Predicted mutation
evidence seq id position mutation annotation gene description
RA 1 54,600 T→G intergenic (+145/+59) unknown → / ← unknown putative lipoprotein/YpfJ protein, zinc metalloprotease superfamily

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*154,6000TG100.0% 209.5 / NA 74intergenic (+145/+59)unknown/unknownputative lipoprotein/YpfJ protein, zinc metalloprotease superfamily
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (41/33);  total (41/33)

CTAAAAATCTTCGGTAGCAAGGCAGAAGAAATCGCCGGCAAAGCTGAGATCAAAACCGGTAAGGGAACTCAAGAAATCGGTCTGGCAGGTAAAAAAGAATAAACTCGGTTTTCCTCTTGATCGGCGTAAAGGTGGTCTGAAGCTTTCAACACGGGTTTCTACCACCTTTTCTATCCATTAATCGGTTCGTAGAAAAATCCACGCTACACGCCGCAAGTTAGAATTATTACCTGACTTACGGTGGTTTACCTATTGCAGGTTTAGGAAATGATGGTAAAAACAACCGCACTTTTTTATCACATAGTTTAAAACGTATTACACTGTGCCGGATCCGCCGTATTTACACCGCGTTTAAACCATTGCAAGCGTTGCGCCGATGTGCCGTGGGTAAAGCTGTCCGGTACCACATAACCTTGGCTACGCTTTTGCAAACGATCGTCGCCCACGGATTCCGCCGCATTGAATGCTTTTTCAATAT  >  1/54354‑54831
                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                       
cTAAAAATCTTCGGTAGCAAGGCAGAAGAAATCGCCGGCAAAGCTGAGATCAAAACCGGTAAGGGAACTCAAGAAATCGGTCTGGCAGGTAAAAAAGAATAAACTCGGTTTTCCTCTTGATCGGTGTAAAGGTGGTCTGAAGCTTTCAACACGGGTTTCTACCACCTTTTCTATCCATTAATCGGTTCGTAGAAAAATCCACGCTACACGCCGCAAGTTAGAATTATTACCTGACTTACGGTGGTTGACCt                                                                                                                                                                                                                                     <  1:84232/251‑1 (MQ=255)
     aaTCTTCGGTAGCAAGGCAGAAGAAATCGCCGGCAAAGCTGAGATCAAAACCGGTAAGGGAACTCAAGAAATCGGTCTGGCAGGTAAAAAAGAATAAACTCGGTTTTCCTCTTGATCGGTGTAAAGGTGGTCTGAAGCTTTCAACACGGGTTTCTACCACCTTTTCTATCCATTAATCGGTTCGTAGAAAAATCCACGCTACACGCCGCAAGTTAGAATTATTACCTGACTTACGGTGGTTGACCTATTGc                                                                                                                                                                                                                                <  1:91014/251‑1 (MQ=255)
                 cAAGGCAGAAGAAATCGCCGGCAAAGCTGAGATCAAAACCGGTAAGGGAACTCAAGAAATCGGTCTGGCAGGTAAAAAAGAATAAACTCGGTTTTCCTCTTGATCGGTGTAAAGGTGGTCTGAAGCTTTCAACACGGGTTTCTACCACCTTTTCTATCCATTAATCGGTTCGTAGAAAAATCCACGCTACACGCCGCAAGTTAGAATTATTACCTGACTTACGGTGGTTGACCTATTGCAGGTTTAGGa                                                                                                                                                                                                                      <  1:117636/249‑1 (MQ=255)
                                          gCTGAGATCAAAACCGGTAAGGGAACTCAAGAAATCGGTCTGGCAGGTAAAAAAGAATAAACTCGGTTTTCCTCTTGATCGGTGTAAAGGTGGTCTGAAGCTTTCAACACGGGTTTCTACCACCTTTTCTATCCATTAATCGGTTCGTAGAAAAATCCACGCTACACGCCGCAAGTTAGAATTATTACCTGACTTACGGTGGTTGACCTATTGc                                                                                                                                                                                                                                <  1:15401/214‑1 (MQ=255)
                                               gaTCAAAACCGGTAAGGGAACTCAAGAAATCGGTCTGGCAGGTAAAAAAGAATAAACTCGGTTTTCCTCTTGATCGGTGTAAAGGTGGTCTGAAGCTTTCAACACGGGTTTCTACCACCTTTTCTATCCATTAATCGGTTCGTAGAAAAATCCACGCTACACGCCGCAAGTTAGAATTATTACCTGACTTACGGTGGTTGACCTATTGCAGGTTTAGGAAATGATGGTAAAAACAACCGCACTTTTTTAt                                                                                                                                                                                       >  1:318813/1‑250 (MQ=255)
                                               gaTCAAAACCGGTAAGGGAACTCAAGAAATCGGTCTGGCAGGTAAAAAAGAATAAACTCGGTTTTCCTCTTGATCGGTGTAAAGGTGGTCTGAAGCTTTCAACACGGGTTTCTACCACCTTTTCTATCCATTAATCGGTTCGTAGAAAAATCCACGCTACACGCCGCAAGTTAGAATTATTACCTGACTTACGGTGGTTGACCTATTGCAGGTTTAGGAAATGATGGTAAAAACAACCGCACTTTTTTAt                                                                                                                                                                                       >  1:89662/1‑250 (MQ=255)
                                                            aaGGGAACTCAAGAAATCGGTCTGGCAGGTAAAAAAGAATAAACTCGGTTTTCCTCTTGATCGGTGTAAAGGTGGTCTGAAGCTTTCAACACGGGTTTCTACCACCTTTTCTATCCATTAATCGGTTCGTAGAAAAATCCACGCTACACGCCGCAAGTTAGAATTATTACCTGACTTACGGTGGTTGACCTATTGCAGGTTTAGGAAATGATGGTAAAAACAACCGCACTTTTTTATCACATAGTTTaaaa                                                                                                                                                                         <  1:304777/251‑1 (MQ=255)
                                                               ggAACTCAAGAAATCGGTCTGGCAGGTAAAAAAGAATAAACTCGGTTTTCCTCTTGATCGGTGTAAAGGTGGTCTGAAGCTTTCAACACGGGTTTCTACCACCTTTTCTATCCATTAATCGGTTCGTAGAAAAATCCACGCTACACGCCGCAAGTTAGAATTATTACCTGACTTACGGTGGTTGACCTATTGCAGGTTTAGGAAATGATGGTAAAAACAACCGCACTTTTTTATCACATAGTTTAAAAc                                                                                                                                                                        <  1:138219/249‑1 (MQ=255)
                                                                      aaGAAATCGGTCTGGCAGGTAAAAAAGAATAAACTCGGTTTTCCTCTTGATCGGTGTAAAGGTGGTCTGAAGCTTTCAACACGGGTTTCTACCACCTTTTCTATCCATTAATCGGTTCGTAGAAAAATCCACGCTACACGCCGCAAGTTAGAATTATTACCTGACTTACGGTGGTTGACCTATTGCAGGTTTAGGAAATGATGGTAAAAACAACCGCACTTTTTTATCACATAGTTTAAAACGTATTacac                                                                                                                                                               <  1:832358/251‑1 (MQ=255)
                                                                      aaGAAATCGGTCTGGAAGGTAAAAAAGAATAAACGCGGTTTTCCTCTGGATCGGTGTAAAGGTGGTCTGAAGCTTTAAACACGGGTTTCTACCACCTTTTCTATCCATTAATCGGTTCGTAGAAAAATCCACGCTACACGCCGCAAGTTAGAATTATTACCTGACTTACGGTGGTTGACCTATTGCAGGTTTAGGAAATGATGGTAAAAACAACCGCACTTTTTTATCACATAGTTTAAAACGTATTacac                                                                                                                                                               <  1:45993/251‑1 (MQ=255)
                                                                                tCTGGCAGGTAAAAAAGAATAAACTCGGTTTTCCTCTTGATCGGTGTAAAGGTGGTCTGAAGCTTTCAACACGGGTTTCTACCACCTTTTCTATCCATTAATCGGTTCGTAGAAAAATCCACGCTACACGCCGCAAGTTAGAATTATTACCTGACTTACGGTGGTTGACCTATTGCAGGTTTAGGAAATGATGGTAAAAACAACCGCACTTTTTTATCACATAGTTTAAAACGTATTACACTGTGCCGGa                                                                                                                                                      <  1:840132/250‑1 (MQ=255)
                                                                                tCTGGCAGGTAAAAAAGAATAAACTCGGTTTTCCTCTTGATCGGTGTAAAGGTGGTCTGAAGCTTTCAACACGGGTTTCTACCACCTTTTCTATCCATTAATCGGTTCGTAGAAAAATCCACGCTACACGCCGCAAGTTAGAATTATTACCTGACTTACGGTGGTTGACCTATTGCAGGTTTAGGAAATGATGGTAAAAACAACCGCACTTTTTTATCACATAGTTTAAAACGTATTACACTGTGCCGGAt                                                                                                                                                     <  1:542728/251‑1 (MQ=255)
                                                                                tCTGGCAGGTAAAAAAGAATAAACTCGGTTTTCCTCTTGATCGGTGTAAAGGTGGTCTGAAGCTTTCAACACGGGTTTCTACCACCTTTTCTATCCATTAATCGGTTCGTAGAAAAATCCACGCTACACGCCGCAAGTTAGAATTATTACCTGACTTACGGTGGTTGACCTATTGCAGGTTTAGGAAATGATGGTAAAAACAACCGCACTTTTTTATCACATAGTTTAAAACGTATTACACTGTGCCGGAt                                                                                                                                                     <  1:528539/251‑1 (MQ=255)
                                                                                tCTGGCAGGTAAAAAAGAATAAACTCGGTTTTCCTCTTGATCGGTGTAAAGGTGGTCTGAAGCTTTCAACACGGGTTTCTACCACCTTTTCTATCCATTAATCGGTTCGTAGAAAAATCCACGCTACACGCCGCAAGTTAGAATTATTACCTGACTTACGGTGGTTGACCTATTGCAGGTTTAGGAAATGATGGTAAAAACAACCGCACTTTTTTATCACATAGTTTAAAACGTATTACACTGTGCCGGAt                                                                                                                                                     <  1:231137/251‑1 (MQ=255)
                                                                                tCTGGCAGGTAAAAAAGAATAAACTCGGTTTTCCTCTTGATCGGTGTAAAGGTGGTCTGAAGCTTTCAACACGGGTTTCTACCACCTTTTCTATCCATTAATCGGTTCGTAGAAAAATCCACGCTACACGCCGCAAGTTAGAATTATTACCTGACTTACGGTGGTTGACCTATTGCAGGTTTAGGAAATGATGGTAAAAACAACCGCACTTTTTTATCACATAGTTTAAAACGTATTACACTGTGCCGGAt                                                                                                                                                     <  1:372962/251‑1 (MQ=255)
                                                                                tCTGGCAGGTAAAAAAGAATAAACTCGGTTTTCCTCTTGATCGGTGTAAAGGTGGTCTGAAGCTTTCAACACGGGTTTCTACCACCTTTTCTATCCATTAATCGGTTCGTAGAAAAATCCACGCTACACGCCGCAAGTTAGAATTATTACCTGACTTACGGTGGTTGACCTATTGCAGGTTTAGGAAATGATGGTAAAAACAACCGCACTTTTTTATCACATAGTTTAAAACGTATTACACTGTGCCGGAt                                                                                                                                                     <  1:364141/251‑1 (MQ=255)
                                                                                   ggCAGGTAAAAAAGAATAAACTCGGTTTTCCTCTTGATCGGTGTAAAGGTGGTCTGAAGCTTTCAACACGGGTTTCTACCACCTTTTCTATCCATTAATCGGTTCGTAGAAAAATCCACGCTACACGCCGCAAGTTAGAATTATTACCTGACTTACGGTGGTTGACCTATTGCAGGTTTAGGAAATGATGGTAAAAACAACCGCACTTTTTTATCACATAGTTTAAAACGTATTACACTGTGCCGGATccg                                                                                                                                                  >  1:599745/1‑251 (MQ=255)
                                                                                                    aaaCTCGGTTTTCCTCTTGATCGGTGTAAAGGTGGTCTGAAGCTTTCAACACGGGTTTCTACCACCTTTTCTATCCATTAATCGGTTCGTAGAAAAATCCACGCTACACGCCGCAAGTTAGAATTATTACCTGACTTACGGTGGTTGACCTATTGCAGGTTTAGGAAATGATGGTAAAAACAACCGCACTTTTTTATc                                                                                                                                                                                      >  1:25230/1‑198 (MQ=255)
                                                                                                         cGGTTTTCCTCTTGATCGGTGTAAAGGTGGTCTGAAGCTTTCAACACGGGTTTCTACCACCTTTTCTATCCATTAATCGGTTCGTAGAAAAATCCACGCTACACGCCGCAAGTTAGAATTATTACCTGACTTACGGTGGTTGACCTATTGCAGGTTTAGGAAATGATGGTAAAAACAACCGCACTTTTTTATCACATAGTTTAAAACGTATTACACTGTGc                                                                                                                                                          >  1:116586/1‑221 (MQ=255)
                                                                                                            ttttCCTCTTGATCGGTGTAAAGGTGGTCTGAAGCTTTCAACACGGGTTTCTACCCCCTTTTCTATCCATTAATCGGTTCGTAGAAAAATCCACGCTACACGCCGCAAGTTAGAATTATTACCTGACTTACGGTGGTTGACCTATTGCAGGTTTAGGAAATGATGGTAAAAACAACCGCACTTTTTTATCACATAGTTTAAAACGTATTACACTGTGCCGGATCCGCCGTATTTACACCGCGTTTAAAcc                                                                                                                          <  1:416864/250‑1 (MQ=255)
                                                                                                                ccTCTTGATCGGTGTAAAGGTGGTCTGAAGCTTTCAACACGGGTTTCTACCACCTTTTCTATCCATTAATCGGTTCGTAGAAAAATCCACGCTACACGCCGCAAGTTAGAATTATTACCTGACTTACGGTGGTTGACCTATTGCAGGTTTAGGAAATGATGGTAAAAACAACCGCACTTTTTTATCACATAGTTTAAAACGTATTACACTGTGCCGGATCCGCCGTATTTacac                                                                                                                                      >  1:785117/1‑234 (MQ=255)
                                                                                                                ccTCTGGATCGGTGAAAAGGTGTTCTGAAGCTTTAAACACGGTTTTCTCCCACTTTTTCTATCCTTTAATCGGTTCGTAGAAAAATCCACGCTCCACGCCGCAAGTTAGAATTATTACCTGACTTACGGTGTTTGACCTATTGCAGGTTTAGGAAATGATGGTAAAAACAACCGCACTTTTTTATCACATAGTTTAAAACGTATTACACTGTGCCGGATCCGCCGTATTTACACCGCGTTTAAACCATTGc                                                                                                                     <  1:16586/251‑1 (MQ=255)
                                                                                                                   ctTGATCGGTGTAAAGGTGGTCTGAAGCTTTCAACACGGGTTTCTACCACCTTTTCTATCCATTAATCGGTTCGTAGAAAAATCCACGCTACACGCCGCAAGTTAGAATTATTACCTGACTTACGGTGGTTGACCTATTGCAGGTTTAGGAAATGATGGTAAAAACAACCGCACTTTTTTATCACATAGTTTAAAAc                                                                                                                                                                        >  1:220058/1‑197 (MQ=255)
                                                                                                                   ctTGATCGGTGTAAAGGTGGTCTGAAGCTTTCAACACGGGTTTCTACCACCTTTTCTATCCATTAATCGGTTCGTAGAAAAATCCACGCTACACGCCGCAAGTTAGAATTATTACCTGACTTACGGTGGTTGACCTATTGCAGGTTTAGGAAATGATGGTAAAAACAACCGCACTTTTTTATCACATAGTTTAAAACGTATTACACTGTGCCGGATCCGCCGTATTTACACCGCGTTTAAACCATTGCAAg                                                                                                                  >  1:573956/1‑251 (MQ=255)
                                                                                                                          ggtgTAAAGGTGGTCTGAAGCTTTCAACACGGGTTTCTACCACCTTTTCTATCCATTAATCGGTTCGTAGAAAAATCCACGCTACACGCCGCAAGTTAGAATTATTACCTGACTTACGGTGGTTGACCTATTGCAGGTTTAGGAAATGATGGTAAAAACAACCGCACTTTTTTATCACATAGTTTAAAACGTATTACACTGTGCCGGATCCGCCGTATTTACACCGCGTTTAAACCATTGCAAGCGTTgcg                                                                                                           >  1:35214/4‑251 (MQ=255)
                                                                                                                           gtgTAAAGGTGGTCTGAAGCTTTCAACACGGGTTTCTACCACCTTTTCTATCCATTAATCGGTTCGTAGAAAAATCCACGCTACACGCCGCAAGTTAGAATTATTACCTGACTTACGGTGGTTGACCTATTGCAGGTTTAGGaa                                                                                                                                                                                                                     >  1:73156/3‑144 (MQ=255)
                                                                                                                           gtgTAAAGGTGGTCTGAAGCTTTCAACACGGGTTTCTACCACCTTTTCTATCCATTAATCGGTTCGTAGAAAAATCCACGCTACACGCCGCAAGTTAGAATTATTACCTGACTTACGGTGGTTGACCTATTGCAGGTTTAGGAAATGATGGTAAAaacaac                                                                                                                                                                                                    >  1:500102/3‑161 (MQ=255)
                                                                                                                           gtgTAAAGGTGGTCTGAAGCTTTCAACACGGGTTTCTACCACCTTTTCTATCCATTAATCGGTTCGTAGAAAAATCCACGCTACACGCCGCAAGTTAGAATTATTACCTGACTTACGGTGGTTGACCTATTGCAGGTTTAGGAAATGATGGTAAAAACAACCGCACTTTTTTATc                                                                                                                                                                                      >  1:690872/3‑175 (MQ=255)
                                                                                                                           gtgTAAAGGTGGTCTGAAGCTTTCAACACGGGTTTCTACCACCTTTTCTATCCATTAATCGGTTCGTAGAAAAATCCACGCTACACGCCGCAAGTTAGAATTATTACCTGACTTACGGTGGTTGACCTATTGCAGGTTTAGGAAATGATGGTAAAAACAACCGCACTTTTTTATCACATAGTTTAAAAc                                                                                                                                                                        >  1:804864/3‑189 (MQ=255)
                                                                                                                           gtgTAAAGGTGGTCTGAAGCTTTCAACACGGGTTTCTACCACCTTTTCTATCCATTAATCGGTTCGTAGAAAAATCCACGCTACACGCCGCAAGTTAGAATTATTACCTGACTTACGGTGGTTGACCTATTGCAGGTTTAGGAAATGATGGTAAAAACAACCGCACTTTTTTATCACATAGTTTAAAAc                                                                                                                                                                        >  1:866414/3‑189 (MQ=255)
                                                                                                                           gtgTAAAGGTGGTCTGAAGCTTTCAACACGGGTTTCTACCACCTTTTCTATCCATTAATCGGTTCGTAGAAAAATCCACGCTACACGCCGCAAGTTAGAATTATTACCTGACTTACGGTGGTTGACCTATTGCAGGTTTAGGAAATGATGGTAAAAACAACCGCACTTTTTTATCACATAGTTTAAAACGTATTac                                                                                                                                                                 >  1:552903/3‑196 (MQ=255)
                                                                                                                           gtgTAAAGGTGGTCTGAAGCTTTCAACACGGGTTTCTACCACCTTTTCTATCCATTAATCGGTTCGTAGAAAAATCCACGCTACACGCCGCAAGTTAGAATTATTACCTGACTTACGGTGGTTGACCTATTGCAGGTTTAGGAAATGATGGTAAAAACAACCGCACTTTTTTATCACATAGTTTAAAACGTATTACACTGTGCCGGAt                                                                                                                                                     >  1:859049/3‑208 (MQ=255)
                                                                                                                           gtgTAAAGGTGGTCTGAAGCTTTCAACACGGGTTTCTACCACCTTTTCTATCCATTAATCGGTTCGTAGAAAAATCCACGCTACACGCCGCAAGTTAGAATTATTACCTGACTTACGGTGGTTGACCTATTGCAGGTTTAGGAAATGATGGTAAAAACAACCGCACTTTTTTATCACATAGTTTAAAACGTATTACACTGTGCCGGATCCGCCGTATTTacac                                                                                                                                      <  1:34326/221‑1 (MQ=255)
                                                                                                                           gtgTAAAGGTGGTCTGAAGCTTTCAACACGGGTTTCTACCACCTTTTCTATCCATTAATCGGTTCGTAGAAAAATCCACGCTACACGCCGCAAGTTAGAATTATTACCTGACTTACGGTGGTTGACCTATTGCAGGTTTAGGAAATGATGGTAAAAACAACCGCACTTTTTTATCACATAGTTTAAAACGTATTACACTGTGCCGGATCCGCCGTATTTACACCGCGTTTAAACCATTGCAAGCGTTgcg                                                                                                           >  1:707965/3‑250 (MQ=255)
                                                                                                                           gtgTAAAGGTGGTCTGAAGCTTTCAACACGGGTTTCTACCACCTTTTCTATCCATTAATCGGTTCGTAGAAAAATCCACGCTACACGCCGCAAGTTAGAATTATTACCTGACTTACGGTGGTTGACCTATTGCAGGTTTAGGAAATGATGGTAAAAACAACCGCACTTTTTTATCACATAGTTTAAAACGTATTACACTGTGCCGGATCCGCCGTATTTACACCGCGTTTAAACCATTGCAAGCGTTgcg                                                                                                           >  1:86580/3‑250 (MQ=255)
                                                                                                                           gtgTAAAGGTGGTCTGAAGCTTTCAACACGGGTTTCTACCACCTTTTCTATCCATTAATCGGTTCGTAGAAAAATCCACGCTACACGCCGCAAGTTAGAATTATTACCTGACTTACGGTGGTTGACCTATTGCAGGTTTAGGAAATGATGGTAAAAACAACCGCACTTTTTTATCACATAGTTTAAAACGTATTACACTGTGCCGGATCCGCCGTATTTACACCGCGTTTAAACCATTGCAAGCGTTgcg                                                                                                           >  1:239178/3‑250 (MQ=255)
                                                                                                                           gtgTAAAGGTGGTCTGAAGCTTTCAACACGGGTTTCTACCACCTTTTCTATCCATTAATCGGTTCGTAGAAAAATCCACGCTACACGCCGCAAGTTAGAATTATTACCTGACTTACGGTGGTTGACCTATTGCAGGTTTAGGAAATGATGGTAAAAACAACCGCACTTTTTTATCACATAGTTTAAAACGTATTACACTGTGCCGGATCCGCCGTATTTACACCGCGTTTAAACCATTGCAAGCGTTgcg                                                                                                           >  1:805141/3‑250 (MQ=255)
                                                                                                                                    tggtCTGAAGCTTTCAACACGGGTTTCTACCACCTTTTCTATCCATTAATCGGTTCGTAGAAAAATCCACGCTACACGCCGCAAGTTAGAATTATTACCTGACTTACGGTGGTTGACCTATTGCAGGTTTAGGAAATGATGGTAAAAACAACCGCACTTTTTTATCACATAGTTTAAAACGTATTACACTGTGCCGGATCCGCCGTATTTACACCGCGTTTAAACCATTGCAAGCGTTGCGCCGATGTGc                                                                                                  <  1:234136/250‑1 (MQ=255)
                                                                                                                                      gtCTGAAGCTTTCAACACGGGTTTCTACCACCTTTTCTATCCATTAATCGGTTCGTAGAAAAATCCACGCTACACGCCGCAAGTTAGAATTATTACCTGACTTACGGTGGTTGACCTATTGCAGGTTTAGGAAATGATGGTAAAAACAACCGCACTTTTTTATCACATAGTTTAAAACGTATTACACTGTGCCGGATCCGCCGTATTTACACCGCGTTTAAACCATTGCAAGCGTTGCGCCGATGTGCCGt                                                                                               >  1:500438/1‑251 (MQ=255)
                                                                                                                                           aaGCTTTCAACACGGGTTTCTCCCACCTTTTCTATCCATTAATCGGTTCGTAGAAAAATCCACGCTACACGCCGCAAGTTAGAATTATTACCTGACTTACGGTGGTTGACCTATTGCAGGTTTAGGAAATGATGGTAAAACCAACCGCACTTTTTTATCACATAGTTTAAAACGTATTACACTGTGCCGGATCCGCCGTATTTACACCGCGTTTAAACCATTGCAAGCGTTGCGCCGATGTGCCGTGGGt                                                                                           <  1:357408/250‑1 (MQ=255)
                                                                                                                                             gCTTTCAACACGGGTTTCTACCACCTTTTCTATCCATTAATCGGTTCGTAGAAAAATCCACGCTACACGCCGCAAGTTAGAATTATTACCTGACTTACGGTGGTTGACCTATTGCAGGTTTAGGAAATGATGGTAAAAACAACCGCACTTTTTTATCACATAGTTTAAAACGTATTacac                                                                                                                                                               >  1:883369/1‑180 (MQ=255)
                                                                                                                                               tttCAACACGGGTTTCTACCACCTTTTCTATCCATTAATCGTTTCGTAGAAAAATCCACGCTACACGCCGCAAGTTAGAATTATTACCTGACTTACGGTGGTTGACCTATTGCAGGTTTAGGAAATGATGGTAAAAACAACCGCACTTTTTTATCACATAGTTTAAAACGTATTACACTGTGCCGGATCCGCCGTATTTACACCGCGTTTAAACCATTGCAAGCGTTGCGCCGATGTGCCGTGGGTAAAGc                                                                                      <  1:224731/251‑1 (MQ=255)
                                                                                                                                                         ggTTTCTACCACCTTTTCTATCCATTAATCGGTTCGTAGAAAAATCCACGCTACACGCCGCAAGTTAGAATTATTACCTGACTTACGGTGGTTGACCTATTGCAGGTTTAGGAAATGATGGTAAAAACAACCGCACTTTTTTATCACATAGTTTAAAACGTATTACACTGTGCCGGATCCGCCGTATTTACACCGCGTTTAAACCATTGCAAGCg                                                                                                                >  1:897019/1‑215 (MQ=255)
                                                                                                                                                           tttCTACCACCTTTTCTATCCATTAATCGGTTCGTAGAAAAATCCACGCTACACGCCGCAAGTTAGAATTATTACCTGACTTACGGTGGTTGACCTATTGCAGGTTTAGGAAATGATGGTAAAAACAACCGCACTTTTTTATCACATAGTTTAAAACGTATTACACTGTGCCGGAt                                                                                                                                                     <  1:101074/176‑1 (MQ=255)
                                                                                                                                                             tCTACCACCTTTTCTATCCATTAATCGGTTCGTAGAAAAATCCACGCTACACGCCGCAAGTTAGAATTATTACCTGACTTACGGTGGTTGACCTATTGCAGGTTTAGGAAATGATGGTAAAAACAACCGCACTTTTTTATCACATAGTTTAAAACGTATTACACTGTGCCGGAt                                                                                                                                                     >  1:876429/1‑174 (MQ=255)
                                                                                                                                                                 cccccTTTTCAATCCATTAAACGTTTCGTAAAAAAATCCACGCTACACGCCGCAAGTTAGAATTATTACCTGACTTACGGTGGTTGACCTATTGCAGGTTTAGGAAATGATGGAAAAAACAACCGCACTTTTTTATCACATAGTTTAAACCGTATTACACTGTGCCGGATCCGCCGTATTTACACCGCGTTTAAACCATTGCAAGCGTTGCGCCGATGTGCCGTGGGTAAAGCTGTCCGGTACCACATagc                                                                    <  1:700516/248‑3 (MQ=255)
                                                                                                                                                                 ccaccTTTTCTATCCATTAATCGGTTCGTAGAAAAATCCACGCTACACGCCGCAAGTTAGAATTATTACCTGACTTACGGTGGTTGACCTATTGCAGGTTTAGGAAATGATGGTAAAAACAACCGCACTTTTTTATCACATAGTTTAAAACGTATTACACTGTGCCGGATCCGCCGTATTTACACCGCGTTTAAACCATTGCAAGCGTTGCGCCGATGTGCCGTGGGTAAAGCTGTCCGGTACCACATag                                                                     >  1:654524/1‑249 (MQ=255)
                                                                                                                                                                       tttCTATCCATTAATCGGTTCGTAGAAAAATCCACGCTACACGCCGCAAGTTAGAATTATTACCTGACTTACGGTGGTTGACCTATTGCAGGTTTAGGAAATGATGGTAAAAACAACCGCACTTTTTTATCACATAGTTTAAAACGTATTACACTGTGCCGGATCCGCCGTATTTACACCGCGTTTAAACCATTGCAAGCGTTGCGCCGATGTGCCGTGGGTAAAGCTGTCCGGTAc                                                                            >  1:36985/1‑237 (MQ=255)
                                                                                                                                                                        ttCTATCCATTAATCGGTTCGTAGAAAAATCCACGCTACACGCCGCAAGTTAGAATTATTACCTGACTTACGGTGGTTGACCTATTGCAGGTTTAGGAAATGATGGTAAAAACAACCGCACTTTTTTATCACATAGTTTAAAACGTATTACACTGTGCCGGATCCGCCGTATTTACACCGCGTTTAAACCATTGCAAGCGTTGCGCCGATGTGCCGTGGGTAAAGCTGTCCGGTACCACATAGCCTTGGCt                                                             <  1:154515/251‑1 (MQ=255)
                                                                                                                                                                        ttCTATCCATTAATCGGTTCGTAAAAAAACCCACGCTACACGCCGCAAGTTAGAATTATTACCTGACTTACGGTGGTTGACCTATTGCAGGTTTAGGAAATGATGGTAAAAACAACCGCACTTTTTTATCACATAGTTTAAAACGTATTACACTGTGCCGGATCCGCCGTATTTACACCGCGTTTAAACCATTGCAAGCGTTGCGCCGATGTGCCGTGGGTAAAGCTGTCCGGTACCACATAGCCTTGGCt                                                             <  1:504181/251‑1 (MQ=255)
                                                                                                                                                                         tCTATCCATTAATCGGTTCGTAGAAAAATCCACGCTACACGCCGCAAGTTAGAATTATTACCTGACTTACGGTGGTTGACCTATTGCAGGTTTAGGAAATGATGGTAAAAACAACCGCACTTTTTTATCACATAGTTTAAAACGTATTACACTGTGCCGGATCCGCCGTATTTACACCGCGTTTAAACCATTGCAAGCGTTGCGCCGATGTGCCGTGGGTAAAGCTGTCCGGTACCACATAGCCTTGGCtc                                                            <  1:696329/251‑2 (MQ=255)
                                                                                                                                                                              ccATTAATCGGTTCGTAGAAAAATCCACGCTACACGCCGCAAGTTAGAATTATTACCTGACTTACGGTGGTTGACCTATTGCAGGTTTAGGAAATGATGGTAAAAACAACCGCACTTTTTTATCACATAGTTTAAAACGTATTacac                                                                                                                                                               >  1:602515/1‑147 (MQ=255)
                                                                                                                                                                              ccATTAATCGGTTCGTAGAAAAATCCACGCTACACGCCGCAAGTTAGAATTATTACCTGACTTACGGTGGTTGACCTATTGCAGGTTTAGGAAATGATGGTAAAAACAACCGCACTTTTTTATCACATAGTTTAAAACGTATTACACTGTGCCGGAt                                                                                                                                                     <  1:760091/157‑1 (MQ=255)
                                                                                                                                                                              ccATTAATCGGTTCGTAGAAAAATCCACGCTACACGCCGCAAGTTAGAATTATTACCTGACTTACGGTGGTTGACCTATTGCAGGTTTAGGAAATGATGGTAAAAACAACCGCACTTTTTTATCACATAGTTTAAAACGTATTACACTGTGCCGGATCCGCCGTATTTACACCGCGTTTAAACCATTGCAAGCGTTGCGCCGATGTGCCGTGGGTAAAGCTGTCCGGTACCACATAGCCTTGGCTACGCtt                                                       >  1:836645/1‑251 (MQ=255)
                                                                                                                                                                              ccATTAATCGGTTCGTAGAAAAATCCACGCTACACGCCGCAAGTTAGAATTATTACCTGACTTACGGTGGTTGACCTATTGCAGGTTTAGGAAATGATGGTAAAAACAACCGCACTTTTTTATCACATAGTTTAAAACGTATTACACTGTGCCGGATCCGCCGTATTTACACCGCGTTTAAACCATTGCAAGCGTTGCGCCGATGTGCCGTGGGTAAAGCTGTCCGGTACCACATAGCCTTGGCTACGCtt                                                       >  1:181113/1‑251 (MQ=255)
                                                                                                                                                                              ccATTAATCGGTTCGTAGAAAAATCCACGCTACACGCCGCAAGTTAGAATTATTACCTGACTTACGGTGGTTGACCTATTGCAGGTTTAGGAAATGATGGTAAAAACAACCGCACTTTTTTATCACATAGTTTAAAACGTATTACACTGTGCCGGATCCGCCGTATTTACACCGCGTTTAAACCATTGCAAGCGTTGCGCCGATGTGCCGTGGGTAAAGCTGTCCGGTACCACATAGCCTTGGCTACGCtt                                                       >  1:751718/1‑251 (MQ=255)
                                                                                                                                                                               cATTAATCGGTTCGTAGAAAAATCCACGCTACACGCCGCAAGTTAGAATTATTACCTGACTTACGGTGGTTGACCTATTGCAGGTTTAGGAAATGATGGTAAAAACAACCGCACTTTTTTATCACATAGTTTAAAACGTATTACACTGTGCCGGATCCGCCGTATTTacgc                                                                                                                                      >  1:439049/1‑169 (MQ=255)
                                                                                                                                                                                aTTAATCGGTTCGTAGAAAAATCCACGCTACACGCCGCAAGTTAGAATTATTACCTGACTTACGGTGGTTGACCTATTGCAGGTTTAGGAAATGATGGTAAAAACAACCGCACTTTTTTATCACATAGTTTAAAACg                                                                                                                                                                       >  1:601183/1‑137 (MQ=255)
                                                                                                                                                                                  tAATCGTTTCGTAGAAAAATCCACGCTCCACGCCGCAAGTTAGAATTATTACCTGACTTACGGTGGTTGACCTATTGCAGGTTTAGGAAATGATGGTAAAAACAACCGCACTTTTTTATCACATAGTTTAAAACGTATTACACTGTGCCGGATCCGCCGTATTTACACCGCGTTTAAACCATTGCAAGCGTTGCGCCGATGTGCCGTGGGTAAAGCTGTCCGGTACCACATAGCCTTGGCTACGCTTTTGc                                                   <  1:813148/251‑1 (MQ=255)
                                                                                                                                                                                   aaTCGGTTCGTAGAAAAATCCACGCTACACGCCGCAAGTTAGAATTATTACCTGACTTACGGTGGTTGACCTATTGCAGGtt                                                                                                                                                                                                                           >  1:359597/1‑82 (MQ=255)
                                                                                                                                                                                             tAGAAAAATCCACGCTACACGCCGCAAGTTAGAATTATTACCTGACTTACGGTGGTTGACCTATTGCAGGTTTAGGAAATGATGGTAAAAACAACCGCACTTTTTTATCACATAGTTTAAAACGTATTACACTGTGCCGGATCCGCCGTATTTACACCGCGTTTAAACCATTGCAAGCGTTGCGCCGATGTGCCGTGGGTAAAGc                                                                                      >  1:566137/1‑205 (MQ=255)
                                                                                                                                                                                                   aaTCCACGCTACACGCCGCAAGTTAGAATTATTACCTGACTTACGGTGGTTGACCTATTGCAGGTTTAGGAAATGATGGTAAAAACAACCGCACTTTTTTATCACATAGTTTAAAACGTATTacac                                                                                                                                                               <  1:205031/126‑1 (MQ=255)
                                                                                                                                                                                                        aCGCTACACGCCGCAAGTTAGAATTATTACCTGACTTACGGTGGTTGACCTATTGCAGGTTTAGGAAATGATGGTAAAAACAACCGCACTTTTTTATCACATAGTTTAAAACGTATTACACTGTGCCGGATCCGCCGTATTTacac                                                                                                                                      >  1:643200/1‑146 (MQ=255)
                                                                                                                                                                                                        aCGCTACACGCCGCAAGTTAGAATTATTACCTGACTTACGGTGGTTGACCTATTGCAGGTTTAGGAAATGATGGTAAAAACAACCGCACTTTTTTATCACATAGTTTAAAACGTATTACACTGTGCCGGATCCGCCGTATTTACACCGCGTTTAAACCATTGCAAGCGTTGCGCCGATGTGCCGTGGGTAAAGCTGTCCGGTACCACATAGCCTTGGCTACGCTTTTGCAAACGATCGTCGCCCACGGAtt                             >  1:415524/1‑251 (MQ=255)
                                                                                                                                                                                                        aCGCTACACGCCGCAAGTTAGAATTATTACCTGACTTACGGTGGTTGACCTATTGCAGGTTTAGGAAATGATGGTAAAAACAACCGCACTTTTTTATCACATAGTTTAAAACGTATTACACTGTGCCGGATCCGCCGTATTTACACCGCGTTTAAACCATTGCAAGCGTTGCGCCGATGTGCCGTGGGTAAAGCTGTCCGGTACCACATAGCCTTGGCTACGCTTTTGCAAACGATCGTCGCCCACGGAtt                             >  1:395459/1‑251 (MQ=255)
                                                                                                                                                                                                         cGCTACACGCCGCAAGTTAGAATTATTACCTGACTTACGGTGGTTGACCTATTGCAGGTTTAGGAAATGATGGTAAAAACAACCGCACTTTTTTATCACATAGTTTAAAACGTATTACACTGTGCCGGATCCGCCGTATTTACACCGCGTTTAAACCATTGCAAGCg                                                                                                                >  1:305624/1‑167 (MQ=255)
                                                                                                                                                                                                           cTACACGCCGCAAGTTAGAATTATTACCTGACTTACGGTGGTTGACCTATTGCAGGTTTAGGAAATGATGGTAAAAACAACCGCACTTTTTTGTCACATAGTTTAAAAc                                                                                                                                                                        <  1:488983/109‑1 (MQ=255)
                                                                                                                                                                                                           cTACACGCCGCAAGTTAGAATTATTACCTGACTTACGGTGGTTGACCTATTGCAGGTTTAGGAAATGATGGTAAAAACAACCGCACTTTTTTATCACATAGTTTAAAACGTATTACACTGTGCCGGATCCGCCGTATTTACACCGCGTTTAAACCATTGCAAGCGTTGCGCCGATGTGCCGTGGGTAAAGCTGTCCGGTACCACATAGCCTTGGCTACGCTTTTGCAAACGATCGTCGCCCACGGATTccg                          >  1:398686/1‑251 (MQ=255)
                                                                                                                                                                                                                      aaGTTAGAATTATTACCTGACTTACGGTGGTTGACCTATTGCAGGTTTAGGAAATGATGGTAAAAACAACCGCACTTTTTTATCACATAGTTTAAAACGTATTACACTGTGCCGGATCCGCCGTATTTACACCGCGTTTAAACCATTGCAAGCGTTGCGCCGATGTGCCGTGGGTAAAGCTGTCCGGTACCACATAGCCTTGGCTACGCTTTTGCAAACGATCGTCGCCCACGGATTCCGCCGCATTGAAt               <  1:230126/251‑1 (MQ=255)
                                                                                                                                                                                                                         ttAGAATTATTACCTGACTTACGGTGGTTGACCTATTGCAGGTTTAGGAAATGATGGTAAAAACAACCGCACTTTTTTATCACATAGTTTAAAACGTATTacac                                                                                                                                                               <  1:192764/104‑1 (MQ=255)
                                                                                                                                                                                                                         ttAGAATTATTACCTGACTTACGGTGGTTGACCTATTGCAGGTTTAGGAAATGATGGTAAAAACAACCGCACTTTTTTATCACATAGTTTAAAACGTATTACACTGTGCCGGATCCGCCGTATTTACACCGCGTTTAAACCATTGCAAGCGTTGCGCCGATGTGCCGTGGGTAAAGCTGTCCGGTACCACATAGCCTTGGCTACGCTTTTGCAAACGATCGTCGCCCACGGATTCCGCCGCATTGAATg              >  1:541248/1‑249 (MQ=255)
                                                                                                                                                                                                                               ttattaCCTGACTTACGGTGGTTGACCTATTGCAGGTTTAGGAAATGATGGTAAAAACAACCGCACTTTTTTATCACATAGTTTAAAAc                                                                                                                                                                        <  1:470608/89‑1 (MQ=255)
                                                                                                                                                                                                                                 attaCCTGACTTACGGTGGTTGACCTATTGCAGGTTTAGGAAATGATGGTAAAAACAACCGCACTTTTTTATCACATAGTTTAAAACGTATTACACTGTGCCGGATCCGCCGTATTTACACCGCGTTTAAACCATTGCAAGCGTTGCGCCGATGTGCCGTGGGTAAAGCTGTCCGGTACCACATAGCCTTGGCTACGCTTTTGCAAACGATCGTCGCCCACGGATTCCGCCGCATTGAATGCTTTTTCAat    <  1:242647/251‑1 (MQ=255)
                                                                                                                                                                                                                                    aCCTGACTTACGGTGGTTGACCTATTGCAGGTTTAGGAAATGATGGTAAAAACAACCGCACTTTTTTATCACATAGTTTAAAACGTATTACACTGTGCCGGATCCGCCGTATTTACACCGCGTTTAAACCATTGCAAGCGTTGCGCCGATGTGCCGTGGGTAAAGCTGTCCGGTACCACATAGCCTTGGCTACGCTTTTGCAAACGATCGTCGCCCACGGATTCCGCCGCATTGAATGCTTTTTCAatat  >  1:232826/1‑250 (MQ=255)
                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                       
CTAAAAATCTTCGGTAGCAAGGCAGAAGAAATCGCCGGCAAAGCTGAGATCAAAACCGGTAAGGGAACTCAAGAAATCGGTCTGGCAGGTAAAAAAGAATAAACTCGGTTTTCCTCTTGATCGGCGTAAAGGTGGTCTGAAGCTTTCAACACGGGTTTCTACCACCTTTTCTATCCATTAATCGGTTCGTAGAAAAATCCACGCTACACGCCGCAAGTTAGAATTATTACCTGACTTACGGTGGTTTACCTATTGCAGGTTTAGGAAATGATGGTAAAAACAACCGCACTTTTTTATCACATAGTTTAAAACGTATTACACTGTGCCGGATCCGCCGTATTTACACCGCGTTTAAACCATTGCAAGCGTTGCGCCGATGTGCCGTGGGTAAAGCTGTCCGGTACCACATAACCTTGGCTACGCTTTTGCAAACGATCGTCGCCCACGGATTCCGCCGCATTGAATGCTTTTTCAATAT  >  1/54354‑54831

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑