| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | 1 | 1,986,499 | A→G | D271D (GAT→GAC) | unknown ← | 16S rRNA (guanine(1207)‑N(2))‑methyltransferase (EC 2.1.1.172) |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | 1 | 1,986,499 | 0 | A | G | 96.3% | 198.6 / ‑5.3 | 81 | D271D (GAT→GAC) | unknown | 16S rRNA (guanine(1207)‑N(2))‑methyltransferase (EC 2.1.1.172) |
| Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (33/45); minor base C (3/0); total (36/45) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.37e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.09e-01 | |||||||||||
AACTAAAAGGCTTCGCTAAGCCAGCGGAATTTCGTGCACACTTAACTCATCCGTGCACCGAATAAACCTTAAACTTGTTATTTCTCGCCAGGACGTCATGACTGCCGAAATGTTGATTCAGCCAGTCGGCGTAAGGCAGAAACGCATTTGCCACGATGCGCAGCTCACCGCCCGGTTTCAAATGCCATTTTGCCTGTTTTATCAGTTCGTTAACGGCGGTGTAAGCGGTGTCAATGCCATCATGAAACGGCGGGTTGGAAATGATCAGATCAAATTTGCCGTCAATA‑TGGGAAAACACATCACTGGCAACCACTGTTCCCTGTAGCCGATTTTCCTG‑TAAAGTGCGCTTTGCGGAAGCCAACGCCAAGGCATGAATATCTGTCATGATTAATCGGGTGTTCGGGTTGTGCCGTTGAATATAACTGCCGATCACTCCCGCGCCGCAGCCCACGTCCAACACCCCGCCTGCGATGTTTTT > 1/1986261‑1986738 | aactaaAAGGCTTCGCTAAGCCAGCGGAATTTCGTGCACACTTAACTCATCTGCGCACCGAATATACCTTAAACTTGTTATTTCTCGCCAGGACGTCATGACTGCCGAAATGTTGATTCAGCCAGTCGGCGTAAGGCAGAAACGCATTTGCCACGATGCGCAGCTCACCGCCCGGTTTCAAATGCCATTTTGCCTGTTTTATCAGTTCGTTAACGGCGGTGTAAGCGGGGGCAAAGCCGTCATGaaaaagg > 1:796712/1‑247 (MQ=255)aactaaAAGGCTTCGCTAAGCCAGCGGAATTTCGTGCACACTTAACTCATCTGCGCACCGAATATACCTTAAACTTGTTATTTCTCGCCAGGACGTCATGACTGCCGAAATGTTGATTCAGCCAGTCGGCGTAAGGCAGAAACGCATTTGCCACGATGCGCAGCTCACCGCCCGGTTTCAAATGCCATTTTGCCTGTTTTATCAGTTCGTTAACGGCGGTGGTAGCGGTGTCAATGCCGTCATGaaaaagg > 1:310541/1‑247 (MQ=255) ctaaAAGGCTTCGCTAAGCCAGCGGAATTTCGTGCACACTTAACTCATCTGCGCACCGAATATACCTTAAACTTGTTATTTCTCGCCAGGACGTCATGACTGCCGAAATGTTGATTCAGCCAGTCGGCGTAAGGCAGAAACGCATTTGCCACGATGCGCAGCTCACCGCCCGGTTTCAAATGCCATTTTGCCTGTTTTATCAGTTCGTTAACGGCGGTGTAAGCGGTGTCAATGGCGTCATGaaaaggtgg > 1:649459/1‑245 (MQ=255) ctaaAAGGCTTCGCTAAGCCAGCGGAATTTCGTGCACACTTAACTCATCTGCGCACCGAATATACCTTAAACTTGTTATTTCTCGCCAGGACGTCATGACTGCCGAAATGTTGATTCAGCCAGTCGGCGTAAGGCAGAAACGCATTTGCCACGATGCGCAGCTCACCGCCCGGTTTCAAATGCCATTTTGCCTGTTTTATCAGTTCGTTAACGGCGGCGTACGCGGGGTCAATACCGTCCTGaaaaagcgg > 1:315432/1‑245 (MQ=255) cTTCGCTAAGCCAGCGGAATTTCGTGCACACTTAACTCATCTGCGCACCGAATATACCTTAAACTTGTTATTTCTCGCCAGGACGTCATGACTGCCGAAATGTTGATTCAGCCAGTCGGCGTAAGGCAGAAACGCATTTGCCACGATGCGCAGCTCACCGCCCGGTTTCAAATGCCATTTTGCCTGTTTTATCAGTTCGTTAACGGCGGTGTAAGCGGTGTCAATGCCGTCATGAAAAGGCGGATTGGaaa < 1:18630/251‑1 (MQ=255) cGCTAAGCCAGCGGAATTTCGTGCACACTTAACTCATCTGCGCACCGAATATACCTTAAACTTGTTATTTCTCGCCAGGACGTCATGACTGCCGAAATGTTGATTCAGCCAGTCGGCGTAAGGCAGAAACGCATTTGCCACGATGCGCAGCTCACCGCCCGGTTTCAAATGCCATTTTGCCTGTTTTATCAGTTCGTTAACGGCGGTGTAAGCGGGGGCAATGCCGGCATGaaaaagcgggatgggaaaga > 1:731724/1‑234 (MQ=255) cGCTAAGCCAGCGGAATTTCGTGCACACTTAACTCATCTGCGCACCGAATATACCTTAAACTTGTTATTTCTCGCCAGGACGTCATGACTGCCGAAATGTTGATTCAGCCAGTCGGCGTAAGGCAGAAACGCATTTGCCACGATGCGCAGCTCACCGCCCGGTTTCAAATGCCATTTTGCCTGTTTTATCAGTTCGTTAACGGCGGTGTAAGCGGGGGCAAAGCCCGCATGAAAAAGCGGGTTgggaaaga > 1:578588/1‑245 (MQ=255) cTAAGCCAGCGGAATTTCGTGCACACTTAACTCATCTGCGCACCGAATATACCTTAAACTTGTTATTTCTCGCCAGGACGTCATGACTGCCGAAATGTTGATTCAGCCAGTCGGCGTAAGGCAGAAACGCATTTGCCACGATGCGCAGCTCACCGCCCGGTTTCAAATGCCATTTTGCCTGTTTTATCAGTTCGTTAACGGCGGTGTAAGCGGTGTCAATGCCGTCATGAAAAGGCGGATTGGAAATgatc < 1:268844/251‑1 (MQ=255) cTAAGCCAGCGGAATTTCGTGCACACTTAACTCATCTGCGCACCGAATATACCTTAAACTTGTTATTTCTCGCCAGGACGTCATGACTGCCGAAATGTTGATTCAGCCAGTCGGCGTAAGGCAGAAACGCATTTGCCACGATGCGCAGCTCACCGCCCGGTTTCAAATGCCATTTTGCCTGTTTTATCAGTTCGTTAACGGCGGTGTAAGCGGTGTCAATGCCGTCATGAAAAGGCGGATTGGAAATgatc < 1:309317/251‑1 (MQ=255) gCGGAATTTCGTGCACACTTAACTCATCTGCGCACCGAATATACCTTAAACTTGTTATTTCTCGCCAGGACGTCATGACTGCCGAAATGTTGATTCAGCCAGTCGGCGTAAGGCAGAAACGCATTTGCCACGATGCGCAGCTCACCGCCCGGTTTCAAATGCCATTTTGCCTGTTTTATCAGTTCGTTAACGGCGGTGGAAGCGGGGGCCATGCCGTCATGAAAAAGCGGAGTGGGAATgatcagatagaa > 1:597404/1‑247 (MQ=255) tGCACACTTAACTCATCTGCGCACCGAATATACCTTAAACTTGTTATTTCTCGCCAGGACGTCATGACTGCCGAAATGTTGATTCAGCCAGTCGGCGTAAGGCAGAAACGCATTTGCCACGATGCGCAGCTCACCGCCCGGTTTCAAATGCCATTTTGCCTGTTTTATCAGTTCGTTAACGGCGGCGTAAGCGGGGTCCATGCCGTCATGAAAAGGCGGGTTGGGAAAgaacagatc > 1:460007/1‑237 (MQ=255) cacTTAACTCATCTGCGCACCGAATATACCTTAAACTTGTTATTTCTCGCCAGGACGTCATGACTGCCGAAATGTTGATTCAGCCAGTCGGCGTAAGGCAGAAACGCATTTGCCACGATGCGCAGCTCACCGCCCGGTTTCAAATGCCATTTTGCCTGTTTTATCAGTTCGTTAACGGCGGTGTAAGCGGGGTCAATGCCGGCATGAAAAAGCGGGGTGGAAATgatc > 1:719455/1‑228 (MQ=255) cacTTAACTCATCTGCGCACCGAATATACCTTAAACTTGTTATTTCTCGCCAGGACGTCATGACTGCCGAAATGTTGATTCAGCCAGTCGGCGTAAGGCAGAAACGCATTTGCCACGATGCGCAGCTCACCGCCCGGTTTCAAATGCCATTTTGCCTGTTTTATCAGTTCGTTAACGGCGGTGTAAGCGGGGGCAATGCCGGCCTGGAAAGGGGGGTTGGGAATGATTAGATCGAATTTggcggcaaaatg > 1:675427/1‑240 (MQ=255) cacTTAACTCATCTGCGCACCGAATATACCTTAAACTTGTTATTTCTCGCCAGCACGTCATGACTGCCGAAATGTTGATTCAGCCAGTCGGCGTAAGGCAGAAACGCATTTGCCACGATGCGCAGCTCACCGCCCGGTTTCAAATGCCATTTTGCCTGTTTTATCAGTTCGTTAACGGCGGTGTAAGAGGGGGCCATGCCCTCCTGAAAAAGCGGATTTGAAATGATTAGATCGAATTTTCCGTCCATA‑Tg > 1:623715/1‑251 (MQ=255) cTTAACTCATCTGCGCACCGAATATACCTTAAACTTGTTATTTCTCGCCAGGACGTCATGACTGCCGAAATGTTGATTCAGCCAGTCGGCGTAAGGCAGAAACGCATTTGCCACGATGCGCAGCTCACCGCCCGGTTTCAAATGCCATTTTGCCTGTTTTATCAGTTCGTTAACGGCGGTGTAAGCGGTGTCAATGCCGTCATGAAAAGGCGGATTGGAAATGATCAGATCGAATTTGCCGTCAATA‑Tggg < 1:66558/251‑1 (MQ=255) cTTAACTCATCTGCGCACCGAATATACCTTAAACTTGTTATTTCTCGCCAGGACGTCATGACTGCCGAAATGTTGATTCAGCCAGTCGGCGTAAGGCAGAAACGCATTTGCCACGATGCGCAGCTCACCGCCCGGTTTCAAATGCCATTTTGCCTGTTTTATCAGTTCGTTAACGGCGGTGTAAGCGGTGTCAATGCCGTCATGAAAAGGCGGATTGGAAATGATCAGATCGAATTTGCCGTCAATA‑Tggg < 1:829198/251‑1 (MQ=255) cTTAACTCATCTGCGCACCGAATATACCTTAAACTTGTTATTTCTCGCCAGGACGTCATGACTGCCGAAATGTTGATTCAGCCAGTCGGCGTAAGGCAGAAACGCATTTGCCACGATGCGCAGCTCACCGCCCGGTTTCAAATGCCATTTTGCCTGTTTTATCAGTTCGTTAACGGCGGTGTAAGCGGGGTCAATGCCCTCACTAAAAGGCGGATTGGAAATGATCAGATCGAATTTTCCGTCAATA‑Tggg > 1:702141/1‑251 (MQ=255) tAACTCATCTGCGCACCGAATATACCTTAAACTTGTTATTTCTCGCCAGGACGTCATGACTGCCGAAATGTTGATTCAGCCAGTCGGCGTAAGGCAGAAACGCATTTGCCACGATGCGCAGCTCACCGCCCGGTTTCAAATGCCATTTTGCCTGTTTTATCAGTTCGTTAACGGCGGTGTAAGCGGTGTCAATGCCGTCATGAAAAGGCGGATTGGAAATgatc < 1:31835/224‑1 (MQ=255) tAACTCATCTGCGCACCGAATATACCTTAAACTTGTTATTTCTCGCCAGGACGTCATGACTGCCGAAATGTTGATTCAGCCAGTCGGCGTAAGGCAGAAACGCATTTGCCACGATGCGCAGCTCACCGCCCGGTTTCAAATGCCATTTTGCCTGTTTTATCAGTTCGTTAACGGCGGTGTAAGCGGTGTCAATGCCGTCATGAAAAGGCGGATTGGAAATGATCAGATCGAATTTGCCGTCAATA‑TGGGaa < 1:729665/251‑1 (MQ=255) tAACTCATCTGCGCACCGAATATACCTTAAACTTGTTATTTCTCGCCAGGACGTCATGACTGCCGAAATGTTGATTCAGCCAGTCGGCGTAAGGCAGAAACGCATTTGCCACGATGCGCAGCTCACCGCCCGGTTTCAAATGCCATTTTGCCTGTTTTATCAGTTCGTTAACGGCGGTGTAAGCGGTGTCAATGCCGTCATGAAAAGGCGGATTGGAAATGATCAGATCGAATTTGCCGTCAATA‑TGGGaa < 1:800969/251‑1 (MQ=255) cTCATCTGCGCACCGAATATACCTTAAACTTGTTATTTCTCGCCAGGACGTCATGACTGCCGAAATGTTGATTCAGCCAGTCGGCGTAAGGCAGAAACGCATTTGCCACGATGCGCAGCTCACCGCCCGGTTTCAAATGCCATTTTGCCTGTTTTATCAGTTCGTTAACGGCGGTGTAAGCGGTGTCAATGCCGTCATGAAAAGGCGGATTGGAAATGATCAGATCGAATTTGCCGTCAATA‑TGGGAAAac < 1:739006/251‑1 (MQ=255) tctgcgCACCGAATATACCTTAAACTTGTTATTTCTCGCCAGGACGTCATGACTGCCGAAATGTTGATTCAGCCAGTCGGCGTAAGGCAGAAACGCATTTGCCACGATGCGCAGCTCACCGCCCGGTTTCAAATGCCATTTTGCCTGTTTTATCAGTTCGTTAACGGCGGTGGAAGCGGGGTCAATGGCGTCATGAAAAAGCGGGTTGGGAATGATCAGATCGAATTTGGCGGCAATA‑TGGGaaaaaacgg > 1:318/6‑246 (MQ=255) gCACCGAATATACCTTAAACTTGTTATTTCTCGCCAGGACGTCATGACTGCCGAAATGTTGATTCAGCCAGTCGGCGTAAGGCAGAAACGCATTTGCCACGATGCGCAGCTCACCGCCCGGTTTCAAATGCCATTTTGCCTGTTTTATCAGTTCGTTAACGGCGGTGGAAGGGGTGGCAATGCCGTCATggaaaagcgg > 1:352990/1‑190 (MQ=255) cACCGAATATACCTTAAACTTGTTATTTCTCGCCAGGACGTCATGACTGCCGAAATGTTGATTCAGCCAGTCGGCGTAAGGCAGAAACGCATTTGCCACGATGCGCAGCTCACCGCCCGGTTTCAAATGCCATTTTGCCTGTTTTATCAGTTCGTTAACGGCGGTGTAAGCGGTGTCAATGCCGTCATGAAAAGGCGGATTGGAAATGATCAGATCGAATTTGCCGTCAATA‑TGGGAAAACACGTCACTgg < 1:740646/251‑1 (MQ=255) cACCGAATATACCTTAAACTTGTTATTTCTCGCCAGGACGTCATGACTGCCGAAATGTTGATTCAGCCAGTCGGCGTAAGGCAGAAACGCATTTGCCACGATGCGCAGCTCACCGCCCGGTTTCAAATGCCATTTTGCCTGTTTTATCAGTTCGTTAACGGCGGTGTAAGCGGTGTCAATGCCGTCATGAAAAGGCGGATTGGAAATGATCAGATCGAATTTGCCGTCAATA‑TGGGAAAACACGTCACTgg < 1:475818/251‑1 (MQ=255) ccGAATATACCTTAAACTTGTTATTTCTCGCCAGGACGTCATGACTGCCGAAATGTTGATTCAGCCAGTCGGCGTAAGGCAGAAACGCATTTGCCACGATGCGCAGCTCACCGCCCGGTTTCAAATGCCATTTTGCCTGTTTTATCAGTTCGTTAACGGCGGTGTAAGCGGTGTCAATGCCGTCATGAAAAGGCGGATTGGAAATGATCAGATCGAATTTGCCGTCAATA‑Tggg < 1:782848/234‑1 (MQ=255) ccGAATATACCTTAAACTTGTTATTTCTCGCCAGGACGTCATGACTGCCGAAATGTTGATTCAGCCAGTCGGCGTAAGGCAGAAACGCATTTGCCACGATGCGCAGCTCACCGCCCGGTTTCAAATGCCATTTTGCCTGTTTTATCAGTTCGTTAACGGCGGTGTAAGCGGGGTCAATGCCGTCATGAAAAGGCGGGTTGGGAATGATCAGATCGAAATTGCCGTCAATA‑TGGGGAAAAACGTCACTGGCa > 1:327429/1‑251 (MQ=255) ccGAATATACCTTAAACTTGTTATTTCTCGCCAGGACGTCATGACTGCCGAAATGTTGATTCAGCCAGTCGGCGTAAGGCAGAAACGCATTTGCCACGATGCGCAGCTCACCGCCCGGTTTCAAATGCCATTTTGCCTGTTTTATCAGTTCGTTAACGGCGGTGGAAGCGGTGTCCATGCCGTCCTTAAAAAGCGGGTTGGAAATTATCAGATCGAATTTTCCGTCCATA‑TTGGAAAACACACCACTGGCa > 1:887063/1‑251 (MQ=255) tataCCTTAAACTTGTTATTTCTCGCCAGGACGTCATGACTGCCGAAATGTTGATTCAGCCAGTCGGCGTAAGGCAGAAACGCATTTGCCACGATGCGCAGCTCACCGCCCGGTTTCAAATGCCATTTTGCCTGTTTTATCAGTTCGTTAACGGCGGTGTAAGCGGTGTCAATGCCGTCATGAAAAGGCGGATTGGAAATGATCAGATCGAATTTGCCGTCAATA‑TGGGAAAACACGTCACTGGCAACCAc < 1:235068/248‑1 (MQ=255) aCCTTAAACTTGTTATTTCTCGCCAGGACGTCATGACTGCCGAAATGTTGATTCAGCCAGTCGGCGTAAGGCAGAAACGCATTTGCCACGATGCGCAGCTCACCGCCCGGTTTCAAATGCCATTTTGCCTGTTTTATCAGTTCGTTAACGGCGGTGTAAGCGGGGTCAATGCCGTCCTGGAAAGGGGGGTTGGGAATGATCAGATCGAATTTGCCGGCAATATTGGGAAAA‑AAGTCACTGGGAACCAccgg > 1:224597/1‑248 (MQ=255) cTTAAACTTGTTATTTCTCGCCAGGACGTCATGACTGCCGAAATGTTGATTCAGCCAGTCGGCGTAAGGCAGAAACGCATTTGCCACGATGCGCAGCTCACCGCCCGGTTTCAAATGCCATTTTGCCTGTTTTATCAGTTCGTTAACGGCGGTGTAAGCGGTGTCAATGCCGTCATGAAAAGGCGGATTGGAAATGATCAGATCGAATTTGCCGTCAATA‑TGGGAAAACACGTCACTGGCAACCAccgtgc < 1:836938/251‑6 (MQ=255) aTTTCTCGCCAGGACGTCATGACTGCCGAAATGTTGATTCAGCCAGTCGGCGTAAGGCAGAAACGCATTTGCCACGATGCGTAGCTCACCGCCCGGTTTCAAATGCCATTTTGCCTGTTTTATCAGTTCGTTAACGGCGGTGTAAGCGGTGTCAATGCCGTCATGAAAAGGCGGATTGGAAATGATCAGATCGAATTTGCCGTCAATA‑TGGGAAAACACGTCACTGGCAACCAccgtgccttgcaaccgat < 1:448429/251‑18 (MQ=255) cGCCAGGACGTCATGACTGCCGAAATGTTGATTCAGCCAGTCGGCGTAAGGCAGAAACGCATTTGCCACGATGCGCAGCTCACCGCCCGGTTTCAAATGCCATTTTGCCTGTTTTATCAGTTCGTTAACGGCGGTGTAAGCGGTGTCAATGCCGTCATGAAAAGGCGGATTGGAAATgatcagat < 1:608175/185‑1 (MQ=255) cGCCAGGACGTCATGACTGCCGAAATGTTGATTCAGCCAGTCGGCGTAAGGCAGAAACGCATTTGCCACGATGCGCAGCTCACCGCCCGGTTTCAAATGCCATTTTGCCTGTTTTATCAGTTCGTTAACGGCGGTGTAAGCGGTGTCAATGCCGTCATGAAAAGGCGGATTGGAAATGATCAGATCGAATTTGCCGTCAATA‑TGGGAAAACACGTCACTGGCAACCAc < 1:630124/228‑1 (MQ=255) aCGTCATGACTGCCGAAATGTTGATTCAGCCAGTCGGCGTAAGGCAGAAACGCATTTGCCACGATGCGCAGCTCACCGCCCGGTTTCAAATGCCATTTTGCCTGTTTTATCAGTTCGTTAACGGCGGTGTAAGCGGTGTCAATGCCGTCATGAAAAGGCGGATTGGAAATGATCAGATCGAATTTGCCGTCAATA‑TGGGAAAACACGTCACTGGCAACCACCGTGCCTTGCAACCGATTTTCCTG‑TAAAg < 1:386539/250‑1 (MQ=255) aCGTCATGACTGCCGAAATGTTGATTCAGCCAGTCGGCGTAAGGCAGAAACGCATTTGCCACGATGCGCAGCTCACCGCCCGGTTTCAAATGCCATTTTGCCTGTTTTATCAGTTCGTTAACGGCGGTGTAAGCGGTGTCAATGCCGTCATGAAAAGGCGGATTGGAAATGATCAGATCGAATTTGCCGTCAATA‑TGGGAAAACACGTCACTGGCAACCACCGTGCCTTGCAACCGATTTTCCTG‑TAAAg < 1:506731/250‑1 (MQ=255) cGTCATGACTGCCGAAATGTTGATTCAGCCAGTCGGCGTAAGGCAGAAACGCATTTGCCACGATGCGCAGCTCACCGCCCGGTTTCAAATGCCATTTTGCCTGTTTTATCAGTTCGTTAACGGCGGTGTAAGCGGTGTCAATGCCGTCATGAAAAGGCGGATTGGAAATGATCAGATCGAATTTGCCGTCAATA‑Tggg < 1:121830/198‑1 (MQ=255) cTGCCGAAATGTTGATTCAGCCAGTCGGCGTAAGGCAGAAACGCATTTGCCACGATGCGCAGCTCACCGCCCGGTTTCAAATGCCATTTTGCCTGTTTTATCAGTTCGTTAACGGCGGTGTAAGCGGTGTCAATGCCGTCATGAAAAGGCGGATTGGAAATGATCAGATCGAATTTGCCGTCAATA‑TGGGaa < 1:621521/192‑1 (MQ=255) tGTTGATTCAGCCAGTCGGCGTAAGGCAGAAACGCATTTGCCACGATGCGCAGCTCACCGCCCGGTTTCAAATGCCATTTTGCCTGTTTTATCAGTTCGTTAACGGCGGTGTAAGCGGTGTCAATGCCGTCATGAAAAGGCGGATTGGAAATGATCAGATCGAATTTGCCGTCAATA‑Tggg < 1:334201/181‑1 (MQ=255) gATTCAGCCAGTCGGCGTAAGGCAGAAACGCATTTGCCACGATGCGCAGCTCACCGCCCGGTTTCAAATGCCATTTTGCCTGTTTTATCAGTTCGTTAACGGCGGTGTAAGCGGTGTCAATGCCGTCATGAAAAGGCGGATTgg < 1:314511/144‑1 (MQ=255) aaGGCAGAAACGCATTTGCCACGATGCGCAGCTCACCGCCCGGTTTCAAATGCCATTTTGCCTGTTTTATCAGTTCGTTAACGGCGGTGTAAGCGGTGTCAATGCCGTCATGAAAAGGCGGATTGGAAATGATCAGATCGAATTTGCCGTCAATA‑TGGGAAAACACGTCACTGGCAACCACCGTGCCTTGCAACCGATTTTCCTG‑TAAAGTGCGCTTTGCGGAAGCCAACGCCAAGGCGTGAATATCCGTc < 1:626801/251‑1 (MQ=255) gAAACGCATTTGCCACGATGCGCAGCTCACCGCCCGGTTTCAAATGCCATTTTGCCTGTTTTATCAGTTCGTTAACGGCGGTGTAAGCGGTGTCAATGCCGTCATGAAAAGGCGGATTGGAAATGATCAGATCGAATTTGCCGTCAATA‑TGGGAAAACACGTCACTGGCAACCACCGTGCCTTGCAACCGATTTTCCTG‑TAAAGTGCGCTTTGCGGAAGCCAACGCCAAGGCGTGAat < 1:601032/238‑1 (MQ=255) gAAACGCATTTGCCACGATGCGCAGCTCACCGCCCGGTTTCAAATGCCATTTTGCCTGTTTTATCAGTTCGTTAACGGCGGTGTAAGCGGGGTCAATGCCGTCATGAAAAAGCGGATTGGGAATGATCAGATCGAATTTTCCGTCAATA‑TGGGAAAAAACGTCACTGGCAACCACCGGGCCTTGCAACCGATTTTTCTG‑TAAAg > 1:77823/1‑204 (MQ=255) aaaCGCATTTGCCACGATGCGCAGCTCACCGCCCGGTTTCAAATGCCATTTTGCCTGTTTTATCAGTTCGTTAACGGCGGTGTAAGCGGTGTCAATGCCGTCATGAAAAGGCGGATTGGAAATGATCAGATCGAATTTGCCGTCAATA‑TGGGAAAACACGTCACTGGCAACCACCGTGCCTTGCAACCGATTTTCCTG‑TAAAGTGCGCTTTGCGGAAGCCAACGCCAAGGCGTGAATATCCGTCATGa < 1:806713/248‑1 (MQ=255) aaaCGCATTTGCCACGATGCGCAGCTCACCGCCCGGTTTCAAATGCCATTTTGCCTGTTTTATCAGTTCGTTAACGGCGGTGTAAGCGGTGTCAATGCCGTCATGAAAAGGCGGATTGGAAATGATCAGATCGAATTTGCCGTCAATA‑TGGGAAAACACGTCACTGGCAACCACCGTGCCTTGCAACCGATTTTCCTG‑TAAAGTGCGCTTTGCGGAAGCCAACGCCAAGGCGTGAATATCCGTCATGAtt < 1:762075/250‑1 (MQ=255) cATTTGCCACGATGCGCAGCTCACCGCCCGGTTTCAAATGCCATTTTGCCTGTTTTATCAGTTCGTTAACGGCGGTGTAAGCGGTGTCAATGCCGTCATGAAAAGGCGGATTGGAAATGATCAGATCGAATTTGCCGTCAATA‑Tggg < 1:803955/147‑1 (MQ=255) gCCACGATGCGCAGCTCACCGCCCGGTTTCAAATGCCATTTTGCCTGTTTTATCAGTTCGTTAACGGCGGTGTAAGCGGGGTCAATGCCGTCATGAAAAGGCGGGTTGGGAATGATCAGATCGAATTTGCCGTCAATA‑TGGGGAAACAAGTCACTGGCAACCACCCTGCCTTGCAACCGATTTTTCTG‑TAAAATGCGCTTTGCGGAAGCCAACGCCAAGGCGGGAATATCCGTCATGATTAATCGGGGGtt > 1:597229/1‑251 (MQ=255) ccACGATGCGCAGCTCACCGCCCGGTTTCAAATGCCATTTTGCCTGTTTTATCAGTTCGTTAACGGCGGTGTAAGCGGTGTCAATGCCGTCATGAAAAGGCGGATTGGAAATGATCAGATCGAATTTGCCGTCAATA‑TGGGAAAACACGTCACTGGCAACCAc < 1:654530/163‑1 (MQ=255) cGATGCGCAGCTCACCGCCCGGTTTCAAATGCCATTTTGCCTGTTTTATCAGTTCGTTAACGGCGGTGTAAGCGGTGTCAATGCCGTCATGAAAAGGCGGATTGGAAATGATCAGATCGAATTTGCCGTCAATA‑TGGGAAAACACGTCACTGGCAACCACCGTGCCTTGCAACCGATTTTCCTG‑TAAAGTGCGCTTTGCGGAAGCCAACGCCAAGGCGTGAATATCCGTCATGATTAATCGGGTGTTCggg < 1:25843/251‑1 (MQ=255) aTGCGCAGCTCACCGCCCGGTTTCAAATGCCATTTTGCCTGTTTTATCAGTTCGTTAACGGCGGTGTAAGCGGTGTCAATGCCGTCATGAAAAGGCGGATTGGAAATGATCAGATCGAATTTGCCGTCAATA‑TGGGAAAACACGTCACTGGCAACCACCGTGCCTTGCAACCGATTTTCCTG‑TAAAGTGCGCTTTGCGGAAGCCAACGCCAAGGCGTGAATATCCGTCATGATTAATCGGGTGTTCGGGt < 1:2235/250‑1 (MQ=255) aTGCGCAGCTCACCGCCCGGTTTCAAATGCCATTTTGCCTGTTTTATCAGTTCGTTAACGGCGGTGGAAGCGGGGTCAAAGGCGTCATGAAAAGGGGGGATGGGAAAGATTAAAACGAATTTGGCGTTAATA‑TGGGGAAACACGGTACCGGGAACCACCCGGCCCTGCAGCCGATTTTTCTGTTAAAG‑GCGCCTTGCGGGAGCCAACGCCAAGGCGGGGAAATTCGTCATGATTAATCggggggttggggt > 1:456001/1‑241 (MQ=255) gcgcAGCTCACCGCCCGGTTTCAAATGCCATTTTGCCTGTTTTATCAGTTCGTTAACGGCGGTGTAAGCGGTGTCAATGCCGTCATGAAAAAGCGGGTTGGAAATGATCAGATCGAATTTGCCGTCAATA‑TGGGAAAAAACGTCACTGGGAACCACCGTGCCTTGCAACCGCTTTTCCTG‑TAAAGTGCGCTTTGCGGGAGCCAACGCCAAGGCGTGGATATCCGTCCTGATTAATctgggggtcgggg > 1:652145/1‑236 (MQ=255) gcgcAGCTCACCGCCCGGTTTCAAATGCCATTTTGCCTGTTTTATCAGTTCGTTAACGGCGGTGTAAGCGGGGTCAATGCCGTCATGAAAAGGCGGATTGGGAATGATCAGATTGAATTTTCCGGCAATA‑TGGGGAAAAACGGCAATGGCAACCACCCTGCCTTGCAACCGATTTTTCTG‑TAAAGTGCGCTTTGCGGGAGCCAACGCCCAGGGGGGAATATTCCTCATGATTAATCGGGGGTTCggggtgg > 1:56099/1‑247 (MQ=255) cAGCTCACCGCCCGGTTTCAAATGCCATTTTGCCTGTTTTATCAGTTCGTTAACGGCGGTGTAAGCGGTGTCAATGCCGTCATGAAAAGGCGGATTGGAAATGATCAGATCGAATTTGCCGTCAATA‑TGGGAAAACACGTCACTGGCAACCACCGTGCCTTGCAACCGATTTTCCTG‑TAAAGTGCGCTTTGCGGAAGCCAACGCCAAGGCGTGAATATCCGTCATGATTAATCGGGTGTTCGGGTTGTGc < 1:116878/250‑1 (MQ=255) cAGCTCACCGCCCGGTTTCAAATGCCATTTTGCCTGTTTTATCAGTTCGTTAACGGCGGTGTAAGCGGTGTCAATGCCGTCATGAAAAGGCGGATTGGAAATGATCAGATCGAATTTGCCGTCAATA‑TGGGAAAACACGTCACTGGCAACCACCGTGCCTTGCAACCGATTTTCCTG‑TAAAGTGCGCTTTGCGGAAGCCAACGCCAAGGCGTGAATATCCGTCATGATTAATCGGGTGTTCGGGTTGTGc < 1:725967/250‑1 (MQ=255) gCTCACCGCCCGGTTTCAAATGCCATTTTGCCTGTTTTATCAGTTCGTTAACGGCGGTGTAAACGGGGGCAATGCCGTCCTTAAAAAGCGGGTTGGGAAAGATCAGATCGAATTTGCCGGCCATA‑TGGGGAAAAACGTCACTGGCCACCCCCGGGCCTTTCAACCGATTTTTCTG‑TAAAGAGCGCTTTGCGGGAGCCCACGCCAAGGCGTGAATATCCGTCCTGATTAATCGGGGGTTCGGGGTGTGctgg > 1:889829/1‑248 (MQ=255) cccGGTTTCAAATGCCATTTTGCCTGTTTTATCAGTTCGTTAACGGCGGTGTAAGCGGTGTCAATGCCGTCATGAAAAGGCGGATTGGAAATGATCAGATCGAATTTGCCGTCAATA‑TGGGAAAACACGTCACTGGCAACCACCGTGCCTTGCAACCGATTTTCCTG‑TAAAGTGCGCTTTGCGGAAGCCAACGCCAAGGCGTGAATATCCGTCATGATTAATCGGGTGTTCGGGTTGTGCTGTTGAatat < 1:294368/250‑1 (MQ=255) cGGTTTCAAATGCCATTTTGCCTGTTTTATCAGTTCGTTAACGGCGGTGTAAGCGGTGTCAATGCCGTCATGAAAAGGCGGATTGGAAATGATCAGATCGAATTTGCCGTCAATA‑TGGGAAAACACGTCACTGGCAACCACCGTGCCTTGCAACCGATTTTCCTG‑TAAAGTGCGCTTTGCGGAAGCCAACGCCAAGGCGTGAATATCCGTCATGATTAATCGGGTGTTCGGGTTGTGCTGTTGAATATAAc < 1:763838/251‑1 (MQ=255) ttCAAATGCCATTTTGCCTGTTTTATCAGTTCGTTAACGGCGGTGTAAGCGGGGTCAATGCCGTCCTGAAAAGGCGGGTTGGGAATGATCAGATCGAATTTTCCGTCAATA‑TGGGGAAAAACGTCACTGGCAACCACCCTGCCTTGCAAACGATTTTCCTG‑TAAAATGCGCTTTTCGGAAGACAACGCCAAGGCGGGAATATCCGTCATGATTAAACGGGGGTTCGGGGTGTGCTGTTGAATATAAATGc > 1:830181/1‑250 (MQ=255) cAAATGCCATTTTGCCTGTTTTATCAGTTCGTTAACGGCGGTGTAAGCGGGGTCAAAGCCGTCATGAAAAGGGGGATTGGGAATGAAAAGATCGAATTTGCCGTCAATA‑TGGGAAAACAAGACACTGGCAACCACCGTGCCCTGCAAACGATTTTCCTG‑TAAAGTGCGCTTTTTGGAAGCCAACGCCAAGGCGGGGATATCCTTCATGGTTAAATGGGTGGTCGGGTTGTGCTGTTGTATATAAATGccaa > 1:220091/1‑249 (MQ=255) aTGCCATTTTGCCTGTTTTATCAGTTCGTTAACGGCGGTGTAAGCGGTGTCAATGCCGTCATGAAAAGGCGGATTGGAAATGATCAGATCGAATTTGCCGTCAATA‑TGGGAAAACACGTCACTGGCAACCACCGTGCCTTGCAACCGATTTTCCTG‑TAAAGTGCGCTTTGCGGAAGCCAACGCCAAGGCGTGAATATCCGTCATGATTAATCggg < 1:673398/215‑1 (MQ=255) ccATTTTGCCTGTTTTATCAGTTCGTTAACGGCGGTGTAAGCGGTGTCAATGCCGTCATGAAAAGGCGGATTGGAAATGATCAGATCGAATTTGCCGTCAATA‑TGGGAAAACACGTCACTGGCAACCAc < 1:631068/129‑1 (MQ=255) cATTTTGCCTGTTTTATCAGTTCGTTAACGGCGGTGTAAGCGGTGTCAATGCCGTCATGAAAAGGCGGATTGGAAATGATCAGATCGAATTTGCCGTCAATA‑TGGGAAAACACGTCACTGGCAACCACCGTGCCTTGCAACCGATTTTCCTG‑TAAAGTGCGCTTTGCGGAAGCCAACGCCAAGGCGTGAATATCCGTCATGATTAATCGGGTGTTCGGGTTGTGCTGTTGAATATAACTGCCAATCAcccc < 1:549221/251‑4 (MQ=255) cATTTTGCCTGTTTTATCAGTTCGTTAACGGCGGTGTAAGCGGTGTCAATGCCGTCATGAAAAGGCGGATTGGAAATGATCAGATCGAATTTGCCGTCAATA‑TGGGAAAACACGTCACTGGCAACCACCGTGCCTTGCAACCGATTTTCCTG‑TAAAGTGCGCTTTGCGGAAGCCAACGCCAAGGCGTGAATATCCGTCATGATTAATCGGGTGTTCGGGTTGTGCTGTTGAATATAACTGCCAATCAcccc < 1:850484/251‑4 (MQ=255) cATTTTGCCTGTTTTATCAGTTCGTTAACGGCGGTGTAAGCGGGGTCAATGTCGTCCTGAAAAGGCGGGTTGGGAAAGATCAGATCGAATTTTTCGTCAATA‑TGGGAAAAAACGTCACTGGCAACCACCGCGCCTTGCAAACGATTTTTCTG‑TTAAGTGCGCTTTTCGGGAGCCAAAGCCAAGGCGGGAATATCCGTCATGATTAATCGGGGGTTCGGGGTGTGCTGTTGAATATAAATGCCAATCAccc > 1:479260/1‑248 (MQ=255) gTTTTATCAGTTCGTTAACGGCGGTGTAAGCGGGGTCAATGCCGTCATGAAAAGGCGGGTTGGGAAAGATCAGATCGAATTTGCCGTCAATA‑TGGGGAAACACGTCCCTGGCAAACACCCTGCCTTGCAACCGATTTTTCTG‑TAAAGGGCGCTTTGCGGAAGCCAACGCCAAGGCGGGAATATCCGTCCTGATTAATCGGGTGTTCGGGGTGTGCTGTTGGatat > 1:316686/1‑225 (MQ=255) gTTTTATCAGTTCGTTAACGGCGGTGTAAGCGGGGTCAAAGCCGTCATGAAAAAGCGGGTTGGAAAAGAACAAATCGAATTTGCCGTCAAAA‑AGGGGAAAAACGTCACTGGCAAACACCGTGCCCTGCAACCGATTTTTCTG‑TAAAGAGCGCTTTGCGGAAGCCAACGCCCAGGCGTGAATATCCGGCATGATTAATCGGGGGTTCGGGGTGGGCTGTTGAATATAAATGCCAATCAACCCTGCGCCGCAg > 1:482006/1‑251 (MQ=255) ttATCAGTTCGTTAACGGCGGTGTAAGCGGTGTCAATGCCGTCATGAAAAGGCGGATTGGAAATGATCAGATCGAATTTGCCGTCAATA‑TGGGAAAACACGTCACTGGCAACCACCGTGCCTTGCAACCGATTTTCCTG‑TAAAGTGCGCTTTGCGGAAGCCAACGCCAAGGCGTGAATATCCGTCATGATTAATCGGGTGTTCGGGTTGTGCTGTTGAATATAACTGCCAATCACCCCTGCGCCGCAGccc < 1:358242/251‑1 (MQ=255) tATCAGTTCGTTAACGGCGGTGTAAGCGGGGTCAATGCCGTCCTGAAAAAGCGGGTTGGGAATGATCAGATCGAATTTTCCGTCAATA‑TTGGGAAACACGTCACTGGCAACCACCCTGCCCTGCAACCGATTTTTCTT‑TAAAATGCGCTTTTCGGGAGCCAACGCCCAGGCGGGGATATCCCTCCTGATTAATCTGGGGTTCGGGGTTTGCTGTTGAATATAAATGCCCATCACCCCCGcgcccccgccc > 1:140971/1‑243 (MQ=255) gTTCGTTAACGGCGGTGTAAGCGGGGTCAATGCCGTCATGAAAAAGCGGATTGGGAATGATCAGATCGAATTTGCCGTCCATA‑TGGGGAAACACGTCACTGGCAACCACCCTGCCTTGCAACCGATTTTCCTG‑TAAAGTGCGCTTTGCGGGAGCCAACGCCCAGGCGTGAATATCCGTCATGATTAATCGGGGGTTCGGGTTGTGCTGTTGAATATAACTGCCAATCACCCCCGCGCCGCAGCCCCCGTc > 1:849997/1‑250 (MQ=255) ttAACGGCGGTGTAAGCGGTGTCAATGCCGTCATGAAAAGGCGGATTGGAAATGATCAGATCGAATTTGCCGTCAATA‑TGGGAAAACACGTCACTGGCAACCACCGTGCCTTGCAACCGATTTTCCTG‑TAAAGTGCGCTTTGCGGAAGCCAACGCCAAGGCGTGAATATCCGTCATGATTAATCGGGTGTTCGGGTTGTGCTGTTGAATATAACTGCCAATCACCCCTGCGCCGCAGCCCACGTCCAacac < 1:362688/251‑1 (MQ=255) ttAACGGCGGTGTAAGCGGTGTCAATGCCGTCATGAAAAGGCGGATTGGAAATGATCAGATCGAATTTGCCGTCAATA‑TGGGAAAACACGTCACTGGCAACCACCGTGCCTTGCAACCGATTTTCCTG‑TAAAGTGCGCTTTGCGGAAGCCAACGCCAAGGCGTGAATATCCGTCATGATTAATCGGGTGTTCGGGTTGTGCTGTTGAATATAACTGCCAATCACCCCTGCGCCGCAGCCCACGTCCAacac < 1:86979/251‑1 (MQ=255) cggcggTGTAAGCGGTGTCAATGCCGTCATGAAAAGGCGGATTGGAAATGATCAGATCGAATTTGCCGTCAATA‑TGGGAAAACACGTCACTGGCAACCACCGTGCCTTGCAACCGATTTTCCTG‑TAAAGTGCGCTTTGCGGAAGCCAACGCCAAGGCGTGAATATCCGTCATGATTAATCGGGTGTTCGGGTTGTGCTGTTGAATATAACTg > 1:323483/1‑212 (MQ=255) gtgtAAGCGGTGTCAATGCCGTCATGAAAAGGCGGATTGGAAATGATCAGATCGAATTTGCCGTCAATA‑TGGGAAAACACGTCACTGGCAACCACCGTGCCTTGCAACCGATTTTCCTG‑TAAAGTgcgc > 1:461547/1‑129 (MQ=255) gtgtAAGCGGTGTCAATGCCGTCATGAAAAGGCGGATTGGAAATGATCAGATCGAATTTGCCGTCAATA‑TGGGAAAACACGTCACTGGCAACCACCGTGCCTTGCAACCGATTTTCCTG‑TAAAGTGCGCTTTGCGGAAGCCAACGc < 1:556513/146‑1 (MQ=255) gtgtAAGCGGTGTCAATGCCGTCATGAAAAGGCGGATTGGAAATGATCAGATCGAATTTGCCGTCAATA‑TGGGAAAACACGTCACTGGCAACCACCGTGCCTTGCAACCGATTTTCCTG‑TAAAGTGCGCTTTGCGGAAGCCAACGCCAAGGCGTGAATATCCGTCATGATTAATCggg > 1:379572/1‑178 (MQ=255) tgtAAGCGGTGTCAATGCCGTCATGAAAAGGCGGATTGGAAATGATCAGATCGAATTTGCCGTCAATA‑TGGGAAAACACGTCACTGGCAACCACCGTGCCTTGCAACCGATTTTCCTG‑TAAAGTGCGCTTTGCGGAAGCCAACGCCAAGGCGTGAATATCCGTCATGATTAATCGGGTGTTCGGGTTGTGCTGTTGAATATAACTGCCAATCAcccc < 1:440843/217‑4 (MQ=255) gtAAGCGGTGTCAATGCCGTCATGAAAAGGCGGATTGGAAATGATCAGATCGAATTTGCCGTCAATA‑TGGGAAAACACGTCACTGGCAACCACCGTGCCTTGCAACCGATTTTCCTG‑TAAAGTGCGCTTTGCGGAAGCCAACGCCAAGGCGTGAATATCCGTCATGATTAATCGGGTGTTCGGGTTGTGCTGTTGAATATAACTGCCAATCACCCCTGCGCCGCAGCCCACGTCCAacac > 1:589367/1‑240 (MQ=255) gtgtCAATGCCGTCATGAAAAGGCGGATTGGAAATGATCAGATCGAATTTGCCGTCAATA‑TGGGAAAACACGTCACTGGCAACCACCGTGCCTTGCAACCGATTTTCCTG‑TAAAGTGCGCTTTGCGGAAGCCAACGCCAAGGCGTGAATATCCGTCATGATTAATCGGGTGTTCGGGTTGTGCTGTTGAATATAACTGCCAATCACCCCTGCGCCGCAGCCCACGTCCAACACCTCGCCCGCGATGttttt > 1:274544/1‑251 (MQ=255) gtCAATGCCGTCATGAAAAGGCGGATTGGAAATGATCAGATCGAATTTGCCGTCAATA‑TGGGAAAACACGTCACTGGCAACCACCGTGCCTTGCAACCGATTTTCCTG‑TAAAGTGCGCTTTGCGGAAGCCAACGCCAAGGCGTGAATATCCGTCATg < 1:525035/157‑1 (MQ=255) gtCAATGCCGTCATGAAAAGGCGGATTGGAAATGATCAGATCGAATTTGCCGTCAATA‑TGGGAAAACACGTCACTGGCAACCACCGTGCCTTGCAACCGATTTTCCTG‑TAAAGTGCGCTTTGCGGAAGCCAACGCCAAGGCGTGAATATCCGTCATGAtt > 1:877830/1‑160 (MQ=255) | AACTAAAAGGCTTCGCTAAGCCAGCGGAATTTCGTGCACACTTAACTCATCCGTGCACCGAATAAACCTTAAACTTGTTATTTCTCGCCAGGACGTCATGACTGCCGAAATGTTGATTCAGCCAGTCGGCGTAAGGCAGAAACGCATTTGCCACGATGCGCAGCTCACCGCCCGGTTTCAAATGCCATTTTGCCTGTTTTATCAGTTCGTTAACGGCGGTGTAAGCGGTGTCAATGCCATCATGAAACGGCGGGTTGGAAATGATCAGATCAAATTTGCCGTCAATA‑TGGGAAAACACATCACTGGCAACCACTGTTCCCTGTAGCCGATTTTCCTG‑TAAAGTGCGCTTTGCGGAAGCCAACGCCAAGGCATGAATATCTGTCATGATTAATCGGGTGTTCGGGTTGTGCCGTTGAATATAACTGCCGATCACTCCCGCGCCGCAGCCCACGTCCAACACCCCGCCTGCGATGTTTTT > 1/1986261‑1986738 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |