Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	309,537	C→A	G105V (GGG→GTG)	unknown ←	Recombinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	309,537	0	C	A	100.0%	224.3 / NA	78	G105V (GGG→GTG)	unknown	Recombinase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (49/29);  total (49/29)

CATCAGCACTTAATAATAGGCGTCTTAACCCAAATCCAGCTGGTCCACCTTGACGAAATCCGCGCTCAATCAAATTCTTCTGCCCATTAAAAACTTTTTCACCTAGCTCACGACAGTATTCTCCGGCAGACCAACGTTTTACCGCTTTAAAAATTTGAGAACCAATGTCGTTTCCCTTAAAAGGTTCTGCACAGTAATGCACTTTAATCCCCATTTTTGAACAAATTTGTTCATAATGTGCAGATTCATCAATATTTAGAAAGCGCCCCCATCGGCTAATGTCATAAACAAGAATATGTGAAAAATCAGCATTACCGGATTGTACAAAATTAATGAGTGATAAAAACTGAGGACGTTTTTCTGCGCTTAATCCACTCCTTCCCTCATCTAGGTATTCCCTAGTGAGTATAAGCCCTTGTTCCTTAGCATATTTTTGAATGAAGCGACGTTGTATAT  >  1/309328‑309783                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                       cATCAGCACTTAATAATAGGCGTCTTAACCCAAATCCAGCTGGTCCACCTTGACGAAATCCGCGCTCAATCAAATTCTTCTGCCCATTAAAAACTTTTTCACCTAGCTCACGACAGTATTCTCCGGCAGACCAACGTTTTACCGCTTTAAAAATTTGAGAACCAATGTCGTTTCCCTTAAAAGGTTCTGCACAGTAATGCACTTTAATCACCATTTTTGAACAAATTTGTTCATAATGTGCAGATtcatc                                                                                                                                                                                                                <  1:232738/250‑1 (MQ=255)       cACTTAATAATAGGCGTCTTAACCCAAATCCAGCTGGTCCACCTTGACGAAATCCGCGCTCAATCAAATTCTTCTGCCCATTAAAAACTTTTTCACCTAGCTCACGACAGTATTCTCCGGCAGACCAACGTTTTACCGCTTTAAAAATTTGAGAACCAATGTCGTTTCCCTTAAAAGGTTCTGCACAGTAATGCACTTTAATCACCATTTTTGAACAAATTTGTTCATAATGTGCAGATTCATCAATAttt                                                                                                                                                                                                         <  1:22620/251‑1 (MQ=255)         cTTAATAATAGGCGTCTTAACCCAAATCCAGCTGGTCCACCTTGACGAAATCCGCGCTCAATCAAATTCTTCTGCCCATTAAAAACTTTTTCACCTAGCTCACGACAGTATTCTCCGGCAGACCAACGTTTTACCGCTTTAAAAATTTGAGAACCAATGTCGTTTCCCTTAAAAGGTTCTGCACAGTAATGCACTTTAATCACCATTTTTGAACAAATTTGTTCATAATGTGCAGATTCATCAATATTTAg                                                                                                                                                                                                       >  1:740994/1‑251 (MQ=255)         cTTAATAATAGGCGTCTTAACCCAAATCCAGCTGGTCCACCTTGACGAAATCCGCGCTCAATCAAATTCTTCTGCCCATTAAAAACTTTTTCACCTAGCTCACGACAGTATTCTCCGGCAGACCAACGTTTTACCGCTTTAAAAATTTGAGAACCAATGTCGTTTCCCTTAAAAGGTTCTGCACAGTAATGCACTTTAATCACCATTTTTGAACAAATTTGTTCATAATGTGCAGATTCATCAATATTTAg                                                                                                                                                                                                       >  1:454831/1‑251 (MQ=255)         cTTAATAATAGGCGTCTTAACCCAAATCCAGCTGGTCCACCTTGACGAAATCCGCGCTCAATCAAATTCTTCTGCCCATTAAAAACTTTTTCACCTAGCTCACGACAGTATTCTCCGGCAGACCAACGTTTTACCGCTTTAAAAATTTGAGAACCAATGTCGTTTCCCTTAAAAGGTTCTGCACAGTAATGCACTTTAATCACCATTTTTGAACAAATTTGTTCATAATGTGCAGATTCATCAATATTTAg                                                                                                                                                                                                       >  1:687745/1‑251 (MQ=255)           taataaTAGGCGTCTTAACCCAAATCCAGCTGGTCCACCTTGACGAAATCCGCGCTCAATCAAATTCTTCTGCCCATTAAAAACTTTTTCACCTAGCTCACGACAGTATTCTCCGGCAGACCAACGTTTTACCGCTTTAAAAATTTGAGAACCAATGTCGTTTCCCTTAAAAGGTTCTGCACAGTAATGCACTTTAATCACCATTTTTGAACAAATTTGTTCATAATGTGCAGATTCATCAATATTTAGaa                                                                                                                                                                                                     >  1:186226/1‑251 (MQ=255)           taataaTAGGCGTCTTAACCCAAATCCAGCTGGTCCACCTTGACGAAATCCGCGCTCAATCAAATTCTTCTGCCCATTAAAAACTTTTTCACCTAGCTCACGACAGTATTCTCCGGCAGACCAACGTTTTACCGCTTTAAAAATTTGAGAACCAATGTCGTTTCCCTTAAAAGGTTCTGCACAGTAATGCACTTTAATCACCATTTTTGAACAAATTTGTTCATAATGTGCAGATTCATCAATATTTAGaa                                                                                                                                                                                                     >  1:512202/1‑251 (MQ=255)             ataataGGCGTCTTAACCCAAATCCAGCTGGTCCACCTTGACGAAATCCGCGCTCAATCAAATTCTTCTGCCCATTAAAAACTTTTTCACCTAGCTCACGACAGTATTCTCCGGCAGACCAACGTTTTACCGCTTTAAAAATTTGAGAACCAATGTCGTTTCCCTTAAAAGGTTCTGCACAGTAATGCACTTTAATCACCATTTTTGAACAAATTTGTTCATAATGTGCAGATTCATCAATATTTAGAAAg                                                                                                                                                                                                   >  1:376535/1‑251 (MQ=255)                       tCTTAACCCAAATCCAGCTGGTCCACCTTGACGAAATCCGCGCTCAATCAAATTCTTCTGCCCATTAAAAACTTTTTCACCTAGCTCACGACAGTATTCTCCGGCAGACCAACGTTTTACCGCTTTAAAAATTTGAGAACCAATGTCGTTTCCCTTAAAAGGTTCTGCACAGTAATGCACTTTAATCACCATTTTTGAACAAATTTGTTCATAATGTGCAGATTCATCAATATTTAGAAAGCGCCCCCAt                                                                                                                                                                                          >  1:799027/1‑250 (MQ=255)                                                                     atcaaATTCTTCTGCCCATTAAAAACTTTTTCACCTAGCTCACGACAGTATTCTCCGGCAGACCAACGTTTTACCGCTTTAAAAATTTGAGAACCAATGTCGTTTCCCTTAAAAGGTTCTGCACAGTAATGCACTTTAATCACCATTTTTGAACAAATTTGTTCATAATGTGCAGATTCATCAATATTTAGAAAGCGCCCCCATCGGCTAATGTCATAAACAAGAATATGTGAAAAATCAGCATTACCGGa                                                                                                                                           >  1:8298/1‑251 (MQ=255)                                                                      tcaaATTCTTCTGCCCATTAAAAACTTTTTCACCTAGCTCACGACAGTATTCTCCGGCAGACCAACGTTTTACCGCTTTAAAAATTTGAGAACCAATGTCGTTTCCCTTAAAAGGTTCTGCACAGTAATGCACTTTAATCACCATTTTTGAACAAATTTGTTCATAATGTGCAGATTCATCAATATTTAGAAAGCGCCCCCATCGGCTAATGTCATAAACAAGAATATGTGAAAAATCAGCATTACCGGAt                                                                                                                                          <  1:52461/251‑1 (MQ=255)                                                                             cttctGCCCATTAAAAACTTTTTCACCTAGCTCACGACAGTATTCTCCGGCAGACCAACGTTTTACCGCTTTAAAAATTTGAGAACCAATGTCGTTTCCCTTAAAAGGTTCTGCACAGTAATGCACTTTAATCACCATTTTTGAACAAATTTGTTCATAATGTGCAGATTCATCAATATTTAGAAAGCGCCCCCATCGGCTAATGTCATAAACAAGAATATGTGAAAAATCAGCATTACCGGATTGTACaa                                                                                                                                   >  1:873352/1‑251 (MQ=255)                                                                                       ttAAAAACTTTTTCACCTAGCTCACGACAGTATTCTCCGGCAGACCAACGTTTTACCGCTTTAAAAATTTGAGAACCAATGTCGTTTCCCTTAAAAGGTTCTGCACAGTAATGCACTTTAATCACCATTTTTGAACAAATTTGTTCATAATGTGCAGATTCATCAATATTTAGAAAGCGCCCCCATCGGCTAATGTCATAAACAAGAATATGTGAAAAATCAGCATTACCGGATTGTACAAAATTAATGAg                                                                                                                         <  1:203576/251‑1 (MQ=255)                                                                                              cTTTTTCACCTAGCTCACGACAGTATTCTCCGGCAGACCAACGTTTTACCGCTTTAAAAATTTGAGAACCAATGTCGTTTCCCTTAAAAGGTTCTGCACAGTAATGCACTTTAATCACCATTTTTGAACAAATTTGTTCATAATGTGCAGATTCATCAATATTTAGAAAGCGCCCCCATCGGCTAATGTCATAAACAAGAATATGTGAAAAATCAGCATTACCGGAt                                                                                                                                          >  1:638189/1‑227 (MQ=255)                                                                                                 tttCACCTAGCTCACGACAGTATTCTCCGGCAGACCAACGTTTTACCGCTTTAAAAATTTGAGAACCAATGTCGTTTCCCTTAAAAGGTTCTGCACAGTAATGCACTTTAATCACCATTTTTGAACAAATTTGTTCATAATGTGCAGATTCATCAATATTTAGAAAGCGCCCCCATCGGCTAATGTCATAAACAAGAATATGTGAAAAATCAGCATTACCGGATTGTACAAAATTAATGAGTGATAAAAAc                                                                                                               <  1:659287/251‑1 (MQ=255)                                                                                                      ccTAGCTCACGACAGTATTCTCCGGCAGACCAACGTTTTACCGCTTTAAAAATTTGAGAACCAATGTCGTTTCCCTTAAAAGGTTCTGCACAGTAATGCACTTTAATCACCATTTTTGAACAAATTTGTTCATAATGTGCAGATTCATCAATATTTAGAAAGCGCCCCCATCGGCTAATGTCATAAACAAGAATATGTGAAAAATCAGCATTACCGGATTGTACAAAATTAATGAGTGATAAAAACTGAgg                                                                                                          >  1:282831/1‑251 (MQ=255)                                                                                                          gCTCACGACAGTATTCTCCGGCAGACCAACGTTTTACCGCTTTAAAAATTTGAGAACCAATGTCGTTTCCCTTAAAAGGTTCTGCACAGTAATGCACTTTAATCACCATTTTTGAACAAATTTGTTCATAATGTGCAGATTCATCAATATTTAGAAAGCGCCCCCATCGGCTAATGTCATAAACAAGAATATGTGAAAAATCAGCATTACCGGATTGTACAAAATTAATGAGTGATAAAAACTGAGGACGt                                                                                                      >  1:764554/1‑251 (MQ=255)                                                                                                          gCTCACGACAGTATTCTCCGGCAGACCAACGTTTTACCGCTTTAAAAATTTGAGAACCAATGTCGTTTCCCTTAAAAGGTTCTGCACAGTAATGCACTTTAATCACCATTTTTGAACAAATTTGTTCATAATGTGCAGATTCATCAATATTTAGAAAGCGCCCCCATCGGCTAATGTCATAAACAAGAATATGTGAAAAATCAGCATTACCGGATTGTACAAAATTAATGAGTGATAAAAACTGAGGACGt                                                                                                      >  1:528861/1‑251 (MQ=255)                                                                                                             cACGACAGTATTCTCCGGCAGACCAACGTTTTACCGCTTTAAAAATTTGAGAACCAATGTCGTTTCCCTTAAAAGGTTCTGCACAGTAATGCACTTTAATCACCATTTTTGAACAAATTTGTTCATAATGTGc                                                                                                                                                                                                                         >  1:667011/1‑133 (MQ=255)                                                                                                               cGACAGTATTCTCCGGCAGACCAACGTTTTACCGCTTTAAAAATTTGAGAACCAATGTCGTTTCCCTTAAAAGGTTCTGCACAGTAATGCACTTTAATCACCATTTTTGAACAAATTTGTTCATAATGTGCAGATTCATCAATATTTAGAAAGCGCCCCCATCGGCTAATGTCATAAACAAGAATATGTGAAAAATCAGCATTACCGGATTGTACAAAATTAATGAGTGATAAAAAc                                                                                                               >  1:275004/1‑237 (MQ=255)                                                                                                                 aCAGTATTCTCCGGCAGACCAACGTTTTACCGCTTTAAAAATTTGAGAACCAATGTCGTTTCCCTTAAAAGGTTCTGCACAGTAATGCACTTTAATCACCATTTTTGAACAAATTTGTTCATAATGTGCAGATTCATCAATATTTAGAAAGCGCCCCCATCGGCTAATGTCATAAACAAGAATATGTGAAAAATCAGCATTACCGGATTGTACAAAATTAATGAGTGATAAAAACTGAGGACGttttt                                                                                                  >  1:209106/1‑248 (MQ=255)                                                                                                                    gTATTCTCCGGCAGACCAACGTTTTACCGCTTTAAAAATTTGAGAACCAATGTCGTTTCCCTTAAAAGGTTCTGCACAGTAATGCACTTTAATCACCATTTTTGAACAAATTTGTTCATAATGTGCAGATTCATCAATATTTAGAAAGCGCCCCCATCGGCTAATGTCATAAACAAGAATATGTGAAAAATCAGCATTACCGGATTGTACAAAATTAATGAGTGATAAAAACTGAGGACGTTTTTCTgcg                                                                                             >  1:879735/1‑250 (MQ=255)                                                                                                                      aTTCTCCGGCAGACCAACGTTTTACCGCTTTAAAAATTTGAGAACCAATGTCGTTTCCCTTAAAAGGTTCTCCACAGTAATGCACTTTAATCACCATTTTTGAACAAATTTGTTCATAATGTGCAGATTCATCAATATTTAGAAAGCGCCCCCATCGGCTAATGTCATAAACAAGAATATGTGAAAAATCAGCATTACCGGATTGTACAAAATTAATGAGTGATAAAAACTGAGGACGTTTTTCTGCGCtt                                                                                          >  1:403762/1‑251 (MQ=255)                                                                                                                       ttCTCCGGCAGACCAACGTTTTACCGCTTTAAAAATTTGAGAACCAATGTCGTTTCCCTTAAAAGGTTCTGCACAGTAATGCACTTTAATCACCATTTTTGAACAAATTTGTTCATAATGTGCAGATTCATCAATATTTAGAAAGCGCCCCCATCGGCTAATGTCATAAACAAGAATATGTGAAAAATCAGCATTACCGGATTGTACAAAATTAATGAGTGATAAAAACTGAGGACGTTTTTCTGCGCTTa                                                                                         >  1:476611/1‑251 (MQ=255)                                                                                                                          tcCGGCAGACCAACGTTTTACCGCTTTAAAAATTTGAGAACCAATGTCGTTTCCCTTAAAAGGTTCTGCACAGTAATGCACTTTAATCACCATTTTTGAACAAATTTGTTCATAATGTGCAGATTCATCAATATTTAGAAAGCGCCCCCATCGGCTAATGTCATAAACAAGAATATGTGAAAAATCAGCATTACCGGATTGTACAAAATTAATGAGTGATAAAAACTGAGGACGTTTTTCTGCGCTTAATc                                                                                      <  1:203859/251‑1 (MQ=255)                                                                                                                                 gACCAACGTTTTACCGCTTTAAAAATTTGAGAACCAATGTCGTTTCCCTTAAAAGGTTCTGCACAGTAATGCACTTTAATCACCATTTTTGAACAAATTTGTTCATAATGTGCAGATTCATCAATATTTAGAAAGCGCCCCCATCGGCTAATGTCATAAACAAGAATATGTGAAAAATCAGCATTACCGGATTGTACAAAATTAATGAGTGATAAAAAc                                                                                                               <  1:198100/219‑1 (MQ=255)                                                                                                                                 gACCAACGTTTTACCGCTTTAAAAATTTGAGAACCAATGTCGTTTCCCTTAAAAGGTTCTGCACAGTAATGCACTTTAATCACCATTTTTGAACAAATTTGTTCATAATGTGCAGATTCATCAATATTTAGAAAGCGCCCCCATCGGCTAATGTCATAAACAAGAATATGTGAAAAATCAGCATTACCGGATTGTACAAAATTAATGAGTGATAAAAACTGAGGACGTTTTTCTGCGCTTAATCCACTc                                                                                 >  1:818228/1‑249 (MQ=255)                                                                                                                                      aCGTTTTACCGCTTTAAAAATTTGAGAACCAATGTCGTTTCCCTTAAAAGGTTCTGCACAGTAATGCACTTTAATCACCATTTTTGAACAAATTTGTTCATAATGTGCAGATTCATCAATATTTAGAAAGCGCCCCCATCGGCTAATGt                                                                                                                                                                                >  1:461207/1‑149 (MQ=255)                                                                                                                                      aCGTTTTACCGCTTTAAAAATTTGAGAACCAATGTCGTTTCCCTTAAAAGGTTCTGCACAGTAATGCACTTTAATCACCATTTTTGAACAAATTTGTTCATAATGTGCAGATTCATCAATATTTAGAAAGCGCCCCCATCGGCTAATGTCATAAACAAGAATATGTGAAAAATCAGCATTACCGGATTg                                                                                                                                        >  1:657243/1‑189 (MQ=255)                                                                                                                                      aCGTTTTACCGCTTTAAAAATTTGAGAACCAATGTCGTTTCCCTTAAAAGGTTCTGCACAGTAATGCACTTTAATCACCATTTTTGAACAAATTTGTTCATAATGTGCAGATTCATCAATATTTAGAAAGCGCCCCCATCGGCTAATGTCATAAACAAGAATATGTGAAAAATCAGCATTACCGGATTGTACAAAATTAATGAGTGATAAAAAc                                                                                                               <  1:392833/214‑1 (MQ=255)                                                                                                                                      aCGTTTTACCGCTTTAAAAATTTGAGAACCAATGTCGTTTCCCTTAAAAGGTTCTGCACAGTAATGCACTTTAATCACCATTTTTGAACAAATTTGTTCATAATGTGCAGATTCATCAATATTTAGAAAGCGCCCCCATCGGCTAATGTCATAAACAAGAATATGTGAAAAATCAGCATTACCGGATTGTACAAAATTAATGAGTGATAAAAACTGAGGACGTTTTTCTGCGCTTAATCCACTCCTTcc                                                                            >  1:7515/1‑249 (MQ=255)                                                                                                                                       cGTTTTACCGCTTTAAAAATTTGAGAACCAATGTCGTTTCCCTTAAAAGGTTCTGCACAGTAATGCACTTTAATCACCATTTTTGAACAAATTTGTTCATAATGTGCAGATTCATCAATATTTAGAAAGCGCCCCCATCGGCTAATGTCATAAACAAGAATATGTGAAAAATCAGCATTACCGGATTGTACAAAATTAATGAGTGATAAAAACTGAGGACGTTTTTCTGCGCTTAATCCACTCCTTCCCt                                                                          >  1:663562/1‑250 (MQ=255)                                                                                                                                       cGTTTTACCGCTTTAAAAATTTGAGAACCAATGTCGTTTCCCTTAAAAGGTTCTGCACAGTAATGCACTTTAATCACCATTTTTGAACAAATTTGTTCATAATGTGCAGATTCATCAATATTTAGAAAGCGCCCCCATCGGCTAATGTCATAAACAAGAATATGTGAAAAATCAGCATTACCGGATTGTACAAAATTAATGAGTGATAAAAACTGAGGACGTTTTTCTGCGCTTAATCCACTCCTTCCCTc                                                                         <  1:508809/251‑1 (MQ=255)                                                                                                                                               cGCTTTAAAAATTTGAGAACCAATGTCGTTTCCCTTAAAAGGTTCTGCACAGTAATGCACTTTAATCACCATTTTTGAACAAATTTGTTCATAATGTGCAGATTCATCAATATTTAGAAAGCGCCCCCATCGGCTAATGTCATAAACAAGAATATGTGAAAAATCAGCATTACCGGATTGTACAAAATTAATGAGTGATAAAAACTGAGGACGTTTTTCTGCGCTTAATCCACTCCTTCCCTCATCTAGGt                                                                 <  1:54510/251‑1 (MQ=255)                                                                                                                                                 cTTTAAAAATTTGAGAACCAATGTCGTTTCCCTTAAAAGGTTCTGCACAGTAATGCACTTTAATCACCATTTTTGAACAAATTTGTTCATAATGTGCAGATTCATCAATATTTAGAAAGCGCCCCCATCGGCTAATGTCATAAACAAGAATATGTGAAAAATCAGCATTACCGGATTGTACAAAATTAATGAGTGATAAAAACTGAGGACGTTTTTCTGCGCTTAATCCACTCCTTCCCTCATCTAgg                                                                  >  1:852361/1‑248 (MQ=255)                                                                                                                                                    tAAAAATTTGAGAACCAATGTCGTTTCCCTTAAAAGGTTCTGCACAGTAATGCACTTTAATCACCATTTTTGAACAAATTTGTTCATAATGTGCAGATTCATCAATATTTAGAAAGCGCCCCCATCGGCTAATGTCATAAACAAGAATATGTGAAAAATCAGCATTACCGGATTGTACAAAATTAATGAGTGATAAAAACTGAGGACGTTTTTCTGCGCTTAATCCACTCCTTCCCTCATCTAGGTATTcc                                                            <  1:191066/251‑1 (MQ=255)                                                                                                                                                                aaCCAATGTCGTTTCCCTTAAAAGGTTCTGCACAGTAATGCACTTTAATCACCATTTTTGAACAAATTTGTTCATAATGTGCAGATTCATCAATATTTAGAAAGCGCCCCCATCGGCTAATGTCATAAACAAGAATATGTGAAAAATCAGCATTACCGGATTGTACAAAATTAATGAGTGATAAAAACTGAGGACGTTTTTCTGCGCTTAATCCACTCCTTCCCTCATCTAGGTATTCCCTAGTGAGtata                                                <  1:655353/251‑1 (MQ=255)                                                                                                                                                                 aCCAATGTCGTTTCCCTTAAAAGGTTCTGCACAGTAATGCACTTTAATCACCATTTTTGAACAAATTTGTTCATAATGTGCAGATTCATCAATATTTAGAAAGCGCCCCCATCGGCTAATGTCATAAACAAGAATATGTGAAAAATCAGCATTACCGGATTGTACAAAATTAATGAGTGATAAAAACTGAGGACGTTTTTCTGCGCTTAATCCACTCCTTCCCTCATCTAGGTATTCCCTAGTGAGTATaa                                               >  1:182586/1‑251 (MQ=255)                                                                                                                                                                  ccAATGTCGTTTCCCTTAAAAGGTTCTGCACAGTAATGCACTTTAATCACCATTTTTGAACAAATTTGTTCATAATGTGCAGATTCATCAATATTTAGAAAGCGCCCCCATCGGCTAATGTCATAAACAAGAATATGTGAAAAATCAGCATTACCGGATTGTACAAAAt                                                                                                                                >  1:301291/1‑169 (MQ=255)                                                                                                                                                                  ccAATGTCGTTTCCCTTAAAAGGTTCTGCACAGTAATGCACTTTAATCACCATTTTTGAACAAATTTGTTCATAATGTGCAGATTCATCAATATTTAGAAAGCGCCCCCATCGGCTAATGTCATAAACAAGAATATGTGAAAAATCAGCATTACCGGATTGTACAAAATTAATGAGTGATAAAAACTGAGGACGTTTTTCTgcgc                                                                                            >  1:689404/1‑205 (MQ=255)                                                                                                                                                                  ccAATGTCGTTTCCCTTAAAAGGTTCTGCACAGTAATGCACTTTAATCACCATTTTTGAACAAATTTGTTCATAATGTGCAGATTCATCAATATTTAGAAAGCGCCCCCATCGGCTAATGTCATAAACAAGAATATGTGAAAAATCAGCATTACCGGATTGTACAAAATTAATGAGTGATAAAAACTGAGGACGTTTTTCTGCGCTTAATcc                                                                                     >  1:582871/1‑212 (MQ=255)                                                                                                                                                                  ccAATGTCGTTTCCCTTAAAAGGTTCTGCACAGTAATGCACTTTAATCACCATTTTTGAACAAATTTGTTCATAATGTGCAGATTCATCAATA‑TTAGAAAGCGCCCCCATCGGCTAATGTCATAAACAAGAATATGTGAAAAATCAGCATTACCGGATTGTACAAAATTAATg                                                                                                                           <  1:262854/173‑1 (MQ=255)                                                                                                                                                                    aaTGTCGTTTCCCTTAAAAGGTTCTGCACAGTAATGCACTTTAATCACCATTTTTGAACAAATTTGTTCATAATGTGCAGATTCATCAATATTTAGAAAGCGCCCCCATCGGCTAATGTCATAAACAAGAATATGTGAAAAATCAGCATTACCGGATTGTACAAAATTAATGAGTGATAAAAACTGAGGACGTTTTTCTGCGCTTAATCCACTCCTTCCCTCATCTAGGTATTCCCTAGTGAGTATAAGcc                                            <  1:674090/251‑1 (MQ=255)                                                                                                                                                                           tttCCCTTAAAAGGTTCTGCACAGTAATGCACTTTAATCACCATTTTTGAACAAATTTGTTCATAATGTGCAGATTCATCAATATTTAGAAAGCGCCCCCATCGGCTAATGTCATAAACAAGAATATGTGAAAAATCAGCATTACCGGATTGTACAAAATTAATGAGTGATAAAAACTGAGGACGTTTTTCTGCGCTTAATCCACTCCTTCCCTCATCTAGGTATTCCCTAGTGAGTATAAGCCCTTGTTc                                     <  1:898734/251‑1 (MQ=255)                                                                                                                                                                           tttCCCTTAAAAGGTGCTGCACAGTAATGCACTTTAATCACCATTTTTGAACAAATTTGTTCATAATGTGCAGATTCATCAATATTTAGAAAGCGCCCCCATCGGCTAATGTCATAAACAAGAATATGTGAAAAATCAGCATTACCGGATTGTACAAAATTAATGAGTGATAAAAACTGAGGACGTTTTTCTGCGCTTAATCCACTCCTTCCCTCATCTAGGTATTCCCTAGTGAGTATAAGCCCGTGTTc                                     <  1:464632/251‑1 (MQ=255)                                                                                                                                                                              cccTTAAAAGGTTCTGCACAGTAATGCACTTTAATCACCATTTTTGAACAAATTTGTTCATAATGTGCAGATTCATCAATATTTAGAAAGCGCCCCCATCGGCTAATGt                                                                                                                                                                                >  1:615310/1‑109 (MQ=255)                                                                                                                                                                              cccTTAAAAGGTTCTGCACAGTAATGCACTTTAATCACCATTTTTGAACAAATTTGTTCATAATGTGCAGATTCATCAATATTTAGAAAGCGCCCCCATCGGCTAATGTCATAAACAAGAATATGTGAAAAATCAGCATTACCGGATTGTACAAAATTAATGAGTGATAAAAACTGAGGACGTTTTTCTGCGCTTAATCCACTCCTTCCCTc                                                                         >  1:146956/1‑212 (MQ=255)                                                                                                                                                                                cTTAAAAGGTTCTGCACAGTAATGCACTTTAATCACCATTTTTGAACAAATTTGTTCATAATGTGCAGATTCATCAATATTTAGAAAGCGCCCCCATCGGCTAATGTCATAAACAAGAATATGTGAAAAATCAGCATTACCGGATTGTACAAAATTAATGAGTGATAAAAACTGAGGACGTTTTTCTGCGCTTAATCCACTCCTTCCCTCATCTAGGTATTCCCTAGTGAGTATAAGCCCTTGTTc                                     >  1:484521/1‑246 (MQ=255)                                                                                                                                                                                  tAAAAGGTTCTGCACAGTAATGCACTTTAATCACCATTTTTGAACAAATTTGTTCATAATGTGCAGATTCATCAATATTTAGAAAGCGCCCCCATCGGCTAATGTCATAAACAAGAATATGTGAAAAATCAGCATTACCGGATTGTACAAAATTAATGAGTGATAAAAACTGAGGACGTTTTTCTGCGCTTAATCCACTCCTTCCCTCATCTAGGTATTCCCTAGTGAGTATAAGcc                                            >  1:625960/1‑237 (MQ=255)                                                                                                                                                                                  tAAAAGGTTCTGCACAGTAATGCACTTTAATCACCATTTTTGAACAAATTTGTTCATAATGTGCAGATTCATCAATATTTAGAAAGCGCCCCCATCGGCTAATGTCATAAACAAGAATATGTGAAAAATCAGCATTACCGGATTGTACAAAATTAATGAGTGATAAAAACTGAGGACGTTTTTCTGCGCTTAATCCACTCCTTCCCTCATCTAGGTATTCCCTAGTGAGTATAAGcc                                            >  1:482660/1‑237 (MQ=255)                                                                                                                                                                                       ggTTCTGCACAGTAATGCACTTTAATCACCATTTTTGAACAAATTTGTTCATAATGTGCAGATTCATCAATATTTAGAAAGCGCCCCCATCGGCTAATGTCATAAACAAGAATATGTGAAAAATCAGCATTACCGGATTGTACAAAATTAATGAGTGATAAAAACTg                                                                                                             <  1:487558/167‑1 (MQ=255)                                                                                                                                                                                       ggTTCTGCACAGTAATGCACTTTAATCACCATTTTTGAACAAATTTGTTCATAATGTGCAGATTCATCAATATTTAGAAAGCGCCCCCATCGGCTAATGTCATAAACAAGAATATGTGAAAAATCAGCATTACCGGATTGTACAAAATTAATGAGTGATAAAAACTGAGGACGTTTTTCTGCGCTTAATCCACTCCTTCCCTCATCTAGGTATTCCCTAGTGAGTATAAGCCCTTGTTCCTTAGCATAttt                         >  1:241916/1‑251 (MQ=255)                                                                                                                                                                                        gTTCTGCACAGTAATGCACTTTAATCACCATTTTTGAACAAATTTGTTCATAATGTGCAGATTCATCAATATTTAGAAAGCGCCCCCATCGGCTAATGTCATAAACAAGAATATGTGAAAAATCAGCATTACCGGATTGTACAAAATTAATGAGTGATAAAAACTGAGGACGTTTTTCTGCGCTTAATCCACTCCTTCCCTCATCTAGGTATTCCCTAGTGAGTATAAGCCCTTGTTc                                     >  1:667348/1‑238 (MQ=255)                                                                                                                                                                                           cTGCACAGTAATGCACTTTAATCACCATTTTTGAACAAATTTGTTCATAATGTGCAGATTCATCAATATTTAGAAAGCGCCCCCATCGGCTAATGTCATAAACAAGAATATGTGAAAAATCAGCATTACCGGATTGTAc                                                                                                                                     >  1:301282/1‑139 (MQ=255)                                                                                                                                                                                           cTGCACAGTAATGCACTTTAATCACCATTTTTGAACAAATTTGTTCATAATGTGCAGATTCATCAATATTTAGAAAGCGCCCCCATCGGCTAATGTCATAAACAAGAATATGTGAAAAATCAGCATTACCGGATTGTACAAAATTAATGAGTGATAAAAACTGAGGACGTTTTTCTGCGCTTAATCCACTCCTTCCCTCATCTAGGTATTCCCTAGTGAGTATAAGcc                                            >  1:640873/1‑228 (MQ=255)                                                                                                                                                                                           cTGCACAGTAATGCACTTTAATCACCATTTTTGAACAAATTTGTTCATAATGTGCAGATTCATCAATATTTAGAAAGCGCCCCCATCGGCTAATGTCATAAACAAGAATATGTGAAAAATCAGCATTACCGGATTGTACAAAATTAATGAGTGATAAAAACTGAGGACGTTTTTCTGCGCTTAATCCACTCCTTCCCTCATCTAGGTATTCCCTAGTGAGTATAAGCCCTTGTTCCTTAGCATATTTTtga                     >  1:344605/1‑251 (MQ=255)                                                                                                                                                                                            tGCACAGTAATGCACTTTAATCACCATTTTTGAACAAATTTGTTCATAATGTGCAGATTCATCAATATTTAGAAAGCGCCCCCATCGGCTAATGTCATAAACAAGAATATGTGAAAAATCAGCATTACCGGATTGTACAAAATTAATGAGTGATAAAAACTGAGGACGTTTTTCTGCGCTTAATCCACTCCTTCCCTCATCTAGGTATTCCCTAGTGAGTATAAGCCCTTGTTCCTTAGCATATTTTtgaa                    <  1:457715/251‑1 (MQ=255)                                                                                                                                                                                             gCACAGTAATGCACTTTAATCACCATTTTTGAACAAATTTGTTCATAATGTGCAGATTCATCAATATTTAGAAAGCGCCCCCATCGGCTAATGTCATAAACAAGAATATGTGAAAAATCAGCATTACCGGATTGTACAAAATTAATGAGTGATAAAAACTGAGGACGTTTTTCTGCGCTTAATCCACTCCTTCCCTCATCTAGGTATTCCCTAGTGAGTATAAGCCCTTGTTCCTTAGCata                            >  1:730757/1‑242 (MQ=255)                                                                                                                                                                                                caGTAATGCACTTTAATCACCATTTTTGAACAAATTTGTTCATAATGTGCAGATTCATCAATATTTAGAAAGCGCCCCCATCGGCTAATGTCATAAACAAGAATATGTGAAAAATCAGCATTACCGGATTGTACAAAATTAATGAGTGATAAAAACTGAGGACGTTTTTCTGCGCTTAATCCACTCCTTCCCTCATCTAGGTATTCCCTAGTGAGTATAAGcc                                            >  1:680721/1‑223 (MQ=255)                                                                                                                                                                                                  gTAATGCACTTTAATCACCATTTTTGAACAAATTTGTTCATAATGTGCAGATTCATCAATATTTAGAAAGCGCCCCCATCGGCTAATGTCATAAACAAGaa                                                                                                                                                                    <  1:349912/101‑1 (MQ=255)                                                                                                                                                                                                  gTAATGCACTTTAATCACCATTTTTGAACAAATTTGTTCATAATGTGCAGATTCATCAATATTTAGAAAGCGCCCCCATCGGCTAATGTCATAAACAAGAATATGTGAAAAATCAGCATTACCGGATTGTACAAAATTAATGAGTGATaaaa                                                                                                                 >  1:341810/1‑152 (MQ=255)                                                                                                                                                                                                  gTAATGCACTTTAATCACCATTTTTGAACAAATTTGTTCATAATGTGCAGATTCATCAATATTTAGAAAGCGCCCCCATCGGCTAATGTCATAAACAAGAATATGTGAAAAATCAGCATTACCGGATTGTACAAAATTAATGAGTGATAAAAACTGAGGACGtttt                                                                                                   >  1:413721/1‑166 (MQ=255)                                                                                                                                                                                                  gTAATGCACTTTAATCACCATTTTTGAACAAATTTGTTCATAATGTGCAGATTCATCAATATTTAGAAAGCGCCCCCATCGGCTAATGTCATAAACAAGAATATGTGAAAAATCAGCATTACCGGATTGTACAAAATTAATGAGTGATAAAAACTGAGGACGTTTTTc                                                                                                 <  1:185397/168‑1 (MQ=255)                                                                                                                                                                                                  gTAATGCACTTTAATCACCATTTTTGAACAAATTTGTTCATAATGTGCAGATTCATCAATATTTAGAAAGCGCCCCCATCGGCTAATGTCATAAACAAGAATATGTGAAAAATCAGCATTACCGGATTGTACAAAATTAATGAGTGATAAAAACTGAGGACGTTTTTCTGCGCTTAATCCACTCCTTCCCTCATCTAGGt                                                                 <  1:714482/200‑1 (MQ=255)                                                                                                                                                                                                  gTAATGCACTTTAATCACCATTTTTGAACAAATTTGTTCATAATGTGCAGATTCATCAATATTTAGAAAGCGCCCCCATCGGCTAATGTCATAAACAAGAATATGTGAAAAATCAGCATTACCGGATTGTACAAAATTAATGAGTGATAAAAACTGAGGACGTTTTTCTGCGCTTAATCCACTCCTTCCCTCATCTAGGTATTCCCTAGTg                                                      >  1:118333/1‑211 (MQ=255)                                                                                                                                                                                                  gTAATGCACTTTAATCACCATTTTTGAACAAATTTGTTCATAATGTGCAGATTCATCAATATTTAGAAAGCGCCCCCATCGGCTAATGTCATAAACAAGAATATGTGAAAAATCAGCATTACCGGATTGTACAAAATTAATGAGTGATAAAAACTGAGGACGTTTTTCTGCGCTTAATCCACTCCTTCCCTCATCTAGGTATTCCCTAGTGAGTATAAGCCCTTGTTc                                     <  1:136885/228‑1 (MQ=255)                                                                                                                                                                                                  gTAATGCACTTTAATCACCATTTTTGAACAAATTTGTTCATAATGTGCAGATTCATCAATATTTAGAAAGCGCCCCCATCGGCTAATGTCATAAACAAGAATATGTGAAAAATCAGCATTACCGGATTGTACAAAATTAATGAGTGATAAAAACTGAGGACGTTTTTCTGCGCTTAATCCACTCCTTCCCTCATCTAGGTATTCCCTAGTGAGTATAAGCCCTTGTTc                                     <  1:882974/228‑1 (MQ=255)                                                                                                                                                                                                  gTAATGCACTTTAATCACCATTTTTGAACAAATTTGTTCATAATGTGCAGATTCATCAATATTTAGAAAGCGCCCCCATCGGCTAATGTCATAAACAAGAATATGTGAAAAATCAGCATTACCGGATTGTACAAAATTAATGAGTGATAAAAACTGAGGACGTTTTTCTGCGCTTAATCCACTCCTTCCCTCATCTAGGTATTCCCTAGTGAGTATAAGCCCTTGTTCCTTAGCat                             <  1:777094/236‑1 (MQ=255)                                                                                                                                                                                                  gTAATGCACTTTAATCACCATTTTTGAACAAATTTGTTCATAATGTGCAGATTCATCAATATTTAGAAAGCGCCCCCATCGGCTAATGTCATAAACAAGAATATGTGAAAAATCAGCATTACCGGATTGTACAAAATTAATGAGTGATAAAAACTGAGGACGTTTTTCTGCGCTTAATCCACTCCTTCCCTCATCTAGGTATTCCCTAGTGAGTATAAGCCCTTGTTCCTTAGCATAtttt                        <  1:850108/241‑1 (MQ=255)                                                                                                                                                                                                  gTAATGCACTTTAATCACCATTTTTGAACAAATTTGTTCATAATGTGCAGATTCATCAATATTTAGAAAGCGCCCCCATCGGCTAATGTCATAAACAAGAATATGTGAAAAATCAGCATTACCGGATTGTACAAAATTAATGAGTGATAAAAACTGAGGACGTTTTTCTGCGCTTAATCCACTCCTTCCCTCATCTAGGTATTCCCTAGTGAGTATAAGCCCTTGTTCCTTAGCATATTTTTGAATGAAg               >  1:300313/1‑250 (MQ=255)                                                                                                                                                                                                  gTAATGCACTTTAATCACCATTTTTGAACAAATTTGTTCATAATGTGCAGATTCATCAATATTTAGAAAGCGCCCCCATCGGCTAATGTCATAAACAAGAATATGTGAAAAATCAGCATTACCGGATTGTACAAAATTAATGAGTGATAAAAACTGAGGACGTTTTTCTGCGCTTAATCCACTCCTTCCCTCATCTAGGTATTCCCTAGTGAGTATAAGCCCTTGTTCCTTAGCATATTTTTGAATGAAg               >  1:744010/1‑250 (MQ=255)                                                                                                                                                                                                  gTAATGCACTTTAATCACCATTTTTGAACAAATTTGTTCATAATGTGCAGATTCATCAATATTTAGAAAGCGCCCCCATCGGCTAATGTCATAAACAAGAATATGTGAAAAATCAGCATTACCGGATTGTACAAAATTAATGAGTGATAAAAACTGAGGACGTTTTTCTGCGCTTAATCCACTCCTTCCCTCATCTAGGTATTCCCTAGTGAGTATAAGCCCTTGTTCCTTAGCATATTTTTGAATGAAg               >  1:605601/1‑250 (MQ=255)                                                                                                                                                                                                  gTAATGCACTTTAATCACCATTTTTGAACAAATTTGTTCATAATGTGCAGATTCATCAATATTTAGAAAGCGCCCCCATCGGCTAATGTCATAAACAAGAATATGTGAAAAATCAGCATTACCGGATTGTACAAAATTAATGAGTGATAAAAACTGAGGACGTTTTTCTACGCTTAATCCACTCCTTccc                                                                           >  1:445907/1‑190 (MQ=255)                                                                                                                                                                                                  gTAATGCACTTTAATCACCATTTTTGAACAAATTTGTTCATAATGTGCAGATTCATCAATATTTAGAAAGCGCCCCCATCGGCTAATGTCATAAACAAGAATATGTGAAAAATCAGCATTACCGGATTGTACAAAATTAATGAGTGATAAAAACTGAGGACGTTTTTCCGCGCTTAATCCACTCCTTCCCTCATCTAGGTATTCCCTAGTGAGTATAAGCCCTTGTTCCTTAGCATATTTTTGAATGAAg               >  1:160646/1‑250 (MQ=255)                                                                                                                                                                                                          cTTTAATCACCATTTTTGAACAAATTTGTTCATAATGTGCAGATTCATCAATATTTAGAAAGCGCCCCCATCGGCTAATGTCATAAACAAGAATATGTGAAAAATCAGCATTACCGGATTGTACAAAATTAATGAGTGATAAAAACTGAGGACGTTTTTc                                                                                                 <  1:890594/160‑1 (MQ=255)                                                                                                                                                                                                            ttAATCACCATTTTTGAACAAATTTGTTCATAATGTGCAGATTCATCAATATTTAGAAAGCGCCCCCATCGGCTAATGTCATAAACAAGAATATGTGAAAAATCAGCATTACCGGATTGTACAAAATTAATGAGTGATAAAAACTGAGGACGTTTTTCTGCGCTTAATCCACTCCTTCCCTCATCTAGGTATTCCCTAGTGAGTATAAGCCCTTGTTCCTTAGCATATTTTTGAATGAAGCGACGTTGtat    <  1:294120/251‑1 (MQ=255)                                                                                                                                                                                                            ttAATCACCATTTTTGAACAAATTTGTTCATAATGTGCAGATTCATCAATATTTAGAAAGCGCCCCCATCGGCTAATGTCATAAACAAGAATATGTGAAAAATCAGCATTACCGGATTGTACAAAATTAATGAGTGATAAAAACTGAGGACGTTTTTCTGCGCTTAATCCACTCCTTCCCTCATCTAGGTATTCCCTAGTGAGTATAAGCCCTTGTTCCTTAGCATATTTTTGAATGAAGCGACGTTGtat    <  1:261974/251‑1 (MQ=255)                                                                                                                                                                                                              aaTCACCATTTTTGAACAAATTTGTTCATAATGTGCAGATTCATCAATATTTAGAAAGCGCCCCCATCGGCTAATGTCATAAACAAGAATATGTGAAAAATCAGCATTACCGGATTGTACAAAATTAATGAGTGATAAAAACTGAGGACGTTTTTCTGCGCTTAATCCACTCCTTCCCTCATCTAGGTATTCCCTAGTGAGTATAAGCCCTTGTTCCTTAGCATATTTTTGAATGAAGCGACGTTGTatat  <  1:585937/251‑1 (MQ=255)                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                       CATCAGCACTTAATAATAGGCGTCTTAACCCAAATCCAGCTGGTCCACCTTGACGAAATCCGCGCTCAATCAAATTCTTCTGCCCATTAAAAACTTTTTCACCTAGCTCACGACAGTATTCTCCGGCAGACCAACGTTTTACCGCTTTAAAAATTTGAGAACCAATGTCGTTTCCCTTAAAAGGTTCTGCACAGTAATGCACTTTAATCCCCATTTTTGAACAAATTTGTTCATAATGTGCAGATTCATCAATATTTAGAAAGCGCCCCCATCGGCTAATGTCATAAACAAGAATATGTGAAAAATCAGCATTACCGGATTGTACAAAATTAATGAGTGATAAAAACTGAGGACGTTTTTCTGCGCTTAATCCACTCCTTCCCTCATCTAGGTATTCCCTAGTGAGTATAAGCCCTTGTTCCTTAGCATATTTTTGAATGAAGCGACGTTGTATAT  >  1/309328‑309783

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑