Predicted mutation
evidence seq id position mutation annotation gene description
RA 1 2,009,063 C→A R323L (CGT→CTT)  unknown ← Aspartyl‑tRNA synthetase (EC 6.1.1.12)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*12,009,0630CA100.0% 184.6 / NA 64R323L (CGT→CTT) unknownAspartyl‑tRNA synthetase (EC 6.1.1.12)
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (25/39);  total (25/39)

ATCTGTGGCGGTTTGCCATTTGTCGGCACCGAAGAATAAAATATCGCCAGTTTGCGCGCCCACGCGTTCAAACAAGGCTTTTAGCACGTCTTCATTTAAGAATTTTGCAATAGGGCTTTGGACGCCTTCAAGACCGGCGTTTACGTCATTCACTTTTAACCAAGCCAAGCCTTTAGCACCATAAATGCCGACAAATTGGGTATATTCATCAATTTGTTTACGGGTGATTTCATTCCCCTGCGGCACACGAATTAAGGTGACACGACCATTCGGGTCATTTGCCGGACCTTGGAATATCTTGAAATCTACGTTTTTGACAATATCTGCCACGTCCACCAGTTCTAACGGATTGCGCAAATCCGGTTTATCGGAACCAAAACGGCGCATCGCTTCTTGCCAGGTTATCACCGGGAATTTGCCCAAATCGACACCTAAAGTATGCTGCCACAAACCGTGCACCATACGTTCCATGATTTCACGTACC  >  1/2008816‑2009299
                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                            
aTCTGTGGCGGTTTGCCATTTGTCGGCACCGAAGAATAAAATATCGCCAGTTTGCGCGCCCACGCGTTCAAACAAGGCTTTTAGCACGTCTTCATTTAAGAATTTTGCAATAGGGCTTTGGACGCCTTCAAGACCGGCGTTTACGTCATTCACTTTTAACCAAGCCAAGCCTTTAGCACCATAAATGCCGACAAATTGGGTATATTCATCAATTTGTTTACGGGTGATTTCATTCCCCTGCGGCACAAGaa                                                                                                                                                                                                                                           <  1:162839/251‑1 (MQ=255)
       gCGGTTTGCCATTTGTCGGCACCGATGAATAAAATATCGCCAGTTTGCGCGCCCACGCGTTCAAACAAGGCTTTTAGCACGTCTTCATTTAAGAATTTTGCAATAGGGCTTTGGACGCCTTCAAGACCGGCGTTTACGTCATTCACTTTTAACCAAGCCAAGCCTTTAGCACCATAAATGCCGACAAATTGGGTATATTCATCAATTTGTTTACGGGTGATTTCATTCCCCTGCGGCACAAGAATTAAgg                                                                                                                                                                                                                                     <  1:484931/250‑1 (MQ=255)
        cGGTTTGCCATTTGTCGGCACCGAAGAATAAAATATCGCCAGTTTGCGCGCCCACGCGTTCAAACAAGGCTTTTAGCACGTCTTCATTTAAGAATTTTGCAATAGGGCTTTGGACGCCTTCAAGACCGGCGTTTACGTCATTCACTTTTAACCAAGCCAAGCCTTTAGCACCATAAATGCCGACAAATTGGGTATATTCATCAATTTGTTTACGGGTGATTTCATTCCCCTGCGGCACAAGAATTAAGGt                                                                                                                                                                                                                                    <  1:219553/250‑1 (MQ=255)
                                 gaaTAAAATATCGCCAGTTTGCGCGCCCACGCGTTCAAACAAGGCTTTTAGCACGTCTTCATTTAAGAATTTTGCAATAGGGCTTTGGACGCCTTCAAGACCGGCGTTTACGTCATTCACTTTTAACCAAGCCAAGCCTTTAGCACCATAAATGCCGACAAATTGGGTATATTCATCAATTTGTTTACGGGTGATTTCATTCCCCTGCGGCACAAGAATTAAGGTGACACGACCATTCGGGTCATTTGCCg                                                                                                                                                                                                          >  1:836301/1‑251 (MQ=255)
                                           tCGCCAGTTTGCGCGCCCACGCGTTCAAACAAGGCTTTTAGCACGTCTTCATTTAAGAATTTTGCAATAGGGCTTTGGACGCCTTCAAGACCGGCGTTTACGTCATTCACTTTTAACCAAGCCAAGCCTTTAGCACCATAAATGCCGACAAATTGGGTATATTCATCAATTTGTTTACGGGTGATTTCATTCCCCTGCGGCACAAGAATTAAGGTGACACGACCATTCGGGTCATTTGCCGGACCTTGGaa                                                                                                                                                                                                <  1:851856/251‑1 (MQ=255)
                                            cGCCAGTTTGCGCGCCCACGCGTTCAAACAAGGCTTTTAGCACGTCTTCATTTAAGAATTTTGCAATAGGGCTTTGGACGCCTTCAAGACCGGCGTTTACGTCATTCACTTTTAACCAAGCCAAGCCTTTAGCACCATAAATGCCGACAAATTGGGTATATTCATCAATTTGTTTACGGGTGATTTCATTCCCCTGCGGCACAAGAATTAAGGTGACACGACCATTCGGGTCATTTGCCGGACCTTGGAat                                                                                                                                                                                               <  1:608615/251‑1 (MQ=255)
                                                      cgcgcCCACGCGTTCAAACAAGGCTTTTAGCACGTCTTCATTTAAGAATTTTGCAATAGGGCTTTGGACGCCTTCAAGACCGGCGTTTACGTCATTCACTTTTAACCAAGCCAAGCCTTTAGCACCATAAATGCCGACAAATTGGGTATATTCATCAATTTGTTTACGGGTGATTTCATTCCCCTGCGGCACAAGAATTAAGGTGACACGACCATTCGGGTCATTTGCCGGACCTTGGAATATCTTGaaa                                                                                                                                                                                      <  1:649492/250‑1 (MQ=255)
                                                          cccACGCGTTCAAACAAGGCTTTTAGCACGTCTTCATTTAAGAATTTTGCAATAGGGCTTTGGACGCCTTCAAGACCGGCGTTTACGTCATTCACTTTTAACCAAGCCAAGCCTTTAGCACCATAAATGCCGACAAATTGGGTATATTCATCAATTTGTTTACGGGTGATTTCATTCCCCTGCGGCACAAGAATTAAGGTGACACGACCATTCGGGTCATTTGCCGGACCTTGGAATATCTTGAAATCTAc                                                                                                                                                                                 <  1:684577/251‑1 (MQ=255)
                                                                cgTTCAAACAAGGCTTTTAGCACGTCTTCATTTAAGAATTTTGCAATAGGGCTTTGGACGCCTTCAAGACCGGCGTTTACGTCATTCACTTTTAACCAAGCCAAGCCTTTAGCACCATAAATGCCGACAAATTGGGTATATTCATCAATTTGTTTACGGGTGATTTCATTCCCCTGCGGCACAAGaa                                                                                                                                                                                                                                           <  1:648952/187‑1 (MQ=255)
                                                                           ggCTTTTAGCACGTCTTCATTTAAGAATTTTGCAATAGGGCTTTGGACGCCTTCAAGACCGGCGTTTACGTCATTCACTTTTAACCAAGCCAAGCCTTTAGCACCATAAATGCCGACAAATTGGGTATATTCATCAATTTGTTTACGGGTGATTTCATTCCCCTGCGGCACAAGAATTAAGGTGACACGACCATTCGGGTCATTTGCCGGACCTTGGAATATCTTGAAATCTACGTTTTTGACAatat                                                                                                                                                                   >  1:190797/1‑248 (MQ=255)
                                                                            gCTTTTAGCACGTCTTCATTTAAGAATTTTGCAATAGGGCTTTGGACGCCTTCAAGACCGGCGTTTACGTCATTCACTTTTAACCAAGCCAAGCCTTTAGCACCATAAATGCCGACAAATTGGGTATATTCATCAATTTGTTTACGGGTGATTTCATTCCCCTGCGGCACAAGAATTAAGGTGACACGACCATTCGGGTCATTTGCCGGACCTTGGAATATCTTGAAATCTACGTTTTTGACAATATCTg                                                                                                                                                                >  1:603315/1‑250 (MQ=255)
                                                                                       gTCTTCATTTAAGAATTTTGCAATAGGGCTTTGGACGCCTTCAAGACCGGCGTTTACGTCATTCACTTTTAACCAAGCCAAGCCTTTAGCACCATAAATGCCGACAAATTGGGTATATTCATCAATTTGTTTACGGGTGATTTCATTCCCCTGCGGCACAAGAATTAAGGTGACACGACCATTCGGGTCATTTGCCGGACCTTGGAATATCTTGAAATCTACg                                                                                                                                                                                >  1:846561/1‑223 (MQ=255)
                                                                                              tttAAGAATTTTGCAATAGGGCTTTGGACGCCTTCAAGACCGGCGTTTACGTCATTCACTTTTAACCAAGCCAAGCCTTTAGCACCATAAATGCCGACAAATTGGGTATATTCATCAATTTGTTTACGGGTGATTTCATTCCCCTGCGGCACAAGAATTAAGGTGACACGACCATTCGGGTCATTTGCCGGACCTTGGAATATCTTGAAATCTACGTTTTTGACAATATCTGCCACGTCCACCAGTTCTaa                                                                                                                                             >  1:388400/1‑251 (MQ=255)
                                                                                               ttAAGAATTTTGCAATAGGGCTTTGGACGCCTTCAAGACCGGCGTTTACGTCATTCACTTTTAACCAAGCCAAGCCTTTAGCACCATAAATGCCGACAAATTGGGTATATTCATCAATTTGTTTACGGGTGATTTCATTCCCCTGCGGCACAAGAATTAAGGTGACACGACCATTCGGGTCATTTGCCGGACCTTGGAATATCTTGAAATCTACGTTTTTGACAATATCTGCCACGTCCACCAGTTCTAAc                                                                                                                                            <  1:85410/251‑1 (MQ=255)
                                                                                                 aaGAATTTTGCAATAGGGCTTTGGACGCCTTCAAGACCGGCGTTTACGTCATTCACTTTTAACCAAGCCAAGCCTTTAGCACCATAAATGCCGACAAATTGGGTATATTCATCAATTTGTTTACGGGTGATTTCATTCCCCTGCGGCACAAGAATTAAGGTGACACGACCATTCGGGTCATTTGCCGGACCTTGGAATATCTTGAAATCTACGTTTTTGACAATATCTGCCACGTCCACCAGTTCTAACgg                                                                                                                                          <  1:715648/251‑1 (MQ=255)
                                                                                                          gCAATAGGGCTTTGGACGCCTTCAAGACCGGCGTTTACGTCATTCACTTTTAACCAAGCCAAGCCTTTAGCACCATAAATGCCGACAAATTGGGTATATTCATCAATTTGTTTACGGGTGATTTCATTCCCCTGCGGCACAAGAATTAAGGTGACACGACCATTCGGGTCATTTGc                                                                                                                                                                                                            >  1:675440/1‑176 (MQ=255)
                                                                                                                  gCTTTGGACGCCTTCAAGACCGGCGTTTACGTCATTCACTTTTAACCAAGCCAAGCCTTTAGCACCATAAATGCCGACAAATTGGGTATATTCATCAATTTGTTTACGGGTGATTTCATTCCCCTGCGGCACAAGAATTAAGGTGACACGACCATTCGGGTCATTTGCCGGACCTTGGAATATCTTGAAATCTACGtttt                                                                                                                                                                            <  1:333948/200‑1 (MQ=255)
                                                                                                                       ggACGCCTTCAAGACCGGCGTTTACGTCATTCACTTTTAACCAAGCCAAGCCTTTAGCACCATAAATGCCGACAAATTGGGTATATTCATCAATTTGTTTACGGGTGATTTCATTCCCCTGCGGCACAAGAATTAAGGTGACACGACCATTc                                                                                                                                                                                                                       <  1:225096/152‑1 (MQ=255)
                                                                                                                            ccTTCAAGACCGGCGTTTACGTCATTCACTTTTAACCAAGCCAAGCCTTTAGCACCATAAATGCCGACAAATTGGGTATATTCATCAATTTGTTTACGGGTGATTTCATTCCCCTGCGGCACAAGAATTAAGGTGACACGACCATTCGGGTCa                                                                                                                                                                                                                 <  1:294501/153‑1 (MQ=255)
                                                                                                                            ccTTCAAGACCGGCGTTTACGTCATTCACTTTTAACCAAGCCAAGCCTTTAGCACCATAAATGCCGACAAATTGGGTATATTCATCAATTTGTTTACGGGTGATTTCATTCCCCTGCGGCACAAGAATTAAGGTGACACGACCATTCGGGTCATTTGCCGGACCTTGGAATATCTTGAAATCTACGTTTTTGACAATATCTGCCACGTCCACCAGTTCTaa                                                                                                                                             <  1:396197/221‑1 (MQ=255)
                                                                                                                             cTTCAAGACCGGCGTTTACGTCATTCACTTTTAACCAAGCCAAGCCTTTAGCACCATAAATGCCGACAAATTGGGTATATTCATCAATTTGTTTACGGGTGATTTCATTCCCCTGCGGCACAAGAATTAAgg                                                                                                                                                                                                                                     >  1:649980/1‑132 (MQ=255)
                                                                                                                              ttCAAGACCGGCGTTTACGTCATTCACTTTTAACCAAGCCAAGCCTTTAGCACCATAAATGCCGACAAATTGGGTATATTCATCAATTTGTTTACGGGTGATTTCATTCCCCTGCGGCACAAGAATTAAGGTGACACGACCATTCGGGTCATTTGCCGGACCTTGGAATATCTTGAAATCTACGTTTTTGACAATATCTGCCACGTCCACCAGTTCTAACGGATTGCGCAAATCCGGTTTATCGGAACCaa                                                                                                             >  1:887259/1‑251 (MQ=255)
                                                                                                                                cAAGACCGGCGTTTACGTCATTCACTTTTAACCAAGCCAAGCCTTTAGCACCATAAATGCCGACAAATTGGGTATATTCATCAATTTGTTTACGGGTGATTTCATTCCCCTGCGGCACAAGAATTAAGGTGACACGACCATTCGGGTCATTTGCCGGACCTTGGAATATCTTGAAATCTACGTTTTTGACAATATCTGCCACGTCCACCAGTTCTAACGGATTGCGCAAATCCGGTTTATCGGAACCaaaa                                                                                                           <  1:293540/251‑1 (MQ=255)
                                                                                                                                        gCGTTTACGTCATTCACTTTTAACCAAGCCAAGCCTTTAGCACCATAAATGCCGACAAATTGGGTATATTCATCAATTTGTTTACGGGTGATTTCATTCCCCTGCGGCACAAGAATTAAGGTGACACGACCATTCGGGTCATTTGCCGGACCTTGGAATATCTTGAAATCTAc                                                                                                                                                                                 >  1:224308/1‑173 (MQ=255)
                                                                                                                                               cGTCATTCACTTTTAACCAAGCCAAGCCTTTAGCACCATAAATGCCGACAAATTGGGTATATTCATCAATTTGTTTACGGGTGATTTCATTCCCCTGCGGCACAAGAATTAAGGTGACACGACCATTCGGGTCATTTGCCgg                                                                                                                                                                                                         >  1:616811/1‑142 (MQ=255)
                                                                                                                                                gTCATTCACTTTTAACCAAGCCAAGCCTTTAGCACCATAAATGCCGACAAATTGGGTATATTCATCAATTTGTTTACGGGTGATTTCATTCCCCTGCGGCACAAGAATTAAGGTGACACGACCATTCGGGTCATTTGCCGGACCTTGGAATATCTTGAAATCTACGTTTTTGACAATATCTGCCACGTCCACCAGTTCTAACGGATTGCGCAAATCCGGTTTATCGGAACCAAAACGGCGCATCGcttctt                                                                                           >  1:18394/1‑251 (MQ=255)
                                                                                                                                                        cTTTTAACCAAGCCAAGCCTTTAGCACCATAAATGCCGACAAATTGGGTATATTCATCAATTTGTTTACGGGTGATTTCATTCCCCTGCGGCACAAGAATTAAGGTGACACGACCATTCGGGTCATTTGCCGGACCTTGGAATATCTTGaaa                                                                                                                                                                                      <  1:499323/152‑1 (MQ=255)
                                                                                                                                                        cTTTTAACCAAGCCAAGCCTTTAGCACCATAAATGCCGACAAATTGGGTATATTCATCAATTTGTTTACGGGTGATTTCATTCCCCTGCGGCACAAGAATTAAGGTGACACGACCATTCGGGTCATTTGCCGGACCTTGGAATATCTTGAAATCTACGTTTTTGACAATATCTGCCACGt                                                                                                                                                          <  1:358447/180‑1 (MQ=255)
                                                                                                                                                        cTTTTAACCAAGCCAAGCCTTTAGCACCATAAATGCCGACAAATTGGGTATATTCATCAATTTGTTTACGGGTGATTTCATTCCCCTGCGGCACAAGAATTAAGGTGACACGACCATTCGGGTCATTTGCCGGACCTTGGAATATCTTGAAATCTACGTTTTTGACAATATCTGCCACGTCCACCAGTTCTAACGGATTGCGCAAATCCGGTTTATCGGAACCAAAACGGCGCATCGCTTCTTGCCAGGtt                                                                                   <  1:254280/251‑1 (MQ=255)
                                                                                                                                                        cTTTTAACCAAGCCAAGCCTTTAGCACCATAAATGCCGACAAATTGGGTATATTCATCAATTTGTTTACGGGTGATTTCATTCCCCTGCGGCACAAGAATTAAGGTGACACGACCATTCGGGTCATTTGCCGGACCTTGGAATATCTTGAAATCTACGTTTTTGACAATATCTGCCACGTCCACCAGTTCTAACGGATTGCGCAAATCCGGTTTATCGGAACCAAAACGGCGCATCGCTTCTTGCCAGGtt                                                                                   >  1:889362/1‑251 (MQ=255)
                                                                                                                                                                 aagccaagccTTTAGCACCATAAATGCCGACAAATTGGGTATATTCATCAATTTGTTTACGGGTGATTTCATTCCCCTGCGGCACAAGAATTAAGGTGACACGACCATTCGGGTCATTTGCCGGACCTTGGAATATCTTGAAATCTACGTTTTTGACAATATCTGCCACGTCCACCAGTTCTAACGGATTGCGCAAATCCGGTTTATCGGAACCAAAACGGCGCATCGCTTCTTGCCAGGTTATCAccctg                                                                          <  1:557973/251‑4 (MQ=255)
                                                                                                                                                                  agccaagccTTTAGCACCATAAATGCCGACAAATTGGGTATATTCATCAATTTGTTTACGGGTGATTTCATTCCCCTGCGGCACAAGAATTAAGGTGACACGACCATTCGGGTCATTTGCCGGACCTTGGAATATCTTGAAATCTACGTTTTTGACAATATCTGCCACGTCCACCAGTTc                                                                                                                                                <  1:662815/180‑1 (MQ=255)
                                                                                                                                                                         ccTTTAGCACCATAAATGCCGACAAATTGGGTATATTCATCAATTTGTTTACGGGTGATTTCATTCCCCTGCGGCACAAGAATTAAGGTGACACGACCATTCGGGTCATTTGCCGGACCTTGGAATATCTTGAAATCTACGTTTTTGACAATATCTGCCACGTCCACCAGTTCTAACGGATTGCGCAAATCCGGTTTATCGGAACCAAAACGGCGCATCGCTTCTTGcc                                                                                        <  1:354889/229‑1 (MQ=255)
                                                                                                                                                                         ccTTTAGCACCATAAATGCCGACAAATTGGGTATATTCATCAATTTGTTTACGGGTGATTTCATTCCCCTGCGGCACAAGAATTAAGGTGACACGACCATTCGGGTCATTTGCCGGACCTTGGAATATCTTGAAATCTACGTTTTTGACAATATCTGCCACGTCCACCAGTTCTAACGGATTGCGCAAATCCGGTTTATCGGAACCAAAACGGCGCATCGCTTCTTGCCAGGTTATCAc                                                                              >  1:609406/1‑239 (MQ=255)
                                                                                                                                                                          cTTTAGCACCATAAATGCCGACAAATTGGGTATATTCATCAATTTGTTTACGGGTGATTTCATTCCCCTGCGGCACAAGAATTAAGGTGACACGACCATTCGGGTCATTTGCCGGACCTTGGAATATCTTGAAATCTACGTTTTTGACAATATCTGCCACGTCCACCAGTTCTAACGGATTGCGCAAATCCGGTTTATCGGAACCAAAACGGCGCATCGCTTCTTGCCAGGTTATCACCGGGaa                                                                        <  1:737020/244‑1 (MQ=255)
                                                                                                                                                                                  ccATAAATGCCGACAAATTGGGTATATTCATCAATTTGTTTACGGGTGATTTCATTCCCCTGCGGCACAAGAATTAAGGTGACACGACCATTCGGGTCATTTGCCGGACCTTGGAATATCTTGAAATTTACGTTTTTGACAATATCTGCCACGTCCACCAGTTCTAACGGATTGCGCAAATCCGGTTTATCGGAACCAAAACGGCGCATCGCTTCTTGCCAGGTTATCACCGGGAATTTGCCCAAATCGa                                                          >  1:569924/1‑250 (MQ=255)
                                                                                                                                                                                  ccATAAATGCCGACAAATTGGGTATATTCATCAATTTGTTTACGGGTGATTTCATTCCCCTGCGGCACAAGAATTAAGGTGACACGACCATTCGGGTCATTTGCCGGACCTTGGAATATCTTGAAATCTACGTTTTTGACAATATCTGCCACGTCCACCAGTTCTAACGGATTGCGCAAATCCGGTTTATCgg                                                                                                                   >  1:376628/1‑193 (MQ=255)
                                                                                                                                                                                  ccATAAATGCCGACAAATTGGGTATATTCATCAATTTGTTTACGGGTGATTTCATTCCCCTGCGGCACAAGAATTAAGGTGACACGACCATTCGGGTCATTTGCCGGACCTTGGAATATCTTGAAATCTACGTTTTTGACAATATCTGCCACGTCCACCAGTTCTAACGGATTGCGCAAATCCGGTTTATCGGAACCAAAACGGCGCATCGCTTCTTGCCAGGTTATCACCGGGAATTTGCCCAAATCGa                                                          >  1:626161/1‑250 (MQ=255)
                                                                                                                                                                                           ccGACAAATTGGGTATATTCATCAATTTGTTTACGGGTGATTTCATTCCCCTGCGGCACAAGAATTAAGGTGACACGACCATTCGGGTCATTTGCCGGACCTTGGAATATCTTGAAATCTACGTTTTTGACAATATCTGCCACGTCCACCAGTTCTAACGGATTGCGCAAATCCGGTTTATCGGAACCAAAACGGCGCATCGCTTCTTGCCAGGTTATCACCGGGAATTTGCCCAAATCGACACCTAAAGt                                                <  1:854310/251‑1 (MQ=255)
                                                                                                                                                                                            cGACAAATTGGGTATATTCATCAATTTGTTTACGGGTGATTTCATTCCCCTGCGGCACAAGAATTAAGGTGACACGACCATTCGGGTCATTTGCCGGACCTTGGAATATCTTGAAATCTACGTTTTTGACAATATCTGCCACGTCCACCAGTTCTAACGGATTGCGCAAATCCGGTTTATCGGAACCAAAACGGCGCATCGCTTCTTGCCAGGTTATCACCGGGAATTTGCCCAAATCGACACCTAAAGTa                                               <  1:360708/251‑1 (MQ=255)
                                                                                                                                                                                            cGACAAATTGGGTATATTCATCAATTTGTTTACGGGTGATTTCATTCCCCTGCGGCACAAGAATTAAGGTGACACGACCATTCGGGTCATTTGCCGGACCTTGGAATATCTTGAAATCTACGTTTTTGACAATATCTGCCACGTCCACCAGTTCTAACGGATTGCGCAAATCCGGTTTATCGGAACCAAAACGGCGCATCGCTTCTTGCCAGGTTATCACCGGGAATTTGCCCAAATCGACACCTAAAGTa                                               <  1:519517/251‑1 (MQ=255)
                                                                                                                                                                                                     gggTATATTCATCAATTTGTTTACGGGTGATTTCATTCCCCTGCGGCACAAGAATTAAGGTGACACGACCATTCGGGTCATTTGCCGGACCTTGGAATATCTTGAAATCTACGTTTTTGACAATATCTGCCACGTCCACCAGTTCTAACGGATTGCGCAAATCCGGTTTATCGGAACCAAAACGGCGCATCGCTTCTTGCCAGGTTATCACCGGGAATTTGCCCAAATCGACACCTAAAGTATGCTGCca                                       >  1:109062/1‑250 (MQ=255)
                                                                                                                                                                                                      ggTATATTCATCAATTTGTTTACGGGTGATTTCATTCCCCTGCGGCACAAGAATTAAGGTGACACGACCATTCGGGTCATTTGCCGGACCTTGGAATATCTTGAAATCTACGTTTTTGACAATATCTGCCACGTCCACCAGTTCTAACg                                                                                                                                           >  1:113217/1‑149 (MQ=255)
                                                                                                                                                                                                         atatTCATCAATTTGTTTACGGGTGATTTCATTCCCCTGCGGCACAAGAATTAAGGTGACACGACCATTCGGGTCATTTGCCGGACCTTGGAATATCTTGAAATCTACGTTTTTGACAATATCTGCCACGTCCACCAGTTCTAACGGATTGCGCAAATCCGGTTTATCGGAACCAAAACGGCGCATCGCTTCTTGCCAGGTTATCACCGGGAATTTGCCCAAATCGACACCTAAAGTATGCTGCCACAAAc                                  <  1:338642/251‑1 (MQ=255)
                                                                                                                                                                                                         atatTCATCAATTTGTTTACGGGTGATTTCATTCCCCTGCGGCACAAGAATTAAGGTGACACGACCATTCGGGTCATTTGCCGGACCTTGGAATATCTTGAAATCTACGTTTTTGACAATATCTGCCACGTCCACCAGTTCTAACGGATTGCGCAAATCCGGTTTATCGGAACCAAAACGGCGCATCGCTTCTTGCCAGGTTATCACCGGGAATTTGCCCAAATCGACACCTAAAGTATGCTGCCACAAAc                                  <  1:344313/251‑1 (MQ=255)
                                                                                                                                                                                                           atTCATCAATTTGTTTACGGGTGATTTCATTCCCCTGCGGCACAAGAATTAAGGTGACACGACCATTCGGGTCATTTGCCGGACCTTGGAATATCTTGAAATCTACGTTTTTGACAATATCTGCCACGTCCACCAGTTCTAACGGATTGCGCAAATCCGGTTTATCGGAACCAAAACGGCGCATCGCTTCTTGCCAGGTTATCACCGGGAATTTGCCCAAATCGACACCTAAAGTATGCTGCCACAAACCg                                >  1:34603/1‑251 (MQ=255)
                                                                                                                                                                                                                tcaATTTGTTTACGGGTGATTTCATTCCCCTGCGGCACAAGAATTAAGGTGACACGACCATTCGGGTCATTTGCCGGACCTTGGAATATCTTGAAATCTACGTTTTTGACAATATCTGCCACGTCCACCAGTTCTAACGGATTGCGCAAATCCGGTTTATCGGAACCAAAACGGCGCATCGCTTCTTGCCAGGTTATCACCGGGAATTTGCCCAAATCGACACCTAAAGTATGCTGCCACAAACCGTGCAc                           <  1:307566/251‑1 (MQ=255)
                                                                                                                                                                                                                   aTTTGTTTACGGGTGATTTCATTCCCCTGCGGCACAAGAATTAAGGTGACACGACCATTCGGGTCATTTGCCGGACCTTGGAATATCTTGAAATCTACGTTTTTGACAATATCTGCCACGTCCACCAGTTCTAACGGATTGCGCAAATCCGGTTTATCGGAACCAAAACGGCGCATCGCTTCTTGCCAgg                                                                                     <  1:427760/190‑1 (MQ=255)
                                                                                                                                                                                                                       gTTTACGGGTGATTTCATTCCCCTGCGGCACAAGAATTAAGGTGACACGACCATTCGGGTCATTTGCCGGACCTTGGAATATCTTGAAATCTACGTTTTTGACAATATCTGCCACGTCCACCAGTTCTAACGGATTGCGCAAATCCGGTTTATCGGAACCAAAACGGCGCATCGCTTCTTGCCAGGTTATCACCGGGAAtt                                                                      >  1:526047/1‑201 (MQ=255)
                                                                                                                                                                                                                       gTTTACGGGTGATTTCATTCCCCTGCGGCACAAGAATTAAGGTGACACGACCATTCGGGTCATTTGCCGGACCTTGGAATATCTTGAAATCTACGTTTTTGACAATATCTGCCACGTCCACCAGTTCTAACGGATTGCGCAAATCCGGTTTATCGGAACCAAAACGGCGCATCGCTTCTTGCCAGGTTATCACCGGGAATTTGCCCAAATCGACACCTAAAGTa                                               >  1:515029/1‑224 (MQ=255)
                                                                                                                                                                                                                        tttACGGGTGATTTCATTCCCCTGCGGCACAAGAATTAAGGTGACACGACCATTCGGGTCATTTGCCGGACCTTGGAATATCTTGAAATCTACGTTTTTGACAATATCTGCCACGTCCACCAGTTCTAACGGATTGCGCAAATCCGGTTTATCGGAACCAAAACGGCGCATCGCTTCTTGCCAGGTTATCACCGGGAATTTGCCCAAATCGACACCTAAAGTATGCTGCCACAAACCGTGCACCATACGt                    <  1:747265/250‑1 (MQ=255)
                                                                                                                                                                                                                          tACGGGTGATTTCATTCCCCTGCGGCACAAGAATTAAGGTGACACGACCATTCGGGTCATTTGCCGGACCTTGGAATATCTTGAAATCTACGTTTTTGACAATATCTGCCACGTCCACCAGTTCTAACGGATTGCGCAAATCCGGTTTATCGGAACCAAAACGGCGCATCGCTTCTTGCCAGGTTATCACCGGGAATTTGCCCAAATCGACACCTAAAGTATGCTGCCACAAACCGTGCACCATACGTTcc                 <  1:186910/251‑1 (MQ=255)
                                                                                                                                                                                                                                  aTTTCATTCCCCTGCGGCACAAGAATTAAGGTGACACGACCATTCGGGTCATTTGCCGGACCTTGGAATATCTTGAAATCTACGTTTTTGACAATATCTGCCACGTCCACCAGTTCTAACGGATTGCGCAAATCCGGTTTATCGGAACCAAAACGGCGCATCGCTTCTTGCCAGGTTATCACCGGGAATTTGCCCAAATCGACACCTAAAGTATGCTGCCACAAAc                                  <  1:534789/226‑1 (MQ=255)
                                                                                                                                                                                                                                   tttCATTCCCCTGCGGCACAAGAATTAAGGTGACACGACCATTCGGGTCATTTGCCGGACCTTGGAATATCTTGAAATCTACGTTTTTGACAATATCTGcc                                                                                                                                                              <  1:145218/101‑1 (MQ=255)
                                                                                                                                                                                                                                     tCATTCCCCTGCGGCACAAGAATTAAGGTGACACGACCATTc                                                                                                                                                                                                                       >  1:536030/1‑42 (MQ=255)
                                                                                                                                                                                                                                      cATTCCCCTGCGGCACAAGAATTAAGGTGACACGACCATTCGGGTCATTTGCCGGACCTTGGAATATCTTGAAATCTACGTTTTTGACAATATCTGCCACGTCCACCAGTTCTAACGGATTGCGCAAATCCGGTTTATCGGAACCAAAACGGCGCATCGCTTCTTGCCAGGTTATCACCGGGAATTTGCCCAAATCGACACCTAAAGTATGCTGCCACAAACCGTGCACCATACGTTCCATGATTTCACGt     <  1:362655/251‑1 (MQ=255)
                                                                                                                                                                                                                                      cATTCCCCTGCGGCACAAGAATTAAGGTGACACGACCATTCGGGTCATTTGCCGGACCTTGGAATATCTTGAAATCTACGTTTTTGACAATATCTGCCACGTCCACCAGTTCTAACGGATTGCGCAAATCCGGTTTATCGGAACCAAAACGGCGCATCGCTTCTTGCCAGGTTATCACCGGGAATTTGCCCAAATCGACACCTAAAGTATGCTGCCACAAACCGTGCACCATACGTTCCATGATTTCACGt     <  1:527951/251‑1 (MQ=255)
                                                                                                                                                                                                                                      cATTCCCCTGCGGCACAAGAATTAAGGTGACACGACCATTCGGGTCATTTGCCGGACCTTGGAATATCTTGAAATCTACGTTTTTGACAATATCTGCCACGTCCACCAGTTCTAACGGATTGCGCAAATCCGGTTTATCGGAACCAAAACGGCGCATCGCTTCTTGCCAGGTTATCACCGGGAATTTGCCCAAATCGACACCTAAAGTATGCTGCCACAAACCGTGCACCATACGTTCCATGATTTCACGt     <  1:6384/251‑1 (MQ=255)
                                                                                                                                                                                                                                      cATTCCCCTGCGGCACAAGAATTAAGGTGACACGACCATTCGGGTCATTTGCCGGACCCTGGAATATCTTGAAATCTACGTTTTTGACAATATCTGCCACGTCCACCAGCTCTAACGGATTGCGCAAATCCGGTTTATCGGAACCAAAACGGCGCATCGCTTCTTGCCAGGTTATCACCGGGAATTTGCCCAAATCGACACCTAAAGTATGCTGCCACAAACCGTGCACCATACGtt                   >  1:633027/1‑237 (MQ=255)
                                                                                                                                                                                                                                         tCCCCTGCGGCACAAGAATTAAGGTGACACGACCATTCGGGTCATTTGCCGGACCTTGGAATATCTTGAAATCTACGTTTTTGACAATATCTGCCACGTCCACCAGTTCTAACGGATTGCGCAAATCCGGTTTATCGGAACCAAAACGGCGCATCGCTTCTTGCCAGGTTATCACCGGGAATTTGCCCAAATCGACACCTAAAGTATGCTGCCACAAACCGTGCACCATACGTTCCATGATTTCACGTAcc  <  1:842137/251‑1 (MQ=255)
                                                                                                                                                                                                                                         tCCCCTGCGGCACAAGAATTAAGGTGACACGACCATTCGGGTCATTTGCCGGACCTTGGAATATCTTGAAATCTACGTTTTTGACAATATCTGCCACGTCCACCAGTTCTAACGGATTGCGCAAATCCGGTTTATCGGAACCAAAACGGCGCATCGCTTCTTGCCAGGTTATCACCGGGAATTTGCCCAAATCGACACCTAAAGTATGCTGCCACAAACCGTGCACCATACGTTCCATGATTTCACGTAcc  <  1:883421/251‑1 (MQ=255)
                                                                                                                                                                                                                                          ccccTGCGGCACAAGAATTAAGGTGACACGACCATTCGGGTCATTTGCCGGACCTTGGAATATCTTGAAATCTACGTTTTTGACAATATCTGCCACGTCCACCAg                                                                                                                                                   >  1:673845/1‑105 (MQ=255)
                                                                                                                                                                                                                                                 ggCACAAGAATTAAGGTGACACGACCATTCGGGTCATTTGCCGGACCTTGGAATATCTTGAAATCTACGTTTTTGACAATATCTGCCACGTCCACCAGTTCTAACGGATTGCGCAAATCCGGTTTATCGGAACCAAAACGGCGCATCGCTTCTTGCCAGGTTATCACCGGGAATTTGCCCAAATCGACACCTAAAGTATGCTGCCACaa                                    <  1:263847/209‑1 (MQ=255)
                                                                                                                                                                                                                                                  gCACAAGAATTAAGGTGACACGACCATTCGGGTCATTTGCCGGACCTTGGAATATCTTGAAATCTACGTTTTTGACAATATCTGCCACGTCCACCAGTTCTAACGGATTGCGCAAATCCGGTTTATCGGAACCAAAACGGCGCATCGCTTCTTGCCAGGTTATCAc                                                                              >  1:532801/1‑166 (MQ=255)
                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                            
ATCTGTGGCGGTTTGCCATTTGTCGGCACCGAAGAATAAAATATCGCCAGTTTGCGCGCCCACGCGTTCAAACAAGGCTTTTAGCACGTCTTCATTTAAGAATTTTGCAATAGGGCTTTGGACGCCTTCAAGACCGGCGTTTACGTCATTCACTTTTAACCAAGCCAAGCCTTTAGCACCATAAATGCCGACAAATTGGGTATATTCATCAATTTGTTTACGGGTGATTTCATTCCCCTGCGGCACACGAATTAAGGTGACACGACCATTCGGGTCATTTGCCGGACCTTGGAATATCTTGAAATCTACGTTTTTGACAATATCTGCCACGTCCACCAGTTCTAACGGATTGCGCAAATCCGGTTTATCGGAACCAAAACGGCGCATCGCTTCTTGCCAGGTTATCACCGGGAATTTGCCCAAATCGACACCTAAAGTATGCTGCCACAAACCGTGCACCATACGTTCCATGATTTCACGTACC  >  1/2008816‑2009299

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑