Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,979,853	G→T	G758G (GGC→GGA)	unknown ←	RTX toxins determinant A and related Ca2+‑binding proteins

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,979,853	0	G	T	100.0%	105.9 / NA	39	G758G (GGC→GGA)	unknown	RTX toxins determinant A and related Ca2+‑binding proteins
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (10/29);  total (10/29)

TCATTGCCACTTCCACCTCTTAGAATATCGGAACCATTGCCTCCCTCTAAGTGGTCGTCGCCTTCTCCACCGTCAAGATAGTTGTTGCCATATTCACCAAAGAGCCTATCGTTACCGGCACCACCATAGAGCTTATCATTACCTTGGCCGCCATGAATTTCGTCATTCCCATTATCGCCATACAAACGATCATCGCCATCATAACCATAAATCAAATCATCGCCATCGTGACCATGGAAAACATCATTAAATTTAGAACCATAAAATTTATCACGTAGTGTGCTGCCGATAATTTCCTCTACTGAGTTTAATTCATCATGAGCTTTAAAGCCCTTATTCGTATAATAGTAATCTAATTTATAGTTACGATATTCAAGTAGATCGGTAACCTTCCCTCGTTTAGTTTCTTGCTTTGA  >  1/1979677‑1980092                                                                                                                                                                                 |                                                                                                                                                                                                                                                tCATTGCCACTTCCGCCTCTTAGAATATCGGAACCATTCCCCCCTTCTAAATGGTCGTCGCCTTCACCACCGTCTAGATAGTTGTTACCATATTCACCAAAGAGCCTGTCATTGCCGGCGCCACCATAAAGTTTGTCGTTGCCTTTTCCTCCATGGATCTCATCATTACCATTATCTCCATATAAGCGGTCATCACCATCATAACCATAAATCACATCATCACCATCGTGACCATGGAAAACATCATTaaa                                                                                                                                                                       <  1:870761/251‑1 (MQ=255)                 ctctTAGAATATCGGAACCATTCCCCCCTTCTAAATGGTCGTCGCCTTCACCACCGTCTAGATAGTTGTTACCATATTCACCAAAGAGCCTGTCATTGCCGGCGCCACCATAAAGTTTGTCGTTGCCTTTTCCTCCATGGATCTCATCATTACCATTATCTCCATATAAGCGGTCATCACCATCATAACCATAAATCACATCATCACCATCGTGACCATGGAAAACATCATTAAATCTAGAACCATaaaaa                                                                                                                                                       <  1:885681/251‑2 (MQ=255)                  tctTAGAATATCGGAACCATTCCCCCCTTCTAAATGGTCGTCGCCTTCACCACCGTCTAGATAGTTGTTACCATATTCACCAAAGAGCCTGTCATTGCCGGCGCCACCATAAAGTTTGTCGTTGCCTTTTCCTCCATGGATCTCATCATTACCATTATCTCCATATAAGCGGTCATCACCATCATAACCATAAATCACATCATCACCATCGTGACCATGGAAAACATCATTAAAt                                                                                                                                                                      <  1:471351/235‑1 (MQ=255)                  tctTAGAATATCGGAACCATTCCCCCCTTCTAAATGGTCGTCGCCTTCACCACCGTCTAGATAGTTGTTACCATATTCACCAAAGAGCCTGTCATTGCCGGCGCCACCATAAAGTTTGTCGTTGCCTTTTCCTCCATGGATCTCATCATTACCATTATCTCCATATAAGCGGTCATCACCATCATAACCATAAATCACATCATCACCATCGTGACCATGGAAAACATCATTAAATCTAGAACCATaaaaat                                                                                                                                                      >  1:273575/1‑249 (MQ=255)                   ctTAGAATATCGGAACCATTCCCCCCTTCTAAATGGTCGTCGCCTTCACCACCGTCTAGATAGTTGTTACCATATTCACCAAAGAGCCTGTCATTGCCGGCGCCGCCATAAAGTTTGTCGTTGCCTTTTCCTCCATGGATCTCATCATTACCATTATCTCCATATAAGCGGTCATCACCATCATAACCATAAATCACATCATCACCATCGTGACCATGGAAAACATCATTAAATCTAGAACCATaaaaatg                                                                                                                                                     <  1:210238/251‑4 (MQ=255)                                 aCCATTCCCCCCTTCTAAATGGTCGTCGCCTTCACCACCGTCTAGATAGTTGTTACCATATTCACCAAAGAGCCTGTCATTGCCGGCGCCACCATAAAGTTTGTCGTTGCCTTTTCCTCCATGGATCTCATCATTACCATTATCTCCATATAAGCGGTCATCACCATCATAACCATAAATCACATCATCACCATCGTGACCATGGAAAACATCATTAAATCTAGAACCATaaaaatgatcccgtaatttac                                                                                                                                       <  1:693251/251‑18 (MQ=255)                                 aCCATTCCCCCCTTCTAAATGGTCGTCGCCTTCACCACCGTCTAGATAGTTGTTACCATATTCACCAAAGAGCCTGTCATTGCCGGCGCCACCATAAAGTTTGTCGTTGCCTTTTCCTCCATGGATCTCATCATTACCATTATCTCCATATAAGCGGTCATCACCATCATAACCATAAATCACATCATCACCATCGTGACCATGGAAAACATCATTAAATCTAGAACCATaaaaatgatcccgtaatttac                                                                                                                                       <  1:344235/251‑18 (MQ=255)                                       ccccccttctAAATGGTCGTCGCCTTCACCACCGTCTAGATAGTTGTTACCATATTCACCAAAGAGCCTGTCATTGCCGGCGCCACCATAAAGTTTGTCGTTGCCTTTTCCTCCATGGATCTCATCATTACCATTATCTCCATATAAGCGGTCATCACCATCATAACCATAAATCACATCATCACCATCGTGACCATGGAAAACATCATTAAATCTAGAACCATaa                                                                                                                                                          >  1:500519/8‑226 (MQ=255)                                                      gtcgtcGCCTTCACCACCGTCTAGATAGTTGTTACCATATTCACCAAAGAGCCTGTCATTGCCGGCGCCACCATAAAGTTTGTCGTTGCCTTTTCCTCCATGGATCTCATCATTACCATTATCTCCATATAAGCGGTCATCACCATCATAACCATAAATCACATCATCACCATCGTGACCATGGAAAACATCATTAAATCTAGAACCATAAAAATGATCCCGTAATTTACTGCCGATAATTTCCTCTAc                                                                                                                    <  1:420430/249‑1 (MQ=255)                                                          tcgCCTTCACCACCGTCTAGATAGTTGTTACCATATTCACCAAAGAGCCTGTCATTGCCGGCGCCACCATAAAGTTTGTCGTTGCCTTTTCCTCCATGGATCTCATCATTACCATTATCTCCATATAAGCGGTCATCACCATCATAACCATAAATCACATCATCACCATCGTGACCATGGAAAACATCATTAAATCTAGAACCATAAAAATGATCCCGTAATTTACTGCCGATAATTTCCTCTACTGAGt                                                                                                               <  1:191139/250‑1 (MQ=255)                                                            gCCTTCACCACCGTCTAGATAGTTGTTACCATATTCACCAAAGAGCCTGTCATTGCCGGCGCCACCATAAAGTTTGTCGTTGCCTTTTCCTCCATGGATCTCATCATTACCATTATCTCCATATAAGCGGTCATCACCATCATAACCATAAATCACATCATCACCATCGTGACCATGGAAAACATCATTAAATCTAGAACCATAAAAATGATCCCGTAATTTACTGCCGATAATTTCCTCTACTGAGTTTa                                                                                                            >  1:570569/1‑251 (MQ=255)                                                             ccTTCACCACCGTCTAGATAGTTGTTACCATATTCACCAAAGAGCCTGTCATTGCCGGCGCCACCATAAAGTTCGTCGTTGCCTTTTCCTCCATGGATCTCATCATTACCATTATCTCCATATAAGCGGTCATCACCATCATAACCATAAATCACATCATCACCATCGTGACCATGGAAAACATCATTAAATCTAGAACCATAAAAATGATCCCGTAATTTACTGCCGATAATTTCCTCTAc                                                                                                                    <  1:751858/242‑1 (MQ=255)                                                              cTTCACCACCGTCTAGATAGTTGTTACCATATTCACCAAAGAGCCTGTCATTGCCGGCGCCACCATAAAGTTTGTCGTTGCCTTTTCCTCCATGGATCTCATCATTACCATTATCTCCATATAAGCGGTCATCACCATCATAACCATAAATCACATCATCACCATCGTGACCATGGAAAACATCATTAAAtc                                                                                                                                                                     >  1:300832/1‑191 (MQ=255)                                                                tcaccACCGTCTAGATAGTTGTTACCATATTCACCAAAGAGCCTGTCATTGCCGGCGCCACCATAAAGTTTGTCGTTGCCTTTTCCTCCATGGATCTCATCATTACCATTATCTCCATATAAGCGGTCATCACCATCATAACCATAAATCACATCATCACCATCGTGACCATGGAAAACATCATTAAATCTAGAACCATAAAAATGATCCCGTAATTTACTGCCGATAATTTCCTCTACTGAGTTTAATtc                                                                                                        <  1:671199/248‑1 (MQ=255)                                                                tcaccACCGTCTAGATAGTTGTTACCATATTCACCAAAGAGCCTGTCATTGCCGGCGCCACCATAAAGTTTGTCGTTGCCTTTTCCTCCATGGATCTCATCATTACCATTATCTCCATATAAGCGGTCATCACCATCATAACCATAAATCACATCATCACCATCGTGACCATGGAAAACATCATTAAATCTAGAACCATAAAAATGATCCCGTAATTTACTGCCGATAATTTCCTCTACTGAGTTTAATtc                                                                                                        <  1:588716/248‑1 (MQ=255)                                                                      ccGTCTAGATAGTTGTTACCATATTCACCAAAGAGCCTGTCATTGCCGGCGCCACCATAAAGTTTGTCGTTGCCTTTTCCTCCATGGATCTCATCATTACCATTATCTCCATATAAGCGGTCATCACCATCATAACCATAAATCACATCATCACCATCGTGACCATGGAAAACATCATTAAATCTAGAACCATAAAAATGATCCCGTAATTTACTGCCGATAATTTCCTCTACTGAGTTTAATTCATCATg                                                                                                  >  1:332492/1‑251 (MQ=255)                                                                       cGTCTAGATAGTTGTTACCATATTCACCAAAGAGCCTGTCATTGCCGGCGCCACCATAAAGTTTGTCGTTGCCTTTTCCTCCATGGATCTCATCATTACCATTATCTCCATATAAGCGGTCATCACCATCATAACCATAAATCACATCATCACCATCGTGACCATGGAAAACATCATTAAATCTAGAAc                                                                                                                                                               <  1:851471/189‑1 (MQ=255)                                                                                 gttgttACCATATTCACCAAAGAGCCTGTCATTGCCGGCGCCACCATAAAGTTTGTCGTTGCCTTTTCCTCCATGGATCTCATCATTACCATTATCTCCATATAAGCGGTCATCACCATCATAACCATAAATCACATCATCACCATCGTGACCATGGAAAACATCATTAAATCTAGAACCATAAAAATGATCCCGTAATTTACTGCCGATAATTTCCTCTACTg                                                                                                                  <  1:521832/224‑1 (MQ=255)                                                                                   tgttaCCATATTCACCAAAGAGCCTGTCATTGCCGGCGCCACCATAAAGTTTGTCGTTGCCTTTTCCTCCATGGATCTCATCATTACCATTATCTCCATATAAGCGGTCATCACCATCATAACCATAAATCACATCATCACCATCGTGACCATGGAAAACATCATTAAATCTAGAACCATAAAAATGATCCCGTAATTTACTGCCGATAATTTCCTCTACTGAGTTTAATTCATCATGAGCTTTAAAGccc                                                                                     <  1:392856/251‑1 (MQ=255)                                                                                        ccATATTCACCAAAGAGCCTGTCATTGCCGGCGCCACCATAAAGTTTGTCGTTGCCTTTTCCTCCATGGATCTCATCATTATCATTATCTCCATATAAGCGGTCATCACCATCATAACCATAAATCACATCATCACCATCGTGACCATGGAAAACATCATTAAATCTAGAACCATAAAAATGATCCCGTAATTTACTGCCGATAATTTCCTCTACTGAGTTTAATTCATCATGAGCTTTAAAGCCCTTAtt                                                                                <  1:429599/251‑1 (MQ=255)                                                                                        ccATATTCACCAAAGAGCCTGTCATTGCCGGCGCCACCATAAAGTTTGTCGTTGCCTTTTCCTCCATGGATCTCATCATTACCATTATCTCCATATAAGCGGTCATCACCATCATAACCATAAATCACATCATCACCATCGTGACCATGGAAAACATCATTAAATCTAGAACCATAAAAATGATCCCGTAATTTACTGCCGATAATTTCCTCTACTGAGTTTAATTCATCATGAGCTTTAAAGCCCTTAtt                                                                                <  1:185394/251‑1 (MQ=255)                                                                                        ccATATTCACCAAAGAGCCTGTCATTGCCGGCGCCACCATAAAGTTTGTCGTTGCCTTTTCCTCCATGGATCTCATCATTACCATTATCTCCATATAAGCGGTCATCACCATCATAACCATAAATCACATCATCACCATCGTGACCATGGAAAACATCATTAAATCTAGAACCATAAAAATGATCCCGTAATTTACTGCCGATAATTTCCTCTACTGAGTTTAATTCATCATGAGCTTTAAAGCCCTTAtt                                                                                <  1:159658/251‑1 (MQ=255)                                                                                            atTCACCAAAGAGCCTGTCATTGCCGGCGCCACCATAAAGTTTGTCGTTGCCTTTTCCTCCATGGATCTCATCATTACCATTATCTCCATATAAGCGGTCATCACCATCATAACCATAAATCACATCATCACCATCGTGACCATGGAAAACATCATTAAATCTAGAACCATAAAAATGATCCCGTAATTTACTGCCGATAATTTCCTCTACTGAGTTTAATTCATCATGAGc                                                                                               <  1:645074/232‑1 (MQ=255)                                                                                               cACCAAAGAGCCTGTCATTGCCGGCGCCACCATAAAGTTTGTCGTTGCCTTTTCCTCCATGGATCTCATCATTACCATTATCTCCATATAAGCGGTCATCACCATCATAACCATAAATCACATCATCACCATCGTGACCATGGAAAACATCATTAAATCTAGAACCATAAAAATGATCCCGTAATTTACTGCCGATAATTTCCTCTACTGAGTTTAATTCATCATGAGCTTTAAAGCCCTTAtt                                                                                <  1:470858/244‑1 (MQ=255)                                                                                                 ccAAAGAGCCTGTCATTGCCGGCGCCACCATAAAGTTTGTCGTTGCCTTTTCCTCCATGGATCTCATCATTACCATTATCTCCATATAAGCGGTCATCACCATCATAACCATAAATCACATCATCACCATCGTGACCATGGAAAACATCATTAAAtc                                                                                                                                                                     <  1:243468/157‑2 (MQ=255)                                                                                                 ccAAAGAGCCTGTCATTGCCGGCGCCACCATAAAGTTTGTCGTTGCCTTTTCCTCCATGGATCTCATCATTACCATTATCTCCATATAAGCGGTCATCACCATCATAACCATAAATCACATCATCACCATCGTGACCATGGAAAACATCATTAAATCTAGAAc                                                                                                                                                               <  1:2688/163‑1 (MQ=255)                                                                                                 ccAAAGAGCCTGTCATTGCCGGCGCCACCATAAAGTTTGTCGTTGCCTTTTCCTCCATGGATCTCATCATTACCATTATCTCCATATAAGCGGTCATCACCATCATAACCATAAATCACATCATCACCATCGTGACCATGGAAAACATCATTAAATCTAGAACCATAAAAATGATCCCGTAATTTACTGCCGATAATTTCCTCTACTGAGTTTAATTCATCATGAGCTTTAAAGCCCTTATTCGTataata                                                                       >  1:141191/1‑251 (MQ=255)                                                                                                  cAAAGAGCCTGTCATTGCCGGCGCCACCATAAAGTTTGTCGTTGCCTTTTCCTCCATGGATCTCATCATTACCATTATCTCCATATAAGCGGTCATCACCATCATAACCATAAATCACATCATCACCATCGTGACCATGGAAAACATCATTAAATCTAGAACCATAAAAATGATCCCGTAATTTACTGCCGATAATTTCCTCTACTGAGTTTAATTCATCATGAGCTTTAAAGCCCTTATTCGTATAATAg                                                                      <  1:557348/251‑1 (MQ=255)                                                                                                  cAAAGAGCCTGTCATTGCCGGCGCCACCATAAAGTTTGTCGTTGCCTTTTCCTCCATGGATCTCATCATTACCATTATCTCCATATAAGCGGTCATCACCATCATAACCATAAATCACATCATCACCATCGTGACCATGGAAAACATCATTAAATCTAGAACCATAAAAATGATCCCGTAATTTACTGCCGATAATTTCCTCTACTGAGTTTAATTCATCATGAGCTTTAAAGCCCTTATTCGTATAATAg                                                                      <  1:152925/251‑1 (MQ=255)                                                                                                                                                    cctccatggatctcatCATTACCATTATCTCCATATAAGCGGTCATCACCATCATAACCATAAATCACATCATCACCATCGTGACCATGGAAAACATCATTAAATCTAGAACCATAAAAATGATCCCGTAATTTACTGCCGATAATTTCCTCTACTGAGTTTAATTCATCATGAGCTTTAAAGCCCTTATTCGTATAATAGTAATCTAATTTATAGTTACGATATTCAAGTAGATCGGTAACCTTCCCTCg                    >  1:16182/16‑251 (MQ=255)                                                                                                                                                            gatctcatCATTACCATTATCTCCATATAAGCGGTCATCACCATCATAACCATAAATCACATCATCACCATCGTGACCATGGAAAACATCATTAAATCTAGAACCATAAAAATGATCCCGTAATTTACTGCCGATAATTTCCTCTACTGAGTTTAATTCATCATGAGCTTTAAAGCCCTTATTCGTATAATAg                                                                      <  1:807841/186‑1 (MQ=255)                                                                                                                                                            gatctcatCATTACCATTATCTCCATATAAGCGGTCATCACCATCATAACCATAAATCACATCATCACCATCGTGACCATGGAAAACATCATTAAATCTAGAACCATAAAAATGATCCCGTAATTTACTGCCGATAATTTCCTCTACTGAGTTTAATTCATCATGAGCTTTAAAGCCCTTATTCGTATAATAGTAATCTAATTTATAg                                                       >  1:516618/8‑208 (MQ=255)                                                                                                                                                             atctcatCATTACCATTATCTCCATATAAGCGGTCATCACCATCATAACCATAAATCACATCATCACCATCGTGACCATGGAAAACATCATTAAATCTAGAAc                                                                                                                                                               <  1:583420/97‑1 (MQ=255)                                                                                                                                                             atctcatCATTACCATTATCTCCATATAAGCGGTCATCACCATCATAACCATAAATCACATCATCACCATCGTGACCATGGAAAACATCATTAAATCTAGAACCATAAAAATGATCCCGTAATTTACTGCCGATAATTTCCTCTACTGAGTTTAAt                                                                                                          <  1:773700/150‑1 (MQ=255)                                                                                                                                                                 catCATTACCATTATCTCCATATAAGCGGTCATCACCATCATAACCATAAATCACATCATCACCATCGTGACCATGGAAAACATCATTAAATCTAGAACCATAAAAATGATCCCGTAATTTACTGCCGATAATTTCCTCTACTGAGTTTAATTCATCATGAGCTTTAAAGCCCTTATTc                                                                               <  1:282369/177‑1 (MQ=255)                                                                                                                                                                 catCATTACCATTATCTCCATATAAGCGGTCATCACCATCATAACCATAAATCACATCATCACCATCGTGACCATGGAAAACATCATTAAATCTAGAACCATAAAAATGATCCCGTAATTTACTGCCGATAATTTCCTCTACTGAGTTTAATTCATCATGAGCTTTAAAGCCCTTATTCGTATAATAg                                                                      >  1:161063/3‑188 (MQ=255)                                                                                                                                                                  atCATTACCATTATCTCCATATAAGCGGTCATCACCATCATAACCATAAATCACATCATCACCATCGTGACCATGGAAAACATCATTAAATCTAGAAc                                                                                                                                                               <  1:130559/97‑1 (MQ=255)                                                                                                                                                                      ttaccATTATCTCCATATAAGCGGTCATCACCATCATAACCATAAATCACATCATCACCATCGTGACCATGGAAAACATCATTAAATCTAGAACCATAAAAATGATCCCGTAATTTACTGCCGATAATTTCCTCTACTGAGTTTAATTCATCATGAGCTTTAAAGCCCTTATTCGTATAATAg                                                                      <  1:828412/180‑1 (MQ=255)                                                                                                                                                                      ttaccATTATCTCCATATAAGCGGTCATCACCATCATAACCATAAATCACATCATCACCATCGTGACCATGGAAAACATCATTAAATCTAGAACCATAAAAATGATCCCGTAATTTACTGCCGATAATTTCCTCTACTGAGTTTAATTCATCATGAGCTTTAAAGCCCTTATTCGTATAATAGTAATCTAATTTATAGTTACGATATTCAAGTAGATCGGTAACCTTCCCTCGTTTAGTTTCTTGCTTTGa  >  1:694552/4‑251 (MQ=255)                                                                                                                                                                                 |                                                                                                                                                                                                                                                TCATTGCCACTTCCACCTCTTAGAATATCGGAACCATTGCCTCCCTCTAAGTGGTCGTCGCCTTCTCCACCGTCAAGATAGTTGTTGCCATATTCACCAAAGAGCCTATCGTTACCGGCACCACCATAGAGCTTATCATTACCTTGGCCGCCATGAATTTCGTCATTCCCATTATCGCCATACAAACGATCATCGCCATCATAACCATAAATCAAATCATCGCCATCGTGACCATGGAAAACATCATTAAATTTAGAACCATAAAATTTATCACGTAGTGTGCTGCCGATAATTTCCTCTACTGAGTTTAATTCATCATGAGCTTTAAAGCCCTTATTCGTATAATAGTAATCTAATTTATAGTTACGATATTCAAGTAGATCGGTAACCTTCCCTCGTTTAGTTTCTTGCTTTGA  >  1/1979677‑1980092

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑