Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,996,401	G→A	A140A (GCC→GCT)	unknown ←	TolA protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,996,401	0	G	A	98.8%	240.4 / ‑5.2	84	A140A (GCC→GCT)	unknown	TolA protein
Reads supporting (aligned to +/- strand):  ref base G (1/0);  new base A (32/51);  total (33/51)
Fisher's exact test for biased strand distribution p-value = 3.93e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.72e-01

GCTTCCGCCTTCGCTTTAGCCTGCGCCTCTGCTTTTGCCTTAGCTTCCGCCTCTGCTTTGGCTTTTGCCTCTTGTTTTGCTTTCTCGTCTGCCTGCACTTTGGCTTTTGCTTCTTCTTCCGCTTGTTTTGCAGCATCTGCTAAACGTTTTGCCTCAGCTTCAGCTTTGAGCTTAGCGGCTTCAGCAGCCTGTTTGGCTTTCGCCTCTTCTGCCTGTTTTTGCTTTTCCAAGGCTTCTAAGCGGGCTTTTTCTTCCTGTTGCTTTTTCTGTTCAGCCAAGAACCTTTGTCTTTCCAGCTCTTTTTGCTGTTCCAGTTCTTTCTGACGGGCAATTTCCTGTTGACGTTGTTGCTCTTTTAACACTTCCCGTTGTTTTTCTTCTTCCCGTTTCTGCTCTTCACGTTTTTGGTCTTCAAGGGCTTGTTTCTTTTGTCTGTCCGCCTGACCTTTTTTCTGTTGTTGAATTCGCCCCCATTCCTGCGCTGCCG  >  1/1996159‑1996645                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                     gCTTCCGCCTTCGCTTTAGCCTGCGCCTCTGCTTTTGCCTTAGCTTCCGCCTCTGCTTTGGCTTTTGCCTCTTGTTTTGCTTTCTCGTCTGCCTGCACTTTGGCTTTTGCTTCTTCTTCCGCTTGTTTTGCAGCATCTGCTAAACGTTTTGCCTCAGCTTCAGCTTTGAGCTTAGCGGCTTCAGCAGCCTGTTTGGCTTTCGCCTCTTCTGCCTGTTTTTGCTTTTCCAAGGCTTCTAAGCGAGCttttt                                                                                                                                                                                                                                               >  1:268724/1‑250 (MQ=255)    tCCGCCTTCGCTTTAGCCTGCGCCTCTGCTTTTGCCTTAGCTTCCGCCTCTGCTTTGGCTTTTGCCTCTTGTTTTGCTTTCTCGTCTGCCTGCACTTTGGCTTTTGCTTCTTCTTCCGCTTGTTTTGCAGCATCTGCTAAACGTTTTGCCTCAGCTTCAGCTTTGAGCTTAGCGGCTTCAGCAGCCTGTTTGGCTTTCGCCTCTTCTGCCTGTTTTTGCTTTTCCAAGGCTTCTAAGCGAGCTTTttcttc                                                                                                                                                                                                                                           <  1:468185/251‑1 (MQ=255)     ccGCCTTCGCTTTAGCCTGCGCCTCTGCTTTTGCCTTAGCTTCCGCCTCTGCTTTGGCTTTTGCCTCTTGTTTTGCTTTCTCGTCTGCCTGCACTTTGGCTTTTGCTTCTTCTTCCGCTTGTTTTGCAGCATCTGCTAAACGTTTTGCCTCAGCTTCAGCTTTGAGCTTAGCGGCTTCAGCAGCCTGTTTGGCTTTCGCCTCTTCTGCCTGTTTTTGCTTTTCCAAGGCTTCTAAGCGAGCTTTTTCTTcc                                                                                                                                                                                                                                          <  1:525162/251‑1 (MQ=255)                  aGCCTGCGCCTCTGCTTTTGCCTTAGCTTCCGCCTCTGCTTTGGCTTTTGCCTCTTGTTTTGCTTTCTCGTCTGCCTGCACTTTGGCTTTTGCTTCTTCTTCCGCTTGTTTTGCAGCATCTGCTAAACGTTTTGCCTCAGCTTCAGCTTTGAGCTTAGCGGCTTCAGCAGCCTGTTTGGCTTTCGCCTCTTCTGCCTGTTTTTGCTTTTCCAAGGCTTCTAAGCGAGCTTTTTCTTCCTGTTGCttttt                                                                                                                                                                                                                               >  1:683786/1‑249 (MQ=255)                          ccTCTGCTTTTGCCTTAGCTTCCGCCTCTGCTTTGGCTTTTGCCTCTTGTTTTGCTTTCTCGTCTGCCTGCACTTTGGCTTTTGCTTCTTCTTCCGCTTGTTTTGCAGCATCTGCTAAACGTTTTGCCTCAGCTTCAGCTTTGAGCTTAGCGGCTTCAGCAGCCTGTTTGGCTTTCGCCTCTTCTGCCTGTTTTTGCTTTTCCAAGGCTTCTAAGCGAGCTTTTTCTTCCTGTTGCTTTTTCTGTTCAGc                                                                                                                                                                                                                      >  1:197769/1‑250 (MQ=255)                          ccTCTGCTTTTGCCTTAGCTTCCGCCTCTGCTTTGGCTTTTGCCTCTTGTTTTGCTTTCTCGTCTGCCTGCACTTTGGCTTTTGCTTCTTCTTCCGCTTGTTTTGCAGCATCTGCTAAACGTTTTGCCTCAGCTTCAGCTTTGAGCTTAGCGGCTTCAGCAGCCTGTTTGGCTTTCGCCTCTTCTGCCTGTTTTTGCTTTTCCAAGGCTTCTAAGCGAGCTTTTTCTTCCTGTTGCTTTTTCTGTTCAGc                                                                                                                                                                                                                      >  1:780323/1‑250 (MQ=255)                           ctctGCTTTTGCCTTAGCTTCCGCCTCTGCTTTGGCTTTTGCCTCTTGTTTTGCTTTCTCGTCTGCCTGCACTTTGGCTTTTGCTTCTTCTTCCGCTTGTTTTGCAGCATCTGCTAAACGTTTTGCCTCAGCTTCAGCTTTGAGCTTAGCGGCTTCAGCAGCCTGTTTGGCTTTCGCCTCTTCTGCCTGTTTTTGCTTTTCCAAGGCTTCTAAGCGAGCTTTTTCTTCCTGTTGCTTTTTCTGTTCAGCCa                                                                                                                                                                                                                    <  1:399789/251‑1 (MQ=255)                               gCTTTTGCCTTAGCTTCCGCCTCTGCTTTGGCTTTTGCCTCTTGTTTTGCTTTCTCGTCTGCCTGCACTTTGGCTTTTGCTTCTTCTTCCGCTTGTTTTGCAGCATCTGCTAAACGTTTTGCCTCAGCTTCAGCTTTGAGCTTAGCGGCTTCAGCAGCCTGTTTGGCTTTCGCCTCTTCTGCCTGTTTTTGCTTTTCCAAGGCTTCTAAGCGAGCTTTTTCTTCCTGTTGCTTTTTCTGTTCAGCCAAGaa                                                                                                                                                                                                                >  1:759149/1‑251 (MQ=255)                                 ttttGCCTTAGCTTCCGCCTCTGCTTTGGCTTTTGCCTCTTGTTTTGCTTTCTCGTCTGCCTGCACTTTGGCTTTTGCTTCTTCTTCCGCTTGTTTTGCAGCATCTGCTAAACGTTTTGCCTCAGCTTCAGCTTTGAGCTTAGCGGCTTCAGCAGCCTGTTTGGCTTTCGCCTCTTCTGCCTGTTTTTGCTTTTCCAAGGCTTCTAAGCGAGCTTTTTCTTCCTGTTGCTTTTTCTGTTCAGCCAAGAAcc                                                                                                                                                                                                              <  1:51267/251‑1 (MQ=255)                                 ttttGCCTTAGCTTCCGCCTCTGCTTTGGCTTTTGCCTCTTGTTTTGCTTTCTCGTCTGCCTGCACTTTGGCTTTTGCTTCTTCTTCCGCTTGTTTTGCAGCATCTGCTAAACGTTTTGCCTCAGCTTCAGCTTTGAGCTTAGCGGCTTCAGCAGCCTGTTTGGCTTTCGCCTCTTCTGCCTGTTTTTGCTTTTCCAAGGCTTCTAAGCGAGCTTTTTCTTCCTGTTGCTTTTTCTGTTCAGCCAAGAAcc                                                                                                                                                                                                              <  1:555974/251‑1 (MQ=255)                                     gCCTTAGCTTCCGCCTCTGCTTTGGCTTTTGCCTCTTGTTTTGCTTTCTCGTCTGCCTGCACTTTGGCTTTTGCTTCTTCTTCCGCTTGTTTTGCAGCATCTGCTAAACGTTTTGCCTCAGCTTCAGCTTTGAGCTTAGCGGCTTCAGCAGCCTGTTTGGCTTTCGCCTCTTCTGCCTGTTTTTGCTTTTCCAAGGCTTCTAAGCGAGCTTTTTCTTCCTGTTGCTTTTTCTGTTCAGCCAAGAACCTTTg                                                                                                                                                                                                          >  1:121464/1‑251 (MQ=255)                                       cTTAGCTTCCGCCTCTGCTTTGGCTTTTGCCTCTTGTTTTGCTTTCTCGTCTGCCTGCACTTTGGCTTTTGCTTCTTCTTCCGCTTGTTTTGCAGCATCTGCTAAACGTTTTGCCTCAGCTTCAGCTTTGAGCTTAGCGGCTTCAGCAGCCTGTTTGGCTTTCGCCTCTTCTGCCTGTTTTTGCTTTTCCAAGGCTTCTAAGCGAGCTTTTTCTTCCTGTTGCTTTTTCTGTTCAGCCAAGAACCTTTGTc                                                                                                                                                                                                        <  1:793498/251‑1 (MQ=255)                                        ttAGCTTCCGCCTCTGCTTTGGCTTTTGCCTCTTGTTTTGCTTTCTCGTCTGCCTGCACTTTGGCTTTTGCTTCTTCTTCCGCTTGTTTTGCAGCATCTGCTAAACGTTTTGCCTCAGCTTCAGCTTTGAGCTTAGCGGCTTCAGCAGCCTGTTTGGCTTTCGCCTCTTCTGCCTGTTTTTGCTTTTCCAAGGCTTCTAAGCGAGCTTTTTCTTCCTGTTGCTTTTTCTGTTCAGCCAAGAACCTTTGTCt                                                                                                                                                                                                       <  1:647367/251‑1 (MQ=255)                                           gCTTCCGCCTCTGCTTTGGCTTTTGCCTCTTGTTTTGCTTTCTCGTCTGCCTGCACTTTGGCTTTTGCTTCTTCTTCCGCTTGTTTTGCAGCATCTGCTAAACGTTTTGCCTCAGCTTCAGCTTTGAGCTTAGCGGCTTCAGCAGCCTGTTTGGCTTTCGCCTCTTCTGCCTGTTTTTGCTTTTCCAAGGCTTCTAAGCGAGCTTTTTCTTCCTGTTGCTTTTTCTGTTCAGCCAAGAACCTTTGTCttt                                                                                                                                                                                                     >  1:356849/1‑250 (MQ=255)                                               ccGCCTCTGCTTTGGCTTTTGCCTCTTGTTTTGCTTTCTCGTCTGCCTGCACTTTGGCTTTTGCTTCTTCTTCCGCTTGTTTTGCAGCATCTGCTAAACGTTTTGCCTCAGCTTCAGCTTTGAGCTTAGCGGCTTCAGCAGCCTGTTTGGCTTTCGCCTCTTCTGCCTGTTTTTGCTTTTCCAAGGCTTCTAAGCGAGCTTTTTCTTCCTGTTGCTTTTTCTGTTCAGCCAAGAACCTTTGTCTTTCCAGc                                                                                                                                                                                                <  1:512124/251‑1 (MQ=255)                                                cGCCTCTGCTTTGGCTTTTGCCTCTTGTTTTGCTTTCTCGTCTGCCTGCACTTTGGCTTTTGCTTCTTCTTCCGCTTGTTTTGCAGCATCTGCTAAACGTTTTGCCTCAGCTTCAGCTTTGAGCTTAGCGGCTTCAGCAGCCTGTTTGGCTTTCGCCTCTTCTGCCTGTTTTTGCTTTTCCAAGGCTTCTAAGCGAGCTTTTTCTTCCTGTTGCTTTTTc                                                                                                                                                                                                                              <  1:817395/220‑1 (MQ=255)                                                  ccTCTGCTTTGGCTTTTGCCTCTTGTTTTGCTTTCTCGTCTGCCTGCACTTTGGCTTTTGCTTCTTCTTCCGCTTGTTTTGCAGCATCTGCTAAACGTTTTGCCTCAGCTTCAGCTTTGAGCTTAGCGGCTTCAGCAGCCTGTTTGGCTTTCGCCTCTTCTGCCTGTTTTTGCTTTTCCAAGGCTTCTAAGCGAGCTTTTTCTTCCTGTTGCTTTTTCTGTTCAGCCAAGAACCTTTGTCTTTCCAGct                                                                                                                                                                                               >  1:672195/1‑249 (MQ=255)                                                       gCTTTGGCTTTTGCCTCTTGTTTTGCTTTCTCGTCTGCCTGCACTTTGGCTTTTGCTTCTTCTTCCGCTTGTTTTGCAGCATCTGCTAAACGTTTTGCCTCAGCTTCAGCTTTGAGCTTAGCGGCTTCAGCAGCCTGTTTGGCTTTCGCCTCTTCTGCCTGTTTTTGCTTTTCCAAGGCTTCTAAGCGAGCTTTTTCTTCCTGTTGCTTTTTCTGTTCAGCCAAGAACCTTTGTCTTTCCAGCTCTTTTTg                                                                                                                                                                                        >  1:178956/1‑251 (MQ=255)                                                       gCTTTGGCTTTTGCCTCTTGTTTTGCTTTCTCGTCTGCCTGCACTTTGGCTTTTGCTTCTTCTTCCGCTTGTTTTGCAGCATCTGCTAAACGTTTTGCCTCAGCTTCAGCTTTGAGCTTAGCGGCTTCAGCAGCCTGTTTGGCTTTCGCCTCTTCTGCCTGTTTTTGCTTTTCCAAGGCTTCTAAGCGAGCTTTTTCTTCCTGTTGCTTTTTCTGTTCAGCCAAGAACCTTTGTCTTTCCAGCTCTTTTTg                                                                                                                                                                                        >  1:298726/1‑251 (MQ=255)                                                        cTTTGGCTTTTGCCTCTTGTTTTGCTTTCTCGTCTGCCTGCACTTTGGCTTTTGCTTCTTCTTCCGCTTGTTTTGCAGCATCTGCTAAACGTTTTGCCTCAGCTTCAGCTTTGAGCTTAGCGGCTTCAGCAGCCTGTTTGGCTTTCGCCTCTTCTGCCTGTTTTTGCTTTTCCAAGGCTTCTAAGCGAGCTTTTTCTTCCTGTTGCTTTTTCTGTTCAGCCAAGAACCTTTGTCTTTCCAGCTCTTTTTGc                                                                                                                                                                                       <  1:303355/251‑1 (MQ=255)                                                         tttGGCTTTTGCCTCTTGTTTTGCTTTCTCGTCTGCCTGCACTTTGGCTTTTGCTTCTTCTTCCGCTTGTTTTGCAGCATCTGCTAAACGTTTTGCCTCAGCTTCAGCTTTGAGCTTAGCGGCTTCAGCAGCCTGTTTGGCTTTCGCCTCTTCTGCCTGTTTTTGCTTTTCCAAGGCTTCTAAGCGAGCTTTTTCTTCCTGTTGCTTTTCCTGTTCAGCCAAGAACCTTTGTCTTTCCAGCTCTTTTTGCt                                                                                                                                                                                      <  1:622393/251‑1 (MQ=255)                                                          ttGGCTTTTGCCTCTTGTTTTGCTTTCTCGTCTGCCTGCACTTTGGCTTTTGCTTCTTCTTCCGCTTGTTTTGCAGCATCTGCTAAACGTTTTGCCTCAGCTTCAGCTTTGAGCTTAGCGGCTTCAGCAGCCTGTTTGGCTTTCGCCTCTTCTGCCTGTTTTTGCTTTTCCAAGGCTTCTAAGCGAGCTTTTTCTTCCTGTTGCTTTTTCTGTTCAGCCAAGAACCTTTGTCTTTCCAGCTCTTTTTGCTg                                                                                                                                                                                     <  1:823075/251‑1 (MQ=255)                                                                  tGCCTCTTGTTTTGCTTTCTCGTCTGCCTGCACTTTGGCTTTTGCTTCTTCTTCCGCTTGTTTTGCAGCATCTGCTAAACGTTTTGCCTCAGCTTCAGCTTTGAGCTTAGCGGCTTCAGCAGCCTGTTTGGCTTTCGCCTCTTCTGCCTGTTTTTGCTTTTCCAAGGCTTCTAAGCGAGCTTTTTCTTCCTGTTGCTTTTTCTGTTCAGCCAAGAACCTTTGTCTTTCCAGCTCTTTTTGCTGTTCCAGtt                                                                                                                                                                             <  1:342131/251‑1 (MQ=255)                                                                    ccTCTTGTTTTGCTTTCTCGTCTGCCTGCACTTTGGCTTTTGCTTCTTCTTCCGCTTGTTTTGCAGCATCTGCTAAACGTTTTGCCTCAGCTTCAGCTTTGAGCTTAGCGGCTTCAGCAGCCTGTTTGGCTTTCGCCTCTTCTGCCTGTTTTTGCTTTTCCAAGGCTTCTAAGCGAGCTTTTTCTTCCTGTTGCTTTTTCTGTTCAGCCAAGAACCTTTGTCTTTCCAGCTCTTTTTGCTGTTCCAGttc                                                                                                                                                                            <  1:758665/250‑1 (MQ=255)                                                                       ctTGTTTTGCTTTCTCGTCTGCCTGCACTTTGGCTTTTGCTTCTTCTTCCGCTTGTTTTGCAGCATCTGCTAAACGTTTTGCCTCAGCTTCAGCTTTGAGCTTAGCGGCTTCAGCAGCCTGTTTGGCTTTCGCCTCTTCTGCCTGTTTTTGCTTTTCCAAGGCTTCTAAGCGAGCTTTTTCTTCCTGTTGCTTTTTCTGTTCAGCCAAGAACCTTTGTCTTTCCAGCTCTTTTTGCTGTTCCAGttcttt                                                                                                                                                                         >  1:713054/1‑250 (MQ=255)                                                                               gCTTTCTCGTCTGCCTGCACTTTGGCTTTTGCTTCTTCTTCCGCTTGTTTTGCAGCATCTGCTAAACGTTTTGCCTCAGCTTCAGCTTTGAGCTTAGCGGCTTCAGCAGCCTGTTTGGCTTTCGCCTCTTCTGCCTGTTTTTGCTTTTCCAAGGCTTCTAAGCGGGCTTTTTCTTCCTGTTGCTTTTTCTGTTCAGCCAAGAAcc                                                                                                                                                                                                              >  1:799390/1‑205 (MQ=255)                                                                                 tttCTCGTCTGCCTGCACTTTGGCTTTTGCTTCTTCTTCCGCTTGTTTTGCAGCATCTGCTAAACGTTTTGCCTCAGCTTCAGCTTTGAGCTTAGCGGCTTCAGCAGCCTGTTTGGCTTTCGCCTCTTCTGCCTGTTTTTGCTTTTCCAAGGCTTCTAAGCGAGCTTTTTCTTCCTGTTGCTTTTTCTGTTCAGCCAAGAACCTTTGTCTTTCCAGCTCTTTTTGCTGTTCCAGTTCTTTCTGACGGGCaa                                                                                                                                                              >  1:59645/1‑251 (MQ=255)                                                                                    ctcGTCTGCCTGCACTTTGGCTTTTGCTTCTTCTTCCGCTTGTTTTGCAGCATCTGCTAAACGTTTTGCCTCAGCTTCAGCTTTGAGCTTAGCGGCTTCAGCAGCCTGTTTGGCTTTCGCCTCTTCTGCCTGTTTTTGCTTTTCCAAGGCTTCTAAGCGAGCTTTTTCTTCCTGTTGCTTTTTCTGTTCAGCCAAGAACCTTTGTCTTTCCAGCTCTTTTTGCTGTTCCAGTTCTTTCTGACGGGCAAttt                                                                                                                                                           <  1:368024/251‑1 (MQ=255)                                                                                      cGTCTGCCTGCACTTTGGCTTTTGCTTCTTCTTCCGCTTGTTTTGCAGCATCTGCTAAACGTTTTGCCTCAGCTTCAGCTTTGAGCTTAGCGGCTTCAGCAGCCTGTTTGGCTTTCGCCTCTTCTGCCTGTTTTTGCTTTTCCAAGGCTTCTAAGCGAGCTTTTTCTTCCTGTTGCTTTTTCTGTTCAGCCAAGAACCTTTGTCTTTCCAGCTCTTTTTGCTGTTCCAGTTCTTTCTGACGGGCAATTTcc                                                                                                                                                         <  1:875155/251‑1 (MQ=255)                                                                                         ctgcctgcACTTTGGCTTTTGCTTCTTCTTCCGCTTGTTTTGCAGCATCTGCTAAACGTTTTGCCTCAGCTTCAGCTTTGAGCTTAGCGGCTTCAGCAGCCTGTTTGGCTTTCGCCTCTTCTGCCTGTTTTTGCTTTTCCAAGGCTTCTAAGCGAGCTTTTTCTTCCTGTTGCTTTTTCTGTTCAGCCAAGAACCTTTGTCTTTCCAGCTCTTTTTGCTGTTCCAGTTCTTTCTGACGGGCAATTTc                                                                                                                                                          >  1:741923/1‑247 (MQ=255)                                                                                          tgcctgcACTTTGGCTTTTGCTTCTTCTTCCGCTTGTTTTGCAGCATCTGCTAAACGTTTTGCCTCAGCTTCAGCTTTGAGCTTAGCGGCTTCAGCAGCCTGTTTGGCTTTCGCCTCTTCTGCCTGTTTTTGCTTTTCCAAGGCTTCTAAGCGAGCTTTTTCTTCCTGTTGCTTTTTCTGTTCAGCCAAGAACCTTTGTCTTTCCAGCTCTTTTTGCTGTTCCAGTTCTTTCTGACGGGCAATTTCCTGtt                                                                                                                                                     >  1:425276/1‑251 (MQ=255)                                                                                                      ggCTTTTGCTTCTTCTTCCGCTTGTTTTGCAGCATCTGCTAAACGTTTTGCCTCAGCTTCAGCTTTGAGCTTAGCGGCTTCAGCAGCCTGTTTGGCTTTCGCCTCTTCTGCCTGTTTTTGCTTTTCCAAGGCTTCTAAGCGAGCTTTTTCTTCCTGTTGCTTTTTCTGTTCAGCCAAGAACCTTTGTCTTTCCAGCTCTTTTTGCt                                                                                                                                                                                      >  1:58392/1‑206 (MQ=255)                                                                                                            tGCTTCTTCTTCCGCTTGTTTTGCAGCATCTGCTAAACGTTTTGCCTCAGCTTCAGCTTTGAGCTTAGCGGCTTCAGCAGCCTGTTTGGCTTTCGCCTCTTCTGCCTGTTTTTGCTTTTCCAAGGCTTCTAAGCGAGCTTTTTCTTCCTGTTGCTTTTTCTGTTCAGCCAAGAACCTTTGTCTTTCCAGCTCTTTTTGCTGTTCCAGTTCTTTCTGACGGGCAATTTCCTGTTGACGTTGTTGCTCTTTTa                                                                                                                                   <  1:247837/251‑1 (MQ=255)                                                                                                              cttcttcttCCGCTTGTTTTGCAGCATCTGCTAAACGTTTTGCCTCAGCTTCAGCTTTGAGCTTAGCGGCTTCAGCAGCCTGTTTGGCTTTCGCCTCTTCTGCCTGTTTTTGCTTTTCCAAGGCTTCTAAGCGAGCTTTTTCTTCCTGTTGCTTTTTCTGTTCAGCCAAGAACCTTTGTCTTTCCAGCTCTTTTTGCTGTTCCAGTTCTTTCTGACGGGCAATTTCCTGTTGACGTTGTTGCTCTTTTAac                                                                                                                                 <  1:560429/251‑1 (MQ=255)                                                                                                               ttcttcttcCGCTTGTTTTGCAGCGTCGGTTAAACGTTTTGCTTCGGCTTCGGCTTTGAGCTTAGGGGCTTCAGCAGCTTGTTTGGCTTTCGCCTCTTCTACC‑GGTTTTGCTTTTCCAAGGCTTCTAAGCGAGATTTTTCTTCCTGTTGCTTTTTCTGTTCAGCCAAGAACCTTTGTCTTTCCAGCTCTTTTTGCTGTTCCAGTTCTTTCTGACGGGCAATTTCCTGTTGACGTTGTTGCTCTTTTAacac                                                                                                                               <  1:283585/251‑1 (MQ=255)                                                                                                               ttcttcttcCGCTTGTTTTGCAGCATCTGCTAAACGTTTTGCCTCAGCTTCAGCTTTGAGCTTAGCGGCTTCAGCAGCCTGTTTGGCTTTCGCCTCTTCTGCCTGTTTTTGCTTTTCCAAGGCTTCTAAGCGAGCTTTTTCTTCCTGTTGCTTTTTCTGTTCAGCCAAGAACCTTTGTCTTTCCAGCTCTTTTTGCTGTTCCAGttc                                                                                                                                                                            <  1:647171/207‑1 (MQ=255)                                                                                                                tcttcttcCGCTTGTTTTGCAGCATCTGCTAAACGTTTTGCCTCAGCTTCAGCTTTGAGCTTAGCGGCTTCAGCAGCCTGTTTGGCTTTCGCCTCTTCTGCCTGTTTTTGCTTTTCCAAGGCTTCTAAGCGAGCTTTTTCTTCCTGTTGCTTTTTCTGTTCAGCCAAGAACCTTTGTCTTTCCAGCTCTTTTTGCTGTTCCAGTTCTTTCTGACGGGCAATTTCCTGTTGACGTTGTTGCTCTTTTAacac                                                                                                                               <  1:61223/251‑1 (MQ=255)                                                                                                                tcttcttcCGCTTGTTTTGCAGCATCTGCTAAACGTTTTGCCTCAGCTTCAGCTTTGAGCTTAGCGGCTTCAGCAGCCTGTTTGGCTTTCGCCTCTTCTGCCTGTTTTTGCTTTTCCAAGGCTTCTAAGCGAGCTTTTTCTTCCTGTTGCTTTTTCTGTTCAGCCAAGAACCTTTGTCTTTCCAGCTCTTTTTGCTGTTCCAGTTCTTTCTGACGGGCAATTTCCTGTTGACGTTGTTGCTCTTTTAacac                                                                                                                               <  1:672584/251‑1 (MQ=255)                                                                                                                tcttcttcCGCTTGTTTTGCAGCATCTGCTAAACGTTTTGCCTCAGCTTCAGCTTTGAGCTTAGCGGCTTCAGCAGCCTGTTTGGCTTTCGCCTCTTCTGCCTGTTTTTGCTTTTCCAAGGCTTCTAAGCGAGCTTTTTCTTCCTGTTGCTTTTTCTGTTCAGCCAAGAACCTTTGTCTTTCCAGCTCTTTTTGCTGTTCCAGTTCTTTCTGACGGGCAATTTCCTGTTGACGTTGTTGCTCTTTTAacac                                                                                                                               <  1:765384/251‑1 (MQ=255)                                                                                                                tcttcttcCGCTTGTTTTGCAGCATCTGCTAAACGTTTTGCCTCAGCTTCAGCTTTGAGCTTAGCGGCTTCAGCAGCCTGTTTGGCTTTCGCCTCTTCTGCCTGTTTTTGCTTTTCCAAGGCTTCTAAGCGAGCTTTTTCTTCCTGTTGCTTTTTCTGTTCAGCCAAGAACCTTTGTCTTTCCAGCTCTTTTTGCTGTTCCAGTTCTTTCTGACGGGCAATTTCCTGTTGACGTTGTTGCTCTTTTAacac                                                                                                                               <  1:880643/251‑1 (MQ=255)                                                                                                                             gTTTTGCAGCATCTGCTAAACGTTTTGCCTCAGCTTCAGCTTTGAGCTTAGCGGCTTCAGCAGCCTGTTTGGCTTTCGCCTCTTCTGCCTGTTTTTGCTTTTCCAAGGCTTCTAAGCGAGCTTTTTCTTCCTGTTGCTTTTTCTGTTCAGCCAAGAACCTTTGTCTTTCCAGCTCtttt                                                                                                                                                                                          >  1:691476/1‑179 (MQ=255)                                                                                                                             gTTTTGCAGCATCTGCTAAACGTTTTGCCTCAGCTTCAGCTTTGAGCTTAGCGGCTTCAGCAGCCTGTTTGGCTTTCGCCTCTTCTGCCTGTTTTTGCTTTTCCAAGGCTTCTAAGCGAGCTTTTTCTTCCTGTTGCTTTTTCTGTTCAGCCAAGAACCTTTGTCTTTCCAGCTCTTTTTGCTGTTCCAGTTCTTTCTGACGGGCAATTTCCTGTTGACGTTGTTGCTCTTTTAacac                                                                                                                               <  1:861750/238‑1 (MQ=255)                                                                                                                                ttGCAGCATCTGCTAAACGTTTTGCCTCAGCTTCAGCTTTGAGCTTAGCGGCTTCAGCAGCCTGTTTGGCTTTCGCCTCTTCTGCCTGTTTTTGCTTTTCCAAGGCTTCTAAGCGAGCTTTTTCTTCCTGTTGCTTTTTCTGTTCAGCCAAGAACCTTTGTCTTTCCAGCTCTTTTTGCTGTTCCAGTTCTTTCTGACGGGCAATTTCCTGTTGACGTTGTTGCTCTTTTAACACTTCCCGTTGttttt                                                                                                                 >  1:795655/1‑249 (MQ=255)                                                                                                                                       aTCTGCTAAGCGTTTTGCCTCAGCTTCAGCTTTGAGCTTAGCGGCTTCAGCAGCCTGTTTGGCTTTCGCCTCTTCTGCCTGTTTTTGCTTTTCCAAGGCTTCTAAGCGAGCTTTTTCTTCCTGTTGCTTTTTCTGTTCAGCCAAGAACCTTTGTCTTTCCAGCTCTTTTTGCTGTTCCAGTTCTTTCTGACGGGCAATTTCCTGTTGACGTTGTTGCTCTTTTAACACTTCCCGTTGTTTTTCTTCTTccc                                                                                                        <  1:226199/251‑1 (MQ=255)                                                                                                                                           gCTAAACGTTTTGCCTCAGCTTCAGCTTTGAGCTTAGCGGCTTCAGCAGCCTGTTTGGCTTTCGCCTCTTCTGCCTGTTTTTGCTTTTCCAAGGCTTCTAAGCGAGCTTTTTCTTCCTGTTGCTTTTTCTGTTCAGCCAAGAACCTTTGTCTTTCCAGctc                                                                                                                                                                                              <  1:180879/161‑1 (MQ=255)                                                                                                                                           gCTAAACGTTTTGCCTCAGCTTCAGCTTTGAGCTTAGCGGCTTCAGCAGCCTGTTTGGCTTTCGCCTCTTCTGCCTGTTTTTGCTTTTCCAAGGCTTCTAAGCGAGCTTTTTCTTCCTGTTGCTTTTTCTGTTCAGCCAAGAACCTTTGTCTTTCCAGCTCTTTTTGCTGTTCCAGTTCTTTCTGACGGGCAATTTCCTGTTGACGTTGTTGCTCTTTTAACACTTCCCGTTGTTTTTCTTCTTCCCGttt                                                                                                    >  1:793076/1‑251 (MQ=255)                                                                                                                                                aCGTTTTGCCTCAGCTTCAGCTTTGAGCTTAGCGGCTTCAGCAGCCTGTTTGGCTTTCGCCTCTTCTGCCTGTTTTTGCTTTTCCAAGGCTTCTAAGCGAGCTTTTTCTTCCTGTTGCTTTTTCTGTTCAGCCAAGAACCTTTGTCTTTCCAGCTCTTTTTGCTGTTCCAGTTCTTTCTGACGGGCAATTTCCTGTTGACGTTGTTGCTCTTTTAACACTTCCCGTTGTTTTTCTTCTTCCCGTTTCTg                                                                                                 >  1:138422/1‑249 (MQ=255)                                                                                                                                                            agcttcagcttTGAGCTTAGCGGCTTCAGCAGCCTGTTTGGCTTTCGCCTCTTCTGCCTGTTTTTGCTTTTCCAAGGCTTCTAAGCGAGCTTTTTCTTCCTGTTGCTTTTTCTGTTCAGCCAAGAACCTTTGTCTTTCCAGCTCTTTTTGCTGTTCCAGTTCTTTCTGACGGGCAATTTCCTGTTGACGTTGTTGCTCTTTTAACACTTCCCGTTGTTTTTCTTCTTCCCGTTTCTGCTCTTCACGttttt                                                                                   >  1:796201/1‑251 (MQ=255)                                                                                                                                                             gcttcagcttTGAGCTTAGCGGCTTCAGCAGCCTGTTTGGCTTTCGCCTCTTCTGCCTGTTTTTGCTTTTCCAAGGCTTCTAAGCGAGCTTTTTCTTCCTGTTGCTTTTTCTGTTCAGCCAAGAACCTTTGTCTTTCCAGCTCTTTTTGCTGTTCCAGtt                                                                                                                                                                             <  1:230254/160‑1 (MQ=255)                                                                                                                                                                  agcttTGAGCTTAGCGGCTTCAGCAGCCTGTTTGGCTTTCGCCTCTTCTGCCTGTTTTTGCTTTTCCAAGGCTTCTAAGCGAGCTTTTTCTTCCTGTTGCTTTTTCTGTTCAGCCAAGAACCTTTGTCTTTCCAGCTCTTTTTGCTGTTCCAGTTCTTTCTGACGGGCAATTTCCTGTTGACGTTGTTGCTCTTTTAACACTTCCCGTTGTTTTTCTTCTTCCCGttt                                                                                                    <  1:121767/228‑1 (MQ=255)                                                                                                                                                                   gcttTGAGCTTAGCGGCTTCAGCAGCCTGTTTGGCTTTCGCCTCTTCTGCCTGTTTTTGCTTTTCCAAGGCTTCTAAGCGAGCTTTTTCTTCCTGTTGCTTTTTCTGTTCAGCCAAGAACCTTTGTCTTTCCAGCTCTTTTTGCTGTTCCAGTTCTTTCTGa                                                                                                                                                                     <  1:780565/162‑1 (MQ=255)                                                                                                                                                                     tttGAGCTTAGCGGCTTCAGCAGCCTGTTTGGCTTTCGCCTCTTCTGCCTGTTTTTGCTTTTCCAAGGCTTCTAAGCGAGCTTTTTCTTCCTGTTGCTTTTTCTGTTCAGCCAAGAACCTTTGTCTTTCCAGCTCTTTTTGCTGTTCCAGTTCTTTCTGACGGGCAATTTCCTGTTGACGTTGTTGCTCTTTTAACACTTCCCGTTGTTTTTCTTCTTCCCGTTTCTGCTCTTCACGTTTTTGGTCTTCaa                                                                          >  1:167165/1‑251 (MQ=255)                                                                                                                                                                      ttGAGCTTAGCGGCTTCAGCAGCCTGTTTGGCTTTCGCCTCTTCTGCCTGTTTTTGCTTTTCCAAGGCTTCTAAGCGAGCTTTTTCTTCCTGTTGCTTTTTCTGTTCAGCCAAGAACCTTTGTCTTTCCAGCTCTTTTTGCTGTTCCAGTTCTTTCTGACGGGCAATTTCCTGTTGACGTTGTTGCTCTTTTAACACTTCCCGTTGTTTTTCTTCTTCCCGTTTCTGCTCTTCACGTTTTTGGTCTTCAAg                                                                         <  1:760541/251‑1 (MQ=255)                                                                                                                                                                      ttGAGCTTAGCGGCTTCAGCAGCCTGTTTGGCTTTCGCCTCTTCTGCCTGTTTTTGCTTTTCCAAGGCTTCTAAGCGAGCTTTTTCTTCCTGTTGCTTTTTCTGTTCAGCCAAGAACCTTTGTCTTTCCAGCTCTTTTTGCTGTTCCAGTTCTTTCTGACGGGCAATTTCCTGTTGACGTTGTTGCTCTTTTAACACTTCCCGTTGTTTTTCTTCTTCCCGTTTCTGCTCTTCACGTTTTTGGTCTTCAAg                                                                         <  1:270783/251‑1 (MQ=255)                                                                                                                                                                                 ggCTTCAGCAGCCTGTTTGGCTTTCGCCTCTTCTGCCTGTTTTTGCTTTTCCAAGGCTTCTAAGCGAGCTTTTTCTTCCTGTTGCTTTTTCTGTTCAGCCAAGAACCTTTGTCTTTCCAGCTCTTTTTGCTGTTCCAGTTCTTTCTGACGGGCAATTTCCTGTTGACGTTGTTGCTCTTTTAACACTTCCCGTTGTTTTTCTTCTTCCCGTTTCTGCTCTTCACGTTTTTGGTCTTCAAGGGCTTGttt                                                                >  1:240959/1‑249 (MQ=255)                                                                                                                                                                                  gCTTCAGCAGCCTGTTTGGCTTTCGCCTCTTCTGCCTGTTTTTGCTTTTCCAAGGCTTCTAAGCGAGCTTTTTCTTCCTGTTGCTTTTTCTGTTCAGCCAAGAACCTTTGTCTTTCCAGCTCTTTTTGCTGTTCCAGTTCTTTCTGACGGGCAATTTCCTGTTGACGTTGTTGCTCTTTTAACACTTCCCGTTGTTTTTCTTCTTCCCGTTTCTGCTCTTCACGTTTTTGGTCTTCAAGGGCTTGTTTCtt                                                             >  1:171573/1‑251 (MQ=255)                                                                                                                                                                                   cTTCAGCAGCCTGTTTGGCTTTCGCCTCTTCTGCCTGTTTTTGCTTTTCCAAGGCTTCTAAGCGAGCTTTTTCTTCCTGTTGCTTTTTCTGTTCAGCCAAGAACCTTTGTCTTTCCAGCTCTTTTTGCTGTTCCAGTTCTTTCTGACGGGCAATTTCCTGTTGACGTTGTTGCTCTTTTAACACTTCCCGTTGTTTTTCTTCTTCCCGTTTCTGCTCTTCACGTTTTTGGTCTTCAAGGGCTTGTTTCttt                                                            <  1:502529/251‑1 (MQ=255)                                                                                                                                                                                     tCAGCAGCCTGTTTGGCTTTCGCCTCTTCTGCCTGTTTTTGCTTTTCCAAGGCTTCTAAGCGAGCTTTTTCTTCCTGTTGCTTTTTCTGTTCAGCCAAGAACCTTTGTCTTTCCAGCTCTTTTTGCTGTTCCAGTTCTTTCTGACGGGCAATTTCCTGTTGACGTTGTTGCTCTTTTAacac                                                                                                                               <  1:91589/182‑1 (MQ=255)                                                                                                                                                                                     tCAGCAGCCTGTTTGGCTTTCGCCTCTTCTGCCTGTTTTTGCTTTTCCAAGGCTTCTAAGCGAGCTTTTTCTTCCTGTTGCTTTTTCTGTTCAGCCAAGAACCTTTGTCTTTCCAGCTCTTTTTGCTGTTCCAGTTCTTTCTGACGGGCAATTTCCTGTTGACGTTGTTGCTCTTTTAACACTTCCCGTTGTTTTTCTTCTTCCCGTTTCTGCTCTTCACGTTTTTGGTCTTCAAGGGCTTGtt                                                                 >  1:80255/1‑244 (MQ=255)                                                                                                                                                                                     tCAGCAGCCTGTTTGGCTTTCGCCTCTTCTGCCTGTTTTTGCTTTTCCAAGGCTTCTAAGCGAGCTTTTTCTTCCTGTTGCTTTTTCTGTTCAGCCAAGAACCTTTGTCTTTCCAGCTCTTTTTGCTGTTCCAGTTCTTTCTGACGGGCAATTTCCTGTTGACGTTGTTGCTCTTTTAACACTTCCCGTTGTTTTTCTTCTTCCCGTTTCTGCTCTTCACGTTTTTGGTCTTCAAGGGCTTGTTTCTTTtg                                                          <  1:194894/251‑1 (MQ=255)                                                                                                                                                                                      cagcagCCTGTTTGGCTTTCGCCTCTTCTGCCTGTTTTTGCTTTTCCAAGGCTTCTAAGCGAGCTTTTTCTTCCTGTTGCTTTTTCTGTTCAGCCAAGAACCTTTGTCTTTCCAGCTCTTTTTGCTGTTCCAGtt                                                                                                                                                                             <  1:166881/135‑1 (MQ=255)                                                                                                                                                                                         cagcCTGTTTGGCTTTCGCCTCTTCTGCCTGTTTTTGCTTTTCCAAGGCTTCTAAGCGAGCTTTTTCTTCCTGTTGCTTTTTCTGTTCAGCCAAGAACCTTTGTCTTTCCAGCTCTTTTTGCTGTTCCAGTTCTTTCTGACGGGCAATTTCCTGTTGACGTTGTTGCTCTTTTAACACTTCCCGTTGTTTTTCTTCTTCCCGTTTCTGCTCTTCACGTTTTTGGTCTTCAAGGGCTTGTTTCTTTtgtc                                                        <  1:150156/249‑1 (MQ=255)                                                                                                                                                                                         cagcCTGTTTGGCTTTCGCCTCTTCTGCCTGTTTTTGCTTTTCCAAGGCTTCTAAGCGAGCTTTTTCTTCCTGTTGCTTTTTCTGTTCAGCCAAGAACCTTTGTCTTTCCAGCTCTTTTTGCTGTTCCAGTTCTTTCTGACGGGCAATTTCCTGTTGACGTTGTTGCTCTTTTAACACTTCCCGTTGTTTTTCTTCTTCCCGTTTCTGCTCTTCACGTTTTTGGTCTTCAAGGGCTTGTTTCTTTtgtc                                                        <  1:750531/249‑1 (MQ=255)                                                                                                                                                                                             cTGTTTGGCTTTCGCCTCTTCTGCCTGTTTTTGCTTTTCCAAGGCTTCTAAGCGAGCTTTTTCTTCCTGTTGCTTTTTCTGTTCAGCCAAGAACCTTTGTCTTTCCAGCTCTTTTTGCTGTTCCAGTTCTTTCTGACGGGCAATTTCCTGTTGACGTTGTTGCTCTTTTAACACTTCCCGTTGTTTTTCTTCTTCCCGTTTCTGCTCTTCACGTTTTTGGTCTTCAAGGGCTTGTTTCTTTTGTCTGTCCg                                                  <  1:878281/251‑1 (MQ=255)                                                                                                                                                                                               gTTTGGCTTTCGCCTCTTCTGCCTGTTTTTGCTTTTCCAAGGCTTCTAAGCGAGCTTTTTCTTCCTGTTGCTTTTTCTGTTCAGCCAAGAACCTTTGTCTTTCCAGCTCTTTTTGCTGTTCCAGTTCTTTCTGACGGGCAATTTCCTGTTGACGTTGTTGCTCTTTTAACACTTCCCGTTGTTTTTCTTCTTcc                                                                                                         <  1:494522/194‑1 (MQ=255)                                                                                                                                                                                                    gCTTTCGCCTCTTCTGCCTGTTTTTGCTTTTCCAAGGCTTCTAAGCGAGCTTTTTCTTCCTGTTGCTTTTTCTGTTCAGCCAAGAACCTTTGTCTTTCCAGCTCTTTTTGCTGTTCCAGTTCTTTCTGACGGGCAATTTCCTGTTGACGTTGTTGCTCTTTTAACACTTCCCGTTGTTTTTCTTCTTCCCGTTTCTGCTCTTCACGTTTTTGGTCTTCAAGGGCTTGTTTCTTTtgtct                                                       >  1:416962/1‑239 (MQ=255)                                                                                                                                                                                                      tttCGCCTCTTCTGCCTGTTTTTGCTTTTCCAAGGCTTCTAAGCGAGCTTTTTCTTCCTGTTGCTTTTTCTGTTCAGCCAAGAACCTTTGTCTTTCCAGCTCTTTTTGCTGTTCCAGTTCTTTCTGACGGGCAATTTCCTGTTGACGTTGTTGCTCTTTTAACACTTCCCGTTGTTTTTCTTCTTCCCGTTTCTGCTCTTCACGTTTTTGGTCTTCAAggg                                                                       >  1:886507/1‑221 (MQ=255)                                                                                                                                                                                                         cGCCTCTTCTGCCTGTTTTTGCTTTTCCAAGGCTTCTAAGCGAGCTTTTTCTTCCTGTTGCTTTTTCTGTTCAGCCAAGAACCTTTGTCTTTCCAGCTCTTTTTGCTGTTCCAGTTCTTTCTGACGGGCAATTTCCTGTTGACGTTGTTGCTCTTTTAACACTTCCCGTTGTTTttcttc                                                                                                             <  1:41125/180‑1 (MQ=255)                                                                                                                                                                                                         cGCCTCTTCTGCCTGTTTTTGCTTTTCCAAGGCTTCTAAGCGAGCTTTTTCTTCCTGTTGCTTTTTCTGTTCAGCCAAGAACCTTTGTCTTTCCAGCTCTTTTTGCTGTTCCAGTTCTTTCTGACGGGCAATTTCCTGTTGACGTTGTTGCTCTTTTAACACTTCCCGTTGTTTTTCTTCTTCCCGTTTCTGCTCTTCACGTTTTTGGTCTTCAAGGGCTTGTTTCTTTTGTCTGTCCGCCTGACCttttt                                      >  1:711396/1‑251 (MQ=255)                                                                                                                                                                                                             tcttctGCCTGTTTTTGCTTTTCCAAGGCTTCTAAGCGAGCTTTTTCTTCCTGTTGCTTTTTCTGTTCAGCCAAGAACCTTTGTCTTTCCAGCTCTTTTTGCTGTTCCAGTTCTTTCTGACGGGCAATTTCCTGTTGACGTTGTTGCTCTTTTAACACTTCCCGTTGTTTTTCTTCTTCCCGTTTCTGCTCTTCACGTTTTTGGTCTTCAAGGGCTTGTTTCTTTTGTCTGTCCGCCTGACCTTTTTTCtg                                  <  1:329667/251‑1 (MQ=255)                                                                                                                                                                                                                           ttGCTTTTCCAAGGCTTCTAAGCGAGCTTTTTCTTCCTGTTGCTTTTTCTGTTCAGCCAAGAACCTTTGTCTTTCCAGCTCTTTTTGCTGTTCCAGTTCTTTCTGACGGGCAATTTCCTGTTGACGTTGTTGCTCTTTTAACACTTCCCGTTGTTTTTCTTCTTCCCGTTTCTGCTCTTCACGTTTTTGGTCTTCAAGGGCTTGTTTCTTTTGTCTGTCCGCCTGACCTTTTTTCTGTTGTTGAATTCGcc                    <  1:222842/251‑1 (MQ=255)                                                                                                                                                                                                                              cTTTTCCAAGGCTTCTAAGCGAGCTTTTTCTTCCTGTTGCTTTTTCTGTTCAGCCAAGAACCTTTGTCTTTCCAGCTCTTTTTGCTGTTCCAGTTCTTTCTGACGGGCAATTTCCTGTTGACGTTGTTGCTCTTTTAACACTTCCCGTTGTTTTTCTTCTTCCCGTTTCTGCTCTTCACGTTTTTGGTCTTCAAGGGCTTGTTTCTTTTGTCTGTCCGCCTGACCTTTTTTCTGTTGTTGAATTCGcccc                  <  1:154936/250‑1 (MQ=255)                                                                                                                                                                                                                                tttCCAAGGCTTCTAAGCGAGCTTTTTCTTCCTGTTGCTTTTTCTGTTCAGCCAAGAACCTTTGTCTTTCCAGCTCTTTTTGCTGTTCCAGTTCTTTCTGACGGGCAATTTCCTGTTGACGTTGTTGCTCTTTTAACACTTCCCGTTGTTTTTCTTCTTCCCGTTTCTGCTCTTCACGTTTTTGGTCTTCAAGGGCTTGTTTCTTTTGTCTGTCCGCCTGACCTTTTTTCTGTTGTTGAATTCGCCCCCAt               >  1:533669/1‑251 (MQ=255)                                                                                                                                                                                                                                  tCCAAGGCTTCTAAGCGAGCTTTTTCTTCCTGTTGCTTTTTCTGTTCAGCCAAGAACCTTTGTCTTTCCAGCTCTTTTTGCTGTTCCAGTTCTTTCTGACGGGCAATTTCCTGTTGACGTTGTTGCTCTTTTAACACTTCCCGTTGTTTTTCTTCTTCCCGTTTCTGCTCTTCACGTTTTTGGTCTTCAAGGGCTTGTTTCTTTTGTCTGTCCGCCTGACCTTTTTTCTGTTGTTGAATTCGCCCCCAtt              >  1:857014/1‑250 (MQ=255)                                                                                                                                                                                                                                        gCTTCTAAGCGAGCTTTTTCTTCCTGTTGCTTTTTCTGTTCAGCCa                                                                                                                                                                                                                    <  1:202535/46‑1 (MQ=255)                                                                                                                                                                                                                                        gCTTCTAAGCGAGCTTTTTCTTCCTGTTGCTTTTTCTGTTCAGCCAAGAACCTTTGTCTTTTCAGCTCTTTTTGCTGTTCCAGTTCTTTCTGACGGGCAATTTCCTGTTGACGTTGTTGCTCTTTTAACACTTCCCGTTGTTTTTCTTCTTCCCGTTTCTGCTCTTCACGTTTTTGGTCTTCAAGGGCTTGTTTCTTTTGTCTGT‑CGCCTGatggtcagtccatcgtgttc                          <  1:426943/231‑20 (MQ=255)                                                                                                                                                                                                                                        gCTTCTAAGCGAGCTTTTTCTTCCTGTTGCTTTTTCTGTTCAGCCAAGAACCTTTGTCTTTCCAGCTCTTTTTGCTGTTCCAGTTCTTTCTGACGGGCAATTTCCTGTTGACGTTGTTGCTCTTTTAACACTTCCCGTTGTTTTTCTTCTTCCCGTTTCTGCTCTTCACGTTTTTGGTCTTCAAggg                                                                       >  1:660260/1‑187 (MQ=255)                                                                                                                                                                                                                                        gCTTCTAAGCGAGCTTTTTCTTCCTGTTGCTTTTTCTGTTCAGCCAAGAACCTTTGTCTTTCCAGCTCTTTTTGCTGTTCCAGTTCTTTCTGACGGGCAATTTCCTGTTGACGTTGTTGCTCTTTTAACACTTCCCGTTGTTTTTCTTCTTCCCGTTTCTGCTCTTCACGTTTTTGGTCTTCAAGGGCTTGTTTCTTTTGTCTGTCCGCCTGACCTTTTTTCTGTTGTTGAATTCGCCCCCATTCCTgcg        >  1:46864/1‑250 (MQ=255)                                                                                                                                                                                                                                        gCTTCTAAGCGAGCTTTTTCTTCCTGTTGCTTTTTCTGTTCAGCCAAGAACCTTTGTCTTTCCAGCTCTTTTTGCTGTTCCAGTTCTTTCTGACGGGCAATTTCCTGTTGACGTTGTTGCTCTTTTAACACTTCCCGTTGTTTTTCTTCTTCCCGTTTCTGCTCTTCACGTTTTTGGTCTTCAAGGGCTTGTTTCTTTTGTCTGTCCGCCTGACCTTTTTTCTGTTGTTGAATTCGCCCCCATTCCTgcg        >  1:143933/1‑250 (MQ=255)                                                                                                                                                                                                                                        gCTTCTAAGCGAGCTTTTTCTTCCTGTTGCTTTTTCTGTTCAGCCAAGAACCTTTGTCTTTCCAGCTCTTTTTGCTGTTCCAGTTCTTTCTGACGGGCAATTTCCTGTTGACGTTGTTGCTCTTTTAACACTTCCCGTTGTTTTTCTTCTTCCCGTTTCTGCTCTTCACGTTTTTGGTCTTCAAGGGCTTGTTTCTTTTGTCTGTCCGCCTGACCTTTTTTCTGTTGTTGAATTCGCCCCCATTCCTgc         <  1:779229/249‑1 (MQ=255)                                                                                                                                                                                                                                         cTTCTAAGCGAGCTTTTTCTTCCTGTTGCTTTTTCTGTTCAGCCAAGAACCTTTGTCTTTCCAGCTCTTTTTGCTGTTCCAGTTCTTTCTGACGGGCAATTTCCTGTTGACGTTGTTGCTCTTTTAACACTTCCCGTTGTTTTTCTTCTTCCCGTTTCTGCTCTTCACGTTTTTGGTCTTCAAGGGCTTGTTTCTTTTGTCTGTCCGCCTGACCTTTTTTCTGTTGTTGAATTCGCCCCCATTCCTgcgc       <  1:548933/250‑1 (MQ=255)                                                                                                                                                                                                                                         cTTCTAAGCGAGCTTTTTCTTCCTGTTGCTTTTTCTGTTCAGCCAAGAACCTTTGTCTTTCCAGCTCTTTTTGCTGTTCCAGTTCTTTCTGACGGGCAATTTCCTGTTGACGTTGTTGCTCTTTTAACACTTCCCGTTGTTTTTCTTCTTCCCGTTTCTGCTCTTCACGTTTTTGGTCTTCAAGGGCTTGTTTCTTTTGTCTGTCCGCCTGACCTTTTTTCTGTTGTTGAATTCGCCCCCATTCCTgcgc       <  1:750966/250‑1 (MQ=255)                                                                                                                                                                                                                                          ttCTAAGCGAGCTTTTTCTTCCTGTTGCTTTTTCTGTTCAGCCAAGAACCTTTGTCTTTCCAGCTCTTTTTGCTGTTCCAGTTCTTTCTGACGGGCAATTTCCTGTTGACGTTGTTGCTCTTTTAACACTTCCCGTTGTTTTTCTTCTTCCCGTTTCTGCTCTTCACGTTTTTGGTCTTCAAGGGCTTGTTTCTTTTGTCTGTCCGCCTGACCTTTTTTCTGTTGTTGAATTCGCCCCCATTcc            <  1:468278/244‑1 (MQ=255)                                                                                                                                                                                                                                             tAAGCGAGCTTTTTCTTCCTGTTGCTTTTTCTGTTCAGCCAAGAACCTTTGTCTTTCCAGCTCTTTTTGCTGTTCCAGTTCTTTCTGACGGGCAATTTCCTGTTGACGTTGTTGCTCTTTTAACACTTCCCGTTGTTTTTCTTCTTCCCGTTTCTGCTCTTCACGTTTTTGGTCTTCAAGGGCTTGTTTCTTTTGTCTGTCCGCCTGACCTTTTTTCTGTTGTTGAATTCGCCCCCATTCCTGCGCTGCCg  <  1:641273/251‑1 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                     GCTTCCGCCTTCGCTTTAGCCTGCGCCTCTGCTTTTGCCTTAGCTTCCGCCTCTGCTTTGGCTTTTGCCTCTTGTTTTGCTTTCTCGTCTGCCTGCACTTTGGCTTTTGCTTCTTCTTCCGCTTGTTTTGCAGCATCTGCTAAACGTTTTGCCTCAGCTTCAGCTTTGAGCTTAGCGGCTTCAGCAGCCTGTTTGGCTTTCGCCTCTTCTGCCTGTTTTTGCTTTTCCAAGGCTTCTAAGCGGGCTTTTTCTTCCTGTTGCTTTTTCTGTTCAGCCAAGAACCTTTGTCTTTCCAGCTCTTTTTGCTGTTCCAGTTCTTTCTGACGGGCAATTTCCTGTTGACGTTGTTGCTCTTTTAACACTTCCCGTTGTTTTTCTTCTTCCCGTTTCTGCTCTTCACGTTTTTGGTCTTCAAGGGCTTGTTTCTTTTGTCTGTCCGCCTGACCTTTTTTCTGTTGTTGAATTCGCCCCCATTCCTGCGCTGCCG  >  1/1996159‑1996645

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑