Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,995,750	G→A	V357V (GTC→GTT)	unknown ←	TolA protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,995,750	0	G	A	100.0%	249.2 / NA	84	V357V (GTC→GTT)	unknown	TolA protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (28/56);  total (28/56)

GCTCGGGCTACCATGGCAAACCCTAACAGCATAATAAATAAGCCAAAAATTCGAATAACTACTTTCATTTTTTCTCCTATTGTTTAAAAGTGCGGTGAAAATCTACCGCACTTTTCTTGGGTCAAAGGTTACAATGGACCTTTTAAATATTTCATATTCTTCCGGCGTAGGCGGTTCGTTCATTTTTCTGGTTTGACCAATAGCATTTAAAGCCGCCCCACATAAATCATTTGGCCCGGAGACCTTTTGGTAAGATAAAATAGTGCCATCACTAGAAAGCTCAATTCTAATATCACAGACCTTACCAAAAAAACGACTATCACCACGAAAATATGGCTTCACAGATTTATAGATAATACCTGCATACCTCGAACCCGCCTTACCGCCATCGCCTGCGCCCGCTGAAGCACCTGAACCCTGGCTGCCTTGTTTATTTATGTTACTACCTTGCAAAGCACTGCCGCCGCC  >  1/1995510‑1995977                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                    gCTCGGGCTACCATGGCAAACCCTAACAGCATAATAAATAAGCCAAAAATTCGAATAACTACTTTCATTTTTTCTCCTATTGTTTAAAAGTGCGGTGAAAATCTACCGCACTTTTCTTGGGTCAAAGGTTACAATGGACCTTTTAAATATTTCATATTCTTCCGGCGTAGGCGGTTCGTTCATTTTTCTGGTTTGACCAATAGCATTTAAAGCCGCCCCACATAAATCATTTGGCCCGGAAACCTTTTGGt                                                                                                                                                                                                                           >  1:713411/1‑251 (MQ=255)             aTGGCAAACCCTAACAGCATAATAAATAAGCCAAAAATTCGAATAACTACTTTCATTTTTTCTCCTATTGTTTAAAAGTGCGGTGAAAATCTACCGCACTTTTCTTGGGTCAAAGGTTACAATGGACCTTTTAAATATTTCATATTCTTCCGGCGTAGGCGGTTCGTTCATTTTTCTGGTTTGACCAATAGCATTTAAAGCCGCCCCACATAAATCATTTGGCCCGGAAACCTTTTGGTAAGATAAAATAg                                                                                                                                                                                                               <  1:474256/251‑1 (MQ=255)             aTGGCAAACCCTAACAGCATAATAAATAAGCCAAAAATTCGAATAACTACTTTCATTTTTTCTCCTATTGTTTAAAAGTGCGGTGAAAATCTACCGCACTTTTCTTGGGTCAAAGGTTACAATGGACCTTTTAAATATTTCATATTCTTCCGGCGTAGGCGGTTCGTTCATTTTTCTGGTTTGACCAATAGCATTTAAAGCCGCCCCACATAAATCATTTGGCCCGGAAACCTTTTGGTAAGATAAAATAg                                                                                                                                                                                                               <  1:691051/251‑1 (MQ=255)             aTGGCAAACCCTAACAGCATAATAAATAAGCCAAAAATTCGAATAACTACTTTCATTTTTTCTCCTATTGTTTAAAAGTGCGGTGAAAATCTACCGCACTTTTCTTGGGTCAAAGGTTACAATGGACCTTTTAAATATTTCATATTCTTCCGGCGTAGGCGGTTCGTTCATTTTTCTGGTTTGACCAATAGCATTTAAAGCCGCCCCACATAAATCATTTGGCCCGGAAACCTTTTGGTAAGATAAAATAg                                                                                                                                                                                                               <  1:737617/251‑1 (MQ=255)             aTGGCAAACCCTAACAGCATAATAAATAAGCCAAAAATTCGAATAACTACTTTCATTTTTTCTCCTATTGTTTAAAAGTGCGGTGAAAATCTACCGCACTTTTCTTGGGTCAAAGGTTACAATGGACCTTTTAAATATTTCATATTCTTCCGGCGTAGGCGGTTCGTTCATTTTTCTGGTTTGACCAATAGCATTTAAAGCCGCCCCACATAAATCATTTGGCCCGGAAACCTTTTGGTAAGATAAAATAg                                                                                                                                                                                                               <  1:216118/251‑1 (MQ=255)                     cccTAACAGCATAATAAATAAGCCAAAAATTCGAATAACTACTTTCATTTTTTCTCCTATTGTTTAAAAGTGCGGTGAAAATCTACCGCACTTTTCTTGGGTCAAAGGTTACAATGGACCTTTTAAATATTTCATATTCTTCCGGCGTAGGCGGTTCGTTCATTTTTCTGGTTTGACCAATAGCATTTAAAGCCGCCCCACATAAATCATTTGGCCCGGAAACCTTTTGGTAAGATAAAATAGTGCCATCa                                                                                                                                                                                                       >  1:164041/1‑251 (MQ=255)                      ccTAACAGCATAATAAATAAGCCAAAAATTCGAATAACTACTTTCATTTTTTCTCCTATTGTTTAAAAGTGCGGTGAAAATCTACCGCACTTTTCTTGGGTCAAAGGTTACAATGGACCTTTTAAATATTTCATATTCTTCCGGCGTAGGCGGTTCGTTCATTTTTCTGGTTTGACCAATAGCATTTAAAGCCGCCCCACATAAATCATTTGGCCCGGAAACCTTTTGGTAAGATAAAATAGTGCCATCAc                                                                                                                                                                                                      <  1:685044/251‑1 (MQ=255)                           cAGCATAATAAATAAGCCAAAAATTCGAATAACTACTTTCATTTTTTCTCCTATTGTTTAAAAGTGCGGTGAAAATCTACCGCACTTTTCTTGGGTCAAAGGTTACAATGGACCTTTTAAATATTTCATATTCTTCCGGCGTAGGCGGTTCGTTCATTTTTCTGGTTTGACCAATAGCATTTAAAGCCGCCCCACATAAATCATTTGGCCCGGAAACCTTTTGGTAAGATAAAATAGTGCCATCACTAGaa                                                                                                                                                                                                 >  1:97807/1‑251 (MQ=255)                             gCATAATAAATAAGCCAAAAATTCGAATAACTACTTTCATTTTTTCTCCTATTGTTTAAAAGTGCGGTGAAAATCTACCGCACTTTTCTTGGGTCAAAGGTTACAATGGACCTTTTAAATATTTCATATTCTTCCGGCGTAGGCGGTTCGTTCATTTTTCTGGTTTGACCAATAGCATTTAAAGCCGCCCCACATAAATCATTTGGCCCGGAAACCTTTTGGTAAGATAAAATAGTGCCATCACTAGaa                                                                                                                                                                                                 <  1:105287/249‑1 (MQ=255)                             gCATAATAAATAAGCCAAAAATTCGAATAACTACTTTCATTTTTTCTCCTATTGTTTAAAAGTGCGGTGAAAATCTACCGCACTTTTCTTGGGTCAAAGGTTACAATGGACCTTTTAAATATTTCATATTCTTCCGGCGTAGGCGGTTCGTTCATTTTTCTGGTTTGACCAATAGCATTTAAAGCCGCCCCACATAAATCATTTGGCCCGGAAACCTTTTGGTAAGATAAAATAGTGCCATCACTAGAAAg                                                                                                                                                                                               >  1:261961/1‑251 (MQ=255)                             gCATAATAAATAAGCCAAAAATTCGAATAACTACTTTCATTTTTTCTCCTATTGTTTAAAAGTGCGGTGAAAATCTACCGCACTTTTCTTGGGTCAAAGGTTACAATGGACCTTTTAAATATTTCATATTCTTCCGGCGTAGGCGGTTCGTTCATTTTTCTGGTTTGACCAATAGCATTTAAAGCCGCCCCACATAAATCATTTGGCCCAGAAACCTTTTGGTAAGATAAAATAGTGCCATCACTAGaaa                                                                                                                                                                                                >  1:281845/1‑250 (MQ=255)                              cATAATAAATAAGCCAAAAATTCGAATAACTACTTTCATTTTTTCTCCTATTGTTTAAAAGTGCGGTGAAAATCTACCGCACTTTTCTTGGGTCAAAGGTTACAATGGACCTTTTAAATATTTCATATTCTTCCGGCGTAGGCGGTTCGTTCATTTTTCTGGTTTGACCAATAGCATTTAAAGCCGCCCCACATAAATCATTTGGCCCGGAAACCTTTTGGTAAGATAAAAt                                                                                                                                                                                                                 >  1:245745/1‑232 (MQ=255)                                          gCCAAAAATTCGAATAACTACTTTCATTTTTTCTCCTATTGTTTAAAAGTGCGGTGAAAATCTACCGCACTTTTCTTGGGTCAAAGGTTACAATGGACCTTTTAAATATTTCATATTCTTCCGGCGTAGGCGGTTCGTTCATTTTTCTGGTTTGACCAATAGCATTTAAAGCCGCCCCACATAAATCATTTGGCCCGGAAACCTTTTGGTAAGATAAAATAGTGCCATCACTAGAAAGCTCAATTCTAata                                                                                                                                                                                  >  1:237052/1‑251 (MQ=255)                                                   tCGAATAACTACTTTCATTTTTTCTCCTATTGTTTAAAAGTGCGGTGAAAATCTACCGCACTTTTCTTGGGTCAAAGGTTACAATGGACCTTTTAAATATTTCATATTCTTCCGGCGTAGGCGGTTCGTTCATTTTTCTGGTTTGACCAATAGCATTTAAAGCCGCCCCACATAAATCATTTGGCCCGGAAACCTTTTGGTAAGATAAAATAGTGCCATCACTAGAAAGCTCAATTCTAATATCACAGAcc                                                                                                                                                                         <  1:591352/251‑1 (MQ=255)                                                   tCGAATAACTACTTTCATTTTTTCTCCTATTGTTTAAAAGTGCGGTGAAAATCTACCGCACTTTTCTTGGGTCAAAGGTTACAATGGACCTTTTAAATATTTCATATTCTTCCGGCGTAGGCGGTTCGTTCATTTTTCTGGTTTGACCAATAGCATTTAAAGCCGCCCCACATAAATCATTTGGCCCGGAAACCTTTTGGTAAGATAAAATAGTGCCATCACTAGAAAGCTCAATTCTAATATCACAGAcc                                                                                                                                                                         <  1:582880/251‑1 (MQ=255)                                                   tCGAATAACTACTTTCATTTTTTCTCCTATTGTTTAAAAGTGCGGTGAAAATCTACCGCACTTTTCTTGGGTCAAAGGTTACAATGGACCTTTTAAATATTTCATATTCTTCCGGCGTAGGCGGTTCGTTCATTTTTCTGGTTTGACCAATAGCATTTAAAGCCGCCCCACATAAATCATTTGGCCCGGAAACCTTTTGGTAAGATAAAATAGTGCCATCACTAGAAAGCTCAATTCTAATATCACAGAcc                                                                                                                                                                         <  1:647119/251‑1 (MQ=255)                                                   tCGAATAACTACTTTCATTTTTTCTCCTATTGTTTAAAAGTGCGGTGAAAATCTACCGCACTTTTCTTGGGTCAAAGGTTACAATGGACCTTTTAAATATTTCATATTCTTCCGGCGTAGGCGGTTCGTTCATTTTTCTGGTTTGACCAATAGCATTTAAAGCCGCCCCACATAAATCATTTGGCCCGGAAACCTTTTGGTAAGATAAAATAGTGCCATCACTAGAAAGCTCAATTCTAATATCACAGAcc                                                                                                                                                                         <  1:681719/251‑1 (MQ=255)                                                   tCGAATAACTACTTTCATTTTTTCTCCTATTGTTTAAAAGTGCGGTGAAAATCTACCGCACTTTTCTTGGGTCAAAGGTTACAATGGACCTTTTAAATATTTCATATTCTTCCGGCGTAGGCGGTTCGTTCATTTTTCTGGTTTGACCAATAGCATTTAAAGCCGCCCCACATAAATCATTTGGCCCGGAAACCTTTTGGTAAGATAAAATAGTGCCATCACTAGAAAGCTCAATTCTAATATCACAGAcc                                                                                                                                                                         <  1:117030/251‑1 (MQ=255)                                                   tCGAATAACTACTTTCATTTTTTCTCCTATTGTTTAAAAGTGCGGTGAAAATCTACCGCACTTTTCTTGGGTCAAAGGTTACAATGGACCTTTTAAATATTTCATATTCTTCCGGCGTAGGCGGTTCGTTCATTTTTCTGGTTTGACCAATAGCATTTAAAGCCGCCCCACATAAATCATTTGGCCCGGAAACCTTTTGGTAAGATAAAATAGTGCCATCACTAGAAAGCTCAATTCTAATATCACAGAcc                                                                                                                                                                         <  1:314085/251‑1 (MQ=255)                                                             actTTCATTTTTTCTCCTATTGTTTAAAAGTGCGGTGAAAATCTACCGCACTTTTCTTGGGTCAAAGGTTACAATGGACCTTTTAAATATTTCATATTCTTCCGGCGTAGGCGGTTCGTTCATTTTTCTGGTTTGACCAATAGCATTTAAAGCCGCCCCACATAAATCATTTGGCCCGGAAACCTTTTGGTAAGATAAAATAGTGCCATCACTAGAAAGCTCAATTCTAATATCACAGACCTTACCaaaaa                                                                                                                                                               <  1:208542/251‑1 (MQ=255)                                                                        ttCTCCTATTGTTTAAAAGTGCGGTGAAAATCTACCGCACTTTTCTTGGGTCAAAGGTTACAATGGACCTTTTAAATATTTCATATTCTTCCGGCGTAGGCGGTTCGTTCATTTTTCTGGTTTGACCAATAGCATTTAAAGCCGCCCCACATAAATCATTTGGCCCGGAAACCTTTTGGTAAGATAAAATAGTGCCATCACTAGAAAGCTCAATTCTAATATCACAGACCTTACCAAAAAAACGACTATca                                                                                                                                                    <  1:385143/251‑1 (MQ=255)                                                                                  gTTTAAAAGTGCGGTGAAAATCTACCGCACTTTTCTTGGGTCAAAGGTTACAATGGACCTTTTAAATATTTCATATTCTTCCGGCGTAGGCGGTTCGTTCATTTTTCTGGTTTGACCAATAGCATTTAAAGCCGCCCCACATAAATCATTTGGCCCGGAAACCTTTTGGTAAGATAAAAt                                                                                                                                                                                                                 <  1:544364/180‑1 (MQ=255)                                                                                  gTTTAAAAGTGCGGTGAAAATCTACCGCACTTTTCTTGGGTCAAAGGTTACAATGGACCTTTTAAATATTTCATATTCTTCCGGCGTAGGCGGTTCGTTCATTTTTCTGGTTTGACCAATAGCATTTAAAGCCGCCCCACATAAATCATTTGGCCCGGAAACCTTTTGGTAAGATAAAATAGTGCCATCACTAGaa                                                                                                                                                                                                 <  1:605712/196‑1 (MQ=255)                                                                                  gTTTAAAAGTGCGGTGAAAATCTACCGCACTTTTCTTGGGTCAAAGGTTACAATGGACCTTTTAAATATTTCATATTCTTCCGGCGTAGGCGGTTCGTTCATTTTTCTGGTTTGACCAATAGCATTTAAAGCCGCCCCACATAAATCATTTGGCCCGGAAACCTTTTGGTAAGATAAAATAGTGCCATCACTAGAAAGCTCAATTCTAATATCACAGAcc                                                                                                                                                                         <  1:41304/220‑1 (MQ=255)                                                                                  gTTTAAAAGTGCGGTGAAAATCTACCGCACTTTTCTTGGGTCAAAGGTTACAATGGACCTTTTAAATATTTCATATTCTTCCGGCGTAGGCGGTTCGTTCATTTTTCTGGTTTGACCAATAGCATTTAAAGCCGCCCCACATAAATCATTTGGCCCGGAAACCTTTTGGTAAGATAAAATAGTGCCATCACTAGAAAGCTCAATTCTAATATCACAGACCTTACCAAAAAAACGACTATCACCACGAAAat                                                                                                                                          >  1:556243/1‑251 (MQ=255)                                                                                  gTTTAAAAGTGCGGTGAAAATCTACCGCACTTTTCTTGGGTCAAAGGTTACAATGGACCTTTTAAATATTTCATATTCTTCCGGCGTAGGCGGTTCGTTCATTTTTCTGGTTTGACCAATAGCATTTAAAGCCGCCCCACATAAATCATTTGGCCCGGAAACCTTTTGGTAAGATAAAATAGTGCCATCACTAGAAAGCTCAATTCTAATATCACAGACCTTACCAAAAAAACGACTATCACCACGAAAat                                                                                                                                          >  1:812942/1‑251 (MQ=255)                                                                                    ttAAAAGTGCGGTGAAAATCTACCGCACTTTTCTTGGGTCAAAGGTTACAATGGACCTTTTAAATATTTCATATTCTTCCGGCGTAGGCGGTTCGTTCATTTTTCTGGTTTGACCAATAGCATTTAAAGCCGCCCCACATAAATCATTTGGCCCGGAAACCTTTTGGTAAGATAAAATAGTGCCATCACTAGAAAGCTCAATTCTAATATCACAGACCTTACCAAAAAAACGACTATCACCACGAAAatat                                                                                                                                        <  1:672494/251‑1 (MQ=255)                                                                                               gTGAAAATCTACCGCACTTTTCTTGGGTCAAAGGTTACAATGGACCTTTTAAATATTTCATATTCTTCCGGCGTAGGCGGTTCGTTCATTTTTCTGGTTTGACCAATAGCATTTAAAGCCGCCCCACATAAATCATTTGGCCCGGAAACCTTTTGGTAAGATAAAATAGTGCCATCACTAGAAAGCTCAATTCTaa                                                                                                                                                                                    >  1:895287/1‑196 (MQ=255)                                                                                                tGAAAATCTACCGCACTTTTCTTGGGTCAAAGGTTACAATGGACCTTTTAAATATTTCATATTCTTCCGGCGTAGGCGGTTCGTTCATTTTTCTGGTTTGACCAATAGCATTTAAAGCCGCCCCACATAAATCATTTGGCCCGGAAACCTTTTGGTAAGATAAAATAGTGCCATCACTAGAAAGCTCAATTCTAATATCACAGACCTTACCAAAAAAACGACTATCACCACGAAAATATGGCTTCACAGAt                                                                                                                            <  1:47790/251‑1 (MQ=255)                                                                                                    aaTCTACCGCACTTTTCTTGGGTCAAAGGTTACAATGGACCTTTTAAATATTTCATATTCTTCCGGCGTAGGCGGTTCGTTCATTTTTCTGGTTTGACCAATAGCATTTAAAGCCGCCCCACATAAATCATTTGGCCCGGAAACCTTTTGGTAAGATAAAATAGTGCCATCACTAGAAAGCTCAATTCTAATATCACAGACCTTACCAAAAAAACGACTATCACCACGAAAATATGGCTTCACAGATTtat                                                                                                                        <  1:812900/251‑1 (MQ=255)                                                                                                        tACCGCACTTTTCTTGGGTCAAAGGTTACAATGGACCTTTTAAATATTTCATATTCTTCCGGCGTAGGCGGTTCGTTCATTTTTCTGGTTTGACCAATAGCATTTAAAGCCGCCCCACATAAATCATTTGGCCCGGAAACCTTTTGGTAAGATAAAATAGTGCCATCACTAGAAAGCTCAATTCTAATATCACAGTCCTTACCAAAAAAACGACTATCACCACGAAAATATGGCTTCACAGATTTATAGat                                                                                                                    <  1:252819/251‑1 (MQ=255)                                                                                                        tACCGCACTTTTCTTGGGTCAAAGGTTACAATGGACCTTTTAAATATTTCATATTCTTCCGGCGTAGGCGGTTCGTTCATTTTTCTGGTTTGACCAATAGCATTTAAAGCCGCCCCACATAAATCATTTGGCCCGGAAACCTTTTGGTAAGATAAAATAGTGCCATCACTAGAAAGCTCAATTCTAATATCACAGACCTTACCAAAAAAACGACTATCACCACGAAAATATGGCTTCACAGATTTATAGat                                                                                                                    <  1:586468/251‑1 (MQ=255)                                                                                                        tACCGCACTTTTCTTGGGTCAAAGGTTACAATGGACCTTTTAAATATTTCATATTCTTCCGGCGTAGGCGGTTCGTTCATTTTTCTGGTTTGACCAATAGCATTTAAAGCCGCCCCACATAAATCATTTGGCCCGGAAACCTTTTGGTAAGATAAAATAGTGCCATCACTAGAAAGCTCAATTCTAATATCACAGACCTTACCAAAAAAACGACTATCACCACGAAAATATGGCTTCACAGATTTATAGat                                                                                                                    <  1:305715/251‑1 (MQ=255)                                                                                                              aCTTTTCTTGGGTCAAAGGTTACAATGGACCTTTTAAATATTTCATATTCTTCCGGCGTAGGCGGTTCGTTCATTTTTCTGGTTTGACCAATAGCATTTAAAGCCGCCCCACATAAATCATTTGGCCCGGAAACCTTTTGGTAAGATAAAATAGTGCCATCACTAGAAAGCTCAATTCTAATATCACAGACCTTACCAAAAAAACGACTATCACCACGAAAATATGGCTTCACAGATTTATAGATAATAcc                                                                                                              <  1:355494/251‑1 (MQ=255)                                                                                                              aCTTTTCTTGGGTCAAAGGTTACAATGGACCTTTTAAATATTTCATATTCTTCCGGCGTAGGCGGTTCGTTCATTTTTCTGGTTTGACCAATAGCATTTAAAGCCGCCCCACATAAATCATTTGGCCCGGAAACCTTTTGGTAAGATAAAATAGTGCCATCACTAGAAAGCTCAATTCTAATATCACAGACCTTACCAAAAAAACGACTATCACCACGAAAATATGGCTTCACAGATTTATAGATAATAcc                                                                                                              <  1:312864/251‑1 (MQ=255)                                                                                                                 tttCTTGGGTCAAAGGTTACAATGGACCTTTTAAATATTTCATATTCTTCCGGCGTAGGCGGTTCGTTCATTTTTCTGGTTTGACCAATAGCATTTAAAGCCGCCCCACATAAATCATTTGGCCCGGAAACCTTTTGGTAAGATAAAAt                                                                                                                                                                                                                 >  1:741859/1‑149 (MQ=255)                                                                                                                 tttCTTGGGTCAAAGGTTACAATGGACCTTTTAAATATTTCATATTCTTCCGGCGTAGGCGGTTCGTTCATTTTTCTGGTTTGACCAATAGCATTTAAAGCCGCCCCACATAAATCATTTGGCCCGGAAACCTTTTGGTAAGATAAAATAGTGCCATCACTAGAAAGCTCAATTCTAATATcac                                                                                                                                                                              <  1:300436/184‑1 (MQ=255)                                                                                                                 tttCTTGGGTCAAAGGTTACAATGGACCTTTTAAATATTTCATATTCTTCCGGCGTAGGCGGTTCGTTCATTTTTCTGGTTTGACCAATAGCATTTAAAGCCGCCCCACATAAATCATTTGGCCCGGAAACCTTTTGGTAAGATAAAATAGTGCCATCACTAGAAAGCTCAATTCTAATATCACAGAcc                                                                                                                                                                         >  1:548536/1‑189 (MQ=255)                                                                                                                 tttCTTGGGTCAAAGGTTACAATGGACCTTTTAAATATTTCATATTCTTCCGGCGTAGGCGGTTCGTTCATTTTTCTGGTTTGACCAATAGCATTTAAAGCCGCCCCACATAAATCATTTGGCCCGGAAACCTTTTGGTAAGATAAAATAGTGCCATCACTAGAAAGCTCAATTCTAATATCACAGACCTTACCAAAAAAACGACTATCACCACGAAAATATGGCTTCACAGATTTATAg                                                                                                                      <  1:739154/240‑1 (MQ=255)                                                                                                                   tCTTGGGTCAAAGGTTACAATGGACCTTTTAAATATTTCATATTCTTCCGGCGTAGGCGGTTCGTTCATTTTTCTGGTTTGACCAATAGCATTTAAAGCCGCCCCACATAAATCATTTGGCCCGGAAACCTTTTGGTAAGATAAAATAGTGCCATCACTAGAAAGCTCAATTCTAATATCACAGACCTTACCAAAAAAACGACTATCACCACGAAAATATGGCTTCACAGATTTATAGATAATACCTGCAt                                                                                                         <  1:299893/251‑1 (MQ=255)                                                                                                                   tCTTGGGTCAAAGGTTACAATGGACCTTTTAAATATTTCATATTCTTCCGGCGTAGGCGGTTCGTTCATTTTTCTGGTTTGACCAATAGCATTTAAAGCCGCCCCACATAAATCATTTGGCCCGGAAACCTTTTGGTAAGATAAAATAGTGCCATCACTAGAAAGCTCAATTCTAATATCACAGACCTTACCAAAAAAACGACTATCACCACGAAAATATGGCTTCACAGATTTATAGATAATACCTGCAt                                                                                                         <  1:47393/251‑1 (MQ=255)                                                                                                                     ttGGGTCAAAGGTTACAATGGACCTTTTAAATATTTCATATTCTTCCGGCGTAGGCGGTTCGTTCATTTTTCTGGTTTGACCAATAGCATTTAAAGCCGCCCCACATAAATCATTTGGCCCGGAAACCTTTTGGTAAGATAAAATAGTGCCATCACTAGAAAGCTCAATTCTAATATCACAGACCTTACCAAAAAAACGACTATCACCACGAAAATATGGCTTCACAGATTTATAGATAATACCTGCATAc                                                                                                       <  1:261611/251‑1 (MQ=255)                                                                                                                             aaGGTTACAATGGACCTTTTAAATATTTCATATTCTTCCGGCGTAGGCGGTTCGTTCATTTTTCTGGTTTGACCAATAGCATTTAAAGCCGCCCCACATAAATCATTTGGCCCGGAAACCTTTTGGTAAGATAAAATAGTGCCATCACTAGAAAGCTCAATTCTAATATCACAGACCTTACCAAAAAAACGACTATCACCACGAAAATATGGCTTCACAGATTTATAGATAATACCTGCATACCTCGAAcc                                                                                               <  1:391960/251‑1 (MQ=255)                                                                                                                             aaGGTTACAATGGACCTTTTAAATATTTCATATTCTTCCGGCGTAGGCGGTTCGTTCATTTTTCTGGTTTGACCAATAGCATTTAAAGCCGCCCCACATAAATCATTTGGCCCGGAAACCTTTTGGTAAGATAAAATAGTGCCATCACTAGAAAGCTCAATTCTAATATCACAGACCTTACCAAAAAAACGACTATCACCACGAAAATATGGCTTCACAGATTTATAGATAATACCTGCATACCTCGAAcc                                                                                               <  1:841817/251‑1 (MQ=255)                                                                                                                               ggTTACAATGGACCTTTTAAATATTTCATATTCTTCCGGCGTAGGCGGTTCGTTCATTTTTCTGGTTTGACCAATAGCATTTAAAGCCGCCCCACATAAATCATTTGGCCCGGAAACCTTTTGGTAAGTTAAAATAGTGCCATCACTAGAAAGCTCAATTCTTATATCACAGACCTTACCAAAAAAACGTCTATCACCACGAAAATATGGCTTCACAGATTTATAGATAATACCTGCATACCTCGAACCCg                                                                                             >  1:840716/1‑251 (MQ=255)                                                                                                                               ggTTACAATGGACCTTTTAAATATTTCATATTCTTCCGGCGTAGGCGGTTCGTTCATTTTTCTGGTTTGACCAATAGCATTTAAAGCCGCCCCACATAAATCATTTGGCCCGGAAACCTTTTGGTAAGATAAAATAGTGCCATCACt                                                                                                                                                                                                     <  1:699/147‑1 (MQ=255)                                                                                                                               ggTTACAATGGACCTTTTAAATATTTCATATTCTTCCGGCGTAGGCGGTTCGTTCATTTTTCTGGTTTGACCAATAGCATTTAAAGCCGCCCCACATAAATCATTTGGCCCGGAAACCTTTTGGTAAGATAAAATAGTGCCATCACTAGAAAGCTCAATTCTAATATCACAGAc                                                                                                                                                                          <  1:634890/174‑1 (MQ=255)                                                                                                                               ggTTACAATGGACCTTTTAAATATTTCATATTCTTCCGGCGTAGGCGGTTCGTTCATTTTTCTGGTTTGACCAATAGCATTTAAAGCCGCCCCACATAAATCATTTGGCCCGGAAACCTTTTGGTAAGATAAAATAGTGCCATCACTAGAAAGCTCAATTCTAATATCACAGACCTTACCAAAAAAACGACTATCACCACGAAAATATGGCTTCACAGATTTATAGATAATAcc                                                                                                              <  1:460140/234‑1 (MQ=255)                                                                                                                               ggTTACAATGGACCTTTTAAATATTTCATATTCTTCCGGCGTAGGCGGTTCGTTCATTTTTCTGGTTTGACCAATAGCATTTAAAGCCGCCCCACATAAATCATTTGGCCCGGAAACCTTTTGGTAAGATAAAATAGTGCCATCACTAGAAAGCTCAATTCTAATATCACAGACCTTACCAAAAAAACGACTATCACCACGAAAATATGGCTTCACAGATTTATAGATAATACCTGCATAc                                                                                                       >  1:797755/1‑241 (MQ=255)                                                                                                                               ggTTACAATGGACCTTTTAAATATTTCATATTCTTCCGGCGTAGGCGGTTCGTTCATTTTTCTGGTTTGACCAATAGCATTTAAAGCCGCCCCACATAAATCATTTGGCCCGGAAACCTTTTGGTAAGATAAAATAGTGCCATCACTAGAAAGCTCAATTCTAATATCACAGACCTTACCAAAAAAACGACTATCACCACGAAAATATGGCTTCACAGATTTATAGATAATACCTGCATACCTCGAACCCg                                                                                             >  1:623924/1‑251 (MQ=255)                                                                                                                                gTTACAATGGACCTTTTAAATATTTCATATTCTTCCGGCGTAGGCGGTTCGTTCATTTTTCTGGTTTGACCAATAGCATTTAAAGCCGCCCCACATAAATCATTTGGCCCGGAAACCTTTTGGTAAGATAAAATAGTGCCATCACTAGAAAGCTCAATTCTAATATCACAGACCTTACCAAAAAAACGACTATCACCACGAAAATATGGCTTCACAGATTTATAGATAATAcc                                                                                                              <  1:67595/233‑1 (MQ=255)                                                                                                                                 ttACAATGGACCTTTTAAATATTTCATATTCTTCCGGCGTAGGCGGTTCGTTCATTTTTCTGGTTTGACCAATAGCATTTAAAGCCGCCCCACATAAATCATTTTTCCCGGAAACCTTTTGGTAAGATAAAATAGGGCCATCACCAGAAAGCCCAATTCTAATATCACAGACCTTACCAAAAAAACGACTATCACCACGAAAATATGGCTTCACAGATTtat                                                                                                                        >  1:10574/1‑222 (MQ=255)                                                                                                                                   aCAATGGACCTTTTAAATATTTCATATTCTTCCGGCGTAGGCGGTTCGTTCATTTTTCTGGTTTGACCAATAGCATTTAAAGCCGCCCCACATAAATCATTTGGCCCGGAAACCTTTTGGTAAGATAAAATAGTGCCATCACTAGAAAGCTCAATTCTAata                                                                                                                                                                                  <  1:842454/162‑1 (MQ=255)                                                                                                                                   aCAATGGACCTTTTAAATATTTCATATTCTTCCGGCGTAGGCGGTTCGTTCATTTTTCTGGTTTGACCAATAGCATTTAAAGCCGCCCCACATAAATCATTTGGCCCGGAAACCTTTTGGTAAGATAAAATAGTGCCATCACTAGAAAGCTCAATTCTAATATCACAGACCTTACCAAAAAAACGACTATCACCACGAAAATATGGCTTCACAGATTTATAGat                                                                                                                    >  1:670763/1‑224 (MQ=255)                                                                                                                                   aCAATGGACCTTTTAAATATTTCATATTCTTCCGGCGTAGGCGGTTCGTTCATTTTTCTGGTTTGACCAATAGCATTTAAAGCCGCCCCACATAAATCATTTGGCCCGGAAACCTTTTGGTAAGATAAAATAGTGCCATCACTAGAAAGCTCAATTCTAATATCACAGACCTTACCAAAAAAACGACTATCACCACGAAAATATGGCTTCACAGATTTATAGATAATACCTGCATACCTc                                                                                                    <  1:841724/240‑1 (MQ=255)                                                                                                                                           ccTTTTAAATATTTCATATTCTTCCGGCGTAGGCGGTTCGTTCATTTTTCTGGTTTGACCAATAGCATTTAAAGCCGCCCCACATAAATCATTTGGCCCGGAAACCTTTTGGTAAGATAAAATAGTGCCATCACt                                                                                                                                                                                                     <  1:864208/135‑1 (MQ=255)                                                                                                                                             ttttAAATATTTCATATTCTTCCGGCGTAGGCGGTTCGTTCATTTTTCTGGTTTGACCAATAGCATTTAAAGCCGCCCCACATAAATCATTTGGCCCGGAAACCTTTTGGTAAGATAAAATAGTGCCATCACTAGAAAGCTCAATTCTAATATCACAGACCTTACCAAAAAAACGACTATCACCACGAAAATATGGCTTCACAGATTTATAGATAATAcc                                                                                                              <  1:458214/220‑1 (MQ=255)                                                                                                                                                     atTTCATATTCTTCCGGCGTAGGCGGTTCGTTCATTTTTCTGGTTTGACCAATAGCATTTAAAGCCGCCCCACATAAATCATTTGGCCCGGAAACCTTTTGGTAAGATAAAATAGTGCCATCACTAGAAAGCTCAATTCTAata                                                                                                                                                                                  >  1:160809/1‑144 (MQ=255)                                                                                                                                                     atTTCATATTCTTCCGGCGTAGGCGGTTCGTTCATTTTTCTGGTTTGACCAATAGCATTTAAAGCCGCCCCACATAAATCATTTGGCCCGGAAACCTTTTGGTAAGATAAAATAGTGCCATCACTAGAAAGCTCAATTCTAATATCACAGACCTTACCAAAAAAACGACTATCACCACGAAAATATGGCTTCACAGATTTATAGATAATACCTGCATACCTCGAACCCGCCTTACCGCCATCGCCTgcgc                                                                        >  1:27965/1‑250 (MQ=255)                                                                                                                                                      tttCATATTCTTCCGGCGTAGGCGGTTCGTTCATTTTTCTGGTTTGACCAATAGCATTTAAAGCCGCCCCACATAAATCATTTGGCCCGGAAACCTTTTGGTAAGATAAAATAGTGCCATCACTAGAAAGCTCAATTCTAATATcac                                                                                                                                                                              <  1:750853/147‑1 (MQ=255)                                                                                                                                                          atatTCTTCCGGCGTAGGCGGTTCGTTCATTTTTCTGGTTTGACCAATAGCATTTAAAGCCGCCCCACATAAATCATTTGGCCCGGAAACCTTTTGGTAAGATAAAATAGTGCCATCACTAGAAAGCTCAATTCTAATATCACAGACCTTACCaa                                                                                                                                                                  <  1:138414/155‑1 (MQ=255)                                                                                                                                                           tatTCTTCCGGCGTAGGCGGTTCGTTCATTTTTCTGGTTTGACCAATAGCATTTAAAGCCGCCCCACATAAATCATTTGGCCCGGAAACCTTTTGGTAAGATAAAATAGTGCCATCACTAGAAAGCTCAATTCTAATATCACAGACCTTACCAAAAAAACGACTATCACCACGAAAATATGGCTTCACAGATTTATAGATAATACCTGCATAc                                                                                                       <  1:410936/213‑1 (MQ=255)                                                                                                                                                              tcttcCGGCGTTGGCGGTTCTTTCATTTTTCTGTTTTGGCCAATAGCATTTAAAGCCGCCCCTCATAAAACATTTGGCCCGGAAACCTTTTGGTAAGATAAAATAGTGCCATCACTATAAAGCACAATTCTAATATCACAGACCTTACCAAAAAAACGACTATCACCACGAAAATATGGCTTCACAGATTTATAGATAATACCTGCATACCTCGAACCCGCCTTACCGCCATCGCCTGCGCCCGCTGAAGc                                                              <  1:514760/251‑1 (MQ=255)                                                                                                                                                               cttcCGGCGTAGGCGGTTCGTTCATTTTTCTGGTTTGACCAATAGCATTTAAAGCCGCCCCACATAAATCATTTGGCCCGGAAACCTTTTGGTAAGATAAAATAGTGCCATCACTAGAAAGCTCAATTCTAATATCACAGACCTTACCAAAAAAACGACTATCACCACGAAAATATGGCTTCACAGATTTATAGATAATACCTGCATAcc                                                                                                      <  1:333056/210‑1 (MQ=255)                                                                                                                                                                    ggCGTAGGCGGTTCGTTCATTTTTCTGGTTTGACCAATAGCATTTAAAGCCGCCCCACATAAATCATTTGGCCCGGAAACCTTTTGGTAAGATAAAATAGTGCCATCACTAGAAAGCTCAATTCTAata                                                                                                                                                                                  <  1:582844/129‑1 (MQ=255)                                                                                                                                                                    ggCGTAGGCGGTTCGTTCATTTTTCTGGTTTGACCAATAGCATTTAAAGCCGCCCCACATAAATCATTTGGCCCGGAAACCTTTTGGTAAGATAAAATAGTGCCATCACTAGAAAGCTCAATTCTAATATCACAGACCTTACCAAAAAAACGACTATCACCACGAAAatat                                                                                                                                        >  1:688781/1‑171 (MQ=255)                                                                                                                                                                     gCGTAGGCGGTTCGTTCATTTTTCTGGTGTGACCAATAGCATTTAAAGCCGCCCCACATAAATCATTTGGCCCGGAAACCTTTTGGTAAGATAAAATAGTGCCATCACTAGAAAGCTCAATTCTAATATCACAGACCTTACCAAAAAAACGACTATCACCACGAAAATATGGCTTCACAGATTTATAGATAATACCTg                                                                                                            <  1:452016/198‑1 (MQ=255)                                                                                                                                                                        tAGGCGGTTCGTTCATTTTTCTGGTTTGACCAATAGCATTTAAAGCCGCCCCACATAAATCATTTGGCCCGGAAACCTTTTGGTAAGATAAAATAGTGCCATCACTAGAAAGCTCAATTCTAATATCACAGACCTTACCAAAAAAACGACTATCACCACGAAAATATGGCTTCACAGATTTATAGATAATACCTGCATACCTCGAACCCGCCTTACCGCCATCGCCTGCGCCCGCTGAAGCACCTGAAccc                                                    <  1:339249/251‑1 (MQ=255)                                                                                                                                                                                       tttttCTGGTTTGACCAATAGCATTTAAAGCCGCCCCACATAAATCATTTGGCCCGGAAACCTTTTGGTAAGATAAAATAGTGCCATCACTAGAAAGCTCAATTCTAATATCACAGACCTTACCAAAAAAACGACTATCACCACGAAAATATGGCTTCACAGATTTATAGATAATACCTGCATACCTCGAACCCGCCTTACCGCCATCGCCTGCGCCCGCTGAAGc                                                              >  1:289138/1‑226 (MQ=255)                                                                                                                                                                                         tttCTGGTTTGACCAATAGCATTTAAAGCCGCCCCACATAAATCATTTGGCCCGGAAACCTTTTGGTAAGATAAAATAGTGCCATCACTAGAAAGCTCAATTCTAATATCACAGACCTTACCAAAAAAACGACTATCACCACGAAAATATGGCTTCACAGATTTATAGATAATACCTGCATACCTCGAACCCGCCTTACCGCCATCGCCTGCGCCCGCTGAAGCACCTGAACCCTGGCTGCCTTGtttatt                                   >  1:26479/1‑251 (MQ=255)                                                                                                                                                                                           tCTGGTTTGACCAATAGCATTTAAAGCCGCCCCACATAAATCATTTGGCCCGGAAACCTTTTGGTAAGATAAAATAGTGCCATCACTAGAAAGCTCAATTCTAATATCACAGACCTTACCAAAAAAACGACTATCACCACGAAAATATGGCTTCACAGATTTATAGATAATACCTGCATACCTCGAACCCGCCTTACCGCCATCGCCTGCGCCCGCTGAAGCACCTGAACCCTGGCTGCCTTGtttattta                                 <  1:157678/251‑1 (MQ=255)                                                                                                                                                                                           tCTGGTTTGACCAATAGCATTTAAAGCCGCCCCACATAAATCATTTGGCCCGGAAACCTTTTGGTAAGATAAAATAGTGCCATCACTAGAAAGCTCAATTCTAATATCACAGACCTTACCAAAAAAACGACTATCACCACGAAAATATGGCTTCACAGATTTATAGATAATACCTGCATACCTCGAACCCGCCTTACCGCCATCGCCTGCGCCCGCTGAAGCACCTGAACCCTGGCTGCCTTGtttattta                                 <  1:800207/251‑1 (MQ=255)                                                                                                                                                                                           tCTGGTTTGACCAATAGCATTTAAAGCCGCCCCACATAAATCATTTGGCCCGGAAACCTTTTGGTAAGATAAAATAGTGCCATCACTAGAAAGCTCAATTCTAATATCACAGACCTTACCAAAAAAACGACTATCACCACGAAAATATGGCTTCACAGATTTATAGATAATACCTGCATACCTCGAACCCGCCTTACCGCCATCGCCTGCGCCCGCTGAAGCACCTGAACCCTGGCTGCCTTGtttattta                                 <  1:842754/251‑1 (MQ=255)                                                                                                                                                                                                   gACCAATAGCATTTAAAGCCGCCCCACATAAATCATTTGGCCCGGAAACCTTTTGGTAAGATAAAATAGTGCCATCACTAGAAAGCTCAATTCTAATATCACAGACCTTACCAAAAAAACGACTATCACCACGAAAATATGGCTTCACAGATTTATAGATAATACCTGCATACCTCGAAcc                                                                                               >  1:813854/1‑181 (MQ=255)                                                                                                                                                                                                   gACCAATAGCATTTAAAGCCGCCCCACATAAATCATTTGGCCCGGAAACCTTTTGGTAAGATAAAATAGTGCCATCACTAGAAAGCTCAATTCTAATATCACAGACCTTACCAAAAAAACGACTATCACCACGAAAATATGGCTTCACAGATTTATAGATAATACCTGCATACCTCGAACCCGCCTTACCGCCATCGCCTGCGCCCGCTGAAGCACCTGAACCCTGGCTGCCTTGTTTATTTATGTtacta                         >  1:539962/1‑251 (MQ=255)                                                                                                                                                                                                    aCCAATAGCATTTAAAGCCGCCCCACATAAATCATTTGGCCCGGAAACCTTTTGGTAAGATAAAATAGTGCCATCACTAGAAAGCTCAATTCTAATATCACAGACCTTACCAAAAAAACGACTATCACCACGAAAATATGGCTTCACAGATTTATAGATAATACCTGCATACCTCGAACCCGCCTTACCGCCATCGCCTGCGCCCGCTGAAGCACCTGAACCCTGGCTGCCTTGTTTATTTATGTtactac                        <  1:39210/251‑1 (MQ=255)                                                                                                                                                                                                    aCCAATAGCATTTAAAGCCGCCCCACATAAATCATTTGGCCCGGAAACCTTTTGGTAAGATAAAATAGTGCCATCACTAGAAAGCTCAATTCTAATATCACAGACCTTACCAAAAAAACGACTATCACCACGAAAATATGGCTTCACAGATTTATAGATAATACCTGCATACCTCGAACCCGCCTTACCGCCATCGCCTGCGCCCGCTGAAGCACCTGAACCCTGGCTGCCTTGTTTATTTATGTtactac                        <  1:199316/251‑1 (MQ=255)                                                                                                                                                                                                    aCCAATAGCATTTAAAGCCGCCCCACATAAATCATTTGGCCCGGAAACCTTTTGGTAAGATAAAATAGTGCCATCACTAGAAAGCTCAATTCTAATATCACAGACCTTACCAAAAAAACGACTATCACCACGAAAATATGGCTTCACAGATTTATAGATAATACCTGCATACCTCGAACCCGCCTTACCGCCATCGCCTGCGCCCGCTGAAGCACCTGAACCCTGGCTGCCTTGTTTATTTATGTtactac                        <  1:4903/251‑1 (MQ=255)                                                                                                                                                                                                             aTTTAAAGCCGCCCCACATAAATCATTTGGCCCGGAAACCTTTTGGTAAGATAAAATAGTGCCATCACTAGAAAGCTCAATTCTAATATCACAGACCTTACCAAAAAAACGACTATcac                                                                                                                                                   >  1:59693/1‑119 (MQ=255)                                                                                                                                                                                                             aTTTAAAGCCGCCCCACATAAATCATTTGGCCCGGAAACCTTTTGGTAAGATAAAATAGTGCCATCACTAGAAAGCTCAATTCTAATATCACAGACCTTACCAAAAAAACGACTATCACCACGAAAATATGGCTTCACAGATTTATAGATAATACCTGCATACCTCGAACCCGCCTTACCGCCATCGCCTGCGCCCGCTGAAGCACCTGAACCCTGGCTGCCTTGttt                                      <  1:246484/228‑1 (MQ=255)                                                                                                                                                                                                                      cgccCCACATAAATCATTTGGCCCGGAAACCTTTTGGTAAGATAAAATAGTGCCATCACTAGAAAGCTCAATTCTAATATCACAGACCTTACCAAAAAAACGACTATCACCACGAAAATATGGCTTCACAGATTTATAGATAATACCTGCATACCTCGAACCCGCCTTACCGCCATCGCCTGCGCCCGCTGAAGCACCTGAACCCTGGCTGCCTTGTTTATTTATGTTACTACCTTGCAAAGCACTgccg       >  1:447817/1‑250 (MQ=255)                                                                                                                                                                                                                          ccACATAAATCATTTGGCCCGGAAACCTTTTGGTAAGATAAAATAGTGCCATCACTAGAAAGCTCAATTCTAATATCACAGACCTTACCAAAAAAACGACTATCACCACGAAAATATGGCTTCACAGATTTATAGATAATACCTGCATACCTCGAACCCGCCTTACCGCCATCGCCTGCGCCCGCTGAAGCACCTGAACCCTGGCTGCCTTGTTTATTTATGTTACTACCTTGCAAAGCACTgccgccgcc  <  1:32133/251‑1 (MQ=255)                                                                                                                                                                                                                                    cATTTGGCCCGGAAACCTTTTGGTAAGATAAAATAGTGCCATCACTAGAAAGCTCAATTCTAata                                                                                                                                                                                  >  1:755565/1‑65 (MQ=255)                                                                                                                                                                                                                                    cATTTGGCCCGGAAACCTTTTGGTAAGATAAAATAGTGCCATCACTAGAAAGCTCAATTCTAATATCACAGACCTTACCAAAAAAACGACTATCACCACGAAAatat                                                                                                                                        >  1:356715/1‑107 (MQ=255)                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                    GCTCGGGCTACCATGGCAAACCCTAACAGCATAATAAATAAGCCAAAAATTCGAATAACTACTTTCATTTTTTCTCCTATTGTTTAAAAGTGCGGTGAAAATCTACCGCACTTTTCTTGGGTCAAAGGTTACAATGGACCTTTTAAATATTTCATATTCTTCCGGCGTAGGCGGTTCGTTCATTTTTCTGGTTTGACCAATAGCATTTAAAGCCGCCCCACATAAATCATTTGGCCCGGAGACCTTTTGGTAAGATAAAATAGTGCCATCACTAGAAAGCTCAATTCTAATATCACAGACCTTACCAAAAAAACGACTATCACCACGAAAATATGGCTTCACAGATTTATAGATAATACCTGCATACCTCGAACCCGCCTTACCGCCATCGCCTGCGCCCGCTGAAGCACCTGAACCCTGGCTGCCTTGTTTATTTATGTTACTACCTTGCAAAGCACTGCCGCCGCC  >  1/1995510‑1995977

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑