Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	314,751	C→T	E410K (GAA→AAA)	unknown ←	Mobile element protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	314,751	0	C	T	100.0%	232.0 / NA	80	E410K (GAA→AAA)	unknown	Mobile element protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (43/37);  total (43/37)

TCGTAGTAACGTAAACCATCAATCGTTACGCCATTATGCTGAATAGTTCGTTTTTCGCTTGGTAAGAAATCGAGTAATAACGTTTGTTCATCAACAGGCATTTGCGGATAACCACAACCGACATGATCTTTATCACCAAAAATCCCCAATCGCCATTTTTGAACAGGCGACATACCTAGCGCAGAGTGGGTGCGTTTGTGATACACGTTTACAATATAGTTAATCAGCCACGTTTCAAATTCATCTAAGGTCATTACCGCTTCTGCTTCCGATTGATAGGTATCTTTCTCTTTCGTATTTGAGAATGTTGTACCACTCAAACCGTGAACTTCTTTCATAAACGTACCGATTACACGCTCAATGTGTCCGCCAAATTCAGGACGACCAACAGGGCGGAACTCAAGGGCAATACCGTGTGCCTCACAAGATTTGGATAAATCCAATGAACGGAAGTCTGCTCCATTATCCACGTGGATTTTATT  >  1/314510‑314991                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                 tCGTAGTAACGTAAACCATCAATCGTTACGCCATTATGCTGAATAGTTCGTTTTTCGCTTGGTAAGAAATCGAGTAATAACGTTTGTTCATCAACAGGCATTTGCGGATAACCACAACCGACATGATCTTTATCACCAAAAATCCCCAATCGCCATTTTTGAACAGGCGACATACCTAGCGCAGAGTGGGTGCGTTTGTGATACACGTTTACAATATAGTTAATCAGCCACGTTTCAAATTTATCTAAGGt                                                                                                                                                                                                                                         <  1:121289/251‑1 (MQ=255) tCGTAGTAACGTAAACCATCAATCGTTACGCCATTATGCTGAATAGTTCGTTTTTCGCTTGGTAAGAAATCGAGTAATAACGTTTGTTCATCAACAGGCATTTGCGGATAACCACAACCGACATGATCTTTATCACCAAAAATCCCCAATCGCCATTTTTGAACAGGCGACATACCTAGCGCAGAGTGGGTGCGTTTGTGATACACGTTTACAATATAGTTAATCAGCCACGTTTCAAATTTATCTAAGGt                                                                                                                                                                                                                                         >  1:42090/1‑251 (MQ=255) tCGTAGTAACGTAAACCATCAATCGTTACGCCATTATGCTGAATAGTTCGTTTTTCGCTTGGTAAGAAATCGAGTAATAACGTTTGTTCATCAACAGGCATTTGCGGATAACCACAACCGACATGATCTTTATCACCAAAAATCCCCAATCGCCATTTTTGAACAGGCGACATACCTAGCGCAGAGTGGGTGCGTTTGTGATACACGTTTACAATATAGTTAATCAGCCACGTTTCAAATTTATCTAAGGt                                                                                                                                                                                                                                         >  1:697151/1‑251 (MQ=255)       taACGTAAACCATCAATCGTTACGCCATTATGCTGAATAGTTCGTTTTTCGCTTGGTAAGAAATCGAGTAATAACGTTTGTTCATCAACAGGCATTTGCGGATAACCACAACCGACATGATCTTTATCACCAAAAATCCCCAATCGCCATTTTTGAACAGGCGACATACCTAGCGCAGAGTGGGTGCGTTTGTGATACACGTTTACAATATAGTTAATCAGCCACGTTTCAAATTTATCTAAGGTCATTAc                                                                                                                                                                                                                                   <  1:195971/251‑1 (MQ=255)        aaCGTAAACCATCAATCGTTACGCCATTATGCTGAATAGTTCGTTTTTCGCTTGGTAAGAAATCGAGTAATAACGTTTGTTCATCAACAGGCATTTGCGGATAACCACAACCGACATGATCTTTATCACCAAAAATCCCCAATCGCCATTTTTGAACAGGCGACATACCTAGCGCAGAGTGGGTGCGTTTGTGATACACGTTTACAATATAGTTAATCAGCCACGTTTCAAATTTATCTAAGGTCATTAc                                                                                                                                                                                                                                   >  1:384648/1‑250 (MQ=255)                ccATCAATCGTTACGCCATTATGCTGAATAGTTCGTTTTTCGCTTGGTAAGAAATCGAGTAATAACGTTTGTTCATCAACAGGCATTTGCGGATAACCACAACCGACATGATCTTTATCACCAAAAATCCCCAATCGCCATTTTTGAACAGGCGACATACCTAGCGCAGAGTGGGTGCGTTTGTGATACACGTTTACAATATAGTTAATCAGCCACGTTTCAAATTTATCTAAGGTCATTACCGCTTCTg                                                                                                                                                                                                                           >  1:724526/1‑250 (MQ=255)                               ccATTATGCTGAATAGTTCGTTTTTCGCTTGGTAAGAAATCGAGTAATAACGTTTGTTCATCAACAGGCATTTGCGGATAACCACAACCGACATGATCTTTATCACCAAAAATCCCCAATCGCCATTTTTGAACAGGCGACATACCTAGCGCAGAGTGGGTGCGTTTGTGATACACGTTTACAATATAGTTAATCAGCCACGTTTCAAATTTATCTAAGGTCATTACCGCTTCTGCTTCCGATTGATAGGt                                                                                                                                                                                                           >  1:130403/1‑251 (MQ=255)                                cATTATGCTGAATAGTTCGTTTTTCGCTTGGCAAGAAATCGAGTAATAACGTTTGTTCATCAACAGGCATTTGCGGATAACCACAACCGACATGATCTTTATCACCAAAAATCCCCAATCGCCATTTTTGAACAGGCGACATACCTAGCGCAGAGTGGGTGCGTTTGTGATACACGTTTACAATATAGTTAATCAGCCACGTTTCAAATTTATCTAAGGTCATTACCGCTTCTGCTTCCGATTGATAGGTa                                                                                                                                                                                                          >  1:640183/1‑251 (MQ=255)                                 aTTATGCTGAATAGTTCGTTTTTCGCTTGGTAAGAAATCGAGTAATAACGTTTGTTCATCAACAGGCATTTGCGGATAACCACAACCGACATGATCTTTATCACCAAAAATCCCCAATCGCCATTTTTGAACAGGCGACATACCTAGCGCAGAGTGGGTGCGTTTGTGATACACGTTTACAATATAGTTAATCAGCCACGTTTCAAATTTATCTAAGGTCATTACCGCTTCTGCTTCCGATTGATAGGTAt                                                                                                                                                                                                         <  1:40904/251‑1 (MQ=255)                                 aTTATGCTGAATAGTTCGTTTTTCGCTTGGTAAGAAATCGAGTAATAACGTTTGTTCATCAACAGGCATTTGCGGATAACCACAACCGACATGATCTTTATCACCAAAAATCCCCAATCGCCATTTTTGAACAGGCGACATACCTAGCGCAGAGTGGGTGCGTTTGTGATACACGTTTACAATATAGTTAATCAGCCACGTTTCAAATTTATCTAAGGTCATTACCGCTTCTGCTTCCGATTGATAGGTAt                                                                                                                                                                                                         <  1:777554/251‑1 (MQ=255)                                 aTTATGCTGAATAGTTCGTTTTTCGCTTGGTAAGAAATCGAGTAATAACGTTTGTTCATCAACAGGCATTTGCGGATAACCACAACCGACATGATCTTTATCACCAAAAATCCCCAATCGCCATTTTTGAACAGGCGACATACCTAGCGCAGAGTGGGTGCGTTTGTGATACACGTTTACAATATAGTTAATCAGCCACGTTTCAAATTTATCTAAGGTCATTACCGCTTCTGCTTCCGATTGATAGGTAt                                                                                                                                                                                                         >  1:614614/1‑251 (MQ=255)                                    aTGCTGAATAGTTCGTTTTTCGCTTGGTAAGAAATCGAGTAATAACGTTTGTTCATCAACAGGCATTTGCGGATAACCACAACCGACATGATCTTTATCACCAAAAATCCCCAATCGCCATTTTTGAACAGGCGACATACCTAGCGCAGAGTGGGTGCGTTTGTGATACACGTTTACAATATAGTTAATCAGCCACGTTTCAAATTTATCTAAGGTc                                                                                                                                                                                                                                        <  1:893362/217‑1 (MQ=255)                                      gCTGAATAGTTCGTTTTTCGCTTGGTAAGAAATCGAGTAATAACGTTTGTTCATCAACAGGCATTTGCGGATAACCACAACCGACATGATCTTTATCACCAAAAATCCCCAATCGCCATTTTTGAACAGGCGACATACCTAGCGCAGAGTGGGTGCGTTTGTGATACACGTTTACAATATAGTTAATCAGCCACGTTTCAAATTTATCTAAGGTCATTACCGCTTCTGCTTCCGATTGATAGGTAtcttt                                                                                                                                                                                                     >  1:338745/1‑250 (MQ=255)                                      gCTGAATAGTTCGTTTTTCGCTTGGTAAGAAATCGAGTAATAACGTTTGTTCATCAACAGGCATTTGCGGATAACCACAACCGACATGATCTTTATCACCAAAAATCCCCAATCGCCATTTTTGAACAGGCGACATACCTAGCGCAGAGTGGGTGCGTTTGTGATACACGTTTACAATATAGTTAATCAGCCACGTTTCAAATTTATCTAAGGTCATTACCGCTTCTGCTTCCGATTGATAGGTAtcttt                                                                                                                                                                                                     >  1:517160/1‑250 (MQ=255)                                       cTGAATAGTTCGTTTTTCGCTTGGTAAGAAATCGAGTAATAACGTTTGTTCATCAACAGGCATTTGCGGATAACCACAACCGACATGATCTTTATCACCAAAAATCCCCAATCGCCATTTTTGAACAGGCGACATACCTAGCGCAGAGTGGGTGCGTTTGTGATACACGTTTACAATATAGTTAATCAGCCACGTTTCAAATTTATCTAAGGTCATTACCGCTTCTGCTTCCGATTGATAGGTAtctttct                                                                                                                                                                                                   <  1:220295/251‑1 (MQ=255)                                                  gTTTTTCGCTTGGTAAGAAATCGAGTAATAACGTTTGTTCATCAACAGGCATTTGCGGATAACCACAACCGACATGATCTTTATCACCAAAAATCCCCAATCGCCATTTTTGAACAGGCGACATACCTAGCGCAGAGTGGGTGCGTTTGTGATACACGTTTACAATATAGTTAATCAGCCACGTTTCAAATTTATCTAAGGTCATTACCGCTTCTGCTTCCGATTGATAGGTATCTTTCTCTTTCGTAttt                                                                                                                                                                                        >  1:621593/1‑251 (MQ=255)                                                  gTTTTTCGCTTGGTAAGAAATCGAGTAATAACGTTTGTTCATCAACAGGCATTTGCGGATAACCACAACCGACATGATCTTTATCACCAAAAATCCCCAATCGCCATTTTTGAACAGGCGACATACCTAGCGCAGAGTGGGTGCGTTTGTGATACACGTTTACAATATAGTTAATCAGCCACGTTTCAAATTTATCTAAGGTCATTACCGCTTCTGCTTCCGATTGATAGGTATCTTTCTCTTTCGTAttt                                                                                                                                                                                        >  1:223973/1‑251 (MQ=255)                                                     tttCGCTTGGTAAGAAATCGAGTAATAACGTTTGTTCATCAACAGGCATTTGCGGATAACCACAACCGACATGATCTTTATCACCAAAAATCCCCAATCGCCATTTTTGAACAGGCGACATACCTAGCGCAGAGTGGGTGCGTTTGTGATACACGTTTACAATATAGTTAATCAGCCACGTTTCAAATTTATCTAAGGTCATTACCGCTTCTGCTTCCGATTGATAGGTATCTTTCTCTTTCGTATTTgag                                                                                                                                                                                     <  1:461013/251‑1 (MQ=255)                                                              gTAAGAAATCGAGTAATAACGTTTGTTCATCAACAGGCATTTGCGGATAACCACAACCGACATGATCTTTATCACCAAAAATCCCCAATCGCCATTTTTGAACAGGCGACATACCTAGCGCAGAGTGGGTGCGTTTGTGATACACGTTTACAATATAGTTAATCAGCCACGTTTCAAATTTATCTAAGGTCATTACCGCTTCTGCTTCCGATTGATAGGTATCTTTCTCTTTCGTATTTGAGAATGTTGTa                                                                                                                                                                            >  1:341182/1‑251 (MQ=255)                                                               tAAGAAATCGAGTAATAACGTTTGTTCATCAACAGGCATTTGCGGATAACCACAACCGACATGATCTTTATCACCAAAAATCCCCAATCGCCATTTTTGAACAGGCGACATACCTAGCGCAGAGTGGGTGCGTTTGTGATACACGTTTACAATATAGTTAATCAGCCACGTTTCAAATTTATCTAAGGTCATTACCGCTTCTGCTTCCGATTGATAGGTATCTTTCTCTTTCGTATTTGAGAATGTTGTAc                                                                                                                                                                           <  1:390091/251‑1 (MQ=255)                                                               tAAGAAATCGAGTAATAACGTTTGTTCATCAACAGGCATTTGCGGATAACCACAACCGACATGATCTTTATCACCAAAAATCCCCAATCGCCATTTTTGAACAGGCGACATACCTAGCGCAGAGTGGGTGCGTTTGTGATACACGTTTACAATATAGTTAATCAGCCACGTTTCAAATTTATCTAAGGTCATTACCGCTTCTGCTTCCGATTGATAGGTATCTTTCTCTTTCGTATTTGAGAATGTTGTAc                                                                                                                                                                           <  1:887938/251‑1 (MQ=255)                                                                        gAGTAATAACGTTTGTTCATCAACAGGCATTTGCGGATAACCACAACCGACATGATCTTTATCACCAAAAATCCCCAATCGCCATTTTTGAACAGGCGACATACCTAGCGCAGAGTGGGTGCGTTTGTGATACACGTTTACAATATAGTTAATCAGCCACGTTTCAAATTTATCTAAGGt                                                                                                                                                                                                                                         <  1:554274/180‑1 (MQ=255)                                                                            aataaCGTTTGTTCATCAACAGGCATTTGCGGATAACCACAACCGACATGATCTTTATCACCAAAAATCCCCAATCGCCATTTTTGAACAGGCGACATACCTAGCGCAGAGTGGGTGCGTTTGTGATACACGTTTACAATATAGTTAATCAGCCACGTTTCAAATTTATCTAAGGTCATTACCGCTTCTGCTTCCGATTGATAGGTATCTTTCTCTTTCGTATTTGAGAATGTTGTACCACTCAAACCGTg                                                                                                                                                              <  1:86517/251‑1 (MQ=255)                                                                               aaCGTTTGTTCATCAACAGGCATTTGCGGATAACCACAACCGACATGATCTTTATCACCAAAAATCCCCAATCGCCATTTTTGAACAGGCGACATACCTAGCGCAGAGTGGGTGCGTTTGTGATACACGTTTACAATATAGTTAATCAGCCACGTTTCAAATTTATCTAAGGTCATTACCGCTTCTGCTTCCGATTGATAGGTATCTTTCTCTTTCGTATTTGAGAATGTTGTACCACTCAAACCGTGAAc                                                                                                                                                           <  1:181836/251‑1 (MQ=255)                                                                                      gTTCATCAACAGGCATTTGCGGATAACCACAACCGACATGATCTTTATCACCAAAAATCCCCAATCGCCATTTTTGAACAGGCGACATACCTAGCGCAGAGTGGGTGCGTTTGTGATACACGTTTACAATATAGTTAATCAGCCACGTTTCAAATTTATCTAAGGTCATTACCGCTTCTGCTTCCGATTGATAGGTATCTTTCTCTTTCGTATTTGAGAATGTTGTACGACTCAAACCGTGAACTTCttt                                                                                                                                                     >  1:551421/1‑250 (MQ=255)                                                                                         catcaACAGGCATTTGCGGATAACCACAACCGACATGATCTTTATCACCAAAAATCCCCAATCGCCATTTTTGAACAGGCGACATACCTAGCGCAGAGTGGGTGCGTTTGTGATACACGTTTACAATATAGTTAATCAGCCACGTTTCAAATTTATCTAAGGTCATTACCGCTTCTGCTTCCGATTGATAGGTATCTTTCTCTTTCGTATTTGAGAATGTTGTACCACTCAAACCGTGAACTTCTTTCATa                                                                                                                                                 >  1:708459/1‑251 (MQ=255)                                                                                                         cGGATAACCACAACCGACATGATCTTTATCACCAAAAATCCCCAATCGCCATTTTTGAACAGGCGACATACCTAGCGCAGAGTGGGTGCGTTTGTGATACACGTTTACAATATAGTTAATCAGCCACGTTTCAAATTTATCTAAGGTCATTACCGCTTCTGCTTCCGATTGATAGGTATCTTTCTCTTTCGTATTTGAGAATGTTGTACCACTCAAACCGTGAACTTCTTTCATAAACGTACCGATTaca                                                                                                                                  <  1:9277/250‑1 (MQ=255)                                                                                                            aTAACCACAACCGACATGATCTTTATCACCAAAAATCCCCAATCGCCATTTTTGAACAGGCGACATACCTAGCGCAGAGTGGGTGCGTTTGTGATACACGTTTACAATATAGTTAATCAGCCACGTTTCAAATTTATCTAAGGTCATTACCGCTTCTGCTTCCGATTGATAGGTATCTTTCTCTTTCGTATTTGAGAATGTTGTACCACTCAAACCGTGAACTTCTTTCATAAACGTACCGATTACACGc                                                                                                                               <  1:688661/250‑1 (MQ=255)                                                                                                               aCCACAACCGACATGATCTTTATCACCAAAAATCCCCAATCGCCATTTTTGAACAGGCGACATACCTAGCGCAGAGTGGGTGCGTTTGTGATACACGTTTACAATATAGTTAATCAGCCACGTTTCAAATTTATCTAAGGTCATTACCGCTTCTGCTTCCGATTGATAGGTATCTTTCTCTTTCGTATTTgag                                                                                                                                                                                     <  1:670233/193‑1 (MQ=255)                                                                                                                       cGACATGATCTTTATCACCAAAAATCCCCAATCGCCATTTTTGAACAGGCGACATACCTAGCGCAGAGTGGGTGCGTTTGTGATACACGTTTACAATATAGTTAATCAGCCACGTTTCAAATTTATCTAAGGTCATTACCGCTTCTGCTTCCGATTGATAGGTATCTTTCTCTTTCGTATTTGAGAATGTTGTACCACTCAAACCGTGAACTTCTTTCATAAACGTACCGATTACACGCTCAATGTGTCCg                                                                                                                   >  1:850282/1‑251 (MQ=255)                                                                                                                             gATCTTTATCACCAAAAATCCCCAATCGCCATTTTTGAACAGGCGACATACCTAGCGCAGAGTGGGTGCGTTTGTGATACACGTTTACAATATAGTTAATCAGCCACGTTTCAAATTTATCTAAGGTCATTACCGCTTCTGCTTCCGATTGATAGGTATCTTTCTCTTTCGTATTTGAGAATGTTGTACCACTCAAACCGTGAACTTCTTTCATAAACGTACCGATTACACGCTCAATGTGTCCGCCAAAt                                                                                                             >  1:633120/1‑251 (MQ=255)                                                                                                                              aTCTTTATCACCAAAAATCCCCAATCGCCATTTTTGAACAGGCGACATACCTAGCGCAGAGTGGGTGCGTTTGTGATACACGTTTACAATATAGTTAATCAGCCACGTTTCAAATTTATCTAAGGTCATTACCGCTTCTGCTTCCGATTGATAGGTATCTTTCTCTTTCGTATTTGAGAATGTTGTACCACTCAAACCGTGAACTTCTTTCATAAACGTACCGATTACACGCTCAATGTGTCCGCCAAAtt                                                                                                            >  1:566953/1‑251 (MQ=255)                                                                                                                                                       cGCCATTTTTGAACAGGCGACATACCTAGCGCAGAGTGGGTGCGTTTGTGATACACGTTTACAATATAGTTAATCAGCCACGTTTCAAATTTATCTAAGGTCATTACCGCTTCTGCTTCCGATTGATAGGTATCTTTCTCTTTCGTATTTGAGAATGTTGTACCACTCAAACCGTGAACTTCTTTCATAAACGTACCGATTACACGCTCAATGTGTCCGCCAAATTCAGGACGACCAACAGGGCGGAACTc                                                                                   <  1:69929/251‑1 (MQ=255)                                                                                                                                                            tttttGAACAGGCGACATACCTAGCGCAGAGTGGGTGCGTTTGTGATACACGTTTACAATATAGTTAATCAGCCACGTTTCAAATTTATCTAAGGTCATTACCGCTTCTGCTTCCGATTGATAGGTATCTTTCTCTTTCGTATTTGAGAATGTTGTACCACTCAAACCGTGAACTTCTTTCATAAACGTACCGATTACACGCTCAATGTGTCCGCCAAATTc                                                                                                           <  1:526074/222‑1 (MQ=255)                                                                                                                                                                      ggCGACATACCTAGCGCAGAGTGGGTGCGTTTGTGATACACGTTTACAATATAGTTAATCAGCCACGTTTCAAATTTATCTAAGGTCATTACCGCTTCTGCTTCCGATTGATAGGTATCTTTCTCTTTCGTATTTGAGAATGTTGTAc                                                                                                                                                                           <  1:445881/148‑1 (MQ=255)                                                                                                                                                                      ggCGACATACCTAGCGCAGAGTGGGTGCGTTTGTGATACACGTTTACAATATAGTTAATCAGCCACGTTTCAAATTTATCTAAGGTCATTACCGCTTCTGCTTCCGATTGATAGGTATCTTTCTCTTTCGTATTTGAGAATGTTGTACCACTCAAACCGTGAACTTCTTTCATAAACGTACCGATTACACGCTCAATGTGTCCGCCAAATTCAGGACGACCAACAGGGCGGAACTCAAGGGCAATACCgtg                                                                    >  1:226354/1‑251 (MQ=255)                                                                                                                                                                       gCGACATACCTAGCGCAGAGTGGGTGCGTTTGTGATACACGTTTACAATATAGTTAATCAGCCACGTTTCAAATTTATCTAAGGTCATTACCGCTTCTGCTTCCGATTGATAGGTATCTTTCTCTTTCGTATTTGAGAATGTTGTACCACTCAAACCGTGAACTTCTTTCATAAACGTACCGATTACACGCTCAATGTGTCCGCCAAATTCAGGACGACCAACAGGGCGGAACTCAAGGGCAATACCg                                                                      <  1:489022/248‑1 (MQ=255)                                                                                                                                                                         gACATACCTAGCGCAGAGTGGGTGCGTTTGTGATACACGTTTACAATATAGTTAATCAGCCACGTTTCAAATTTATCTAAGGTCATTACCGCTTCTGCTTCCGATTGATAGGTATCTTTCTCTTTCGTATTTGGGAATGTTGTACCACTCAAACCGTGAACTTCTTTCATAAACGTACCGATTACACGCTCAATGTGTCCGCCAAATTCAGGACGACCAACAGGGCGGAACTCAAGGGCAATACCGtgtg                                                                  >  1:449838/1‑250 (MQ=255)                                                                                                                                                                          aCATACCTAGCGCAGAGTGGGTGCGTTTGTGATACACGTTTACAATATAGTTAATCAGCCACGTTTCAAATTTATCTAAGGTCATTACCGCTTCTGCTTCCGATTGATAGGTATCTTTCTCTTTCGTATTTGAGAATGTTGTACCACTCAAACCGTGAACTTCTTTCATAAACGTACCGATTACACGCTCAATGTGTCCGCCAAATTCAGGACGACCAACAGGGCGGAACTCAAGGGCAATAc                                                                        <  1:168342/243‑1 (MQ=255)                                                                                                                                                                              aCCTAGCGCAGAGTGGGTGCGTTTGTGATACACGTTTACAATATAGTTAATCAGCCACGTTTCAAATTTATCTAAGGTCATTACCGCTTCTGCTTCCGATTGATAGGTATCTTTCTCTTTCGTATTTGAGAATGTTGTAc                                                                                                                                                                           >  1:233173/1‑140 (MQ=255)                                                                                                                                                                              aCCTAGCGCAGAGTGGGTGCGTTTGTGATACACGTTTACAATATAGTTAATCAGCCACGTTTCAAATTTATCTAAGGTCATTACCGCTTCTGCTTCCGATTGATAGGTATCTTTCTCTTTCGTATTTGAGAATGTTGTACCACTc                                                                                                                                                                      >  1:457807/1‑145 (MQ=255)                                                                                                                                                                              aCCTAGCGCAGAGTGGGTGCGTTTGTGATACACGTTTACAATATAGTTAATCAGCCACGTTTCAAATTTATCTAAGGTCATTACCGCTTCTGCTTCCGATTGATAGGTATCTTTCTCTTTCGTATTTGAGAATGTTGTACCACTCAAACCGTGAACTTCTTTCATAAACGTACCGATTACACGCTCAATGTGTCCGCCAAATTCAGGACGACCAACAGGGCGGAACTCAAGGGc                                                                             >  1:586538/1‑234 (MQ=255)                                                                                                                                                                              aCCTAGCGCAGAGTGGGTGCGTTTGTGATACACGTTTACAATATAGTTAATCAGCCACGTTTCAAATTTATCTAAGGTCATTACCGCTTCTGCTTCCGATTGATAGGTATCTTTCTCTTTCGTATTTGAGAATGTTGTACCACTCAAACCGTGAACTTCTTTCATAAACGTACCGATTACACGCTCAATGTGTCCGCCAAATTCAGGACGACCAACAGGGCGGAACTCAAGGGCAATACCGTGTGCCTca                                                             >  1:259948/1‑250 (MQ=255)                                                                                                                                                                               ccTAGCGCAGAGTGGGTGCGTTTGTGATACACGTTTACAATATAGTTAATCAGCCACGTTTCAAATTTATCTAAGGTCATTACCGCTTCTGCTTCCGATTGATAGGTATCTTTCTCTTTCGTATTTGAGAATGTTGTACCACTCAAACCGTGAACTTCTTTCATAAACGTACCGATTACACGCTCAATGTGTCCGCCAAATTCAGGACGACCAACAGGGCGGAACTCAAGGGCAATACCGTGTGcc                                                                >  1:630917/1‑246 (MQ=255)                                                                                                                                                                               ccTAGCGCAGAGTGGGTGCGTTTGTGATACACGTTTACAATATAGTTAATCAGCCACGTTTCAAATTTATCTAAGGTCATTACCGCTTCTGCTTCCGATTGATAGGTATCTTTCTCTTTCGTATTTGAGAATGTTGTACCACTCAAACCGTGAACTTCTTTCATAAACGTACCGATTACACGCTCAATGTGTCCGCCAAATTCAGGACGACCAACAGGGCGGAACTCAAGGGCAATACCGTGTGCCTcaca                                                           >  1:270249/1‑251 (MQ=255)                                                                                                                                                                                 tAGCGCAGAGTGGGTGCGTTTGTGATACACGTTTACAATATAGTTAATCAGCCACGTTTCAAATTTATCTAAGGTCATTACCGCTTCTGCTTCCGATTGATAGGTATCTTTCTCTTTCGTATTTGAGAATGTTGTACCACTCAAACCGTGAACTTCTTTCATAAACGTACCGATTACACGCTCAATGTGTCCGCCAAATTCAGGACGACCAACAGGGCGGAACTCAAGGGCAATACCGTGTGCCTCACAAg                                                         <  1:618188/251‑1 (MQ=255)                                                                                                                                                                                 tAGCGCAGAGTGGGTGCGTTTGTGATACACGTTTACAATATAGTTAATCAGCCACGTTTCAAATTTATCTAAGGTCATTACCGCTTCTGCTTCCGATTGATAGGTATCTTTCTCTTTCGTATTTGAGAATGTTGTACCACTCAAACCGTGAACTTCTTTCATAAACGTACCGATTACACGCTCAATGTGTCCGCCAAATTCAGGACGACCAACAGGGCGGAACTCAAGGGCAATACCGTGTGCCTCACAAg                                                         <  1:346393/251‑1 (MQ=255)                                                                                                                                                                                  aGCGCAGAGTGGGTGCGTTTGTGATACACGTTTACAATATAGTTAATCAGCCACGTTTCAAATTTATCTAAGGTCATTACCGCTTCTGCTTCCGATTGATAGGTATCTTTCTCTTTCGTATTTgag                                                                                                                                                                                     >  1:549900/1‑126 (MQ=255)                                                                                                                                                                                   gcgcAGAGTGGGTGCGTTTGTGATACACGTTTACAATATAGTTAATCAGCCACGTTTCAAATTTATCTAAGGTCATTACCGCTTCTGCTTCCGATTGATAGGTATCTTTCTCTTTCGTATTTGAGAAt                                                                                                                                                                                  >  1:156565/1‑128 (MQ=255)                                                                                                                                                                                   gcgcAGAGTGGGTGCGTTTGTGATACACGTTTACAATATAGTTAATCAGCCACGTTTCAAATTTATCTAAGGTCATTACCGCTTCTGCTTCCGATTGATAGGTATCTTTCTCTTTCGTATTTGAGAATGTTGTACCACTCAAACCGTGAACTTCTTTCATAAACGTACCGATTACACGCTCAATGTGTCCGCCAAATTCAGGACGACCAACAGGGCGGAACTCAAGGGCAATACCGTGTGCCTCACAAGAt                                                       >  1:614329/1‑251 (MQ=255)                                                                                                                                                                                         agTGGGTGCGTTTGTGATACACGTTTACAATATAGTTAATCAGCCACGTTTCAAATTTATCTAAGGTCATTACCGCTTCTGCTTCCGATTGATAGGTATCTTTCTCTTTCGTATTTGAGAATGTTGTACCACTCAAACCGTGAACTTCTTTCATAAACGTACCGATTACACGCTCAATGTGTCCGCCAAATTCAGGACGACCAACAGGGCGGAACTCAAGGGCAATACCGTGTGCCTCACAAGATTTGGa                                                  <  1:268477/250‑1 (MQ=255)                                                                                                                                                                                           tGGGTGCGTTTGTGATACACGTTTACAATATAGTTAATCAGCCACGTTTCAAATTTATCTAAGGTCATTACCGCTTCTGCTTCCGATTGATAGGTATCTTTCTCTTTCGTATTTGAGAATGTTGTACCACTCAAACCGTGAACTTCTTTCATAAACGTACCGATTACACGCTCAATGTGTCCGCCAAATTCAGGACGACCAACAGGGCGGAACTCAAGGGCAATACCGTGTGCCTCACAAGATTTGGAt                                                 <  1:775355/249‑1 (MQ=255)                                                                                                                                                                                            gggTGCGTTTGTGATACACGTTTACAATATAGTTAATCAGCCACGTTTCAAATTTATCTAAGGTCATTACCGCTTCTGCTTCCGATTGATAGGTATCTTTCTCTTTCGTATTTGAGAATGTTGTACCACTCAAACCGTGAACTTCTTTCATAAACGTACCGATTACACGCTCAATGTGTCCGCCAAATTCAGGACGACCAACAGGGCGGAACTCAAg                                                                                >  1:269108/1‑217 (MQ=255)                                                                                                                                                                                            gggTGCGTTTGTGATACACGTTTACAATATAGTTAATCAGCCACGTTTCAAATTTATCTAAGGTCATTACCGCTTCTGCTTCCGATTGATAGGTATCTTTCTCTTTCGTATTTGAGAATGTTGTACCACTCAAACCGTGAACTTCTTTCATAAACGTACCGATTACACGCTCAATGTGTCCGCCAAATTCAGGACGACCAACAGGGCGGAACTCAAGGGCAATACCGTGTGCCTCACAAGATTTGGATaaa                                              >  1:192337/1‑251 (MQ=255)                                                                                                                                                                                              gTGCGTTTGTGATACACGTTTACAATATAGTTAATCAGCCACGTTTCAAATTTATCTAAGGTCATTACCGCTTCTGCTTCCGATTGATAGGTATCTTTCTCTTTCGTATTTGAGAATGTTGTACCACTc                                                                                                                                                                      <  1:617782/129‑1 (MQ=255)                                                                                                                                                                                                  gTTTGTGATACACGTTTACAATATAGTTAATCAGCCACGTTTCAAATTTATCTAAGGTCATTACCGCTTCTGCTTCCGATTGATAGGTATCTTTCTCTTTCGTATTTGAGAATGTTGTACCACTCAAACCGTGAACTTCTTTCATAAACGTACCGATTACACGCTCAATGTGTCCGCCAAATTCAGGACGACCAACAGGGCGGAACTCAAGGGCAATACCGTGTGCCTCACAAGATTTGGATAAATCCAAt                                        >  1:28037/1‑251 (MQ=255)                                                                                                                                                                                                   tttGTGATACACGTTTACAATATAGTTAATCAGCCACGTTTCAAATTTATCTAAGGTCATTACCGCTTCTGCTTCCGATTGATAGGTATCTTTCTCTTTCGTATTTGAGAATGTTGTACCACTCAAACCGTGAACTTCTTTCATAAACGTACCGATTACACGCTCAATGTGTCCGCCAAATTCAGGACGACCAAc                                                                                               <  1:10717/195‑1 (MQ=255)                                                                                                                                                                                                   tttGTGATACACGTTTACAATATAGTTAATCAGCCACGTTTCAAATTTATCTAAGGTCATTACCGCTTCTGCTTCCGATTGATAGGTATCTTTCTCTTTCGTATTTGAGAATGTTGTACCACTCAAACCGTGAACTTCTTTCATAAACGTACCGATTACACGCTCAATGTGTCCGCCAAATTCAGGACGACCAACAGGGCGGAACTCAAGGGc                                                                             >  1:375724/1‑213 (MQ=255)                                                                                                                                                                                                    ttGTGATACACGTTTACAATATAGTTAATCAGCCACGTTTCAAATTTATCTAAGGTCATTACCGCTTCTGCTTCCGATTGATAGGTATCTTTCTCTTTCGTATTTGAGAATGTTGTACCACTCAAACCGTGAACTTCTTTCATAAACGTACCGATTACACGCTCAATGTGTCCGCCAAATTCAGGACGACCAACAGGGCGGAACTCAAGGGCAATACCGTGTGCCTCACAAGATTTGGATAAATCCAATGa                                      <  1:310184/251‑1 (MQ=255)                                                                                                                                                                                                            cacGTTTACAATATAGTTAATCAGCCACGTTTCAAATTTATCTAAGGTCATTACCGCTTCTGCTTCCGATTGATAGGTATCTTTCTCTTTCGTATTTGAGAATGTTGTACCACTCAAACCGTGAACTTCTTTCATAAACGTACCGATTACACGCTCAATGTGTCCGCCAAATTCAGGACGACCAACAg                                                                                             >  1:85339/1‑188 (MQ=255)                                                                                                                                                                                                              cGTTTACAATATAGTTAATCAGCCACGTTTCAAATTTATCTAAGGTCATTACCGCTTCTGCTTCCGATTGATAGGTATCTTTCTCTTTCGTATTTGAGAATGTTGTACCACTCAAACCGTGAACTTCTTTCATAAACGTACCGATTACACGCTCAATGTGTCCGCCAAATTCAGGACGACCAACAGGGCGGAACTCAAGGGc                                                                             >  1:876561/1‑202 (MQ=255)                                                                                                                                                                                                              cGTTTACAATATAGTTAATCAGCCACGTTTCAAATTTATCTAAGGTCATTACCGCTTCTGCTTCCGATTGATAGGTATCTTTCTCTTTCGTATTTGAGAATGTTGTACCACTCAAACCGTGAACTTCTTTCATAAACGTACCGATTACACGCTCAATGTGTCCGCCAAATTCAGGACGACCAACAGGGCGGAACTCAAGGGCAATACCGTGTGCCTCACAAGATTTGGATAAATCCAATGAACGGAAGt                              >  1:800498/1‑249 (MQ=255)                                                                                                                                                                                                               gTTTACAATATAGTTAATCAGCCACGTTTCAAATTTATCTAAGGTCATTACCGCTTCTGCTTCCGATTGATAGGTATCTTTCTCTTTCGTATTTGAGAATGTTGTACCACTCAAACCGTGAACTTCTTTCATAAACGTACCGATTACACGCTCAATGTGTCCGCCAAATTCAGGACGACCAACAGGGCGGAACTCAAGGGCAATAc                                                                        <  1:456458/206‑1 (MQ=255)                                                                                                                                                                                                                      atatAGTTAATCAGCCACGTTTCAAATTTATCTAAGGTCATTACCGCTTCTGCTTCCGATTGATAGGTATCTTTCTCTTTCGTATTTGAGAATGTTGTACCACTCAAACCg                                                                                                                                                                >  1:13396/1‑111 (MQ=255)                                                                                                                                                                                                                      atatAGTTAATCAGCCACGTTTCAAATTTATCTAAGGTCATTACCGCTTCTGCTTCCGATTGATAGGTATCTTTCTCTTTCGTATTTGAGAATGTTGTACCACTCAAACCGTGAACTTCTTTCATAAACGTACCGATTaca                                                                                                                                  >  1:641560/1‑141 (MQ=255)                                                                                                                                                                                                                      atatAGTTAATCAGCCACGTTTCAAATTTATCTAAGGTCATTACCGCTTCTGCTTCCGATTGATAGGTATCTTTCTCTTTCGTATTTGAGAATGTTGTACCACTCAAACCGTGAACTTCTTTCATAAACGTACCGATTACACGCTCAAtgtg                                                                                                                       >  1:843299/1‑152 (MQ=255)                                                                                                                                                                                                                      atatAGTTAATCAGCCACGTTTCAAATTTATCTAAGGACATTACCGCTTCTGCTTCCGATTGATAGGTATCTTTCTCTTTCGTATTTGAGAATGTTGTACCACTCAAACCGTGAACTTCTTTCATAAACGTACCGATTACACGCTCAATGTGTCCGCCAAATTCAGGACGACCAACAGGGCGGAACTCAAGGGCAATAc                                                                        >  1:518777/1‑199 (MQ=255)                                                                                                                                                                                                                       tataGTTAATCAGCCACGTTTCAAATTTATCTAAGGTCATTACCGCTTCTGCTTCCGATTGATAGGTATCTTTCTCTTTCGTATTTGAGAATGTTGTACCACTCAAACCGTGAACTTCTTTCATAAACGTACCGATTACACGCTCAATGTGTCCGCCAAATTCAGGACGACCAACAGGGCGGAACTCAAGGGCAATACCGTGTGCCTCACAAGATTTGGATAAATCCAATGAACGGAAGTCTGCTCCATTa                   <  1:225951/251‑1 (MQ=255)                                                                                                                                                                                                                       tataGTTAATCAGCCACGTTTCAAATTTATCTAAGGTCATTACCGCTTCTGCTTCCGATTGATAGGTATCTTTCTCTTTCGTATTTGAGAATGTTGTACCACTCAAACCGTGAACTTCTTTCATAAACGTACCGATTACACGCTCAATGTGTCCGCCAAATTCAGGACGACCAACAGGGCGGAACTCAAGGGCAATACCGTGTGCCTCACAAGATTTGGATAAATCCAATGAACGGAAGTCTGCTCCATTa                   <  1:834098/251‑1 (MQ=255)                                                                                                                                                                                                                       tataGTTAATCAGCCACGTTTCAAATTTATCTAAGGTCATTACCGCTTCTGCTTCCGATTGATAGGTATCTTTCTCTTTCGTATTTGAGAATGTTGTACCACTCAAACCGTGAACTTCTTTCATAAACGTACCGATTACACGCTCAATGTGTCCGCCAAATTCAGGACGACCAACAGGGCGGAACTCAAGGGCAATACCGTGTGCCTCACAAGATTTGGATAAATCCAATGAACGGAAGTCTGCTCCATTa                   <  1:872676/251‑1 (MQ=255)                                                                                                                                                                                                                        ataGTTAATCAGCCACGTTTCAAATTTATCTAAGGTCATTACCGCTTCTGCTTCCGATTGATAGGTATCTTTCTCTTTCGTATTTGAGAATGTTGTACCACTCAAACCGTGAACTTCTTTCATAAACGTACCGATTACACGCTCAAtg                                                                                                                         <  1:381529/148‑1 (MQ=255)                                                                                                                                                                                                                         taGTTAATCAGCCACGTTTCAAATTTATCTAAGGTCATTACCGCTTCTGCTTCCGATTGATAGGTATCTTTCTCTTTCGTATTTGAGAATGTTGTACCACTCAAACCGTGAACTTCTTTCATAAACGTACCGATTACACGCTCAATGTGTCCGCCAAATTCAGGACGACCAACAGGGCGGAACTCAAGGGCAATACCGTGTGCCTCACAAGATTTGGATAAATCCAATGAACGGAAGTCTGCTCCATTAt                  >  1:854452/1‑250 (MQ=255)                                                                                                                                                                                                                         taGTTAATCAGCCACGTTTCAAATTTATCTAAGGTCATTACCGCTTCTGCTTCCGATTGATAGGTATCTTTCTCTTTCGTATTTGAGAATGTTGTACCACTCAAACCGTGAACTTCTTTCATAAACGTACCGATTACACGCTCAATGTGTCCGCCAAATTCAGGACGACCAACAGGGCGGAACTCAAGGGCAATACCGTGTGCCTCACAAGATTTGGATAAATCCAATGAACGGAAGTCTGCTCCATTAt                  >  1:356968/1‑250 (MQ=255)                                                                                                                                                                                                                           gTTAATCAGCCACGTTTCAAATTTATCTAAGGTCATTACCGCTTCTGCTTCCGATTGATAGGTATCTTTCTCTTTCGTATTTGAGAATGTTGTACCACTCAAACCGTGAACTTCTTTCATAAACGTACCGATTACACGCTCAATGTGTCCGCCAAATTCAGGACGACCAACAGGGCGGAACTCAAGGGCAATACCGTGTGCCTCACAAGATTTGGAt                                                 <  1:840289/217‑1 (MQ=255)                                                                                                                                                                                                                           gTTAATCAGCCACGTTTCAAATTTATCTAAGGTCATTACCGCTTCTGCTTCCGATTGATAGGTATCTTTCTCTTTCGTATTTGAGAATGTTGTACCACTCAAACCGTGAACTTCTTTCATAAACGTACCGATTACACGCTCAATGTGTCCGCCAAATTCAGGACGACCAACAGGGCGGAACTCAAGGGCAATACCGTGTGCCTCACAAGATTTGGATAACTCCAATGAACGGAAGTCTGCTCCATTATCCa               >  1:396799/1‑251 (MQ=255)                                                                                                                                                                                                                           gTTAATCAGCCACGTTTCAAATTTATCTAAGGTCATTACCGCTTCTGCTTCCGATTGATAGGTATCTTTCTCTTTCGTATTTGAGAATGTTGTACCACTCAAACCGTGAACTTCTTTCATAAACGTACCGATTACACGCTCAATGTGTCCGCCAAATTCAGGACGACCAACAGGGCGGAACTCAAGGGCAATACCGTGTGCCTCACAAGATTTGGATAAATCCAATGAACGGAAGTCTGCTCCATTATCCa               >  1:345963/1‑251 (MQ=255)                                                                                                                                                                                                                            ttAATCAGCCACGTTTCAAATTTATCTAAGGTCATTACCGCTTCTGCTTCCGATTGATAGGTATCTTTCTCTTTCGTATTTGAGAATGTTGTACCACTCAAACCGTGAACTTCTTTCATAAACGTACCGATTACACGCTCAATGTGTCCGCCAAATTCAGGACGACCAACAGGGCGGAACTCAAGGGCAATACCGTGTGCCTCACAAGATTTGGATAAATCCAATGAACGGAAGTCTGCTCCATTATcc                <  1:297560/249‑1 (MQ=255)                                                                                                                                                                                                                              aaTCAGCCACGTTTCAAATTTATCTAAGGTCATTACCGCTTCTGCTTCCGATTGATAGGTATCTTTCTCTTTCGTATTTGAGAATGTTGTACCACTCAAACCGTGAACTTCTTTCATAAACGTACCGATTACACGCTCAATGTGTCCGCCAAATTCAGGACGACCAACAGGGCGGAACTCAAGGGCAATAc                                                                        <  1:235833/191‑1 (MQ=255)                                                                                                                                                                                                                               aTCAGCCACGTTTCAAATTTATCTAAGGTCATTACCGCTTCTGCTTCCGATTGATAGGTATCTTTCTCTTTCGTATTTGAGAATGTTGTACCACTCAAACCGTGAACTTCTTTCATAAACGTACCGATTACACGCTCAATGTGTCCGCCAAATTCAGGACGACCAACAGGGCGGAACTCAAGGGCAATACCGTGTGCCTCACAAGATTTGGATAAATCCAATGAACGGAAGTCTGCTCCATTATCCACGTg           <  1:5883/251‑1 (MQ=255)                                                                                                                                                                                                                                         tttCAAATTTATCTAAGGTCATTACCGCTTCTGCTTCCGATTGATAGGTATCTTTCTCTTTCGTATTTGAGAATGTTGTACCACTCAAACCGTGAACTTCTTTCATAAACGTACCGATTACACGCTCAATGTGTCCGCCAAATTCAGGACGACCAACAGGGCGGAACTCAAGGGCAATACCGTGTGCCTCACAAGATTTGGATAAATCCAATGAACGGAAGTCTGCTCCATTATCCACGTGGATTTTAtt  <  1:483189/250‑1 (MQ=255)                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                 TCGTAGTAACGTAAACCATCAATCGTTACGCCATTATGCTGAATAGTTCGTTTTTCGCTTGGTAAGAAATCGAGTAATAACGTTTGTTCATCAACAGGCATTTGCGGATAACCACAACCGACATGATCTTTATCACCAAAAATCCCCAATCGCCATTTTTGAACAGGCGACATACCTAGCGCAGAGTGGGTGCGTTTGTGATACACGTTTACAATATAGTTAATCAGCCACGTTTCAAATTCATCTAAGGTCATTACCGCTTCTGCTTCCGATTGATAGGTATCTTTCTCTTTCGTATTTGAGAATGTTGTACCACTCAAACCGTGAACTTCTTTCATAAACGTACCGATTACACGCTCAATGTGTCCGCCAAATTCAGGACGACCAACAGGGCGGAACTCAAGGGCAATACCGTGTGCCTCACAAGATTTGGATAAATCCAATGAACGGAAGTCTGCTCCATTATCCACGTGGATTTTATT  >  1/314510‑314991

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑