Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,999,460	T→A	A218A (GCA→GCT)	unknown ←	Cytochrome d ubiquinol oxidase subunit II (EC 1.10.3.‑)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,999,460	0	T	A	100.0%	216.2 / NA	75	A218A (GCA→GCT)	unknown	Cytochrome d ubiquinol oxidase subunit II (EC 1.10.3.‑)
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (36/39);  total (36/39)

GGTTAATGATGAGAACAAGAAGGCAAAACCACAACGATTTGCTTTCGAGAATATTGCGTTAAGCAATGCTCCCCCTACAGCCAATACCGGGAATAACCATAAAATCGGCATTTCTTTATAGTTGTTAAACCAGGCACCGGCTTGTACTGCCACTTCTTTACCAATCGGATTGGAAGGCGCGTTGTGATCAATCACACTGGTGACAACAAAACCATCTTTGAAAGATAACCATACACCTGCCAACACAAAGGCGATTAATGTCACGGTTGCACCCACCTGAGAAATCACGCAAGCGCGTGCACGCAATTCTGACGTGGTTTTCATTTGCAGCCAGTTCGCACCATGGGTGGTTAACATTGCCAAACTGATTACACCACAAAGCAATGCAAACGGATTTAACAACCCAAAGAATGTACCGGTGTATTGCACCTGTTGTAACTCATTGAATTCAAACGGAACACCCTGTAATAAGTTACCAAATG  >  1/1999223‑1999704                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                     ggTTAATGATGAGAACAAGAAGGCAAAACCACAACGATTTGCTTTCGAGAATATTGCGTTAAGCAATGCTCCCCCTACAGCCAATACCGGGAATAACCATAAAATCGGCATTTCTTTATAGTTGTTAAACCAGGCACCGGCTTGTACTGCCACTTCTTTACCAATCGGATTGGAAGGCGCGTTGTGATCAATCACACTGGTGACAACAAAACCATCTTTGAAAGATAACCATACACCAGCCAACACAAAgg                                                                                                                                                                                                                                         >  1:360441/1‑251 (MQ=255)         atgaGAACAAGAAGGCAAAACCACAACGATTTGCTTTCGAGAATATTGCGTTAAGCAATGCTCCCCCTACAGCCAATACCGGGAATAACCATAAAATCGGCATTTCTTTATAGTTGTTAAACCAGGCACCGGCTTGTACTGCCACTTCTTTACCAATCGGATTGGAAGGCGCGTTGTGATCAATCACACTGGTGACAACAAAACCATCTTTGAAAGATAACCATACACCAGCCAACACAAAGGCGATTAAt                                                                                                                                                                                                                                 <  1:378997/251‑1 (MQ=255)                cAAGAAGGCAAAACCACAACGATTTGCTTTCGAGAATATTGCGTTAAGCAATGCTCCCCCTACAGCCAATACCGGGAATAACCATAAAATCGGCATTTCTTTATAGTTGTTAAACCAGGCACCGGCTTGTACTGCCACTTCTTTACCAATCGGATTGGAAGGCGCGTTGTGATCAATCACACTGGTGACAACAAAACCATCTTTGAAAGATAACCATACACCAGCCAACACAAAGGCGATTAATGTCACgg                                                                                                                                                                                                                          >  1:334876/1‑251 (MQ=255)                 aagaagGCAAAACCACAACGATTTGCTTTCGAGAATATTGCGTTAAGCAATGCTCCCCCTACAGCCAATACCGGGAATAACCATAAAATCGGCATTTCTTTATAGTTGTTAAACCAGGCACCGGCTTGTACTGCCACTTCTTTACCAATCGGATTGGAAGGCGCGTTGTGATCAATCACACTGGTGACAACAAAACCATCTTTGAAAGATAACCATACACCAGCCAACACAAAGGCGATTAATGTCACGGt                                                                                                                                                                                                                         <  1:714953/251‑1 (MQ=255)                      ggCAAAACCACAACGATTTGCTTTCGAGAATATTGCGTTAAGCAATGCTCCCCCTACAGCCAATACCGGGAATAACCATAAAATCGGCATTTCTTTATAGTTGTTAAACCAGGCACCGGCTTGTACTGCCACTTCTTTACCAATCGGATTGGAAGGCGCGTTGTGATCAATCACACTGGTGACAACAAAACCATCTTTGAAAGATAACCATACACCAGCCAACACAAAGGCGATTAATGTCACGGTTGca                                                                                                                                                                                                                     >  1:633417/1‑250 (MQ=255)                       gCAAAACCACAACGATTTGCTTTCGAGAATATTGCGTTAAGCAATGCTCCCCCTACAGCCAATACCGGGAATAACCATAAAATCGGCATTTCTTTATAGTTGTTAAACCAGGCACCGGCTTGTACTGCCACTTCTTTACCAATCGGATTGGAAGGCGCGTTGTGATCAATCACACTGGTGACAACAAAACCATCTTTGAAAGATAACCATACACCAGCCAACACAAAGGCGATTAATGTCACGGTTGcac                                                                                                                                                                                                                    <  1:511857/250‑1 (MQ=255)                                              cGAGAATATTGCGTTAAGCAATGCTCCCCCTACAGCCAATACCGGGAATAACCATAAAATCGGCATTTCTTTATAGTTGTTAAACCAGGCACCGGCTTGTACTGCCACTTCTTTACCAATCGGATTGGAAGGCGCGTTGTGATCAATCACACTGGTGACAACAAAACCATCTTTGAAAGATAACCATACACCAGCCAACACAAAgg                                                                                                                                                                                                                                         >  1:772734/1‑206 (MQ=255)                                                   atatTGCGTTAAGCAATGCTCCCCCTACAGCCAATACCGGGAATAACCATAAAATCGGCATTTCTTTATAGTTGTTAAACCAGGCACCGGCTTGTACTGCCACTTCTTTACCAATCGGATTGGAAGGCGCGTTGTGATCAATCACACTGGTGACAACAAAACCATCTTTGAAAGATAACCATACACCAGCCAACACAAAGGCGATTAATGTCACGGTTGCACCCACCTGAGAAATCACGCAAGCGCGTGCa                                                                                                                                                                                       >  1:849331/1‑251 (MQ=255)                                                                cAATGCTCCCCCTACAGCCAATACCGGGAATAACCATAAAATCGGCATTTCTTTATAGTTGTTAAACCAGGCACCGGCTTGTACTGCCACTTCTTTACCAATCGGATTGGAAGGCGCGTTGTGATCAATCACACTGGTGACAACAAAACCATCTTTGAAAGATAACCATACACCAGCCAACACAAAGGCGATTAATGTCACGGTTGCACCCACCTGAGAAATCACGCAAGCGCGTGCACGCAATTCTGa                                                                                                                                                                            <  1:498993/249‑1 (MQ=255)                                                                    gCTCCCCCTACAGCCAATACCGGGAATAACCATAAAATCGGCATTTCTTTATAGTTGTTAAACCAGGCACCGGCTTGTACTGCCACTTCTTTACCAATCGGATTGGAAGGCGCGTTGTGATCAATCACACTGGTGACAACAAAACCATCTTTGAAAGATAACCATACACCAGCCAACACAAAGGCGATTAATGTCACGGTTGCACCCACCTGAGAAATCACGCAAGCGCGTGCACGCAATTCTGACGTGGt                                                                                                                                                                      >  1:431070/1‑251 (MQ=255)                                                                         cccTACAGCCAATACCGGGAATAACCATAAAATCGGCATTTCTTTATAGTTGTTAAACCAGGCACCGGCTTGTACTGCCACTTCTTTACCAATCGGATTGGAAGGCGCGTTGTGATCAATCACACTGGTGACAACAAAACCATCTTTGAAAGATAACCATACACCAGCCAACACAAAGGCGATTAATGTCACGGTTGCACCCACCTGAGAAATCACGCAAGCGCGTGCACGc                                                                                                                                                                                    >  1:16273/1‑232 (MQ=255)                                                                         cccTACAGCCAATACCGGGAATAACCATAAAATCGGCATTTCTTTATAGTTGTTAAACCAGGCACCGGCTTGTACTGCCACTTCTTTACCAATCGGATTGGAAGGCGCGTTGTGATCAATCACACTGGTGACAACAAAACCATCTTTGAAAGATAACCATACACCAGCCAACACAAAGGCGATTAATGTCACGGTTGCACCCACCTGAGAAATCACGCAAGCGCGTGCACGCAATTCTGACGTGGTTTTCa                                                                                                                                                                 >  1:758123/1‑251 (MQ=255)                                                                         cccTACAGCCAATACCGGGAATAACCATAAAATCGGCATTTCTTTATAGTTGTTAAACCAGGCACCGGCTTGTACTGCCACTTCTTTACCAATCGGATTGGAAGGCGCGTTGTGATCAATCACACTGGTGACAACAAAACCATCTTTGAAAGATAACCATACACCAGCCAACACAAAGGCGATTAATGTCACGGTTGCACCCACCTGAGAAATCACGCAAGCGCGTGCACGCAATTCTGACGTGGTTTTCa                                                                                                                                                                 >  1:822480/1‑251 (MQ=255)                                                                           cTACAGCCAATACCGGGAATAACCATAAAATCGGCATTTCTTTATAGTTGTTAAACCAGGCACCGGCTTGTACTGCCACTTCTTTACCAATCGGATTGGAAGGCGCGTTGTGATCAATCACACTGGTGACAACAAAACCATCTTTGAAAGATAACCATACACCAGCCAACACAAAGGCGATTAATGTCACGGTTGCACCCACCTGAGAAATCACGCAAGCGCGTGCACGCAATTCTGACGTGGTTTTCAtt                                                                                                                                                               <  1:284045/251‑1 (MQ=255)                                                                                gCCAATACCGGGAATAACCATAAAATCGGCATTTCTTTATAGTTGTTAAACCAGGCACCGGCTTGTACTGCCACTTCTTTACCAATCGGATTGGAAGGCGCGTTGTGATCAATCACACTGGTGACAACAAAACCATCTTTGAAAGATAACCATACACCAGCCAACACAAAGGCGATTAATGTCACGGTTGCACCCACCTGAGAAATCACGCAAGCGCGTGCACGCAATTCTGACGTGGTTTTCATTTGCAg                                                                                                                                                          >  1:116892/1‑251 (MQ=255)                                                                                    aTACCGGGAATAACCATAAAATCGGCATTTCTTTATAGTTGTTAAACCAGGCACCGGCTTGTACTGCCACTTCTTTACCAATCGGATTGGAAGGCGCGTTGTGATCAATCACACTGGTGACAACAAAACCATCTTTGAAAGATAACCAAACACCAGCCAACACAAAGGCGATTAATGTCACGGTTGCACCCACCTGAGAAATCACGCAAGCGCGTGCACGCAATTCTGACGTGGTTTTCAtt                                                                                                                                                               >  1:586498/1‑242 (MQ=255)                                                                                            aaTAACCATAAAATCGGCATTTCTTTATAGTTGTTAAACCAGGCACCGGCTTGTACTGCCACTTCTTTACCAATCGGATTGGAAGGCGCGTTGTGATCAATCACACTGGTGACAACAAAACCATCTTTGAAAGATAACCATACACCAGCCAACACAAAGGCGATTAATGTCACGGTTGCACCCACCTGAGAAATCACGCAAGCGCGTGCACGCAATTCTGACGTGGTTTTCATTTGCAGCCAGTTCGCAcc                                                                                                                                              <  1:750636/251‑1 (MQ=255)                                                                                            aaTAACCATAAAATCGGCATTTCTTTATAGTTGTTAAACCAGGCACCGGCTTGTACTGCCACTTCTTTACCAATCGGATTGGAAGGCGCGTTGTGATCAATCACACTGGTGACAACAAAACCATCTTTGAAAGATAACCATACACCAGCCAACACAAAGGCGATTAATGTCACGGTTGCACCCACCTGAGAAATCACGCAAGCGCGTGCACGCAATTCTGACGTGGTTTTCATTTGCAGCCAGTTCGCAc                                                                                                                                               <  1:151473/250‑1 (MQ=255)                                                                                                 ccATAAAATCGGCATTTCTTTATAGTTGTTAAACCAGGCACCGGCTTGTACTGCCACTTCTTTACCAATCGGATTGGAAGGCGCGTTGTGATCAATCACACTGGTGACAACAAAACCATCTTTGAAAGATAACCATACACCAGCCAACACAAAGGCGATTAATGTCACGGTTGCACCCACCTGAGAAATCACGCAAGCGCGTGCACGCAATTCTGACGTGGTTTTCATTTGCAGCCAGTTCGCACCATggg                                                                                                                                         <  1:685616/251‑1 (MQ=255)                                                                                                 ccATAAAATCGGCATTTCTTTATAGTTGTTAAACCAGGCACCGGCTTGTACTGCCACTTCTTTACCAATCGGATTGGAAGGCGCGTTGTGATCAATCACACTGGTGACAACAAAACCATCTTTGAAAGATAACCATACACCAGCCAACACAAAGGCGATTAATGTCACGGTTGCACCCACCTGAGAAATCACGCAAGCGCGTGCACGCAATTCTGACGTGGTTTTCATTTGCAGCCAGTTCGCACCATggg                                                                                                                                         >  1:71303/1‑251 (MQ=255)                                                                                                 ccATAAAATCGGCATTTCTTTATAGTTGTTAAACCAGGCACCGGCTTGTACTGCCACTTCTTTACCAATCGGATTGGAAGGCGCGTTGTGATCAATCACACTGGTGACAACAAAACCATCTTTGAAAGATAACCATACACCAGCCAACACAAAGGCGATTAATGTCACGGTTGCACCCACCTGAGAAATCACGCAAGCGCGTGCACGCAATTCTGACGTGGTTTTCATTTGCAGCCAGTTCGCACCATggg                                                                                                                                         <  1:124325/251‑1 (MQ=255)                                                                                                 ccATAAAATCGGCATTTCTTTATAGTTGTTAAACCAGGCACCGGCTTGTACTGCCACTTCTTTACCAATCGGATTGGAAGGCGCGTTGTGATCAATCACACTGGTGACAACAAAACCATCTTTGAAAGATAACCATACACCAGCCAACACAAAGGCGATTAATGTCACGGTTGCACCCACCTGAGAAATCACGCAAGCGCGTGCACGCAATTCTGACGTGGTTTTCATTTGCAGCCAGTTCGCACCATggg                                                                                                                                         <  1:112107/251‑1 (MQ=255)                                                                                                 ccATAAAATCGGCATTTCTTTATAGTTGTTAAACCAGGCACCGGCTTGTACTGCCACTTCTTTACCAATCGGATTGGAAGGCGCGTTGTGATCAATCACACTGGTGACAACAAAACCATCTTTGAAAGATAACCATACACCAGCCAACACAAAGGCGATTAATGTCACGGTTGCACCCACCTGAGAAATCACGCAAGCGCGTGCACGCAATTCTGACGTGGTTTTCATTTGCAGCCAGATCGCACCATggg                                                                                                                                         >  1:613414/1‑251 (MQ=255)                                                                                                   aTAAAATCGGCATTTCTTTATAGTTGTTAAACCAGGCACCGGCTTGTACTGCCACTTCTTTACCAATCGGATTGGAAGGCGCGTTGTGATCAATCACACTGGTGACAACAAAACCATCTTTGAAAGATAACCATACACCAGCCAACACAAAGGCGATTAATGTCACGGTTGCACCCACCTGAGAAATCACGCAAGCGCGTGCACGCAATTCTGACGTGGTTTTCATTTGCAGCCAGTTCGCACCATGggtg                                                                                                                                       <  1:623220/251‑1 (MQ=255)                                                                                                         tCGGCATTTCTTTATAGTTGTTAAACCAGGCACCGGCTTGTACTGCCACTTCTTTACCAATCGGATTGGAAGGCGCGTTGTGATCAATCACACTGGTGACAACAAAACCATCTTTGAAAGATAACCATACACCAGCCAACACAAAGGCGATTAATGTCACGGTTGCACCCACCTGAGAAATCACGCAAGCGCGTGCACGCAATTCTGACGTGGTTTTCATTTGCAGCCAGTTCGCACCATGGGTGGTTAAc                                                                                                                                 <  1:679559/251‑1 (MQ=255)                                                                                                          cGGCATTTCTTTATAGTTGTTAAACCAGGCACCGGCTTGTACTGCCACTTCTTTACCAATCGGATTGGAAGGCGCGTTGTGATCAATCACACTGGTGACAACAAAACCATCTTTGAAAGATAACCATACACCAGCCAACACAAAGGCGATTAATGTCACGGTTGCACCCACCTGAGAAATCACGCAAGCGCGTGCACGCAATTCTGACGTGGTTTTCATTTGCAGCCAGTTCGCACCATGGGTGGTTAACa                                                                                                                                <  1:632093/251‑1 (MQ=255)                                                                                                                 tCTTTATAGTTGTTAAACCAGGCACCGGCTTGTACTGCCACTTCTTTACCAATCGGATTGGAAGGCGCGTTGTGATCAATCACACTGGTGACAACAAAACCATCTTTGAAAGATAACCATACACCAGCCAACACAAAGGCGATTAATGTCACGGTTGCACCCACCTGAGAAATCACGCAAGCGCGTGCACGCAATTCTGACGTGGTTTTCATTTGCAGCCAGTTCGCACCATGGGTGGTTAACAt                                                                                                                               <  1:688798/245‑1 (MQ=255)                                                                                                                   tttATAGTTGTTAAACCAGGCACCGGCTTGTACTGCCACTTCTTTACCAATCGGATTGGAAGGCGCGTTGTGATCAATCACACTGGTGACAACAAAACCATCTTTGAAAGATAACCATACACCAGCCAACACAAAGGCGATTAATGTCACGGTTGCACCCACCTGAGAAATCACGCAAGCGCGTGCACGCAATTCTGACGTGGTTTTCATTTGCAGCCAGTTCGCACCATGGGTGGTTAACATTGCCAAAc                                                                                                                       <  1:18276/251‑1 (MQ=255)                                                                                                                         gttgttAAACCAGGCACCGGCTTGTACTGCCACTTCTTTACCAATCGGATTGGAAGGCGCGTTGTGATCAATCACACTGGTGACAACAAAACCATCTTTGAAAGATAACCATACACCAGCCAACACAAAGGCGATTAATGTCACGGTTGCACCCACCTGAGAAATCACGCAAGCGCGTGCACGCAATTCTGACGTGGTTTTCATTTGCAGCCAGTTCGCACCATGGGTGGTTAACATTGCCAAACTGATTa                                                                                                                 >  1:751924/1‑251 (MQ=255)                                                                                                                          ttgttAAACCAGGCACCGGCTTGTACTGCCACTTCTTTACCAATCGGATTGGAAGGCGCGTTGTGATCAATCACACTGGTGACAACAAAACCATCTTTGAAAGATAACCATACACCAGCCAACACAAAGGCGATTAATGTCACGGTTGCACCCACCTgaga                                                                                                                                                                                                          <  1:756714/161‑1 (MQ=255)                                                                                                                          ttgttAAACCAGGCACCGGCTTGTACTGCCACTTCTTTACCAATCGGATTGGAAGGCGCGTTGTGATCAATCACACTGGTGACAACAAAACCATCTTTGAAAGATAACCATACACCAGCCAACACAAAGGCGATTAATGTCACGGTTGCACCCACCTGAGAAATCACGCAAGCGCGTGCACGCAATTCTGACGTGGTTTTCATTTGCAGCCAGTTCGCACCATGGGTGGTTAACATTGCCAAACTGATTac                                                                                                                <  1:824494/251‑1 (MQ=255)                                                                                                                             ttAAACCAGGCACCGGCTTGTACTGCCACTTCTTTACCAATCGGATTGGAAGGCGCGTTGTGATCAATCACACTGGTGACAACAAAACCATCTTTGAAAGATAACCATACACCAGCCAACACAAAGGCGATTAATGTCACGGTTGCACCCACCTGAGAAATCACGCAAGCGCGTGCACGCAATTCTGACGTGGTTTTCATTTGCAGCCAGTTCGCACCATGGGTGGTTAACATTGCCAAACTGATTAcacc                                                                                                             <  1:340833/251‑1 (MQ=255)                                                                                                                             ttAAACCAGGCACCGGCTTGTACTGCCACTTCTTTACCAATCGGATTGGAAGGCGCGTTGTGATCAATCACACTGGTGACAACAAAACCATCTTTGAAAGATAACCATACACCAGCCAACACAAAGGCGATTAATGTCACGGTTGCACCCACCTGAGAAATCACGCAAGCGCGTGCACGCAATTCTGACGTGGTTTTCATTTGCAGCCAGTTCGCACCATGGGTGGTTAACATTGCCAAACTGATTAcacc                                                                                                             <  1:386419/251‑1 (MQ=255)                                                                                                                               aaaCCAGGCACCGGCTTGTACTGCCACTTCTTTACCAATCGGATTGGAAGGCGCGTTGTGATCAATCACACTGGTGACAACAAAACCATCTTTGAAAGATAACCATACACCAGCCAACACAAAGGCGATTAATGTCACGGTTGCACCCACCTGAGAAATCACGCAAGCGCGTGCACGCAATTCTGACGTGGTTTTCATTTGCAGCCAGTTCGCACCATGGGTGGTTAACATTGCCAAACTGATTAcaccac                                                                                                           <  1:398062/251‑1 (MQ=255)                                                                                                                               aaaCCAGGCACCGGCTTGTACTGCCACTTCTTTACCAATCGGATTGGAAGGCGCGTTGTGATCAATCACACTGGTGACAACAAAACCATCTTTGAAAGATAACCATACACCAGCCAACACAAAGGCGATTAATGTCACGGTTGCACCCACCTGAGAAATCACGCAAGCGCGTGCACGCAATTCTGACGTGGTTTTCATTTGCAGCCAGTTCGCACCATGGGTGGTTAACATTGCCAAACTGATTAcacca                                                                                                            >  1:28621/1‑250 (MQ=255)                                                                                                                                 aCCAGGCACCGGCTTGTACTGCCACTTCTTTACCAATCGGATTGGAAGGCGCGTTGTGATCAATCACACTGGTGACAACAAAACCATCTTTGAAAGATAACCATACACCAGCCAACACAAAGGCGATTAATGTCACGGTTGCACCCACCTGAGAAATCACGCAAGCGCGTGCACGCAATTCTGACGTGGTTTTCATTTGCAGCCAGTTCGCACCATGGGTGGTTAACAt                                                                                                                               <  1:684613/229‑1 (MQ=255)                                                                                                                                    aGGCACCGGCTTGTACTGCCACTTCTTTACCAATCGGATTGGAAGGCGCGTTGTGATCAATCACACTGGTGACAACAAAACCATCTTTGAAAGATAACCATACACCAGCCAACACAAAGGCGATTAATGTCACGGTTGCACCCACCTGAGAAATCACGCAAGCGCGTGCACGCAATTCTGACGTGGTTTTCATTTGCAGCCAGTTCGCACCATGGGTGGTTAACATTGCCAAACTGATTACACCACAAAg                                                                                                       >  1:477480/1‑250 (MQ=255)                                                                                                                                     ggCACCGGCTTGTACTGCCACTTCTTTACCAATCGGATTGGAAGGCGCGTTGTGATCAATCACACTGGTGACAACAAAACCATCTTTGAAAGATAACCATACACCAGCCAACACAAAGGCGATTAATGTCACGGTTGcaccc                                                                                                                                                                                                                  <  1:859642/142‑1 (MQ=255)                                                                                                                                      gCACCGGCTTGTACTGCCACTTCTTTACCAATCGGATTGGAAGGCGCGTTGTGATCAATCACACTGGTGACAACAAAACCATCTTTGAAAGATAACCATACACCAGCCAACACAAAGGCGATTAATGTCACGGTTGCACCCACCTGAGAAATCACGCAAGCGCGTGCACGCAATTCTGACGTGGTTTTCATTTGCAGCCAGTTCGCACCATGGGTGGTTAACATTGCCAAACTGATTACACCACAAAGc                                                                                                      <  1:210851/249‑1 (MQ=255)                                                                                                                                       cACCGGCTTGTACTGCCACTTCTTTACCAATCGGATTGGAAGGCGCGTTGTGATCAATCACACTGGTGACAACAAAACCATCTTTGAAAGATAACCATACACCAGCCAACACAAAGGCGATTAATGTCACGGTTGCACCCACCTGAGAAATCACGCAAGCGCGTGCACGCAATTCTGACGTGGTTTTCATTTGCAGCCAGTTCGCACCATGGGTGGTTAACATTGCCAAACTGATTACACCACAAAGCaa                                                                                                    <  1:108878/250‑1 (MQ=255)                                                                                                                                           ggCTTGTACTGCCACTTCTTTACCAATCGGATTGGAAGGCGCGTTGTGATCAATCACACTGGTGACAACAAAACCATCTTTGAAAGATAACCATACACCAGCCAACACAAAGGCGATTAATGTCACGGTTGCACCCACCTGAGAAATCACGCAAGCGCGTGCACGCAATTCTGACGTGGTTTTCATTTGCAGCCAGTTCGCACCATGGGTGGTTAACATTGCCAAACTGATTACACCACAAAGc                                                                                                      >  1:328182/1‑244 (MQ=255)                                                                                                                                           ggCTTGTACTGCCACTTCTTTACCAATCGGATTGGAAGGCGCGTTGTGATCAATCACACTGGTGACAACAAAACCATCTTTGAAAGATAACCATACACCAGCCAACACAAAGGCGATTAATGTCACGGTTGCACCCACCTGAGAAATCACGCAAGCGCGTGCACGCAATTCTGACGTGGTTTTCATTTGCAGCCAGTTCGCACCATGGGTGGTTAACATTGCCAAACTGATTACACCACAAAGCAATGCaa                                                                                               >  1:367992/1‑251 (MQ=255)                                                                                                                                                gTACTGCCACTTCTTTACCAATCGGATTGGAAGGCGCGTTGTGATCAATCACACTGGTGACAACAAAACCATCTTTGAAAGATAACCATACACCAGCCAACACAAAGGCGATTAATGTCACGGTTGCACCCACCTGAGAAATCACGCAAGCGCGTGCACGCAATTCTGACGTGGTTTTCATTTGCAGCCAGTTCGCACCATGGGTGGTTAACATTGCCAAACTGATTACACCACAAAGCAATGCAAACGGa                                                                                          >  1:603762/1‑251 (MQ=255)                                                                                                                                                gTACTGCCACTTCTTTACCAATCGGATTGGAAGGCGCGTTGTGATCAATCACACTGGTGACAACAAAACCATCTTTGAAAGATAACCATACACCAGCCAACACAAAGGCGATTAATGTCACGGTTGCACCCACCTGAGAAATCACGCAAGCGCGTGCACGCAATTCTGACGTGGTTTTCATTTGCAGCCAGTTCGCACCATGGGTGGTTAACATTGCCAAACTGATTACACCACAAAGCAATGCAAACGGa                                                                                          >  1:862896/1‑251 (MQ=255)                                                                                                                                                     gCCACTTCTTTACCAATCGGATTGGAAGGCGCGTTGTGATCAATCACACTGGTGACAACAAAACCATCTTTGAAAGATAACCATACACCAGCCAACACAAAGGCGATTAATGTCACGGTTGCACCCACCTGAGAAATCACGCAAGCGCGTGCACGCAATTCTGa                                                                                                                                                                            >  1:589366/1‑164 (MQ=255)                                                                                                                                                     gCCACTTCTTTACCAATCGGATTGGAAGGCGCGTTGTGATCAATCACACTGGTGACAACAAAACCATCTTTGAAAGATAACCATACACCAGCCAACACAAAGGCGATTAATGTCACGGTTGCACCCACCTGAGAAATCACGCAAGCGCGTGCACGCAATTCTGACGTGGTTTTCATTTGCAGCCAGTTCGCACCATGGGTGGTTAACATTGCCAAACTGATTACACCACAAAGCAATGCAAACGGATTTaa                                                                                     >  1:877837/1‑251 (MQ=255)                                                                                                                                                       cACTTCTTTACCAATCGGATTGGAAGGCGCGTTGTGATCAATCACACTGGTGACAACAAAACCATCTTTGAAAGATAACCATACACCAGCCAACACAAAGGCGATTAATGTCACGGTTGCACCCACCTGAGAAATCACGCAAGCGCGTGCACGCAATTCTGACGTGGTTTTCATTTGCAGCCAGTTCGCACCATGGGTGGTTAACATTGCCAAACTGATTACACCACAAAGCAATGCAAACGGATTTaaca                                                                                   <  1:516174/251‑1 (MQ=255)                                                                                                                                                         cttcttTACCAATCGGATTGGAAGGCGCGTTGTGATCAATCACACTGGTGACAACAAAACCATCTTTGAAAGATAACCATACACCAGCCAACACAAAGGCGATTAATGTCACGGTTGCACCCACCTGAGAAATCACGCAAGCGCGTGCACGCAATTCTGACGTGGTTTTCATTTGCAGCCAGTTCGCACCATGGGTGGTTAACATTGCCAAACTGATTACACCACAAAGCAATGCAAACgg                                                                                           <  1:417382/241‑1 (MQ=255)                                                                                                                                                           tcttTACCAATCGGATTGGAAGGCGCGTTGTGATCAATCACACTGGTGACAACAAAACCATCTTTGAAAGATAACCATACACCAGCCAACACAAAGGCGATTAATGTCACGGTTGCACCCACCTGAGAAATCACGCAAGCGCGTGCACGCAATTCTGACGTGGTTTTCATTTGCAGCCAGTTCGCACCATGGGTGGTTAACATTGCCAAACTGATTACACCACAAAGCAATGCAAACGGATTTAACAAcc                                                                                >  1:320888/1‑250 (MQ=255)                                                                                                                                                                aCCAATCGGATTGGAAGGCGCGTTGTGATCAATCACACTGGTGACAACAAAACCATCTTTGAAAGATAACCATACACCAGCCAACACAAAGGCGATTAATGTCACGGTTGCACCCACCTGAGAAATCACGCAAGCGCGTGCACGCAATTCTGACGTGGTTTTCATTTGCAGCCAGTTCGCACCATGGGTGGTTAACATTGCCAAACTGATTACACCACAAAGCAATGCAAACGGATTTAACAACCCAAAGa                                                                          <  1:494926/251‑1 (MQ=255)                                                                                                                                                                    aTCGGATTGGAAGGCGCGTTGTGATCAATCACACTGGTGACAACAAAACCATCTTTGAAAGATAACCATACACCAGCCAACACAAAGGCGATTAATGTCACGGTTGCACCCACCTGAGAAATCACGCAAGCGCGTGCACGCAATTCTGACGTGGTTTTCATTTGCAGCCAGTTCGCACCATGGGTGGTTAACATTGCCAAACTGATTACACCACAAAGCAATGCAAACGGATTTAACAACCCAAAGAATGt                                                                      <  1:65434/251‑1 (MQ=255)                                                                                                                                                                        gATTGGAAGGCGCGTTGTGATCAATCACACTGGTGACAACAAAACCATCTTTGAAAGATAACCATACACCAGCCAACACAAAGGCGATTAATGTCACGGTTGCACCCACCTGAGAAATCACGCAAGCGCGTGCACGCAATTCTGACGTGGTTTTCATTTGCAGCCAGTTCGCACCATGGGTGGTTAACATTGCCAAACTGATTACACCACAAAGCAATGCAAACGGATTTAACAACCCAAAGAATGTACCg                                                                  >  1:7963/1‑251 (MQ=255)                                                                                                                                                                            ggAAGGCGCGTTGTGATCAATCACACTGGTGACAACAAAACCATCTTTGAAAGATAACCATACACCAGCCAACACAAAGGCGATTAATGTCACGGTTGCACCCACCTGAGAAATCACGCAAGCGCGTGCACGCAATTCTGACGTGGTTTTCATTTGCAGCCAGTTCGCACCATGGGTGGTTAACATTGCCAAACTGATTACACCACAAAGc                                                                                                      <  1:781373/211‑1 (MQ=255)                                                                                                                                                                                  cgcgTTGTGATCAATCACACTGGTGACAACAAAACCATCTTTGAAAGATAACCATACACCAGCCAACACAAAGGCGATTAATGTCACGGTTGCACCCACCTGAGAAATCACGCAAGCGCGTGCACGCAATTCTGACGTGGTTTTCATTTGCAGCCAGTTCGCACCATGGGTGGTTAACATTGCCAAACTGATTACACCACAAAGCAATGCAAACGGATTTAACAACCCAAAGAATGTACCGGTGTATTgt                                                         <  1:888597/250‑2 (MQ=255)                                                                                                                                                                                     gTTGTGATCAATCACACTGGTGACAACAAAACCATCTTTGAAAGATAACCATACACCAGCCAACACAAAGGCGATTAATGTCACGGTTGCACCCACCTGAGAAATCACGCAAGCGCGTGCACGCAATTCTGACGTGGTTTTCATTTGCAGCCAGTTCGCACCATGGGTGGTTAACAt                                                                                                                               >  1:75593/1‑177 (MQ=255)                                                                                                                                                                                     gTTGTGATCAATCACACTGGTGACAACAAAACCATCTTTGAAAGATAACCATACACCAGCCAACACAAAGGCGATTAATGTCACGGTTGCACCCACCTGAGAAATCACGCAAGCGCGTGCACGCAATTCTGACGTGGTTTTCATTTGCAGCCAGTTCGCACCATGGGTGGTTAACATTGCCAAACTGATTACACCACAAAGCAATGCAAACGGATTTAACAACCCAAAGAATGTACCGGTGTATTgtac                                                       >  1:495043/1‑246 (MQ=255)                                                                                                                                                                                        gtgATCAATCACACTGGTGACAACAAAACCATCTTTGAAAGATAACCATACACCAGCCAACACAAAGGCGATTAATGTCACGGTTGCACCCACCTGAGAAATCACGCAAGCGCGTGCACGCAATTCTGACGTGGTTTTCATTTGCAGCCAGTTCGCACCATGGGTGGTTAACATTGCCAAACTGATTACACCACAAAGCAATGCACACGGATTTAACAACCCAAAGAATGTACCGGTGTATTGTACCtgtt                                                  >  1:365088/1‑251 (MQ=255)                                                                                                                                                                                        gtgATCAATCACACTGGTGACAACAAAACCATCTTTGAAAGATAACCATACACCAGCCAACACAAAGGCGATTAATGTCACGGTTGCACCCACCTGAGAAATCACGCAAGCGCGTGCACGCAATTCTGACGTGGTTTTCATTTGCAGCCAGTTCGCACCATGGGTGGTTAACATTGCCAAACTGATTACACCACAAAGCAATGCAAACGGATTTAACAACCCAAAGAATGTACCGGTGTATTGTACCtgtt                                                  >  1:888863/1‑251 (MQ=255)                                                                                                                                                                                         tgATCAATCACACTGGTGACAACAAAACCATCTTTGAAAGATAACCATACACCAGCCAACACAAAGGCGATTAATGTCACGGTTGCACCCACCTGAGAAATCACGCAAGCGCGTGCACGCAATTCTGACGTGGTTTTCATTTGCAGCCAGTTCGCACCATGGGTGGTTAACATTGCCAAACTGATTACACCACAAAGCAATGCAAACGGATTTAACAACCCAAAGAATGTACCGGTGTATTGTACCtgttg                                                 <  1:48476/251‑1 (MQ=255)                                                                                                                                                                                           atcaatcaCACTGGTGACAACAAAACCATCTTTGAAAGATAACCATACACCAGCCAACACAAAGGCGATTAATGTCACGGTTGCACCCACCTGAGAAATCACGCAAGCGCGTGCACGCAATTCTGACGTGGTTTTCATTTGCAGCCAGTTCGCACCATGGGTGGTTAACATTGCCAAACTGATTACACCACAAAGCAATGCAAACGGATTTAACAAccc                                                                               >  1:856946/1‑219 (MQ=255)                                                                                                                                                                                             caatcaCACTGGTGACAACAAAACCATCTTTGAAAGATAACCATACACCAGCCAACACAAAGGCGATTAATGTCACGGTTGCACCCACCTGAGAAATCACGCAAGCGCGTGCACGCAATTCTGACGTGGTTTTCATTTGCAGCCAGTTCGCACCATGGGTGGTTAACATTGCCAAACTGATTACACCACAAAGCAATGCAAACGGATTTAACAACCCAAAGAATGTACCGGTGTATTGTACCTGTTGTaa                                              >  1:60262/1‑250 (MQ=255)                                                                                                                                                                                              aatcaCACTGGTGACAACAAAACCATCTTTGAAAGATAACCATACACCAGCCAACACAAAGGCGATTAATGTCACGGTTGCACCCACCTGAGAAATCACGCAAGCGCGTGCACGCAATTCTGACGTGGTTTTCATTTGCAGCCAGTTCGCACCATGGGTGGTTAACATTGCCAAACTGATTACACCACAAAGCAATGCAAACGGATTTAACAACCCAAAGAATGTACCGGTGTATTGTACCTGTTGTaa                                              >  1:630516/1‑249 (MQ=255)                                                                                                                                                                                              aatcaCACTGGTGACAACAAAACCATCTTTGAAAGATAACCATACACCAGCCAACACAAAGGCGATTAATGTCACGGTTGCACCCACCTGAGAAATCACGCAAGCGCGTGCACGCAATTCTGACGTGGTTTTCATTTGCAGCCAGTTCGCACCATGGGTGGTTAACATTGCCAAACTGATTACACCACAAAGCAATGCAAACGGATTTAACAACCCAAAGAATGTACCGGTGTATTGTACCTGTTGTAACt                                            <  1:830393/251‑1 (MQ=255)                                                                                                                                                                                               atcaCACTGGTGACAACAAAACCATCTTTGAAAGATAACCATACACCAGCCAACACAAAGGCGATTAATGTCACGGTTGCATCCACCTGAGAAATCACGCAAGCGCGTGCACGCAATTCTGACGTGGTTTTCATTTGCAGCCAGTTCGCACCATGGGTGGTTAACATTGCCAAACTGATTACACCACAAAGCAATGCAAACGGATTTAACAACCCAAAGAATGTACCGGTGTATTGTACCTGTTGTAACTc                                           <  1:449501/251‑1 (MQ=255)                                                                                                                                                                                                tcaCACTGGTGACAACAAAACCATCTTTGAAAGATAACCATACACCAGCCAACACAAAGGCGATTAATGTCACGGTTGCACCCACCTGAGAAATCACGCAAGCGCGTGCACGCAATTCTGACGTGGTTTTCATTTGCAGCCAGTTCGCACCATGGGTGGTTAACATTGCCAAACTGATTACACCACAAAGCAATGCAAACGGATTTAACAACCCAAAGaa                                                                         >  1:869829/1‑220 (MQ=255)                                                                                                                                                                                                                  aaCCATCTTTGAAAGATAACCATACACCAGCCAACACAAAGGCGATTAATGTCACGGTTGCACCCACCTGAGAAATCACGCAAGCGCGTGCACGCAATTCTGACg                                                                                                                                                                          <  1:450387/105‑1 (MQ=255)                                                                                                                                                                                                                         tttGAAAGATAACCATACACCAGCCAACACAAAGGCGATTAATGTCACGGTTGCACCCACCTGAGAAATCACGCAAGCGCGTGCACGCAATTCTGACGTGGTTTTCATTTGCAGCCAGTTCGCACCATGGGTGGTTAACATTGCCAAACTGATTACACCACAAAGCAATGCAAACGGATTTAACAACCCAAAGAATGTACCGgtg                                                               >  1:857587/1‑205 (MQ=255)                                                                                                                                                                                                                           tGAAAGATAACCATACACCAGCCAACACAAAGGCGATTAATGTCACGGTTGCACCCACCTGAGAAATCACGCAAGCGCGTGCACGCAATTCTGACGTGGTTTTCATTTGCAGCCAGTTCGCACCATGGGTGGTTAACATTGCCAAACTGATTACACCACAAAGCAATGCAAACGGATTTAACAACCCAAAGAATGTACCGGTGTATTGTACCTGTTGTAACTCATTGAATTCAAACGGAACACCCTGtaat               <  1:241881/251‑1 (MQ=255)                                                                                                                                                                                                                           tGAAAGATAACCATACACCAGCCAACACAAAGGCGATTAATGTCACGGTTGCACCCACCTGAGAAATCACGCAAGCGCGTGCACGCAATGCTGACGTGGTTTTCATTTGCAGCCAGTTCGCACCATGGGTGGTTAACATTGCCAAACTGATTACACCACAAAGCAATGCAAACGGATTTAACAACCCAAAGAATGTACCGGTGTATTGTACCTGTTGTAACTCATTGAATTCAAACGGAACACCCTGtaat               <  1:222651/251‑1 (MQ=255)                                                                                                                                                                                                                              aaGATAACCATACACCAGCCAACACAAAGGCGATTAATGTCACGGTTGCACCCACCTGAGAAATCACGCAAGCGCGTGCACGCAATTCTGACGTGGTTTTCATTTGCAGCCAGTTCGCACCATGGGTGGTTAACATTGCCAAACTGATTACACCACAAAGCAATGCAAACGGATTTAACAACCCAAAGAATGTACCGGTGTATTGTACCTGTTGTAACTCATTGAATTCAAACGGAACACCCTGTAATAAg            <  1:200686/251‑1 (MQ=255)                                                                                                                                                                                                                               aGATAACCATACACCAGCCAACACAAAGGCGATTAATGTCACGGTTGCACCCACCTGAGAAATCACGCAAGCGCGTGCACGCAATTCTGACGTGGTTTTCATTTGCAGCCAGTTCGCACCATGGGTGGTTAAc                                                                                                                                 <  1:520128/133‑1 (MQ=255)                                                                                                                                                                                                                                 aTAACCATACACCAGCCAACACAAAGGCGATTAATGTCACGGTTGCACCCACCTGAGAAATCACGCAAGCGCGTGCACGCAATTCTGACGTGGTTTTCATTTGCAGCCAGTTCGCACCATGGGTGGTTAACATTGCCAAACTGATTACACCACAAAGCAATGCAAACGGATTTAACAACCCAAAGAATGTACCGGTGTATTGTACCTGTTGTAACTCATTGAATTCAAACGGAACACCCTGTAATAAGt           <  1:39920/249‑1 (MQ=255)                                                                                                                                                                                                                                    aCCATACACCAGCCAACACAAAGGCGATTAATGTCACGGTTGCACCCACCTGAGAAATCACGCAAGCGCGTGCACGCAATTCTGACGTGGTTTTCATTTGCAGCCAGTTCGCACCATGGGTGGTTAACATTGCCAAACTGATTACACCACAAAGCAATGCAAACGGATTTAACAACCCAAAGAATGTACCGGTGTATTGTACCTGTTGTAACTCATTGAATTCAAACGGAACACCCTGTAATAAGt           >  1:607144/1‑246 (MQ=255)                                                                                                                                                                                                                                    aCCATACACCAGCCAACACAAAGGCGATTAATGTCACGGTTGCACCCACCTGAGAAATCACGCAAGCGCGTGCACGCAATTCTGACGTGGTTTTCATTTGCAGCCAGTTCGCACCATGGGTGGTTAACATTGCCAAACTGATTACACCACAAAGCAATGCAAACGGATTTAACAACCCAAAGAATGTACCGGTGTATTGTACCTGTTGTAACTCATTGAATTCAAACGGAACACCCTGTAATAAGTTACCa      >  1:604594/1‑251 (MQ=255)                                                                                                                                                                                                                                         acacCAGCCAACACAAAGGCGATTAATGTCACGGTTGCACCCACCTGAGAAATCACGCAAGCGCGTGCACGCAATTCTGACGTGGTTTTCATTTGCAGCCAGTTCGCACCATGGGTGGTTAACATTGCCAAACTGATTACACCACAAAGCAATGCAAACGGATTTAACAACCCAAAGAATGTACCGGTGTATTGTACCTGTTGTAACTCATTGAATTCAAACGGAACACCCTGTAATAAGTTACCAAATg  >  1:784382/1‑250 (MQ=255)                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                     GGTTAATGATGAGAACAAGAAGGCAAAACCACAACGATTTGCTTTCGAGAATATTGCGTTAAGCAATGCTCCCCCTACAGCCAATACCGGGAATAACCATAAAATCGGCATTTCTTTATAGTTGTTAAACCAGGCACCGGCTTGTACTGCCACTTCTTTACCAATCGGATTGGAAGGCGCGTTGTGATCAATCACACTGGTGACAACAAAACCATCTTTGAAAGATAACCATACACCTGCCAACACAAAGGCGATTAATGTCACGGTTGCACCCACCTGAGAAATCACGCAAGCGCGTGCACGCAATTCTGACGTGGTTTTCATTTGCAGCCAGTTCGCACCATGGGTGGTTAACATTGCCAAACTGATTACACCACAAAGCAATGCAAACGGATTTAACAACCCAAAGAATGTACCGGTGTATTGCACCTGTTGTAACTCATTGAATTCAAACGGAACACCCTGTAATAAGTTACCAAATG  >  1/1999223‑1999704

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑