Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,986,973	G→A	G113G (GGC→GGT)	unknown ←	16S rRNA (guanine(1207)‑N(2))‑methyltransferase (EC 2.1.1.172)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,986,973	0	G	A	100.0%	161.8 / NA	55	G113G (GGC→GGT)	unknown	16S rRNA (guanine(1207)‑N(2))‑methyltransferase (EC 2.1.1.172)
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (33/22);  total (33/22)

GTTTTTTAGCGTAGAAAGCAATAATGCCGTGCCGCCATCCAGTTCGGCGGCGCTGAATACGCCGGGCAACGCATAGATAGTTAAATCGGCAATTTGTGGCGATTGATAGGATTTCCAGAAAGTTTTCAGTTCAAAGTGCGGTGGATTTTTTAACTGAAAATGATACAGACTGCAACGGCGGGCGGAGTCGATTTTGTGAATATCGCCGTAATCGGCAAGCATTTTTTCGGCGGAACGCACGCCGCTACGATTTTCGCCCACCAGCAAAACCTCCTGCCCGGCGGTGCATTGTGAGAGAAGCTGCATGAGCTGGAAATGGCATTCCTGTTTATTCTTCGTCCAATAAAACAGGATCACATCGGCGCTGCCATGAAATTCAAGGGAAAAATCCACCGCACTTTGTGGCGTTTGATGTTGTCTGTTGGCATAGTCAAAATACCAGCTCCATGCGCGGGCGTTGAATCCCTGAC  >  1/1986733‑1987202                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                      gTTTTTTAGCGTAGAAAGCAATAATGCCGTGCCGCCATCCAGCTCGGCGGCGCTGAATACGCCGGGCAACGCATAGATAGTTAAATCGGCAAGTTGCGACGATTGATAGGATTTCCAGAAAGTTTTCAGTTCAAAGTGCGGTGGATTTTTTAATTGAAAATGATACAGACTGCAACGACGGGCGGAGTCGATTTTGTGAATATCGCCGTAATCGGCAAGCATTTTTTCGGCGGAACGCACACCACTACGa                                                                                                                                                                                                                              >  1:645805/1‑250 (MQ=255)  ttttttAGCGTAGAAAGCAATAATGCCGTGCCGCCATCCAGCTCGGCGGCGCTGAATACGCCGGGCAACGCATAGATAGTTAAATCGGCAAGTTGCGACGATTGATAGGATTTCCAGAAAGTTTTCAGTTCAAAGTGCGGTGGATTTTTTAATTGAAAATGATACAGACTGCAACGACGGGCGGAGTCGATTTTGTGAATATCGCCGTAATCGGCAAGCATTTTTTCGGCGGAACGCACACCACTACGAtt                                                                                                                                                                                                                            <  1:7695/251‑1 (MQ=255)                   cAATAATGCCGTGCCGCCATCCAGCTCGGCGGCGCTGAATACGCCGGGCAACGCATAGTTAGTTAAATCGGCAAGTTGCGACGATTGATAGGATTTCCAGAAAGTTTTCAGTTCAAAGTGCGGTGGATTTTTTAATTGAAAATGATACAGACTGCAACGACGGGCGGAGTCGATTTTGTGAATATCGCCGTAATCGGCAAGCATTTTTTCGGCGGAACGCACACCACTACGATTTTCGCCCACCAGTaaaa                                                                                                                                                                                                           >  1:327340/1‑251 (MQ=255)                   cAATAATGCCGTGCCGCCATCCAGCTCGGCGGCGCTGAATACGCCGGGCAACGCATAGATAGTTAAATCGGCAAGTTGCGACGATTGATAGGATTTCCAGAAAGTTTTCAGTTCAAAGTGCGGTGGATTTTTTAATTGAAAATGATACAGACTGCAACGACGGGCGGAGTCGATTTTGTGAATATCGCCGTAATCGGCAAGCATTTTTTCGGCGGAACGCACACCACTACGATTTTCGCCCACCAGTaaaa                                                                                                                                                                                                           >  1:267767/1‑251 (MQ=255)                                ccgccATCCAGCTCGGCGGCGCTGAATACGCCGGGCAACGCATAGATAGTTAAATCGGCAAGTTGCGACGATTGATAGGATTTCCAGAAAGTTTTCAGTTCAAAGTGCGGTGGATTTTTTAATTGAAAATGATACAGACTGCAACGACGGGCGGAGTCGATTTTGTGAATATCGCCGTAATCGGCAAGCATTTTTTCGGCGGAACGCACACCACTACGATTTTCGCCCACCAGTAAAACTTCCTGTCcgg                                                                                                                                                                                               >  1:726689/1‑250 (MQ=255)                                     aTCCAGCTCGGCGGCGCTGAATACGCCGGGCAACGCATAGATAGTTAAATCGGCAAGTTGCGACGATTGATAGGATTTCCAGAAAGTTTTCAGTTCAAAGTGCGGTGGATTTTTTAATTGAAAATGATACAGACTGCAACGACGGGCGGAGTCGATTTTGTGAATATCGCCGTAATCGGCAAGCATTTTTTCGGCGGAACGCACACCACTACGa                                                                                                                                                                                                                              >  1:366719/1‑214 (MQ=255)                                     aTCCAGCTCGGCGGCGCTGAATACGCCGGGCAACGCATAGATAGTTAAATCGGCAAGTTGCGACGATTGATAGGATTTCCAGAAAGTTTTCAGTTCAAAGTGCGGTGGATTTTTTAATTGAAAATGATACAGACTGCAACGACGGGCGGAGTCGATTTTGTGAATATCCCCGTAATCGGCAAGCATTTTTTCGGCGGAACGCACACCACTACGATTTTCGCCCACCAGTAAAACTTCCTGTCCGGCGGta                                                                                                                                                                                          >  1:768600/1‑249 (MQ=255)                                       ccAGCTCGGCGGCGCTGAATACGCCGGGCAACGCATAGATAGTTAAATCGGCAAGTTGCGACGATTGATAGGATTTCCAGAAAGTTTTCAGTTCAAAGTGCGGTGGATTTTTTAATTGAAAATGATACAGACTGCAACGACGGGCGGAGTCGATTTTGTGAATATCGCCGTAATCGGCAAGCATTTTTTCGGCGGAACGCACACCACTACGATTTTCGCCCACCAGTAAAACTTCCTGTCCGGCGGTACAt                                                                                                                                                                                       <  1:452063/251‑1 (MQ=255)                                       ccAGCTCGGCGGCGCTGAATACGCCGGGCAACGCATAGATAGTTAAATCGGCAAGTTGCGACGATTGATAGGATTTCCAGAAAGTTTTCAGTTCAAAGTGCGGTGGATTTTTTAATTGAAAATGATACAGACTGCAACGACGGGCGGAGTCGATTTTGTGAATATCGCCGTAATCGGCAAGCATTTTTTCGGCGGAACGCACACCACTACGATTTTCGCCCACCAGTAAAACTTCCTGTCCGGCGGTACAt                                                                                                                                                                                       <  1:385221/251‑1 (MQ=255)                                       ccAGCTCGGCGGCGCTGAATACGCCGGGCAACGCATAGATAGTTAAATCGGCAAGTTGCGACGATTGATAGGATTTCCAGAAAGTTTTCAGTTCAAAGTGCGGTGGATTTTTTAATTGAAAATGATACAGACTGCAACGACGGGCGGAGTCGATTTTGTGAATATCGCCGTAATCGGCAAGCATTTTTTCGGCGGAACGCACACCACTACGATTTTCGCCCACCAGTAAAACTTCCTGTCCGGCGGTACAt                                                                                                                                                                                       <  1:899841/251‑1 (MQ=255)                                                     cTGAATACGCCGGGCAACGCATAGATAGTTAAATCGGCAAGTTGCGACGATTGATAGGATTTCCAGAAAGTTTTCAGTTCAAAGTGCGGTGGATTTTTTAATTGAAAATGATACAGACTGCAACGACGGGCGGAGTCGATTTTGTGAATATCGCCGTAATCGGCAAGCATTTTTTCGGCGGAACGCACACCACTACGATTTTCGCCCACCAGTAAAACTTCCTGTCCGGCGGTACATTGTGAGAGAAg                                                                                                                                                                            >  1:150425/1‑248 (MQ=255)                                                       gAATACGCCGGGCAACGCATAGATAGTTAAATCGGCAAGTTGCGACGATTGATAGGATTTCCAGAAAGTTTTCAGTTCAAAGTGCGGTGGATTTTTTAATTGAAAATGATACAGACTGCAACGACGGGCGGAGTCGATTTTGTGAATATCGCCGTAATCGGCAAGCATTTTTTCGGCGGAACGCACACCACTACGATTTTCGCCCACCAGTAAAACTTCCTGTCCGGCGGTACAt                                                                                                                                                                                       <  1:599727/235‑1 (MQ=255)                                                       gAATACGCCGGGCAACGCATAGATAGTTAAATCGGCAAGTTGCGACGATTGATAGGATTTCCAGAAAGTTTTCAGTTCAAAGTGCGGTGGATTTTTTAATTGAAAATGATACAGACTGCAACGACGGGCGGAGTCGATTTTGTGAATATCGCCGTAATCGGCAAGCATTTTTTCGGCGGAACGCACACCACTACGATTTTCGCCCACCAGTAAAACTTCCTGTCCGGCGGTACATTGTGAGAGAAGCTGCa                                                                                                                                                                       >  1:699636/1‑251 (MQ=255)                                                       gAATACGCCGGGCAACGCATAGATAGTTAAATCGGCAAGTTGCGACGATTGATAGGATTTCCAGAAAGTTTTCAGTTCAAAGTGCGGTGGATTTTTTAATTGAAAATGATACAGACTGCAACGACGGGCGGAGTCGATTTTGTGAATATCGCCGTAATCGGCAAGCATTTTTTCGGCGGAACGCACACCACTACGATTTTCGCCCACCAGTAAAACTTCCTGTCCGGCGGTACATTGTGAGAGAAGCTGCa                                                                                                                                                                       >  1:110471/1‑251 (MQ=255)                                                       gAATACGCCGGGCAACGCATAGATAGTTAAATCGGCAAGTTGCGACGATTGATAGGATTTCCAGAAAGTTTTCAGTTCAAAGTGCGGTGGATTTTTTAATTGAAAATGATACAGACTGCAACGACGGGCGGAGTCGATTTTGTGAATATCGCCGTAATCGGCAAGCATTTTTTCGGCGGAACGCACACCACTACGATTTTCGCCCACCAGTAAAACTTCCTGTCCGGCGGTACATTGTGAGAGAAGCTGCa                                                                                                                                                                       >  1:194344/1‑251 (MQ=255)                                                                  gCAACGCATAGATAGTTAAATCGGCAAGTTGCGACGATTGATAGGATTTCCAGAAAGTTTTCAGTTCAAAGTGCGGTGGATTTTTTAATTGAAAATGATACAGACTGCAACGACGGGCGGAGTCGATTTTGTGAATATCGCCGTAATCGGCAAGCATTTTTTCGGCGGAACGCACACCACTACGATTTTCGCCCACCAGTAAAACTTCCTGTCCGGCGGTACATTGTgag                                                                                                                                                                                 <  1:275620/230‑1 (MQ=255)                                                                     aCGCATAGATAGTTAAATCGGCAAGTTGCGACGATTGATAGGATTTCCAGAAAGTTTTCAGTTCAAAGTGCGGTGGATTTTTTAATTGAAAATGATACAGACTGCAACGACGGGCGGAGTCGATTTTGTGAATATCGCCGTAATCGGCAAGCATTTTTTCGGCGGAACGCACACCACTACGATTTTCGCCCACCAGTAAAACTTCCTGTCCGGCGGTACATTGTGAGAGAAGCTGCATGAGCTGGAAATgg                                                                                                                                                         >  1:99561/1‑251 (MQ=255)                                                                      cGCATAGATAGTTAAATCGGCAAGTTGCGACGATTGATAGGATTTCCAGAAAGTTTTCAGTTCAAAGTGCGGTGGATTTTTTAATTGAAAATGATACAGACTGCAACGACGGGCGGAGTCGATTTTGTGAATATCGCCGTAATCGGCAAGCATTTTTTCGGCGGAACGCACACCACTACGa                                                                                                                                                                                                                              >  1:352346/1‑181 (MQ=255)                                                                      cGCATAGATAGTTAAATCGGCAAGTTGCGACGATTGATAGGATTTCCAGAAAGTTTTCAGTTCAAAGTGCGGTGGATTTTTTAATTGAAAATGATACAGACTGCAACGACGGGCGGAGTCGATTTTGTGAATATCGCCGTAATCGGCAAGCATTTTTTCGGCGGAACGCACACCACTACGATTTTCGCCCACCAGTAAAACTTCCTGTCCGGCGGTACATTGTGAGAGAAGCTGCATGAGCTGGAAATgg                                                                                                                                                         >  1:845888/1‑250 (MQ=255)                                                                      cGCATAGATAGTTAAATCGGCAAGTTGCGACGATTGATAGGATTTCCAGAAAGTTTTCAGTTCAAAGTGCGGTGGATTTTTTAATTGAAAATGATACAGACTGCAACGACGGGCGGAGTCGATTTTGTGAATATCGCCGTAATCGGCAAGCATTTTTTCGGCGGAACGCACACCACTACGATTTTCGCCCACCAGTAAAACTTCCTGTCCGGCGGTACATTGTGAGAGAAGCTGCATGAGCTGGAAATgg                                                                                                                                                         >  1:749738/1‑250 (MQ=255)                                                                      cGCATAGATAGTTAAATCGGCAAGTTGCGACGATTGATAGGATTTCCAGAAAGTTTTCAGTTCAAAGTGCGGTGGATTTTTTAATTGAAAATGATACAGACTGCAACGACGGGCGGAGTCGATTTTGTGAATATCGCCGTAATCGGCAAGCATTTTTTCGGCGGAACGCACACCACTACGATTTTCGCCCACCAGTAAAACTTCCTGTCCGGCGGTACATTGTGAGAGAAGCTGCATGAGCTGGAAATgg                                                                                                                                                         >  1:212149/1‑250 (MQ=255)                                                                       gCATAGATAGTTAAATCGGCAAGTTGCGACGATTGATAGGATTTCCAGAAAGTTTTCAGTTCAAAGTGCGGTGGATTTTTTAATTGAAAATGATACAGACTGCAACGACGGGCGGAGTCGATTTTGTGAATATCGCCGTAATCGGCAAGCATTTTTTCGGCGGAACGCACACCACTACGATTTTCGCCCACCAGTAAAACTTCCTGTCCGGCGGTACATTGTGAGAGAAGCTGCATGAGCTGGAAATGGCa                                                                                                                                                       >  1:169108/1‑251 (MQ=255)                                                                              tagTTAAATCGGCAAGTTGCGACGATTGATAGGATTTCCAGAAAGTTTTCAGTTCAAAGTGCGGTGGATTTTTTAATTGAAAATGATACAGACTGCAACGACGGGCGGAGTCGATTTTGTGAATATCGCCGTAATCGGCAAGCATTTTTTCGGCGGAACGCACACCACTACGATTTTCGCCCACCAGTAAAACTTCCTGTCCGGCGGTACATTGTGAGAGAAGCTGCATGAGCTGGAAATGGCATTc                                                                                                                                                    >  1:529807/1‑247 (MQ=255)                                                                              tagTTAAATCGGCAAGTTGCGACGATTGATAGGATTTCCAGAAAGTTTTCAGTTCAAAGTGCGGTGGATTTTTTAATTGAAAATGATACAGACTGCAACGACGGGCGGAGTCGATTTTGTGAATATCGCCGTAATCGGCAAGCATTTTTTCGGCGGAACGCACACCACTACGATTTTCGCCCACCAGTAAAACTTCCTGTCCGGCGGTACATTGTGAGAGAAGCTGCATGAGCTGGAAATGGCATTc                                                                                                                                                    >  1:783633/1‑247 (MQ=255)                                                                                    aaTCGGCAAGTTGCGACGATTGATAGGATTTCCAGAAAGTTTTCAGTTCAAAGTGCGGTGGATTTTTTAATTGAAAATGATACAGACTGCAACGACGGGCGGAGTCGATTTTGTGAATATCGCCGTAATCGGCAAGCATTTTTTCGGCGGAACGCACACCACTACGATTTTCGCCCACCAGTAAAACTTCCTGTCCGGCGGTACATTGTGAGAGAAGCTGCATGAGCTGGAAATGGCATTCCTGTTTAttc                                                                                                                                          <  1:244789/251‑1 (MQ=255)                                                                                                     gATTGATAGGATTTCCAGAAAGTTTTCAGTTCAAAGTGCGGTGGATTTTTTAATTGAAAATGATACAGACTGCAACGACGGGCGGAGTCGATTTTGTGAATATCGCCGTAATCGGCAAGCATTTTTTCGGCGGAACGCACACCACTACGATTTTCGCCCACCAGTAAAACTTCCTGTCCGGCGGTACAt                                                                                                                                                                                       >  1:34984/1‑189 (MQ=255)                                                                                                     gATTGATAGGATTTCCAGAAAGTTTTCAGTTCAAAGTGCGGTGGATTTTTTAATTGAAAATGATACAGACTGCAACGACGGGCGGAGTCGATTTTGTGAATATCGCCGTAATCGGCAAGCATTTTTTCGGCGGAACGCACACCACTACGATTTTCGCCCACCAGTAAAACTTCCTGTCCGGCGGTACATTGTGAGAGAAGCTGCATGAGCTGGAAATGGCATTCCTGTTTATTCTTCGTCCAATAAAACAg                                                                                                                         >  1:381789/1‑251 (MQ=255)                                                                                                           tAGGATTTCCAGAAAGTTTTCAGTTCAAAGTGCGGTGGATTTTTTAATTGAAAATGATACAGACTGCAACGACGGGCGGAGTCGATTTTGTGAATATCGCCGTAATCGGCAAGCATTTTTTCGGCGGAACGCACACCACTACGATTTTCGCCCACCAGTAAAACTTCCTGTCCGGCGGTACATTGTGAGAGAAGCTGCATGAGCTGGAAATGGCATTCCTGTTTATTCTTCGTCCAATAAAACAGGATcac                                                                                                                   <  1:193855/251‑1 (MQ=255)                                                                                                               aTTTCCAGAAAGTTTTCAGTTCAAAGTGCGGTGGATTTTTTAATTGAAAATGATACAGACTGCAACGACGGGCGGAGTCGATTTTGTGAATATCGCCGTAATCGGCAAGCATTTTTTCGGCGGAACGCACACCACTACGATTTTCGCCCACCAGTAAAACTTCCTGTCCGGCGGTACATTGTGAGAGAAGCTGCATGAGCTGGAAATGGCATTCCTGTTTATTCTTCGTCCAATAAAACAGGATCACATc                                                                                                                <  1:6723/250‑1 (MQ=255)                                                                                                               aTTTCCAGAAAGTTTTCAGTTCAAAGTGCGGTGGATTTTTTAATTGAAAATGATACAGACTGCAACGACGGGCGGAGTCGATTTTGTGAATATCGCCGTAATCGGCAAGCATTTTTTCGGCGGAACGCACACCACTACGATTTTCGCCCACCAGTAAAACTTCCTGTCCGGCGGTACATTGTGAGAGAAGCTGCATGAGCTGGAAATGGCATTCCTGTTTATTCTTCGTCCAATAAAACAGGATCACATCg                                                                                                               <  1:688114/251‑1 (MQ=255)                                                                                                                  tCCAGAAAGTTTTCAGTTCAAAGTGCGGTGGATTTTTTAATTGAAAATGATACAGACTGCAACGACGGGCGGAGTCGATTTTGTGAATATCGCCGTAATCGGCAAGCATTTTTTCGGCGGAACGCACACCACTACGATTTTCGCCCACCAGTAAAACTTCCTGTCCGGCGGTACATTGTGAGAGAAGCTGCATGAGCTGGAAATGGCATTCCTGTTTATTCTTCGTCCAATAAAACAGGATCACATCGgcg                                                                                                            >  1:55890/1‑251 (MQ=255)                                                                                                                  tCCAGAAAGTTTTCAGTTCAAAGTGCGGTGGATTTTTTAATTGAAAATGATACAGACTGCAACGACGGGCGGAGTCGATTTTGTGAATATCGCCGTAATCGGCAAGCATTTTTTCGGCGGAACGCACACCACTACGATTTTCGCCCACCAGTAAAACTTCCTGTCCGGCGGTACATTGTGAGAGAAGCTGCATGAGCTGGAAATGGCATTCCTGTTTATTCTTCGTCCAATAAAACAGGATCACATCGgcg                                                                                                            <  1:794608/251‑1 (MQ=255)                                                                                                                             ttCAGTTCAAAGTGCGGTGGATTTTTTAATTGAAAATGATACAGACTGCAACGACGGGCGGAGTCGATTTTGTGAATATCGCCGTAATCGGCAAGCATTTTTTCGGCGGAACGCACACCACTACGATTTTCGCCCACCAGTAAAACTTCCTGTCCGGCGGTACATTGTGAGAGAAGCTGCATGAGCTGGAAATGGCATTCCTGTTTATTCTTCGTCCAATAAAACAGGATCACATCGGCGCTGCCATGaaa                                                                                                 <  1:821885/251‑1 (MQ=255)                                                                                                                                                            gAAAATGATACAGACTGCAACGACGGGCGGAGTCGATTTTGTGAATATCGCCGTAATCGGCAAGCATTTTTTCGGCGGAACGCACACCACTACGATTTTCGCCCACCAGTAAAACTTCCTGTCCGGCGGTACATTGTGAGAGAAGCTGCATGAGCTGGAAATGGCATTCCTGTTTATTCTTCGTCCAATAAAACAGGATCACATCGGCGCTGCCATGAAATTCAAGGGAAAAATCCACCGCACTTTGTgg                                                                   >  1:53459/1‑250 (MQ=255)                                                                                                                                                              aaaTGATACAGACTGCAACGACGGGCGGAGTCGATTTTGTGAATATCGCCGTAATCGGCAAGCATTTTTTCGGCGGAACGCACACCACTACGATTTTCGCCCACCAGTAAAACTTCCTGTCCGGCGGTACATTGTGAGAGAAGCTGCATGAGCTGGaaa                                                                                                                                                            >  1:772392/1‑159 (MQ=255)                                                                                                                                                                aTGATACAGACTGCAACGACGGGCGGAGTCGATTTTGTGAATATCGCCGTAATCGGCAAGCATTTTTTCGGCGGAACGCACACCACTACGATTTTCGCCCACCAGTAAAACTTCCTGTCCGGCGGTACAt                                                                                                                                                                                       >  1:392336/1‑130 (MQ=255)                                                                                                                                                                aTGATACAGACTGCAACGACGGGCGGAGTCGATTTTGTGAATATCGCCGTAATCGGCAAGCATTTTTTCGGCGGAACGCACACCACTACGATTTTCGCCCACCAGTAAAACTTCCTGTCCGGCGGTACATTGTgagag                                                                                                                                                                               >  1:105046/1‑138 (MQ=255)                                                                                                                                                                aTGATACAGACTGCAACGACGGGCGGAGTCGATTTTGTGAATATCGCCGTAATCGGCAAGCATTTTTTCGGCGGAACGCACACCACTACGATTTTCGCCCACCAGTAAAACTTCCTGTCCGGCGGTACATTGTGAGAGAAGCTGCATGAGCTGGAAATGGCATTCCTGTTTATTCTTCGTCCAATAAAACAGGATCACATCGGCGCTGCCATGAAATTCAAggg                                                                                         >  1:105341/1‑224 (MQ=255)                                                                                                                                                                aTGATACAGACTGCAACGACGGGCGGAGTCGATTTTGTGAATATCGCCGTAATCGGCAAGCATTTTTTCGGCGGAACGCACACCACTACGATTTTCGCCCACCAGTAAAACTTCCTGTCCGGCGGTACATTGTGAGAGAAGCTGCATGAGCTGGAAATGGCATTCCTGTTTATTCTTCGTCCAATAAAACAGGATCACATCGGCGCTGCCATGAAATTCAAGGGAAAAAt                                                                                   <  1:520717/230‑1 (MQ=255)                                                                                                                                                                  gATACAGACTGCAACGACGGGCGGAGTCGATTTTGTGAATATCGCCGTAATCGGCAAGCATTTTTTCGGCGGAACGCACACCACTACGATTTTCGCCCACCAGTAAAACTTCCTGTCCGGCGGTACATTGTGAGAGAAGCTGCATGAGCTGGAAATGGCATTCCTGTTTAttc                                                                                                                                          >  1:311964/1‑173 (MQ=255)                                                                                                                                                                   aTACAGACTGCAACGACGGGCGGAGTCGATTTTGTGAATATCGCCGTAATCGGCAAGCATTTTTTCGGCGGAACGCACACCACTACGATTTTCGCCCACCAGTAAAACTTCCTGTCCGGCGGTACATTGTGAGAGAAGCTGCATGAGCTGGAAATGGCATTCCTGTTTATTCTTCGTCCAATAAAACAGGATCACATCGGCGCTGCCATGAAATTCAAGGGAAAAATCCACCGCACTTTGTGGCGTTTggt                                                           >  1:414259/1‑249 (MQ=255)                                                                                                                                                                          cTGCAACGACGGGCGGAGTCGATTTTGTGAATATCGCCGTAATCGGCAAGCATTTTTTCGGCGGAACGCACACCACTACGATTTTCGCCCACCAGTAAAACTTCCTGTCCGGCGGTACATTGTGAGAGAAGCTGCATGAGCTGGAAATGGCATTCCTGTTTATTCTTCGTCCAATAAAACAGGATcaca                                                                                                                  <  1:807440/189‑1 (MQ=255)                                                                                                                                                                          cTGCAACGACGGGCGGAGTCGATTTTGTGAATATCGCCGTAATCGGCAAGCATTTTTTCGGCGGAACGCACACCACTACGATTTTCGCCCACCAGTAAAACTTCCTGTCCGGCGGTACATTGTGAGAGAAGCTGCATGAGCTGGAAATGGCATTCCTGTTTATTCTTCGTCCAATAAAACAGGATCACATCGGCGCTGCCATGAAATTCAAGGGAAAAATCCACCGCACttt                                                                       >  1:233494/1‑232 (MQ=255)                                                                                                                                                                          cTGCAACGACGGGCGGAGTCGATTTTGTGAATATCGCCGTAATCGGCAAGCATTTTTTCGGCGGAACGCACACCACTACGATTTTCGCCCACCAGTAAAACTTCCTGTCCGGCGGTACATTGTGAGAGAAGCTGCATGAGCTGGAAATGGCATTCCTGTTTATTCTTCGTCCAATAAAACAGGATCACATCGGCGCTGCCATGAAATTCAAGGGAAAAATCCACCGCACTTTGTGGCGTTTggtg                                                          <  1:741319/245‑4 (MQ=255)                                                                                                                                                                            gCAACGACGGGCGGAGTCGATTTTGTGAATATCGCCGTAATCGGCAAGCATTTTTTCGGCGGAACGCACACCACTACGATTTTCGCCCACCAGTAAAACTTCCTGTCCGGCGGTACATTGTGAGAGAAGCTGCATGAGCTGGAAATGGCATTCCTGTTTATTCTTCGTCCAATAAAACAGGATCACATCGGCGCTGCCATGAAATTCAAGGGAAAAATCCACCGCACTTTGTGGCGTTTGGTGTTGTcggt                                                  >  1:369840/1‑248 (MQ=255)                                                                                                                                                                                       cggAGTCGATTTTGTGAATATCGCCGTAATCGGCAAGCATTTTTTCGGCGGAACGCACACCACTACGATTTTCGCCCACCAGTAAAACTTCCTGTCCGGCGGTACATTGTGAGAGAAGCTGCATGAGCTGGAAATGGCATTCCTGTTTATTCTTCGTCCAATAAAACAGGATCACATCGGCGCTGCCATGAAATTCAAGGGAAAAATCCACCGCACTTTGTGGCGTTTGGTGTTGTCGGTTGGCGTAGTc                                        <  1:381376/250‑1 (MQ=255)                                                                                                                                                                                             cGATTTTGTGAATATCGCCGTAATCGGCAAGCATTTTTTCGGCGGAACGCACACCACTACGATTTTCGCCCACCAGTAAAACTTCCTGTCCGGCGGTACATTGTGAGAGAAGCTGCATGAGCTGGAAATGGCATTCCTGTTTATTCTTCGTCCAATAAAACAGGATCACATCGGCGCTGCCATGAAATTCAAGGGAAAAATCCACCGCACTTTGTGGCGTTTGGTGTTGTCGGTTGGCGTAGTCAAAATa                                  >  1:871367/1‑250 (MQ=255)                                                                                                                                                                                             cGATTTTGTGAATATCGCCGTAATCGGCAAGCATTTTTTCGGCGGAACGCACACCACTACGATTTTCGCCCACCAGTAAAACTTCCTGTCCGGCGGTACATTGTGAGAGAAGCTGCATGAGCTGGAAATGGCATTCCTGTTTATTCTTCGTCCAATAAAACAGGATCACATCGGCGCTGCCATGAAATTCAAGGGAAAAATCCACCGCACTTTGTGGCGTTTGGTGTTGTCGGTTGGCGTAGTCAAAATAc                                 <  1:786551/251‑1 (MQ=255)                                                                                                                                                                                                 tttGTGAATATCGCCGTAATCGGCAAGCATTTTTTCGGCGGAACGCACACCACTACGATTTTCGCCCACCAGTAAAACTTCCTGTCCGGCGGTACATTGTGAGAGAAGCTGCATGAGCTGGAAATGGCATTCCTGTTTATTCTTCGTCCAATAAAACAGGATCACATCGgcgc                                                                                                           <  1:539352/173‑1 (MQ=255)                                                                                                                                                                                                         tatCGCCGTAATCGGCAAGCATTTTTTCGGCGGAACGCACACCACTACGATTTTCGCCCACCAGTAAAACTTCCTGTCCGGCGGTACATTGTGAGAGAAGCTGCATGAGCTGGAAATGGCATTCCTGTTTATTCTTCGTCCAATAAAACAGGATCACATCGGCGCTGCCATGAAATTCAAGGGAAAAATCCACCGCACTTTGTGGCGTTTGGTGTTGTCGGTTGGCGTAGTCAAAATACCAGCTCCATgcg                     <  1:220115/251‑1 (MQ=255)                                                                                                                                                                                                             gCCGTAATCGGCAAGCATTTTTTCGGCGGAACGCACACCACTACGATTTTCGCCCACCAGTAAAACTTCCTGTCCGGCGGTACATTGTGAGAGAAGCTGCATGAGCTGGAAATGGCATTCCTGTTTATTCTTCGTCCAATAAAACAGGATCACATCGgcgc                                                                                                           >  1:804373/1‑161 (MQ=255)                                                                                                                                                                                                              ccGTAATCGGCAAGCATTTTTTCGGCGGAACGCACACCACTACGATTTTCGCCCACCAGTAAAACTTCCTGTCCGGCGGTACATTGTGAGAGAAGCTGCATGAGCTGGAAATGGCATTCCTGTTTATTCTTCGTCCAATAAAACAGGATCACATCGgcgc                                                                                                           <  1:294721/160‑1 (MQ=255)                                                                                                                                                                                                                gTAATCGGCAAGCATTTTTTCGGCGGAACGCACACCACTACGATTTTCGCCCACCAGTAAAACTTCCTGTCCGGCGGTACATTGTGAGAGAAGCTGCATGAGCTGGAAATGGCATTCCTGTTTATTCTTCGTCCAATAAAACAGGATCACATCGGCGCTGCCATGAAATTCAAGGGAAAAATCCACCGCACTTTGTGGCGTTTGGTGTTGTCGGTTGGCGTAGTCAAAATACCAGCTCCATGCGCGGGCGt              >  1:272452/1‑251 (MQ=255)                                                                                                                                                                                                                            cATTTTTTCGGCGGAACGCACACCACTACGATTTTCGCCCACCAGTAAAACTTCCTGTCCGGCGGTACATTGTGAGAGAAGCTGCATGAGCTGGAAATGGCATTCCTGTTTATTCTTCGTCCAATAAAACAGGATCACATCGGCGCTGCCATGAAATTCAAGGGAAAAATCCACCGCACTTTGTGGCGTTTGGTGTTGTCGGTTGGCGTAGTCAAAATACCAGCTCCATGCGCGGGCGTTGAATCCCTggc  <  1:516195/251‑3 (MQ=255)                                                                                                                                                                                                                                         gAACGCACACCACTACGATTTTCGCCCACCAGTAAAACTTCCTGTCCGGCGGTACATTGTGAGAGAAGCTGCATGAGCTGGAAATGGCATTCCTGTTTATTCTTCGTCCAATAAAACAGGATCACATCGGCGCTGCCATGAAATTCAAGGGAAAAATCCACCGCACTTTGTGGCGTTTGGTGTTGTCGGTTGGCGTAGTCAAAATACCAGCTc                           <  1:688054/213‑1 (MQ=255)                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                      GTTTTTTAGCGTAGAAAGCAATAATGCCGTGCCGCCATCCAGTTCGGCGGCGCTGAATACGCCGGGCAACGCATAGATAGTTAAATCGGCAATTTGTGGCGATTGATAGGATTTCCAGAAAGTTTTCAGTTCAAAGTGCGGTGGATTTTTTAACTGAAAATGATACAGACTGCAACGGCGGGCGGAGTCGATTTTGTGAATATCGCCGTAATCGGCAAGCATTTTTTCGGCGGAACGCACGCCGCTACGATTTTCGCCCACCAGCAAAACCTCCTGCCCGGCGGTGCATTGTGAGAGAAGCTGCATGAGCTGGAAATGGCATTCCTGTTTATTCTTCGTCCAATAAAACAGGATCACATCGGCGCTGCCATGAAATTCAAGGGAAAAATCCACCGCACTTTGTGGCGTTTGATGTTGTCTGTTGGCATAGTCAAAATACCAGCTCCATGCGCGGGCGTTGAATCCCTGAC  >  1/1986733‑1987202

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑