Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,985,544	G→A	T50I (ACC→ATC)	unknown ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,985,544	0	G	A	100.0%	197.8 / NA	66	T50I (ACC→ATC)	unknown	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (35/31);  total (35/31)

TCTTCATAAAGTTTATATCTGGAATCCTTTTTAATGGCGTCCAAAGCCTCTTCCGTATGCCTACGTTGTACCCAATTAGTGGTAAACGTATAGGTCAATTTGTGATCAAGTTCACTTAGCTGCCATGTTCCCGTGCCCGTGATTTCATACACAAAAAATGGCTTATCAATAGGTTCAATAATGGTGCGGTTATTCACCAACGTGCCATCCCGATTTAAGCGCAGATTATTTACGGTTGTTAAATTGTGTTCAGGGTAAATACTGGTACATTGCCATTCCCCGCTCACTTTGCGTTGGGTAATTTTACCATTGTCTATTAAATCCGTAAGCGTACAGCCGCTCAAAAAAGACAGGGTTAATCCGAGTAAAAATAATTTTTTCATTTGCATCTCCTCTAAAAGTGCGGTGCATTTTTTCAGTG‑TTTTTTATTCCTAATAGGGAATGTCCAAAATACGACAAGGATTTTTTATTGTAATC  >  1/1985310‑1985786                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                    tcttcATAAAGTTTATATCTGGAATCCTTTTTAATGGCGTCCAAAGCCTCTTCCGTATGCCTACGCTGTACCCAATTAGTGGTAAACGTATAGGTCAATTTGTGATCAAGTTCACTTAGCTGCCATGTTCCCGTGCCCGTGATTTCATACACAAAAAATGGCTTATCAATAGGTTCAATAATGGTGCGGTTATTCACCAACGTGCCATCCCGATTTAAGCGCAGATTATTTACGATTGTTAAATTGTGTTc                                                                                                                                                                                                                                     <  1:222264/251‑1 (MQ=255)            tttATATCTGGAATCCTTTTTAATGGCGTCCAAAGCCTCTTCCGTATGCCTACGCTGTACCCAATTAGTGGTAAACGTATAGGTCAATTTGTGATCAAGTTCACTTAGCTGCCATGTTCCCGTGCCCGTGATTTCATACACAAAAAATGGCTTATCAATAGGTTCAATAATGGTGCGGTTATTCACCAACGTGCCATCCCGATTTAAGCGCAGATTATTTACGATTGTTAAATTGTGTTCAGGGTAAATAc                                                                                                                                                                                                                          <  1:816886/251‑1 (MQ=255)            tttATATCTGGAATCCTTTTTAATGGCGTCCAAAGCCTCTTCCGTATGCCTACGCTGTACCCAATTAGTGGTAAACGTATAGGTCAATTTGTGATCAAGTTCACTTAGCTGCCATGTTCCCGTGCCCGTGATTTCATACACAAAAAATGGCTTATCAATAGGTTCAATAATGGTGCGGTTATTCACCAACGTGCCATCCCGATTTAAGCGCAGATTATTTACGATTGTTAAATTGTGTTCAGGGTAAATAc                                                                                                                                                                                                                          <  1:682538/251‑1 (MQ=255)                  tCTGGAATCCTTTTTAATGGCGTCCAAAGCCTCTTCCGTATGCCTACGCTGTACCCAATTAGTGGTAAACGTATAGGTCAATTTGTGATCAAGTTCACTTAGCTGCCATGTTCCCGTGCCCGTGATTTCATACACAAAAAATGGCTTATCAATAGGTTCAATAATGGTGCGGTTATTCACCAACGTGCCATCCCGATTTAAGCGCAGATTATTTACGATTGTTAAATTGTGTTCAGGGTAAATACTGGTAc                                                                                                                                                                                                                    <  1:273462/251‑1 (MQ=255)                   cTGGAATCCTTTTTAATGGCGTCCAAAGCCTCTTCCGTATGCCTACGCTGTACCCAATTAGTGGTAAACGTATAGGTCAATTTGTGATCAAGTTCACTTAGCTGCCATGTTCCCGTGCCCGTGATTTCATACACAAAAAATGGCTTATCAATAGGTTCAATAATGGTGCGGTTATTCACCAACGTGCCATCCCGATTTAAGCGCAGATTATTTACGATTGTTAAATTGTGTTCAGGGTAAATACTGGTACa                                                                                                                                                                                                                   >  1:84429/1‑251 (MQ=255)                        aTCCTTTTTAATGGCGTCCAAAGCCTCTTCCGTATGCCTACGCTGTACCCAATTAGTGGTAAACGTATAGGTCAATTTGTGATCAAGTTCACTTAGCTGCCATGTTCCCGTGCCCGTGATTTCATACACAAAAAATGGCTTATCAATAGGTTCAATAATGGTGCGGTTATTCACCAACGTGCCATCCCGATTTAAGCGCAGATTATTTACGATTGTTAAATTGTGTTCAGGGTAAATACTGGTACATTGcc                                                                                                                                                                                                              <  1:169998/251‑1 (MQ=255)                          ccTTTTTAATGGCGTCCAAAGCCTCTTCCGTATGCCTACGCTGTACCCAATTAGTGGTAAACGTATAGGTCAATTTGTGATCAAGTTCACTTAGCTGCCATGTTCCCGTGCCCGTGATTTCATACACAAAAAATGGCTTATCAATAGGTTCAATAATGGTGCGGTTATTCACCAACGTGCCATCCCGATTTAAGCGCAGATTATTTACGATTGTTAAATTGTGTTCAGGGTAAATACTGGTACATTGCCAt                                                                                                                                                                                                            >  1:403178/1‑251 (MQ=255)                             ttttAATGGCGTCCAAAGCCTCTTCCGTATGCCTACGCTGTACCCAATTAGTGGTAAACGTATAGGTCAATTTGTGATCAAGTTCACTTAGCTGCCATGTTCCCGTGCCCGTGATTTCATACACAAAAAATGGCTTATCAATAGGTTCAATAATGGTGCGGTTATTCACCAACGTGCCATCCCGATTTAAGCGCAGATTATTTACGATTGTTAAATTGTGTTCAGGGTAAATACTGGTACATTGCCATTc                                                                                                                                                                                                          >  1:503125/1‑250 (MQ=255)                             ttttAATGGCGTCCAAAGCCTCTTCCGTATGCCTACGCTGTACCCAATTAGTGGTAAACGTATAGGTCAATTTGTGATCAAGTTCACTTAGCTGCCATGTTCCCGTGCCCGTGATTTCATACACAAAAAATGGCTTATCAATAGGTTCAATAATGGTGCGGTTATTCACCAACGTGCCATCCCGATTTAAGCGCAGATTATTTACGATTGTTAAATTGTGTTCAGGGTAAATACTGGTACATTGCCATTc                                                                                                                                                                                                          >  1:767665/1‑250 (MQ=255)                                           aaaGCCTCTTCCGTATGCCTACGCTGTACCCAATTAGTGGTAAACGTATAGGTCAATTTGTGATCAAGTTCACTTAGCTGCCATGTTCCCGTGCCCGTGATTTCATACACAAAAAATGGCTTATCAATAGGTTCAATAATGGTGCGGTTATTCACCAACGTGCCATCCCGATTTAAGCGCAGATTATTTACGATTGTTAAATTGTGTTCAGGGTAAATACTGGTACATTGCCATTCCCCGCTCACTTTGCg                                                                                                                                                                                           <  1:556074/251‑1 (MQ=255)                                                    tCCGTATGCCTACGCTGTACCCAATTAGTGGTAAACGTATAGGTCAATTTGTGATCAAGTTCACTTAGCTGCCATGTTCCCGTGCCCGTGATTTCATACACAAAAAATGGCTTATCAATAGGTTCAATAATGGTGCGGTTATTCACCAACGTGCCATCCCGATTTAAGCGCAGATTATTTACGATTGTTAAATTGTGTTCAGGGTAAATACTGGTACATTGcc                                                                                                                                                                                                              <  1:119178/223‑1 (MQ=255)                                                     ccGTATGCCTACGCTGTACCCAATTAGTGGTAAACGTATAGGTCAATTTGTGGTCAAGTTCACTTAGCTGCCATGTTCCCGTGCCCGTGATTTCATACACAAAAAATGGCTTATCAATAGGTTCAATAATGGTGCGGTTATTCACCAACGTGCCATCCCGATTTAAGCGCAGATTATTTACGATTGTTAAATTGTGTTc                                                                                                                                                                                                                                     <  1:148089/199‑1 (MQ=255)                                                          tGCCTACGCTGTACCCAATTAGTGGTAAACGTATAGGTCAATTTGTGTTCAAGTTCACTTAGCTGCCATGTTCCCGTGCCCGTGATTTCATACACAAAAAATGGCTTATCAATAGGTTCAATAATGGTGCGGTTATTCACCAACGTGCCATCCCGATTTAAGCGCAGATTATTTACGATTGTTAAATTGTGTTCAGGGTAAATACTGGTACATTGCCATTCCCCGCTCACTTTGCGTTGGGTAATTTTAc                                                                                                                                                                             >  1:398795/1‑250 (MQ=255)                                                          tGCCTACGCTGTACCCAATTAGTGGTAAACGTATAGGTCAATTTGTGATCAAGTTCACTTAGCTGCCATGTTCCCGTGCCCGTGATTTCATACACAAAAAATGGCTTATCAATAGGTTCAATAATGGTGCGGTTATTCACCAACGTGCCATCCCGATTTAAGCGCAGATTATTTACGATTGTTAAATTGTGTTCAGGGTAAATACTGGTACATTGCCATTCCCCGCTCACTTTGCGTTGGGTAATTTTAc                                                                                                                                                                             >  1:811353/1‑250 (MQ=255)                                                           gCCTACGCTGTACCCAATTAGTGGTAAACGTATAGGTCAATTTGTGATCAAGTTCACTTAGCTGCCATGTTCCCGTGCCCGTGATTTCATACACAAAAAATGGCTTATCAATAGGTTCAATAATGGTGCGGTTATTCACCAACGTGCCATCCCGATTTAAGCGCAGATTATTTACGATTGTTAAATTGTGTTCAGGGTAAATACTGGTACATTGCCATTCCCCGCTCACTTTGCGTTGGGTAATTTTACCa                                                                                                                                                                           >  1:13717/1‑251 (MQ=255)                                                                      aCCCAATTAGTGGTAAACGTATAGGTCAATTTGTGATCAAGTTCACTTAGCTGCCATGTTCCCGTGCCCGTGATTTCATACACAAAAAATGGCTTATCAATAGGTTCAATAATGGTGCGGTTATTCACCAACGTGCCATCCCGATTTAAGCGCAGATTATTTACGATTGTTAAATTGTGTTCAGGGTAAATACTGGTACATTGCCATTCCCCGCTCACTTTGCGTTGGGTAATTTTACCATTGTCTATTaa                                                                                                                                                                >  1:749249/1‑251 (MQ=255)                                                                       cccAATTAGTGGTAAACGTATAGGTCAATTTGTGATCAAGTTCACTTAGCTGCCATGTTCCCGTGCCCGTGATTTCATACACAAAAAATGGCTTATCAATAGGTTCAATAATGGTGCGGTTATTCACCAACGTGCCATCCCGATTTAAGCGCAGATTATTTACGATTGTTAAATtg                                                                                                                                                                                                                                          <  1:705279/176‑1 (MQ=255)                                                                       cccAATTAGTGGTAAACGTATAGGTCAATTTGTGATCAAGTTCACTTAGCTGCCATGTTCCCGTGCCCGTGATTTCATACACAAAAAATGGCTTATCAATAGGTTCAATAATGGTGCGGTTATTCACCAACGTGCCATCCCGATTTAAGCGCAGATTATTTACGATTGTTAAATTGTGTTCAGGGTAAATACTGGTACATTGCCATTCCCCGCTCACTTTGCGTTGGGTAATTTTACCATTGTCTATTaaa                                                                                                                                                               >  1:317148/1‑251 (MQ=255)                                                                         cAATTAGTGGTAAACGTATAGGTCAATTTGTGATCAAGTTCACTTAGCTGCCATGTTCCCGTGCCCGTGATTTCATACACAAAAAATGGCTTATCAATAGGTTCAATAATGGTGCGGTTATTCACCAACGTGCCATCCCGATTTAAGCGCAGATTATTTACGATTGTTAAATTGTGTTCAGGGTAAATACTGGTACATTGCCATTCCCCGCTCACTTTGCGTTGGGTAATTTTACCATTGTCTATTAAATc                                                                                                                                                             <  1:699776/251‑1 (MQ=255)                                                                                   tAAACGTATAGGTCAATTTGTGATCAAGTTCACTTAGCTGCCATGTTCCCGTGCCCGTGATTTCATACACAAAAAATGGCTTATCAATAGGTTCAATAATGGTGCGGTTATTCACCAACGTGCCATCCCGATTTAAGCGCAGATTATTTACGATTGTTAAATTGTGTTCAGGGTAAATACTGGTACATTGCCATTCCCCGCTCACTTTGCGTTGGGTAATTTTACCATTGTCTATTAAATCCGTAAGCGt                                                                                                                                                    <  1:16197/250‑1 (MQ=255)                                                                                       cGTATAGGTCAATTTGTGATCAAGTTCACTTAGCTGCCATGTTCCCGTGCCCGTGATTTCATACACAAAAAATGGCTTATCAATAGGTTCAATAATGGTGCGGTTATTCACCAACGTGCCATCCCGATTTAAGCGCAGATTATTTACGATTGTTAAATTGTGTTCAGGGTAAATACTGGTACATTGCCATTCCCCGCTCACTTTGCGTTGGGTAATTTTACCATTGTCTATTAAATc                                                                                                                                                             <  1:792601/237‑1 (MQ=255)                                                                                       cGTATAGGTCAATTTGTGATCAAGTTCACTTAGCTGCCATGTTCCCGTGCCCGTGATTTCATACACAAAAAATGGCTTATCAATAGGTTCAATAATGGTGCGGTTATTCACCAACGTGCCATCCCGATTTAAGCGCAGATTATTTACGATTGTTAAATTGTGTTCAGGGTAAATACTGGTACATTGCCATTCCCCGCTCACTTTGCGTTGGGTAATTTTACCATTGTCTATTAAATCCGTAAGCGTACAGc                                                                                                                                               <  1:9343/251‑1 (MQ=255)                                                                                       cGTATAGGTCAATTTGTGATCAAGTTCACTTAGCTGCCATGTTCCCGTGCCCGTGATTTCATACACAAAAAATGGCTTATCAATAGGTTCAATAATGGTGCGGTTATTCACCAACGTGCCATCCCGATTTAAGCGCAGATTATTTACGATTGTTAAATTGTGTTCAGGGTAAATACTGGTACATTGCCATTCCCCGCTCACTTTGCGTTGGGTAATTTTACCATTGTCTATTAAATCCGTAAGCGTACAGc                                                                                                                                               <  1:455965/251‑1 (MQ=255)                                                                                       cGTATAGGTCAATTTGTGATCAAGTTCACTTAGCTGCCATGTTCCCGTGCCCGTGATTTCATACACAAAAAATGGCTTATCAATAGGTTCAATAATGGTGCGGTTATTCACCAACGTGCCATCCCGATTTAAGCGCAGATTATTTACGATTGTTAAATTGTGTTCAGGGTAAATACTGGTACATTGCCATTCCCCGCTCACTTTGCGTTGGGTAATTTTACCATTGTCTATTAAATCCGTAAGCGTACAGc                                                                                                                                               <  1:404295/251‑1 (MQ=255)                                                                                        gTATAGGTCAATTTGTGATCAAGTTCACTTAGCTGCCATGTTCCCGTGCCCGTGATTTCATACACAAAAAATGGCTTATCAATAGGTTCAATAATGGTGCGGTTATTCACCAACGTGCCATCCCGATTTAAGCGCAGATTATTTACGATTGTTAAATTGTGTTCAGGGTAAATACTGGTACATTGCCATTCCCCGCTCACTTTGCGTTGGGTAATTTTACCATTGTCTATTAAATCCGTAAGCGTACAGc                                                                                                                                               >  1:526994/1‑250 (MQ=255)                                                                                        gTATAGGTCAATTTGTGATCAAGTTCACTTAGCTGCCATGTTCCCGTGCCCGTGATTTCATACACAAAAAATGGCTTATCAATAGGTTCAATAATGGTGCGGTTATTCACCAACGTGCCATCCCGATTTAAGCGCAGATTATTTACGATTGTTAAATTGTGTTCAGGGTAAATACTGGTACATTGCCATTCCCCGCTCACTTTGCGTTGGGTAATTTTACCATTGTCTATTAAATCCGTAAGCGTACAGc                                                                                                                                               >  1:94932/1‑250 (MQ=255)                                                                                        gTATAGGTCAATTTGTGATCAAGTTCACTTAGCTGCCATGTTCCCGTGCCCGTGATTTCATACACAAAAAATGGCTTATCAATAGGTTCAATAATGGTGCAGTTATTCACCAACGTGCCATCCCGATTTAAGCGCAGATTATTTACGATTGTTAAATTGTGTTCAGGGTAAATACTGGTACATTGCCATTCCCCGCTCACTTTGCGTTGGGTAATTTTACCATTGTCTATTAAATCCGTAAGCGTACAGc                                                                                                                                               >  1:106576/1‑250 (MQ=255)                                                                                                      gtgATCAAGTTCACTTAGCTGCCATGTTCCCGTGCCCGTGATTTCATACACAAAAAATGGCTTATCAATAGGTTCAATAATGGTGCGGTTATTCACCAACGTGCCATCCCGATTTAAGCGCAGATTATTTACGATTGTTAAATtg                                                                                                                                                                                                                                          >  1:521776/1‑145 (MQ=255)                                                                                                               ttCACTTAGCTGCCATGTTCCCGTGCCCGTGATTTCATACACAAAAAATGGCTTATCAATAGGTTCAATAATGGTGCGGTTATTCACCAACGTGCCATCCCGATTTAAGCGCAGATTATTTACGATTGTTAAATTGTGTTCAGGGTAAATACTGGTACATTGCCATTCCCCGCTCACTTTGCGTTGGGTAATTTTACCATTGTCTATTAAATCCGTAAGCGTACAGCCGCTCAAAAAAGACAGGGTTAATc                                                                                                                       <  1:724482/251‑1 (MQ=255)                                                                                                                  aCTTAGCTGCCATGTTCCCGTGCCCGTGATTTCATACACAAAAAATGGCTTATCAATAGGTTCAATAATGGTGCGGTTATTCACCAACGTGCCATCCCGATTTAAGCGCAGATTATTTACGATTGTTAAATTGTGTTCAGGGTAAATACTGGTACATTGCCATTCCCCGCTCACTTTGCGTTGGGTAATTTTACCATTGTCTATTaa                                                                                                                                                                >  1:839443/1‑207 (MQ=255)                                                                                                                          gCCATGTTCCCGTGCCCGTGATTTCATACACAAAAAATGGCTTATCAATAGGTTCAATAATGGTGCGGTTATTCACCAACGTGCCATCCCGATTTAAGCGCAGATTATTTACGATTGTTAAATTGTGTTCAGGGTAAATACTGGTACATTGCCATTCCCCGCTCACTTTGCGTTGGGTAATTTTACCATTGTCTATTAAATCCGTAAg                                                                                                                                                       <  1:850900/208‑1 (MQ=255)                                                                                                                          gCCATGTTCCCGTGCCCGTGATTTCATACACAAAAAATGGCTTATCAATAGGTTCAATAATGGTGCGGTTATTCACCAACGTGCCATCCCGATTTAAGCGCAGATTATTTACGATTGTTAAATTGTGTTCAGGGTAAATACTGGTACATTGCCATTCCCCGCTCACTTTGCGTTGGGTAATTTTACCATTGTCTATTAAATCCGTAAg                                                                                                                                                       <  1:340194/208‑1 (MQ=255)                                                                                                                             aTGTTCCCGTGCCCGTGATTTCATACACAAAAAATGGCTTATCAATAGGTTCAATAATGGTGCGGTTATTCACCAACGTGCCATCCCGATTTAAGCGCAGATTATTTACGATTGTTAAATTGTGTTCAGGGTAAATACTGGTAc                                                                                                                                                                                                                    <  1:742737/144‑1 (MQ=255)                                                                                                                             aTGTTCCCGTGCCCGTGATTTCATACACAAAAAATGGCTTATCAATAGGTTCAATAATGGTGCGGTTATTCACCAACGTGCCATCCCGATTTAAGCGCAGATTATTTACGATTGTTAAATTGTGTTCAGGGTAAATACTGGTACATTGCCATTCCCCGCTCACTTTGCGTTGGGTAATTTTACCATTGTCTATTAAATCCGTAAGCGTACAGCCGCTCAAAAAAGACAGGGTTAATCCGAGTaaaaa                                                                                                             <  1:727551/247‑1 (MQ=255)                                                                                                                               gTTCCCGTGCCCGTGATTTCATACACAAAAAATGGCTTATCAATAGGTTCAATAATGGTGCGGTTATTCACCAACGTGCCATCCCGATTTAAGCGCAGATTATTTACGATTGTTAAATTGTGTTCAGGGTAAATACTGGTACATTGCCATTCCCCGCTCACTTTGCGTTGGGTAATTTTACCATTGTCTATTAAATCCGTAAGCGTACAGCCGCTCAAAAAAGACAGGGTTAATc                                                                                                                       >  1:334586/1‑235 (MQ=255)                                                                                                                               gTTCCCGTGCCCGTGATTTCATACACAAAAAATGGCTTATCAATAGGTTCAATAATGGTGCGGTTATTCACCAACGTGCCATCCCGATTTAAGCGCAGATTATTTACGATTGTTAAATTGTGTTCAGGGTAAATACTGGTACATTGCCATTCCCCGCTCACTTTGCGTTGGGTAATTTTACCATTGTCTATTAAATCCGTAAGCGTACAGCCGCTCAAAAAAGACAGGGTTAATCCGAGTAAAAATAAttt                                                                                                       >  1:547939/1‑251 (MQ=255)                                                                                                                                        cccgtgATTTCATACACAAAAAATGGCTTATCAATAGGTTCAATAATGGTGCGGTTATTCACCAACGTGCCATCCCGATTTAAGCGCAGATTATTTACGATTGTTAAATTGTGTTCAGGGTAAATACTGGTACATTGCCATTCCCCGCTCACTTTGCGTTGGGTAATTTTACCATTGTCTATTAAATCCGTAAGCGTACAGCCGCTCAAAAAAGACAGGGTTAATCCGAGTAAAAATAATTTTTTCATTTg                                                                                              >  1:288012/1‑251 (MQ=255)                                                                                                                                                     acacAAAAAATGGCTTATCAATAGGTTCAATAATGGTGCGGTTATTCACCAACGTGCCATCCCGATTTAAGCGCAGATTATTTACGATTGTTAAATTGTGTTCAGGGTAAATACTGGTACATTGCCATTCCCCGCTCACTTTGCGTTGGGTAATTTTACCATTGTCTATTAAATCCGTAAGCGTACAGCCGCTCAAAAAAGACAGGGTTAATCCGAGTAAAAATAATTTTTTCATTTGCATCTCCTCTaaa                                                                                 >  1:376141/1‑251 (MQ=255)                                                                                                                                                       acaAAAAATGGCTTATCAATAGGTTCAATAATGGTGCGGTTATTCACCAACGTGCCATCCCGATTTAAGCGCAGATTATTTACGATTGTTAAATTGTGTTCAGGGTAAATACTGGTACATTGCCATTCCCCGCTCACTTTGCGTTGGGTAATTTTACCATTGTCTATTAAATCCGTAAGCGTACAGCCGCTCAAAAAAGACAGGGTTAATCCGAGTAAAAATAATTTTTTCATTTGCATCTCCTCTAAAAg                                                                               <  1:348254/251‑1 (MQ=255)                                                                                                                                                          aaaaaTGGCTTATCAATAGGTTCAATAATGGTGCGGTTATTCACCAACGTGCCATCCCGATTTAAGCGCAGATTATTTACGATTGTTAAATTGTGTTCAGGGTAAATACTGGTACATTGCCATTCCCCGCTCACTTTGCGTTGGGTAATTTTACCATTGTCTATTAAATCCGTAAGCGTACAGCCGCTCAAAAAAGACAGGGTTAATc                                                                                                                       >  1:397270/1‑208 (MQ=255)                                                                                                                                                          aaaaaTGGCTTATCAATAGGTTCAATAATGGTGCGGTTATTCACCAACGTGCCATCCCGATTTAAGCGCAGATTATTTACGATTGTTAAATTGTGTTCAGGGTAAATACTGGTACATTGCCATTCCCCGCTCACTTTGCGTTGGGTAATTTTACCATTGTCTATTAAATCCGTAAGCGTACAGCCGCTCAAAAAAGACAGGGTTAATc                                                                                                                       >  1:127765/1‑208 (MQ=255)                                                                                                                                                           aaaaTGGCTTATCAATAGGTTCAATAATGGTGCGGTTATTCACCAACGTGCCATCCCGATTTAAGCGCAGATTATTTACGATTGTTAAATTGTGTTCAGGGTAAATACTGGTACATTGCCATTCCCCGCTCACTTTGCGTT‑GGTAATTTTACCATTGTCTATTAAATCCGTAAGCGTACAGCCGCTCAAAAAAGACAGGGTTAATCCGAGTAAAAATAATTTTTTCATTTGCATCTCCTCTAAAAGTGCgg                                                                          >  1:147927/1‑251 (MQ=255)                                                                                                                                                                    tATCAATAGGTTCAATAATGGTGCGGTTATTCACCAACGTGCCATCCCGATTTAAGCGCAGATTATTTACGATTGTTAAATTGTGTTCAGGGTAAATACTGGTACATTGCCATTccc                                                                                                                                                                                                        >  1:228589/1‑117 (MQ=255)                                                                                                                                                                      tCAATAGGTTCAATAATGGTGCGGTTATTCACCAACGTGCCATCCCGATTTAAGCGCAGATTATTTACGATTGTTAAATTGTGTTCAGGGTAAATACTGGTACATTGCCATTCCCCGCTCACTTTGCGTTGGGTAATTTTACCATTGTCTATTAAATCCGTAAGCGTACAGCCGCTCAAAAAAGACAGGGTTAATCCGAGTAAAAATAATTTTTTCATTTGCATCTCCTCTAAAAGTGCGGTGCAtttttt                                                                >  1:520796/1‑251 (MQ=255)                                                                                                                                                                             gTTCAATAATGGTGCGGTTATTCACCAACGTGCCATCCCGATTTAAGCGCAGATTATTTACGATTGTTAAATTGTGTTCAGGGTAAATACTGGTACATTGCCATTCCCCGCTCACTTTGCGTTGGGTAATTTTACCATTGTCTATTAAATCCGTAAGCGTACAGCCGCTCAAAAAAGACAGGGTTAATc                                                                                                                       >  1:552996/1‑189 (MQ=255)                                                                                                                                                                             gTTCAATAATGGTGCGGTTATTCACCAACGTGCCATCCCGATTTAAGCGCAGATTATTTACGATTGTTAAATTGTGTTCAGGGTAAATACTGGTACATTGCCATTCCCCGCTCACTTTGCGTTGGGTAATTTTACCATTGTCTATTAAATCCGTAAGCGTACAGCCGCTCAAAAAAGACAGGGTTAATCCGAGTAAAAATAATTTTTTCATTTGCAt                                                                                           <  1:128607/217‑1 (MQ=255)                                                                                                                                                                             gTTCAATAATGGTGCGGTTATTCACCAACGTGCCATCCCGATTTAAGCGCAGATTATTTACGATTGTTAAATTGTGTTCAGGGTAAATACTGGTACATTGCCATTCCCCGCTCACTTTGCGTTGGGTAATTTTACCATTGTCTATTAAATCCGTAAGCGTACAGCCGCTCAAAAAAGACAGGGTTAATCCGAGTAAAAATAATTTTTTCATTTGCATCTCCTCTAAAAGTGCGGTGCATTTTTTCAGTG‑tt                                                        >  1:817268/1‑251 (MQ=255)                                                                                                                                                                             gTTCAATAATGGTGCGGTTATTCACCAACGTGCCATCCCGATTTAAGCGCAGATTATTTACGATTGTTAAATTGTGTTCAGGGTAAATACTGGTACATTGCCATTCCCCGCTCACTTTGCGTTGGGTAATTTTACCATTGTCTATTAAATCCGTAAGCGTACAGCCGCTCAAAAAAGACAGGGTTAATCCGAGTAAAAATAATTTTTTCATTTGCATCTCCTCTAAAAGTGCGGTGCATTTTTTCAGTG‑tt                                                        >  1:255658/1‑251 (MQ=255)                                                                                                                                                                             gTTCAATAATGGTGCGGTTATTCACCAACGTGCCATCCCGATTTAAGCGCAGATTATTTACGATTGTTAAATTGTGTTCAGGGTAAATACTGGTACATTGCCATTCCCCGCTCACTTTGCGTTGGGTAATTTTACCATTGTCTATTAAATCCGTAAGCGTACAGCCGCTCAAAAAAGACAGGGTTAATCCGAGTAAAAATAATTTTTTCATTTGCATCTCCTCTAAAAGTGCGGTGCATTTTTTCAGTG‑tt                                                        >  1:258263/1‑251 (MQ=255)                                                                                                                                                                                cAATAATGGTGCGGTTATTCACCAACGTGCCATCCCGATTTAAGCGCAGATTATTTACGATTGTTAAATTGTGTTCAGGGTAAATACTGGTACATTGCCATTCCCCGCTCACTTTGCGTTGGGTAATTTTACCATTGTCTATTAAATCCGTAAGCGTACAGCCGCTCaaaaa                                                                                                                                     <  1:5825/172‑1 (MQ=255)                                                                                                                                                                                cAATAATGGTGCGGTTATTCACCAACGTGCCATCCCGATTTAAGCGCAGATTATTTACGATTGTTAAATTGTGTTCAGGGTAAATACTGGTACATTGCCATTCCCCGCTCACTTTGCGTTGGGTAATTTTACCATTGTCTATTAAATCCGTAAGCGTACAGCCGCTCAAAAAAGACAGGGTTAATc                                                                                                                       <  1:820764/186‑1 (MQ=255)                                                                                                                                                                                  ataatGGTGCGGTTATTCACCAACGTGCCATCCCGATTTAAGCGCAGATTATTTACGATTGTTAAATTGTGTTCAGGGTAAATACTGGTACATTGCCATTCCCCGCTCACTTTGCGTTGGGTAATTTTACCATTGTCTATTAAATCCGTAAGCGTACAGCCGCTCAAAAAAGACAGGGt                                                                                                                            <  1:729524/179‑1 (MQ=255)                                                                                                                                                                                  ataatGGTGCGGTTATTCACCAACGTGCCATCCCGATTTAAGCGCAGATTATTTACGATTGTTAAATTGTGTTCAGGGTAAATACTGGTACATTGCCATTCCCCGCTCACTTTGCGTTGGGTAATTTTACCATTGTCTATTAAATCCGTAAGCGTACAGCCGCTCAAAAAAGACAGGGTTAATCCGAGTAAAAATAATTTTTTCATTTGCATCTCCTCTAAAAGTGCGGTGCATTTTTTCAGTG‑ttttttt                                                   >  1:377439/1‑250 (MQ=255)                                                                                                                                                                                             gTTATTCACCAACGTGCCATCCCGATTTAAGCGCAGATTATTTACGATTGTTAAATTGTGTTCAGGGTAAATACTGGTACATTGCCATTCCCCGCTCACTTTGCGTTGGGTAATTTTACCATTGTCTATTAAATCCGTAAGCGTACAGCCGCTCAAAAAAGACAGGGTTAATCCGAGTAAAAATAATTTTTTCATTTGCATCTCCTCTAAAAGTGCGGTGCATTTTTTCAGTGTTTTTTTATTCCTAATAg                                         >  1:369590/1‑251 (MQ=255)                                                                                                                                                                                                aTTCACCAACGTGCCATCCCGATTTAAGCGCAGATTATTTACGATTGTTAAATTGTGTTc                                                                                                                                                                                                                                     >  1:41335/1‑60 (MQ=255)                                                                                                                                                                                                aTTCACCAACGTGCCATCCCGATTTAAGCGCAGATTATTTACGATTGTTAAATTGTGTTCAGGGTAAATACTGGTACATTGCCATTCTCCGCTCACTTTGCGTTGGGTATTTTTACCATTGTCTATTAAATCCGTAAGCGTACAGCCGCTCAAAAAAGACAGGGTTAATCCGAGTAAAAATAATTTTTTCATTTGCATCTCCTCTAAAAGTGCGGTGCATTTTTTCAGTGTTTTTTTATTCCTAATAGGGa                                      >  1:55503/1‑251 (MQ=255)                                                                                                                                                                                                     ccAACGTGCCATCCCGATTTAAGCGCAGATTATTTACGATTGTTAAATTGTGTTCAGGGTAAATACTGGTACATTGCCATTCCCCGCTCACTTTGCGTTGGGTAATTTTACCAt                                                                                                                                                                          <  1:534099/114‑1 (MQ=255)                                                                                                                                                                                                          gTGCCATCCCGATTTAAGCGCAGATTATTTACGATTGTTAAATTGTGTTCAGGGTAAATACTGGTACATTGCCATTCCCCGCTCACTTTGCGTTGGGTAATTTTACCATTGTCTATTAAATCCGTAAGCGTACAGCCGCTCAAAAAAGACAGGGTTAATCCGAGTAAAAATAATTTTTTCATTTGCATCTCCTCTAAAAGTGCGGTGCATTTTTTCAGTGTTTTTTTATTCCTAATAGGGAATGTCCaaaa                            >  1:559176/1‑251 (MQ=255)                                                                                                                                                                                                               aTCCCGATTTAAGCGCAGATTATTTACGATTGTTAAATTGTGTTCAGGGTAAATACTGGTACATTGCCATTCCCCGCTCACTTTGCGTTGGGTAATTTTACCATTGTCTATTAAATCCGTAAGCGTACAGCCGCTCAAAAAAGACAGGGTTAATc                                                                                                                       >  1:777237/1‑155 (MQ=255)                                                                                                                                                                                                               aTCCCGATTTAAGCGCAGATTATTTACGATTGTTAAATTGTGTTCAGGGTAAATACTGGTACATTGCCATTCCCCGCTCACTTTGCGTTGGGTAATTTTACCATTGTCTATTAAATCCGTAAGCGTACAGCCGCTCAAAAAAGACAGGGTTAATCCGAGTAAAAATAATTTTTTCATTTGCATCTCCTCTAAAAGTGCGGTGCATTTTTTCAGTGTTTTTTTATTCCTAATAggg                                       >  1:459094/1‑235 (MQ=255)                                                                                                                                                                                                               aTCCCGATTTAAGCGCAGATTATTTACGATTGTTAAATTGTGTTCAGGGTAAATACTGGTACATTGCCATTCCCCGCTCACTTTGCGTTGGGTAATTTTACCATTGTCTATTAAATCCGTAAGCGTACAGCCGCTCAAAAAAGACAGGGTTAATCCGAGTAAAAATAATTTTTTCATTTGCATCTCCTCTAAAAGTGCGGTGCATTTTTTCAGTGTTTTTTTATTCCTAATAGGGAATGTCCAAAATACGa                       >  1:865190/1‑251 (MQ=255)                                                                                                                                                                                                                     aTTTAAGCGCAGATTATTTACGATTGTTAAATTGTGTTCAGGGTAAATACTGGTACATTGCCATTccc                                                                                                                                                                                                        <  1:574053/68‑1 (MQ=255)                                                                                                                                                                                                                            cgcAGATTATTTACGATTGTTAAATTGTGTTCAGGGTAAATACTGGTACATTGCCATTCCCCGCTCACTTTGCGTTGGGTAATTTTACCATTGTCTATTAAATCCGTAAGCGTACAGCCGCTCAAAAAAGACAGGGTTAATCCGAGTAAAAATAATTTTTTCATTTGCATCTCCTCTAAAAGTGCGGTGCATTTTTTCAGTGTTTTTTTATTCCTAATAGGGAATGTCCAAAATACGACAAGGAtttttt           <  1:655858/250‑1 (MQ=255)                                                                                                                                                                                                                                  ttattTACGATTGTTAAATTGTGTTCAGGGTAAATACTGGTACATTGCCATTCCCCGCTCACTTTGCGTTGGGTAATTTTACCATTGTCTATTAAATCCGTAAGCGTACAGCCGCTCAAAAAAGACAGGGTTAATCCGAGTAAAAATAATTTTTTCATTTGCATCTCCTCTAAAAGTGCGGTGCATTTTTTCAGTGTTTTTTTATTCCTAATAGGGa                                      <  1:532604/217‑1 (MQ=255)                                                                                                                                                                                                                                  ttattTACGATTGTTAAATTGTGTTCAGGGTAAATACTGGTACATTGCCATTCCCCGCTCACTTTGCGTTGGGTAATTTTACCATTGTCTATTAAATCCGTAAGCGTACAGCCGCTCAAAAAAGACAGGGTTAATCCGAGTAAAAATAATTTTTTCATTTGCATCTCCTCTAAAAGTGCGGTGCATTTTTTCAGTGTTTTTTTATTCCTAATAGGGAATg                                   <  1:703080/220‑1 (MQ=255)                                                                                                                                                                                                                                    attTACGATTGTTAAATTGTGTTCAGGGTAAATACTGGTACATTGCCATTCCCCGCTCACTTTGCGTTGGGTAATTTTACCATTGTCTATTAAATCCGTAAGCGTACAGCCGCTCAAAAAAGACAGGGTTAATCCGAGTAAAAATAATTTTTTCATTTGCATCTCCTCTAAAAGTGCGGTGCATTTTTTCAGTGTTTTTTTATTCCTAATAGGGAATGTCCAAAATACGACAAGGATTTTTTATTGTAATc  <  1:496769/251‑1 (MQ=255)                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                    TCTTCATAAAGTTTATATCTGGAATCCTTTTTAATGGCGTCCAAAGCCTCTTCCGTATGCCTACGTTGTACCCAATTAGTGGTAAACGTATAGGTCAATTTGTGATCAAGTTCACTTAGCTGCCATGTTCCCGTGCCCGTGATTTCATACACAAAAAATGGCTTATCAATAGGTTCAATAATGGTGCGGTTATTCACCAACGTGCCATCCCGATTTAAGCGCAGATTATTTACGGTTGTTAAATTGTGTTCAGGGTAAATACTGGTACATTGCCATTCCCCGCTCACTTTGCGTTGGGTAATTTTACCATTGTCTATTAAATCCGTAAGCGTACAGCCGCTCAAAAAAGACAGGGTTAATCCGAGTAAAAATAATTTTTTCATTTGCATCTCCTCTAAAAGTGCGGTGCATTTTTTCAGTG‑TTTTTTATTCCTAATAGGGAATGTCCAAAATACGACAAGGATTTTTTATTGTAATC  >  1/1985310‑1985786

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑