Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,979,958	G→A	S723S (AGC→AGT)	unknown ←	RTX toxins determinant A and related Ca2+‑binding proteins

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,979,958	0	G	A	100.0%	125.4 / NA	45	S723S (AGC→AGT)	unknown	RTX toxins determinant A and related Ca2+‑binding proteins
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (19/26);  total (19/26)

GTCGTCGCCTTCTCCACCGTCAAGATAGTTGTTGCCATATTCACCAAAGAGCCTATCGTTACCGGCACCACCATAGAGCTTATCATTACCTTGGCCGCCATGAATTTCGTCATTCCCATTATCGCCATACAAACGATCATCGCCATCATAACCATAAATCAAATCATCGCCATCGTGACCATGGAAAACATCATTAAATTTAGAACCATAAAATTTATCACGTAGTGTGCTGCCGATAATTTCCTCTACTGAGTTTAATTCATCATGAGCTTTAAAGCCCTTATTCGTATAATAGTAATCTAATTTATAGTTACGATATTCAAGTAGATCGGTAACCTTCCCTCGTTTAGTTTCTTGCTTTGATACGGTTTCCTGCAAGACTTGAGTACCGCTAAGATCTCTTTCAACCTTATATTGTCCGGCTTTAGTAGCATTACGACCGTCGATT  >  1/1979730‑1980177                                                                                                                                                                                                                                     |                                                                                                                                                                                                                            gtcgtcGCCTTCACCACCGTCTAGATAGTTGTTACCATATTCACCAAAGAGCCTGTCATTGCCGGCGCCACCATAAAGTTTGTCGTTGCCTTTTCCTCCATGGATCTCATCATTACCATTATCTCCATATAAGCGGTCATCACCATCATAACCATAAATCACATCATCACCATCGTGACCATGGAAAACATCATTAAATCTAGAACCATAAAAATGATCCCGTAATTTACTGCCGATAATTTCCTCTAc                                                                                                                                                                                                         <  1:420430/249‑1 (MQ=255)     tcgCCTTCACCACCGTCTAGATAGTTGTTACCATATTCACCAAAGAGCCTGTCATTGCCGGCGCCACCATAAAGTTTGTCGTTGCCTTTTCCTCCATGGATCTCATCATTACCATTATCTCCATATAAGCGGTCATCACCATCATAACCATAAATCACATCATCACCATCGTGACCATGGAAAACATCATTAAATCTAGAACCATAAAAATGATCCCGTAATTTACTGCCGATAATTTCCTCTACTGAGt                                                                                                                                                                                                    <  1:191139/250‑1 (MQ=255)       gCCTTCACCACCGTCTAGATAGTTGTTACCATATTCACCAAAGAGCCTGTCATTGCCGGCGCCACCATAAAGTTTGTCGTTGCCTTTTCCTCCATGGATCTCATCATTACCATTATCTCCATATAAGCGGTCATCACCATCATAACCATAAATCACATCATCACCATCGTGACCATGGAAAACATCATTAAATCTAGAACCATAAAAATGATCCCGTAATTTACTGCCGATAATTTCCTCTACTGAGTTTa                                                                                                                                                                                                 >  1:570569/1‑251 (MQ=255)        ccTTCACCACCGTCTAGATAGTTGTTACCATATTCACCAAAGAGCCTGTCATTGCCGGCGCCACCATAAAGTTCGTCGTTGCCTTTTCCTCCATGGATCTCATCATTACCATTATCTCCATATAAGCGGTCATCACCATCATAACCATAAATCACATCATCACCATCGTGACCATGGAAAACATCATTAAATCTAGAACCATAAAAATGATCCCGTAATTTACTGCCGATAATTTCCTCTAc                                                                                                                                                                                                         <  1:751858/242‑1 (MQ=255)           tcaccACCGTCTAGATAGTTGTTACCATATTCACCAAAGAGCCTGTCATTGCCGGCGCCACCATAAAGTTTGTCGTTGCCTTTTCCTCCATGGATCTCATCATTACCATTATCTCCATATAAGCGGTCATCACCATCATAACCATAAATCACATCATCACCATCGTGACCATGGAAAACATCATTAAATCTAGAACCATAAAAATGATCCCGTAATTTACTGCCGATAATTTCCTCTACTGAGTTTAATtc                                                                                                                                                                                             <  1:588716/248‑1 (MQ=255)           tcaccACCGTCTAGATAGTTGTTACCATATTCACCAAAGAGCCTGTCATTGCCGGCGCCACCATAAAGTTTGTCGTTGCCTTTTCCTCCATGGATCTCATCATTACCATTATCTCCATATAAGCGGTCATCACCATCATAACCATAAATCACATCATCACCATCGTGACCATGGAAAACATCATTAAATCTAGAACCATAAAAATGATCCCGTAATTTACTGCCGATAATTTCCTCTACTGAGTTTAATtc                                                                                                                                                                                             <  1:671199/248‑1 (MQ=255)                 ccGTCTAGATAGTTGTTACCATATTCACCAAAGAGCCTGTCATTGCCGGCGCCACCATAAAGTTTGTCGTTGCCTTTTCCTCCATGGATCTCATCATTACCATTATCTCCATATAAGCGGTCATCACCATCATAACCATAAATCACATCATCACCATCGTGACCATGGAAAACATCATTAAATCTAGAACCATAAAAATGATCCCGTAATTTACTGCCGATAATTTCCTCTACTGAGTTTAATTCATCATg                                                                                                                                                                                       >  1:332492/1‑251 (MQ=255)                            gttgttACCATATTCACCAAAGAGCCTGTCATTGCCGGCGCCACCATAAAGTTTGTCGTTGCCTTTTCCTCCATGGATCTCATCATTACCATTATCTCCATATAAGCGGTCATCACCATCATAACCATAAATCACATCATCACCATCGTGACCATGGAAAACATCATTAAATCTAGAACCATAAAAATGATCCCGTAATTTACTGCCGATAATTTCCTCTACTg                                                                                                                                                                                                       <  1:521832/224‑1 (MQ=255)                              tgttaCCATATTCACCAAAGAGCCTGTCATTGCCGGCGCCACCATAAAGTTTGTCGTTGCCTTTTCCTCCATGGATCTCATCATTACCATTATCTCCATATAAGCGGTCATCACCATCATAACCATAAATCACATCATCACCATCGTGACCATGGAAAACATCATTAAATCTAGAACCATAAAAATGATCCCGTAATTTACTGCCGATAATTTCCTCTACTGAGTTTAATTCATCATGAGCTTTAAAGccc                                                                                                                                                                          <  1:392856/251‑1 (MQ=255)                                   ccATATTCACCAAAGAGCCTGTCATTGCCGGCGCCACCATAAAGTTTGTCGTTGCCTTTTCCTCCATGGATCTCATCATTATCATTATCTCCATATAAGCGGTCATCACCATCATAACCATAAATCACATCATCACCATCGTGACCATGGAAAACATCATTAAATCTAGAACCATAAAAATGATCCCGTAATTTACTGCCGATAATTTCCTCTACTGAGTTTAATTCATCATGAGCTTTAAAGCCCTTAtt                                                                                                                                                                     <  1:429599/251‑1 (MQ=255)                                   ccATATTCACCAAAGAGCCTGTCATTGCCGGCGCCACCATAAAGTTTGTCGTTGCCTTTTCCTCCATGGATCTCATCATTACCATTATCTCCATATAAGCGGTCATCACCATCATAACCATAAATCACATCATCACCATCGTGACCATGGAAAACATCATTAAATCTAGAACCATAAAAATGATCCCGTAATTTACTGCCGATAATTTCCTCTACTGAGTTTAATTCATCATGAGCTTTAAAGCCCTTAtt                                                                                                                                                                     <  1:159658/251‑1 (MQ=255)                                   ccATATTCACCAAAGAGCCTGTCATTGCCGGCGCCACCATAAAGTTTGTCGTTGCCTTTTCCTCCATGGATCTCATCATTACCATTATCTCCATATAAGCGGTCATCACCATCATAACCATAAATCACATCATCACCATCGTGACCATGGAAAACATCATTAAATCTAGAACCATAAAAATGATCCCGTAATTTACTGCCGATAATTTCCTCTACTGAGTTTAATTCATCATGAGCTTTAAAGCCCTTAtt                                                                                                                                                                     <  1:185394/251‑1 (MQ=255)                                       atTCACCAAAGAGCCTGTCATTGCCGGCGCCACCATAAAGTTTGTCGTTGCCTTTTCCTCCATGGATCTCATCATTACCATTATCTCCATATAAGCGGTCATCACCATCATAACCATAAATCACATCATCACCATCGTGACCATGGAAAACATCATTAAATCTAGAACCATAAAAATGATCCCGTAATTTACTGCCGATAATTTCCTCTACTGAGTTTAATTCATCATGAGc                                                                                                                                                                                    <  1:645074/232‑1 (MQ=255)                                          cACCAAAGAGCCTGTCATTGCCGGCGCCACCATAAAGTTTGTCGTTGCCTTTTCCTCCATGGATCTCATCATTACCATTATCTCCATATAAGCGGTCATCACCATCATAACCATAAATCACATCATCACCATCGTGACCATGGAAAACATCATTAAATCTAGAACCATAAAAATGATCCCGTAATTTACTGCCGATAATTTCCTCTACTGAGTTTAATTCATCATGAGCTTTAAAGCCCTTAtt                                                                                                                                                                     <  1:470858/244‑1 (MQ=255)                                            ccAAAGAGCCTGTCATTGCCGGCGCCACCATAAAGTTTGTCGTTGCCTTTTCCTCCATGGATCTCATCATTACCATTATCTCCATATAAGCGGTCATCACCATCATAACCATAAATCACATCATCACCATCGTGACCATGGAAAACATCATTAAATCTAGAACCATAAAAATGATCCCGTAATTTACTGCCGATAATTTCCTCTACTGAGTTTAATTCATCATGAGCTTTAAAGCCCTTATTCGTataata                                                                                                                                                            >  1:141191/1‑251 (MQ=255)                                             cAAAGAGCCTGTCATTGCCGGCGCCACCATAAAGTTTGTCGTTGCCTTTTCCTCCATGGATCTCATCATTACCATTATCTCCATATAAGCGGTCATCACCATCATAACCATAAATCACATCATCACCATCGTGACCATGGAAAACATCATTAAATCTAGAACCATAAAAATGATCCCGTAATTTACTGCCGATAATTTCCTCTACTGAGTTTAATTCATCATGAGCTTTAAAGCCCTTATTCGTATAATAg                                                                                                                                                           <  1:557348/251‑1 (MQ=255)                                             cAAAGAGCCTGTCATTGCCGGCGCCACCATAAAGTTTGTCGTTGCCTTTTCCTCCATGGATCTCATCATTACCATTATCTCCATATAAGCGGTCATCACCATCATAACCATAAATCACATCATCACCATCGTGACCATGGAAAACATCATTAAATCTAGAACCATAAAAATGATCCCGTAATTTACTGCCGATAATTTCCTCTACTGAGTTTAATTCATCATGAGCTTTAAAGCCCTTATTCGTATAATAg                                                                                                                                                           <  1:152925/251‑1 (MQ=255)                                                                                               cctccatggatctcatCATTACCATTATCTCCATATAAGCGGTCATCACCATCATAACCATAAATCACATCATCACCATCGTGACCATGGAAAACATCATTAAATCTAGAACCATAAAAATGATCCCGTAATTTACTGCCGATAATTTCCTCTACTGAGTTTAATTCATCATGAGCTTTAAAGCCCTTATTCGTATAATAGTAATCTAATTTATAGTTACGATATTCAAGTAGATCGGTAACCTTCCCTCg                                                                                                         >  1:16182/16‑251 (MQ=255)                                                                                                       gatctcatCATTACCATTATCTCCATATAAGCGGTCATCACCATCATAACCATAAATCACATCATCACCATCGTGACCATGGAAAACATCATTAAATCTAGAACCATAAAAATGATCCCGTAATTTACTGCCGATAATTTCCTCTACTGAGTTTAATTCATCATGAGCTTTAAAGCCCTTATTCGTATAATAg                                                                                                                                                           <  1:807841/186‑1 (MQ=255)                                                                                                       gatctcatCATTACCATTATCTCCATATAAGCGGTCATCACCATCATAACCATAAATCACATCATCACCATCGTGACCATGGAAAACATCATTAAATCTAGAACCATAAAAATGATCCCGTAATTTACTGCCGATAATTTCCTCTACTGAGTTTAATTCATCATGAGCTTTAAAGCCCTTATTCGTATAATAGTAATCTAATTTATAg                                                                                                                                            >  1:516618/8‑208 (MQ=255)                                                                                                        atctcatCATTACCATTATCTCCATATAAGCGGTCATCACCATCATAACCATAAATCACATCATCACCATCGTGACCATGGAAAACATCATTAAATCTAGAACCATAAAAATGATCCCGTAATTTACTGCCGATAATTTCCTCTACTGAGTTTAAt                                                                                                                                                                                               <  1:773700/150‑1 (MQ=255)                                                                                                            catCATTACCATTATCTCCATATAAGCGGTCATCACCATCATAACCATAAATCACATCATCACCATCGTGACCATGGAAAACATCATTAAATCTAGAACCATAAAAATGATCCCGTAATTTACTGCCGATAATTTCCTCTACTGAGTTTAATTCATCATGAGCTTTAAAGCCCTTATTc                                                                                                                                                                    <  1:282369/177‑1 (MQ=255)                                                                                                            catCATTACCATTATCTCCATATAAGCGGTCATCACCATCATAACCATAAATCACATCATCACCATCGTGACCATGGAAAACATCATTAAATCTAGAACCATAAAAATGATCCCGTAATTTACTGCCGATAATTTCCTCTACTGAGTTTAATTCATCATGAGCTTTAAAGCCCTTATTCGTATAATAg                                                                                                                                                           >  1:161063/3‑188 (MQ=255)                                                                                                                 ttaccATTATCTCCATATAAGCGGTCATCACCATCATAACCATAAATCACATCATCACCATCGTGACCATGGAAAACATCATTAAATCTAGAACCATAAAAATGATCCCGTAATTTACTGCCGATAATTTCCTCTACTGAGTTTAATTCATCATGAGCTTTAAAGCCCTTATTCGTATAATAg                                                                                                                                                           <  1:828412/180‑1 (MQ=255)                                                                                                                 ttaccATTATCTCCATATAAGCGGTCATCACCATCATAACCATAAATCACATCATCACCATCGTGACCATGGAAAACATCATTAAATCTAGAACCATAAAAATGATCCCGTAATTTACTGCCGATAATTTCCTCTACTGAGTTTAATTCATCATGAGCTTTAAAGCCCTTATTCGTATAATAGTAATCTAATTTATAGTTACGATATTCAAGTAGATCGGTAACCTTCCCTCGTTTAGTTTCTTGCTTTGa                                                                                       >  1:694552/4‑251 (MQ=255)                                                                                                                             ccatataagcggtcatcaCCATCATAACCATAAATCACATCATCACCATCGTGACCATGGAAAACATCATTAAATCTAGAACCATAAAAATGATCCCGTAATTTACTGCCGATAATTTCCTCTACTGAGTTTAATTCATCATGAGCTTTAAAGCCCTTAtt                                                                                                                                                                     >  1:878970/13‑161 (MQ=255)                                                                                                                             ccatataagcggtcatcaCCATCATAACCATAAATCACATCATCACCATCGTGACCATGGAAAACATCATTAAATCTAGAACCATAAAAATGATCCCGTAATTTACTGCCGATAATTTCCTCTACTGAGTTTAATTCATCATGAGCTTTAAAGCCCTTAtt                                                                                                                                                                     >  1:108659/13‑161 (MQ=255)                                                                                                                             ccatataagcggtcatcaCCATCATAACCATAAATCACATCATCACCATCGTGACCATGGAAAACATCATTAAATCTAGAACCATAAAAATGATCCCGTAATTTACTGCCGATAATTTCCTCTACTGAGTTTAATTCATCATGAGCTTTAAAGCCCTTATTCGTATAATAg                                                                                                                                                           >  1:723137/13‑171 (MQ=255)                                                                                                                             ccatataagcggtcatcaCCATCATAACCATAAATCACATCATCACCATCGTGACCATGGAAAACATCATTAAATCTAGAACCATAAAAATGATCCCGTAATTTACTGCCGATAATTTCCTCTACTGAGTTTAATTCATCATGAGCTTTAAAGCCCTTATTCGTATAATAGTAATCt                                                                                                                                                     >  1:217626/13‑177 (MQ=255)                                                                                                                                           atcaccATCATAACCATAAATCACATCATCACCATCGTGACCATGGAAAACATCATTAAATCTAGAACCATAAAAATGATCCCGTAATTTACTGCCGATAATTTCCTCTACTGAGTTTAATTCATCATGAGCTTTAAAGCCCTTATTCGTATAATAGTAATCTAATTTATAGTTACGATATTCAAGTAGATCGGTAACCTTCCCTCGTTTAGTTTCTTGCTTTGATACGGTTTCCTGCAAGACTTGAGTAc                                                             <  1:794137/247‑1 (MQ=255)                                                                                                                                                catcatAACCATAAATCACATCATCACCATCGTGACCATGGAAAACATCATTAAATCTAGAACCATAAAAATGATCCCGTAATTTACTGCCGATAATTTCCTCTACTGAGTTTAATtc                                                                                                                                                                                             >  1:232746/1‑118 (MQ=255)                                                                                                                                                       aCCATAAATCACATCATCACCATCGTGACCATGGAAAACATCATTAAATCTAGAACCATAAAAATGATCCCGTAATTTACTGCCGATAATTTCCTCTACTGAGTTTAATtc                                                                                                                                                                                             >  1:845953/1‑111 (MQ=255)                                                                                                                                                        ccATAAATCACATCATCACCATCGTGACCATGGAAAACATCATTAAATCTAGAACCATAAAAATGATCCCGTAATTTACTGCCGATAATTTCCTCTACTGAGTTTAATTCATCATGAGCTTTAAAGCCCTTATTCGTATAATAGTAATCTAATTTATAGTTACGATATTCAAGTAGATCGGTAACCTTCCCTCGTTTAGTTTCTTGCTTTGATACGGTTTCCTGCAAGACTTGAGTACCGCTAAGa                                                     >  1:507207/1‑246 (MQ=255)                                                                                                                                                                     catcACCATCGTGACCATGGAAAACATCATTAAATCTAGAACCATAAAAATGATCCCGTAATTTACTGCCGATAATTTCCTCTACTGAGTTTAATTCATCATGAGCTTTAAAGCCCTTATTCGTATAATAGTAATCTAATTTATAGTTACGATATTCAAGTAGATCGGTAACCTTCCCTCGTTTAGTTTCTTGCTTTGATACGGTTTCCTGCAAGACTTGAGTACCGCTAAGATCTCTTTCAACCTTatat                                   <  1:872250/251‑1 (MQ=255)                                                                                                                                                                     catcACCATCGTGACCATGGAAAACATCATTAAATCTAGAACCATAAAAATGATCCCGTAATTTACTGCCGATAATTTCCTCTACTGAGTTTAATTCATCATGAGCTTTAAAGCCCTTATTCGTATAATAGTAATCTAATTTATAGTTACGATATTCAAGTAGATCGGTAACCTTCCCTCGTTTAGTTTCTTGCTTTGATACGGTTTCCTGCAAGACTTGAGTACCGCTAAGATCTCTTTCAACCTTatat                                   <  1:606260/251‑1 (MQ=255)                                                                                                                                                                     catcACCATCGTGACCATGGAAAACATCATTAAATCTAGAACCATAAAAATGATCCCGTAATTTACTGCCGATAATTTCCTCTACTGAGTTTAATTCATCATGAGCTTTAAAGCCCTTATTCGTATAATAGTAATCTAATTTATAGTTACGATATTCAAGTAGATCGGTAACCTTCCCTCGTTTAGTTTCTTGCTTTGATACGGTTTCCTGCAAGACTTGAGTACCGCTAAGATCTCTTTCAACCTTatat                                   <  1:244104/251‑1 (MQ=255)                                                                                                                                                                     catcACCATCGTGACCATGGAAAACATCATTAAATCTAGAACCATAAAAATGATCCCGTAATTTACTGCCGATAATTTCCTCTACTGAGTTTAATTCATCATGAGCTTTAAAGCCCTTATTCGTATAATAGTAATCTAATTTATAGTTACGATATTCAAGTAGATCGGTAACCTTCCCTCGTTTAGTTTCTTGCTTTGATACGGTTTCCTGCAAGACTTGAGTACCGCTAAGATCTCTTTCAACCTTatat                                   <  1:729921/251‑1 (MQ=255)                                                                                                                                                                              cGTGACCATGGAAAACATCATTAAATCTAGAACCATAAAAATGATCCCGTAATTTACTGCCGATAATTTCCTCTACTGAGTTTAATTCATCATGAGCTTTAAAGCCCTTATTCGTATAATAGTAATCTAATTTATAGTTACGATATTCAAGTAGATCGGTAACCTTCCCTCGTTTAGTTTCTTGCTTTGATACGGTTTCCTGCAAGACTTGAGTACCGCTAAGATCTCTTTCAACCTTATATTGTCCGGCt                          <  1:301135/251‑1 (MQ=255)                                                                                                                                                                                  aCCATGGAAAACATCATTAAATCTAGAACCATAAAAATGATCCCGTAATTTACTGCCGATAATTTCCTCTACTGAGTTTAATTCATCATGAGCTTTAAAGCCCTTATTCGTATAATAGTAATCTAAtt                                                                                                                                                 <  1:23876/128‑1 (MQ=255)                                                                                                                                                                                                     aatctAGAACCATAAAAATGATCCCGTAATTTACTGCCGATAATTTCCTCTACTGAGTTTAATTCATCATGAGCTTTAAAGCCCTTATTCGTATAATAGTAATCTAATTTATAGTTACGATATTCAAGTAGATCGGTAACCTTCCCTCGTTTAGTTTCTTGCTTTGATACGGTTTCCTGCAAGACTTGAGTACCGCTAAGATCTCTTTCAACCTTATATTGTCCGGCTTTAGTAGCATTACGACCGTCGAt   <  1:147621/247‑1 (MQ=255)                                                                                                                                                                                                      atctAGAACCATAAAAATGATCCCGTAATTTACTGCCGATAATTTCCTCTACTGAGTTTAAt                                                                                                                                                                                               >  1:120811/4‑62 (MQ=255)                                                                                                                                                                                                      atctAGAACCATAAAAATGATCCCGTAATTTACTGCCGATAATTTCCTCTACTGAGTTTAATTCATCATGAGCTTTAAAg                                                                                                                                                                             >  1:23059/4‑80 (MQ=255)                                                                                                                                                                                                      atctAGAACCATAAAAATGATCCCGTAATTTACTGCCGATAATTTCCTCTACTGAGTTTAATTCATCATGAGCTTTAAAGCCCTTATTCGTATAATAGTAATCTAATTTATAGTTACGATATTCAAGTAGATCGGTAACCTTCCCTCGTTTAGTTTCTTGCTTTGATACGGTTTCCTGCAAGACTTGAGTAc                                                             >  1:673442/4‑192 (MQ=255)                                                                                                                                                                                                      atctAGAACCATAAAAATGATCCCGTAATTTACTGCCGATAATTTCCTCTACTGAGTTTAATTCATCATGAGCTTTAAAGCCCTTATTCGTATAATAGTAATCTAATTTATAGTTACGATATTCAAGTAGATCGGTAACCTTCCCTCGTTTAGTTTCTTGCTTTGATACGGTTTCCTGCAAGACTTGAGTACCGCTAAGATCTCTTTCAACCTTATATTGTCCGGCTTTAGTAGCATTACGACCGTCGAtt  >  1:605816/4‑251 (MQ=255)                                                                                                                                                                                                      atctAGAACCATAAAAATGATCCCGTAATTTACTGCCGATAATTTCCTCTACTGAGTTTAATTCATCATGAGCTTTAAAGCCCTTATTCGTATAATAGTAATCTAATTTATAGTTACGATATTCAAGTAGATCGGTAACCTTCCCTCGTTTAGTTTCTTGCTTTGATACGGTTTCCTGCAAGACTTGAGTACCGCTAAGATCTCTTTCAACCTTATATTGTCCGGCTTTAGTAGCATTACGACCGTCGAtt  >  1:464941/4‑251 (MQ=255)                                                                                                                                                                                                                                     |                                                                                                                                                                                                                            GTCGTCGCCTTCTCCACCGTCAAGATAGTTGTTGCCATATTCACCAAAGAGCCTATCGTTACCGGCACCACCATAGAGCTTATCATTACCTTGGCCGCCATGAATTTCGTCATTCCCATTATCGCCATACAAACGATCATCGCCATCATAACCATAAATCAAATCATCGCCATCGTGACCATGGAAAACATCATTAAATTTAGAACCATAAAATTTATCACGTAGTGTGCTGCCGATAATTTCCTCTACTGAGTTTAATTCATCATGAGCTTTAAAGCCCTTATTCGTATAATAGTAATCTAATTTATAGTTACGATATTCAAGTAGATCGGTAACCTTCCCTCGTTTAGTTTCTTGCTTTGATACGGTTTCCTGCAAGACTTGAGTACCGCTAAGATCTCTTTCAACCTTATATTGTCCGGCTTTAGTAGCATTACGACCGTCGATT  >  1/1979730‑1980177

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑