Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,986,532	A→G	F260F (TTT→TTC)	unknown ←	16S rRNA (guanine(1207)‑N(2))‑methyltransferase (EC 2.1.1.172)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,986,532	0	A	G	100.0%	217.5 / NA	77	F260F (TTT→TTC)	unknown	16S rRNA (guanine(1207)‑N(2))‑methyltransferase (EC 2.1.1.172)
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (32/45);  total (32/45)

CACTTAACTCATCCGTGCACCGAATAAACCTTAAACTTGTTATTTCTCGCCAGGACGTCATGACTGCCGAAATGTTGATTCAGCCAGTCGGCGTAAGGCAGAAACGCATTTGCCACGATGCGCAGCTCACCGCCCGGTTTCAAATGCCATTTTGCCTGTTTTATCAGTTCGTTAACGGCGGTGTAAGCGGTGTCAATGCCATCATGAAACGGCGGGTTGGAAATGATCAGATCAAATTTGCCGTCAATA‑TGGGAAAACACATCACTGGCAACCACTGTTCCCTGTAGCCGATTTTCCTG‑TAAAGTGCGCTTTGCGGAAGCCAACGCCAAGGCATGAATATCTGTCATGATTAATCGGGTGTTCGGGTTGTGCCGTTGAATATAACTGCCGATCACTCCCGCGCCGCAGCCCACGTCCAACACCCCGCCTGCGATGTTTTTTAGCGTAGAAAGCAATAATGCCGTGCCGCCATCC  >  1/1986299‑1986772                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                   cacTTAACTCATCTGCGCACCGAATATACCTTAAACTTGTTATTTCTCGCCAGGACGTCATGACTGCCGAAATGTTGATTCAGCCAGTCGGCGTAAGGCAGAAACGCATTTGCCACGATGCGCAGCTCACCGCCCGGTTTCAAATGCCATTTTGCCTGTTTTATCAGTTCGTTAACGGCGGTGTAAGCGGGGGCAATGCCGGCCTGGAAAGGGGGGTTGGGAATGATTAGATCGAATTTggcggcaaaatg                                                                                                                                                                                                                                   >  1:675427/1‑240 (MQ=255) cacTTAACTCATCTGCGCACCGAATATACCTTAAACTTGTTATTTCTCGCCAGCACGTCATGACTGCCGAAATGTTGATTCAGCCAGTCGGCGTAAGGCAGAAACGCATTTGCCACGATGCGCAGCTCACCGCCCGGTTTCAAATGCCATTTTGCCTGTTTTATCAGTTCGTTAACGGCGGTGTAAGAGGGGGCCATGCCCTCCTGAAAAAGCGGATTTGAAATGATTAGATCGAATTTTCCGTCCATA‑Tg                                                                                                                                                                                                                                  >  1:623715/1‑251 (MQ=255)   cTTAACTCATCTGCGCACCGAATATACCTTAAACTTGTTATTTCTCGCCAGGACGTCATGACTGCCGAAATGTTGATTCAGCCAGTCGGCGTAAGGCAGAAACGCATTTGCCACGATGCGCAGCTCACCGCCCGGTTTCAAATGCCATTTTGCCTGTTTTATCAGTTCGTTAACGGCGGTGTAAGCGGTGTCAATGCCGTCATGAAAAGGCGGATTGGAAATGATCAGATCGAATTTGCCGTCAATA‑Tggg                                                                                                                                                                                                                                <  1:66558/251‑1 (MQ=255)   cTTAACTCATCTGCGCACCGAATATACCTTAAACTTGTTATTTCTCGCCAGGACGTCATGACTGCCGAAATGTTGATTCAGCCAGTCGGCGTAAGGCAGAAACGCATTTGCCACGATGCGCAGCTCACCGCCCGGTTTCAAATGCCATTTTGCCTGTTTTATCAGTTCGTTAACGGCGGTGTAAGCGGTGTCAATGCCGTCATGAAAAGGCGGATTGGAAATGATCAGATCGAATTTGCCGTCAATA‑Tggg                                                                                                                                                                                                                                <  1:829198/251‑1 (MQ=255)   cTTAACTCATCTGCGCACCGAATATACCTTAAACTTGTTATTTCTCGCCAGGACGTCATGACTGCCGAAATGTTGATTCAGCCAGTCGGCGTAAGGCAGAAACGCATTTGCCACGATGCGCAGCTCACCGCCCGGTTTCAAATGCCATTTTGCCTGTTTTATCAGTTCGTTAACGGCGGTGTAAGCGGGGTCAATGCCCTCACTAAAAGGCGGATTGGAAATGATCAGATCGAATTTTCCGTCAATA‑Tggg                                                                                                                                                                                                                                >  1:702141/1‑251 (MQ=255)     tAACTCATCTGCGCACCGAATATACCTTAAACTTGTTATTTCTCGCCAGGACGTCATGACTGCCGAAATGTTGATTCAGCCAGTCGGCGTAAGGCAGAAACGCATTTGCCACGATGCGCAGCTCACCGCCCGGTTTCAAATGCCATTTTGCCTGTTTTATCAGTTCGTTAACGGCGGTGTAAGCGGTGTCAATGCCGTCATGAAAAGGCGGATTGGAAATGATCAGATCGAATTTGCCGTCAATA‑TGGGaa                                                                                                                                                                                                                              <  1:729665/251‑1 (MQ=255)     tAACTCATCTGCGCACCGAATATACCTTAAACTTGTTATTTCTCGCCAGGACGTCATGACTGCCGAAATGTTGATTCAGCCAGTCGGCGTAAGGCAGAAACGCATTTGCCACGATGCGCAGCTCACCGCCCGGTTTCAAATGCCATTTTGCCTGTTTTATCAGTTCGTTAACGGCGGTGTAAGCGGTGTCAATGCCGTCATGAAAAGGCGGATTGGAAATGATCAGATCGAATTTGCCGTCAATA‑TGGGaa                                                                                                                                                                                                                              <  1:800969/251‑1 (MQ=255)        cTCATCTGCGCACCGAATATACCTTAAACTTGTTATTTCTCGCCAGGACGTCATGACTGCCGAAATGTTGATTCAGCCAGTCGGCGTAAGGCAGAAACGCATTTGCCACGATGCGCAGCTCACCGCCCGGTTTCAAATGCCATTTTGCCTGTTTTATCAGTTCGTTAACGGCGGTGTAAGCGGTGTCAATGCCGTCATGAAAAGGCGGATTGGAAATGATCAGATCGAATTTGCCGTCAATA‑TGGGAAAac                                                                                                                                                                                                                           <  1:739006/251‑1 (MQ=255)            tctgcgCACCGAATATACCTTAAACTTGTTATTTCTCGCCAGGACGTCATGACTGCCGAAATGTTGATTCAGCCAGTCGGCGTAAGGCAGAAACGCATTTGCCACGATGCGCAGCTCACCGCCCGGTTTCAAATGCCATTTTGCCTGTTTTATCAGTTCGTTAACGGCGGTGGAAGCGGGGTCAATGGCGTCATGAAAAAGCGGGTTGGGAATGATCAGATCGAATTTGGCGGCAATA‑TGGGaaaaaacgg                                                                                                                                                                                                                       >  1:318/6‑246 (MQ=255)                  cACCGAATATACCTTAAACTTGTTATTTCTCGCCAGGACGTCATGACTGCCGAAATGTTGATTCAGCCAGTCGGCGTAAGGCAGAAACGCATTTGCCACGATGCGCAGCTCACCGCCCGGTTTCAAATGCCATTTTGCCTGTTTTATCAGTTCGTTAACGGCGGTGTAAGCGGTGTCAATGCCGTCATGAAAAGGCGGATTGGAAATGATCAGATCGAATTTGCCGTCAATA‑TGGGAAAACACGTCACTgg                                                                                                                                                                                                                 <  1:475818/251‑1 (MQ=255)                  cACCGAATATACCTTAAACTTGTTATTTCTCGCCAGGACGTCATGACTGCCGAAATGTTGATTCAGCCAGTCGGCGTAAGGCAGAAACGCATTTGCCACGATGCGCAGCTCACCGCCCGGTTTCAAATGCCATTTTGCCTGTTTTATCAGTTCGTTAACGGCGGTGTAAGCGGTGTCAATGCCGTCATGAAAAGGCGGATTGGAAATGATCAGATCGAATTTGCCGTCAATA‑TGGGAAAACACGTCACTgg                                                                                                                                                                                                                 <  1:740646/251‑1 (MQ=255)                    ccGAATATACCTTAAACTTGTTATTTCTCGCCAGGACGTCATGACTGCCGAAATGTTGATTCAGCCAGTCGGCGTAAGGCAGAAACGCATTTGCCACGATGCGCAGCTCACCGCCCGGTTTCAAATGCCATTTTGCCTGTTTTATCAGTTCGTTAACGGCGGTGTAAGCGGTGTCAATGCCGTCATGAAAAGGCGGATTGGAAATGATCAGATCGAATTTGCCGTCAATA‑Tggg                                                                                                                                                                                                                                <  1:782848/234‑1 (MQ=255)                    ccGAATATACCTTAAACTTGTTATTTCTCGCCAGGACGTCATGACTGCCGAAATGTTGATTCAGCCAGTCGGCGTAAGGCAGAAACGCATTTGCCACGATGCGCAGCTCACCGCCCGGTTTCAAATGCCATTTTGCCTGTTTTATCAGTTCGTTAACGGCGGTGTAAGCGGGGTCAATGCCGTCATGAAAAGGCGGGTTGGGAATGATCAGATCGAAATTGCCGTCAATA‑TGGGGAAAAACGTCACTGGCa                                                                                                                                                                                                               >  1:327429/1‑251 (MQ=255)                    ccGAATATACCTTAAACTTGTTATTTCTCGCCAGGACGTCATGACTGCCGAAATGTTGATTCAGCCAGTCGGCGTAAGGCAGAAACGCATTTGCCACGATGCGCAGCTCACCGCCCGGTTTCAAATGCCATTTTGCCTGTTTTATCAGTTCGTTAACGGCGGTGGAAGCGGTGTCCATGCCGTCCTTAAAAAGCGGGTTGGAAATTATCAGATCGAATTTTCCGTCCATA‑TTGGAAAACACACCACTGGCa                                                                                                                                                                                                               >  1:887063/1‑251 (MQ=255)                         tataCCTTAAACTTGTTATTTCTCGCCAGGACGTCATGACTGCCGAAATGTTGATTCAGCCAGTCGGCGTAAGGCAGAAACGCATTTGCCACGATGCGCAGCTCACCGCCCGGTTTCAAATGCCATTTTGCCTGTTTTATCAGTTCGTTAACGGCGGTGTAAGCGGTGTCAATGCCGTCATGAAAAGGCGGATTGGAAATGATCAGATCGAATTTGCCGTCAATA‑TGGGAAAACACGTCACTGGCAACCAc                                                                                                                                                                                                          <  1:235068/248‑1 (MQ=255)                            aCCTTAAACTTGTTATTTCTCGCCAGGACGTCATGACTGCCGAAATGTTGATTCAGCCAGTCGGCGTAAGGCAGAAACGCATTTGCCACGATGCGCAGCTCACCGCCCGGTTTCAAATGCCATTTTGCCTGTTTTATCAGTTCGTTAACGGCGGTGTAAGCGGGGTCAATGCCGTCCTGGAAAGGGGGGTTGGGAATGATCAGATCGAATTTGCCGGCAATATTGGGAAAA‑AAGTCACTGGGAACCAccgg                                                                                                                                                                                                       >  1:224597/1‑248 (MQ=255)                              cTTAAACTTGTTATTTCTCGCCAGGACGTCATGACTGCCGAAATGTTGATTCAGCCAGTCGGCGTAAGGCAGAAACGCATTTGCCACGATGCGCAGCTCACCGCCCGGTTTCAAATGCCATTTTGCCTGTTTTATCAGTTCGTTAACGGCGGTGTAAGCGGTGTCAATGCCGTCATGAAAAGGCGGATTGGAAATGATCAGATCGAATTTGCCGTCAATA‑TGGGAAAACACGTCACTGGCAACCAccgtgc                                                                                                                                                                                                     <  1:836938/251‑6 (MQ=255)                                          aTTTCTCGCCAGGACGTCATGACTGCCGAAATGTTGATTCAGCCAGTCGGCGTAAGGCAGAAACGCATTTGCCACGATGCGTAGCTCACCGCCCGGTTTCAAATGCCATTTTGCCTGTTTTATCAGTTCGTTAACGGCGGTGTAAGCGGTGTCAATGCCGTCATGAAAAGGCGGATTGGAAATGATCAGATCGAATTTGCCGTCAATA‑TGGGAAAACACGTCACTGGCAACCAccgtgccttgcaaccgat                                                                                                                                                                                         <  1:448429/251‑18 (MQ=255)                                                cGCCAGGACGTCATGACTGCCGAAATGTTGATTCAGCCAGTCGGCGTAAGGCAGAAACGCATTTGCCACGATGCGCAGCTCACCGCCCGGTTTCAAATGCCATTTTGCCTGTTTTATCAGTTCGTTAACGGCGGTGTAAGCGGTGTCAATGCCGTCATGAAAAGGCGGATTGGAAATGATCAGATCGAATTTGCCGTCAATA‑TGGGAAAACACGTCACTGGCAACCAc                                                                                                                                                                                                          <  1:630124/228‑1 (MQ=255)                                                       aCGTCATGACTGCCGAAATGTTGATTCAGCCAGTCGGCGTAAGGCAGAAACGCATTTGCCACGATGCGCAGCTCACCGCCCGGTTTCAAATGCCATTTTGCCTGTTTTATCAGTTCGTTAACGGCGGTGTAAGCGGTGTCAATGCCGTCATGAAAAGGCGGATTGGAAATGATCAGATCGAATTTGCCGTCAATA‑TGGGAAAACACGTCACTGGCAACCACCGTGCCTTGCAACCGATTTTCCTG‑TAAAg                                                                                                                                                                            <  1:386539/250‑1 (MQ=255)                                                       aCGTCATGACTGCCGAAATGTTGATTCAGCCAGTCGGCGTAAGGCAGAAACGCATTTGCCACGATGCGCAGCTCACCGCCCGGTTTCAAATGCCATTTTGCCTGTTTTATCAGTTCGTTAACGGCGGTGTAAGCGGTGTCAATGCCGTCATGAAAAGGCGGATTGGAAATGATCAGATCGAATTTGCCGTCAATA‑TGGGAAAACACGTCACTGGCAACCACCGTGCCTTGCAACCGATTTTCCTG‑TAAAg                                                                                                                                                                            <  1:506731/250‑1 (MQ=255)                                                        cGTCATGACTGCCGAAATGTTGATTCAGCCAGTCGGCGTAAGGCAGAAACGCATTTGCCACGATGCGCAGCTCACCGCCCGGTTTCAAATGCCATTTTGCCTGTTTTATCAGTTCGTTAACGGCGGTGTAAGCGGTGTCAATGCCGTCATGAAAAGGCGGATTGGAAATGATCAGATCGAATTTGCCGTCAATA‑Tggg                                                                                                                                                                                                                                <  1:121830/198‑1 (MQ=255)                                                                cTGCCGAAATGTTGATTCAGCCAGTCGGCGTAAGGCAGAAACGCATTTGCCACGATGCGCAGCTCACCGCCCGGTTTCAAATGCCATTTTGCCTGTTTTATCAGTTCGTTAACGGCGGTGTAAGCGGTGTCAATGCCGTCATGAAAAGGCGGATTGGAAATGATCAGATCGAATTTGCCGTCAATA‑TGGGaa                                                                                                                                                                                                                              <  1:621521/192‑1 (MQ=255)                                                                         tGTTGATTCAGCCAGTCGGCGTAAGGCAGAAACGCATTTGCCACGATGCGCAGCTCACCGCCCGGTTTCAAATGCCATTTTGCCTGTTTTATCAGTTCGTTAACGGCGGTGTAAGCGGTGTCAATGCCGTCATGAAAAGGCGGATTGGAAATGATCAGATCGAATTTGCCGTCAATA‑Tggg                                                                                                                                                                                                                                <  1:334201/181‑1 (MQ=255)                                                                                               aaGGCAGAAACGCATTTGCCACGATGCGCAGCTCACCGCCCGGTTTCAAATGCCATTTTGCCTGTTTTATCAGTTCGTTAACGGCGGTGTAAGCGGTGTCAATGCCGTCATGAAAAGGCGGATTGGAAATGATCAGATCGAATTTGCCGTCAATA‑TGGGAAAACACGTCACTGGCAACCACCGTGCCTTGCAACCGATTTTCCTG‑TAAAGTGCGCTTTGCGGAAGCCAACGCCAAGGCGTGAATATCCGTc                                                                                                                                   <  1:626801/251‑1 (MQ=255)                                                                                                     gAAACGCATTTGCCACGATGCGCAGCTCACCGCCCGGTTTCAAATGCCATTTTGCCTGTTTTATCAGTTCGTTAACGGCGGTGTAAGCGGTGTCAATGCCGTCATGAAAAGGCGGATTGGAAATGATCAGATCGAATTTGCCGTCAATA‑TGGGAAAACACGTCACTGGCAACCACCGTGCCTTGCAACCGATTTTCCTG‑TAAAGTGCGCTTTGCGGAAGCCAACGCCAAGGCGTGAat                                                                                                                                          <  1:601032/238‑1 (MQ=255)                                                                                                     gAAACGCATTTGCCACGATGCGCAGCTCACCGCCCGGTTTCAAATGCCATTTTGCCTGTTTTATCAGTTCGTTAACGGCGGTGTAAGCGGGGTCAATGCCGTCATGAAAAAGCGGATTGGGAATGATCAGATCGAATTTTCCGTCAATA‑TGGGAAAAAACGTCACTGGCAACCACCGGGCCTTGCAACCGATTTTTCTG‑TAAAg                                                                                                                                                                            >  1:77823/1‑204 (MQ=255)                                                                                                      aaaCGCATTTGCCACGATGCGCAGCTCACCGCCCGGTTTCAAATGCCATTTTGCCTGTTTTATCAGTTCGTTAACGGCGGTGTAAGCGGTGTCAATGCCGTCATGAAAAGGCGGATTGGAAATGATCAGATCGAATTTGCCGTCAATA‑TGGGAAAACACGTCACTGGCAACCACCGTGCCTTGCAACCGATTTTCCTG‑TAAAGTGCGCTTTGCGGAAGCCAACGCCAAGGCGTGAATATCCGTCATGa                                                                                                                               <  1:806713/248‑1 (MQ=255)                                                                                                      aaaCGCATTTGCCACGATGCGCAGCTCACCGCCCGGTTTCAAATGCCATTTTGCCTGTTTTATCAGTTCGTTAACGGCGGTGTAAGCGGTGTCAATGCCGTCATGAAAAGGCGGATTGGAAATGATCAGATCGAATTTGCCGTCAATA‑TGGGAAAACACGTCACTGGCAACCACCGTGCCTTGCAACCGATTTTCCTG‑TAAAGTGCGCTTTGCGGAAGCCAACGCCAAGGCGTGAATATCCGTCATGAtt                                                                                                                             <  1:762075/250‑1 (MQ=255)                                                                                                           cATTTGCCACGATGCGCAGCTCACCGCCCGGTTTCAAATGCCATTTTGCCTGTTTTATCAGTTCGTTAACGGCGGTGTAAGCGGTGTCAATGCCGTCATGAAAAGGCGGATTGGAAATGATCAGATCGAATTTGCCGTCAATA‑Tggg                                                                                                                                                                                                                                <  1:803955/147‑1 (MQ=255)                                                                                                                gCCACGATGCGCAGCTCACCGCCCGGTTTCAAATGCCATTTTGCCTGTTTTATCAGTTCGTTAACGGCGGTGTAAGCGGGGTCAATGCCGTCATGAAAAGGCGGGTTGGGAATGATCAGATCGAATTTGCCGTCAATA‑TGGGGAAACAAGTCACTGGCAACCACCCTGCCTTGCAACCGATTTTTCTG‑TAAAATGCGCTTTGCGGAAGCCAACGCCAAGGCGGGAATATCCGTCATGATTAATCGGGGGtt                                                                                                                  >  1:597229/1‑251 (MQ=255)                                                                                                                 ccACGATGCGCAGCTCACCGCCCGGTTTCAAATGCCATTTTGCCTGTTTTATCAGTTCGTTAACGGCGGTGTAAGCGGTGTCAATGCCGTCATGAAAAGGCGGATTGGAAATGATCAGATCGAATTTGCCGTCAATA‑TGGGAAAACACGTCACTGGCAACCAc                                                                                                                                                                                                          <  1:654530/163‑1 (MQ=255)                                                                                                                    cGATGCGCAGCTCACCGCCCGGTTTCAAATGCCATTTTGCCTGTTTTATCAGTTCGTTAACGGCGGTGTAAGCGGTGTCAATGCCGTCATGAAAAGGCGGATTGGAAATGATCAGATCGAATTTGCCGTCAATA‑TGGGAAAACACGTCACTGGCAACCACCGTGCCTTGCAACCGATTTTCCTG‑TAAAGTGCGCTTTGCGGAAGCCAACGCCAAGGCGTGAATATCCGTCATGATTAATCGGGTGTTCggg                                                                                                              <  1:25843/251‑1 (MQ=255)                                                                                                                      aTGCGCAGCTCACCGCCCGGTTTCAAATGCCATTTTGCCTGTTTTATCAGTTCGTTAACGGCGGTGTAAGCGGTGTCAATGCCGTCATGAAAAGGCGGATTGGAAATGATCAGATCGAATTTGCCGTCAATA‑TGGGAAAACACGTCACTGGCAACCACCGTGCCTTGCAACCGATTTTCCTG‑TAAAGTGCGCTTTGCGGAAGCCAACGCCAAGGCGTGAATATCCGTCATGATTAATCGGGTGTTCGGGt                                                                                                             <  1:2235/250‑1 (MQ=255)                                                                                                                      aTGCGCAGCTCACCGCCCGGTTTCAAATGCCATTTTGCCTGTTTTATCAGTTCGTTAACGGCGGTGGAAGCGGGGTCAAAGGCGTCATGAAAAGGGGGGATGGGAAAGATTAAAACGAATTTGGCGTTAATA‑TGGGGAAACACGGTACCGGGAACCACCCGGCCCTGCAGCCGATTTTTCTGTTAAAG‑GCGCCTTGCGGGAGCCAACGCCAAGGCGGGGAAATTCGTCATGATTAATCggggggttggggt                                                                                                            >  1:456001/1‑241 (MQ=255)                                                                                                                        gcgcAGCTCACCGCCCGGTTTCAAATGCCATTTTGCCTGTTTTATCAGTTCGTTAACGGCGGTGTAAGCGGTGTCAATGCCGTCATGAAAAAGCGGGTTGGAAATGATCAGATCGAATTTGCCGTCAATA‑TGGGAAAAAACGTCACTGGGAACCACCGTGCCTTGCAACCGCTTTTCCTG‑TAAAGTGCGCTTTGCGGGAGCCAACGCCAAGGCGTGGATATCCGTCCTGATTAATctgggggtcgggg                                                                                                             >  1:652145/1‑236 (MQ=255)                                                                                                                        gcgcAGCTCACCGCCCGGTTTCAAATGCCATTTTGCCTGTTTTATCAGTTCGTTAACGGCGGTGTAAGCGGGGTCAATGCCGTCATGAAAAGGCGGATTGGGAATGATCAGATTGAATTTTCCGGCAATA‑TGGGGAAAAACGGCAATGGCAACCACCCTGCCTTGCAACCGATTTTTCTG‑TAAAGTGCGCTTTGCGGGAGCCAACGCCCAGGGGGGAATATTCCTCATGATTAATCGGGGGTTCggggtgg                                                                                                          >  1:56099/1‑247 (MQ=255)                                                                                                                           cAGCTCACCGCCCGGTTTCAAATGCCATTTTGCCTGTTTTATCAGTTCGTTAACGGCGGTGTAAGCGGTGTCAATGCCGTCATGAAAAGGCGGATTGGAAATGATCAGATCGAATTTGCCGTCAATA‑TGGGAAAACACGTCACTGGCAACCACCGTGCCTTGCAACCGATTTTCCTG‑TAAAGTGCGCTTTGCGGAAGCCAACGCCAAGGCGTGAATATCCGTCATGATTAATCGGGTGTTCGGGTTGTGc                                                                                                        <  1:116878/250‑1 (MQ=255)                                                                                                                           cAGCTCACCGCCCGGTTTCAAATGCCATTTTGCCTGTTTTATCAGTTCGTTAACGGCGGTGTAAGCGGTGTCAATGCCGTCATGAAAAGGCGGATTGGAAATGATCAGATCGAATTTGCCGTCAATA‑TGGGAAAACACGTCACTGGCAACCACCGTGCCTTGCAACCGATTTTCCTG‑TAAAGTGCGCTTTGCGGAAGCCAACGCCAAGGCGTGAATATCCGTCATGATTAATCGGGTGTTCGGGTTGTGc                                                                                                        <  1:725967/250‑1 (MQ=255)                                                                                                                             gCTCACCGCCCGGTTTCAAATGCCATTTTGCCTGTTTTATCAGTTCGTTAACGGCGGTGTAAACGGGGGCAATGCCGTCCTTAAAAAGCGGGTTGGGAAAGATCAGATCGAATTTGCCGGCCATA‑TGGGGAAAAACGTCACTGGCCACCCCCGGGCCTTTCAACCGATTTTTCTG‑TAAAGAGCGCTTTGCGGGAGCCCACGCCAAGGCGTGAATATCCGTCCTGATTAATCGGGGGTTCGGGGTGTGctgg                                                                                                     >  1:889829/1‑248 (MQ=255)                                                                                                                                     cccGGTTTCAAATGCCATTTTGCCTGTTTTATCAGTTCGTTAACGGCGGTGTAAGCGGTGTCAATGCCGTCATGAAAAGGCGGATTGGAAATGATCAGATCGAATTTGCCGTCAATA‑TGGGAAAACACGTCACTGGCAACCACCGTGCCTTGCAACCGATTTTCCTG‑TAAAGTGCGCTTTGCGGAAGCCAACGCCAAGGCGTGAATATCCGTCATGATTAATCGGGTGTTCGGGTTGTGCTGTTGAatat                                                                                              <  1:294368/250‑1 (MQ=255)                                                                                                                                       cGGTTTCAAATGCCATTTTGCCTGTTTTATCAGTTCGTTAACGGCGGTGTAAGCGGTGTCAATGCCGTCATGAAAAGGCGGATTGGAAATGATCAGATCGAATTTGCCGTCAATA‑TGGGAAAACACGTCACTGGCAACCACCGTGCCTTGCAACCGATTTTCCTG‑TAAAGTGCGCTTTGCGGAAGCCAACGCCAAGGCGTGAATATCCGTCATGATTAATCGGGTGTTCGGGTTGTGCTGTTGAATATAAc                                                                                           <  1:763838/251‑1 (MQ=255)                                                                                                                                           ttCAAATGCCATTTTGCCTGTTTTATCAGTTCGTTAACGGCGGTGTAAGCGGGGTCAATGCCGTCCTGAAAAGGCGGGTTGGGAATGATCAGATCGAATTTTCCGTCAATA‑TGGGGAAAAACGTCACTGGCAACCACCCTGCCTTGCAAACGATTTTCCTG‑TAAAATGCGCTTTTCGGAAGACAACGCCAAGGCGGGAATATCCGTCATGATTAAACGGGGGTTCGGGGTGTGCTGTTGAATATAAATGc                                                                                        >  1:830181/1‑250 (MQ=255)                                                                                                                                             cAAATGCCATTTTGCCTGTTTTATCAGTTCGTTAACGGCGGTGTAAGCGGGGTCAAAGCCGTCATGAAAAGGGGGATTGGGAATGAAAAGATCGAATTTGCCGTCAATA‑TGGGAAAACAAGACACTGGCAACCACCGTGCCCTGCAAACGATTTTCCTG‑TAAAGTGCGCTTTTTGGAAGCCAACGCCAAGGCGGGGATATCCTTCATGGTTAAATGGGTGGTCGGGTTGTGCTGTTGTATATAAATGccaa                                                                                     >  1:220091/1‑249 (MQ=255)                                                                                                                                                aTGCCATTTTGCCTGTTTTATCAGTTCGTTAACGGCGGTGTAAGCGGTGTCAATGCCGTCATGAAAAGGCGGATTGGAAATGATCAGATCGAATTTGCCGTCAATA‑TGGGAAAACACGTCACTGGCAACCACCGTGCCTTGCAACCGATTTTCCTG‑TAAAGTGCGCTTTGCGGAAGCCAACGCCAAGGCGTGAATATCCGTCATGATTAATCggg                                                                                                                      <  1:673398/215‑1 (MQ=255)                                                                                                                                                   ccATTTTGCCTGTTTTATCAGTTCGTTAACGGCGGTGTAAGCGGTGTCAATGCCGTCATGAAAAGGCGGATTGGAAATGATCAGATCGAATTTGCCGTCAATA‑TGGGAAAACACGTCACTGGCAACCAc                                                                                                                                                                                                          <  1:631068/129‑1 (MQ=255)                                                                                                                                                    cATTTTGCCTGTTTTATCAGTTCGTTAACGGCGGTGTAAGCGGTGTCAATGCCGTCATGAAAAGGCGGATTGGAAATGATCAGATCGAATTTGCCGTCAATA‑TGGGAAAACACGTCACTGGCAACCACCGTGCCTTGCAACCGATTTTCCTG‑TAAAGTGCGCTTTGCGGAAGCCAACGCCAAGGCGTGAATATCCGTCATGATTAATCGGGTGTTCGGGTTGTGCTGTTGAATATAACTGCCAATCAcccc                                                                              <  1:549221/251‑4 (MQ=255)                                                                                                                                                    cATTTTGCCTGTTTTATCAGTTCGTTAACGGCGGTGTAAGCGGTGTCAATGCCGTCATGAAAAGGCGGATTGGAAATGATCAGATCGAATTTGCCGTCAATA‑TGGGAAAACACGTCACTGGCAACCACCGTGCCTTGCAACCGATTTTCCTG‑TAAAGTGCGCTTTGCGGAAGCCAACGCCAAGGCGTGAATATCCGTCATGATTAATCGGGTGTTCGGGTTGTGCTGTTGAATATAACTGCCAATCAcccc                                                                              <  1:850484/251‑4 (MQ=255)                                                                                                                                                    cATTTTGCCTGTTTTATCAGTTCGTTAACGGCGGTGTAAGCGGGGTCAATGTCGTCCTGAAAAGGCGGGTTGGGAAAGATCAGATCGAATTTTTCGTCAATA‑TGGGAAAAAACGTCACTGGCAACCACCGCGCCTTGCAAACGATTTTTCTG‑TTAAGTGCGCTTTTCGGGAGCCAAAGCCAAGGCGGGAATATCCGTCATGATTAATCGGGGGTTCGGGGTGTGCTGTTGAATATAAATGCCAATCAccc                                                                               >  1:479260/1‑248 (MQ=255)                                                                                                                                                              gTTTTATCAGTTCGTTAACGGCGGTGTAAGCGGGGTCAATGCCGTCATGAAAAGGCGGGTTGGGAAAGATCAGATCGAATTTGCCGTCAATA‑TGGGGAAACACGTCCCTGGCAAACACCCTGCCTTGCAACCGATTTTTCTG‑TAAAGGGCGCTTTGCGGAAGCCAACGCCAAGGCGGGAATATCCGTCCTGATTAATCGGGTGTTCGGGGTGTGCTGTTGGatat                                                                                              >  1:316686/1‑225 (MQ=255)                                                                                                                                                              gTTTTATCAGTTCGTTAACGGCGGTGTAAGCGGGGTCAAAGCCGTCATGAAAAAGCGGGTTGGAAAAGAACAAATCGAATTTGCCGTCAAAA‑AGGGGAAAAACGTCACTGGCAAACACCGTGCCCTGCAACCGATTTTTCTG‑TAAAGAGCGCTTTGCGGAAGCCAACGCCCAGGCGTGAATATCCGGCATGATTAATCGGGGGTTCGGGGTGGGCTGTTGAATATAAATGCCAATCAACCCTGCGCCGCAg                                                                    >  1:482006/1‑251 (MQ=255)                                                                                                                                                                 ttATCAGTTCGTTAACGGCGGTGTAAGCGGTGTCAATGCCGTCATGAAAAGGCGGATTGGAAATGATCAGATCGAATTTGCCGTCAATA‑TGGGAAAACACGTCACTGGCAACCACCGTGCCTTGCAACCGATTTTCCTG‑TAAAGTGCGCTTTGCGGAAGCCAACGCCAAGGCGTGAATATCCGTCATGATTAATCGGGTGTTCGGGTTGTGCTGTTGAATATAACTGCCAATCACCCCTGCGCCGCAGccc                                                                 <  1:358242/251‑1 (MQ=255)                                                                                                                                                                  tATCAGTTCGTTAACGGCGGTGTAAGCGGGGTCAATGCCGTCCTGAAAAAGCGGGTTGGGAATGATCAGATCGAATTTTCCGTCAATA‑TTGGGAAACACGTCACTGGCAACCACCCTGCCCTGCAACCGATTTTTCTT‑TAAAATGCGCTTTTCGGGAGCCAACGCCCAGGCGGGGATATCCCTCCTGATTAATCTGGGGTTCGGGGTTTGCTGTTGAATATAAATGCCCATCACCCCCGcgcccccgccc                                                                 >  1:140971/1‑243 (MQ=255)                                                                                                                                                                       gTTCGTTAACGGCGGTGTAAGCGGGGTCAATGCCGTCATGAAAAAGCGGATTGGGAATGATCAGATCGAATTTGCCGTCCATA‑TGGGGAAACACGTCACTGGCAACCACCCTGCCTTGCAACCGATTTTCCTG‑TAAAGTGCGCTTTGCGGGAGCCAACGCCCAGGCGTGAATATCCGTCATGATTAATCGGGGGTTCGGGTTGTGCTGTTGAATATAACTGCCAATCACCCCCGCGCCGCAGCCCCCGTc                                                            >  1:849997/1‑250 (MQ=255)                                                                                                                                                                            ttAACGGCGGTGTAAGCGGTGTCAATGCCGTCATGAAAAGGCGGATTGGAAATGATCAGATCGAATTTGCCGTCAATA‑TGGGAAAACACGTCACTGGCAACCACCGTGCCTTGCAACCGATTTTCCTG‑TAAAGTGCGCTTTGCGGAAGCCAACGCCAAGGCGTGAATATCCGTCATGATTAATCGGGTGTTCGGGTTGTGCTGTTGAATATAACTGCCAATCACCCCTGCGCCGCAGCCCACGTCCAacac                                                      <  1:86979/251‑1 (MQ=255)                                                                                                                                                                            ttAACGGCGGTGTAAGCGGTGTCAATGCCGTCATGAAAAGGCGGATTGGAAATGATCAGATCGAATTTGCCGTCAATA‑TGGGAAAACACGTCACTGGCAACCACCGTGCCTTGCAACCGATTTTCCTG‑TAAAGTGCGCTTTGCGGAAGCCAACGCCAAGGCGTGAATATCCGTCATGATTAATCGGGTGTTCGGGTTGTGCTGTTGAATATAACTGCCAATCACCCCTGCGCCGCAGCCCACGTCCAacac                                                      <  1:362688/251‑1 (MQ=255)                                                                                                                                                                                cggcggTGTAAGCGGTGTCAATGCCGTCATGAAAAGGCGGATTGGAAATGATCAGATCGAATTTGCCGTCAATA‑TGGGAAAACACGTCACTGGCAACCACCGTGCCTTGCAACCGATTTTCCTG‑TAAAGTGCGCTTTGCGGAAGCCAACGCCAAGGCGTGAATATCCGTCATGATTAATCGGGTGTTCGGGTTGTGCTGTTGAATATAACTg                                                                                         >  1:323483/1‑212 (MQ=255)                                                                                                                                                                                     gtgtAAGCGGTGTCAATGCCGTCATGAAAAGGCGGATTGGAAATGATCAGATCGAATTTGCCGTCAATA‑TGGGAAAACACGTCACTGGCAACCACCGTGCCTTGCAACCGATTTTCCTG‑TAAAGTgcgc                                                                                                                                                                       >  1:461547/1‑129 (MQ=255)                                                                                                                                                                                     gtgtAAGCGGTGTCAATGCCGTCATGAAAAGGCGGATTGGAAATGATCAGATCGAATTTGCCGTCAATA‑TGGGAAAACACGTCACTGGCAACCACCGTGCCTTGCAACCGATTTTCCTG‑TAAAGTGCGCTTTGCGGAAGCCAACGc                                                                                                                                                      <  1:556513/146‑1 (MQ=255)                                                                                                                                                                                     gtgtAAGCGGTGTCAATGCCGTCATGAAAAGGCGGATTGGAAATGATCAGATCGAATTTGCCGTCAATA‑TGGGAAAACACGTCACTGGCAACCACCGTGCCTTGCAACCGATTTTCCTG‑TAAAGTGCGCTTTGCGGAAGCCAACGCCAAGGCGTGAATATCCGTCATGATTAATCggg                                                                                                                      >  1:379572/1‑178 (MQ=255)                                                                                                                                                                                      tgtAAGCGGTGTCAATGCCGTCATGAAAAGGCGGATTGGAAATGATCAGATCGAATTTGCCGTCAATA‑TGGGAAAACACGTCACTGGCAACCACCGTGCCTTGCAACCGATTTTCCTG‑TAAAGTGCGCTTTGCGGAAGCCAACGCCAAGGCGTGAATATCCGTCATGATTAATCGGGTGTTCGGGTTGTGCTGTTGAATATAACTGCCAATCAcccc                                                                              <  1:440843/217‑4 (MQ=255)                                                                                                                                                                                       gtAAGCGGTGTCAATGCCGTCATGAAAAGGCGGATTGGAAATGATCAGATCGAATTTGCCGTCAATA‑TGGGAAAACACGTCACTGGCAACCACCGTGCCTTGCAACCGATTTTCCTG‑TAAAGTGCGCTTTGCGGAAGCCAACGCCAAGGCGTGAATATCCGTCATGATTAATCGGGTGTTCGGGTTGTGCTGTTGAATATAACTGCCAATCACCCCTGCGCCGCAGCCCACGTCCAacac                                                      >  1:589367/1‑240 (MQ=255)                                                                                                                                                                                              gtgtCAATGCCGTCATGAAAAGGCGGATTGGAAATGATCAGATCGAATTTGCCGTCAATA‑TGGGAAAACACGTCACTGGCAACCACCGTGCCTTGCAACCGATTTTCCTG‑TAAAGTGCGCTTTGCGGAAGCCAACGCCAAGGCGTGAATATCCGTCATGATTAATCGGGTGTTCGGGTTGTGCTGTTGAATATAACTGCCAATCACCCCTGCGCCGCAGCCCACGTCCAACACCTCGCCCGCGATGttttt                                    >  1:274544/1‑251 (MQ=255)                                                                                                                                                                                                gtCAATGCCGTCATGAAAAGGCGGATTGGAAATGATCAGATCGAATTTGCCGTCAATA‑TGGGAAAACACGTCACTGGCAACCACCGTGCCTTGCAACCGATTTTCCTG‑TAAAGTGCGCTTTGCGGAAGCCAACGCCAAGGCGTGAATATCCGTCATg                                                                                                                                <  1:525035/157‑1 (MQ=255)                                                                                                                                                                                                gtCAATGCCGTCATGAAAAGGCGGATTGGAAATGATCAGATCGAATTTGCCGTCAATA‑TGGGAAAACACGTCACTGGCAACCACCGTGCCTTGCAACCGATTTTCCTG‑TAAAGTGCGCTTTGCGGAAGCCAACGCCAAGGCGTGAATATCCGTCATGAtt                                                                                                                             >  1:877830/1‑160 (MQ=255)                                                                                                                                                                                                         gtcatGAAAAGGCGGATTGGAAATGATCAGATCGAATTTGCCGTCAATA‑TGGGAAAACACGTCACTGGCAACCACCGTGCCTTGCAACCGATTTTCCTG‑TAAAGTGCGCTTTGCGGAAGCCAACGCCAAGGCGTGAATATCCGTCATGATTAATCGGGTGTTCGGGTTGTGCTGTTGAATATAACTGCCAATCACCCCTGCGCCGCAGCCCACGTCCAACACCTCGCCCGCGATGTTTTTTAGCGTAGaaa                         >  1:108366/2‑251 (MQ=255)                                                                                                                                                                                                         gtcatGAAAAGGCGGATTGGAAATGATCAGATCGAATTTGCCGTCAATA‑TGGGAAAACACGTCACTGGCAACCACCGTGCCTTGCAACCGATTTTCCTG‑TAAAGTGCGCTTTGCGGAAGCCAACGCCAAGGCGTGAATATCCGTCATGATTAATCGGGTGTTCGGGTTGTGCTGTTGAATATAACTGCCAATCACCCCTGCGCCGCAGCCCACGTCCAACACCTCGCCCGCGATGTTTTTTAGCGTAGaaa                         >  1:534545/2‑251 (MQ=255)                                                                                                                                                                                                           catGAAAAGGCGGATTGGAAATGATCAGATCGAATTTGCCGTCAATA‑TGGGAAAACACGTCACTGGCAACCACCGTGCCTTGCAACCGATTTTCCTG‑TAAAGTGCGCTTTGCGGAAGCCAACGCCAAGGCGTGAATATCCGTCATGATTAATCGGGTGTTCGGGTTGTGCTGTTGAATATAACTGCCAATCACCCCTGCGCCGCAGCCCACGTCCAACACCTCGCCCGCGATGTTTTTTAGCGTAGAAAGc                       <  1:764399/251‑1 (MQ=255)                                                                                                                                                                                                             tGAAAAGGCGGATTGGAAATGATCAGATCGAATTTGCCGTCAATA‑TGGGAAAACACGTCACTGGCAACCACCGTGCCTTGCAACCGATTTTCCTG‑TAAAGTGCGCTTTGCGGAAGCCAACGCCAAGGCGTGAATATCCGTCATGATTAATCGGGTGTTCGGGTTGTGCTGTTGAATATAACTGCCAATCACCCCTgcgc                                                                         <  1:791792/199‑1 (MQ=255)                                                                                                                                                                                                               aaaaggcggATTGGAAATGATCAGATCGAATTTGCCGTCAATA‑TGGGAAAACACGTCACTGGCAACCACCGTGCCTTGCAACCGATTTTCCTG‑TAAAGTGCGCTTTGCGGAAGCCAACGCCAAGGCGTGAATATCCGTCATGATTAATCGGGTGTTCGGGTTGTGCTGTTGAATATAACTGCCAATCACCCCTGCGCCGCAGCCCACGTCCAACACCTCGCCCGCGATGTTTTTTAGCGTAGAAAGCaat                    <  1:278914/246‑1 (MQ=255)                                                                                                                                                                                                                    gcggATTGGAAATGATCAGATCGAATTTGCCGTCAATA‑TGGGAAAACACGTCACTGGCAACCACCGTGCCTTGCAACCGATTTTCCTG‑TAAAGTGCGCTTTGCGGAAGCCAACGCCAAGGCGTGAATATCCGTCATGATTAATCGGGTGTTCGGGTTGTGCTGTTGAATATAACTGCCAATCACCCCTGCGCCGCAGCCCACGTCCAACACCTCGCCCGCGATGTTTTTTAGCGTAGAAAGCAATAAtgc               <  1:142918/250‑1 (MQ=255)                                                                                                                                                                                                                       gattGGAAATGATCAGATCGAATTTGCCGTCAATA‑TGGGAAAACACGTCACTGGCAACCACCGTGCCTTGCAACCGATTTTCCTG‑TAAAGTGCGCTTTGCGGAAGCCAACGCCAAGGCGTGAATATCCGTCATGATTAATCGGGTGTTCGGGTTGTGCTGTTGAatat                                                                                              >  1:283830/3‑168 (MQ=255)                                                                                                                                                                                                                       gattGGAAATGATCAGATCGAATTTGCCGTCAATA‑TGGGAAAACACGTCACTGGCAACCACCGTGCCTTGCAACCGATTTTCCTG‑TAAAGTGCGCTTTGCGGAAGCCAACGCCAAGGCGTGAATATCCGTCATGATTAATCGGGTGTTCGGGTTGTGCTGTTGAATATAACTGCCAATCACCCCTGCGCCGCAGCCCACGTCCAACACCTCGCCCGCGATGTTTTTTAGCGTAGAAAGCAATAAtgccgtg           >  1:93684/3‑251 (MQ=255)                                                                                                                                                                                                                           ggAAATGATCAGATCGAATTTGCCGTCAATA‑TGGGAAAACACGTCACTGGCAACCACCGTGCCTTGCAACCGATTTTCCTG‑TAAAGTGCGCTTTGCGGAAGCCAACGCCAAGGCGTGAATATCCGTCATGATTAATCGGGTGTTCGGGTTGTGCTGTTGAATATAACTGCCAATCACCCCTGCGCCGCAGCCCACGTCCAACACCTCGCCCGCGATGtttt                                     >  1:436831/1‑221 (MQ=255)                                                                                                                                                                                                                            gAAATGATCAGATCGAATTTGCCGTCAATA‑TGGGAAAACACGTCACTGGCAACCACCGTGCCTTGCAACCGATTTTCCTG‑TAAAGTGCGCTTTGCGGAAGCCAACGCCAAGGCGTGAATATCCGTCATGATTAATCGGGTGTTCGGGTTGTGCTGTTGAATATAACTGCCAATCACCCCTGCGCCGCAGCCCACGTCCAACACCTCGCCCGCGATGTTTTTTAGCGTAGAAAGCAATAATGCCGTgccgc       >  1:619016/1‑250 (MQ=255)                                                                                                                                                                                                                                tGATCAGATCGAATTTGCCGTCAATA‑TGGGAAAACACGTCACTGGCAACCACCGTGCCTTGCAACCGATTTTCCTG‑TAAAGTGCGCTTTGCGGAAGCCAACGCCAAGGCGTGAATATCCGTCATGATTAATCGGGTGTTCGGGTTGTGCTGTTGAATATAACTGCCAATCACCCCTGCGCCGCAGCCCACGTCCAACACCTCGCCCGCGATGTTTTTTAGCGTAGAAAGCAATAATGCCGTGCCGCCATcc  <  1:706324/251‑1 (MQ=255)                                                                                                                                                                                                                                tGATCAGATCGAATTTGCCGTCAATA‑TGGGAAAACACGTCACTGGCAACCACCGTGCCTTGCAACCGATTTTCCTG‑TAAAGTGCGCTTTGCGGAAGCCAACGCCAAGGCGTGAATATCCGTCATGATTAATCGGGTGTTCGGGTTGTGCTGTTGAATATAACTGCCAATCACCCCTGCGCCGCAGCCCACGTCCAACACCTCGCCCGCGATGTTTTTTAGCGTAGAAAGCAATAATGCCGTGCCGCCATcc  <  1:893288/251‑1 (MQ=255)                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                   CACTTAACTCATCCGTGCACCGAATAAACCTTAAACTTGTTATTTCTCGCCAGGACGTCATGACTGCCGAAATGTTGATTCAGCCAGTCGGCGTAAGGCAGAAACGCATTTGCCACGATGCGCAGCTCACCGCCCGGTTTCAAATGCCATTTTGCCTGTTTTATCAGTTCGTTAACGGCGGTGTAAGCGGTGTCAATGCCATCATGAAACGGCGGGTTGGAAATGATCAGATCAAATTTGCCGTCAATA‑TGGGAAAACACATCACTGGCAACCACTGTTCCCTGTAGCCGATTTTCCTG‑TAAAGTGCGCTTTGCGGAAGCCAACGCCAAGGCATGAATATCTGTCATGATTAATCGGGTGTTCGGGTTGTGCCGTTGAATATAACTGCCGATCACTCCCGCGCCGCAGCCCACGTCCAACACCCCGCCTGCGATGTTTTTTAGCGTAGAAAGCAATAATGCCGTGCCGCCATCC  >  1/1986299‑1986772

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑