Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,989,960	T→C	Q576Q (CAA→CAG)	unknown ←	Exodeoxyribonuclease V gamma chain (EC 3.1.11.5)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,989,960	0	T	C	100.0%	194.4 / NA	68	Q576Q (CAA→CAG)	unknown	Exodeoxyribonuclease V gamma chain (EC 3.1.11.5)
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (34/34);  total (34/34)

TGACATCCGCCACCACATCCACGCCCAACGCGTCGCTAAAATGCACCTCCGCTAACTGTTCGGCAAATTGTCGGATTACCTCTTTAATATAAAACAGGGTTTCATCGGTTTCCGCCGAGGAGGCGAAGAATTTATCGGTGAGCGCCAGTAAATGCTGTTG‑CCATTGTTCCACGGAATAACGGTTTTGCAGCGTTTGGAACCAGTCGAATAACGCCGCTAAAAATGCCGCCAATTGCCCTGCCAGTTGCCCTTTCAAACCGAAACTGTTATCCAGCCCGAGGCTATCCTGCCAAATGCCGTTTTCTTCGCGCAAGGCATAACCCAATAACATTCGCTCCAAGCCGGCTTGCCATGAATTGTAGTTACTTTCCGCCTCATCATTTTTTGCTAAGCCGAAACGCACGCCGGCATTTTTCACCCAATCACGAATTTGCGCCAAATCCTGCAATTCAATATTAAAGCGTTGGCGAATTGCCGGCACATCAAGC  >  1/1989716‑1990203                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                    tGACATCTGCCACCACATCCACGCCCAACGCGTCGTTAAAATGCACCTCCGCTAACTGTTCGGCAAATTGCTGGATCACCTCTTTAATATAAAACAGGGTTTCATCGGTTTCCGCCGTGGCGGAGAAGAATTTATCGGTGAGCGCCAGTAAATGCTGTTG‑CCATTGTTCCATGGAATAACGGCTTTGCAGCGTTTGGAACCAATCAAATAACGCTGCTAAAAATGCCGCCAATTGCCCCGCCAGCTGcc                                                                                                                                                                                                                                                 >  1:870207/1‑249 (MQ=255)          ccaccaCATCCACGCCCAACGCGTCGTTAAAATGCACCTCCGCTAACTGTTCGGCAAATTGCTGGATCACCTCTTTAATATAAAACAGGGTTTCATCGGTTTCCGCCGTGGCGGAGAAGAATTTATCGGTGAGCGCCAGTAAATGCTGTTG‑CCATTGTTCCATGGAATAACGGCTTTGCAGCGTTTGGAACCAATCAAATAACGCTGCTAAAAATGCCGCCAATTGCCCCGCCAGCTGCCCttttaagccg                                                                                                                                                                                                                                      >  1:518631/1‑244 (MQ=255)                        cccAACGCGTCGTTAAAATGCACCTCCGCTAACTGTTCGGCAAATTGCTGGATCACCTCTTTAATATAAAACAGGGTTTCATCGGTTTCCGCCGTGGCGGAGAAGAATTTATCGGTGAGCGCCAGTAAATGCTGTTG‑CCATTGTTCCATGGAATAACGGCTTTGCAGCGTTTGGAACCAATCAAATAACGCTGCTAAAAATGCCGCCAATTGCCCCGCCAGCTGCCCTTTTAAGCCGAAACTGTTATCCAg                                                                                                                                                                                                                        >  1:896959/1‑251 (MQ=255)                                          tGCACCTCCGCTAACTGTTCGGCAAATTGCTGGATCACCTCTTTAATATAAAACAGGGTTTCATCGGTTTCCGCCGTGGCGGAGAAGAATTTATCGGTGAGCGCCAGTAAATGCTGTTG‑CCATTGTTCCATGGAATAACGGCTTTGCAGCGTTTGGAACCAATCAAATAACGCTGCTAAAAATGCCGCCAATTGCCCCGCCAGCTGCCCTTTTAAGCCGAAACTGTTATCCAGCCCGAGGCTGTCTTGCCa                                                                                                                                                                                                      <  1:763171/251‑1 (MQ=255)                                           gCACCTCCGCTAACTGTTCGGCAAATTGCTGGATCACCTCTTTAATATAAAACAGGGTTTCATCGGTTTCCGCCGTGGCGGAGAAGAATTTATCGGTGAGCGCCAGTAAATGCTGTTG‑CCATTGTTCCATGGAATAACGGCTTTGCAGCGTTTGGAACCAATCAAATAACGCTGCTAAAAATGCCGCCAATTGCCCCGCCAGCTGCCCTTTTAAGCCGAAACTGTTATCCAGCCCGAGGCTGTCTTGCCaa                                                                                                                                                                                                     >  1:568036/1‑251 (MQ=255)                                            cACCTCCGCTAACTGTTCGGCAAATTGCTGGATCACCTCTTTAATATAAAACAGGGTTTCATCGGTTTCCGCCGTGGCGGAGAAGAATTTATCGGTGAGCGCCAGTAAATGCTGTTG‑CCATTGTTCCATGGAAT‑ACGGCTTTGCAGCGTTTGGAACCAATCAAATAACGCTGCTAAAAATGCCGCCAATTGCCCCGCCAGCTGCCCTTTTAAGCCGAAACTGTTATCCAGCCCGAGGCTGTCTTGCCAAAt                                                                                                                                                                                                   >  1:464813/1‑251 (MQ=255)                                                tCCGCTAACTGTTCGGCAAATTGCTGGATCACCTCTTTAATATAAAACAGGGTTTCATCGGTTTCCGCCGTGGCGGAGAAGAATTTATCGGTGAGCGCCAGTAAATGCTGTTG‑CCATTGTTCCATGGAATAACGGCTTTGCAGCGTTTGGAACCAATCAAATAACGCTGCTAAAAATGCCGCCAATTGCCCCGCCAGCTGCCCTTTTAAGCCGAAACTGTTATCCAGCCCGAGGCTGTCTTGCCa                                                                                                                                                                                                      >  1:142744/1‑245 (MQ=255)                                                tCCGCTAACTGTTCGGCAAATTGCTGGATCACCTCTTTAATATAAAACAGGGTTTCATCGGTTTCCGCCGTGGCGGAGAAGAATTTATCGGTGAGCGCCAGTAAATGCTGTTG‑CCATTGTTCCATGGAATAACGGCTTTGCAGCGTTTGGAACCAATCAAATAACGCTGCTAAAAATGCCGCCAATTGCCCCGCCAGCTGCCCTTTTAAGCCGAAACTGTTATCCAGCCCGAGGCTGTCTTGCCAAATGc                                                                                                                                                                                                 >  1:539772/1‑250 (MQ=255)                                                 ccGCTAACTGTTCGGCAAATTGCTGGATCACCTCTTTAATATAAAACAGGGTTTCATCGGTTTCCGCCGTGGCGGAGAAGAATTTATCGGTGAGCGCCAGTAAATGCTGTTG‑CCATTGTTCCATGGAATAACGGCTTTGCAGCGTTTGGAACCAATCAAATAACGCTGCTAAAAATGCCGCCAATTGCCCCGCCAGCTGCCCTTTTAAGCCGAAACTGTTATcc                                                                                                                                                                                                                          <  1:352391/224‑1 (MQ=255)                                                 ccGCTAACTGTTCGGCAAATTGCTGGATCACCTCTTTAATATAAAACAGGGTTTCATCGGTTTCCGCCGTGGCGGAGAAGAATTTATCGGTGAGCGCCAGTAAATGCTGTTG‑CCATTGTTCCATGGAATAACGGCTTTGCAGCGTTTGGAACCAATCAAATAACGCTGCTAAAAATGCCGCCAATTGCCCCGCCAGCTGCCCTTTTAAGCCGAAACTGTTATCCAGCCCGAGGCTGTCTTGCCAAATGCCg                                                                                                                                                                                               >  1:603449/1‑251 (MQ=255)                                                                  aattgctggATCACCTCTTTAATATAAAACAGGGTTTCATCGGTTTCCGCCGTGGCGGAGAAGAATTTATCGGCGAGCGCCAGTAAATGCTGTTG‑CCATTGTTCCATGGAATAACGGCTTTGCAGCGTTTGGAACCAATCAAATAACGCTGCTAAAAATGCCGCCAATTGCCCCGCCAGCTGCCCTTTTAAGCCGAAACTGTTATCCAGCCCGAGGCTGTCTTGCCAAATGCCGTTTTCTTCACGCAAGGc                                                                                                                                                                              <  1:546203/244‑1 (MQ=255)                                                                      gctggATCACCTCTTTAATATAAAACAGGGTTTCATCGGTTTCCGCCGTGGCGGAGAAGAATTTATCGGTGAGCGCCAGTAAATGCTGTTG‑CCATTGTTCCATGGAATAACGGCTTTGCAGCGTTTGGAACCAATCAAATAACGCTGCTAAAAATGCCGCCAATTGCCCCGCCAGCTGCCCTTTTAAGCCGAAACTGTTATCCAGCCCGAGGCTGTCTTGCCAAATGCCGTTTTCTTCACGCAAGGCATag                                                                                                                                                                          >  1:74938/4‑250 (MQ=255)                                                                        tggATCACCTCTTTAATATAAAACAGGGTTTCATCGGTTTCCGCCGTGGCGGAGAAGAATTTATCGGTGAGCGCCAGTAAATGCTGTTG‑CCATTGTTCCATGGAATAACGGCTTTGCAGCGTTTGGAACCAATCAAATAACGCTGCTAAAAATGCCGCCAATTGCCCCGCCAGCTGCCCTTTTAAGCCGAAACTGTTATCCAGCCCGAGGCTGTCTTGCCAAATGCCGTTTTCTTCACGCAAGGCATagcc                                                                                                                                                                        <  1:85432/250‑4 (MQ=255)                                                                          gatcaCCTCTTTAATATAAAACAGGGTTTCATCGGTTTCCGCCGTGGCGGAGAAGAATTTATCGGTGAGCGCCAGTAAATGCTGTTG‑CCATTGTTCCATGGAATAACGGCTTTGCAGCGTTTGGAACCAATCAAATAACGCTGCTAAAAATGCCGCCAATTGCCCCGCCAGCTGCCCTTTTAAGCCGAAACTGTTATCCAGCCCGAGGCTGTCTTGCCAAATGCCGTTTTCTTCACGCAAGGCATAGccc                                                                                                                                                                       <  1:684274/246‑1 (MQ=255)                                                                              aCCTCTTTAATATAAAACAGGGTTTCATCGGTTTCCGCCGTGGCGGAGAAGAATTTATCGGTGAGCGCCAGTAAATGCTGTTG‑CCATTGTTCCATGGAATAACGGCTTTGCAGCGTTTGGAACCAATCAAATAACGCTGCTAAAAATGCCGCCAATTGCCCCGCCAGCTGCCCTTTTAAGCCGAAACTGTTATCCAGCCCGAGGCTGTCTTGCCAAATGCCGTTTTCTTCACGCAAGGCATAGCCCaacaa                                                                                                                                                                  <  1:705627/251‑4 (MQ=255)                                                                               ccTCTTTAATATAAAACAGGGTTTCATCGGTTTCCGCCGTGGCGGAGAAGAATTTATCGGTGAGCGCCAGTAAATGCTGTTGCCCATTGTTCCATGGAATAACGGCTTTGCAGCGTTTGGAACCAATCAAATAACGCTGCTAAAAATGCCGCCAATTGCCCCGCCAGCTGCCCTTTTAAGCCGAAACTGTTATCCAGCCCGAGGCTGTCTTGCCAAATGCCGTTTTCTTCACGCAAGGCATagcc                                                                                                                                                                        >  1:762790/1‑242 (MQ=255)                                                                               ccTCTTTAATATAAAACAGGGTTTCATCGGTTTCCGCCGTGGCGGAGAAGAATTTATCGGTGAGCGCCAGTAAATGCTGTTG‑CCATTGTTCCATGGAATAACGGCTTTGCAGCGTTTGGAACCAATCAAATAACGCTGCTAAAAATGCCGCCAATTGCCCCGCCAGCTGCCCTTTTAAGCCGAAACTGTTATcc                                                                                                                                                                                                                          <  1:48554/194‑1 (MQ=255)                                                                               ccTCTTTAATATAAAACAGGGTTTCATCGGTTTCCGCCGTGGCGGAGAAGAATTTATCGGTGAGCGCCAGTAAATGCTGTTG‑CCATTGTTCCATGGAATAACGGCTTTGCAGCGTTTGGAACCAATCAAATAACGCTGCTAAAAATGCCGCCAATTGCCCCGCCAGCTGCCCTTTTAAGCCGAAACTGTTATCCAGCCCGAGGCTGTCTTGCCAAATGCCGTTTTCTTCACGCAAGGCATAGCCCaacaa                                                                                                                                                                  <  1:887714/250‑4 (MQ=255)                                                                               ccTCTTTAATATAAAACAGGGTTTCATCGGTTTCCGCCGTGGCGGAGAAGAATTTATCGGTGAGCGCCAGTAAATGCTGTTG‑CCATTGTTCCATGGAATAACGGCTTTGCAGCGTTTGGAACCAATCAAATAACGCTGCTAAAAATGCCGCCAATTGCCCCGCCAGCTGCCCTTTTAAGCCGAAACTGTTATACAGCCCGAGGCTGTCTTGCCAAATGCCGTTTTCTTCACGCAAGGCATAGCCCaacaa                                                                                                                                                                  >  1:580066/1‑247 (MQ=255)                                                                                  ctTTAATATAAAACAGGGTTTCATCGGTTTCCGCCGTGGCGGAGAAGAATTTATCGGTGAGCGCCAGTGAATGCTGTTG‑CCATTGTTCCATGGAATAACGGCTTTGCAGCGTTTGGAACCAATCAAATAACGCTGCTAAAAATGCCGCCAATTGCCCCGCCAGCTGCCCTTTTAAGCCGAAACTGTTATCCAGCCCGAGGCTGTCTTGCCAAATGCCGTTTTCTTCACGCAAGGCATAGCCCAACAACAtt                                                                                                                                                              >  1:89547/1‑251 (MQ=255)                                                                                  ctTTAATATAAAACAGGGTTTCATCGGTTTCCGCCGTGGCGGAGAAGAATTTATCGGTGAGCGCCAGTAAATGCTGTTG‑CCATTGTTCCATGGAATAACGGCTTTGCAGCGTTTGGAACCAATCAAATAACGCTGCTAAAAATGCCGCCAATTGCCCCGCCAGCTGCCCttttaagc                                                                                                                                                                                                                                        <  1:895172/177‑6 (MQ=255)                                                                                  ctTTAATATAAAACAGGGTTTCATCGGTTTCCGCCGTGGCGGAGAAGAATTTATCGGTGAGCGCCAGTAAATGCTGTTG‑CCATTGTTCCATGGAATAACGGCTTTGCAGCGTTTGGAACCAATCAAATAACGCTGCTAAAAATGCCGCCAATTGCCCCGCCAGCTGCCCTTTTAAGCCGAAACTGTTATCCAGCCCGAGGCTGTCTTGCCa                                                                                                                                                                                                      <  1:156334/211‑1 (MQ=255)                                                                                  ctTTAATATAAAACAGGGTTTCATCGGTTTCCGCCGTGGCGGAGAAGAATTTATCGGTGAGCGCCAGTAAATGCTGTTG‑CCATTGTTCCATGGAATAACGGCTTTGCAGCGTTTGGAACCAATCAAATAACGCTGCTAAAAATGCCGCCAATTGCCCCGCCAGCTGCCCTTTTAAGCCGAAACTGTTATCCAGCCCGAGGCTGTCTTGCCAAATGCCGTTTTCTTCACGCAAGGCa                                                                                                                                                                             <  1:130040/236‑1 (MQ=255)                                                                                  ctTTAATATAAAACAGGGTTTCATCGGTTTCCGCCGTGGCGGAGAAGAATTTATCGGTGAGCGCCAGTAAATGCTGTTG‑CCATTGTTCCATGGAATAACGGCTTTGCAGCGTTTGGAACCAATCAAATAACGCTGCTAAAAATGCCGCCAATTGCCCCGCCAGCTGCCCTTTTAAGCCGAAACTGTTATCCAGCCCGAGGCTGTCTTGCCAAATGCCGTTTTCTTCACGCAAGGCATAGCCCAACAACAtt                                                                                                                                                              >  1:440998/1‑251 (MQ=255)                                                                                  ctTTAATATAAAACAGGGTTTCATCGGTTTCCGCCGTGGCGGAGAAGAATTTATCGGTGAGCGCCAGTAAATGCTGTTG‑CCATTGTTCCATGGAATAACGGCTTTGCAGCGTTTGGAACCAATCAAATAACGCTGCTAAAAATGCCGCCAATTGCCCCGCCAGCTGCCCTTTTAAGCCGAAACTGTTATCCAGCCCGAGGCTGTCTTGCCAAATGCCGTTTTCTTCACGCAAGGCATAGCCCAACAACAtt                                                                                                                                                              >  1:876212/1‑251 (MQ=255)                                                                                  ctTTAATATAAAACAGGGTTTCATCGGTTTCCGCCGTGGCGGAGAAGAATTTATCGGTGAGCGCCAGTAAATGCTGTTG‑CCATGGTTCCATGGAATAACGGCTTTGCAGCGTTTGGAACCAATCAAATAACGCTGCTAAAAATGCCGCCAATTGCCCCGCCAGCTGCCCTTTTAAGCCGAAACTGTTATCCAGCCCGAGGCTGTCTTGCCAAATGCCGTTTTCTTCACGCAAGGCATAGCCCaacaac                                                                                                                                                                 <  1:404094/248‑1 (MQ=255)                                                                                       atatAAAACAGGGTTTCATCGGTTTCCGCCGTGGCGGAGAAGAATTTATCGGTGAGCGCCAGTAAATGCTGTTG‑CCATTGTTCCATGGAATAACGGCTTTGCAGCGTTTGGAACCAATCAAATAACGCTGCTAAAAATGCCGCCAATTGCCCCGCCAGCTGCCCTTTTAAGCCGAAACTGTTATCCAGCCCGAGGc                                                                                                                                                                                                                >  1:145219/1‑196 (MQ=255)                                                                                       atatAAAACAGGGTTTCATCGGTTTCCGCCGTGGCGGAGAAGAATTTATCGGTGAGCGCCAGTAAATGCTGTTG‑CCATTGTTCCATGGAATAACGGCTTTGCAGCGTTTGGAACCAATCAAATAACGCTGCTAAAAATGCCGCCAATTGCCCCGCCAGCTGCCCTTTTAAGCCGAAACTGTTATCCAGCCCGAGGCTGTCTTGCCAAATGCCGTTTTCTTCacgc                                                                                                                                                                                   <  1:499477/225‑1 (MQ=255)                                                                                       atatAAAACAGGGTTTCATCGGTTTCCGCCGTGGCGGAGAAGAATTTATCGGTGAGCGCCAGTAAATGCTGTTG‑CCATTGTTCCATGGAATAACGGCTTTGCAGCGTTTGGAACCAATCAAATAACGCTGCTAAAAATGCCGCCAATTGCCCCGCCAGCTGCCCTTTTAAGCCGAAACTGTTATCCAGCCCGAGGCTGTCTTGCCAAATGCCGTTTTCTTCACGCAAGGCATAGCCCaacaac                                                                                                                                                                 <  1:451778/243‑1 (MQ=255)                                                                                       atatAAAACAGGGTTTCATCGGTTTCCGCCGTGGCGGAGAAGAATTTATCGGTGAGCGCCAGTAAATGCTGTTG‑CCATTGTTCCATGGAATAACGGCTTTGCAGCGTTTGGAACCAATCAAATAACGCTGCTAAAAATGCCGCCAATTGCCCCGCCAGCTGCCCTTTTAAGCCGAAACTGTTATCCAGCCCGAGGCTGTCTTGCCAAATGCCGTTTTCTTCACGCAAGGCATAGCCCAACAACATTCGCt                                                                                                                                                          >  1:496790/1‑250 (MQ=255)                                                                                       atatAAAACAGGGTTTCATCGGTTTCCGCCGTGGCGGAGAAGAATTTATCGGTGAGCGCCAGTAAATGCTGTTG‑CCATTGTTCCATGGAATAACGGCTTTGCAGCGTTTGGAACCAATCAAATAACGCTGCTAAAAATGCCGCCAATTGCCCCGCCAGCTGCCCTTTTAAGCCGAAACTGTTATCCAGCCCGAGGCTGTCTTGCCAAATGCCGTTTTCTTCACGCAAGGCATAGCCCAACAACATTCGCt                                                                                                                                                          >  1:368621/1‑250 (MQ=255)                                                                                            aaaCAGGGTTTCATCGGTTTCCGCCGTGGCGGAGAAGAATTTATCGGTGAGCGCCAGTAAATGCTGTTG‑CCATTGTTCCATGGAATAACGGCTTTGCAGCGTTTGGAACCAATCAAATAACGCTGCTAAAAATGCCGCCAATTGCCCCGCCAGCTGCCCttttaagc                                                                                                                                                                                                                                        <  1:374114/167‑6 (MQ=255)                                                                                             aaCAGGGTTTCATCGGTTTCCGCCGTGGCGGAGAAGAATTTATCGGTGAGCGCCAGTAAATGCTGTTG‑CCATTGTTCCATGGAATAACGGCTTTGCAGCGTTTGGAACCAATCAAATAACGCTGCTAAAAATGCCGCCAATTGCCCCGCCAGCTGCCCTTTTAAGCCGAAACTGTTATCCAGCCCGAGGCTGTCTTGCCAAATGCCGTTTTCTTCACGCAAGGCATAGCCCAACAACATTCGCTCCAAGcc                                                                                                                                                   <  1:593376/251‑1 (MQ=255)                                                                                             aaCAGGGTTTCATCGGTTTCCGCCGTGGCGGAGAAGAATTTATCGGTGAGCGCCAGTAAATGCTGTTG‑CCATTGTTCCATGGAATAACGGCTTTGCAGCGTTTGGAACCAATCAAATAACGCTGCTAAAAATGCCGCCAATTGCCCCGCCAGCTGCCCTTTTAAGCCGAAACTGTTATCCAGCCCGAGGCTGTCTTGCCAAATGCCGTTTTCTTCACGCAAGGCATAGCCCAACAACATTCGCTCCAAGcc                                                                                                                                                   <  1:566443/251‑1 (MQ=255)                                                                                                   gTTTCATCGGTTTCCGCCGTGGCGGAGAAGAATTTATCGGTGAGCGCCAGTAAATGCTGTTG‑CCATTGTTCCATGGAATAACGGCTTTGCAGCGTTTGGAGCCAATCAAATAACGCTGCTAAAAATGCCGCCAATTGCCCCGCCAGCTGCCCTTTTAAGCCGAAACTGTTATCCAGCCCGAGGCTGTCTTGCCAAATGCCGTTTTCTTCACGCAAGGCATagcc                                                                                                                                                                        <  1:664409/224‑4 (MQ=255)                                                                                                   gTTTCATCGGTTTCCGCCGTGGCGGAGAAGAATTTATCGGTGAGCGCCAGTAAATGCTGTTG‑CCATTGTTCCATGGAATAACGGCTTTGCAGCGTTTGGAACCAATCAAATAACGCTGCTAAAAATGCCGCCAATTGCCCCGCCAGCTGCCCTTTTAAGCCGAAACTGTTATCCAGCCCGAGGCTGTCTTGCCAAATGCCGTTTTCTTCACGCAAGGCATAGCCCAACAACATTCGCTCCAAGcccgc                                                                                                                                                >  1:625939/1‑245 (MQ=255)                                                                                                      tCATCGGTTTCCGCCGTGGCGGAGAAGAATTTATCGGTGAGCGCCAGTAAATGCTGTTG‑CCATTGTTCCATGGAATAACGGCTTTGCAGCGTTTGGAACCAATCAAATAACGCTGCTAAAAATGCCGCCAATTGCCCCGCCAGCTGCCCTTTTAAGCCGAAACTGTTATCCAGCCCGAGGCTGTCTTGCCAAATGCCGTTTTCTTCACGCAAGGCATAGCCCAACAACATTCGCTCCAAGCCCGCCTGCCa                                                                                                                                          >  1:323364/1‑251 (MQ=255)                                                                                                            gTTTCCGCCGTGGCGGAGAAGAATTTATCGGTGAGCGCCAGTAAATGCTGTTG‑CCATTGTTCCATGGAATAACGGCTTTGCAGCGTTTGGAACCAATCAAATAACGCTGCTAAAAATGCCGCCAATTGCCCCGCCAGCTGCCCTTTTAAGCCGAAACTGTTATCCAGCCCGAGGCTGTCTTGCCAAATGCCGTTTTCTTCACGCAAGGCATAGCCCAACAACATTCGCTCCAAGCCCGCCTGCCATGAAt                                                                                                                                     <  1:115079/250‑1 (MQ=255)                                                                                                                ccgccgTGGCGGAGAAGAATTTATCGGTGAGCGCCAGTAAATGCTGTTG‑CCATTGTTCCATGGAATAACGGCTTTGCAGCGTTTGGAACCAATCAAATAACGCTGCTAAAAATGCCGCCAATTGCCCCGCCAGCTGCCCTTTTAAGCCGAAACTGTTATCCAGCCCGAGGCTGTCTTGCCAAATGCCGTTTTCTTCACGCAAGGCATAGCCCAACAACATTCGCTCCAAGCCCGCCTGCCATGAATTGTAg                                                                                                                                <  1:646285/251‑1 (MQ=255)                                                                                                                   ccgtggcggagaagaaTTTATCGGTGAGCGCCAGTAAATGCTGTTG‑CCATTGTTCCATGGAATAACGGCTTTGCAGCGTTTGGAACCAATCAAATAACGCTGCTAAAAATGCCGCCAATTGCCCCGCCAGCTGCCCTTTTAAGCCGAAACTGTTATCCAGCCCGAGGCTGTCTTGCCAAATGCCGTTTTCTTCACGCAAGGCATAGCCCAACAACATTCGCTCCAAGCCCGCCTGCCATGAATTGTAGTTa                                                                                                                             <  1:654118/241‑1 (MQ=255)                                                                                                                                    ttATCGGTGAGCGCCAGTAAATGCTGTTG‑CCATTGTTCCATGGAATAACGGCTTTGCAGCGTTTGGAACCAATCAAATAACGCTGCTAAAAATGCCGCCAATTGCCCCGCCAGCTGCCCTTTTAAGCCGAAACTGTTATcc                                                                                                                                                                                                                          >  1:383515/1‑141 (MQ=255)                                                                                                                                     tATCGGTGAGCGCCAGTAAATGCTGTTG‑CCATTGTTCCATGGAATAACGGCTTTGCAGCGTTTGGAACCAATCAAATAACGCTGCTAAAAATGCCGCCAATTGCCCCGCCAGCTGCCCTTTTAAGCCGAAACTGTTATCCAGCCCGAGGCTGTCTTGCCAAATGCCGTTTTCTTCACGCAAGGCATAGCCCAACAACATTCGCTCCAAGCCCGCCTGCCATGAATTGTAGTTACTTTCCGCTTCGTCAttt                                                                                                           <  1:839993/251‑1 (MQ=255)                                                                                                                                      aTCGGTGAGCGCCAGTAAATGCTGTTG‑CCATTGTTCCATGGAATAACGGCTTTGCAGCGTTTGGAACCAATCAAATAACGCTGCTAAAAATGCCGCCAATTGCCCCGCCAGCTGCCCTTTTAAGCCGAAACTGTTATCCAGCCCGAGGCTGTCTTGCCAAATGCCGTTTTCTTCACGCAAGGCATAGCCCaacaa                                                                                                                                                                  >  1:548496/1‑192 (MQ=255)                                                                                                                                          gTGAGCGCCAGTAAATGCTGTTG‑CCATTGTTCCATGGAATAACGGCTTTGCAGCGTTTGGAACCAATCAAATAACGCTGCTAAAAATGCCGCCAATTGCCCCGCCAGCTGCCCTTTTAAGCCGAAACTGTTATCCAGCCCGAGGCTGTCTTGCCAAATGCCGTTTTCTTCACGCAAGGCATAGCCCAACAACATTCGCTCCAAGcc                                                                                                                                                   >  1:14466/1‑206 (MQ=255)                                                                                                                                                 ccAGTAAATGCTGTTG‑CCATTGTTCCATGGAATAACGGCTTTGCAGCGTTTGGAACCAATCAAATAACGCTGCTAAAAATGCCGCCAATTGCCCCGCCAGCTGCCCTTTTAAGCCGAAACTGTTATCCAGCCCGAGGCTGTCTTGCCAAATGCCGTTTTCTTCACGCAAGGCATAGCCCAACAACATTCGCTCCAAGCCCGCCTGCCATGAATTGTAGTTACTTTCCGCTTCGTCATTTTTTGCTAAGCCg                                                                                               >  1:190298/1‑251 (MQ=255)                                                                                                                                                   aGTAAATGCTGTTG‑CCATTGTTCCATGGAATAACGGCTTTGCAGCGTTTGGAACCAATCAAATAACGCTGCTAAAAATGCCGCCAATTGCCCCGCCAGCTGCCCTTTTAAGCCGAAACTGTTATCCAGCCCGAGGCTGTCTTGCCAAATGCCGTTTTCTTCACGCAAGGCATAGCCCAACAACATTCGCTCCAAGCCCGCCTGCCATGAATTGTAGTTACTTTCCGc                                                                                                                     <  1:691953/227‑1 (MQ=255)                                                                                                                                                                       gTTCCATGGAATAACGGCTTTGCAGCGTTTGGAACCAATCAAATAACGCTGCTAAAAATGCCGCCAATTGCGCCGCCAGCTGCCCTTTTAAGCCGAAACTGTTATCCAGCCCGAGGCTGTCTTGCCAAATGCCGTTTTCTTCACGCAAGGCATAGCCCAACAACATTCGCTCCAAGCCCGCCTGCCATGAATTGTAGTTACTTTCCGCTTCGTCATTTTTTGCTAAGCCGAAACGCACGCCGGCAttttt                                                                           >  1:439682/1‑250 (MQ=255)                                                                                                                                                                       gTTCCATGGAATAACGGCTTTGCAGCGTTTGGAACCAATCAAATAACGCTGCTAAAAATGCCGCCAATTGCCCCGCCAGCTGCCCTTTTAAGCCGAAACTGTTATCCAGCCCGAGGCTGTCTTGCCAAATGCCGTTTTCTTCACGCAAGGCATAGCCCAACAACATTCGCTCCAAGCCCGCCTGCCATGAATTGTAGTTACTTTCCGCTTCGTCATTTTTTGCTAAGCCGAAACGCACGCCGGCAttttt                                                                           >  1:380612/1‑250 (MQ=255)                                                                                                                                                                                                 gTTTGGAACCAATCAAATAACGCTGCTAAAAATGCCGCCAATTGCCCCGCCAGCTGCCCTTTTAAGCCGAAACTGTTATCCAGCCCGAGGCTGTCTTGCCAAATGCCGTTTTCTTCACGCAAGGCAt                                                                                                                                                                            >  1:672612/1‑127 (MQ=255)                                                                                                                                                                                                 gTTTGGAACCAATCAAATAACGCTGCTAAAAATGCCGCCAATTGCCCCGCCAGCTGCCCTTTTAAGCCGAAACTGTTATCCAGCCCGAGGCTGTCTTGCCAAATGCCGTTTTCTTCACGCAAGGCATAGCCCAACAACGTTCGCTCCAAGCCCGCCTGCCATGAATTGTAGTTACTTTCCGCTTCGTCATTTTTTGCTAAGCCGAAACGCACGCCGGCATTTTTCACCCAATCGCGAATTTGCGCCAAAt                                                 >  1:11493/1‑250 (MQ=255)                                                                                                                                                                                                 gTTTGGAACCAATCAAATAACGCTGCTAAAAATGCCGCCAATTGCCCCGCCAGCTGCCCTTTTAAGCCGAAACTGTTATCCAGCCCGAGGCTGTCTTGCCAAATGCCGTTTTCTTCACGCAAGGCATAGCCCAACAACATTCGCTCCAAGCCCGCCTGCCATGAATTGTAGTTACt                                                                                                                           <  1:246449/176‑1 (MQ=255)                                                                                                                                                                                                 gTTTGGAACCAATCAAATAACGCTGCTAAAAATGCCGCCAATTGCCCCGCCAGCTGCCCTTTTAAGCCGAAACTGTTATCCAGCCCGAGGCTGTCTTGCCAAATGCCGTTTTCTTCACGCAAGGCATAGCCCAACAACATTCGCTCCAAGCCCGCCTGCCATGAATTGTAGTTACTTTCCGCTTCGTCATTTTTTGCTAAGCCGAAACGCACGCCGGCATTTTTCACCCAATCGCGAATTTGCGCCAAAt                                                 >  1:294515/1‑250 (MQ=255)                                                                                                                                                                                                 gTTTGGAACCAATCAAATAACGCTGCTAAAAATGCCGCCAATTGCCC‑GCCAGCTGCCCTTTTAAGCCGAAACTGTTATCCAGCCCGAGGCTGTCTTGCCAAATGCCGTTTTCTTCACGCAAGGCATAGCCCAACAACATTCGCTCCAAGCCCGCCTGCCATGAATTGTAGTTACTTTCCGCTTCGTCATTTTTTGCTAAGCCGAAACGCACGCCGGCATTTTTCACCCAATCGCGAATTTGCGCCAAATc                                                >  1:132815/1‑250 (MQ=255)                                                                                                                                                                                                  tttGGAACCAATCAAATAACGCTGCTAAAAATGCCGCCAATTGCCCCGCCAGCTGCCCTTTTAAGCCGAAACTGTTATCCAGCCCGAGGCTGTCTTGCCAAATGCCGTTTTCTTCACGCAAGGCATAGCCCAACAACATTCGCTCCAAGCCCGCCTGCCATGAATTGTAGTTACTTTCCGCTTCGTCATTTTTTGCTAAGCCGAAACGCACGCCGGCATTTTTCACCCAATCGCGAATTTGCGCCAAATc                                                >  1:404536/1‑250 (MQ=255)                                                                                                                                                                                                  tttGGAACCAATCAAATAACGCTGCTAAAAATGCCGCCAATTGCCCCGCCAGCTGCCCTTTTAAGCCGAAACTGTTATCCAGCCCGAGGCTGTCTTGCCAAATGCCGTTTTCTTCACGCAAGGCATAGCCCAACAACATTCGCTCCAAGCCCGCCTGCCATGAATTGTAGTTACTTTCCGCTTCGTCATTTTTTGCTAAGCCGAAACGCACGCCGGCATTTTTCACCCAATCGCGAATTTGCGCCAAATc                                                <  1:814041/250‑1 (MQ=255)                                                                                                                                                                                                          caatcaaaTAACGCTGCTAAAAATGCCGCCAATTGCCCCGCCAGCTGCCCTTTTAAGCCGAAACTGTTATCCAGCCCGAGGCTGTCTTGCCAAATGCCGTTTTCTTCACGCAAGGCATAGCCCAACAACATTCGCTCCAAGCCCGCCTGCCATGAATTGTAGTTACTTTCCGCTTCGTCATTTTTTGCTAAGCCGAAACGCACGCCGGCATTTTTCACCCAATCGCGAATTTGCGCCAAATCCTGCAATTc                                       <  1:776139/245‑1 (MQ=255)                                                                                                                                                                                                              caaaTAACGCTGCTAAAAATGCCGCCAATTGCCCCGCCAGCTGCCCTTTTAAGCCGAAACTGTTATCCAGCCCGAGGCTGTCTTGCCAAATGCCGTTTTCTTCACGCAAGGCATAGCCCAACAACATTCGCTCCAAGCCCGCCTGCCATGAATTGTAGTTACTTTCCGCTTCGTCATTTTTTGCTAAGCCGAAACGCACGCCGGCATTTTTCACCCAATCGCGAATTTGCGCCAAATCCTGCAATTCAata                                   >  1:357684/3‑251 (MQ=255)                                                                                                                                                                                                               aaaTAACGCTGCTAAAAATGCCGCCAATTGCCCCGCCAGCTGCCCTTTTAAGCCGAAACTGTTATCCAGCCCGAGGCTGTCTTGCCAAATGCCGTTTTCTTCACGCAAGGCATAGCCCAACAACATTCGCTCCAAGCCCGCCTGCCATGAATTGTAGTTACTTTCCGCTTCGTCATTTTTTGCTAAGCCGAAACGCACGCCGGCATTTTTCACCCAATCGCGAATTTGCGCCAAATCCTGCAATTCAatat                                  <  1:407042/250‑1 (MQ=255)                                                                                                                                                                                                                    ccgctgcTAAAAATGCCGCCAATTGCCCCGCCAGCTGCCCTTTTAAGCCGAAACTGTTATCCAGCCCGAGGCTGTCTTGCCAAATGCCGTTTTCTTCACGCAAGGCATAGCCCAACAACATTCGCTCCAAGCCCGCCTGCCATGAATTGTAGTTACTTTCCGCTTCGTCATTTTTTGCTAAGCCGAAACGCACGCCGGCATTTTTCACCCAATCGCGAATTTGCGCCAAATCCTGCAATTCAATATTAAAg                             <  1:440149/246‑1 (MQ=255)                                                                                                                                                                                                                    aCGCTGCTAAAAATGCCGCCAATTGCCCCGCCAGCTGCCCTTTTAAGCCGAAACTGTTATCCAGCCCGAGGCTGTCTTGCCAAATGCCGTTTTCTTCACGCAAGGCATAGCCCAACAACATTCGCTCCAAGCCCGCCTGCCATGAATTGTAGTTACTTTCCGCTTCGTCATTTTTTGCTAAGCCGAAACGCACGCCGGCATTTTTCACCCAATCGCGAATTTGCGCCAAATCCTGCAATTCAATATTAAAg                             <  1:452350/251‑1 (MQ=255)                                                                                                                                                                                                                    aCGCTGCTAAAAATGCCGCCAATTGCCCCGCCAGCTGCCCTTTTAAGCCGAAACTGTTATCCAGCCCGAGGCTGTCTTGCCAAATGCCGTTTTCTTCACGCAAGGCATAGCCCAACAACATTCGCTCCAAGCCCGCCTGCCATGAATTGTAGTTACTTTCCGCTTCGTCATTTTTTGCTAAGCCGAAACGCACGCCGGCATTTTTCACCCAATCGCGAATTTGCGCCAAATCCTGCAATTCAATATTAAAg                             <  1:443594/251‑1 (MQ=255)                                                                                                                                                                                                                    aCGCTGCTAAAAATGCCGCCAATTGCCCCGCCAGCTGCCCTTTTAAGCCGAAACTGTTATCCAGCCCGAGGCTGTCTTGCCAAATGCCGTTTTCTTCACGCAAGGCATAGCCCAACAACATTCGCTCCAAGCCCGCCTGCCATGAATTGTAGTTACTTTCCGCTTCGTCATTTTTTGCTAAGCCGAAACGCACGCCGGCATTTTTCACCCAATCGCGAATTTGCGCCAAATCCTGCAATTCAATATTAAAg                             <  1:801952/251‑1 (MQ=255)                                                                                                                                                                                                                    aCGCTGCTAAAAATGCCGCCAATTGCCCCGCCAGCTGCCCTTTTAAGCCGAAACTGTTATCAAGCCCGAGGCTGTCTTGCCAAATGCCGTTTTCTTCACGCAAGGCATAGCCCAACAACATTCGCTCCAAGCCCGCCTGCCATGAATTGTAGTTACTTTCCGCTTCGTCATTTTTTGCTAAGCCGAAACGCACGCCGGCATTTTTCACCCAATCGCGAATTTGCGCCAAATCCTGCAATTCAATATTAAAg                             <  1:319988/251‑1 (MQ=255)                                                                                                                                                                                                                       ctgcTAAAAATGCCGCCAATTGCCCCGCCAGCTGCCCTTTTAAGCCGAAACTGTTATCCAGCCCGAGGCTGTCTTGCCAAATGCCGTTTTCTTCACGCAAGGCATAGCCCAACAACATTCGCTCCAAGCCCGCCTGCCATGAATTGTGGTTACTTTCCGCTTCGTCATTTTTTGCTAAGCCGAAACGCACGCCGGCATTTTTCACCCAATCGCGAATTTGCGCCAAATCCTGCAATTCAATATTAAAGCg                           <  1:246877/248‑1 (MQ=255)                                                                                                                                                                                                                         gcTAAAAATGCCGCCAATTGCCCCGCCAGCTGCCCTTTTAAGCCGAAACTGTTATCCAGCCCGAGGCTGTCTTGCCAAATGCCGTTTTCTTCACGCAAGGCATAGCCCAACAACATTCGCTCCAAGCCCGCCTGCCATGAATTGTAGTTACTTTCCGCTTCGTCATTTTTTGCTAGGCCGAAACGCACGCCGGCATTTTTCACCCAATCGCGAATTTGCGCCAAATCCTGCAATTCAATATTAAAGCGTTg                        >  1:113912/1‑251 (MQ=255)                                                                                                                                                                                                                         gcTAAAAATGCCGCCAATTGCCCCGCCAGCTGCCCTTTTAAGCCGAAACTGTTATCCAGCCCGAGGCTGTCTTGCCAAATGCCGTTTTCTTCACGCAAGGCATAGCCCAACAACATTCGCTCCAAGCCCGCCTGCCATGAATTGTAGTTACTTTCCGCTTCGTCATTTTTTGCTAAGCCGAAacg                                                                                          >  1:360994/1‑185 (MQ=255)                                                                                                                                                                                                                         gcTAAAAATGCCGCCAATTGCCCCGCCAGCTGCCCTTTTAAGCCGAAACTGTTATCCAGCCCGAGGCTGTCTTGCCAAATGCCGTTTTCTTCACGCAAGGCATAGCCCAACAACATTCGCTCCAAGCCCGCCTGCCATGAATTGTAGTTACTTTCCGCTTCGTCATTTTTTGCTAAGCCGAAACGCACGCCGGCATTTTTCACCCAATCGCGAATTTGCGCCAAATCCTGCAATTCAATATTAAAGCGTTg                        >  1:468304/1‑251 (MQ=255)                                                                                                                                                                                                                                               ccgCCAGCTGCCCTTTTAAGCCGAAACTGTTATCCAGCCCGAGGCTGTCTTGCCAAATGCCGTTTTCTTCACGCAAGGCATAGCCCAACAACATTCGCTCCAAGCCCGCCTGCCATGAATTGTAGTTACTTTCCGCTTCGTCATTTTTTGCTAAGCCGAAACGCACGCCGGCATTTTTCACCCAATCGCGAATTTGCGCCAAATCCTGCAATTCAATATTAAAGCGTTGGCGAATTGCCGGCACATCAAGc  <  1:56062/249‑1 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                    TGACATCCGCCACCACATCCACGCCCAACGCGTCGCTAAAATGCACCTCCGCTAACTGTTCGGCAAATTGTCGGATTACCTCTTTAATATAAAACAGGGTTTCATCGGTTTCCGCCGAGGAGGCGAAGAATTTATCGGTGAGCGCCAGTAAATGCTGTTG‑CCATTGTTCCACGGAATAACGGTTTTGCAGCGTTTGGAACCAGTCGAATAACGCCGCTAAAAATGCCGCCAATTGCCCTGCCAGTTGCCCTTTCAAACCGAAACTGTTATCCAGCCCGAGGCTATCCTGCCAAATGCCGTTTTCTTCGCGCAAGGCATAACCCAATAACATTCGCTCCAAGCCGGCTTGCCATGAATTGTAGTTACTTTCCGCCTCATCATTTTTTGCTAAGCCGAAACGCACGCCGGCATTTTTCACCCAATCACGAATTTGCGCCAAATCCTGCAATTCAATATTAAAGCGTTGGCGAATTGCCGGCACATCAAGC  >  1/1989716‑1990203

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑