Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,980,457	G→C	T557R (ACA→AGA)	unknown ←	RTX toxins determinant A and related Ca2+‑binding proteins

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,980,457	0	G	C	100.0%	127.3 / NA	43	T557R (ACA→AGA)	unknown	RTX toxins determinant A and related Ca2+‑binding proteins
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (21/22);  total (21/22)

CATCATAACCGTCTCCAGCATTAACTATTGTTGAACCGGATCCGACAAAAACATAATCATCTTTAGCACCCAAATTAGCAATTAATCTTGCTTCTAGAACTTTATTATCACGTGTAACGGCATGTTGAATTAAGTTAGAAAAATCATATACACCATTAGAATTAGGAACACCTTTTACCTTCCAATCGGTACGTCCTTTTACTTTTAATTCAGTAATAAATTCATATTTACCTGACTGACGTGTTTTCCGTTCTTCCTTACCTGCGGTTAACAACGGATTTAAAAAAGTTAGAGTGGTAGAACCTTTTATACCCGAAAGATCAATATTACCTTTGATTGGATCTAAAATCTGTTTAGTGAATTTATCGCTATGCTTTGCAAGCTCCTCACCCTTTTTCAAATAATCCACATAAGCTTTACCACTTTGAATACGATCTCCATTACGAGTGATACCTGCTAATTCACCAATGCGCTGATCCCAAC  >  1/1980215‑1980697                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                 catcatAACCGTCTCCAGCATTAACTATTGTTGAACCGGATCCGACAAAAACATAATCATCTTTAGCACCCAAATTAGCAATTAATCTTGCTTCTAGAACTTTATTATCACGTGTAACGGCATGTTGAATTAAGTTAGAAAAATCATATACACCATTAGAATTAGGAACACCTTTTACCTTCCAATCGGTACGTCCTTTTACTTTTAATTCAGTAATAAATTCATATTTACCTGACTGACGTCTTTTCCg                                                                                                                                                                                                                                           >  1:164487/1‑250 (MQ=255)                                             aCAAAAACATAATCATCTTTAGCACCCAAATTAGCAATTAATCTTGCTTCTAGAACTTTATTATCACGTGTAACGGCATGTTGAATTAAGTTAGAAAAATCATATACACCATTAGAATTAGGAACACCTTTTACCTTCCAATCGGTACGTCCTTTTACTTTTAATTCAGTAATAAATTCATATTTACCTGACTGACGTCTTTTCCGTTCTTCCTTACCTGCGGTTAACAACGGATTTAAAAAAGTTagag                                                                                                                                                                                               <  1:727381/250‑1 (MQ=255)                                             aCAAAAACATAATCATCTTTAGCACCCAAATTAGCAATTAATCTTGCTTCTAGAACTTTATTATCACGTGTAACGGCATGTTGAATTAAGTTAGAAAAATCATATACACCATTAGAATTAGGAACACCTTTTACCTTCCAATCGGTACGTCCTTTTACTTTTAATTCAGTAATAAATTCATATTTACCTGACTGACGTCTTTTCCGTTCTTCCTTACCTGCGGTTAACAACGGATTTAAAAAAGTTAGAGt                                                                                                                                                                                              <  1:220181/251‑1 (MQ=255)                                                            tcTTTAGCACCCAAATTAGCAATTAATCTTGCTTCTAGAACTTTATTATCACGTGTAACGGCATGTTGAATTAAGTTAGAAAAATCATATACACCATTAGAATTAGGAACACCTTTTACCTTCCAATCGGTACGTCCTTTTACTTTTAATTCAGTAATAAATTCATATTTACCTGACTGACGTCTTTTCCGTTCTTCCTTACCTGCGGTTAACAACGGATTTAAAAAAGTTAGAGTGGTAGAACCTTTtat                                                                                                                                                                               >  1:570113/1‑251 (MQ=255)                                                                    aCCCAAATTAGCAATTAATCTTGCTTCTAGAACTTTATTATCACGTGTAACGGCATGTTGAATTAAGTTAGAAAAATCATATACACCATTAGAATTAGGAACACCTTTTACCTTCCAATCGGTACGTCCTTTTACTTTTAATTCAGTAATAAATTCATATTTACCTGACTGACGTCTTTTCCGTTCTTCCTTAcc                                                                                                                                                                                                                               <  1:595067/195‑1 (MQ=255)                                                                                          gCTTCTAGAACTTTATTATCACGTGTAACGGCATGTTGAATTAAGTTAGAAAAATCATATACACCATTAGAATTAGGAACACCTTTTACCTTCCAATCGGTACGTCCTTTTACTTTTAATTCAGTAATAAATTCATATTTACCTGACTGACGTCTTTTCCGTTCTTCCTTACCTGCGGTTAACAACGGATTTAAAAAAGTTAGAGTGGTAGAACCTTTTATAc                                                                                                                                                                             <  1:336632/223‑1 (MQ=255)                                                                                            ttCTAGAACTTTATTATCACGTGTAACGGCATGTTGAATTAAGTTAGAAAAATCATATACACCATTAGAATTAGGAACACCTTTTACCTTCCAATCGGTACGTCCTTTTACTTTTAATTCAGTAATAAATTCATATTTACCTGACTGACGTCTTTTCCGTTCTTCCTTAcc                                                                                                                                                                                                                               <  1:900457/171‑1 (MQ=255)                                                                                                      ttattaTCACGTGTAACGGCATGTTGAATTAAGTTAGAAAAATCATATACACCATTAGAATTAGGAACACCTTTTACCTTCCAATCGGTACGTCCTTTTACTTTTAATTCAGTAATAAATTCATATTTACCTGACTGACGTCTTTTCCGTTCTTCCTTACCTGCGGTTAACAACGGATTTAAAAAAGTTAGAGTGGTAg                                                                                                                                                                                         <  1:878669/199‑1 (MQ=255)                                                                                                      ttattaTCACGTGTAACGGCATGTTGAATTAAGTTAGAAAAATCATATACACCATTAGAATTAGGAACACCTTTTACCTTCCAATCGGTACGTCCTTTTACTTTTAATTCAGTAATAAATTCATATTTACCTGACTGACGTCTTTTCCGTTCTTCCTTACCTGCGGTTAACAACGGATTTAAAAAAGTTAGAGTGGTAGAACCTTTTATACCCGAAAGATCAata                                                                                                                                                               <  1:363873/225‑1 (MQ=255)                                                                                                              aCGTGTAACGGCATGTTGAATTAAGTTAGAAAAATCATATACACCATTAGAATTAGGAACACCTTTTACCTTCCAATCGGTACGTCCTTTTACTTTTAATTCAGTAATAAATTCATATTTACCTGACTGACGTCTTTTCCGTTCTTCCTTACCTGCGGTTAACAACGGATTTAAAAAAGTTAGAGTGGTAGAACCTTTTATACCCGAAAGATCAATATTACCTTTGATTGGATCTAAAATCTGTTTAGTGa                                                                                                                             <  1:438036/251‑1 (MQ=255)                                                                                                                gtgtAACGGCATGTTGAATTAAGTTAGAAAAATCATATACACCATTAGAATTAGGAACACCTTTTACCTTCCAATCGGTACGTCCTTTTACTTTTAATTCAGTAATAAATTCATATTTACCTGACTGACGTCTTTTCCGTTCTTCCTTACCTGCGGTTAACAACGGATTTAAAAAAGTTAGAGTGGTAGAACCTTTTATACCCGAAAGATCAATATTACCtt                                                                                                                                                        >  1:493897/1‑222 (MQ=255)                                                                                                                gtgtAACGGCATGTTGAATTAAGTTAGAAAAATCATATACACCATTAGAATTAGGAACACCTTTTACCTTCCAATCGGTACGTCCTTTTACTTTTAATTCAGTAATAAATTCATATTTACCTGACTGACGTCTTTTCCGTTCTTCCTTACCTGCGGTTAACAACGGATTTAAAAAAGTTAGAGTGGTAGAACCTTTTATACCCGAAAGATCAATATTACCTTTGATTGGATCTAAAATCTGTTTAGTGAAt                                                                                                                           >  1:145522/1‑251 (MQ=255)                                                                                                                      cGGCATGTTGAATTAAGTTAGAAAAATCATATACACCATTAGAATTAGGAACACCTTTTACCTTCCAATCGGTACGTCCTTTTACTTTTAATTCAGTAATAAATTCATATTTACCTGACTGACGTCTTTTCCGTTCTTCCTTACCTGCGGTTAACAACGGATTTAAAAAAGTTAGAGTGGTAGAACCTTTTATAc                                                                                                                                                                             >  1:187836/1‑195 (MQ=255)                                                                                                                        gCATGTTGAATTAAGTTAGAAAAATCATATACACCATTAGAATTAGGAACACCTTTTACCTTCCAATCGGTACGTCCTTTTACTTTTAATTCAGTAATAAATTCATATTTACCTGACTGACGTCTTTTCCGTTCTTCCTTACCTGCGGTTAACAACGGATTTAAAAAAGTTAGAGTGGTAGAACCTTTTATACCCGAAAGATCAATATTACCTTTGATTGGATCTAAAATCTGTTTAGTGAATTTATCGc                                                                                                                    <  1:552885/250‑1 (MQ=255)                                                                                                                             ttGAATTAAGTTAGAAAAATCATATACACCATTAGAATTAGGAACACCTTTTACCTTCCAATCGGTACGTCCTTTTACTTTTAATTCAGTAATAAATTCATATTTACCTGACTGACGTCTTTTCCGTTCTTCCTTACCTGCGGTTAACAACGGATTTAAAAAAGTTAGAGTGGTAGAACCTTTTATACCCGAAAGATCAATATTACCTTTGATTGGATCTAAAATCTGTTTAGTGAATTTATCGCTATGc                                                                                                               <  1:674301/250‑1 (MQ=255)                                                                                                                                 aTTAAGTTAGAAAAATCATATACACCATTAGAATTAGGAACACCTTTTACCTTCCAATCGGTACGTCCTTTTACTTTTAATTCAGTAATAAATTCATATTTACCTGACTGACGTCTTTTCCGTTCTTCCTTACCTGCGGTTAACAACGGATTTAAAAAAGTTAGAGTGGTAGAACCTTTTATACCCGAAAGATCAATATTACCTTTGATTGGATCTAAAATCTGTTTAGTGAATTTATCGCTATGCTTTGc                                                                                                          <  1:461344/251‑1 (MQ=255)                                                                                                                                    aaGTTAGAAAAATCATATACACCATTAGAATTAGGAACACCTTTTACCTTCCAATCGGTACGTCCTTTTACTTTTAATTCAGTAATAAATTCATATTTACCTGACTGACGTCTTTTCCGTTCTTCCTTACCTGCGGTTAACAACGGATTTAAAAAAGTTAGAGTGGTAGAACCTTTTATACCCGAAAGATCAATATTACCTTTGATTGGATCTAAAATCTGTTTAGTGAATTTATCGCTATGCTTTGCAAg                                                                                                       >  1:402292/1‑251 (MQ=255)                                                                                                                                                         ccATTAGAATTAGGAACACCTTTTACCTTCCAATCGGTACGTCCTTTTACTTTTAATTCAGTAATAAATTCATATTTACCTGACTGACGTCTTTTCCGTTCTTCCTTACCTGCGGTTAACAACGGATTTAAAAAAGTTAGAGTGGTAGAACCTTTTATACCCGAAAGATCAATATTAc                                                                                                                                                           <  1:573069/178‑1 (MQ=255)                                                                                                                                                         ccATTAGAATTAGGAACACCTTTTACCTTCCAATCGGTACGTCCTTTTACTTTTAATTCAGTAATAAATTCATATTTACCTGACTGACGTCTTTTCCGTTCTTCCTTACCTGCGGTTAACAACGGATTTAAAAAAGTTAGAGTGGTAGAACCTTTTATACCCGAAAGATCAATATTACCTTTGATTGGATCTAAAATCTGTTTAGTGAATTTATCGCTATGCTTTGCAAGCTCCTCACCCTTTTTCAaat                                                                                   >  1:140796/1‑250 (MQ=255)                                                                                                                                                                      gAACACCTTTTACCTTCCAATCGGTACGTCCTTTTACTTTTAATTCAGTAATAAATTCATATTTACCTGACTGACGTCTTTTCCGTTCTTCCTTACCTGCGGTTAACAACGGATTTAAAAAAGTTAGAGTGGTAGAACCTTTTATACCCGAAAGATCAATATTACCTTTGATTGGATCTAAAATCTGTTTAGTGAATTTATCGCTATGCTTTGCAAGCTCCTCACCCTTTTTCAAATAATCCACATAAg                                                                       <  1:164779/249‑1 (MQ=255)                                                                                                                                                                       aaCACCTTTTACCTTCCAATCGGTACGTCCTTTTACTTTTAATTCAGTAATAAATTCATATTTACCTGACTGACGTCTTTTCCGTTCTTCCTTACCTGCGGTTAACAACGGATTTAAAAAAGTTAGAGTGGTAGAACCTTTTATACCCGAAAGATCAATATTACCTTTGATTGGATCTAAAATCTGTTTAGTGAATTTATCGCTATGCTTTGCAAGCTCCTCACCCTTTTTCAAATAATCCACATAAGCt                                                                     <  1:536039/250‑1 (MQ=255)                                                                                                                                                                           ccTTTTACCTTCCAATCGGTACGTCCTTTTACTTTTAATTCAGTAATAAATTCATATTTACCTGACTGACGTCTTTTCCGTTCTTCCTTACCTGCGGTTAACAACGGATTTAAAAAAGTTAGAGTGGTAGAACCTTTTATACCCGAAAGATCAATATTACCTTTGAtt                                                                                                                                                   >  1:415814/1‑168 (MQ=255)                                                                                                                                                                           ccTTTTACCTTCCAATCGGTACGTCCTTTTACTTTTAATTCAGTAATAAATTCATATTTACCTGACTGACGTCTTTTCCGTTCTTCCTTACCTGCGGTTAACAACGGATTTAAAAAAGTTAGAGTGGTAGAACCTTTTATACCCGAAAGATCAATATTACCTTTGATTGGATCTAAAATCTGTTTAGTGAATTTATCGCTATGCTTTGCAAGCTCCTCACCCTTTTTCAAATAATCCACATAAGCTTTAc                                                                 >  1:311755/1‑250 (MQ=255)                                                                                                                                                                             ttttACCTTCCAATCGGTACGTCCTTTTACTTTTAATTCAGTAATAAATTCATATTTACCTGACTGACGTCTTTTCCGTTCTTCCTTACCTGCGGTTAACAACGGATTTAAAAAAGTTAGAGTGGTAGAACCTTTTATACCCGAAAGATCAATATTACCTTTGATTGGATCTAAAATCTGTTTAGTGAATTTATCGCTATGCTTTGCAAGCTCCTCACCCTTTTTCAAATAATCCACATAAGCTTTACCAc                                                              <  1:316756/251‑1 (MQ=255)                                                                                                                                                                             ttttACCTTCCAATCGGTACGTCCTTTTACTTTTAATTCAGTAATAAATTCATATTTACCTGACTGACGTCTTTTCCGTTCTTCCTTACCTGCGGTTAACAACGGATTTAAAAAAGTTAGAGTGGTAGAACCTTTTATACCCGAAAGATCAATATTACCTTTGATTGGATCTAAAATCTGTTTAGTGAATTTATCGCTATGCTTTGCAAGCTCCTCACCCTTTTTCAAATAATCCACATAAGCTTTACCAc                                                              <  1:236718/251‑1 (MQ=255)                                                                                                                                                                                     tCCAATCGGTACGTCCTTTTACTTTTAATTCAGTAATAAATTCATATTTACCTGACTGACGTCTTTTCCGTTCTTCCTTACCTGCGGTTAACAACGGATTTAAAAAAGTTAGAGTGGTAGAACCTTTTATACCCGAAAGATCAATATTACCTTTGATTGGATCTAAAATCTGTTTAGTGAATTTATCGCTATGCTTTGCAAGCTCCTCACCCTTTTTCAAATAATCCACATAAGCTTTACCACTTTGAATa                                                      >  1:632513/1‑251 (MQ=255)                                                                                                                                                                                         aTCGGTACGTCCTTTTACTTTTAATTCAGTAATAAATTCATATTTACCTGACTGACGTCTTTTCCGTTCTTCCTTACCTGCGGTTAACAACGGATTTAAAAAAGTTAGAGTGGTAGAACCTTTTATACCCGAAAGATCAATATTACCTTTGATTGGATCTAAAATCTGTTTAGTGAATTTATCGCTATGCTTTGCAAGCTCCTCACCCTTTTTCAAATAATCCACATAAGCTTTACCACTTTGAATACGAt                                                  <  1:546029/251‑1 (MQ=255)                                                                                                                                                                                         aTCGGTACGTCCTTTTACTTTTAATTCAGTAATAAATTCATATTTACCTGACTGACGTCTTTTCCGTTCTTCCTTACCTGCGGTTAACAACGGATTTAAAAAAGTTAGAGTGGTAGAACCTTTTATACCCGAAAGATCAATATTACCTTTGATTGGATCTAAAATCTGTTTAGTGAATTTATCGCTATGCTTTGCAAGCTCCTCACCCTTTTTCAAATAATCCACATAAGCTTTACCACTTTGAATACGAt                                                  <  1:5880/251‑1 (MQ=255)                                                                                                                                                                                           cGGTACGTCCTTTTACTTTTAATTCAGTAATAAATTCATATTTACCTGACTGACGTCTTTTCCGTTCTTCCTTACCTGCGGTTAACAACGGATTTAAAAAAGTTAGAGTGGTAGAACCTTTTATACCCGAAAGATCAATATTACCTTTGATTGGATCTAAAATCTGTTTAGTGAATTTATCGCTATGCTTTGCAAGCTCCTCACCCTTTTTCAAATAATCCACATAAGCTTTACCACTTTGAATACGAt                                                  >  1:843968/1‑249 (MQ=255)                                                                                                                                                                                                  tCCTTTTACTTTTAATTCAGTAATAAATTCATATTTACCTGACTGACGTCTTTTCCGTTCTTCCTTACCTGCGGTTAACAACGGATTTAAAAAAGTTAGAGTGGTAGAACCTTTTATACCCGAAAGATCAATATTACCTTTGATTGGATCTAAAATCTGTTTAGTGAATTTATCGCTATGCTTTGCAAGCTCCTCACCCTTTTTCAAATAATCCACATa                                                                         <  1:276110/219‑1 (MQ=255)                                                                                                                                                                                                  tCCTTTTACTTTTAATTCAGTAATAAATTCATATTTACCTGACTGACGTCTTTTCCGTTCTTCCTTACCTGCGGTTAACAACGGATTTAAAAAAGTTAGAGTGGTAGAACCTTTTATACCCGAAAGATCAATATTACCTTTGATTGGATCTAAAATCTGTTTAGTGAATTTATCGCTATGCTTTGCAAGCTCCTCACCCTTTTTCAAATAATCCACATAAGCTTTACCACTTTGAATACGATCTCCATTa                                          >  1:32887/1‑250 (MQ=255)                                                                                                                                                                                                    cttttacttttaATTCAGTAATAAATTCATATTTACCTGACTGACGTCTTTTCCGTTCTTCCTTACCTGCGGTTAACAACGGATTTAAAAAAGTTAGAGTGGTAGAACCTTTTATACCCGAAAGATCAATATTACCTTTGATTGGATCTAAAATCTGTTTAGTGAATTTATCGCTATGCTTTGCAAGCTCCTCACCCTTTTTCAAATAATCCACATAAGCTTTACCACTTTGAATACGATCTCCATTAc                                         <  1:729088/249‑1 (MQ=255)                                                                                                                                                                                                         acttttaATTCAGTAATAAATTCATATTTACCTGACTGACGTCTTTTCCGTTCTTCCTTACCTGCGGTTAACAACGGATTTAAAAAAGTTagag                                                                                                                                                                                               >  1:423708/1‑94 (MQ=255)                                                                                                                                                                                                         acttttaATTCAGTAATAAATTCATATTTACCTGACTGACGTCTTTTCCGTTCTTCCTTACCTGCGGTTAACAACGGATTTAAAAAAGTTAGAGTGGTAGAACCTTTTATAcc                                                                                                                                                                            >  1:857146/1‑113 (MQ=255)                                                                                                                                                                                                          cttttaATTCAGTAATAAATTCATATTTACCTGACTGACGTCTTTTCCGTTCTTCCTTACCTGCGGTTAACAACGGATTTAAAAAAGTTAGAGTGGTAGAACCTTTTATACCCGAAAGATCAATATTACCTTTGATTGGATCTAAAATCTGTTTAGTGAATTTATCGCTATGCTTTGCAAGCTCCTCACCCTTTTTCAAATAATCCACATAAGCTTTACCACTTTGAATa                                                      >  1:275303/1‑230 (MQ=255)                                                                                                                                                                                                          cttttaATTCAGTAATAAATTCATATTTACCTGACTGACGTCTTTTCCGTTCTTCCTTACCTGCGGTTAACAACGGATTTAAAAAAGTTAGAGTGGTAGAACCTTTTATACCCGAAAGATCAATATTACCTTTGATTGGATCTAAAATCTGTTTAGTGAATTTATCGCTATGCTTTGCAAGCTCCTCACCCTTTTTCAAATAATCCACATAAGCTTTACCACTTTGAATACGATCTCCATTACGAGTGATa                                 >  1:336502/1‑251 (MQ=255)                                                                                                                                                                                                                   cAGTAATAAATTCATATTTACCTGACTGACGTCTTTTCCGTTCTTCCTTACCTGCGGTTAACAACGGATTTAAAAAAGTTAGAGTGGTAGAACCTTTTATACCCGAAAGATCAATATTACCTTTGATTGGATCTAAAATCTGTTTAGTGAATTTATCGCTATGCTTTGCAAGCTCCTCACCCTTTTTCAAATAATCCACATAAGCTTTACCACTTTGAATACGATCTCCATTACGAGTGATACCTGCTAAt                        >  1:674976/1‑251 (MQ=255)                                                                                                                                                                                                                     gTAATAAATTCATATTTACCTGACTGACGTCTTTTCCGTTCTTCCTTACCTGCGGTTAACAACGGATTTAAAAAAGTTAGAGTGGTAGAACCTTTTATACCCGAAAGATCAATATTACCTTTGATTGGATCTAAAATCTGTTTAGTGAATTTATCGCTATGCTTTGCAAGCTCCTCACCCTTTTTCAAATAATCCACATa                                                                         >  1:783876/1‑200 (MQ=255)                                                                                                                                                                                                                     gTAATAAATTCATATTTACCTGACTGACGTCTTTTCCGTTCTTCCTTACCTGCGGTTAACAACGGATTTAAAAAAGTTAGAGTGGTAGAACCTTTTATACCCGAAAGATCAATATTACCTTTGATTGGATCTAAAATCTGTTTAGTGAATTTATCGCTATGCTTTGCAAGCTCCTCACCCTTTTTCAAATAATCCACATa                                                                         <  1:489159/200‑1 (MQ=255)                                                                                                                                                                                                                     gTAATAAATTCATATTTACCTGACTGACGTCTTTTCCGTTCTTCCTTACCTGCGGTTAACAACGGATTTAAAAAAGTTAGAGTGGTAGAACCTTTTATACCCGAAAGATCAATATTACCTTTGATTGGATCTAAAATCTGTTTAGTGAATTTATCGCTATGCTTTGCAAGCTCCTCACCCTTTTTCAAATAATCCACATAAGCTTTACCACTTTGAATACGATCTCCATTACGAGTGATACCTGCTAAtt                       >  1:239005/1‑250 (MQ=255)                                                                                                                                                                                                                                atatTTACCTGACTGACGTCTTTTCCGTTCTTCCTTACCTGCGGTTAACAACGGATTTAAAAAAGTTAGAGTGGTAGAACCTTTTATACCCGAAAGATCAATATTACCTTTGATTGGATCTAAAATCTGTTTAGTGAATTTATCGCTATGCTTTGCAAGCTCCTCACCCTTTTTCAAATAATCCACATAAGCTTTACCACTTTGAATACGAt                                                  >  1:380259/1‑212 (MQ=255)                                                                                                                                                                                                                                     tACCTGACTGACGTCTTTTCCGTTCTTCCTTACCTGCGGTTAACAACGGATTTAAAAAAGTTAGAGTGGTAGAACCTTTTATACCCGAAAGATCAATATTACCTTTGATTGGATCTAAAATCTGTTTAGTGAATTTATCGCTATGCTTTGCAAGCTCCTCACCCTTTTTCAAATAATCCACATAAGCTTTACCACTTTGAATACGATCTCCATTACGAGTGATACCTGCTAATTCACCAATGCGCTGATc       >  1:899823/1‑250 (MQ=255)                                                                                                                                                                                                                                         tgactgacGTCTTTTCCGTTCTTCCTTACCTGCGGTTAACAACGGATTTAAAAAAGTTAGAGTGGTAGAACCTTTTATACCCGAAAGATCAATATTACCTTTGATTGGATCTAAAATCTGTTTAGTGAATTTATCGCTATGCTTTGCAAGCTCCTCACCCTTTTTCAAATAATCCACATAAGCTTTACCACTTTGAATACGATCTCCATTACGAGTGATACCTGCTAATTCACCAATGCGCTGATCCCAAc  <  1:871525/251‑1 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                 CATCATAACCGTCTCCAGCATTAACTATTGTTGAACCGGATCCGACAAAAACATAATCATCTTTAGCACCCAAATTAGCAATTAATCTTGCTTCTAGAACTTTATTATCACGTGTAACGGCATGTTGAATTAAGTTAGAAAAATCATATACACCATTAGAATTAGGAACACCTTTTACCTTCCAATCGGTACGTCCTTTTACTTTTAATTCAGTAATAAATTCATATTTACCTGACTGACGTGTTTTCCGTTCTTCCTTACCTGCGGTTAACAACGGATTTAAAAAAGTTAGAGTGGTAGAACCTTTTATACCCGAAAGATCAATATTACCTTTGATTGGATCTAAAATCTGTTTAGTGAATTTATCGCTATGCTTTGCAAGCTCCTCACCCTTTTTCAAATAATCCACATAAGCTTTACCACTTTGAATACGATCTCCATTACGAGTGATACCTGCTAATTCACCAATGCGCTGATCCCAAC  >  1/1980215‑1980697

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑