Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,990,041	T→C	L549L (TTA→TTG)	unknown ←	Exodeoxyribonuclease V gamma chain (EC 3.1.11.5)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,990,041	0	T	C	100.0%	200.9 / NA	68	L549L (TTA→TTG)	unknown	Exodeoxyribonuclease V gamma chain (EC 3.1.11.5)
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (35/33);  total (35/33)

CTTTAATATAAAACAGGGTTTCATCGGTTTCCGCCGAGGAGGCGAAGAATTTATCGGTGAGCGCCAGTAAATGCTGTTGCCATTGTTCCACGGAATAACGGTTTTGCAGCGTTTGGAACCAGTCGAATAACGCCGCTAAAAATGCCGCCAATTGCCCTGCCAGTTGCCCTTTCAAACCGAAACTGTTATCCAGCCCGAGGCTATCCTGCCAAATGCCGTTTTCTTCGCGCAAGGCATAACCCAATAACATTCGCTCCAAGCCGGCTTGCCATGAATTGTAGTTACTTTCCGCCTCATCATTTTTTGCTAAGCCGAAACGCACGCCGGCATTTTTCACCCAATCACGAATTTGCGCCAAATCCTGCAATTCAATATTAAAGCGTTGGCGAATTGCCGGCACATCAAGCAATGCCAGCACGTCTTCGGCACTAAACGTGCTTTCTTTCAGCTGCAATAAGGTCAGAAAACCGGCGATAATCACATTATTCTC  >  1/1989797‑1990286                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                      ctTTAATATAAAACAGGGTTTCATCGGTTTCCGCCGTGGCGGAGAAGAATTTATCGGTGAGCGCCAGTGAATGCTGTTGCCATTGTTCCATGGAATAACGGCTTTGCAGCGTTTGGAACCAATCAAATAACGCTGCTAAAAATGCCGCCAATTGCCCCGCCAGCTGCCCTTTTAAGCCGAAACTGTTATCCAGCCCGAGGCTGTCTTGCCAAATGCCGTTTTCTTCACGCAAGGCATAGCCCAACAACAtt                                                                                                                                                                                                                                                 >  1:89547/1‑251 (MQ=255) ctTTAATATAAAACAGGGTTTCATCGGTTTCCGCCGTGGCGGAGAAGAATTTATCGGTGAGCGCCAGTAAATGCTGTTGCCATTGTTCCATGGAATAACGGCTTTGCAGCGTTTGGAACCAATCAAATAACGCTGCTAAAAATGCCGCCAATTGCCCCGCCAGCTGCCCTTTTAAGCCGAAACTGTTATCCAGCCCGAGGCTGTCTTGCCAAATGCCGTTTTCTTCACGCAAGGCATAGCCCAACAACAtt                                                                                                                                                                                                                                                 >  1:876212/1‑251 (MQ=255) ctTTAATATAAAACAGGGTTTCATCGGTTTCCGCCGTGGCGGAGAAGAATTTATCGGTGAGCGCCAGTAAATGCTGTTGCCATTGTTCCATGGAATAACGGCTTTGCAGCGTTTGGAACCAATCAAATAACGCTGCTAAAAATGCCGCCAATTGCCCCGCCAGCTGCCCTTTTAAGCCGAAACTGTTATCCAGCCCGAGGCTGTCTTGCCAAATGCCGTTTTCTTCACGCAAGGCATAGCCCAACAACAtt                                                                                                                                                                                                                                                 >  1:440998/1‑251 (MQ=255) ctTTAATATAAAACAGGGTTTCATCGGTTTCCGCCGTGGCGGAGAAGAATTTATCGGTGAGCGCCAGTAAATGCTGTTGCCATGGTTCCATGGAATAACGGCTTTGCAGCGTTTGGAACCAATCAAATAACGCTGCTAAAAATGCCGCCAATTGCCCCGCCAGCTGCCCTTTTAAGCCGAAACTGTTATCCAGCCCGAGGCTGTCTTGCCAAATGCCGTTTTCTTCACGCAAGGCATAGCCCaacaac                                                                                                                                                                                                                                                    <  1:404094/248‑1 (MQ=255)      atatAAAACAGGGTTTCATCGGTTTCCGCCGTGGCGGAGAAGAATTTATCGGTGAGCGCCAGTAAATGCTGTTGCCATTGTTCCATGGAATAACGGCTTTGCAGCGTTTGGAACCAATCAAATAACGCTGCTAAAAATGCCGCCAATTGCCCCGCCAGCTGCCCTTTTAAGCCGAAACTGTTATCCAGCCCGAGGCTGTCTTGCCAAATGCCGTTTTCTTCACGCAAGGCATAGCCCaacaac                                                                                                                                                                                                                                                    <  1:451778/243‑1 (MQ=255)      atatAAAACAGGGTTTCATCGGTTTCCGCCGTGGCGGAGAAGAATTTATCGGTGAGCGCCAGTAAATGCTGTTGCCATTGTTCCATGGAATAACGGCTTTGCAGCGTTTGGAACCAATCAAATAACGCTGCTAAAAATGCCGCCAATTGCCCCGCCAGCTGCCCTTTTAAGCCGAAACTGTTATCCAGCCCGAGGCTGTCTTGCCAAATGCCGTTTTCTTCACGCAAGGCATAGCCCAACAACATTCGCt                                                                                                                                                                                                                                             >  1:368621/1‑250 (MQ=255)      atatAAAACAGGGTTTCATCGGTTTCCGCCGTGGCGGAGAAGAATTTATCGGTGAGCGCCAGTAAATGCTGTTGCCATTGTTCCATGGAATAACGGCTTTGCAGCGTTTGGAACCAATCAAATAACGCTGCTAAAAATGCCGCCAATTGCCCCGCCAGCTGCCCTTTTAAGCCGAAACTGTTATCCAGCCCGAGGCTGTCTTGCCAAATGCCGTTTTCTTCACGCAAGGCATAGCCCAACAACATTCGCt                                                                                                                                                                                                                                             >  1:496790/1‑250 (MQ=255)            aaCAGGGTTTCATCGGTTTCCGCCGTGGCGGAGAAGAATTTATCGGTGAGCGCCAGTAAATGCTGTTGCCATTGTTCCATGGAATAACGGCTTTGCAGCGTTTGGAACCAATCAAATAACGCTGCTAAAAATGCCGCCAATTGCCCCGCCAGCTGCCCTTTTAAGCCGAAACTGTTATCCAGCCCGAGGCTGTCTTGCCAAATGCCGTTTTCTTCACGCAAGGCATAGCCCAACAACATTCGCTCCAAGcc                                                                                                                                                                                                                                      <  1:593376/251‑1 (MQ=255)            aaCAGGGTTTCATCGGTTTCCGCCGTGGCGGAGAAGAATTTATCGGTGAGCGCCAGTAAATGCTGTTGCCATTGTTCCATGGAATAACGGCTTTGCAGCGTTTGGAACCAATCAAATAACGCTGCTAAAAATGCCGCCAATTGCCCCGCCAGCTGCCCTTTTAAGCCGAAACTGTTATCCAGCCCGAGGCTGTCTTGCCAAATGCCGTTTTCTTCACGCAAGGCATAGCCCAACAACATTCGCTCCAAGcc                                                                                                                                                                                                                                      <  1:566443/251‑1 (MQ=255)                  gTTTCATCGGTTTCCGCCGTGGCGGAGAAGAATTTATCGGTGAGCGCCAGTAAATGCTGTTGCCATTGTTCCATGGAATAACGGCTTTGCAGCGTTTGGAACCAATCAAATAACGCTGCTAAAAATGCCGCCAATTGCCCCGCCAGCTGCCCTTTTAAGCCGAAACTGTTATCCAGCCCGAGGCTGTCTTGCCAAATGCCGTTTTCTTCACGCAAGGCATAGCCCAACAACATTCGCTCCAAGcccgc                                                                                                                                                                                                                                   >  1:625939/1‑245 (MQ=255)                     tCATCGGTTTCCGCCGTGGCGGAGAAGAATTTATCGGTGAGCGCCAGTAAATGCTGTTGCCATTGTTCCATGGAATAACGGCTTTGCAGCGTTTGGAACCAATCAAATAACGCTGCTAAAAATGCCGCCAATTGCCCCGCCAGCTGCCCTTTTAAGCCGAAACTGTTATCCAGCCCGAGGCTGTCTTGCCAAATGCCGTTTTCTTCACGCAAGGCATAGCCCAACAACATTCGCTCCAAGCCCGCCTGCCa                                                                                                                                                                                                                             >  1:323364/1‑251 (MQ=255)                           gTTTCCGCCGTGGCGGAGAAGAATTTATCGGTGAGCGCCAGTAAATGCTGTTGCCATTGTTCCATGGAATAACGGCTTTGCAGCGTTTGGAACCAATCAAATAACGCTGCTAAAAATGCCGCCAATTGCCCCGCCAGCTGCCCTTTTAAGCCGAAACTGTTATCCAGCCCGAGGCTGTCTTGCCAAATGCCGTTTTCTTCACGCAAGGCATAGCCCAACAACATTCGCTCCAAGCCCGCCTGCCATGAAt                                                                                                                                                                                                                        <  1:115079/250‑1 (MQ=255)                               ccgccgTGGCGGAGAAGAATTTATCGGTGAGCGCCAGTAAATGCTGTTGCCATTGTTCCATGGAATAACGGCTTTGCAGCGTTTGGAACCAATCAAATAACGCTGCTAAAAATGCCGCCAATTGCCCCGCCAGCTGCCCTTTTAAGCCGAAACTGTTATCCAGCCCGAGGCTGTCTTGCCAAATGCCGTTTTCTTCACGCAAGGCATAGCCCAACAACATTCGCTCCAAGCCCGCCTGCCATGAATTGTAg                                                                                                                                                                                                                   <  1:646285/251‑1 (MQ=255)                                  ccgtggcggagaagaaTTTATCGGTGAGCGCCAGTAAATGCTGTTGCCATTGTTCCATGGAATAACGGCTTTGCAGCGTTTGGAACCAATCAAATAACGCTGCTAAAAATGCCGCCAATTGCCCCGCCAGCTGCCCTTTTAAGCCGAAACTGTTATCCAGCCCGAGGCTGTCTTGCCAAATGCCGTTTTCTTCACGCAAGGCATAGCCCAACAACATTCGCTCCAAGCCCGCCTGCCATGAATTGTAGTTa                                                                                                                                                                                                                <  1:654118/241‑1 (MQ=255)                                                    tATCGGTGAGCGCCAGTAAATGCTGTTGCCATTGTTCCATGGAATAACGGCTTTGCAGCGTTTGGAACCAATCAAATAACGCTGCTAAAAATGCCGCCAATTGCCCCGCCAGCTGCCCTTTTAAGCCGAAACTGTTATCCAGCCCGAGGCTGTCTTGCCAAATGCCGTTTTCTTCACGCAAGGCATAGCCCAACAACATTCGCTCCAAGCCCGCCTGCCATGAATTGTAGTTACTTTCCGCTTCGTCAttt                                                                                                                                                                                              <  1:839993/251‑1 (MQ=255)                                                         gTGAGCGCCAGTAAATGCTGTTGCCATTGTTCCATGGAATAACGGCTTTGCAGCGTTTGGAACCAATCAAATAACGCTGCTAAAAATGCCGCCAATTGCCCCGCCAGCTGCCCTTTTAAGCCGAAACTGTTATCCAGCCCGAGGCTGTCTTGCCAAATGCCGTTTTCTTCACGCAAGGCATAGCCCAACAACATTCGCTCCAAGcc                                                                                                                                                                                                                                      >  1:14466/1‑206 (MQ=255)                                                                ccAGTAAATGCTGTTGCCATTGTTCCATGGAATAACGGCTTTGCAGCGTTTGGAACCAATCAAATAACGCTGCTAAAAATGCCGCCAATTGCCCCGCCAGCTGCCCTTTTAAGCCGAAACTGTTATCCAGCCCGAGGCTGTCTTGCCAAATGCCGTTTTCTTCACGCAAGGCATAGCCCAACAACATTCGCTCCAAGCCCGCCTGCCATGAATTGTAGTTACTTTCCGCTTCGTCATTTTTTGCTAAGCCg                                                                                                                                                                                  >  1:190298/1‑251 (MQ=255)                                                                  aGTAAATGCTGTTGCCATTGTTCCATGGAATAACGGCTTTGCAGCGTTTGGAACCAATCAAATAACGCTGCTAAAAATGCCGCCAATTGCCCCGCCAGCTGCCCTTTTAAGCCGAAACTGTTATCCAGCCCGAGGCTGTCTTGCCAAATGCCGTTTTCTTCACGCAAGGCATAGCCCAACAACATTCGCTCCAAGCCCGCCTGCCATGAATTGTAGTTACTTTCCGc                                                                                                                                                                                                        <  1:691953/227‑1 (MQ=255)                                                                                     gTTCCATGGAATAACGGCTTTGCAGCGTTTGGAACCAATCAAATAACGCTGCTAAAAATGCCGCCAATTGCGCCGCCAGCTGCCCTTTTAAGCCGAAACTGTTATCCAGCCCGAGGCTGTCTTGCCAAATGCCGTTTTCTTCACGCAAGGCATAGCCCAACAACATTCGCTCCAAGCCCGCCTGCCATGAATTGTAGTTACTTTCCGCTTCGTCATTTTTTGCTAAGCCGAAACGCACGCCGGCAttttt                                                                                                                                                              >  1:439682/1‑250 (MQ=255)                                                                                     gTTCCATGGAATAACGGCTTTGCAGCGTTTGGAACCAATCAAATAACGCTGCTAAAAATGCCGCCAATTGCCCCGCCAGCTGCCCTTTTAAGCCGAAACTGTTATCCAGCCCGAGGCTGTCTTGCCAAATGCCGTTTTCTTCACGCAAGGCATAGCCCAACAACATTCGCTCCAAGCCCGCCTGCCATGAATTGTAGTTACTTTCCGCTTCGTCATTTTTTGCTAAGCCGAAACGCACGCCGGCAttttt                                                                                                                                                              >  1:380612/1‑250 (MQ=255)                                                                                                               gTTTGGAACCAATCAAATAACGCTGCTAAAAATGCCGCCAATTGCCCCGCCAGCTGCCCTTTTAAGCCGAAACTGTTATCCAGCCCGAGGCTGTCTTGCCAAATGCCGTTTTCTTCACGCAAGGCATAGCCCAACAACGTTCGCTCCAAGCCCGCCTGCCATGAATTGTAGTTACTTTCCGCTTCGTCATTTTTTGCTAAGCCGAAACGCACGCCGGCATTTTTCACCCAATCGCGAATTTGCGCCAAAt                                                                                                                                    >  1:11493/1‑250 (MQ=255)                                                                                                               gTTTGGAACCAATCAAATAACGCTGCTAAAAATGCCGCCAATTGCCCCGCCAGCTGCCCTTTTAAGCCGAAACTGTTATCCAGCCCGAGGCTGTCTTGCCAAATGCCGTTTTCTTCACGCAAGGCATAGCCCAACAACATTCGCTCCAAGCCCGCCTGCCATGAATTGTAGTTACt                                                                                                                                                                                                              <  1:246449/176‑1 (MQ=255)                                                                                                               gTTTGGAACCAATCAAATAACGCTGCTAAAAATGCCGCCAATTGCCCCGCCAGCTGCCCTTTTAAGCCGAAACTGTTATCCAGCCCGAGGCTGTCTTGCCAAATGCCGTTTTCTTCACGCAAGGCATAGCCCAACAACATTCGCTCCAAGCCCGCCTGCCATGAATTGTAGTTACTTTCCGCTTCGTCATTTTTTGCTAAGCCGAAACGCACGCCGGCATTTTTCACCCAATCGCGAATTTGCGCCAAAt                                                                                                                                    >  1:294515/1‑250 (MQ=255)                                                                                                               gTTTGGAACCAATCAAATAACGCTGCTAAAAATGCCGCCAATTGCCC‑GCCAGCTGCCCTTTTAAGCCGAAACTGTTATCCAGCCCGAGGCTGTCTTGCCAAATGCCGTTTTCTTCACGCAAGGCATAGCCCAACAACATTCGCTCCAAGCCCGCCTGCCATGAATTGTAGTTACTTTCCGCTTCGTCATTTTTTGCTAAGCCGAAACGCACGCCGGCATTTTTCACCCAATCGCGAATTTGCGCCAAATc                                                                                                                                   >  1:132815/1‑250 (MQ=255)                                                                                                                tttGGAACCAATCAAATAACGCTGCTAAAAATGCCGCCAATTGCCCCGCCAGCTGCCCTTTTAAGCCGAAACTGTTATCCAGCCCGAGGCTGTCTTGCCAAATGCCGTTTTCTTCACGCAAGGCATAGCCCAACAACATTCGCTCCAAGCCCGCCTGCCATGAATTGTAGTTACTTTCCGCTTCGTCATTTTTTGCTAAGCCGAAACGCACGCCGGCATTTTTCACCCAATCGCGAATTTGCGCCAAATc                                                                                                                                   >  1:404536/1‑250 (MQ=255)                                                                                                                tttGGAACCAATCAAATAACGCTGCTAAAAATGCCGCCAATTGCCCCGCCAGCTGCCCTTTTAAGCCGAAACTGTTATCCAGCCCGAGGCTGTCTTGCCAAATGCCGTTTTCTTCACGCAAGGCATAGCCCAACAACATTCGCTCCAAGCCCGCCTGCCATGAATTGTAGTTACTTTCCGCTTCGTCATTTTTTGCTAAGCCGAAACGCACGCCGGCATTTTTCACCCAATCGCGAATTTGCGCCAAATc                                                                                                                                   <  1:814041/250‑1 (MQ=255)                                                                                                                        caatcaaaTAACGCTGCTAAAAATGCCGCCAATTGCCCCGCCAGCTGCCCTTTTAAGCCGAAACTGTTATCCAGCCCGAGGCTGTCTTGCCAAATGCCGTTTTCTTCACGCAAGGCATAGCCCAACAACATTCGCTCCAAGCCCGCCTGCCATGAATTGTAGTTACTTTCCGCTTCGTCATTTTTTGCTAAGCCGAAACGCACGCCGGCATTTTTCACCCAATCGCGAATTTGCGCCAAATCCTGCAATTc                                                                                                                          <  1:776139/245‑1 (MQ=255)                                                                                                                            caaaTAACGCTGCTAAAAATGCCGCCAATTGCCCCGCCAGCTGCCCTTTTAAGCCGAAACTGTTATCCAGCCCGAGGCTGTCTTGCCAAATGCCGTTTTCTTCACGCAAGGCATAGCCCAACAACATTCGCTCCAAGCCCGCCTGCCATGAATTGTAGTTACTTTCCGCTTCGTCATTTTTTGCTAAGCCGAAACGCACGCCGGCATTTTTCACCCAATCGCGAATTTGCGCCAAATCCTGCAATTCAata                                                                                                                      >  1:357684/3‑251 (MQ=255)                                                                                                                             aaaTAACGCTGCTAAAAATGCCGCCAATTGCCCCGCCAGCTGCCCTTTTAAGCCGAAACTGTTATCCAGCCCGAGGCTGTCTTGCCAAATGCCGTTTTCTTCACGCAAGGCATAGCCCAACAACATTCGCTCCAAGCCCGCCTGCCATGAATTGTAGTTACTTTCCGCTTCGTCATTTTTTGCTAAGCCGAAACGCACGCCGGCATTTTTCACCCAATCGCGAATTTGCGCCAAATCCTGCAATTCAatat                                                                                                                     <  1:407042/250‑1 (MQ=255)                                                                                                                                  ccgctgcTAAAAATGCCGCCAATTGCCCCGCCAGCTGCCCTTTTAAGCCGAAACTGTTATCCAGCCCGAGGCTGTCTTGCCAAATGCCGTTTTCTTCACGCAAGGCATAGCCCAACAACATTCGCTCCAAGCCCGCCTGCCATGAATTGTAGTTACTTTCCGCTTCGTCATTTTTTGCTAAGCCGAAACGCACGCCGGCATTTTTCACCCAATCGCGAATTTGCGCCAAATCCTGCAATTCAATATTAAAg                                                                                                                <  1:440149/246‑1 (MQ=255)                                                                                                                                  aCGCTGCTAAAAATGCCGCCAATTGCCCCGCCAGCTGCCCTTTTAAGCCGAAACTGTTATCCAGCCCGAGGCTGTCTTGCCAAATGCCGTTTTCTTCACGCAAGGCATAGCCCAACAACATTCGCTCCAAGCCCGCCTGCCATGAATTGTAGTTACTTTCCGCTTCGTCATTTTTTGCTAAGCCGAAACGCACGCCGGCATTTTTCACCCAATCGCGAATTTGCGCCAAATCCTGCAATTCAATATTAAAg                                                                                                                <  1:801952/251‑1 (MQ=255)                                                                                                                                  aCGCTGCTAAAAATGCCGCCAATTGCCCCGCCAGCTGCCCTTTTAAGCCGAAACTGTTATCCAGCCCGAGGCTGTCTTGCCAAATGCCGTTTTCTTCACGCAAGGCATAGCCCAACAACATTCGCTCCAAGCCCGCCTGCCATGAATTGTAGTTACTTTCCGCTTCGTCATTTTTTGCTAAGCCGAAACGCACGCCGGCATTTTTCACCCAATCGCGAATTTGCGCCAAATCCTGCAATTCAATATTAAAg                                                                                                                <  1:452350/251‑1 (MQ=255)                                                                                                                                  aCGCTGCTAAAAATGCCGCCAATTGCCCCGCCAGCTGCCCTTTTAAGCCGAAACTGTTATCCAGCCCGAGGCTGTCTTGCCAAATGCCGTTTTCTTCACGCAAGGCATAGCCCAACAACATTCGCTCCAAGCCCGCCTGCCATGAATTGTAGTTACTTTCCGCTTCGTCATTTTTTGCTAAGCCGAAACGCACGCCGGCATTTTTCACCCAATCGCGAATTTGCGCCAAATCCTGCAATTCAATATTAAAg                                                                                                                <  1:443594/251‑1 (MQ=255)                                                                                                                                  aCGCTGCTAAAAATGCCGCCAATTGCCCCGCCAGCTGCCCTTTTAAGCCGAAACTGTTATCAAGCCCGAGGCTGTCTTGCCAAATGCCGTTTTCTTCACGCAAGGCATAGCCCAACAACATTCGCTCCAAGCCCGCCTGCCATGAATTGTAGTTACTTTCCGCTTCGTCATTTTTTGCTAAGCCGAAACGCACGCCGGCATTTTTCACCCAATCGCGAATTTGCGCCAAATCCTGCAATTCAATATTAAAg                                                                                                                <  1:319988/251‑1 (MQ=255)                                                                                                                                     ctgcTAAAAATGCCGCCAATTGCCCCGCCAGCTGCCCTTTTAAGCCGAAACTGTTATCCAGCCCGAGGCTGTCTTGCCAAATGCCGTTTTCTTCACGCAAGGCATAGCCCAACAACATTCGCTCCAAGCCCGCCTGCCATGAATTGTGGTTACTTTCCGCTTCGTCATTTTTTGCTAAGCCGAAACGCACGCCGGCATTTTTCACCCAATCGCGAATTTGCGCCAAATCCTGCAATTCAATATTAAAGCg                                                                                                              <  1:246877/248‑1 (MQ=255)                                                                                                                                       gcTAAAAATGCCGCCAATTGCCCCGCCAGCTGCCCTTTTAAGCCGAAACTGTTATCCAGCCCGAGGCTGTCTTGCCAAATGCCGTTTTCTTCACGCAAGGCATAGCCCAACAACATTCGCTCCAAGCCCGCCTGCCATGAATTGTAGTTACTTTCCGCTTCGTCATTTTTTGCTAGGCCGAAACGCACGCCGGCATTTTTCACCCAATCGCGAATTTGCGCCAAATCCTGCAATTCAATATTAAAGCGTTg                                                                                                           >  1:113912/1‑251 (MQ=255)                                                                                                                                       gcTAAAAATGCCGCCAATTGCCCCGCCAGCTGCCCTTTTAAGCCGAAACTGTTATCCAGCCCGAGGCTGTCTTGCCAAATGCCGTTTTCTTCACGCAAGGCATAGCCCAACAACATTCGCTCCAAGCCCGCCTGCCATGAATTGTAGTTACTTTCCGCTTCGTCATTTTTTGCTAAGCCGAAacg                                                                                                                                                                             >  1:360994/1‑185 (MQ=255)                                                                                                                                       gcTAAAAATGCCGCCAATTGCCCCGCCAGCTGCCCTTTTAAGCCGAAACTGTTATCCAGCCCGAGGCTGTCTTGCCAAATGCCGTTTTCTTCACGCAAGGCATAGCCCAACAACATTCGCTCCAAGCCCGCCTGCCATGAATTGTAGTTACTTTCCGCTTCGTCATTTTTTGCTAAGCCGAAACGCACGCCGGCATTTTTCACCCAATCGCGAATTTGCGCCAAATCCTGCAATTCAATATTAAAGCGTTg                                                                                                           >  1:468304/1‑251 (MQ=255)                                                                                                                                                             ccgCCAGCTGCCCTTTTAAGCCGAAACTGTTATCCAGCCCGAGGCTGTCTTGCCAAATGCCGTTTTCTTCACGCAAGGCATAGCCCAACAACATTCGCTCCAAGCCCGCCTGCCATGAATTGTAGTTACTTTCCGCTTCGTCATTTTTTGCTAAGCCGAAACGCACGCCGGCATTTTTCACCCAATCGCGAATTTGCGCCAAATCCTGCAATTCAATATTAAAGCGTTGGCGAATTGCCGGCACATCAAGc                                                                                     <  1:56062/249‑1 (MQ=255)                                                                                                                                                                      gCCCTTTTAAGCCGAAACTGTTATCCAGCCCGAGGCTGTCTTGCCAAATGCCGTTTTCTTCACGCAAGGCATAGCCCAACAACATTCGCTCCAAGCCCGCCTGCCATGAATTGTAGTTACTTTCCGCTTCGTCATTTTTTGCTAAGCCGAAACGCACGCCGGCATTTTTCACCCAATCGCGAATTTGCGCCAAATCCTGCAATTCAATATTAAAGCGTTGGCGAATTGCCGGCACATCAAGCAATGCCAg                                                                             >  1:860987/1‑250 (MQ=255)                                                                                                                                                                      gCCCTTTTAAGCCGAAACTGTTATCCAGCCCGAGGCTGTCTTGCCAAATGCCGTTTTCTTCACGCAAGGCATAGCCCAACAACATTCGCTCCAAGCCCGCCTGCCATGAATTGTAGTTACTTTCCGCTTCGTCATTTTTTGCTAAGCCGAAACGCACGCCGGCATTTTTCACCCAATCGCGAATTTGCGCCAAATCCTGCAATTCAATATTAAAGCGTTGGCGAATTGCCGGCACATCAAGCAATGCCAg                                                                             >  1:806617/1‑250 (MQ=255)                                                                                                                                                                        ccTTTTAAGCCGAACCTGTTATCCAGCCCGAGGCTGTCTTGCCAAATGCCGTTTTCTTCACGCAAGGCATAGCCCAACAACATTCGCTCCAAGCCCGCCTGCCATGAATTGTAGTTACTTTCCGCTTCGTCATTTTTTGCTAAGCCGAAACGCACGCCGGCATTTTTCACCCAATCGCGAATTTGCGCCAAATCCTGCAATTCAATATTAAAGCGTTGGCGAATTGCCGGCACATCAAGCAATGCCAGCAc                                                                          <  1:550280/251‑1 (MQ=255)                                                                                                                                                                                       cTGTTATCCAGCCCGAGGCTGTCTTGCCAAATGCCGTTTTCTTCACGCAAGGCATAGCCCAACAACATTCGCTCCAAGCCCGCCTGCCATGAATTGTAGTTACTTTCCGCTTCGTCATTTTTTGCTAAGCCGAAACGCACGCCGGCATTTTTCACCCAATCGCGAATTTGCGCCAAATCCTGCAATTCAATATTAAAGCg                                                                                                              <  1:128693/200‑1 (MQ=255)                                                                                                                                                                                       cTGTTATCCAGCCCGAGGCTGTCTTGCCAAATGCCGTTTTCTTCACGCAAGGCATAGCCCAACAACATTCGCTCCAAGCCCGCCTGCCATGAATTGTAGTTACTTTCCGCTTCGTCATTTTTTGCTAAGCCGAAACGCACGCCGGCATTTTTCACCCAATCGCGAATTTGCGCCAAATCCTGCAATTCAATATTAAAGCGTTGGCGAATTGCCGGCACATCAAGCAATGCCAGCACGTCTTCTGCACTaaa                                                           >  1:218062/1‑251 (MQ=255)                                                                                                                                                                                       cTGTTATCCAGCCCGAGGCTGTCTTGCCAAATGCCGTTTTCTTCACGCAAGGCATAGCCCAACAACATTCGCTCCAAGCCCGCCTGCCATGAATTGTAGTTACTTTCCGCTTCGTCATTTTTTGCTAAGCCGAAACGCACGCCGGCATTTTTCACCCAATCGCGAATTTGCGCCAAATCCTGCAATTCAATATTAAAGCGTTGGCGAATTGCCGGCACATCAAGCAATGCCAGCACGTCTTCTGCACTaaa                                                           >  1:135265/1‑251 (MQ=255)                                                                                                                                                                                         gTTATCCAGCCCGAGGCTGTCTTGCCAAATGCCGTTTTCTTCACGCAAGGCATAGCCCAACAACATTCGCTCCAAGCCCGCCTGCCATGAATTGTAGTTACTTTCCGCTTCGTCATTTTTTGCTAAGCCGAAACGCACGCCGGCATTTTTCACCCAATCGCGAATTTGCGCCAAATCCTGCAATTCAATATTAAAGCGTTGGCGAATTGCCGGCACATCAAGCAATGCCAGCACGTCTTCTGCACTAAACg                                                         >  1:202035/1‑251 (MQ=255)                                                                                                                                                                                            aTCCAGCCCGAGGCTGTCTTGCCAAATGCCGTTTTCTTCACGCAAGGCATAGCCCAACAACATTCGCTCCAAGCCCGCCTGCCATGAATTGTAGTTACTTTCCGCTTCGTCATTTTTTGCTAAGCCGAAACGCACGCCGGCATTTTTCACCCAATCGCGAATTTGCGCCCAATCCTGCAATTCAATATTAAAGCGTTGGCGAATTGCCGGCACATCAAGCa                                                                                    >  1:437681/1‑221 (MQ=255)                                                                                                                                                                                            aTCCAGCCCGAGGCTGTCTTGCCAAATGCCGTTTTCTTCACGCAAGGCATAGCCCAACAACATTCGCTCCAAGCCCGCCTGCCATGAATTGTAGTTACTTTCCGCTTCGTCATTTTTTGCTAAGCCGAAACGCACGCCGGCATTTTTCACCCAATCGCGAATTTGCGCCAAATCCTGCAATTCAATATTAAAGCGTTGGCGAATTGCCGGCACATCAAGCAATGCCAGCACGTCTTCTGCACTAAACGTGc                                                      <  1:595021/251‑1 (MQ=255)                                                                                                                                                                                                 gCCCGAGGCTGTCTTGCCAAATGCCGTTTTCTTCACGCAAGGCATAGCCCAACAACATTCGCTCCAAGCCTGCCTGCCATGAATTGTAGTTACTTTCCGCTTCGTCATTTTTTGCTAAGCCGAAACGCACGCCGGCATTTTTCACCCAATCGCGAATTTGCGCCAAATCCTGCAATTCAATATTAAAGCGTTGGCGAATTGCCGGCACATCAAGCAATGCCAGCACGTCTTCTGCACTaa                                                            <  1:431087/240‑1 (MQ=255)                                                                                                                                                                                                 gCCCGAGGCTGTCTTGCCAAATGCCGTTTTCTTCACGCAAGGCATAGCCCAACAACATTCGCTCCAAGCCCGCCTGCCATGAATTGTAGTTACTTTCCGCTTCGTCATTTTTTGCTAAGCCGAAACGCACGCCGGCATTTTTCACCCAATCGCGAATTTGCGCCAAATCCTGCAATTCAATATTAAAGCGTTGGCGAATTGCCGGCACATCAAGCa                                                                                    <  1:570946/216‑1 (MQ=255)                                                                                                                                                                                                 gCCCGAGGCTGTCTTGCCAAATGCCGTTTTCTTCACGCAAGGCATAGCCCAACAACATTCGCTCCAAGCCCGCCTGCCATGAATTGTAGTTACTTTCCGCTTCGTCATTTTTTGCTAAGCCGAAACGCACGCCGGCATTTTTCACCCAATCGCGAATTTGCGCCAAATCCTGCAATTCAATATTAAAGCGTTGGCGAATTGCCGGCACATCAAGCAATGCCAGCACGTCTTCTGCACTAAACGTGcttt                                                   >  1:161995/1‑249 (MQ=255)                                                                                                                                                                                                   ccGAGGCTGTCTTGCCAAATGCCGTTTTCTTCACGCAAGGCATAGCCCAACAACATTCGCTCCAAGCCCGCCTGCCATGAATTGTAGTTACTTTCCGCTTCGTCATTTTTTGCTAAGCCGAAACGCACGCCGGCATTTTTCACCCAATCGCGAATTTGCGCCAAATCCTGCAATTCAATATTAAAGCGTTGGCGAATTGCCGGCACATCAAGCAATGCCAGCACGTCTTCTGCACTAAACGTGctttcttt                                               <  1:788461/251‑1 (MQ=255)                                                                                                                                                                                                           gtcttGCCAAATGCCGTTTTCTTCACGCAAGGCATAGCCCAACAACATTCGCTCCAAGCCCGCCTGCCATGAATTGTAGTTACTTTCCGCTTCGTCATTTTTTGCTAAGCCGAAACGCACGCCGGCATTTTTCACCCAATCGCGAATTTGCGCCAAATCCTGCAATTCAATATTAAAGCGTTGGCg                                                                                                        <  1:153750/182‑1 (MQ=255)                                                                                                                                                                                                           gtcttGCCAAATGCCGTTTTCTTCACGCAAGGCATAGCCCAACAACATTCGCTCCAAGCCCGCCTGCCATGAATTGTAGTTACTTTCCGCTTCGTCATTTTTTGCTAAGCCGAAACGCACGCCGGCATTTTTCACCCAATCGCGAATTTGCGCCAAATCCTGCAATTCAATATTAAAGCGTTGGCGAATTGCCGGCACATCAAGCAATGCCAGCACGTCTTCTGCACTAAACGTGCTTTCTTTCAGCTGCa                                       >  1:299997/5‑251 (MQ=255)                                                                                                                                                                                                           gtcttGCCAAATGCCGTTTTCTTCACGCAAGGCATAGCCCAACAACATTCGCTCCAAGCCCGCCTGCCATGAATTGTAGTTACTTTCCGCTTCGTCATTTTTTGCTAAGCCGAAACGCACGCCGGCATTTTTCACCCAATCGCGAATTTGCGCCAAATCCTGCAATTCAATATTAAAGCGTTGGCGAATTGCCGGCACATCAAGCAATGCCAGCACGTCTTCTGCACTAAACGTGCTTTCTTTCAGCTGCa                                       >  1:721358/5‑251 (MQ=255)                                                                                                                                                                                                             cttGCCAAATGCCGTTTTCTTCACGCAAGGCATAGCCCAACAACATTCGCTCCAAGCCCGCCTGCCATGAATTGTAGTTACTTTCCGCTTCGTCATTTTTTGCTAAGCCGAAACGCACGCCGGCATTTTTCACCCAATCGCGAATTTGCGCCAAATCCTGCAATTCAATATTAAAGCGTTGGCGAATTGCCGGCACATCAAGCAATGCCAGCACGTCTTCTGCACTAAACGTGCTTTCTTTCAGCTGCAAt                                     <  1:146513/249‑1 (MQ=255)                                                                                                                                                                                                              ttGCCAAATGCCGTTTTCTTCACGCAAGGCATAGCCCAACAACATTCGCTCCAAGCCCGCCTGCCATGAATTGTAGTTACTTTCCGCTTCGTCATTTTTTGCTAAGCCGAAACGCACGCCGGCATTTTTCACCCAATCGCGAATTTGCGCCAAATCCTGCAATTCAATATTAAAGCGTTGGCGAATTGCCGGCACATCAAGCAATGCCAGCACGTCTTCTGCACTAAACGTGCTTTCTTTCAGCTGCAATa                                    <  1:801318/250‑1 (MQ=255)                                                                                                                                                                                                               tGCCAAATGCCGTTTTCTTCACGCAAGGCATAGCCCAACAACATTCGCTCCAAGCCCGCCTGCCATGAATTGTAGTTACTTTCCGCTTCGTCATTTTTTGCTAAGCCGAAACGCACGCCGGCATTTTTCACCCAATCGCGAATTTGCGCCAAATCCTGCAATTCAATATTAAAGCGTTGGCGAATTGCCGGCACATCAAGCAATGCCAGCACGTCTTCTGCACTAAACGTGCTTTCTTTCAGCTGCAATaa                                   <  1:571060/251‑1 (MQ=255)                                                                                                                                                                                                                 ccAAATGCCGTTTTCTTCACGCAAGGCATAGCCCAACAACATTCGCTCCAAGCCCGCCTGCCATGAATTGTAGTTACTTTCCGCTTCGTCATTTTTTGCTAAGCCGAAACGCACGCCGGCATTTTTCACCCAATCGCGAATTTGCGCCAAATCCTGCAATTCAATATTAAAGCGTTGGCGAATTGCCGGCACATCAAGCAATGCCAGCACGTCTTCTGCACTAAACGTGCTTTCTTTCAGCTGCAATAAgg                                 <  1:574765/251‑1 (MQ=255)                                                                                                                                                                                                                       gCCGTTTTCTTCACGCAAGGCATAGCCCAACAACATTCGCTCCAAGCCCGCCTGCCATGAATTGTAGTTACTTTCCGCTTCGTCATTTTTTGCTAAGCCGAAACGCACGCCGGCATTTTTCACCCAATCGCGAATTTGCGCCAAATCCTGCAATTCAATATTAAAGCGTTGGCGAATTGCCGGCACATCAAGCAATGCCAGCACGTCTTCTGCACTAAACGTGCTTTCTTTCAGCTGCAATAAGGTCAGaa                           >  1:152891/1‑251 (MQ=255)                                                                                                                                                                                                                        ccGTTTTCTTCACGCAAGGCATAGCCCAACAACATTCGCTCCAAGCCCGCCTGCCATGAATTGTAGTTACTTTCCGCTTCGTCATTTTTTGCTAAGCCGAAACGCACGCCGGCATTTTTCACCCAATCGCGAATTTGCGCCAAATCCTGCAATTCAATATTAAAGCGTTGGCGAATTGCCGGCACATCAAGCAATGCCAGCACGTCTTCTGCACTAAACGTGCTTTCTTTCAGCTGCAATAAGGTCAGaaa                          <  1:865048/251‑1 (MQ=255)                                                                                                                                                                                                                           ttttCTTCACGCAAGGCATAGCCCAACAACATTCGCTCCAAGCCCGCCTGCCATGAATTGTAGTTACTTTCCGCTTCGTCATTTTTTGCTAAGCCGAAACGCACGCCGGCATTTTTCACCCAATCGCGAATTTGCGCCAAATCCTGCAATTCAATATTAAAGCGTTGGCGAATTGCCGGCACATCAAGCAATGCCAGCACGTCTTCTGCACTAAACGTGCTTTCTTTCAGCTGCAATAAGGTCAGAAAAc                        <  1:180665/250‑1 (MQ=255)                                                                                                                                                                                                                                 tcacgcAAGGCATAGCCCAACAACATTCGCTCCAAGCCCGCCTGCCATGAATTGTAGTTACTTTCCGCTTCGTCATTTTTTGCTAAGCCGAAACGCACGCCGGCATTTTTCACCCAATCGCGAATTTGCGCCAAATCCTGCAATTCAATATTAAAGCGTTGGCGAATTGCCGGCACATCAAGCAATGCCAGCACGTCTTCTGCACTAAACGTGCTTTCTTTCAGCTGCAATAAGGTCAGAAAACCGGCGAt                 >  1:569349/4‑251 (MQ=255)                                                                                                                                                                                                                                   acgcAAGGCATAGCCCAACAACATTCGCTCCAAGCCCGCCTGCCATGAATTGTAGTTACTTTCCGCTTCGTCATTTTTTGCTAAGCCGAAACGCACGCCGGCATTTTTCACCCAATCGCGAATTTGCGCCAAATCCTGCAATTCAATATTAAAg                                                                                                                <  1:337541/153‑1 (MQ=255)                                                                                                                                                                                                                                         ggCATAGCCCAACAACATTCGCTCCAAGCCCGCCTGCCATGAATTGTAGTTACTTTCCGCTTCGTCATTTTTTGCTAAGCCGAAACGCACGCCGGCATTTTTCACCCAATCGCGAATTTGCGCCAAATCCTGCAATTCAATATTAAAGCGTTGGCGAATTGCCGGCACATCAAGCAATGCCAGCACGTCTTCTGCAc                                                               >  1:640257/1‑197 (MQ=255)                                                                                                                                                                                                                                          gCATAGCCCAACAACATTCGCTCCAAGCCCGCCTGCCATGAATTGTAGTTACTTTCCGCTTCGTCATTTTTTGCTAAGCCGAAACGCACGCCGGCATTTTTCACCCAATCGCGAATTTGCGCCAAATCCTGCAATTCAATATTAAAGCGTTGGCGAATTGCCGGCACATCAAGCAATGCCAGCACGTCTTCTGCACTAAACGTGCTTTCTTTCAGCTGCAATAAGGTCAGAAAACCGGCGATAATcacgtt        >  1:126225/1‑248 (MQ=255)                                                                                                                                                                                                                                               gcccAACAACATTCGCTCCAAGCCCGCCTGCCATGAATTGTAGTTACTTTCCGCTTCGTCATTTTTTGCTAAGCCGAAACGCACGCCGGCATTTTTCACCCAATCGCGAATTTGCGCCAAATCCTGCAATTCAATATTAAAGCGTTGGCGAATTGCCGGCACATCAAGCAATGCCAGCACGTCTTctgc                                                                 >  1:619115/2‑186 (MQ=255)                                                                                                                                                                                                                                               gcccAACAACATTCGCTCCAAGCCCGCCTGCCATGAATTGTAGTTACTTTCCGCTTCGTCATTTTTTGCTAAGCCGAAACGCACGCCGGCATTTTTCACCCAATCGCGAATTTGCGCCAAATCCTGCAATTCAATATTAAAGCGTTGGCGAATTGCCGGCACATCAAGCAATGCCAGCACGTCTTCTGCACTAAACGTGCTTTCTTTCAGCTGCAATAAGGTCAGAAAACCGGCGATAATCACgttatt     >  1:70469/2‑249 (MQ=255)                                                                                                                                                                                                                                               gcccAACAACATTCGCTCCAAGCCCGCCTGCCATGAATTGGAGTTACTTTCCGCTTCGTCATTTGTTGCTAAGCCGAAACGCACGCCGGCATTTTTCACCCAATCGCGAATTTTCGCCAAATCCTGCAATTCAATATTAAAGCGGTGGCGAATTGCCGGCACATCAAGCAATGCCAGCACGTCTTCTGCACTAAACGGGct                                                     >  1:497005/2‑201 (MQ=255)                                                                                                                                                                                                                                                cccAACAACATTCGCTCCAAGCCCGCCTGCCATGAATTGTAGTTACTTTCCGCTTCGTCATTTTTTGCTAAGCCGAAACGCACGCCGGCATTTTTCACCCAATCGCGAATTTGCGCCAAATCCTGCAATTCAATATTAAAGCGTTGGCGAATTGCCGGCACATCAAGCAATGCCAGCACGTCTTCTGCACTAAACGTGCTTTCTTTCAGCTGCAATAAGGTCAGAAAACCGGCGATAATCACGTTATtctc  <  1:566190/251‑1 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                      CTTTAATATAAAACAGGGTTTCATCGGTTTCCGCCGAGGAGGCGAAGAATTTATCGGTGAGCGCCAGTAAATGCTGTTGCCATTGTTCCACGGAATAACGGTTTTGCAGCGTTTGGAACCAGTCGAATAACGCCGCTAAAAATGCCGCCAATTGCCCTGCCAGTTGCCCTTTCAAACCGAAACTGTTATCCAGCCCGAGGCTATCCTGCCAAATGCCGTTTTCTTCGCGCAAGGCATAACCCAATAACATTCGCTCCAAGCCGGCTTGCCATGAATTGTAGTTACTTTCCGCCTCATCATTTTTTGCTAAGCCGAAACGCACGCCGGCATTTTTCACCCAATCACGAATTTGCGCCAAATCCTGCAATTCAATATTAAAGCGTTGGCGAATTGCCGGCACATCAAGCAATGCCAGCACGTCTTCGGCACTAAACGTGCTTTCTTTCAGCTGCAATAAGGTCAGAAAACCGGCGATAATCACATTATTCTC  >  1/1989797‑1990286

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑