| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | 1 | 1,987,235 | G→A | A26V (GCG→GTG) | unknown ← | 16S rRNA (guanine(1207)‑N(2))‑methyltransferase (EC 2.1.1.172) |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | 1 | 1,987,235 | 0 | G | A | 96.5% | 152.4 / NA | 57 | A26V (GCG→GTG) | unknown | 16S rRNA (guanine(1207)‑N(2))‑methyltransferase (EC 2.1.1.172) |
| Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (24/31); minor base C (0/2); total (24/33) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.04e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CCAGCAAAACCTCCTGCCCGGCGGTGCATTGTGAGAGAAGCTGCATGAGCTGGAAATGGCATTCCTGTTTATTCTTCGTCCAATAAAACAGGATCACATCGGCGCTGCCATGAAATTCAAGGGAAAAATCCACCGCACTTTGTGGCGTTTGATGTTGTCTGTTGGCATAGTCAAAATACCAGCTCCATGCGCGGGCGTTGAATCCCTGACGTTGTAAATCGGCGAGAAAATCATCGTTCGCCGCCCCGCTGAGCAGCAGGGACTTCCCGCTTAAAAAATCCAAATGCCGTTCCAACACTTCGCTTTCTGCACAAATCACGCTATTAATCCTATGGCTTATTGGTTAAAATGGGCGCCCATTATACTGGATTGAATTGATTTTAGATATGCAAAAAACGCGCCGACACACTTCCCGCCGAGATTTATTATTGCAGCAAATGGGCATCAGCCAATGGCAATTGG > 1/1986993‑1987454 | ccaGTAAAACTTCCTGTCCGGCGGTACATTGTGAGAGAAGCTGCATGAGCTGGAAATGGCATTCCTGTTTATTCTTCGTCCAATAAAACAGGATCACATCGGCGCTGCCATGAAATTCAAGGGAAAAATCCACCGCACTTTGTGGCGTTTGGTGTTGTCGGTTGGCGTAGTCAAAATACCAGCTCCATGCGCGGGCGTTGAATCCCTGGCGTTGTAAATCGGCGAGAAAATCATCATTCACCAcccc > 1:748261/1‑247 (MQ=255) cagtaaaaCTTCCTGTCCGGCGGTACATTGTGAGAGAAGCTGCATGAGCTGGAAATGGCATTCCTGTTTATTCTTCGTCCAATAAAACAGGATCACATCGGCGCTGCCATGAAATTCAAGGGAAAAATCCACCGCACTTTGTGGCGTTTGGTGTTGTCGGTTGGCGTAGTCAAAATACCAGCTCCATGCGCGGGCGTTGAATCCCTGGCGTTGTAAATCGGCGAGAAAATCATCATTCACCACCCCGGTGa > 1:70827/5‑251 (MQ=255) gtaaaaCTTCCTGTCCGGCGGTACATTGTGAGAGAAGCTGCATGAGCTGGAAATGGCATTCCTGTTTATTCTTCGTCCAATAAAACAGGATCACATCGGCGCTGCCATGAAATTCAAGGGAAAAATCCACCGCACTTTGTGGCGTTTGGTGTTGTCGGTTGGCGTAGTCAAAATACCAGCTCCATGCGCGGGCGTTGAATCCCTGGCGTTGTAAATCGGCGAGAAAATCATCATTCACCACCCCGGTGa > 1:69385/3‑249 (MQ=255) gcggTACATTGTGAGAGAAGCTGCATGAGCTGGAAATGGCATTCCTGTTTATTCTTCGTCCAATAAAACAGGATCACATCGGCGCTGCCATGAAATTCAAGGGAAAAATCCACCGCACTTTGTGGCGTTTGGTGTTGTCGGTTGGCGTAGTCAAAATACCAGCTCCATGCGCGGGCGTTGAATCCCTGGCGTTGTAAATCGGCGAGAAAATCATCATTCACCACCCCGGTGAGCAGCAGAGACTTCCCg > 1:374102/1‑249 (MQ=255) gcggTACATTGTGAGAGAAGCTGCATGAGCTGGAAATGGCATTCCTGTTTATTCTTCGTCCAATAAAACAGGATCACATCGGCGCTGCCATGAAATTCAAGGGAAAAATCCACCGCACTTTGTGGCGTTTGGTGTTGTCGGTTGGCGTAGTCAAAATACCAGCTCCATGCGCGGGCGTTGAATCCCTGGCGTTGTAAATCGGCGAGAAAATCATCATTCACCACCCCGGTGAGCAGCAGAGACTTCCCg > 1:621028/1‑249 (MQ=255) ttGTGAGAGAAGCTGCATGAGCTGGAAATGGCATTCCTGTTTATTCTTCGTCCAATAAAACAGGATCACATCGGCGCTGCCATGAAATTCAAGGGAAAAATCCACCGCACTTTGTGGCGTTTGGTGTTGTCGGTTGGCGTAGTCAAAATACCAGCTCCATGCGCGGGCGTTGAATCCCTGGCGTTGTAAATCGGCGAGAAAATCATCATTCACCACCCCGGTGa < 1:53771/224‑1 (MQ=255) gagagaAGCTGCATGAGCTGGAAATGGCATTCCTGTTTATTCTTCGTCCAATAAAACAGGATCACATCGGCGCTGCCATGAAATTCAAGGGAAAAATCCACCGCACTTTGTGGCGTTTGGTGTTGTCGGTTGGCGTAGTCAAAATACCAGCTCCATGCGCGGGCGTTGAATCCCTGGCGTTGTAAATCGGCGAGAAAATCATCATTCACCACCCCGGTGAGCAGCAGAGACTTCCCGCTTAAAAAATCCaa > 1:161844/1‑251 (MQ=255) gaAGCTGCATGAGCTGGAAATGGCATTCCTGTTTATTCTTCGTCCAATAAAACAGGATCACATCGGCGCTGCCATGAAATTCAAGGGAAAAATCCACCGCACTTTGTGGCGTTTGGTGTTGTCGGTTGGCGTAGTCAAAATACCAGCTCCATGCGCGGGCGTTGAATCCCTGGCGTTGTAAATCGGCGAGAAAATCATCATTCACCACCCCgg < 1:500441/213‑2 (MQ=255) tGCATGAGCTGGAAATGGCATTCCTGTTTATTCTTCGTCCAATAAAACAGGATCACATCGGCGCTGCCATGAAATTCAAGGGAAAAATCCACCGCACTTTGTGGCGTTTGGTGTTGTCGGTTGGCGTAGTCAAAATACCAGCTCCATGCGCGGGCGTTGAATCCCTGGCGTTGTAAATCGGCGAGAAAATCATCATTCACCACCCCGGTGAGCAGCAGAGACTTCCCGCTTAAAAAATCCAAATGCCGTTc < 1:111770/251‑1 (MQ=255) cTGGAAATGGCATTCCTGTTTATTCTTCGTCCAATAAAACAGGATCACATCGGCGCTGCCATGAAATTCAAGGGAAAAATCCACCGCACTTTGTGGCGTTTGGTGTTGTCGGTTGGCGTAGTCAAAATACCAGCTCCATGCGCGGGCGTTGAATCCCTGGCGTTGTAAATCGGCGAGAAAATCATCATTCACCACCCCGGTGAGCAGCAGAGACTTCCCGCTTAAAAAATCCAAATGCCGTTCCAacac < 1:696085/249‑1 (MQ=255) cTGGAAATGGCATTCCTGTTTATTCTTCGTCCAATAAAACAGGATCACATCGGCGCTGCCATGAAATTCAAGGGAAAAATCCACCGCACTTTGTGGCGTTTGGTGTTGTCGGTTGGCGTAGTCAAAATACCAGCTCCATGCGCGGGCGTTGAATCCCTGGCGTTGTAAATCGGCGAGAAAATCATCATTCACCACCCCGGTGAGCAGCAGAGACTTCCCGCTTAAAAAATCCAAATGCCGTTCCAACACtt < 1:833122/251‑1 (MQ=255) ggAAATGGCATTCCTGTTTATTCTTCGTCCAATAAAACAGGATCACATCGGCGCTGCCATGAAATTCAAGGGAAAAATCCACCGCACTTTGTGGCGTTTGGTGTTGTCGGTTGGCGTAGTCAAAATACCAGCTCCATGCGCGGGCGTTGAATCCCTGGCGTTGTAAATCGGCGAGAAAATCATCATTCACCACCCCGGTGAGCAGCAGAGACTTCCCGCTTAAAAAATCCAAATGCCGTTCCAACACTTCg > 1:227864/1‑251 (MQ=255) cTGTTTATTCTTCGTCCAATAAAACAGGATCACATCGGCGCTGCCATGAAATTCAAGGGAAAAATCCACCGCACTTTGTGGCGTTTGGTGTTGTCGGTTGGCGTAGTCAAAATACCAGCTCCATGCGCGGGCGTTGAATCCCTGGCGTTGTAAATCGGCGAGAAAATCATCATTCACCACCCCGGTGAGCAGCAGAGACTTCCCGCTTAAAAAATCCAAATGCCGTTCCAACACTTCGCTTTCTGCACaaa < 1:798096/251‑1 (MQ=255) tGTTTATTCTTCGTCCAATAAAACAGGATCACATCGGCGCTGCCATGAAATTCAAGGGAAAAATCCACCGCACTTTGTGGCGTTTGGTGTTGTCGGTTGGCGTAGTCAAAATACCAGCTCCATGCGCGGGCGTTGAATCCCTGGCGTTGTAAATCGGCGAGAAAATCATCATTCACCACCCCGGTGAGCAGCAGAGACTTCCCGCTTAAAAAATCCAAATGCCGTTCCAACACTTCGCTTTCTGCACAAAt > 1:22942/1‑251 (MQ=255) tGTTTATTCTTCGTCCAATAAAACAGGATCACATCGGCGCTGCCATGAAATTCAAGGGAAAAATCCACCGCACTTTGTGGCGTTTGGTGTTGTCGGTTGGCGTAGTCAAAATACCAGCTCCATGCGCGGGCGTTGAATCCCTGGCGTTGTAAATCGGCGAGAAAATCATCATTCACCACCCCGGTGAGCAGCAGAGACTTCCCGCTTAAAAAATCCAAATGCCGTTCCAACACTTCGCTTTCTGCACAAAt > 1:161682/1‑251 (MQ=255) gTTTATTCTTCGTCCAATAAAACAGGATCACATCGGCGCTGCCATGAAATTCAAGGGAAAAATCCACCGCACTTTGTGGCGTTTGGTGTTGTCGGTTGGCGTAGTCAAAATACCAGCTCCATGCGCGGGCGTTGAATCCCTGGCGTTGTAAATCGGCGAGAAAATCATCATTCACCACCCCGGTGAGcagcagag < 1:17806/195‑3 (MQ=255) tCCAATAAAACAGGATCACATCGGCGCTGCCATGAAATTCAAGGGAAAAATCCACCGCACTTTGTGGCGTTTGGTGTTGTCGGTTGGCGTAGTCAAAATACCAGCTCCATGCGCGGGCGTTGAATCCCTGGCGTTGTAAATCGGCGAGAAAATCATCATTCACCACCCCGGTGAGCAGCAGAGACTTCCCGCTTAAAAAATCCAAATGCCGTTCCAACACTTCGCTTTCTGCACAAATCACGCTATTAATc < 1:657975/251‑1 (MQ=255) tCCAATAAAACAGGATCACATCGGCGCTGCCATGAAATTCAAGGGAAAAATCCACCGCACTTTGTGGCGTTTGGTGTTGTCGGTTGGCGTAGTCAAAATACCAGCTCCATGCGCGGGCGTTGAATCCCTGGCGTTGTAAATCGGCGAGAAAATCATCATTCACCACCCCGGTGAGCAGCAGAGACTTCCCGCTTAAAAAATCCAAATGCCGTTCCAACACTTCGCTTTCTGCACAAATCACGCTATTAATc < 1:288961/251‑1 (MQ=255) tCCAATAAAACAGGATCACATCGGCGCTGCCATGAAATTCAAGGGAAAAATCCACCGCACTTTGTGGCGTTTGGTGTTGTCGGTTGGCGTAGTCAAAATACCAGCTCCATGCGCGGGCGTTGAATCCCTGGCGTTGTAAATCGGCGAGAAAATCATCATTCACCACCCCGGTGAGCAGCAGAGACTTCCCGCTTAAAAAATCCAAATGCCGTTCCAACACTTCGCTTTCTGCACAAATCACGCTATTAATc < 1:380476/251‑1 (MQ=255) cAATAAAACAGGATCACATCGGCGCTGCCATGAAATTCAAGGGAAAAATCCACCGCACTTTGTGGCGTTTGGTGTTGTCGGTTGGCGTAGTCAAAATACCAGCTCCATGCGCGGGCGTTGAATCCCTGGCGTTGTAAATCGGCGAGAAAATCATCATTCACCACCCCGGTGAGCAGCAGAGACTTCCCGCTTAAAAAATCCAAATGCCGTTCCAACACTTCGCTTTCTGCACAAATCACGCTATTAATCCt < 1:284892/251‑1 (MQ=255) cAATAAAACAGGATCACATCGGCGCTGCCATGAAATTCAAGGGAAAAATCCACCGCACTTTGTGGCGTTTGGTGTTGTCGGTTGGCGTAGTCAAAATACCAGCTCCATGCGCGGGCGTTGAATCCCTGGCGTTGTAAATCGGCGAGAAAATCATCATTCACCACCCCGGTGAGCAGCAGAGACTTCCCGCTTAAAAAATCCAAATGCCGTTCCAACACTTCGCTTTCTGCACAAATCACGCTATTAATCCt < 1:117471/251‑1 (MQ=255) aaaaCAGGATCACATCGGCGCTGCCATGAAATTCAAGGGAAAAATCCACCGCACTTTGTGGCGTTTGGTGTTGTCGGTTGGCGTAGTCAAAATACCAGCTCCATGCGCGGGCGTTGAATCCCTGGCGTTGTAAATCGGCGAGAAAATCATCATTCACCACCCCGGTGAGCAGCAGAGACTTCCCGCTTAAAAAATCCAAATGc < 1:790000/203‑1 (MQ=255) aaaaCAGGATCACATCGGCGCTGCCATGAAATTCAAGGGAAAAATCCACCGCACTTTGTGGCGTTTGGTGTTGTCGGTTGGCGTAGTCAAAATACCAGCTCCATGCGCGGGCGTTGAATCCCTGGCGTTGTAAATCGGCGAGAAAATCATCATTCACCACCCCGGTGAGCAGCAGAGACTTCCCGCTTAAAAAATCCAAATGCCGTTCCAACACTTCGCTTTCTGCACAAATCACGCTATTAATCCTATgg < 1:842067/251‑1 (MQ=255) aaaCAGGATCACATCGGCGCTGCCATGAAATTCAAGGGAAAAATCCACCGCACTTTGTGGCGTTTGGTGTTGTCGGTTGGCGTAGTCAAAATACCAGCTCCATGCGCGGGCGTTGAATCCCTGGCGTTGTAAATCGGCGAGAAAATCATCATTCACCACCCCGGTGAGCAGCAGAGACTTCCCGCTTAAAAAATCCAAATGCCGTTCCAACACTTCGCTTTCTGCACAAATCACGCTATTAATCCTATGGc < 1:268589/251‑1 (MQ=255) aaCAGGATCACATCGGCGCTGCCATGAAATTCAAGGGAAAAATCCACCGCACTTTGTGGCGTTTGGTGTTGTCGGTTGGCGTAGTCAAAATACCAGCTCCATGCGCGGGCGTTGAATCCCTGGCGTTGTAAATCGGCGAGAAAATCATCATTCACCACCCCGGTGAGCAGCAGAGACTTCCCGCTTAAAAAATCCAAATGCCGTTCCAACACTTCGCTTTCTGCACAAATCACGCTATTAATCCTATGGCt < 1:282305/251‑1 (MQ=255) gATCACATCGGCGCTGCCATGAAATTCAAGGGAAAAATCCACCGCACTTTGTGGCGTTTGGTGTTGTCGGTTGGCGTAGTCAAAATACCAGCTCCATGCGCGGGCGTTGAATCCCTGGCGTTGTAAATCGGCGAGAAAATCATCATTCACCACCCCGGTGAGCAGCAGAGAc < 1:753793/172‑1 (MQ=255) aTCACATCGGCGCTGCCATGAAATTCAAGGGAAAAATCCACCGCACTTTGTGGCGTTTGGTGTTGTCGGTTGGCGTAGTCAAAATACCAGCTCCATGCGCGGGCGTTGAATCCCTGGCGTTGTAAATCGGCGAGAAAATCATCATTCACCACCCCgg > 1:102828/1‑156 (MQ=255) gCCATGAAATTCAAGGGAAAAATCCACCGCACTTTGTGGCGTTTGGTGTTGTCGGTTGGCGTAGTCAAAATACCAGCTCCATGCGCGGGCGTTGAATCCCTGGCGTTGTAAATCGGCGAGAAAATCATCATTCACCACCCCGGTGAGCAGCAGAGACTTCCCGCTTAAACAATCCAAATGCCGTTCCAACACTTCGCTTTCTGCACAAATCACGCTATTAATCCTATGGc > 1:442945/1‑230 (MQ=255) aTGAAATTCAAGGGAAAAATCCACCGCACTTTGTGGCGTTTGGTGTTGTCGGTTGGCGTAGTCAAAATACCAGCTCCATGCGCGGGCGTTGAATCCCTGGCGTTGTAAATCGGCGAGAAAATCATCATTCACCACCCCGGTGAGCAGCAGAGACTTCCCGCTTAAAAAATCCAAATGCCGTTCCAACACTTCGCTTTCTGCACAAATCACGCTATTAATCCTATGGCTTATTGGTTAAAATGGGCGCCCAt < 1:205206/251‑1 (MQ=255) aaaTTCAAGGGAAAAATCCACCGCACTTTGTGGCGTTTGGTGTTGTCGGTTGGCGTAGTCAAAATACCAGCTCCATGCGCGGGCGTTGAATCCCTGGCGTTGTAAATCGGCGAGAAAATCATCATTCACCACCCCGGTGAGCAGCAGAGACTTCCCGCTTAAAAAATCCAAATGCCGTTCCAACACTTCGCTTTCTGCACAAATCACGCTATTAATCCTATGGCTTATTGGTTAAAATGGGCGCCCATtat < 1:325650/251‑1 (MQ=255) aTTCAAGGGAAAAATCCACCGCACTTTGTGGCGTTTGGTGTTGTCGGTTGGCGTAGTCAAAATACCAGCTCCATGCGCGGGCGTTGAATCCCTGGCGTTGTAAATCGGCGAGAAAATCATCATTCACCACCCCGGTGAGCAGCAGAGACTTCCCGCTTAAAAAATCCAAATGCCGTTCCAACACTTCGCTTTCTGCACAAATCACGCTATTAATCCTATGGCTTATTGGTTAAAATGGGCGCCCATTATAc < 1:560422/251‑1 (MQ=255) aTTCAAGGGAAAAATCCACCGCACTTTGTGGCGTTTGGTGTTGTCGGTTGGCGTAGTCAAAATACCAGCTCCATGCGCGGGCGTTGAATCCCTGGCGTTGTAAATCGGCGAGAAAATCATCATTCACCACCCCGGTGAGCAGCAGAGACTTCCCGCTTAAAAAATCCAAATGCCGTTCCAACACTTCGCTTTCTGCACAAATCACGCTATTAATCCTATGGCTTATTGGTTAAAATGGGCGCCCATTATAc < 1:594207/251‑1 (MQ=255) ccACCGCACTTTGTGGCGTTTGGTGTTGTCGGTTGGCGTAGTCAAAATACCAGCTCCATGCGCGGGCGTTGAATCCCTGGCGTTGTAAATCGGCGAGAAAATCATCATTCACCACCCCGGTGAGCAGCAGAGACTTCCCGCTTAAAAAATCCAAATGCCGTTCCAACACTTCGCTTTCTGCACaaa > 1:481550/1‑186 (MQ=255) cACTTTGTGGCGTTTGGTGTTGTCGGTTGGCGTAGTCAAAATACCAGCTCCATGCGCGGGCGTTGAATCCCTGGCGTTGTAAATCGGCGAGAAAATCATCATTCACCACCCCGGTGAGCAGCAGAGACTTCCCGCTTAAAAAATCCAAATGCCGTTCCAac > 1:462963/1‑161 (MQ=255) cACTTTGTGGCGTTTGGTGTTGTCGGTTGGCGTAGTCAAAATACCAGCTCCATGCGCGGGCGTTGAATCCCTGGCGTTGTAAATCGGCGAGAAAATCATCATTCACCACCCCGGTGAGCAGCAGAGACTTCCCGCTTAAAAAATCCAAATGCCGTTCCAACACTTCGCTTTCTGCACAAATCACGCTATTAATCCt < 1:687501/196‑1 (MQ=255) cTTTGTGGCGTTTGGTGTTGTCGGTTGGCGTAGTCAAAATACCAGCTCCATGCGCGGGCGTTGAATCCCTGGCGTTGTAAATCGGCGAGAAAATCATCATTCACCACCCCGGTGAGCAGCAGAGACTTCCCGCTTAAAAAATCCAAATGc < 1:742007/150‑1 (MQ=255) cTTTGTGGCGTTTGGTGTTGTCGGTTGGCGTAGTCAAAATACCAGCTCCATGCGCGGGCGTTGAATCCCTGGCGTTGTAAATCGGCGAGAAAATCATCATTCACCACCCCGGTGAGCAGCAGAGACTTCCCGCTTAAAAAATCCAAATGCCGTTCCAACACTTCGCTTTCTGCACAAATCACGCTATTAATCCTATGGc > 1:382188/1‑199 (MQ=255) ttGTGGCGTTTGGTGTTGTCGGTTGGCGTAGTCAAAATACCAGCTCCATGCGCGGGCGTTGAATCCCTGGCGTTGTAAATCGGCGAGAAAATCATCATTCACCACCCCGGTGAGCAGCAGAGACTTCCCGCTTAAAAAATCCAAATGCCGTTCCAACACTTCGCTTTCTGCACAAATCACGCTATTAATc < 1:393692/190‑1 (MQ=255) gtgGCGTTTGGTGTTGTCGGTTGGCGTAGTCAAAATACCAGCTCCATGCGCGGGCGTTGAATCCCTGGCGTTGTAAATCGGCGAGAAAATCATCATTCACCACCCCGGTGAGCAGCAGAGACTTCCCGCTTAAAAAATCCAAATGCCGTTCCAACACTTCGCTTTCTGCACAAATCACGCTATTAATCCTATGGCTTATTGGTTAAAATGGGCGCCCATTATACTg > 1:549478/1‑226 (MQ=255) gTTTGGTGTTGTCGGTTGGCGTAGTCAAAATACCAGCTCCATGCGCGGGCGTTGAATCCCTGGCGTTGTAAATCGGCGAGAAAATCATCATTCACCACCCCGGTGAGCAGCAGAGACTTCCCGCTTAAAAAATCCAAATGCCGTTCCAACACTTCGCTTTCTGCACAAATCACGCTATTAATCCTATGGCTTATTGGTTAAAATGGGCGCCCATTATAc > 1:656655/1‑219 (MQ=255) tttGGTGTTGTCGGTTGGCGTAGTCAAAATACCAGCTCCATGCGCGGGCGTTGAATCCCTGGCGTTGTAAATCGGCGAGAAAATCATCATTCACCACCCCGGTGAGCAGCAGAGACTTCCCGCTTAAAAAATCCAAATGCCGTTCCAACACTTCGCTTTCTGCACAAATCACGCTATTAATCCTATGGCTTATTGGTTAAAATGGGCGCCCATTATACTGGATTGAATTGATTTTAGATATGCAAGAAAc < 1:673120/250‑1 (MQ=255) tttGGTGTTGTCGGTTGGCGTAGTCAAAATACCAGCTCCATGCGCGGGCGTTGAATCCCTGGCGTTGTAAATCGGCGAGAAAATCATCATTCACCACCCCGGTGAGCAGCAGAGACTTCCCGCTTAAAAAATCCAAATGCCGTTCCAACACTTCGCTTTCTGCACAAATCACGCTATTAATCCTATGGCTTATTGGTTAAAATGGGCGCCCATTATACTGGATTGAATTGATTTTAGATATGCAAGAAAc < 1:503310/250‑1 (MQ=255) cggTTGGCGTAGTCAAAATACCAGCTCCATGCGCGGGCGTTGAATCCCTGGCGTTGTAAATCGGCGAGAAAATCATCATTCACCACCCCGGTGAGCAGCAGAGACTTCCCGCTTAAAAAATCCAAATGCCGTTCCAACACTTCGCTTTCTGCACAAATCACGCTATTAATCCTATGGCTTATTgg > 1:739912/3‑185 (MQ=255) cggTTGGCGTAGTCAAAATACCAGCTCCATGCGCGGGCGTTGAATCCCTGGCGTTGTAAATCGGCGAGAAAATCATCATTCACCACCCCGGTGAGCAGCAGAGACTTCCCGCTTAAAAAATCCAAATGCCGTTCCAACACTTCGCTTTCTGCACAAATCACGCTATTAATCCTATGGCTTATTGGTTAAAATGGGCGCCCATTATACTGGATTGAATTGATTTTAGATATGCAAGAAACGCGCCGacacac < 1:564288/249‑1 (MQ=255) ggcgtAGTCAAAATACCAGCTCCATGCGCGGGCGTTGAATCCCTGGCGTTGTAAATCGGCGAGAAAATCATCATTCACCACCCCGGTGAGCAGCAGAGACTTCCCGCTTAAAAAATCCAAATGCCGTTCCAACACTTCGCTTTCTGCACAAATCACGCTATTAATCCTATGGCTTATTGGTTAAAATGGGCGCCCATTATACTGGATTGAATTGATTTTAGATATGCAAGAAACGCGCCGACACACTTcc > 1:10092/5‑250 (MQ=255) gTCAAAATACCAGCTCCATGCGCGGGCGTTGAATCCCTGGCGTTGTAAATCGGCGAGAAAATCATCATTCACCACCCCGGTGAGCAGCAGAGACTTCCCGCTTAAAAAATCCAAATGCCGTTCCAACACTTCGCTTTCTGCACAAATCACGCTATTAATCCTATGGCTTATTGGTTAAAATGGGCGCCCATTATACTGGATTGAATTGAtt > 1:598067/1‑211 (MQ=255) cAAAATACCAGCTCCATGCGCGGGCGTTGAATCCCTGGCGTTGTAAATCGGCGAGAAAATCATCATTCACCACCCCGGTGAGCAGCAGAGACTTCCCGCTTAAAAAATCCAAATGCCGTTCCAACACTTCGCTTTCTGCACAAATCACGCTATTAATcc < 1:753925/159‑1 (MQ=255) gCTCCATGCGCGGGCGTTGAATCCCTGGCGTTGTAAATCGGCGAGAAAATCATCATTCACCACCCCGGTGAGCAGCAGAGACTTCCCGCTTAAAAAATCCAAATGCCGTTCCAACACTTCGCTTTCTGCACAAATCACGCTATTAATc > 1:666166/1‑148 (MQ=255) cTCCATGCGCGGGCGTTGAATCCCTGGCGTTGTAAATCGGCGAGAAAATCATCATTCACCACCCCGGTGAGCAGCAGAGACTTCCCGCTTAAAAAATCCAAATGCCGTTCCAACACTTCGCTTTCTGCACAAATCACGCTATTAATc > 1:717476/1‑147 (MQ=255) gcgcGGGCGTTGAATCCCTGGCGTTGTAAATCGGCGAGAAAATCATCATTCACCACCCCGGTGAGCAGCAGAGACTTCCCGCTTAAAAAATCCAAATGCCGTTCCAACACTTCGCTTTCTGCACAAATCACGCTATTAATCCTATGGCTTATTGGTTAAAATGGGCGCCCATTATAc < 1:583273/177‑1 (MQ=255) ggCGTTGAATCCCTGGCGTTGTAAATCGGCGAGAAAATCATCATTCACCACCCCGGTGAGCAGCAGAGACTTCCCGCTTAAAAAATCCAAATGCCGTTCCAACACTTCGCTTTCTGCACAAATCACGCTATTAATCCTATGGCTTATTGGTTAAAATGGGCGCCCATTATACTGGATTGAATTGATTTTAGATATGCAAGAAACGCGCCGACACACTTCCCGCCGAGATTTATTATTGCAGCAAATGGGCa > 1:450767/1‑251 (MQ=255) gAATCCCTGGCGTTGTAAATCGGCGAGAAAATCATCATTCACCACCCCGGTGAGCAGCAGAGACTTCCCGCTTAAAAAATCCAAATGCCGTTCCAACACTTCGCTTTCTGCACAAATCACGCTATTAATCCTATGGCTTATTGGTTAAAATGGGCGCCCATTATACTGGATTGAATTGATTTTAGATATGCAAGAAACGCGCCGACACACTTCCCGCCGAGATTTATTATTGCAGCAAATGGGCATCAGc > 1:536005/1‑250 (MQ=255) aTCCCTGGCGTTGTAAATCGGCGAGAAAATCATCATTCACCACCCCGGTGAGCAGCAGAGACTTCCCGCTTAAAAAATCCAAATGCCGTTCCAACACTTCGCTTTCTGCACAAATCACGCTATTAATCCTATGGCTTATTGGTTAAAATGGGCGCCCATTATACTGGATTGAATTGATTTTAGATATGCAAGAAACGCGCCGACACACTTCCCGCCGAGATTTATTATTGCAGCAAATGGGCATCAGCCaa > 1:185177/1‑251 (MQ=255) cccTGGCGTTGTAAATCGGCGAGAAAATCATCATTCACCACCCCGGTGAGCAGCAGAGACTTCCCGCTTAAAAAATCCAAATGCCGTTCCAACACTTCGCTTTCTGCACAAATCACGCTATTAATCCTATGGCTTATTGGTTAAAATGGGCGCCCATTATACTGGATTGAATTGATTTTAGata < 1:133275/184‑1 (MQ=255) ctggcGTTGTAAATCGGCGAGAAAATCATCATTCACCCCCCCGGTGAGCAGCAGAGACTTCCCGCTTAAAAAATCCAAATGCCGTTCCAACACTTCGCTTTCTGCACAAATCACGCTATTAATCCTATGGCTTATTGGTTAAAATGGGCGCCCATTATACTGGATTGAATTGATTTTAGATATGCAAGAAACGCGCCGACACACTTCCCGCCGAGATTTATTATTGCAGCAAATGGGCATCAGCCAATGGc < 1:262236/247‑1 (MQ=255) ctggcGTTGTAAATCGGCGAGAAAATCATCATTCACCCCCCCGGTGAGCAGCAGAGACTTCCCGCTTAAAAAATCCAAATGCCGTTCCAACACTTCGCTTTCTGCACAAATCACGCTATTAATCCTATGGCTTATTGGTTAAAATGGGCGCCCATTATACTGGATTGAATTGATTTTAGATATGCAAGAAACGCGCCGACACACTTCCCGCCGAGATTTATTATTGCAGCAAATGGGCATCAGCCAATGGc < 1:81522/247‑1 (MQ=255) ctGTAAATCGGCGAGAAAATCATCATTCACCACCCCGGTGAGCAGCAGAGACTTCCCGCTTAAAAAATCCAAATGCCGTTCCAACACTTCGCTTTCTGCACAAATCACGCTATTAATCCTATGGCTTATTGGTTAAAATGGGCGCCCATTATACTGGATTGAATTGATTTTAGATATGCAAGAAACGCGCCGACACACTTCCCGCCGAGATTTATTATTGCAGCAAATGGGCATCAGCCAATGGCAATTgg < 1:894461/250‑1 (MQ=255) | CCAGCAAAACCTCCTGCCCGGCGGTGCATTGTGAGAGAAGCTGCATGAGCTGGAAATGGCATTCCTGTTTATTCTTCGTCCAATAAAACAGGATCACATCGGCGCTGCCATGAAATTCAAGGGAAAAATCCACCGCACTTTGTGGCGTTTGATGTTGTCTGTTGGCATAGTCAAAATACCAGCTCCATGCGCGGGCGTTGAATCCCTGACGTTGTAAATCGGCGAGAAAATCATCGTTCGCCGCCCCGCTGAGCAGCAGGGACTTCCCGCTTAAAAAATCCAAATGCCGTTCCAACACTTCGCTTTCTGCACAAATCACGCTATTAATCCTATGGCTTATTGGTTAAAATGGGCGCCCATTATACTGGATTGAATTGATTTTAGATATGCAAAAAACGCGCCGACACACTTCCCGCCGAGATTTATTATTGCAGCAAATGGGCATCAGCCAATGGCAATTGG > 1/1986993‑1987454 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |