGCAAAACAGTGATTGCCTTTGTGGAAGATCCGGACGGTTATAAAATCGAATTTATCGAAAACaaaaa                                                                                                                                                                                            >  1:817248/1‑251 (MQ=255)                                       gTTACGACGATGAAGACAAAACCTCCGTGTTGGAACTGACTTACAACTGGGGCGTGGATAAATACGAACTCGGCACCGCTTACGGGCATATCGCCATCGGCACGGACGATATTTACGCCACCTGCGAAGCGGTGCGCAAAGCCGGTGGCAATGTCACCCGTGAGCCCGGCCCGGTAAAAGGCGGCAAAACAGTGATTGCCTTTGTGGAAGATCCGGACGGTTATAAAATCGAATTTATCGAAAACaaaaa                                                                                                                                                                                            <  1:137120/250‑1 (MQ=255)                                               gaTGAAGACAAAACCTCCGTGTTGGAACTGACTTACAACTGGGGCGTGGATAAATACGAACTCGGCACCGCTTACGGGCATATCGCCATCGGCACGGACGATATTTACGCCACCTGCGAAGCGGTGCGCAAAGCCGGTGGCAATGTCACCCGTGAGCCCGGCCCGGTAAAAGGCGGCAAAACAGTGATTGCCTTTGTGGa                                                                                                                                                                                                                                      >  1:695873/1‑200 (MQ=255)                                                    aGACAAAACCTCCGTGTTGGAACTGACTTACAACTGGGGCGTGGATAAATACGAACTCGGCACCGCTTACGGGCATATCGCCATCGGCACGGACGATATTTACGCCACCTGCGAAGCGGTGCGCAAAGCCGGTGGCAATGTCACCCGTGAGCCCGGCCCGGTAAAAGGCGGCAAAACAGTGATTGCCTTTGTGGAAGATCCGGACGGTTATAAAATCGAATTTATCg                                                                                                                                                                                                      >  1:518946/1‑227 (MQ=255)                                                     gACAAAACCTCCGTGTTGGAACTGACTTACAACTGGGGCGTGGATAAATACGAACTCGGCACCGCTTACGGGCATATCGCCATCGGCACGGACGATATTTACGCCACCTGCGAAGCGGTGCGCAAAGCCGGTGGCAATGTCACCCGTGAGCCCGGCCCGGTAAAAGGCGGCAAAACAGTGATTGCCTTTGTGGAAGATCCGGACGGTTATAAAATCGAATTTATCGAAAACAAAAACGCACAAGCCGGAtt                                                                                                                                                                             >  1:530072/1‑251 (MQ=255)                                                       cAAAACCTCCGTGTTGGAACTGACTTACAACTGGGGCGTGGATAAATACGAACTCGGCACCGCTTACGGGCATATCGCCATCGGCACGGACGATATTTACGCCACCTGCGAAGCGGTGCGCAAAGCCGGTGGCAATGTCACCCGTGAGCCCGGCCCGGTAAAAGGCGGCAAAACAGTGATTGCCTTTGTGGAAGATCCGGACGGTTATAAAATCGAATTTATCGAAAACAAAAACGCACAAGCCGGATTAg                                                                                                                                                                           >  1:661677/1‑251 (MQ=255)                                                         aaaCCTCCGTGTTGGAACTGACTTACAACTGGGGCGTGGATAAATACGAACTCGGCACCGCTTACGGGCATATCGCCATCGGCACGGACGATATTTACGCCACCTGCGAAGCGGTGCGCAAAGCCGGTGGCAATGTCACCCGTGAGCCCGGCCCGGTAAAAGGCGGCAAAACAGTGATTGCCTTTGTGGAAGATCCGGACGGTTATAAAATCGAATTTATCGAAAACAAAAACGCACAAGCCGGATTAgg                                                                                                                                                                          >  1:422593/1‑250 (MQ=255)                                                                 gtgtTGGAACTGACTTACAACTGGGGCGTGGATAAATACGAACTCGGCACCGCTTACGGGCATATCGCCATCGGCACGGACGATATTTACGCCACCTGCGAAGCGGTGCGCAAAGCCGGTGGCAATGTCACCCGTGAGCCCGGCCCGGTAAAAGGCGGCAAAACAGTGATTGCCTTTGTGGAAGATCCGGACGGTTATAAAATCGAATTTATCGAAAACAAAAACGCACAAGCCGGATTAGGCAACTAGtt                                                                                                                                                                 >  1:655292/1‑251 (MQ=255)                                                                  tgtTGGAACTGACTTACAACTGGGGCGTGGATAAATACGAACTCGGCACCGCTTACGGGCATATCGCCATCGGCACGGACGATATTTACGCCACCTGCGAAGCGGTGCGCAAAGCCGGTGGCAATGTCACCCGTGAGCCCGGCCCGGTAAAAGGCGGCAAAACAGTGATTGCCTTTGTGGAAGATCCGGACGGTTATAAAATCGAATTTATCGAAAACAAAAACGCACAAGCCGGATTAGGCAACTAGtt                                                                                                                                                                 <  1:198896/250‑1 (MQ=255)                                                                            gACTTACAACTGGGGCGTGGATAAATACGAACTCGGCACCGCTTACGGGCATATCGCCATCGGCACGGACGATATTTACGCCACCTGCGAAGCGGTGCGCAAAGCCGGTGGCAATGTCACCCGTGAGCCCGGCCCGGTAAAAGGCGGCAAAACAGTGATTGCCTTTGTGGAAGATCCGGACGGTTATAAAATCGAATTTATCGAAAACAAAAACGCACAAGCCGGAtt                                                                                                                                                                             >  1:590278/1‑228 (MQ=255)                                                                            gACTTACAACTGGGGCGTGGATAAATACGAACTCGGCACCGCTTACGGGCATATCGCCATCGGCACGGACGATATTTACGCCACCTGCGAAGCGGTGCGCAAAGCCGGTGGCAATGTCACCCGTGAGCCCGGCCCGGTAAAAGGCGGCAAAACAGTGATTGCCTTTGTGGAAGATCCGGACGGTTATAAAATCGAATTTATCGAAAACAAAAACGCACAAGCCGGATTAGGCAACTAg                                                                                                                                                                   >  1:689082/1‑238 (MQ=255)                                                                            gACTTACAACTGGGGCGTGGATAAATACGAACTCGGCACCGCTTACGGGCATATCGCCATCGGCACGGACGATATTTACGCCACCTGCGAAGCGGTGCGCAAAGCCGGTGGCAATGTCACCCGTGAGCCCGGCCCGGTAAAAGGCGGCAAAACAGTGATTGCCTTTGTGGAAGATCCGGACGGTTATAAAATCGAATTTATCGAAAACAAAAACGCACAAGCCGGATTAGGCAACTAGTTCGCACCAAAAg                                                                                                                                                      >  1:531489/1‑251 (MQ=255)                                                                             aCTTACAACTGGGGCGTGGATAAATACGAACTCGGCACCGCTTACGGGCATATCGCCATCGGCACGGACGATATTTACGCCACCTGCGAAGCGGTGCGCAAAGCCGGTGGCAATGTCACCCGTGAGCCCGGCCCGGTAAAAGGCGGCAAAACAGTGATTGCCTTTGTGGAAGATCCGGACGGTTATAAAATCGAATTTATCGAAAACAAAAACGCACAAGCCGGATTAGGCAACTAGTTCGCACCAAAAGt                                                                                                                                                     >  1:536244/1‑251 (MQ=255)                                                                                        gggCGTGGATAAATACGAACTCGGCACCGCTTACGGGCATATCGCCATCGGCACGGACGATATTTACGCCACCTGCGAAGCGGTGCGCAAAGCCGGTGGCAATGTCACCCGTGAGCCCGGCCCGGTAAAAGGCGGCAAAACAGTGATTGCCTTTGTGGAAGATCCGGACGGTTATAAAATCGAATTTATCGAAAACAAAAACGCACAAGCCGGATTAGGCAACTAGTTCGCACCAAAAGTGCGGTAGaaa                                                                                                                                           <  1:811645/250‑1 (MQ=255)                                                                                                    aTACGAACTCGGCACCGCTTACGGGCATATCGCCATCGGCACGGACGATATTTACGCCACCTGCGAAGCGGTGCGCAAAGCCGGTGGCAATGTCACCCGTGAGCCCGGCCCGGTAAAAGGCGGCAAAACAGTGATTGCCTTTGTGGAAGATCCGGACGGTTATAAAATCGAATTTATCGAAAACAAAAACGCACAAGCCGGATTAGGCAACTAGtt                                                                                                                                                                 >  1:134264/1‑216 (MQ=255)                                                                                                          aCTCGGCACCGCTTACGGGCATATCGCCATCGGCACGGACGATATTTACGCCACCTGCGAAGCGGTGCGCAAAGCCGGTGGCAATGTCACCCGTGAGCCCGGCCCGGTAAAAGGCGGCAAAACAGTGATTGCCTTTGTGGAAGATCCGGACGGTTATAAAATCGAATTTATCGAAAACAAAAACGCACAAGcc                                                                                                                                                                                  <  1:12962/193‑1 (MQ=255)                                                                                                                    gCTTACGGGCATATCGCCATCGGCACGGACGATATTTACGCCACCTGCGAAGCGGTGCGCAAAGCCGGTGGCAATGTCACCCGTGAGCCCGGCCCGGTAAAAGGCGGCAAAACAGTGATTGCCTTTGTGGAAGATCCGGACGGTTATAAAATCGAATTTATCGAAAACAAAAACGCACAAGCCGGATTAGGCAACTAGTTCGCACCAAAAGTGCGGTAGAAAATTCAAACGTTTTTTAACGTGGATTATGa                                                                                                              >  1:529409/1‑251 (MQ=255)                                                                                                                    gCTTACGGGCATATCGCCATCGGCACGGACGATATTTACGCCACCTGCGAAGCGGTGCGCAAAGCCGGTGGCAATGTCACCCGTGAGCCCGGCCCGGTAAAAGGCGGCAAAACAGTGATTGCCTTTGTGGAAGATCCGGACGGTTATAAAATCGAATTTATCGAAAACAAAAACGCACAAGCCGGATTAGGCAACTAGTTCGCACCAAAAGTGCGGTAGAAAATTCAAACGTTTTTTAACGTGGATTATGa                                                                                                              >  1:508786/1‑251 (MQ=255)                                                                                                                    gCTTACGGGCATATCGCCATCGGCACGGACGATATTTACGCCACCTGCGAAGCGGTGCGCAAAGCCGGTGGCAATGTCACCCGTGAGCCCGGCCCGGTAAAAGGCGGCAAAACAGTGATTGCCTTTGTGGAAGATCCGGACGGTTATAAAATCGAATTTATCGAAAACAAAAACGCACAAGCCGGATTAGGCAACTAGTTCGCACCAAAAGTGCGGTAGAAAATTCAAACGTTTTTTAACGTGGATTATGa                                                                                                              >  1:147994/1‑251 (MQ=255)                                                                                                                     cTTACGGGCATATCGCCATCGGCACGGACGATATTTACGCCACCTGCGAAGCGGTGCGCAAAGCCGGTGGCAATGTCACCCGTGAGCCCGGCCCGGTAAAAGGCGGCAAAACAGTGATTGCCTTTGTGGAAGATCCGGACGGTTATAAAATCGAATTTATCGAAAACAAAAACGCACAAGCCGGATTAGGCAACTAGTTCGCACCAAAAGTGCGGTAGAAAAt                                                                                                                                         >  1:410781/1‑223 (MQ=255)                                                                                                                      ttACGGGCATATCGCCATCGGCACGGACGATATTTACGCCACCTGCGAAGCGGTGCGCAAAGCCGGTGGCAATGTCACCCGTGAGCCCGGCCCGGTAAAAGGCGGCAAAACAGTGATTGCCTTTGTGGAAGATCCGGACGGTTATAAAATCGAATTTATCGAAAACAAAAACGCACAAGCCGGATTAGGCAACTAGTTCGCACCAAAAGTGCGGTAGAAAATTCAAACGTTTTTTAACGTGGATTATGAAc                                                                                                            <  1:594532/251‑1 (MQ=255)                                                                                                                                          gCACGGACGATATTTACGCCACCTGCGAAGCGGTGCGCAAAGCCGGTGGCAATGTCACCCGTGAGCCCGGCCCGGTAAAAGGCGGCAAAACAGTGATTGCCTTTGTGGAAGATCCGGACGGTTATAAAATCGAATTTATCGAAAACAAAAACGCACAAGCCGGATTAGGCAACTAg                                                                                                                                                                   >  1:376342/1‑176 (MQ=255)                                                                                                                                              ggACGATATTTACGCCACCTGCGAAGCGGTGCGCAAAGCCGGTGGCAATGTCACCCGTGAGCCCGGCCCGGTAAAAGGCGGCAAAACAGTGATTGCCTTTGTGGAAGATCCGGACGGTTATaaa                                                                                                                                                                                                                   >  1:760212/1‑124 (MQ=255)                                                                                                                                               gACGATATTTACGCCACCTGCGAAGCGGTGCGCAAAGCCGGTGGCAATGTCACCCGTGAGCCCGGCCCGGTAAAAGGCGGCAAAACAGTGATTGCCTTTGTGGAAGATCCGGACGGTTATAAAATCGAATTTATCGAAAACAAAAACGCACAAGCCGGATTAGGCAACTAGtt                                                                                                                                                                 <  1:778090/173‑1 (MQ=255)                                                                                                                                               gACGATATTTACGCCACCTGCGAAGCGGTGCGCAAAGCCGGTGGCAATGTCACCCGTGAGCCCGGCCCGGTAAAAGGCGGCAAAACAGTGATTGCCTTTGTGGAAGATCCGGACGGTTATAAAATCGAATTTATCGAAAACAAAAACGCACAAGCCGGATTAGGCAACTAGTTCGCACCAAAAGTGCGGTAGAAAATTCAAACGTTTTTTAACGTGGATTATGaa                                                                                                             >  1:275576/1‑225 (MQ=255)                                                                                                                                                    tatTTACGCCACCTGCGAAGCGGTGCGCAAAGCCGGTGGCAATGTCACCCGTGAGCCCGGCCCGGTAAAAGGCGGCAAAACAGTGATTGCCTTTGTGGAAGATCCGGACGGTTATAAAATCGAATTTATCGAAAACAAAAACGCACAAGCCGGATTAGGCAACTAGTTCGCACCAAAAGTGCGGTAGAAAATTCAAACGTTTTTTAACGTGGATTATGAACGATTATCCACGTTTTAtt                                                                                          <  1:319396/239‑1 (MQ=255)                                                                                                                                                          cGCCACCTGCGAAGCGGTGCGCAAAGCCGGTGGCAATGTCACCCGTGAGCCCGGCCCGGTAAAAGGCGGCAAAACAGTGATTGCCTTTGTGGAAGATCCGGACGGTTATAAAATCGAATTTATCGAAAACAAAAACGCACAAGCCGGATTAGGCAACTAGTTCGCACCAAAAGTGCGGTAGAAAATTCAAACGTTTTTTAACGTGGATTATGAACGATTATCCACGTTTTATTTTTAGGATGAACTATGTc                                                                        <  1:282938/251‑1 (MQ=255)                                                                                                                                                          cGCCACCTGCGAAGCGGTGCGCAAAGCCGGTGGCAATGTCACCCGTGAGCCCGGCCCGGTAAAAGGCGGCAAAACAGTGATTGCCTTTGTGGAAGATCCGGACGGTTATAAAATCGAATTTATCGAAAACAAAAACGCACAAGCCGGATTAGGCAACTAGTTCGCACCAAAAGTGCGGTAGAAAATTCAAACGTTTTTTAACGTGGATTATGAACGATTATCCACGTTTTATTTTTAGGATGAACTATGTc                                                                        <  1:471014/251‑1 (MQ=255)                                                                                                                                                          cGCCACCTGCGAAGCGGTGCGCAAAGCCGGTGGCAATGTCACCCGTGAGCCCGGCCCGGTAAAAGGCGGCAAAACAGTGATTGCCTTTGTGGAAGATCCGGACGGTTATAAAATCGAATTTATCGAAAACAAAAACGCACAAGCCGGATTAGGCAACTAGTTCGCACCAAAAGTGCGGTAGAAAATTCAAACGTTTTTTAACGTGGATTATGAACGATTATCCACGTTTTATTTTTAGGATGAACTATGTc                                                                        <  1:411428/251‑1 (MQ=255)                                                                                                                                                          cGCCACCTGCGAAGCGGTGCGCAAAGCCGGTGGCAATGTCACCCGTGAGCCCGGCCCGGTAAAAGGCGGCAAAACAGTGATTGCCTTTGTGGAAGATCCGGACGGTTATAAAATCGAATTTATCGAAAACAAAAACGCACAAGCCGGATTAGGCAACTAGTTCGCACCAAAAGTGCGGTAGAAAATGCAAACGTTTTTTAACGTGGATTATGAACGATTATCCACGTTTTATTTTTAGGATGAACTATGTc                                                                        <  1:681633/251‑1 (MQ=255)                                                                                                                                                            ccACCTGCGAAGCGGTGCGCAAAGCCGGTGGCAATGTCACCCGTGAGCCCGGCCCGGTAAAAGGCGGCAAAACAGTGATTGCCTTTGTGGAAGATCCGGACGGTTATAAAATCGAATTTATCGAAAACAAAAACGCACAAGCCGGATTAGGCAACTAGtt                                                                                                                                                                 >  1:823498/1‑160 (MQ=255)                                                                                                                                                            ccACCTGCGAAGCGGTGCGCAAAGCCGGTGGCAATGTCACCCGTGAGCCCGGCCCGGTAAAAGGCGGCAAAACAGTGATTGCCTTTGTGGAAGATCCGGACGGTTATAAAATCGAATTTATCGAAAACAAAAACGCACAAGCCGGATTAGGCAACTAGTTCGCACCAAAAGTGCGGTAGAAAATTCAAACGTTTTTTAACGTGGATTATGAACGATTATCCACGTTTTATTTTTAGGATGAACTATGTCAg                                                                      <  1:873731/251‑1 (MQ=255)                                                                                                                                                              aCCTGCGAAGCGGTGCGCAAAGCCGGTGGCAATGTCACCCGTGAGCCCGGCCCGGTAAAAGGCGGCAAAACAGTGATTGCCTTTGTGGAAGATCCGGACGGTTATAAAATCGAATTTATCGAAAACAAAAACGCACAAGCCGGATTAGGCAACTAGTTCGCACCAAAAGTGCGGTAGAAAATTCAAACGTTTTTTAACGTGGATTATGAACGATTATCCACGTTTTATTTTTAGGATGAACTATGTCAGaa                                                                    <  1:547386/251‑1 (MQ=255)                                                                                                                                                              aCCTGCGAAGCGGTGCGCAAAGCCGGTGGCAATGTCACCCGTGAGCCCGGCCCGGTAAAAGGCGGCAAAACAGTGATTGCCTTTGTGGAAGATCCGGACGGTTATAAAATCGAATTTATCGAAAACAAAAACGCACAAGCCGGATTAGGCAACTAGTTCGCACCAAAAGTGCGGTAGAAAATTCAAACGTTTTTTAACGTGGATTATGAACGATTATCCACGTTTTATTTTTAGGATGAACTATGTCAGaa                                                                    <  1:333737/251‑1 (MQ=255)                                                                                                                                                               ccTGCGAAGCGGTGCGCAAAGCCGGTGGCAATGTCACCCGTGAGCCCGGCCCGGTAAAAGGCGGCAAAACAGTGATTGCCTTTGTGGAAGATCCGGACGGTTATAAAATCGAATTTATCGAAAACAAAAACGCACAAGCCGGATTAGGCAACTAGTTCGCACCAAAAGTGCGGTAGAAAATTCAAACGTTTTTTAACGTGGAt                                                                                                                   <  1:310852/203‑1 (MQ=255)                                                                                                                                                               ccTGCGAAGCGGTGCGCAAAGCCGGTGGCAATGTCACCCGTGAGCCCGGCCCGGTAAAAGGCGGCAAAACAGTGATTGCCTTTGTGGAAGATCCGGACGGTTATAAAATCGAATTTATCGAAAACAAAAACGCACAAGCCGGATTAGGCAACTAGTTCGCACCAAAAGTGCGGTAGAAAATTCAAACGTTTTTTAACGTGGATTATGAACGATTATCCACGTTTTATTTTTAGGATGAACTATGTc                                                                        >  1:179280/1‑246 (MQ=255)                                                                                                                                                                cTGCGAAGCGGTGCGCAAAGCCGGTGGCAATGTCACCCGTGAGCCCGGCCCGGTAAAAGGCGGCAAAACAGTGATTGCCTTTGTGGAAGATCCGGACGGTTATAAAATCGAATTTATCGAAAACAAAAACGCACAAGCCGGATTAGGCAACTAGTTCGCACCAAAAGTGCGGTAGAAAATTCAAACGTTTTTTAACGTGGATTATGAACGATTATCCACGTTTTATTTTTAGGATGAACTATGTCAGAAAc                                                                  <  1:887468/251‑1 (MQ=255)                                                                                                                                                                 tGCGAAGCGGTGCGCAAAGCCGGTGGCAATGTCACCCGTGAGCCCGGCCCGGTAAAAGGCGGCAAAACAGTGATTGCCTTTGTGGAAGATCCGGACGGTTATAAAATCGAATTTATCGAAAACAAAAACGCACAAGCCGGATTAGGCAACTAGTTCGCACCAAAAGTGCGGTAGAAAATTCAAACGTTTTTTAACGTGGATTATGAACGATTATCCACGTTTTATTTTTAGGATGAACTATGTCAGAAAcc                                                                 <  1:392733/251‑1 (MQ=255)                                                                                                                                                                  gCGAAGCGGTGCGCAAAGCCGGTGGCAATGTCACCCGTGAGCCCGGCCCGGTAAAAGGCGGCAAAACAGTGATTGCCTTTGTGGAAGATCCGGACGGTTATAAAATCGAATTTATCGAAAACAAAAACGCACAAGCCGGATTAGGc                                                                                                                                                                         <  1:407682/146‑1 (MQ=255)                                                                                                                                                                  gCGAAGCGGTGCGCAAAGCCGGTGGCAATGTCACCCGTGAGCCCGGCCCGGTAAAAGGCGGCAAAACAGTGATTGCCTTTGTGGAAGATCCGGACGGTTATAAAATCGAATTTATCGAAAACAAAAACGCACAAGCCGGATTAGGCAACTAGTTCGCACCAAAAGTGCGGTAGAAAAtt                                                                                                                                        <  1:686168/179‑1 (MQ=255)                                                                                                                                                                          gTGCGCAAAGCCGGTGGCAATGTCACCCGTGAGCCCGGCCCGGTAAAAGGCGGCAAAACAGTGATTGCCTTTGTGGAAGATCCGGACGGTTATAAAATCGAATTTATCGAAAACAAAAACGCACAAGCCGGATTAGGCAACTAGTTCGCACCAAAAGTGCGGTAGAAAATTCAAACGTTTTTTAACGTGGATTATGAACGATTATCCACGTTTTATTTTTAGGATGAACTATGTCAGAAACCCAAGAAAc                                                         <  1:142377/250‑1 (MQ=255)                                                                                                                                                                          gTGCGCAAAGCCGGTGGCAATGTCACCCGTGAGCCCGGCCCGGTAAAAGGCGGCAAAACAGTGATTGCCTTTGTGGAAGATCCGGACGGTTATAAAATCGAATTTATCGAAAACAAAAACGCACAAGCCGGATTAGGCAACTAGTTCGCACCAAAAGTGCGGTAGAAAATTCAAACGTTTTTTAACGTGGATTATGAACGATTATCCACGTTTTATTTTTAGGATGAACTATGTCAGAAACCCAAGAAAc                                                         >  1:750905/1‑250 (MQ=255)                                                                                                                                                                          gTGCGCAAAGCCGGTGGCAATGTCACCCGTGAGCCCGGCCCGGTAAAAGGCGGCAAAACAGTGATTGCCTTTGTGGAAGATCCGGACGGTTATAAAATCGAATTTATCGAAAACAAAAACGCACAAGCCGGATTAGGCAACTAGTTCGCACCAAAAGTGCGGTAGAAAATTCAAACGTTTTTTAACGTGGATTATGAACGATTATCCACGTTTTATTTTTAGGATGAACTATGTCAGAAACCCAAGAAAc                                                         <  1:517382/250‑1 (MQ=255)                                                                                                                                                                          gTGCGCAAAGCCGGTGGCAATGTCACCCGTGAGCCCGGCCCGGTAAAAGGCGGCAAAACAGTGATTGCCTTTGTGGAAGATCCGGACGGTTATAAAATCGAATTTATCGAAAACAAAAACGCACAAGCCGGATTAGGCAACTAGTTCGCACCAAAAGTGCGGTAGAAAATTCAAACGTTTTTTAACGTGGATTATGAACGATTATCCACGTTTTATTTTTAGGATGAACTATGTCAGAAACCCAAGAAAc                                                         >  1:183506/1‑250 (MQ=255)                                                                                                                                                                          gTGCGCAAAGCCGGTGGCAATGTCACCCGTGAGCCCGGCCCGGTAAAAGGCGGCAAAACAGTGATTGCCTTTGTGGAAGATCCGGACGGTTATAAAATCGAATTTATCGAAAACAAAAACGCACAAGCCGGATTAGGCAACTAGTTCGCACCAAAAGTGCGGTAGAAAATTCAAACGTTTTTTAACGTGGATTATGAACGATTATCCACGTTTTATTTTTAGGATGAACTATGTCAGAAACCCAAGAAAc                                                         <  1:604922/250‑1 (MQ=255)                                                                                                                                                                           tGCGCAAAGCCGGTGGCAATGTCACCCGTGAGCCCGGCCCGGTAAAAGGCGGCAAAACAGTGATTGCCTTTGTGGAAGATCCGGACGGTTATAAAATCGAATTTATCGAAAACAAAAACGCACAAGCCGGATTAGGCAACTAGTTCGCACCAAAAGTGCGGTAGAAAATTCAAACGTTTTTTAACGTGGATTATGAACGATTATCCACGTTTTATTTTTAGGATGAACTATGTCAGAAACCCAAGAAAcc                                                        <  1:8789/250‑1 (MQ=255)                                                                                                                                                                            gcgcAAAGCCGGTGGCAATGTCACCCGTGAGCCCGGCCCGGTAAAAGGCGGCAAAACAGTGATTGCCTTTGTGGAAGATCCGGACGGTTATAAAATCGAATTTATCGAAAACAAAAACGCACAAGCCGGATTAGGCAACTAGTTCGCACCAAAAGTGCGGTAGAAAATTCAAACGTTTTTTAACGTGGATTATGAACGATTATCCACGTTTTATTTTTAGGATGAACTATGTCAGAAACCCAAGAAACCat                                                      >  1:250675/1‑251 (MQ=255)                                                                                                                                                                                              tGTCACCCGTGAGCCCGGCCCGGTAAAAGGCGGCAAAACAGTGATTGCCTTTGTGGAAGATCCGGACGGTTATAAAATCGAATTTATCGAAAACAAAAACGCACAAGCCGGATTAGGCAACTAGTTCGCACCAAAAGTGCGGTAGAAAATTCAAACGTTTTTTAACGTGGATTATGAACGATTATCCACGTTTTATTTTTAGGATGAACTATGTCAGAAACCCAAGAAACCATTAATTACAATATATTaaa                                    >  1:3949/1‑251 (MQ=255)                                                                                                                                                                                                tCACCCGTGAGCCCGGCCCGGTAAAAGGCGGCAAAACAGTGATTGCCTTTGTGGAAGATCCGGACGGTTATAAAATCGAATTTATCGAAAACAAAAACGCACAAGCCGGATTAGGCAACTAGTTCGCACCAAAAGTGCGGTAGAAAATTCAAACGTTTTTTAACGTGGATTATGAACGATTATCCACGTTTTATTTTTAGGATGAACTATGTCAGAAACCCAAGAAACCATTAATTACAATATATTaaaa                                   <  1:670991/250‑1 (MQ=255)                                                                                                                                                                                                tCACCCGTGAGCCCGGCCCGGTAAAAGGCGGCAAAACAGTGATTGCCTTTGTGGAAGATCCGGACGGTTATAAAATCGAATTTATCGAAAACAAAAACGCACAAGCCGGATTAGGCAACTAGTTCGCACCAAAAGTGCGGTAGAAAATTCAAACGTTTTTTAACGTGGATTATGAACGATTATCCACGTTTTATTTTTAGGATGAACTATGTCAGAAACCCAAGAAACCATTAATTACAATATATTAAAAc                                  <  1:811785/251‑1 (MQ=255)                                                                                                                                                                                                tCACCCGTGAGCCCGGCCCGGTAAAAGGCGGCAAAACAGTGATTGCCTTTGTGGAAGATCCGGACGGTTATAAAATCGAATTTATCGAAAACAAAAACGCACAAGCCGGATTAGGCAACTAGTTCGCACCAAAAGTGCGGTAGAAAATTCAAACGTTTTTTAACGTGGATTATGAACGATTATCCACGTTTTATTTTTAGGATGAACTATGTCAGAAACCCAAGAAACCATTAATTACAATATATTAAAAc                                  <  1:726561/251‑1 (MQ=255)                                                                                                                                                                                                tCACCCGTGAGCCCGGCCCGGTAAAAGGCGGCAAAACAGTGATTGCCTTTGTGGAAGATCCGGACGGTTATAAAATCGAATTTATCGAAAACAAAAACGCACAAGCCGGATTAGGCAACTAGTTCGCACCAAAAGTGCGGTAGAAAATTCAAACGTTTTTTAACGTGGATTATGAACGATTATCCACGTTTTATTTTTAGGATGAACTATGTCAGAAACCCAAGAAACCATTAATTACAATATATTAAAAc                                  <  1:716588/251‑1 (MQ=255)                                                                                                                                                                                                tCACCCGTGAGCCCGGCCCGGTAAAAGGCGGCAAAACAGTGATTGCCTTTGTGGAAGATCCGGACGGTTATAAAATCGAATTTATCGAAAACAAAAACGCACAAGCCGGATTAGGCAACTAGTTCGCACCAAAAGTGCGGTAGAAAATTCAAACGTTTTTTAACGTGGATTATGAACGATTATCCACGTTTTATTTTTAGGATGAACTATGTCAGAAACCCAAGAAACCATTAATTACAATATATTAAAAc                                  <  1:325597/251‑1 (MQ=255)                                                                                                                                                                                                tCACCCGTGAGCCCGGCCCGGTAAAAGGCGGCAAAACAGTGATTGCCTTTGTGGAAGATCCGGACGGTTATAAAATCGAATTTATCGAAAACAAAAACGCACAAGCCGGATTAGGCAACTAGTTCGCACCAAAAGTGCGGTAGAAAATTCAAACGTTTTTTAACGTGGATTATGAACGATTATCCACGTTTTATTTTCAGGATGAACTATGTCAGAAACCCAAGAAACCATTAATTACAATATATTAAAAc                                  <  1:813221/251‑1 (MQ=255)                                                                                                                                                                                                         aGCCCGGCCCGGTAAAAGGCGGCAAAACAGTGATTGCCTTTGTGGAAGATCCGGACGGTTATAAAATCGAATTTATCGAAAACAAAAACGCACAAGCCGGATTAGGCAACTAGTTCGCACCAAAAGTGCGGTAGAAAATTCAAACGTTTTTTAACGTGGATTATGAACGATTATCCACGTTTTATTTTTAGGATGAACTATGTCAGAAACCCAAGAAACCATTAATTACAATATATTAAAACATCGATTc                          <  1:213087/250‑1 (MQ=255)                                                                                                                                                                                                                 ccggTAAAAGGCGGCAAAACAGTGATTGCCTTTGTGGAAGATCCGGACGGTTATAAAATCGAATTTATCGAAAACAAAAACGCACAAGCCGGATTAGGCAACTAGTTCGCACCAAAAGTGCGGTAGAAAATTCAAACGTTTTTTAACGTGGATTATGAACGATTATCCAc                                                                                                  <  1:409254/170‑1 (MQ=255)                                                                                                                                                                                                                   ggTAAAAGGCGGCAAAACAGTGATTGCCTTTGTGGAAGATCCGGACGGTTATAAAATCGAATTTATCGAAAACAAAAACGCACAAGCCGGATTAGGCAACTAGTTCGCACCAAAAGTGCGGTAGAAAATTCAAACGTTTTTTAACGTGGATTATGAACGATTATCCACGTTTTATTTTTAGGATGAACTATGTCAGAAACCCAAGaaa                                                          >  1:757190/1‑208 (MQ=255)                                                                                                                                                                                                                                aaaaCAGTGATTGCCTTTGTGGAAGATCCGGACGGTTATAAAATCGAATTTATCGAAAACAAAAACGCACAAGCCGGATTAGGCAACTAGTTCGCACCAAAAGTGCGGTAGAAAATTCAAACGTTTTTTAACGTGGATTATGAACGATTATCCACGTTTTATTTTTAGGATGAACTATGTCAGAAACCCAAGAAACCATTAATTACAATATATTAAAACATCGATTCCGAGGTTATTTGCCGGTGGTAATc  <  1:617606/251‑1 (MQ=255)                                                                                                                                                                                                                                 aaaCAGTGATTGCCTTTGTGGAAGATCCGGACGGTTATAAAATCGAATTTATCGAAAACAAAAACGCACAAGCCGGATTAGGCAACTAGTTCGCACCAAAAGTGCGGTAGAAAATTCAAACGTTTTTTAACGTGGATTATGAACGATTATCCACGTTTTATTTTTAGGATGAAc                                                                              <  1:445864/174‑1 (MQ=255)                                                                                                                                                                                                                                    cAGTGATTGCCTTTGTGGAAGATCCGGACGGTTATAAAATCGAATTTATCGAAAACAAAAACGCACAAGCCGGATTAGGCAACTAGTTCGCACCAAAAGTGCGGTAGAAAATTCAAACGTTTTTTAACGTGGATTATGAACGATTATCCACGTTTTATTTTTAGGATGAACTATGt                                                                         <  1:739268/176‑1 (MQ=255)                                                                                                                                                                                                                                     aGTGATTGCCTTTGTGGAAGATCCGGACGGTTATAAAATCGAATTTATCGAAAACAAAAACGCACAAGCCGGATTAGGCAACTAGTTCGCACCAAAAGTGCGGTAGAAAATTCAAACGTTTTTTAACGTGGATTATGAACGATTATCCACGTTTTATTTTTAGGATGAACTATGt                                                                         >  1:402188/1‑175 (MQ=255)                                                                                                                                                                                                                                      gTGATTGCCTTTGTGGAAGATCCGGACGGTTATAAAATCGAATTTATCGAAAACAAAAACGCACAAGCCGGATTAGGCAACTAGTTCGCACCAAAAGTGCGGTAGAAAATTCAAACGTTTTTTAACGTGGATTATGaa                                                                                                             <  1:445554/138‑1 (MQ=255)                                                                                                                                                                                                                                      gTGATTGCCTTTGTGGAAGATCCGGACGGTTATAAAATCGAATTTATCGAAAACAAAAACGCACAAGCCGGATTAGGCAACTAGTTCGCACCAAAAGTGCGGTAGAAAATTCAAACGTTTTTTAACGTGGATTATGaa                                                                                                             <  1:809284/138‑1 (MQ=255)                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                               CGAAAATCCCGAATACAAATATTCTCTGGCATTTTTAGGTTACGACGATGAAGACAAAACCTCCGTGTTGGAACTGACTTACAACTGGGGCGTGGATAAATACGAACTCGGCACCGCTTACGGGCATATCGCCATCGGCACGGACGATATTTACGCCACCTGCGAAGCGGTGCGCAAAGCCGGTGGCAATGTCACCCGTGAGCCCGGCCCGGTAAAAGGCGGCAAAACAGTGATTACCTTTGTGGAAGATCCGGACGGTTATAAAATCGAATTTATCGAAAACAAAAACGCACAAGCCGGATTAGGCAACTAGTTCGCACCAAAAGTGCGGTAGAAAATTCAAACGTTTTTTAACGTGGATTATGAACGATTATCCACGTTTTATTTTTAGGATGAACTATGTCAGAAACCCAAGAAACCATTAATTACAATATATTAAAACATCGATTCCGAGGTTATTTGCCGGTGGTAATC  >  1/2018290‑2018763

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑