Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,986,577	T→G	G245G (GGA→GGC)	unknown ←	16S rRNA (guanine(1207)‑N(2))‑methyltransferase (EC 2.1.1.172)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,986,577	0	T	G	100.0%	179.3 / NA	63	G245G (GGA→GGC)	unknown	16S rRNA (guanine(1207)‑N(2))‑methyltransferase (EC 2.1.1.172)
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (30/33);  total (30/33)

ACGTCATGACTGCCGAAATGTTGATTCAGCCAGTCGGCGTAAGGCAGAAACGCATTTGCCACGATGCGCAGCTCACCGCCCGGTTTCAAATGCCATTTTGCCTGTTTTATCAGTTCGTTAACGGCGGTGTAAGCGGTGTCAATGCCATCATGAAACGGCGGGTTGGAAATGATCAGATCAAATTTGCCGTCAATATGGGAAAACACATCACTGGCAACCACTGTTCCCTGTAGCCGATTTTCCTG‑TAAAGTGCGCTTTGCGGAAGCCAACGCCAAGGCATGAATATCTGTCATGATTAATCGGGTGTTCGGGTTGTGCCGTTGAATATAACTGCCGATCACTCCCGCGCCGCAGCCCACGTCCAACACCCCGCCTGCGATGTTTTTTAGCGTAGAAAGCAATAATGCCGTGCCGCCATCCAGTTCGGCGGCGCTGAATACGCCGGGCAACGCATAGAT  >  1/1986353‑1986810                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                           aCGTCATGACTGCCGAAATGTTGATTCAGCCAGTCGGCGTAAGGCAGAAACGCATTTGCCACGATGCGCAGCTCACCGCCCGGTTTCAAATGCCATTTTGCCTGTTTTATCAGTTCGTTAACGGCGGTGTAAGCGGTGTCAATGCCGTCATGAAAAGGCGGATTGGAAATGATCAGATCGAATTTGCCGTCAATATGGGAAAACACGTCACTGGCAACCACCGTGCCTTGCAACCGATTTTCCTG‑TAAAg                                                                                                                                                                                                                  <  1:506731/250‑1 (MQ=255) aCGTCATGACTGCCGAAATGTTGATTCAGCCAGTCGGCGTAAGGCAGAAACGCATTTGCCACGATGCGCAGCTCACCGCCCGGTTTCAAATGCCATTTTGCCTGTTTTATCAGTTCGTTAACGGCGGTGTAAGCGGTGTCAATGCCGTCATGAAAAGGCGGATTGGAAATGATCAGATCGAATTTGCCGTCAATATGGGAAAACACGTCACTGGCAACCACCGTGCCTTGCAACCGATTTTCCTG‑TAAAg                                                                                                                                                                                                                  <  1:386539/250‑1 (MQ=255)                                         aaGGCAGAAACGCATTTGCCACGATGCGCAGCTCACCGCCCGGTTTCAAATGCCATTTTGCCTGTTTTATCAGTTCGTTAACGGCGGTGTAAGCGGTGTCAATGCCGTCATGAAAAGGCGGATTGGAAATGATCAGATCGAATTTGCCGTCAATATGGGAAAACACGTCACTGGCAACCACCGTGCCTTGCAACCGATTTTCCTG‑TAAAGTGCGCTTTGCGGAAGCCAACGCCAAGGCGTGAATATCCGTc                                                                                                                                                                         <  1:626801/251‑1 (MQ=255)                                               gAAACGCATTTGCCACGATGCGCAGCTCACCGCCCGGTTTCAAATGCCATTTTGCCTGTTTTATCAGTTCGTTAACGGCGGTGTAAGCGGTGTCAATGCCGTCATGAAAAGGCGGATTGGAAATGATCAGATCGAATTTGCCGTCAATATGGGAAAACACGTCACTGGCAACCACCGTGCCTTGCAACCGATTTTCCTG‑TAAAGTGCGCTTTGCGGAAGCCAACGCCAAGGCGTGAat                                                                                                                                                                                <  1:601032/238‑1 (MQ=255)                                               gAAACGCATTTGCCACGATGCGCAGCTCACCGCCCGGTTTCAAATGCCATTTTGCCTGTTTTATCAGTTCGTTAACGGCGGTGTAAGCGGGGTCAATGCCGTCATGAAAAAGCGGATTGGGAATGATCAGATCGAATTTTCCGTCAATATGGGAAAAAACGTCACTGGCAACCACCGGGCCTTGCAACCGATTTTTCTG‑TAAAg                                                                                                                                                                                                                  >  1:77823/1‑204 (MQ=255)                                                aaaCGCATTTGCCACGATGCGCAGCTCACCGCCCGGTTTCAAATGCCATTTTGCCTGTTTTATCAGTTCGTTAACGGCGGTGTAAGCGGTGTCAATGCCGTCATGAAAAGGCGGATTGGAAATGATCAGATCGAATTTGCCGTCAATATGGGAAAACACGTCACTGGCAACCACCGTGCCTTGCAACCGATTTTCCTG‑TAAAGTGCGCTTTGCGGAAGCCAACGCCAAGGCGTGAATATCCGTCATGa                                                                                                                                                                     <  1:806713/248‑1 (MQ=255)                                                aaaCGCATTTGCCACGATGCGCAGCTCACCGCCCGGTTTCAAATGCCATTTTGCCTGTTTTATCAGTTCGTTAACGGCGGTGTAAGCGGTGTCAATGCCGTCATGAAAAGGCGGATTGGAAATGATCAGATCGAATTTGCCGTCAATATGGGAAAACACGTCACTGGCAACCACCGTGCCTTGCAACCGATTTTCCTG‑TAAAGTGCGCTTTGCGGAAGCCAACGCCAAGGCGTGAATATCCGTCATGAtt                                                                                                                                                                   <  1:762075/250‑1 (MQ=255)                                                          gCCACGATGCGCAGCTCACCGCCCGGTTTCAAATGCCATTTTGCCTGTTTTATCAGTTCGTTAACGGCGGTGTAAGCGGGGTCAATGCCGTCATGAAAAGGCGGGTTGGGAATGATCAGATCGAATTTGCCGTCAATATGGGGAAACAAGTCACTGGCAACCACCCTGCCTTGCAACCGATTTTTCTG‑TAAAATGCGCTTTGCGGAAGCCAACGCCAAGGCGGGAATATCCGTCATGATTAATCGGGGGtt                                                                                                                                                        >  1:597229/1‑251 (MQ=255)                                                              cGATGCGCAGCTCACCGCCCGGTTTCAAATGCCATTTTGCCTGTTTTATCAGTTCGTTAACGGCGGTGTAAGCGGTGTCAATGCCGTCATGAAAAGGCGGATTGGAAATGATCAGATCGAATTTGCCGTCAATATGGGAAAACACGTCACTGGCAACCACCGTGCCTTGCAACCGATTTTCCTG‑TAAAGTGCGCTTTGCGGAAGCCAACGCCAAGGCGTGAATATCCGTCATGATTAATCGGGTGTTCggg                                                                                                                                                    <  1:25843/251‑1 (MQ=255)                                                                aTGCGCAGCTCACCGCCCGGTTTCAAATGCCATTTTGCCTGTTTTATCAGTTCGTTAACGGCGGTGTAAGCGGTGTCAATGCCGTCATGAAAAGGCGGATTGGAAATGATCAGATCGAATTTGCCGTCAATATGGGAAAACACGTCACTGGCAACCACCGTGCCTTGCAACCGATTTTCCTG‑TAAAGTGCGCTTTGCGGAAGCCAACGCCAAGGCGTGAATATCCGTCATGATTAATCGGGTGTTCGGGt                                                                                                                                                   <  1:2235/250‑1 (MQ=255)                                                                aTGCGCAGCTCACCGCCCGGTTTCAAATGCCATTTTGCCTGTTTTATCAGTTCGTTAACGGCGGTGGAAGCGGGGTCAAAGGCGTCATGAAAAGGGGGGATGGGAAAGATTAAAACGAATTTGGCGTTAATATGGGGAAACACGGTACCGGGAACCACCCGGCCCTGCAGCCGATTTTTCTGTTAAAG‑GCGCCTTGCGGGAGCCAACGCCAAGGCGGGGAAATTCGTCATGATTAATCggggggttggggt                                                                                                                                                  >  1:456001/1‑241 (MQ=255)                                                                  gcgcAGCTCACCGCCCGGTTTCAAATGCCATTTTGCCTGTTTTATCAGTTCGTTAACGGCGGTGTAAGCGGTGTCAATGCCGTCATGAAAAAGCGGGTTGGAAATGATCAGATCGAATTTGCCGTCAATATGGGAAAAAACGTCACTGGGAACCACCGTGCCTTGCAACCGCTTTTCCTG‑TAAAGTGCGCTTTGCGGGAGCCAACGCCAAGGCGTGGATATCCGTCCTGATTAATctgggggtcgggg                                                                                                                                                   >  1:652145/1‑236 (MQ=255)                                                                  gcgcAGCTCACCGCCCGGTTTCAAATGCCATTTTGCCTGTTTTATCAGTTCGTTAACGGCGGTGTAAGCGGGGTCAATGCCGTCATGAAAAGGCGGATTGGGAATGATCAGATTGAATTTTCCGGCAATATGGGGAAAAACGGCAATGGCAACCACCCTGCCTTGCAACCGATTTTTCTG‑TAAAGTGCGCTTTGCGGGAGCCAACGCCCAGGGGGGAATATTCCTCATGATTAATCGGGGGTTCggggtgg                                                                                                                                                >  1:56099/1‑247 (MQ=255)                                                                     cAGCTCACCGCCCGGTTTCAAATGCCATTTTGCCTGTTTTATCAGTTCGTTAACGGCGGTGTAAGCGGTGTCAATGCCGTCATGAAAAGGCGGATTGGAAATGATCAGATCGAATTTGCCGTCAATATGGGAAAACACGTCACTGGCAACCACCGTGCCTTGCAACCGATTTTCCTG‑TAAAGTGCGCTTTGCGGAAGCCAACGCCAAGGCGTGAATATCCGTCATGATTAATCGGGTGTTCGGGTTGTGc                                                                                                                                              <  1:725967/250‑1 (MQ=255)                                                                     cAGCTCACCGCCCGGTTTCAAATGCCATTTTGCCTGTTTTATCAGTTCGTTAACGGCGGTGTAAGCGGTGTCAATGCCGTCATGAAAAGGCGGATTGGAAATGATCAGATCGAATTTGCCGTCAATATGGGAAAACACGTCACTGGCAACCACCGTGCCTTGCAACCGATTTTCCTG‑TAAAGTGCGCTTTGCGGAAGCCAACGCCAAGGCGTGAATATCCGTCATGATTAATCGGGTGTTCGGGTTGTGc                                                                                                                                              <  1:116878/250‑1 (MQ=255)                                                                       gCTCACCGCCCGGTTTCAAATGCCATTTTGCCTGTTTTATCAGTTCGTTAACGGCGGTGTAAACGGGGGCAATGCCGTCCTTAAAAAGCGGGTTGGGAAAGATCAGATCGAATTTGCCGGCCATATGGGGAAAAACGTCACTGGCCACCCCCGGGCCTTTCAACCGATTTTTCTG‑TAAAGAGCGCTTTGCGGGAGCCCACGCCAAGGCGTGAATATCCGTCCTGATTAATCGGGGGTTCGGGGTGTGctgg                                                                                                                                           >  1:889829/1‑248 (MQ=255)                                                                               cccGGTTTCAAATGCCATTTTGCCTGTTTTATCAGTTCGTTAACGGCGGTGTAAGCGGTGTCAATGCCGTCATGAAAAGGCGGATTGGAAATGATCAGATCGAATTTGCCGTCAATATGGGAAAACACGTCACTGGCAACCACCGTGCCTTGCAACCGATTTTCCTG‑TAAAGTGCGCTTTGCGGAAGCCAACGCCAAGGCGTGAATATCCGTCATGATTAATCGGGTGTTCGGGTTGTGCTGTTGAatat                                                                                                                                    <  1:294368/250‑1 (MQ=255)                                                                                 cGGTTTCAAATGCCATTTTGCCTGTTTTATCAGTTCGTTAACGGCGGTGTAAGCGGTGTCAATGCCGTCATGAAAAGGCGGATTGGAAATGATCAGATCGAATTTGCCGTCAATATGGGAAAACACGTCACTGGCAACCACCGTGCCTTGCAACCGATTTTCCTG‑TAAAGTGCGCTTTGCGGAAGCCAACGCCAAGGCGTGAATATCCGTCATGATTAATCGGGTGTTCGGGTTGTGCTGTTGAATATAAc                                                                                                                                 <  1:763838/251‑1 (MQ=255)                                                                                     ttCAAATGCCATTTTGCCTGTTTTATCAGTTCGTTAACGGCGGTGTAAGCGGGGTCAATGCCGTCCTGAAAAGGCGGGTTGGGAATGATCAGATCGAATTTTCCGTCAATATGGGGAAAAACGTCACTGGCAACCACCCTGCCTTGCAAACGATTTTCCTG‑TAAAATGCGCTTTTCGGAAGACAACGCCAAGGCGGGAATATCCGTCATGATTAAACGGGGGTTCGGGGTGTGCTGTTGAATATAAATGc                                                                                                                              >  1:830181/1‑250 (MQ=255)                                                                                       cAAATGCCATTTTGCCTGTTTTATCAGTTCGTTAACGGCGGTGTAAGCGGGGTCAAAGCCGTCATGAAAAGGGGGATTGGGAATGAAAAGATCGAATTTGCCGTCAATATGGGAAAACAAGACACTGGCAACCACCGTGCCCTGCAAACGATTTTCCTG‑TAAAGTGCGCTTTTTGGAAGCCAACGCCAAGGCGGGGATATCCTTCATGGTTAAATGGGTGGTCGGGTTGTGCTGTTGTATATAAATGccaa                                                                                                                           >  1:220091/1‑249 (MQ=255)                                                                                          aTGCCATTTTGCCTGTTTTATCAGTTCGTTAACGGCGGTGTAAGCGGTGTCAATGCCGTCATGAAAAGGCGGATTGGAAATGATCAGATCGAATTTGCCGTCAATATGGGAAAACACGTCACTGGCAACCACCGTGCCTTGCAACCGATTTTCCTG‑TAAAGTGCGCTTTGCGGAAGCCAACGCCAAGGCGTGAATATCCGTCATGATTAATCggg                                                                                                                                                            <  1:673398/215‑1 (MQ=255)                                                                                              cATTTTGCCTGTTTTATCAGTTCGTTAACGGCGGTGTAAGCGGTGTCAATGCCGTCATGAAAAGGCGGATTGGAAATGATCAGATCGAATTTGCCGTCAATATGGGAAAACACGTCACTGGCAACCACCGTGCCTTGCAACCGATTTTCCTG‑TAAAGTGCGCTTTGCGGAAGCCAACGCCAAGGCGTGAATATCCGTCATGATTAATCGGGTGTTCGGGTTGTGCTGTTGAATATAACTGCCAATCAcccc                                                                                                                    <  1:850484/251‑4 (MQ=255)                                                                                              cATTTTGCCTGTTTTATCAGTTCGTTAACGGCGGTGTAAGCGGTGTCAATGCCGTCATGAAAAGGCGGATTGGAAATGATCAGATCGAATTTGCCGTCAATATGGGAAAACACGTCACTGGCAACCACCGTGCCTTGCAACCGATTTTCCTG‑TAAAGTGCGCTTTGCGGAAGCCAACGCCAAGGCGTGAATATCCGTCATGATTAATCGGGTGTTCGGGTTGTGCTGTTGAATATAACTGCCAATCAcccc                                                                                                                    <  1:549221/251‑4 (MQ=255)                                                                                              cATTTTGCCTGTTTTATCAGTTCGTTAACGGCGGTGTAAGCGGGGTCAATGTCGTCCTGAAAAGGCGGGTTGGGAAAGATCAGATCGAATTTTTCGTCAATATGGGAAAAAACGTCACTGGCAACCACCGCGCCTTGCAAACGATTTTTCTG‑TTAAGTGCGCTTTTCGGGAGCCAAAGCCAAGGCGGGAATATCCGTCATGATTAATCGGGGGTTCGGGGTGTGCTGTTGAATATAAATGCCAATCAccc                                                                                                                     >  1:479260/1‑248 (MQ=255)                                                                                                        gTTTTATCAGTTCGTTAACGGCGGTGTAAGCGGGGTCAATGCCGTCATGAAAAGGCGGGTTGGGAAAGATCAGATCGAATTTGCCGTCAATATGGGGAAACACGTCCCTGGCAAACACCCTGCCTTGCAACCGATTTTTCTG‑TAAAGGGCGCTTTGCGGAAGCCAACGCCAAGGCGGGAATATCCGTCCTGATTAATCGGGTGTTCGGGGTGTGCTGTTGGatat                                                                                                                                    >  1:316686/1‑225 (MQ=255)                                                                                                        gTTTTATCAGTTCGTTAACGGCGGTGTAAGCGGGGTCAAAGCCGTCATGAAAAAGCGGGTTGGAAAAGAACAAATCGAATTTGCCGTCAAAAAGGGGAAAAACGTCACTGGCAAACACCGTGCCCTGCAACCGATTTTTCTG‑TAAAGAGCGCTTTGCGGAAGCCAACGCCCAGGCGTGAATATCCGGCATGATTAATCGGGGGTTCGGGGTGGGCTGTTGAATATAAATGCCAATCAACCCTGCGCCGCAg                                                                                                          >  1:482006/1‑251 (MQ=255)                                                                                                           ttATCAGTTCGTTAACGGCGGTGTAAGCGGTGTCAATGCCGTCATGAAAAGGCGGATTGGAAATGATCAGATCGAATTTGCCGTCAATATGGGAAAACACGTCACTGGCAACCACCGTGCCTTGCAACCGATTTTCCTG‑TAAAGTGCGCTTTGCGGAAGCCAACGCCAAGGCGTGAATATCCGTCATGATTAATCGGGTGTTCGGGTTGTGCTGTTGAATATAACTGCCAATCACCCCTGCGCCGCAGccc                                                                                                       <  1:358242/251‑1 (MQ=255)                                                                                                            tATCAGTTCGTTAACGGCGGTGTAAGCGGGGTCAATGCCGTCCTGAAAAAGCGGGTTGGGAATGATCAGATCGAATTTTCCGTCAATATTGGGAAACACGTCACTGGCAACCACCCTGCCCTGCAACCGATTTTTCTT‑TAAAATGCGCTTTTCGGGAGCCAACGCCCAGGCGGGGATATCCCTCCTGATTAATCTGGGGTTCGGGGTTTGCTGTTGAATATAAATGCCCATCACCCCCGcgcccccgccc                                                                                                       >  1:140971/1‑243 (MQ=255)                                                                                                                 gTTCGTTAACGGCGGTGTAAGCGGGGTCAATGCCGTCATGAAAAAGCGGATTGGGAATGATCAGATCGAATTTGCCGTCCATATGGGGAAACACGTCACTGGCAACCACCCTGCCTTGCAACCGATTTTCCTG‑TAAAGTGCGCTTTGCGGGAGCCAACGCCCAGGCGTGAATATCCGTCATGATTAATCGGGGGTTCGGGTTGTGCTGTTGAATATAACTGCCAATCACCCCCGCGCCGCAGCCCCCGTc                                                                                                  >  1:849997/1‑250 (MQ=255)                                                                                                                      ttAACGGCGGTGTAAGCGGTGTCAATGCCGTCATGAAAAGGCGGATTGGAAATGATCAGATCGAATTTGCCGTCAATATGGGAAAACACGTCACTGGCAACCACCGTGCCTTGCAACCGATTTTCCTG‑TAAAGTGCGCTTTGCGGAAGCCAACGCCAAGGCGTGAATATCCGTCATGATTAATCGGGTGTTCGGGTTGTGCTGTTGAATATAACTGCCAATCACCCCTGCGCCGCAGCCCACGTCCAacac                                                                                            <  1:362688/251‑1 (MQ=255)                                                                                                                      ttAACGGCGGTGTAAGCGGTGTCAATGCCGTCATGAAAAGGCGGATTGGAAATGATCAGATCGAATTTGCCGTCAATATGGGAAAACACGTCACTGGCAACCACCGTGCCTTGCAACCGATTTTCCTG‑TAAAGTGCGCTTTGCGGAAGCCAACGCCAAGGCGTGAATATCCGTCATGATTAATCGGGTGTTCGGGTTGTGCTGTTGAATATAACTGCCAATCACCCCTGCGCCGCAGCCCACGTCCAacac                                                                                            <  1:86979/251‑1 (MQ=255)                                                                                                                          cggcggTGTAAGCGGTGTCAATGCCGTCATGAAAAGGCGGATTGGAAATGATCAGATCGAATTTGCCGTCAATATGGGAAAACACGTCACTGGCAACCACCGTGCCTTGCAACCGATTTTCCTG‑TAAAGTGCGCTTTGCGGAAGCCAACGCCAAGGCGTGAATATCCGTCATGATTAATCGGGTGTTCGGGTTGTGCTGTTGAATATAACTg                                                                                                                               >  1:323483/1‑212 (MQ=255)                                                                                                                               gtgtAAGCGGTGTCAATGCCGTCATGAAAAGGCGGATTGGAAATGATCAGATCGAATTTGCCGTCAATATGGGAAAACACGTCACTGGCAACCACCGTGCCTTGCAACCGATTTTCCTG‑TAAAGTgcgc                                                                                                                                                                                                             >  1:461547/1‑129 (MQ=255)                                                                                                                               gtgtAAGCGGTGTCAATGCCGTCATGAAAAGGCGGATTGGAAATGATCAGATCGAATTTGCCGTCAATATGGGAAAACACGTCACTGGCAACCACCGTGCCTTGCAACCGATTTTCCTG‑TAAAGTGCGCTTTGCGGAAGCCAACGc                                                                                                                                                                                            <  1:556513/146‑1 (MQ=255)                                                                                                                               gtgtAAGCGGTGTCAATGCCGTCATGAAAAGGCGGATTGGAAATGATCAGATCGAATTTGCCGTCAATATGGGAAAACACGTCACTGGCAACCACCGTGCCTTGCAACCGATTTTCCTG‑TAAAGTGCGCTTTGCGGAAGCCAACGCCAAGGCGTGAATATCCGTCATGATTAATCggg                                                                                                                                                            >  1:379572/1‑178 (MQ=255)                                                                                                                                tgtAAGCGGTGTCAATGCCGTCATGAAAAGGCGGATTGGAAATGATCAGATCGAATTTGCCGTCAATATGGGAAAACACGTCACTGGCAACCACCGTGCCTTGCAACCGATTTTCCTG‑TAAAGTGCGCTTTGCGGAAGCCAACGCCAAGGCGTGAATATCCGTCATGATTAATCGGGTGTTCGGGTTGTGCTGTTGAATATAACTGCCAATCAcccc                                                                                                                    <  1:440843/217‑4 (MQ=255)                                                                                                                                 gtAAGCGGTGTCAATGCCGTCATGAAAAGGCGGATTGGAAATGATCAGATCGAATTTGCCGTCAATATGGGAAAACACGTCACTGGCAACCACCGTGCCTTGCAACCGATTTTCCTG‑TAAAGTGCGCTTTGCGGAAGCCAACGCCAAGGCGTGAATATCCGTCATGATTAATCGGGTGTTCGGGTTGTGCTGTTGAATATAACTGCCAATCACCCCTGCGCCGCAGCCCACGTCCAacac                                                                                            >  1:589367/1‑240 (MQ=255)                                                                                                                                        gtgtCAATGCCGTCATGAAAAGGCGGATTGGAAATGATCAGATCGAATTTGCCGTCAATATGGGAAAACACGTCACTGGCAACCACCGTGCCTTGCAACCGATTTTCCTG‑TAAAGTGCGCTTTGCGGAAGCCAACGCCAAGGCGTGAATATCCGTCATGATTAATCGGGTGTTCGGGTTGTGCTGTTGAATATAACTGCCAATCACCCCTGCGCCGCAGCCCACGTCCAACACCTCGCCCGCGATGttttt                                                                          >  1:274544/1‑251 (MQ=255)                                                                                                                                          gtCAATGCCGTCATGAAAAGGCGGATTGGAAATGATCAGATCGAATTTGCCGTCAATATGGGAAAACACGTCACTGGCAACCACCGTGCCTTGCAACCGATTTTCCTG‑TAAAGTGCGCTTTGCGGAAGCCAACGCCAAGGCGTGAATATCCGTCATg                                                                                                                                                                      <  1:525035/157‑1 (MQ=255)                                                                                                                                          gtCAATGCCGTCATGAAAAGGCGGATTGGAAATGATCAGATCGAATTTGCCGTCAATATGGGAAAACACGTCACTGGCAACCACCGTGCCTTGCAACCGATTTTCCTG‑TAAAGTGCGCTTTGCGGAAGCCAACGCCAAGGCGTGAATATCCGTCATGAtt                                                                                                                                                                   >  1:877830/1‑160 (MQ=255)                                                                                                                                                   gtcatGAAAAGGCGGATTGGAAATGATCAGATCGAATTTGCCGTCAATATGGGAAAACACGTCACTGGCAACCACCGTGCCTTGCAACCGATTTTCCTG‑TAAAGTGCGCTTTGCGGAAGCCAACGCCAAGGCGTGAATATCCGTCATGATTAATCGGGTGTTCGGGTTGTGCTGTTGAATATAACTGCCAATCACCCCTGCGCCGCAGCCCACGTCCAACACCTCGCCCGCGATGTTTTTTAGCGTAGaaa                                                               >  1:534545/2‑251 (MQ=255)                                                                                                                                                   gtcatGAAAAGGCGGATTGGAAATGATCAGATCGAATTTGCCGTCAATATGGGAAAACACGTCACTGGCAACCACCGTGCCTTGCAACCGATTTTCCTG‑TAAAGTGCGCTTTGCGGAAGCCAACGCCAAGGCGTGAATATCCGTCATGATTAATCGGGTGTTCGGGTTGTGCTGTTGAATATAACTGCCAATCACCCCTGCGCCGCAGCCCACGTCCAACACCTCGCCCGCGATGTTTTTTAGCGTAGaaa                                                               >  1:108366/2‑251 (MQ=255)                                                                                                                                                     catGAAAAGGCGGATTGGAAATGATCAGATCGAATTTGCCGTCAATATGGGAAAACACGTCACTGGCAACCACCGTGCCTTGCAACCGATTTTCCTG‑TAAAGTGCGCTTTGCGGAAGCCAACGCCAAGGCGTGAATATCCGTCATGATTAATCGGGTGTTCGGGTTGTGCTGTTGAATATAACTGCCAATCACCCCTGCGCCGCAGCCCACGTCCAACACCTCGCCCGCGATGTTTTTTAGCGTAGAAAGc                                                             <  1:764399/251‑1 (MQ=255)                                                                                                                                                       tGAAAAGGCGGATTGGAAATGATCAGATCGAATTTGCCGTCAATATGGGAAAACACGTCACTGGCAACCACCGTGCCTTGCAACCGATTTTCCTG‑TAAAGTGCGCTTTGCGGAAGCCAACGCCAAGGCGTGAATATCCGTCATGATTAATCGGGTGTTCGGGTTGTGCTGTTGAATATAACTGCCAATCACCCCTgcgc                                                                                                               <  1:791792/199‑1 (MQ=255)                                                                                                                                                         aaaaggcggATTGGAAATGATCAGATCGAATTTGCCGTCAATATGGGAAAACACGTCACTGGCAACCACCGTGCCTTGCAACCGATTTTCCTG‑TAAAGTGCGCTTTGCGGAAGCCAACGCCAAGGCGTGAATATCCGTCATGATTAATCGGGTGTTCGGGTTGTGCTGTTGAATATAACTGCCAATCACCCCTGCGCCGCAGCCCACGTCCAACACCTCGCCCGCGATGTTTTTTAGCGTAGAAAGCaat                                                          <  1:278914/246‑1 (MQ=255)                                                                                                                                                              gcggATTGGAAATGATCAGATCGAATTTGCCGTCAATATGGGAAAACACGTCACTGGCAACCACCGTGCCTTGCAACCGATTTTCCTG‑TAAAGTGCGCTTTGCGGAAGCCAACGCCAAGGCGTGAATATCCGTCATGATTAATCGGGTGTTCGGGTTGTGCTGTTGAATATAACTGCCAATCACCCCTGCGCCGCAGCCCACGTCCAACACCTCGCCCGCGATGTTTTTTAGCGTAGAAAGCAATAAtgc                                                     <  1:142918/250‑1 (MQ=255)                                                                                                                                                                 gattGGAAATGATCAGATCGAATTTGCCGTCAATATGGGAAAACACGTCACTGGCAACCACCGTGCCTTGCAACCGATTTTCCTG‑TAAAGTGCGCTTTGCGGAAGCCAACGCCAAGGCGTGAATATCCGTCATGATTAATCGGGTGTTCGGGTTGTGCTGTTGAatat                                                                                                                                    >  1:283830/3‑168 (MQ=255)                                                                                                                                                                 gattGGAAATGATCAGATCGAATTTGCCGTCAATATGGGAAAACACGTCACTGGCAACCACCGTGCCTTGCAACCGATTTTCCTG‑TAAAGTGCGCTTTGCGGAAGCCAACGCCAAGGCGTGAATATCCGTCATGATTAATCGGGTGTTCGGGTTGTGCTGTTGAATATAACTGCCAATCACCCCTGCGCCGCAGCCCACGTCCAACACCTCGCCCGCGATGTTTTTTAGCGTAGAAAGCAATAAtgccgtg                                                 >  1:93684/3‑251 (MQ=255)                                                                                                                                                                     ggAAATGATCAGATCGAATTTGCCGTCAATATGGGAAAACACGTCACTGGCAACCACCGTGCCTTGCAACCGATTTTCCTG‑TAAAGTGCGCTTTGCGGAAGCCAACGCCAAGGCGTGAATATCCGTCATGATTAATCGGGTGTTCGGGTTGTGCTGTTGAATATAACTGCCAATCACCCCTGCGCCGCAGCCCACGTCCAACACCTCGCCCGCGATGtttt                                                                           >  1:436831/1‑221 (MQ=255)                                                                                                                                                                      gAAATGATCAGATCGAATTTGCCGTCAATATGGGAAAACACGTCACTGGCAACCACCGTGCCTTGCAACCGATTTTCCTG‑TAAAGTGCGCTTTGCGGAAGCCAACGCCAAGGCGTGAATATCCGTCATGATTAATCGGGTGTTCGGGTTGTGCTGTTGAATATAACTGCCAATCACCCCTGCGCCGCAGCCCACGTCCAACACCTCGCCCGCGATGTTTTTTAGCGTAGAAAGCAATAATGCCGTgccgc                                             >  1:619016/1‑250 (MQ=255)                                                                                                                                                                          tGATCAGATCGAATTTGCCGTCAATATGGGAAAACACGTCACTGGCAACCACCGTGCCTTGCAACCGATTTTCCTG‑TAAAGTGCGCTTTGCGGAAGCCAACGCCAAGGCGTGAATATCCGTCATGATTAATCGGGTGTTCGGGTTGTGCTGTTGAATATAACTGCCAATCACCCCTGCGCCGCAGCCCACGTCCAACACCTCGCCCGCGATGTTTTTTAGCGTAGAAAGCAATAATGCCGTGCCGCCATcc                                        <  1:706324/251‑1 (MQ=255)                                                                                                                                                                          tGATCAGATCGAATTTGCCGTCAATATGGGAAAACACGTCACTGGCAACCACCGTGCCTTGCAACCGATTTTCCTG‑TAAAGTGCGCTTTGCGGAAGCCAACGCCAAGGCGTGAATATCCGTCATGATTAATCGGGTGTTCGGGTTGTGCTGTTGAATATAACTGCCAATCACCCCTGCGCCGCAGCCCACGTCCAACACCTCGCCCGCGATGTTTTTTAGCGTAGAAAGCAATAATGCCGTGCCGCCATcc                                        <  1:893288/251‑1 (MQ=255)                                                                                                                                                                                     aaTTTGCCGTCAATATGGGAAAACACGTCACTGGCAACCACCGTGCCTTGCAACCGATTTTCCTG‑TAAAGTGCGCTTTGCGGAAGCCAACGCCAAGGCGTGAATATCCGTCATGATTAATCGGGTGTTCGGGTTGTGCTGTTGAATATAACTGCCAATCACCCCTGCGCCGCAGCCCACGTCCAACACCTCGCCCGCGATGTTTTTTAGCGTAGAAAGCAATAATGCCGTGCCGCCATCCAGCTcggcgg                              <  1:38442/250‑1 (MQ=255)                                                                                                                                                                                            cGTCAATATGGGAAAACACGTCACTGGCAACCACCGTGCCTTGCAACCGATTTTCCTG‑TAAAGTGCGCTTTGCGGAAGCCAACGCCAAGGCGTGAATATCCGTCATGATTAATCGGGTGTTCGGGTTGTGCTGTTg                                                                                                                                         >  1:848212/1‑136 (MQ=255)                                                                                                                                                                                              tCAATATGGGAAAACACGTCACTGGCAACCACCGTGCCTTGCAACCGATTTTCCTG‑TAAAGTGCGCTTTGCGGAAGCCAACGCCAAGGCGTGAATATCCGTCATGATTAATCGGGTGTTCGGGTTGTGCTGTTGAATATAACTGCCAATCACCCCTGCGCCGCAGCCCACGTCCAACACCTCGCCCGCGATGTTTTTTAGCGTAGAAAGCAATAATGCCGTGCCGCCATCCAGCTCGGCGGCGCTGAATAc                    <  1:663360/251‑1 (MQ=255)                                                                                                                                                                                               cAATATGGGAAAACACGTCACTGGCAACCACCGTGCCTTGCAACCGATTTTCCTG‑TAAAGTGCGCTTTGCGGAAGCCAACGCCAAGGCGTGAATATCCGTCATGATTAATCGGGTGTTCGGGTTGTGCTGTTGAATATAACTGCCAATCACCCCTGCGCCGCAGCCCACGTCCAACACCTCGCCCGCGATGTTTTTTAGCGTAGAAAGCAATAATGCCGTGCCGCCATCCAGCTCGGCGGCGCTGAATACg                   >  1:843908/1‑251 (MQ=255)                                                                                                                                                                                                      ggAAAACACGTCACTGGCAACCACCGTGCCTTGCAACCGATTTTCCTG‑TAAAGTGCGCTTTGCGGAAGCCAACGCCAAGGCGTGAATATCCGTCATGATTAATCGGGTGTTCGGGTTGTGCTGTTGAATATAACTGCCAATCACCCCTGCGCCGCAGCCCACGTCCAACACCTCGCCCGCGATGTTTTTTAGCGTAGAAAGCAATAATGCCGTGCCGCCATCCAGCTCGGCGGCGCTGAATAc                    >  1:360564/1‑243 (MQ=255)                                                                                                                                                                                                              cgtCACTGGCAACCACCGTGCCTTGCAACCGATTTTCCTG‑TAAAGTGCGCTTTGCGGAAGCCAACGCCAAGGCGTGAATATCCGTCATGATTAATCGGGTGTTCGGGTTGTGc                                                                                                                                              >  1:828982/3‑113 (MQ=255)                                                                                                                                                                                                                tCACTGGCAACCACCGTGCCTTGCAACCGATTTTCCTG‑TAAAGTGCGCTTTGCGGAAGCCAACGCCAAGGCGTGAATATCCGTCATGATTAATCGGGTGTTCGGGTTGTGCTGTTGAATATAACTGCCAATCACCCCTGCGCCGCAGCCCACGTCCAACACCTCGCCCGCGATGTTTTTTAGCGTAGAAAGCAATAATGCCGTGCCGCCATCCAGCTCGGCGGCGCTGAATACGCCGGGCAACGCatagat  <  1:344180/251‑1 (MQ=255)                                                                                                                                                                                                                   cTGGCAACCACCGTGCCTTGCAACCGATTTTCCTG‑TAAAGTGCGCTTTGCGGAAGCCAACGCCAAGGCGTGAATATCCGTCATGATTAATCGGGTGTTCGGGTTGTGCTGTTGAatat                                                                                                                                    <  1:357724/118‑1 (MQ=255)                                                                                                                                                                                                                     ggCAACCACCGTGCCTTGCAACCGATTTTCCTG‑TAAAGTGCGCTTTGCGGAAGCCAACGCCAAGGCGTGAATATCCGTCATGATTAATCGGGTGTTCGGGTTGTGCTGTTGAATATAACTGCCAATCACCCCTGCGCCGCAGCCCACGTCCAACAcct                                                                                          >  1:308843/1‑157 (MQ=255)                                                                                                                                                                                                                     ggCAACCACCGTGCCTTGCAACCGATTTTCCTG‑TAAAGTGCGCTTTGCGGAAGCCAACGCCAAGGCGTGAATATCCGTCATGATTAATCGGGTGTTCGGGTTGTGCTGTTGAATATAACTGCCAATCACCCCTGCGCCGCAGCCCACGTCCAACACCTCGCCCGCGATGtttttt                                                                         <  1:865054/175‑1 (MQ=255)                                                                                                                                                                                                                     ggCAACCACCGTGCCTTGCAACCGATTTTCCTG‑TAAAGTGCGCTTTGCGGAAGCCAACGCCAAGGCGTGAATATCCGTCATGATTAATCGGGTGTTCGGGTTGTGCTGTTGAATATAACTGCCAATCACCCCTGCGCCGCAGCCCACGTCCAACACCTCGCCCGCGATGTTTTTTAGCGTAGAAAGCAATAATGCCGTGCCGCCATCCAGCTCGGCGGCGCTGAATACGCCGGGCAACGCatagat  <  1:485938/246‑1 (MQ=255)                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                           ACGTCATGACTGCCGAAATGTTGATTCAGCCAGTCGGCGTAAGGCAGAAACGCATTTGCCACGATGCGCAGCTCACCGCCCGGTTTCAAATGCCATTTTGCCTGTTTTATCAGTTCGTTAACGGCGGTGTAAGCGGTGTCAATGCCATCATGAAACGGCGGGTTGGAAATGATCAGATCAAATTTGCCGTCAATATGGGAAAACACATCACTGGCAACCACTGTTCCCTGTAGCCGATTTTCCTG‑TAAAGTGCGCTTTGCGGAAGCCAACGCCAAGGCATGAATATCTGTCATGATTAATCGGGTGTTCGGGTTGTGCCGTTGAATATAACTGCCGATCACTCCCGCGCCGCAGCCCACGTCCAACACCCCGCCTGCGATGTTTTTTAGCGTAGAAAGCAATAATGCCGTGCCGCCATCCAGTTCGGCGGCGCTGAATACGCCGGGCAACGCATAGAT  >  1/1986353‑1986810

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑