Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,994,062	A→G	V74A (GTA→GCA)	unknown ←	Tol‑Pal system peptidoglycan‑associated lipoprotein PAL

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,994,062	0	A	G	100.0%	221.8 / NA	72	V74A (GTA→GCA)	unknown	Tol‑Pal system peptidoglycan‑associated lipoprotein PAL
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (39/33);  total (39/33)

TGCTAATACTGCACGACGGTTTTTAGCATAAGCAGATTCGTCATGACCTAATACTGCCGGTTTTTCTTCACCGTAAGAAACGGTGGATATTTGACCTGCTTTTACACCTTTAGCAGTTAAAAAGTTTTGTACTGCACGTGCACGGCGTTCACCTAATGCCATATTATATTCCGGTGTACCACGCTCGTCGGTGTTACCTTCAACACGCACGCTTGTTGCAGGTGTTGCATTTAAGTATGCTACATGAGCATCTAAGATTTGAGTGTATTCACCTTCAATATCATATTTATCAAAGCCGAAATACACGGTGTTGTAGCGTTGTTGTAAATCTTGAACGGAATAGCCACCAAATGTTTGACCATTATTGCTACCACTACCGCTTTCGTTTTTGTTGGATGATCCACACGCCGCTAACACAGCCACAGAACCAGCAACTAACAATGCTTTCGTAAATTTATTCATTGATTTCTCCTTGTTTATTT  >  1/1993821‑1994302                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                 tGCTAATACTGCACGACGGTTTTTAGCATAAGCAGGTTCGTCATGACCTAATACTGCCGGTTTTTCTTCACCGTAAGAAACGGTGGATATTTGACCTGCTTTTACACCTTTAGCAGTTAAAAAGTTTTGTACTGCACGTGCACGGCGTTCACCTAATGCCATATTATATTCCGGTGTACCACGCTCGTCGGTGTTACCTTCAACACGCACGCTTGTTGCAGGTGTTGCATTTAAGTATGCTGCATGAGCAt                                                                                                                                                                                                                                         >  1:259138/1‑251 (MQ=255) tGCTAATACTGCACGACGGTTTTTAGCATAAGCAGATTCGTCATGACCTAATACTGCCGGTTTTTCTTCACCGTAAGAAACGGTGGATATTTGACCTGCTTTTACACCTTTAGCAGTTAAAAAGTTTTGTACTGCACGTGCACGGCGTTCACCTAATGCCATATTATATTCCGGTGTACCACGCTCGTCGGTGTTACCTTCAACACGCACGCTTGTTGCAGGTGTTGCATTTAAGTATGCTGCATGAGCAt                                                                                                                                                                                                                                         >  1:639743/1‑251 (MQ=255) tGCTAATACTGCACGACGGTTTTTAGCATAAGCAGATTCGTCATGACCTAATACTGCCGGTTTTTCTTCACCGTAAGAAACGGTGGATATTTGACCTGCTTTTACACC‑TTAGCAGTTAAAAAGTTTTGTACTGCACGTGCACGGCGTTCACCTAATGCCATATTATATTCCGGTGTACCACGCTCGTCGGTGTTACCTTCAACACGCACGCTTGTTGCAGGTGTTGCATTTAAGTATGCTGCATGAGCAt                                                                                                                                                                                                                                         >  1:676142/1‑250 (MQ=255)         cTGCACGACGGTTTTTAGCATAAGCAGATTCGTCATGACCTAATACTGCCGGTTTTTCTTCACCGTAAGAAACGGTGGATATTTGACCTGCTTTTACACCTTTAGCAGTTAAAAAGTTTTGTACTGCACGTGCACGGCGTTCACCTAATGCCATATTATATTCCGGTGTACCACGCTCGTCGGTGTTACCTTCAACACGCACGCTTGTTGCAGGTGTTGCATTTAAGTATGCTGCATGAGCATCTAAGAtt                                                                                                                                                                                                                                 <  1:68820/251‑1 (MQ=255)         cTGCACGACGGTTTTTAGCATAAGCAGATTCGTCATGACCTAATACTGCCGGTTTTTCTTCACCGTAAGAAACGGTGGATATTTGACCTGCTTTTACACCTTTAGCAGTTAAAAAGTTTTGTACTGCACGTGCACGGCGTTCACCTAATGCCATATTATATTCCGGTGTACCACGCTCGTCGGTGTTACCTTCAACACGCACGCTTGTTGCAGGTGTTGCATTTAAGTATGCTGCATGAGCATCTAAGAtt                                                                                                                                                                                                                                 <  1:695890/251‑1 (MQ=255)             acgacgGTTTTTAGCATAAGCAGATTCGTCATGACCTAATACTGCCGGTTTTTCTTCACCGTAAGAAACGGTGGATATTTGACCTGCTTTTACACCTTTAGCAGTTAAAAAGTTTTGTACTGCACGTGCACGGCGTTCACCTAATGCCATATTATATTCCGGTGTACCACGCTCGTCGGTGTTACCTTCAACACGCACGCTTGTTGCAGGTGTTGCATTTAAGTATGCTGCATGAGCATCTAAGATTTGAg                                                                                                                                                                                                                             <  1:232363/251‑1 (MQ=255)              cgacgGTTTTTAGCATAAGCAGATTCGTCATGACCTAATACTGCCGGTTTTTCTTCACCGTAAGAAACGGTGGATATTTGACCTGCTTTTACACCTTTAGCAGTTAAAAAGTTTTGTACTGCACGTGCACGGCGTTCACCTAATGCCATATTATATTCCGGTGTACCACGCTCGTCGGTGTTACCTTCAACACGCACGCTTGTTGCAGGTGTTGCATTTAAGTATGCTGCATGAGCATCTAAGATTTGAgt                                                                                                                                                                                                                            <  1:846469/251‑1 (MQ=255)                   gTTTTTAGCATAAGCAGATTCGTCATGACCTAATACTGCCGGTTTTTCTTCACCGTAAGAAACGGTGGATATTTGACCTGCTTTTACACCTTTAGCAGTTAAAAAGTTTTGTACTGCACGTGCACGGCGTTCACCTAATGCCATATTATATTCCGGTGTACCACGCTCGTCGGTGTTACCTTCAACACGCACGCTTGTTGCAGGTGTTGCATTTAAGTATGCTGCATGAGCATCTAAGATTTGAGTGTAtt                                                                                                                                                                                                                       >  1:622521/1‑251 (MQ=255)                   gTTTTTAGCATAAGCAGATTCGTCATGACCTAATACTGCCGGTTTTTCTTCACCGTAAGAAACGGTGGATATTTGACCTGCTTTTACACCTTTAGCAGTTAAAAAGTTTTGTACTGCACGTGCACGGCGTTCACCTAATGCCATATTATATTCCGGTGTACCACGCTCGTCGGTGTTACCTTCAACACGCACGCTTGTTGCAGGTGTTGCATTTAAGTATGCTGCATGAGCATCTAAGATTTGAGTGTAt                                                                                                                                                                                                                        <  1:158958/250‑1 (MQ=255)                                 cAGATTCGTCATGACCTAATACTGCCGGTTTTTCTTCACCGTAAGAAACGGTGGATATTTGACCTGCTTTTACACCTTTAGCAGTTAAAAAGTTTTGTACTGCACGTGCACGGCGTTCACCTAATGCCATATTATATTCCGGTGTACCACGCTCGTCGGTGTTACCTTCAACACGCACGCTTGTTGCAGGTGTTGCATTTAAGTATGCTGCATGAGCATCTAAGATTTGAGTGTATTCACCTTCAatat                                                                                                                                                                                                           <  1:5793/249‑1 (MQ=255)                                              aCCTAATACTGCCGGTTTTTCTTCACCGTAAGAAACGGTGGATATTTGACCTGCTTTTACACCTTTAGCAGTTAAAAAGTTTTGTACTGCACGTGCACGGCGTTCACCTAATGCCATATTATATTCCGGTGTACCACGCTCGTCGGTGTTACCTTCAACACGCACGCTTGTTGCAGGTGTTGCATTTAAGTATGCTGCATGAg                                                                                                                                                                                                                                            >  1:896587/1‑203 (MQ=255)                                              aCCTAATACTGCCGGTTTTTCTTCACCGTAAGAAACGGTGGATATTTGACCTGCTTTTACACCTTTAGCAGTTAAAAAGTTTTGTACTGCACGTGCACGGCGTTCACCTAATGCCATATTATATTCCGGTGTACCACGCTCGTCGGTGTTACCTTCAACACGCACGCTTGTTGCAGGTGTTGCATTTAAGTATGCTGCATGAGCATCTAAGa                                                                                                                                                                                                                                   <  1:112737/212‑1 (MQ=255)                                              aCCTAATACTGCCGGTTTTTCTTCACCGTAAGAAACGGTGGATATTTGACCTGCTTTTACACCTTTAGCAGTTAAAAAGTTTTGTACTGCACGTGCACGGCGTTCACCTAATGCCATATTATATTCCGGTGTACCACGCTCGTCGGTGTTACCTTCAACACGCACGCTTGTTGCAGGTGTTGCATTTAAGTATGCTGCATGAGCATCTAAGAt                                                                                                                                                                                                                                  <  1:404706/213‑1 (MQ=255)                                              aCCTAATACTGCCGGTTTTTCTTCACCGTAAGAAACGGTGGATATTTGACCTGCTTTTACACCTTTAGCAGTTAAAAAGTTTTGTACTGCACGTGCACGGCGTTCACCTAATGCCATATTATATTCCGGTGTACCACGCTCGTCGGTGTTACCTTCAACACGCACGCTTGTTGCAGGTGTTGCATTTAAGTATGCTGCATGAGCATCTAAGATTTGAGTGTATTCACCTTCAATATCATAttt                                                                                                                                                                                                    <  1:891676/243‑1 (MQ=255)                                              aCCTAATACTGCCGGTTTTTCTTCACCGTAAGAAACGGTGGATATTTGACCTGCTTTTACACCTTTAGCAGTTAAAAAGTTTTGTACTGCACGTGCACGGCGTTCACCTAATGCCATATTATATTCCGGTGTACCACGCTCGTCGGTGTTACCTTCAACACGCACGCTTGTTGCAGGTGTTGCATTTAAGTATGCTGCATGAGCATCTAAGATTTGAGTGTATTCACCTTCAATATCATATTTATCAAAg                                                                                                                                                                                             >  1:763530/1‑250 (MQ=255)                                               ccTAATACTGCCGGTTTTTCTTCACCGTAAGAAACGGTGGATATTTGACCTGCTTTTACACCTTTAGCAGTTAAAAAGTTTTGTACTGCACGTGCACGGCGTTCACCTAATGCCATATTATATTCCGGTGTACCACGCTCGTCGGTGTTACCTTCAACACGCACGCTTGTTGCAGGTGTTGCATTTAAGTATGCTGCATGAGCATCTAAGATTTGAGTGTATTCACCTTCAATATCATATTTATCAAAGcc                                                                                                                                                                                           <  1:399997/251‑1 (MQ=255)                                                      cTGCCGGTTTTTCTTCACCGTAAGAAACGGTGGATATTTGACCTGCTTTTACACCTTTAGCAGTTAAAAAGTTTTGTACTGCACGTGCACGGCGTTCACCTAATGCCATATTATATTCCGGTGTACCACGCTCGTCGGTGTTACCTTCAACACGCACGCTTGTTGCAGGTGTTGCATTTAAGTATGCTGCATGAGCATCTAAGATTTGAGTGTATTCACCTTCAATATCATATTTATCAAAGCCGAAATac                                                                                                                                                                                    <  1:413492/251‑1 (MQ=255)                                                             tttttCTTCACCGTAAGAAACGGTGGATATTTGACCTGCTTTTACACCTTTAGCAGTTAAAAAGTTTTGTACTGCACGTGCACGGCGTTCACCTAATGCCATATTATATTCCGGTGTACCACGCTCGTCGGTGTTACCTTCAACACGCACGCTTGTTGCAGGTGTTGCATTTAAGTATGCTGCATGAGCATCTAAGATTTGAGTGTATTCACCTTCAATATCATATTTATCAAAGCCGAAATACACGgtgt                                                                                                                                                                             <  1:855921/251‑1 (MQ=255)                                                                  cttcACCGTAAGAAACGGTGGATATTTGACCTGCTTTTACACCTTTAGCAGTTAAAAAGTTTTGTACTGCACGTGCACGGCGTTCACCTAATGCCATATTATATTCCGGTGTACCACGCTCGTCGGTGTTACCTTCAACACGCACGCTTGTTGCAGGTGTTGCATTTAAGTATGCTGCATGAGCATCTAAGATTTGAGTGTATTCACCTTCAATATCATATTTATCAAAGCCGAAATACACGGTGTTGTAg                                                                                                                                                                        <  1:132159/251‑1 (MQ=255)                                                                  cttcACCGTAAGAAACGGTGGATATTTGACCTGCTTTTACACCTTTAGCAGTTAAAAAGTTTTGTACTGCACGTGCACGGCGTTCACCTAATGCCATATTATATTCCGGTGTACCACGCTCGTCGGTGTTACCTTCAACACGCACGCTTGTTGCAGGTGTTGCATTTAAGTATGCTGCATGAGCATCTAAGATTTGAGTGTATTCACCTTCAATATCATATTTATCAAAGCCGAAATACACGGTGTTGTAg                                                                                                                                                                        <  1:839132/251‑1 (MQ=255)                                                                         gTAAGAAACGGTGGATATTTGACCTGCTTTTACACCTTTAGCAGTTAAAAAGTTTTGTACTGCACGTGCACGGCGTTCACCTAATGCCATATTATATTCCGGTGTACCACGCTCGTCGGTGTTACCTTCAACACGCACGCTTGTTGCAGGTGTTGCATTTAAGTATGCTGCATGAGCATCTAAGATTTGAGTGTATTCACCTTCAATATCATATTTATCAAAGCCGAAATACACGGTGTTGTAGCg                                                                                                                                                                      >  1:485404/1‑246 (MQ=255)                                                                          tAAGAAACGGTGGATATTTGACCTGCTTTTACACCTTTAGCAGTTAAAAAGTTTTGTACTGCACGTGCACGGCGTTCACCTAATGCCATATTATATTCCGGTGTACCACGCTCGTCGGTGTTACCTTCAACACGCACGCTTGTTGCAGGTGTTGCATTTAAGTATGCTGCATGAGCATCTAAGATTTGAGTGTATTCACCTTCAATATCATATTTATCAAAGCCGAAATACACGGTGTTGTAGCGttgttg                                                                                                                                                                <  1:208034/251‑1 (MQ=255)                                                                                  ggTGGATATTTGACCTGCTTTTACACCTTTAGCAGTTAAAAAGTTTTGTACTGCACGTGCACGGCGTTCACCTAATGCCATATTATATTCCGGTGTACCACGCTCGTCGGTGTTACCTTCAACACGCACGCTTGTTGCAGGTGTTGCATTTAAGTATGCTGCATGAGCATCTAAGATTTGAGTGTATTCACCTTCAATATCATATTTATCAAAGCCGAAATACACGGTGTTGTAGCGTTGTTGTAAATCtt                                                                                                                                                        >  1:897226/1‑251 (MQ=255)                                                                                   gTGGATATTTGACCTGCTTTTACACCTTTAGCAGTTAAAAAGTTTTGTACTGCACGTGCACGGCGTTCACCTAATGCCATATTATATTCCGGTGTACCACGCTCGTCGGTGTTACCTTCAACACGCACGCTTGTTGCAGGTGTTGCATTTAAGTATGCTGCATGAGCATCTAAGATTTGAGTGTATTCACCTTCAATATCatat                                                                                                                                                                                                      >  1:639858/1‑204 (MQ=255)                                                                                     ggATATTTGACCTGCTTTTACACCTTTAGCAGTTAAAAAGTTTTGTACTGCACGTGCACGGCGTTCACCTAATGCCATATTATATTCCGGTGTACCACGCTCGTCGGTGTTACCTTCAACACGCACGCTTGTTGCAGGTGTTGCATTTAAGTATGCTGCATGAGCATCTAAGATTTGAGTGTATTCACCTTCAATATCATATTTATCAAAGCCGAAATACACGGTGTTGTAg                                                                                                                                                                        >  1:762685/1‑232 (MQ=255)                                                                                                  gCTTTTACACCTTTAGCAGTTAAAAAGTTTTGTACTGCACGTGCACGGCGTTCACCTAATGCCATATTATATTCCGGTGTACCACGCTCGTCGGTGTTACCTTCAACACGCACGCTTGTTGCAGGTGTTGCATTTAAGTATGCTGCATGAGCATCTAAGATTTGAGTGTATTCACCTTCAATATCATATTTATCAAAGCCGAAATACACGGTGTTGTAGCGTTGTTGTAAATCTTGAACGGAATAGccac                                                                                                                                         >  1:311479/1‑250 (MQ=255)                                                                                                           ccTTTAGCAGTTAAAAAGTTTTGTACTGCACGTGCACGGCGTTCACCTAATGCCATATTATATTCCGGTGTACCACGCTCGTCGGTGTTACCTTCAACACGCACGCTTGTTGCAGGTGTTGCATTTAAGTATGCTGCATGAGCATCTAAGATTTGAGTGTATTCACCTTCAATATCATATTTATCAAAGCCGAAATACACGGTGTTGTAGCGTTGTTGTa                                                                                                                                                              >  1:205230/1‑220 (MQ=255)                                                                                                           ccTTTAGCAGTTAAAAAGTTTTGTACTGCACGTGCACGGCGTTCACCTAATGCCATATTATATTCCGGTGTACCACGCTCGTCGGTGTTACCTTCAACACGCACGCTTGTTGCAGGTGTTGCATTTAAGTATGCTGCATGAGCATCTAAGATTTGAGTGTATTCACCTTCAATATCATATTTATCAAAGCCGAAATACACGGTGTTGTAGCGTTGTTGTAAATCTTGAACGGAATAGccac                                                                                                                                         <  1:560942/241‑1 (MQ=255)                                                                                                                    gTTAAAAAGTTTTGTACTGCACGTGCACGGCGTTCACCTAATGCCATATTATATTCCGGTGTACCACGCTCGTCGGTGTTACCTTCAACACGCACGCTTGTTGCAGGTGTTGCATTTAAGTATGCTGCATGAg                                                                                                                                                                                                                                            <  1:689548/133‑1 (MQ=255)                                                                                                                     ttAAAAAGTTTTGTACTGCACGTGCACGGCGTTCACCTAATGCCATATTATATTCCGGTGTACCACGCTCGTCGGTGTTACCTTCAACACGCACGCTTGTTGCAGGTGTTGCATTTAAGTATGCTGCATGAGCATCTAAGATTTGAGTGTATTCACCTTCAATATCATATTTATCAAAGCCGAAATACACGGTGTTGTAGCGTTGTTGTAAATCTTGAACGGAATAGCCACCAAATGTTTGACCattat                                                                                                                       <  1:110803/249‑1 (MQ=255)                                                                                                                         aaaGTTTTGTACTGCACGTGCACGGCGTTCACCTAATGCCATATTATATTCCGGTGTACCACGCTCGTCGGTGTTACCTTCAACACGCACGCTTGTTGCAGGTGTTGCATTTAAGTATGCTGCATGAGCATCTAAGATTTGAGTGTATTCACCTTCAATATCATATTTATCAAAGCCGAAATACACGGTGTTGTAGCGTTGTTGTAAATCTTGAACGGAATAGCCACCAAATGTTTGACCATTATTGCTAc                                                                                                                 <  1:604570/251‑1 (MQ=255)                                                                                                                            gTTTTGTACTGCACGTGCACGGCGTTCACCTAATGCCATATTATATACCGGTGTACCACGCTCGTCGGTGTTACCTTCAACACGCACGCTTGTTGCAGGTGTTGCATTTAAGTATGCTGCATGAGCATCTAAGATTTGAGTGTATTCACCTTCAATATCATATTTATCACAGCCGAAATACACGGTGTTGTAGCGTTGTTGTAAATCTTGAACGGAATAGCCACCAAATGTTTGACCATTATTGCTACCa                                                                                                               >  1:435272/1‑250 (MQ=255)                                                                                                                                      gcacgtgcacgGCGTTCACCTAATGCCATATTATATTCCGGTGTACCACGCTCGTCGGTGTTACCTTCAACACGCACGCTTGTTGCAGGTGTTGCATTTAAGTATGCTGCATGAGCATCTAAGATTTGAGTGTATTCACCTTCAatat                                                                                                                                                                                                           >  1:696296/1‑148 (MQ=255)                                                                                                                                      gcacgtgcacgGCGTTCACCTAATGCCATATTATATTCCGGTGTACCACGCTCGTCGGTGTTACCTTCAACACGCACGCTTGTTGCAGGTGTTGCATTTAAGTATGCTGCATGAGCATCTAAGATTTGAGTGTATTCACCTTCAATATCATATTTATc                                                                                                                                                                                                 >  1:338936/1‑158 (MQ=255)                                                                                                                                      gcacgtgcacgGCGTTCACCTAATGCCATATTATATTCCGGTGTACCACGCTCGTCGGTGTTACCTTCAACACGCACGCTTGTTGCAGGTGTTGCATTTAAGTATGCTGCATGAGCATCTAAGATTTGAGTGTATTCACCTTCAATATCATATTTATCAAAGCCGAAATACACGGTGTTGTAGCGTTGTTGTAAATCTTGAACGGAATAGCCACCAAATGTTTGACCATTATTGCTACCACTACCGCttt                                                                                                     >  1:61832/1‑250 (MQ=255)                                                                                                                                         cgtgcacgGCGTTCACCTAATGCCATATTATATTCCGGTGTACCACGCTCGTCGGTGTTACCTTCAACACGCACGCTTGTTGCAGGTGTTGCATTTAAGTATGCTGCATGAGCATCTAAGATTTGAGTGTATTCACCTTCAATATCatat                                                                                                                                                                                                      >  1:206761/1‑150 (MQ=255)                                                                                                                                                 gCGTTCACCTAATGCCATATTATATTCCGGTGTACCACGCTCGTCGGTGTTACCTTCAACACGCACGCTTGTTGCAGGTGTTGCATTTAAGTATGCTGCATGAGCATCTAAGATTTGAGTGTATTCACCTTCAATATCATATTTATCAAAGCCGAAATACACGGTGTTGTAGCGTTGTTGTAAATCTTGAACGGAATAGCCACCAAATGTTTGACCATTATTGCTACCACTACCGCTTTCGTTTTTGTTgg                                                                                         >  1:783290/1‑251 (MQ=255)                                                                                                                                                       aCCTAATGCCATATTATATTCCGGTGTACCACGCTCGTCGGTGTTACCTTCAACACGCACGCTTGTTGCAGGTGTTGCATTTAAGTATGCTGCATGAGCATCTAAGATTTGAGTGTATTCACCTTCAATATCATATTTATCAAAGCCGAAATACACGGTGTTGTAGCGTTGTTGTAAATCTTGAACGGAATAGCCACCAAATGTTTGACCATTATTGCTACCACTACCGc                                                                                                        <  1:188488/230‑1 (MQ=255)                                                                                                                                                        ccTAATGCCATATTATATTCCGGTGTACCACGCTCGTCGGTGTTACCTTCAACACGCACGCTTGTTGCAGGTGTTGCATTTAAGTATGCTGCATGAGCa                                                                                                                                                                                                                                          >  1:208803/1‑99 (MQ=255)                                                                                                                                                           aaTGCCATATTATATTCCGGTGTACCACGCTCGTCGGTGTTACCTTCAACACGCACGCTTGTTGCAGGTGTTGCATTTAAGTATGCTGCATGAGCATCTAAGATTTGAGTGTATTCACCTTCAATATCATATTTATCAAAGCCGAAATACACGGTGTTGTAGCGTTGTTGTAAATCTTGAACGGAATAGCCACCAAATGTTTGACCATTATTGCTACCACTACCGCTTTCGTTTTTGTTGGATGATCcaca                                                                               <  1:372531/251‑1 (MQ=255)                                                                                                                                                            aTGCCATATTATATTCCGGTGTACCACGCTCGTCGGTGTTACCTTCAACACGCACGCTTGTTGCAGGTGTTGCATTTAAGTATGCTGCATGAGCATCTAAGATTTGAGTGTATTCACCTTCAATATCATATTTATCAAAGCCGAAATACACGGTGTTGTAg                                                                                                                                                                        <  1:560084/161‑1 (MQ=255)                                                                                                                                                              gCCATATTATATTCCGGTGTACCACGCTCGTCGGTGTTACCTTCAACACGCACGCTTGTTGCAGGTGTTGCATTTAAGTATGCTGCATGAGCATCTAAGATTTGAGTGTATTCACCTTCAATATCATATTTATCAAAGCCGAAATACACGGTGTTGTAGCGTTGTTGTAAATCTTGAACGGAATAGCCACCAAATGTTTGACCATTATTGCTACCACTACCGCTTTCGTTTTTGTTGGATGATCCACAcg                                                                             >  1:25436/1‑250 (MQ=255)                                                                                                                                                                 atattatattCCGGTGTACCACGCTCGTCGGTGTTACCTTCAACACGCACGCTTGTTGCAGGTGTTGCATTTAAGTATGCTGCATGAGCATCTAAGATTTGAGTGTATTCACCTTCAATATCATATTTATCAAAGCCGAAATACACGGTGTTGTAGCGTTGTTGTAAATCTTGAACGGAATAGCCACCAAATGTTTGACCATTATTGCTACCACTACCg                                                                                                         <  1:657009/219‑1 (MQ=255)                                                                                                                                                                   attatattCCGGTGTACCACGCTCGTCGGTGTTACCTTCAACACGCACGCTTGTTGCAGGTGTTGCATTTAAGTATGCTGCATGAGCATCTAAGATTTGAGTGTATTCACCTTCAATATCATATTTATCAAAGCCGAAATACACGGTGTTGTAGCGTTGTTGTAAATCTTGAACGGAATAGCCACCAAATGTTTGACCATTATTGCTACCACTACCGCTTTCGTTTTTGTTGGATGATCCacac                                                                              <  1:848098/244‑1 (MQ=255)                                                                                                                                                                   attatattCCGGTGTACCACGCTCGTCGGTGTTACCTTCAACACGCACGCTTGTTGCAGGTGTTGCATTTAAGTATGCTGCATGAGCATCTAAGATTTGAGTGTATTCACCTTCAATATCATATTTATCAAAGCCGAAATACACGGTGTTGTAGCGTTGTTGTAAATCTTGAACGGAATAGCCACCAAATGTTTGACCATTATTGCTACCACTACCGCTTTCGTTTTTGTTGGATGATCCACACGCCGCTa                                                                       >  1:737656/1‑251 (MQ=255)                                                                                                                                                                   attatattCCGGTGTACCACGCTCGTCGGTGTTACCTTCAACACGCACGCTTGTTGCAGGTGTTGCATTTAAGTATGCTGCATGAGCATCTAAGATTTGAGTGTATTCACCTTCAATATCATATTTATCAAAGCCGAAATACACGGTGTTGTAGCGTTGTTGTAAATCTTGAACGGAATAGCCACCAAATGTTTGACCATTATTGCTACCACTACCGCTTTCGTTTTTGTTGGATGATCCACACGCCGCTa                                                                       >  1:172506/1‑251 (MQ=255)                                                                                                                                                                   attatattCCGGTGTACCACGCTCGTCGGTGTTACCTTCAACACGCACGCTTGTTGCAGGTGTTGCATTTAAGTATGCTGCATGAGCATCTAAGATTTGAGTGTATTCACCTTCAATATCATATTTATCAAAGCCGAAATACACGGTGTTGTAGCGTTGTTGTAAATCTTGAACGGAATAGCCACCAAATGTTTGACCATTATTGCTACCACTACCGCTTTCGTTTTTGTTGGATGATCCACACGCCGCTa                                                                       >  1:596808/1‑251 (MQ=255)                                                                                                                                                                   attatattCCGGTGTACCACGCTCGTCGGTGTTACCTTCAACACGCACGCTTGTTGCAGGTGTTGCATTTAAGTATGCTGCATGAGCATCTAAGATTTGAGTGTATTCACCTTCAATATCATATTTATCAAAGCCGAAATACACGGTGTTGTAGCGTTGTTGTAAATCTTGAACGGAATAGCCACCAAATGTTTGACCATTATTGCTACCACTACCGCTTTCGTTTTTGTTGGATGATCCACACGCCGCTa                                                                       >  1:415895/1‑251 (MQ=255)                                                                                                                                                                       tattCCGGTGTACCACGCTCGTCGGTGTTACCTTCAACACGCACGCTTGTTGCAGGTGTTGCATTTAAGTATGCTGCATGAGCATCTAAGATTTGAGTGTATTCACCTTCAATATCATATTTATCAAAGCCGAAATACACGGTGTTGTAGCGTTGTTGTAAATCTTGAACGGAATAGCCACCAAATGTTTGACCATTATTGCTACCACTACCGCTTTCGTTTTTGTTGGATGATCCACACGCCGCTAacac                                                                   <  1:461463/251‑1 (MQ=255)                                                                                                                                                                          tCCGGTGTACCACGCTCGTCGGTGTTACCTTCAACACGCACGCTTGTTGCAGGTGTTGCATTTAAGTATGCTGCATGAGCATCTAAGATTTGAGTGTATTCACCTTCAATATCATATTTATCAAAGCCGAAATACACGGTGTTGTAGCGTTGTTGTAAATCTTGAACGGAATAGCCACCAAATGTTTGACCATTATTGCTACCACTACCGCTTTCGTTTTTGTTGGATGATCCACACGCCGCTAACACAGc                                                                <  1:337027/251‑1 (MQ=255)                                                                                                                                                                           ccGGTGTACCACGCTCGTCGGTGTTACCTTCAACACGCACGCTTGTTGCAGGTGTTGCATTTAAGTATGCTGCATGAGCATCTAAGATTTGAGTGTATTCACCTTCAATATCATATTTATCAAAGCCGAAATACACGGTGTTGTAGCGTTGTTGTAAATCTTGAACGGAATAGCCACCAAATGTTTGACCATTATTGCTACCACTACCGCTTTCGTTTTTGTTGGATGATCCACACGCCGCTAACACAGc                                                                >  1:177512/1‑250 (MQ=255)                                                                                                                                                                           ccGGTGTACCACGCTCGTCGGTGTTACCTTCAACACGCACGCTTGTTGCAGGTGTTGCATTTAAGTATGCTGCATGAGCATCTAAGATTTGAGTGTATTCACCTTCAATATCATATTTATCAAAGCCGAAATACACGGTGTTGTAGCGTTGTTGTAAATCTTGAACGGAATAGCCACCAAATGTTTGACCATTATTGCTACCACTACCGCTTTCGTTTTTGTTGGATGATCCACACGCCGCTAACACAGc                                                                >  1:517244/1‑250 (MQ=255)                                                                                                                                                                           ccGGTGTACCACGCTCGTCGGTGTTACCTTCAACACGCACGCTTGTTGCAGGTGTTGCATTTAAGTATGCTGCATGAGCATCTAAGATTTGAGTGTATTCACCTTCAATATCATATTTATCAAAGCCGAAATACACGGTGTTGTAGCGTTGTTGTAAAACTTGAACGGAATAGCCACCAAATGTTTGACCATTATTGCTACCACTACCGCTTTCGTTTTTGTTGGATGATCCACACGCCGCTAACACAGc                                                                >  1:493588/1‑250 (MQ=255)                                                                                                                                                                                gtACCACGCTCGTCGGTGTTACCTTCAACACGCACGCTTGTTGCAGGTGTTGCATTTAAGTATGCTGCATGAGCATCTAAGATTTGAGTGTATTCACCTTCAATATCATATTTATCAAAGCCGAAATACACGGTGTTGTAGCGTTGTTGTAAATCTTGAACGGAATAGCCACCAAATGTTTGACCATTATTGCTACCACTACCGCTTTCGTTTTTGTTGGATGATCCACACGCCGCTAACACAGCCACAGa                                                          >  1:603514/1‑251 (MQ=255)                                                                                                                                                                                 tACCACGCTCGTCGGTGTTACCTTCAACACGCACGCTTGTTGCAGGTGTTGCATTTAAGTATGCTGCATGAGCATCTAAGATTTGAGTGTATTCACCTTCAATATCATATTTATCAAAGCCGAAATACACGGTGTTGTAGCGTTGTTGTAAATCTTGAACGGAATAGCCACCAAATGTTTGACCATTATTGCTACCACTACCGCTTTCGTTTTTGTTGGATGATCCACACGCCGCTAACACAGCCACAGaa                                                         <  1:180722/251‑1 (MQ=255)                                                                                                                                                                                  cccACGCTCGTCGGTGTTACCTTCAACACGCACGCTTGTTGCAGGTGTTGCATTTAAGTATGCTGCATGAGCATCTAAGATTTGAGTGTATTCCCCTTCAATATCATATTTATCAAAGCCGAAATACACGGTGTTGTAGCGTTGTTGTAAATCTTGACCGGAATAGCCACCAAATGTTTGACCATTATTGCTACCACTACCGCTTTCGTTTTTGTTGGATGATCCACACGCCGCTAACACAGCCACAGAAc                                                        <  1:379695/250‑1 (MQ=255)                                                                                                                                                                                  aCCACGCTCGTCGGTGTTACCTTCAACACGCACGCTTGTTGCAGGTGTTGCATTTAAGTATGCTGCATGAGCATCTAAGATTTGAGTGTATTCACCTTCAATATCa                                                                                                                                                                                                         >  1:500569/1‑106 (MQ=255)                                                                                                                                                                                   ccACGCTCGTCGGTGTTACCTTCAACACGCACGCTTGTTGCAGGTGTTGCATTTAAGTATGCTGCATGAGCATCTAAGATTTGAGTGTATTCACCTTAAATATCATATTTATCAAAGCCGAAATACACGGTGTTGTAGCGTTGTTGTAAATCTTGAACGGAATAGCCACCAAATGTTTGACCATAATGGCTACCACTACCGCTTTCGTTTTTGTTGGATGTTCCACACGCCGCTAACACAGCCACAGAAcc                                                       <  1:860322/251‑1 (MQ=255)                                                                                                                                                                                        cTCGTCGGTGTTACCTTCAACACGCACGCTTGTTGCAGGTGTTGCATTTAAGTATGCTGCATGAGCATCTAAGATTTGAGTGTATTCAcc                                                                                                                                                                                                                   <  1:217636/90‑1 (MQ=255)                                                                                                                                                                                          cgtcgGTGTTACCTTCAACACGCACGCTTGTTGCAGGTGTTGCATTTAAGTATGCTGCATGAGCATCTAAGATTTGAGTGTATTCACCTTCAATATCatat                                                                                                                                                                                                      <  1:155718/101‑1 (MQ=255)                                                                                                                                                                                               gtgtTACCTTCAACACGCACGCTTGTTGCAGGTGTTGCATTTAAGTATGCTGCATGAGCATCTAAGATTTGAGTGTATTCACCTTCAATATCATATTTATCAAAGCCGAAATACACGGTGTTGTAGCGTTGTTGTAAATCTTGAACGGAATAGCCACCAAATGTTTGACCATTATTGCTACCACTACCGCTTTCGTTTTTGTTGGATGATCCACACGCCGCTAacac                                                                   >  1:188229/1‑227 (MQ=255)                                                                                                                                                                                                 gtTACCTTCAACACGCACGCTTGTTGCAGGTGTTGCATTTAAGTATGCTGCATGAGCATCTAAGATTTGAGTGTATTCACCTTCAATATCATATTTATCAAAGCCGAAATACACGGTGTTGTAGCGTTGTTGTAAATCTTGAACGGAATAGCCACCAAATGTTTGACCATTATTGCTACCACTACCGCTTTCGTTTTTGTTGGATGATCCACACGCCGCTAACACAGCCACAGAACCAGCAACTAACAATg                                         >  1:438622/1‑251 (MQ=255)                                                                                                                                                                                                      cTTCAACACGCACGCTTGTTGCAGGTGTTGCATTTAAGTATGCTGCATGAGCATCTAAGATTTGAGTGTATTCACCTTCAATATCATATTTATCAAAGCCGAAATACACGGTGTTGTAGCGTTGTTGTAAATCTTGAACGGAATAGCCACCAAATGTTTGACCATTATTGCTACCACTACCGCTTTCGTTTTTGTTGGATGATCCACAcgc                                                                            <  1:92199/211‑1 (MQ=255)                                                                                                                                                                                                                  cgcTTGTTGCAGGTGTTGCATTTAAGTATGCTGCATGAGCATCTAAGATTTGAGTGTATTCACCTTCAata                                                                                                                                                                                                            >  1:4089/1‑71 (MQ=255)                                                                                                                                                                                                                  cgcTTGTTGCAGGTGTTGCATTTAAGTATGCTGCATGAGCATCTAAGATTTGAGTGTATTCACCTTCAATATCATATTTATCAAAGCCGAAATACACGGTGTTGTAGCGTTGTTGTAAATCTTGAACGGAATAGCCACCAAATGTTTGACCATTATTGCTACCACTACCGCTTTCGTTTTTGTTGGATGATCCACACGCCGCTAACACAGCCACAGaa                                                         >  1:351768/1‑218 (MQ=255)                                                                                                                                                                                                                    cTTGTTGCAGGTGTTGCATTTAAGTATGCTGCATGAGCATCTAAGATTTGAGTGTATTCACCTTCAATATCATATTTATCAAAGCCGAAATACACGGTGTTGTAGCGTTGTTGTAAATCTTGAACGGAATAGCCACCAAATGTTTGACCATTATTGCTACCACTACCGCTTTCGTTTTTGTTGGATGATCCACACGCCGCTAACACAGCCACAGAACCAGCAACTAACAATGCTTTCGTAAATTTATTCAt                      >  1:187026/1‑251 (MQ=255)                                                                                                                                                                                                                      tgttgCAGGTGTTGCATTTAAGTATGCTGCATGAGCATCTAAGATTTGAGTGTATTCACCTTCAATATCATATTTATCAAAGCCGAAATACACGGTGTTGTAGCGTTGTTGTAAATCTTGAACGGAATAGCCACCaaa                                                                                                                                     <  1:719661/138‑1 (MQ=255)                                                                                                                                                                                                                                   gCATTTAAGTATGCTGCATGAGCATCTAAGATTTGAGTGTATTCACCTTCAATATCATATTTATCAAAGCCGAAATACACGGTGTTGTAGCGTTGTTGTAAATCTTGAACGGAATAGCCACCAAATGTTTGACCATTATTGCTACCACTACCGCTTTCGTTTTTGTTGGATGATCCACACGCCGCTAACACAGCCACAGAACCAGCAACTAACAATGCTTTCGTAAATTTATTCATTGATTTCTCCt           >  1:875333/1‑247 (MQ=255)                                                                                                                                                                                                                                   gCATTTAAGTATGCTGCATGAGCATCTAAGATTTGAGTGTATTCACCTTCAATATCATATTTATCAAAGCCGAAATACACGGTGTTGTAGCGTTGTTGTAAATCTTGAACGGAATAGCCACCAAATGTTTGACCATTATTGCTACCACTACCGCTTTCGTTTTTGTTGGATGATCCACACGCCGCTAACACAGCCACAGAACCAGCAACTAACAATGCTTTCGTAAATTTATTCATTGATTTCTCCTTGtt       >  1:75278/1‑251 (MQ=255)                                                                                                                                                                                                                                      tttAAGTATGCTGCATGAGCATCTAAGATTTGAGTGTATTCACCTTCAATATCATATTTATCAAAGCCGAAATACACGGTGTTGTAGCGTTGTTGTAAATCTTGAACGGAATAGCCACCAAATGTTTGACCATTATTGCTACCACTACCGCTTTCGTTTTTGTTGGATGATCCACACGCCGCTAACACAGCCACAGAACCAGCAACTAACAATGCTTTCGTAAATTTATTCATTGATTTCTCCTTGtttat    >  1:534063/1‑251 (MQ=255)                                                                                                                                                                                                                                        tAAGTATGCTGCATGAGCATCTAAGATTTGAGTGTATTCACCTTCAATATCATATTTATCAAAGCCGAAATACACGGTGTTGTAGCGTTGTTGTAAATCTTGAACGGAATAGCCACCAAATGTTTGACCATTATTGCTACCACTACCGCTTTCGTTTTTGTTGGATGATCCACACGCCGCTAACACAGCCACAGAACCAGCAACTAAc                                             >  1:176658/1‑208 (MQ=255)                                                                                                                                                                                                                                        tAAGTATGCTGCATGAGCATCTAAGATTTGAGTGTATTCACCTTCAATATCATATTTATCAAAGCCGAAATACACGGTGTTGTAGCGTTGTTGTAAATCTTGAACGGAATAGCCACCAAATGTTTGACCATTATTGCTACCACTACCGCTTTCGTTTTTGTTGGATGATCCACACGCCGCTAACACAGCCACAGAACCAGCAACTAACAATGCTTTCGTAAATTTATTCATTGATTTCTCCTTGtttattt  >  1:436508/1‑251 (MQ=255)                                                                                                                                                                                                                                              tgctgcATGAGCATCTAAGATTTGAGTGTATTCACCTTCAATATCATATTTATCAAAGCCGAAATACACGGTGTTGTAGCGTTGTTGTAAATCTTGAACGGAATAGCCACCAAATGTTTGACCATTATTGCTACCACTACCGCTTTCGTTTTTGTTGGATGATCCACACGCCGCTAACACAGCCACAGAACCAGCa                                                   <  1:879659/196‑1 (MQ=255)                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                 TGCTAATACTGCACGACGGTTTTTAGCATAAGCAGATTCGTCATGACCTAATACTGCCGGTTTTTCTTCACCGTAAGAAACGGTGGATATTTGACCTGCTTTTACACCTTTAGCAGTTAAAAAGTTTTGTACTGCACGTGCACGGCGTTCACCTAATGCCATATTATATTCCGGTGTACCACGCTCGTCGGTGTTACCTTCAACACGCACGCTTGTTGCAGGTGTTGCATTTAAGTATGCTACATGAGCATCTAAGATTTGAGTGTATTCACCTTCAATATCATATTTATCAAAGCCGAAATACACGGTGTTGTAGCGTTGTTGTAAATCTTGAACGGAATAGCCACCAAATGTTTGACCATTATTGCTACCACTACCGCTTTCGTTTTTGTTGGATGATCCACACGCCGCTAACACAGCCACAGAACCAGCAACTAACAATGCTTTCGTAAATTTATTCATTGATTTCTCCTTGTTTATTT  >  1/1993821‑1994302

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑