| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | 1 | 1,986,559 | A→G | D251D (GAT→GAC) | unknown ← | 16S rRNA (guanine(1207)‑N(2))‑methyltransferase (EC 2.1.1.172) |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | 1 | 1,986,559 | 0 | A | G | 98.5% | 173.7 / NA | 68 | D251D (GAT→GAC) | unknown | 16S rRNA (guanine(1207)‑N(2))‑methyltransferase (EC 2.1.1.172) |
| Reads supporting (aligned to +/- strand): ref base A (1/0); new base G (30/37); total (31/37) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.56e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CACCGAATAAACCTTAAACTTGTTATTTCTCGCCAGGACGTCATGACTGCCGAAATGTTGATTCAGCCAGTCGGCGTAAGGCAGAAACGCATTTGCCACGATGCGCAGCTCACCGCCCGGTTTCAAATGCCATTTTGCCTGTTTTATCAGTTCGTTAACGGCGGTGTAAGCGGTGTCAATGCCATCATGAAACGGCGGGTTGGAAATGATCAGATCAAATTTGCCGTCAATA‑TGGGAAAACACATCACTGGCAACCACTGTTCCCTGTAGCCGATTTTCCTG‑TAAAGTGCGCTTTGCGGAAGCCAACGCCAAGGCATGAATATCTGTCATGATTAATCGGGTGTTCGGGTTGTGCCGTTGAATATAACTGCCGATCACTCCCGCGCCGCAGCCCACGTCCAACACCCCGCCTGCGATGTTTTTTAGCGTAGAAAGCAATAATGCCGTGCCGCCATCCAGTTCGGCGGCGCTGAATACG > 1/1986316‑1986793 | cACCGAATATACCTTAAACTTGTTATTTCTCGCCAGGACGTCATGACTGCCGAAATGTTGATTCAGCCAGTCGGCGTAAGGCAGAAACGCATTTGCCACGATGCGCAGCTCACCGCCCGGTTTCAAATGCCATTTTGCCTGTTTTATCAGTTCGTTAACGGCGGTGTAAGCGGTGTCAATGCCGTCATGAAAAGGCGGATTGGAAATGATCAGATCGAATTTGCCGTCAATA‑TGGGAAAACACGTCACTgg < 1:475818/251‑1 (MQ=255)cACCGAATATACCTTAAACTTGTTATTTCTCGCCAGGACGTCATGACTGCCGAAATGTTGATTCAGCCAGTCGGCGTAAGGCAGAAACGCATTTGCCACGATGCGCAGCTCACCGCCCGGTTTCAAATGCCATTTTGCCTGTTTTATCAGTTCGTTAACGGCGGTGTAAGCGGTGTCAATGCCGTCATGAAAAGGCGGATTGGAAATGATCAGATCGAATTTGCCGTCAATA‑TGGGAAAACACGTCACTgg < 1:740646/251‑1 (MQ=255) ccGAATATACCTTAAACTTGTTATTTCTCGCCAGGACGTCATGACTGCCGAAATGTTGATTCAGCCAGTCGGCGTAAGGCAGAAACGCATTTGCCACGATGCGCAGCTCACCGCCCGGTTTCAAATGCCATTTTGCCTGTTTTATCAGTTCGTTAACGGCGGTGTAAGCGGGGTCAATGCCGTCATGAAAAGGCGGGTTGGGAATGATCAGATCGAAATTGCCGTCAATA‑TGGGGAAAAACGTCACTGGCa > 1:327429/1‑251 (MQ=255) ccGAATATACCTTAAACTTGTTATTTCTCGCCAGGACGTCATGACTGCCGAAATGTTGATTCAGCCAGTCGGCGTAAGGCAGAAACGCATTTGCCACGATGCGCAGCTCACCGCCCGGTTTCAAATGCCATTTTGCCTGTTTTATCAGTTCGTTAACGGCGGTGGAAGCGGTGTCCATGCCGTCCTTAAAAAGCGGGTTGGAAATTATCAGATCGAATTTTCCGTCCATA‑TTGGAAAACACACCACTGGCa > 1:887063/1‑251 (MQ=255) tataCCTTAAACTTGTTATTTCTCGCCAGGACGTCATGACTGCCGAAATGTTGATTCAGCCAGTCGGCGTAAGGCAGAAACGCATTTGCCACGATGCGCAGCTCACCGCCCGGTTTCAAATGCCATTTTGCCTGTTTTATCAGTTCGTTAACGGCGGTGTAAGCGGTGTCAATGCCGTCATGAAAAGGCGGATTGGAAATGATCAGATCGAATTTGCCGTCAATA‑TGGGAAAACACGTCACTGGCAACCAc < 1:235068/248‑1 (MQ=255) aCCTTAAACTTGTTATTTCTCGCCAGGACGTCATGACTGCCGAAATGTTGATTCAGCCAGTCGGCGTAAGGCAGAAACGCATTTGCCACGATGCGCAGCTCACCGCCCGGTTTCAAATGCCATTTTGCCTGTTTTATCAGTTCGTTAACGGCGGTGTAAGCGGGGTCAATGCCGTCCTGGAAAGGGGGGTTGGGAATGATCAGATCGAATTTGCCGGCAATATTGGGAAAA‑AAGTCACTGGGAACCAccgg > 1:224597/1‑248 (MQ=255) cTTAAACTTGTTATTTCTCGCCAGGACGTCATGACTGCCGAAATGTTGATTCAGCCAGTCGGCGTAAGGCAGAAACGCATTTGCCACGATGCGCAGCTCACCGCCCGGTTTCAAATGCCATTTTGCCTGTTTTATCAGTTCGTTAACGGCGGTGTAAGCGGTGTCAATGCCGTCATGAAAAGGCGGATTGGAAATGATCAGATCGAATTTGCCGTCAATA‑TGGGAAAACACGTCACTGGCAACCAccgtgc < 1:836938/251‑6 (MQ=255) aTTTCTCGCCAGGACGTCATGACTGCCGAAATGTTGATTCAGCCAGTCGGCGTAAGGCAGAAACGCATTTGCCACGATGCGTAGCTCACCGCCCGGTTTCAAATGCCATTTTGCCTGTTTTATCAGTTCGTTAACGGCGGTGTAAGCGGTGTCAATGCCGTCATGAAAAGGCGGATTGGAAATGATCAGATCGAATTTGCCGTCAATA‑TGGGAAAACACGTCACTGGCAACCAccgtgccttgcaaccgat < 1:448429/251‑18 (MQ=255) cGCCAGGACGTCATGACTGCCGAAATGTTGATTCAGCCAGTCGGCGTAAGGCAGAAACGCATTTGCCACGATGCGCAGCTCACCGCCCGGTTTCAAATGCCATTTTGCCTGTTTTATCAGTTCGTTAACGGCGGTGTAAGCGGTGTCAATGCCGTCATGAAAAGGCGGATTGGAAATGATCAGATCGAATTTGCCGTCAATA‑TGGGAAAACACGTCACTGGCAACCAc < 1:630124/228‑1 (MQ=255) aCGTCATGACTGCCGAAATGTTGATTCAGCCAGTCGGCGTAAGGCAGAAACGCATTTGCCACGATGCGCAGCTCACCGCCCGGTTTCAAATGCCATTTTGCCTGTTTTATCAGTTCGTTAACGGCGGTGTAAGCGGTGTCAATGCCGTCATGAAAAGGCGGATTGGAAATGATCAGATCGAATTTGCCGTCAATA‑TGGGAAAACACGTCACTGGCAACCACCGTGCCTTGCAACCGATTTTCCTG‑TAAAg < 1:506731/250‑1 (MQ=255) aCGTCATGACTGCCGAAATGTTGATTCAGCCAGTCGGCGTAAGGCAGAAACGCATTTGCCACGATGCGCAGCTCACCGCCCGGTTTCAAATGCCATTTTGCCTGTTTTATCAGTTCGTTAACGGCGGTGTAAGCGGTGTCAATGCCGTCATGAAAAGGCGGATTGGAAATGATCAGATCGAATTTGCCGTCAATA‑TGGGAAAACACGTCACTGGCAACCACCGTGCCTTGCAACCGATTTTCCTG‑TAAAg < 1:386539/250‑1 (MQ=255) aaGGCAGAAACGCATTTGCCACGATGCGCAGCTCACCGCCCGGTTTCAAATGCCATTTTGCCTGTTTTATCAGTTCGTTAACGGCGGTGTAAGCGGTGTCAATGCCGTCATGAAAAGGCGGATTGGAAATGATCAGATCGAATTTGCCGTCAATA‑TGGGAAAACACGTCACTGGCAACCACCGTGCCTTGCAACCGATTTTCCTG‑TAAAGTGCGCTTTGCGGAAGCCAACGCCAAGGCGTGAATATCCGTc < 1:626801/251‑1 (MQ=255) gAAACGCATTTGCCACGATGCGCAGCTCACCGCCCGGTTTCAAATGCCATTTTGCCTGTTTTATCAGTTCGTTAACGGCGGTGTAAGCGGTGTCAATGCCGTCATGAAAAGGCGGATTGGAAATGATCAGATCGAATTTGCCGTCAATA‑TGGGAAAACACGTCACTGGCAACCACCGTGCCTTGCAACCGATTTTCCTG‑TAAAGTGCGCTTTGCGGAAGCCAACGCCAAGGCGTGAat < 1:601032/238‑1 (MQ=255) gAAACGCATTTGCCACGATGCGCAGCTCACCGCCCGGTTTCAAATGCCATTTTGCCTGTTTTATCAGTTCGTTAACGGCGGTGTAAGCGGGGTCAATGCCGTCATGAAAAAGCGGATTGGGAATGATCAGATCGAATTTTCCGTCAATA‑TGGGAAAAAACGTCACTGGCAACCACCGGGCCTTGCAACCGATTTTTCTG‑TAAAg > 1:77823/1‑204 (MQ=255) aaaCGCATTTGCCACGATGCGCAGCTCACCGCCCGGTTTCAAATGCCATTTTGCCTGTTTTATCAGTTCGTTAACGGCGGTGTAAGCGGTGTCAATGCCGTCATGAAAAGGCGGATTGGAAATGATCAGATCGAATTTGCCGTCAATA‑TGGGAAAACACGTCACTGGCAACCACCGTGCCTTGCAACCGATTTTCCTG‑TAAAGTGCGCTTTGCGGAAGCCAACGCCAAGGCGTGAATATCCGTCATGa < 1:806713/248‑1 (MQ=255) aaaCGCATTTGCCACGATGCGCAGCTCACCGCCCGGTTTCAAATGCCATTTTGCCTGTTTTATCAGTTCGTTAACGGCGGTGTAAGCGGTGTCAATGCCGTCATGAAAAGGCGGATTGGAAATGATCAGATCGAATTTGCCGTCAATA‑TGGGAAAACACGTCACTGGCAACCACCGTGCCTTGCAACCGATTTTCCTG‑TAAAGTGCGCTTTGCGGAAGCCAACGCCAAGGCGTGAATATCCGTCATGAtt < 1:762075/250‑1 (MQ=255) gCCACGATGCGCAGCTCACCGCCCGGTTTCAAATGCCATTTTGCCTGTTTTATCAGTTCGTTAACGGCGGTGTAAGCGGGGTCAATGCCGTCATGAAAAGGCGGGTTGGGAATGATCAGATCGAATTTGCCGTCAATA‑TGGGGAAACAAGTCACTGGCAACCACCCTGCCTTGCAACCGATTTTTCTG‑TAAAATGCGCTTTGCGGAAGCCAACGCCAAGGCGGGAATATCCGTCATGATTAATCGGGGGtt > 1:597229/1‑251 (MQ=255) ccACGATGCGCAGCTCACCGCCCGGTTTCAAATGCCATTTTGCCTGTTTTATCAGTTCGTTAACGGCGGTGTAAGCGGTGTCAATGCCGTCATGAAAAGGCGGATTGGAAATGATCAGATCGAATTTGCCGTCAATA‑TGGGAAAACACGTCACTGGCAACCAc < 1:654530/163‑1 (MQ=255) cGATGCGCAGCTCACCGCCCGGTTTCAAATGCCATTTTGCCTGTTTTATCAGTTCGTTAACGGCGGTGTAAGCGGTGTCAATGCCGTCATGAAAAGGCGGATTGGAAATGATCAGATCGAATTTGCCGTCAATA‑TGGGAAAACACGTCACTGGCAACCACCGTGCCTTGCAACCGATTTTCCTG‑TAAAGTGCGCTTTGCGGAAGCCAACGCCAAGGCGTGAATATCCGTCATGATTAATCGGGTGTTCggg < 1:25843/251‑1 (MQ=255) aTGCGCAGCTCACCGCCCGGTTTCAAATGCCATTTTGCCTGTTTTATCAGTTCGTTAACGGCGGTGTAAGCGGTGTCAATGCCGTCATGAAAAGGCGGATTGGAAATGATCAGATCGAATTTGCCGTCAATA‑TGGGAAAACACGTCACTGGCAACCACCGTGCCTTGCAACCGATTTTCCTG‑TAAAGTGCGCTTTGCGGAAGCCAACGCCAAGGCGTGAATATCCGTCATGATTAATCGGGTGTTCGGGt < 1:2235/250‑1 (MQ=255) aTGCGCAGCTCACCGCCCGGTTTCAAATGCCATTTTGCCTGTTTTATCAGTTCGTTAACGGCGGTGGAAGCGGGGTCAAAGGCGTCATGAAAAGGGGGGATGGGAAAGATTAAAACGAATTTGGCGTTAATA‑TGGGGAAACACGGTACCGGGAACCACCCGGCCCTGCAGCCGATTTTTCTGTTAAAG‑GCGCCTTGCGGGAGCCAACGCCAAGGCGGGGAAATTCGTCATGATTAATCggggggttggggt > 1:456001/1‑241 (MQ=255) gcgcAGCTCACCGCCCGGTTTCAAATGCCATTTTGCCTGTTTTATCAGTTCGTTAACGGCGGTGTAAGCGGTGTCAATGCCGTCATGAAAAAGCGGGTTGGAAATGATCAGATCGAATTTGCCGTCAATA‑TGGGAAAAAACGTCACTGGGAACCACCGTGCCTTGCAACCGCTTTTCCTG‑TAAAGTGCGCTTTGCGGGAGCCAACGCCAAGGCGTGGATATCCGTCCTGATTAATctgggggtcgggg > 1:652145/1‑236 (MQ=255) gcgcAGCTCACCGCCCGGTTTCAAATGCCATTTTGCCTGTTTTATCAGTTCGTTAACGGCGGTGTAAGCGGGGTCAATGCCGTCATGAAAAGGCGGATTGGGAATGATCAGATTGAATTTTCCGGCAATA‑TGGGGAAAAACGGCAATGGCAACCACCCTGCCTTGCAACCGATTTTTCTG‑TAAAGTGCGCTTTGCGGGAGCCAACGCCCAGGGGGGAATATTCCTCATGATTAATCGGGGGTTCggggtgg > 1:56099/1‑247 (MQ=255) cAGCTCACCGCCCGGTTTCAAATGCCATTTTGCCTGTTTTATCAGTTCGTTAACGGCGGTGTAAGCGGTGTCAATGCCGTCATGAAAAGGCGGATTGGAAATGATCAGATCGAATTTGCCGTCAATA‑TGGGAAAACACGTCACTGGCAACCACCGTGCCTTGCAACCGATTTTCCTG‑TAAAGTGCGCTTTGCGGAAGCCAACGCCAAGGCGTGAATATCCGTCATGATTAATCGGGTGTTCGGGTTGTGc < 1:725967/250‑1 (MQ=255) cAGCTCACCGCCCGGTTTCAAATGCCATTTTGCCTGTTTTATCAGTTCGTTAACGGCGGTGTAAGCGGTGTCAATGCCGTCATGAAAAGGCGGATTGGAAATGATCAGATCGAATTTGCCGTCAATA‑TGGGAAAACACGTCACTGGCAACCACCGTGCCTTGCAACCGATTTTCCTG‑TAAAGTGCGCTTTGCGGAAGCCAACGCCAAGGCGTGAATATCCGTCATGATTAATCGGGTGTTCGGGTTGTGc < 1:116878/250‑1 (MQ=255) gCTCACCGCCCGGTTTCAAATGCCATTTTGCCTGTTTTATCAGTTCGTTAACGGCGGTGTAAACGGGGGCAATGCCGTCCTTAAAAAGCGGGTTGGGAAAGATCAGATCGAATTTGCCGGCCATA‑TGGGGAAAAACGTCACTGGCCACCCCCGGGCCTTTCAACCGATTTTTCTG‑TAAAGAGCGCTTTGCGGGAGCCCACGCCAAGGCGTGAATATCCGTCCTGATTAATCGGGGGTTCGGGGTGTGctgg > 1:889829/1‑248 (MQ=255) cccGGTTTCAAATGCCATTTTGCCTGTTTTATCAGTTCGTTAACGGCGGTGTAAGCGGTGTCAATGCCGTCATGAAAAGGCGGATTGGAAATGATCAGATCGAATTTGCCGTCAATA‑TGGGAAAACACGTCACTGGCAACCACCGTGCCTTGCAACCGATTTTCCTG‑TAAAGTGCGCTTTGCGGAAGCCAACGCCAAGGCGTGAATATCCGTCATGATTAATCGGGTGTTCGGGTTGTGCTGTTGAatat < 1:294368/250‑1 (MQ=255) cGGTTTCAAATGCCATTTTGCCTGTTTTATCAGTTCGTTAACGGCGGTGTAAGCGGTGTCAATGCCGTCATGAAAAGGCGGATTGGAAATGATCAGATCGAATTTGCCGTCAATA‑TGGGAAAACACGTCACTGGCAACCACCGTGCCTTGCAACCGATTTTCCTG‑TAAAGTGCGCTTTGCGGAAGCCAACGCCAAGGCGTGAATATCCGTCATGATTAATCGGGTGTTCGGGTTGTGCTGTTGAATATAAc < 1:763838/251‑1 (MQ=255) ttCAAATGCCATTTTGCCTGTTTTATCAGTTCGTTAACGGCGGTGTAAGCGGGGTCAATGCCGTCCTGAAAAGGCGGGTTGGGAATGATCAGATCGAATTTTCCGTCAATA‑TGGGGAAAAACGTCACTGGCAACCACCCTGCCTTGCAAACGATTTTCCTG‑TAAAATGCGCTTTTCGGAAGACAACGCCAAGGCGGGAATATCCGTCATGATTAAACGGGGGTTCGGGGTGTGCTGTTGAATATAAATGc > 1:830181/1‑250 (MQ=255) cAAATGCCATTTTGCCTGTTTTATCAGTTCGTTAACGGCGGTGTAAGCGGGGTCAAAGCCGTCATGAAAAGGGGGATTGGGAATGAAAAGATCGAATTTGCCGTCAATA‑TGGGAAAACAAGACACTGGCAACCACCGTGCCCTGCAAACGATTTTCCTG‑TAAAGTGCGCTTTTTGGAAGCCAACGCCAAGGCGGGGATATCCTTCATGGTTAAATGGGTGGTCGGGTTGTGCTGTTGTATATAAATGccaa > 1:220091/1‑249 (MQ=255) aTGCCATTTTGCCTGTTTTATCAGTTCGTTAACGGCGGTGTAAGCGGTGTCAATGCCGTCATGAAAAGGCGGATTGGAAATGATCAGATCGAATTTGCCGTCAATA‑TGGGAAAACACGTCACTGGCAACCACCGTGCCTTGCAACCGATTTTCCTG‑TAAAGTGCGCTTTGCGGAAGCCAACGCCAAGGCGTGAATATCCGTCATGATTAATCggg < 1:673398/215‑1 (MQ=255) ccATTTTGCCTGTTTTATCAGTTCGTTAACGGCGGTGTAAGCGGTGTCAATGCCGTCATGAAAAGGCGGATTGGAAATGATCAGATCGAATTTGCCGTCAATA‑TGGGAAAACACGTCACTGGCAACCAc < 1:631068/129‑1 (MQ=255) cATTTTGCCTGTTTTATCAGTTCGTTAACGGCGGTGTAAGCGGTGTCAATGCCGTCATGAAAAGGCGGATTGGAAATGATCAGATCGAATTTGCCGTCAATA‑TGGGAAAACACGTCACTGGCAACCACCGTGCCTTGCAACCGATTTTCCTG‑TAAAGTGCGCTTTGCGGAAGCCAACGCCAAGGCGTGAATATCCGTCATGATTAATCGGGTGTTCGGGTTGTGCTGTTGAATATAACTGCCAATCAcccc < 1:549221/251‑4 (MQ=255) cATTTTGCCTGTTTTATCAGTTCGTTAACGGCGGTGTAAGCGGTGTCAATGCCGTCATGAAAAGGCGGATTGGAAATGATCAGATCGAATTTGCCGTCAATA‑TGGGAAAACACGTCACTGGCAACCACCGTGCCTTGCAACCGATTTTCCTG‑TAAAGTGCGCTTTGCGGAAGCCAACGCCAAGGCGTGAATATCCGTCATGATTAATCGGGTGTTCGGGTTGTGCTGTTGAATATAACTGCCAATCAcccc < 1:850484/251‑4 (MQ=255) cATTTTGCCTGTTTTATCAGTTCGTTAACGGCGGTGTAAGCGGGGTCAATGTCGTCCTGAAAAGGCGGGTTGGGAAAGATCAGATCGAATTTTTCGTCAATA‑TGGGAAAAAACGTCACTGGCAACCACCGCGCCTTGCAAACGATTTTTCTG‑TTAAGTGCGCTTTTCGGGAGCCAAAGCCAAGGCGGGAATATCCGTCATGATTAATCGGGGGTTCGGGGTGTGCTGTTGAATATAAATGCCAATCAccc > 1:479260/1‑248 (MQ=255) gTTTTATCAGTTCGTTAACGGCGGTGTAAGCGGGGTCAATGCCGTCATGAAAAGGCGGGTTGGGAAAGATCAGATCGAATTTGCCGTCAATA‑TGGGGAAACACGTCCCTGGCAAACACCCTGCCTTGCAACCGATTTTTCTG‑TAAAGGGCGCTTTGCGGAAGCCAACGCCAAGGCGGGAATATCCGTCCTGATTAATCGGGTGTTCGGGGTGTGCTGTTGGatat > 1:316686/1‑225 (MQ=255) gTTTTATCAGTTCGTTAACGGCGGTGTAAGCGGGGTCAAAGCCGTCATGAAAAAGCGGGTTGGAAAAGAACAAATCGAATTTGCCGTCAAAA‑AGGGGAAAAACGTCACTGGCAAACACCGTGCCCTGCAACCGATTTTTCTG‑TAAAGAGCGCTTTGCGGAAGCCAACGCCCAGGCGTGAATATCCGGCATGATTAATCGGGGGTTCGGGGTGGGCTGTTGAATATAAATGCCAATCAACCCTGCGCCGCAg > 1:482006/1‑251 (MQ=255) ttATCAGTTCGTTAACGGCGGTGTAAGCGGTGTCAATGCCGTCATGAAAAGGCGGATTGGAAATGATCAGATCGAATTTGCCGTCAATA‑TGGGAAAACACGTCACTGGCAACCACCGTGCCTTGCAACCGATTTTCCTG‑TAAAGTGCGCTTTGCGGAAGCCAACGCCAAGGCGTGAATATCCGTCATGATTAATCGGGTGTTCGGGTTGTGCTGTTGAATATAACTGCCAATCACCCCTGCGCCGCAGccc < 1:358242/251‑1 (MQ=255) tATCAGTTCGTTAACGGCGGTGTAAGCGGGGTCAATGCCGTCCTGAAAAAGCGGGTTGGGAATGATCAGATCGAATTTTCCGTCAATA‑TTGGGAAACACGTCACTGGCAACCACCCTGCCCTGCAACCGATTTTTCTT‑TAAAATGCGCTTTTCGGGAGCCAACGCCCAGGCGGGGATATCCCTCCTGATTAATCTGGGGTTCGGGGTTTGCTGTTGAATATAAATGCCCATCACCCCCGcgcccccgccc > 1:140971/1‑243 (MQ=255) gTTCGTTAACGGCGGTGTAAGCGGGGTCAATGCCGTCATGAAAAAGCGGATTGGGAATGATCAGATCGAATTTGCCGTCCATA‑TGGGGAAACACGTCACTGGCAACCACCCTGCCTTGCAACCGATTTTCCTG‑TAAAGTGCGCTTTGCGGGAGCCAACGCCCAGGCGTGAATATCCGTCATGATTAATCGGGGGTTCGGGTTGTGCTGTTGAATATAACTGCCAATCACCCCCGCGCCGCAGCCCCCGTc > 1:849997/1‑250 (MQ=255) ttAACGGCGGTGTAAGCGGTGTCAATGCCGTCATGAAAAGGCGGATTGGAAATGATCAGATCGAATTTGCCGTCAATA‑TGGGAAAACACGTCACTGGCAACCACCGTGCCTTGCAACCGATTTTCCTG‑TAAAGTGCGCTTTGCGGAAGCCAACGCCAAGGCGTGAATATCCGTCATGATTAATCGGGTGTTCGGGTTGTGCTGTTGAATATAACTGCCAATCACCCCTGCGCCGCAGCCCACGTCCAacac < 1:86979/251‑1 (MQ=255) ttAACGGCGGTGTAAGCGGTGTCAATGCCGTCATGAAAAGGCGGATTGGAAATGATCAGATCGAATTTGCCGTCAATA‑TGGGAAAACACGTCACTGGCAACCACCGTGCCTTGCAACCGATTTTCCTG‑TAAAGTGCGCTTTGCGGAAGCCAACGCCAAGGCGTGAATATCCGTCATGATTAATCGGGTGTTCGGGTTGTGCTGTTGAATATAACTGCCAATCACCCCTGCGCCGCAGCCCACGTCCAacac < 1:362688/251‑1 (MQ=255) cggcggTGTAAGCGGTGTCAATGCCGTCATGAAAAGGCGGATTGGAAATGATCAGATCGAATTTGCCGTCAATA‑TGGGAAAACACGTCACTGGCAACCACCGTGCCTTGCAACCGATTTTCCTG‑TAAAGTGCGCTTTGCGGAAGCCAACGCCAAGGCGTGAATATCCGTCATGATTAATCGGGTGTTCGGGTTGTGCTGTTGAATATAACTg > 1:323483/1‑212 (MQ=255) gtgtAAGCGGTGTCAATGCCGTCATGAAAAGGCGGATTGGAAATGATCAGATCGAATTTGCCGTCAATA‑TGGGAAAACACGTCACTGGCAACCACCGTGCCTTGCAACCGATTTTCCTG‑TAAAGTgcgc > 1:461547/1‑129 (MQ=255) gtgtAAGCGGTGTCAATGCCGTCATGAAAAGGCGGATTGGAAATGATCAGATCGAATTTGCCGTCAATA‑TGGGAAAACACGTCACTGGCAACCACCGTGCCTTGCAACCGATTTTCCTG‑TAAAGTGCGCTTTGCGGAAGCCAACGc < 1:556513/146‑1 (MQ=255) gtgtAAGCGGTGTCAATGCCGTCATGAAAAGGCGGATTGGAAATGATCAGATCGAATTTGCCGTCAATA‑TGGGAAAACACGTCACTGGCAACCACCGTGCCTTGCAACCGATTTTCCTG‑TAAAGTGCGCTTTGCGGAAGCCAACGCCAAGGCGTGAATATCCGTCATGATTAATCggg > 1:379572/1‑178 (MQ=255) tgtAAGCGGTGTCAATGCCGTCATGAAAAGGCGGATTGGAAATGATCAGATCGAATTTGCCGTCAATA‑TGGGAAAACACGTCACTGGCAACCACCGTGCCTTGCAACCGATTTTCCTG‑TAAAGTGCGCTTTGCGGAAGCCAACGCCAAGGCGTGAATATCCGTCATGATTAATCGGGTGTTCGGGTTGTGCTGTTGAATATAACTGCCAATCAcccc < 1:440843/217‑4 (MQ=255) gtAAGCGGTGTCAATGCCGTCATGAAAAGGCGGATTGGAAATGATCAGATCGAATTTGCCGTCAATA‑TGGGAAAACACGTCACTGGCAACCACCGTGCCTTGCAACCGATTTTCCTG‑TAAAGTGCGCTTTGCGGAAGCCAACGCCAAGGCGTGAATATCCGTCATGATTAATCGGGTGTTCGGGTTGTGCTGTTGAATATAACTGCCAATCACCCCTGCGCCGCAGCCCACGTCCAacac > 1:589367/1‑240 (MQ=255) gtgtCAATGCCGTCATGAAAAGGCGGATTGGAAATGATCAGATCGAATTTGCCGTCAATA‑TGGGAAAACACGTCACTGGCAACCACCGTGCCTTGCAACCGATTTTCCTG‑TAAAGTGCGCTTTGCGGAAGCCAACGCCAAGGCGTGAATATCCGTCATGATTAATCGGGTGTTCGGGTTGTGCTGTTGAATATAACTGCCAATCACCCCTGCGCCGCAGCCCACGTCCAACACCTCGCCCGCGATGttttt > 1:274544/1‑251 (MQ=255) gtCAATGCCGTCATGAAAAGGCGGATTGGAAATGATCAGATCGAATTTGCCGTCAATA‑TGGGAAAACACGTCACTGGCAACCACCGTGCCTTGCAACCGATTTTCCTG‑TAAAGTGCGCTTTGCGGAAGCCAACGCCAAGGCGTGAATATCCGTCATg < 1:525035/157‑1 (MQ=255) gtCAATGCCGTCATGAAAAGGCGGATTGGAAATGATCAGATCGAATTTGCCGTCAATA‑TGGGAAAACACGTCACTGGCAACCACCGTGCCTTGCAACCGATTTTCCTG‑TAAAGTGCGCTTTGCGGAAGCCAACGCCAAGGCGTGAATATCCGTCATGAtt > 1:877830/1‑160 (MQ=255) gtcatGAAAAGGCGGATTGGAAATGATCAGATCGAATTTGCCGTCAATA‑TGGGAAAACACGTCACTGGCAACCACCGTGCCTTGCAACCGATTTTCCTG‑TAAAGTGCGCTTTGCGGAAGCCAACGCCAAGGCGTGAATATCCGTCATGATTAATCGGGTGTTCGGGTTGTGCTGTTGAATATAACTGCCAATCACCCCTGCGCCGCAGCCCACGTCCAACACCTCGCCCGCGATGTTTTTTAGCGTAGaaa > 1:108366/2‑251 (MQ=255) gtcatGAAAAGGCGGATTGGAAATGATCAGATCGAATTTGCCGTCAATA‑TGGGAAAACACGTCACTGGCAACCACCGTGCCTTGCAACCGATTTTCCTG‑TAAAGTGCGCTTTGCGGAAGCCAACGCCAAGGCGTGAATATCCGTCATGATTAATCGGGTGTTCGGGTTGTGCTGTTGAATATAACTGCCAATCACCCCTGCGCCGCAGCCCACGTCCAACACCTCGCCCGCGATGTTTTTTAGCGTAGaaa > 1:534545/2‑251 (MQ=255) catGAAAAGGCGGATTGGAAATGATCAGATCGAATTTGCCGTCAATA‑TGGGAAAACACGTCACTGGCAACCACCGTGCCTTGCAACCGATTTTCCTG‑TAAAGTGCGCTTTGCGGAAGCCAACGCCAAGGCGTGAATATCCGTCATGATTAATCGGGTGTTCGGGTTGTGCTGTTGAATATAACTGCCAATCACCCCTGCGCCGCAGCCCACGTCCAACACCTCGCCCGCGATGTTTTTTAGCGTAGAAAGc < 1:764399/251‑1 (MQ=255) tGAAAAGGCGGATTGGAAATGATCAGATCGAATTTGCCGTCAATA‑TGGGAAAACACGTCACTGGCAACCACCGTGCCTTGCAACCGATTTTCCTG‑TAAAGTGCGCTTTGCGGAAGCCAACGCCAAGGCGTGAATATCCGTCATGATTAATCGGGTGTTCGGGTTGTGCTGTTGAATATAACTGCCAATCACCCCTgcgc < 1:791792/199‑1 (MQ=255) aaaaggcggATTGGAAATGATCAGATCGAATTTGCCGTCAATA‑TGGGAAAACACGTCACTGGCAACCACCGTGCCTTGCAACCGATTTTCCTG‑TAAAGTGCGCTTTGCGGAAGCCAACGCCAAGGCGTGAATATCCGTCATGATTAATCGGGTGTTCGGGTTGTGCTGTTGAATATAACTGCCAATCACCCCTGCGCCGCAGCCCACGTCCAACACCTCGCCCGCGATGTTTTTTAGCGTAGAAAGCaat < 1:278914/246‑1 (MQ=255) gcggATTGGAAATGATCAGATCGAATTTGCCGTCAATA‑TGGGAAAACACGTCACTGGCAACCACCGTGCCTTGCAACCGATTTTCCTG‑TAAAGTGCGCTTTGCGGAAGCCAACGCCAAGGCGTGAATATCCGTCATGATTAATCGGGTGTTCGGGTTGTGCTGTTGAATATAACTGCCAATCACCCCTGCGCCGCAGCCCACGTCCAACACCTCGCCCGCGATGTTTTTTAGCGTAGAAAGCAATAAtgc < 1:142918/250‑1 (MQ=255) gattGGAAATGATCAGATCGAATTTGCCGTCAATA‑TGGGAAAACACGTCACTGGCAACCACCGTGCCTTGCAACCGATTTTCCTG‑TAAAGTGCGCTTTGCGGAAGCCAACGCCAAGGCGTGAATATCCGTCATGATTAATCGGGTGTTCGGGTTGTGCTGTTGAatat > 1:283830/3‑168 (MQ=255) gattGGAAATGATCAGATCGAATTTGCCGTCAATA‑TGGGAAAACACGTCACTGGCAACCACCGTGCCTTGCAACCGATTTTCCTG‑TAAAGTGCGCTTTGCGGAAGCCAACGCCAAGGCGTGAATATCCGTCATGATTAATCGGGTGTTCGGGTTGTGCTGTTGAATATAACTGCCAATCACCCCTGCGCCGCAGCCCACGTCCAACACCTCGCCCGCGATGTTTTTTAGCGTAGAAAGCAATAAtgccgtg > 1:93684/3‑251 (MQ=255) ggAAATGATCAGATCGAATTTGCCGTCAATA‑TGGGAAAACACGTCACTGGCAACCACCGTGCCTTGCAACCGATTTTCCTG‑TAAAGTGCGCTTTGCGGAAGCCAACGCCAAGGCGTGAATATCCGTCATGATTAATCGGGTGTTCGGGTTGTGCTGTTGAATATAACTGCCAATCACCCCTGCGCCGCAGCCCACGTCCAACACCTCGCCCGCGATGtttt > 1:436831/1‑221 (MQ=255) gAAATGATCAGATCGAATTTGCCGTCAATA‑TGGGAAAACACGTCACTGGCAACCACCGTGCCTTGCAACCGATTTTCCTG‑TAAAGTGCGCTTTGCGGAAGCCAACGCCAAGGCGTGAATATCCGTCATGATTAATCGGGTGTTCGGGTTGTGCTGTTGAATATAACTGCCAATCACCCCTGCGCCGCAGCCCACGTCCAACACCTCGCCCGCGATGTTTTTTAGCGTAGAAAGCAATAATGCCGTgccgc > 1:619016/1‑250 (MQ=255) tGATCAGATCGAATTTGCCGTCAATA‑TGGGAAAACACGTCACTGGCAACCACCGTGCCTTGCAACCGATTTTCCTG‑TAAAGTGCGCTTTGCGGAAGCCAACGCCAAGGCGTGAATATCCGTCATGATTAATCGGGTGTTCGGGTTGTGCTGTTGAATATAACTGCCAATCACCCCTGCGCCGCAGCCCACGTCCAACACCTCGCCCGCGATGTTTTTTAGCGTAGAAAGCAATAATGCCGTGCCGCCATcc < 1:706324/251‑1 (MQ=255) tGATCAGATCGAATTTGCCGTCAATA‑TGGGAAAACACGTCACTGGCAACCACCGTGCCTTGCAACCGATTTTCCTG‑TAAAGTGCGCTTTGCGGAAGCCAACGCCAAGGCGTGAATATCCGTCATGATTAATCGGGTGTTCGGGTTGTGCTGTTGAATATAACTGCCAATCACCCCTGCGCCGCAGCCCACGTCCAACACCTCGCCCGCGATGTTTTTTAGCGTAGAAAGCAATAATGCCGTGCCGCCATcc < 1:893288/251‑1 (MQ=255) aaTTTGCCGTCAATA‑TGGGAAAACACGTCACTGGCAACCACCGTGCCTTGCAACCGATTTTCCTG‑TAAAGTGCGCTTTGCGGAAGCCAACGCCAAGGCGTGAATATCCGTCATGATTAATCGGGTGTTCGGGTTGTGCTGTTGAATATAACTGCCAATCACCCCTGCGCCGCAGCCCACGTCCAACACCTCGCCCGCGATGTTTTTTAGCGTAGAAAGCAATAATGCCGTGCCGCCATCCAGCTcggcgg < 1:38442/250‑1 (MQ=255) cGTCAATA‑TGGGAAAACACGTCACTGGCAACCACCGTGCCTTGCAACCGATTTTCCTG‑TAAAGTGCGCTTTGCGGAAGCCAACGCCAAGGCGTGAATATCCGTCATGATTAATCGGGTGTTCGGGTTGTGCTGTTg > 1:848212/1‑136 (MQ=255) tCAATA‑TGGGAAAACACGTCACTGGCAACCACCGTGCCTTGCAACCGATTTTCCTG‑TAAAGTGCGCTTTGCGGAAGCCAACGCCAAGGCGTGAATATCCGTCATGATTAATCGGGTGTTCGGGTTGTGCTGTTGAATATAACTGCCAATCACCCCTGCGCCGCAGCCCACGTCCAACACCTCGCCCGCGATGTTTTTTAGCGTAGAAAGCAATAATGCCGTGCCGCCATCCAGCTCGGCGGCGCTGAATAc < 1:663360/251‑1 (MQ=255) cAATA‑TGGGAAAACACGTCACTGGCAACCACCGTGCCTTGCAACCGATTTTCCTG‑TAAAGTGCGCTTTGCGGAAGCCAACGCCAAGGCGTGAATATCCGTCATGATTAATCGGGTGTTCGGGTTGTGCTGTTGAATATAACTGCCAATCACCCCTGCGCCGCAGCCCACGTCCAACACCTCGCCCGCGATGTTTTTTAGCGTAGAAAGCAATAATGCCGTGCCGCCATCCAGCTCGGCGGCGCTGAATACg > 1:843908/1‑251 (MQ=255) ggAAAACACGTCACTGGCAACCACCGTGCCTTGCAACCGATTTTCCTG‑TAAAGTGCGCTTTGCGGAAGCCAACGCCAAGGCGTGAATATCCGTCATGATTAATCGGGTGTTCGGGTTGTGCTGTTGAATATAACTGCCAATCACCCCTGCGCCGCAGCCCACGTCCAACACCTCGCCCGCGATGTTTTTTAGCGTAGAAAGCAATAATGCCGTGCCGCCATCCAGCTCGGCGGCGCTGAATAc > 1:360564/1‑243 (MQ=255) | CACCGAATAAACCTTAAACTTGTTATTTCTCGCCAGGACGTCATGACTGCCGAAATGTTGATTCAGCCAGTCGGCGTAAGGCAGAAACGCATTTGCCACGATGCGCAGCTCACCGCCCGGTTTCAAATGCCATTTTGCCTGTTTTATCAGTTCGTTAACGGCGGTGTAAGCGGTGTCAATGCCATCATGAAACGGCGGGTTGGAAATGATCAGATCAAATTTGCCGTCAATA‑TGGGAAAACACATCACTGGCAACCACTGTTCCCTGTAGCCGATTTTCCTG‑TAAAGTGCGCTTTGCGGAAGCCAACGCCAAGGCATGAATATCTGTCATGATTAATCGGGTGTTCGGGTTGTGCCGTTGAATATAACTGCCGATCACTCCCGCGCCGCAGCCCACGTCCAACACCCCGCCTGCGATGTTTTTTAGCGTAGAAAGCAATAATGCCGTGCCGCCATCCAGTTCGGCGGCGCTGAATACG > 1/1986316‑1986793 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |