Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,979,727	G→A	H800H (CAC→CAT)	unknown ←	RTX toxins determinant A and related Ca2+‑binding proteins

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,979,727	0	G	A	100.0%	143.5 / NA	50	H800H (CAC→CAT)	unknown	RTX toxins determinant A and related Ca2+‑binding proteins
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (24/26);  total (24/26)

TCAAATGACACATCTTTGAGATTGATATTTGCTAATGATAATTTATCTTTATCACCGCTATGTTCCGTAATAGTGTGGTGCCCATATTCTTTACGGTAAACATAAATATCATTTCCTTCTCCACCGGCAAGTTGGTCATCGCCTTCTCCACCGTCAAGTAAATCATCTCCTTGGTTTCCAAACAACTTATCATTGCCACTTCCACCTCTTAGAATATCGGAACCATTGCCTCCCTCTAAGTGGTCGTCGCCTTCTCCACCGTCAAGATAGTTGTTGCCATATTCACCAAAGAGCCTATCGTTACCGGCACCACCATAGAGCTTATCATTACCTTGGCCGCCATGAATTTCGTCATTCCCATTATCGCCATACAAACGATCATCGCCATCATAACCATAAATCAAATCATCGCCATCGTGACCATGGAAAACATCATTAAATTTAGAACCATAAAATTTATCACGTAGTGTGC  >  1/1979488‑1979959                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                         tCAAATGACACCTCTTTGAGGTTGATATTTGCTAATGATAATTTATCTTTATCACCGCTATGTTCCGTAATAGTGTGGTGGCCATATTCCTTACGGTAAACATAAATATCATTTCCTTCTCCACCGGCAAGTTGATCATCACCTTTTCCGCCATCAAGCAAGTCATCTCCTTGGTTTCCAAATAATTTATCATTGCCACTTCCGCCTCTTAGAATATCGGAACCATTCCCCCCTTCTAAATGGTCGTCGcc                                                                                                                                                                                                                               <  1:825617/251‑1 (MQ=255)        acacCTCTTTGAGGTTGATATTTGCTAATGATAATTTATCTTTATCACCGCTATGTTCCGTAATAGTGTGGTGGCCATATTCCTTACGGTAAACATAAATATCATTTCCTTCTCCACCGGCAAGTTGATCATCACCTTTTCCGCCATCGAGCAAGTCATCTCCTTGGTTTCCAAATAATTTATCATTGCCACTTCCGCCTCTTAGAATATCGGAACCATTCCCCCCTTCTAAATGGTCGTCGCCTTCACCa                                                                                                                                                                                                                        <  1:19770/251‑1 (MQ=255)        acacCTCTTTGAGGTTGATATTTGCTAATGATAATTTATCTTTATCACCGCTATGTTCCGTAATAGTGTGGTGGCCATATTCCTTACGGTAAACATAAATATCATTTCCTTCTCCACCGGCAAGTTGATCATCACCTTTTCCGCCATCAAGCAAGTCATCTCCTTGGTTTCCAAATAATTTATCATTGCCACTTCCGCCTCTTAGAATATCGGAACCATTCCCCCCTTCTAAATGGTCGTCGCCTtcacc                                                                                                                                                                                                                         <  1:47213/250‑4 (MQ=255)            ctCTTTGAGGTTGATATTTGCTAATGATAATTTATCTTTATCACCGCTATGTTCCGTAATAGTGTGGTGGCCATATTCCTTACGGTAAACATAAATATCATTTCCTTCTCCACCGGCAAGTTGATCATCACCTTTTCCGCCATCAAGCAAGTCATCTCCTTGGTTTCCAAATAATTTATCATTGCCACTTCCGCCTCTTAGAATATCGGAACCATTCCCCCCTTCTAAATGGTCGTCGCCTtcacc                                                                                                                                                                                                                         <  1:413163/245‑4 (MQ=255)              cTTTGAGGTTGATATTTGCTAATGATAATTTATCTTTATCACCGCTATGTTCCGTAATAGTGTGGTGGCCATATTCCTTACGGTAAACATAAATATCATTTCCTTCTCCACCGGCAAGTTGATCATCACCTTTTCCGCCATCAAGCAAGTCATCTCCTTGGTTTCCAAATAATTTATCATTGCCACTTCCGCCTCTTAGAATATCGGAACCATTCCCCCCTTCTAAATGGTCGTCGCCTTCACCACCGt                                                                                                                                                                                                                    >  1:676970/1‑249 (MQ=255)              cTTTGAGGTTGATATTTGCTAATGATAATTTATCTTTATCACCGCTATGTTCCGTAATAGTGTGGTGGCCATATTCCTTACGGTAAACATAAATATCATTTCCTTCTCCACCGGCAAGTTGATCATCACCTTTTCCGCCATCAAGCAAGTCATCTCCTTGGTTTCCAAATAATTTATCATTGCCACTTCCGCCTCTTAGAATATCGGAACCATTCCCCCCTTCTAAATGGTCGTCGCCTTCACCACCGt                                                                                                                                                                                                                    >  1:24648/1‑249 (MQ=255)                ttGAGGTTGATATTTGCTAATGATAATTTATCTTTATCACCGCTATGTTCCGTAATAGTGTGGTGGCCATATTCCTTACGGTAAACATAAATATCATTTCCTTCTCCACCGGCAAGTTGATCATCACCTTTTCCGCCATCAAGCAAGTCATCTCCTTGGTTTCCAAATAATTTATCATTGCCACTTCCGCCTCTTAGAATATCGGAACCATTCCCCCCTTCTAAATGGTCGTCGCCTTCACCACCGTctag                                                                                                                                                                                                                <  1:92271/251‑4 (MQ=255)                   aggttGATATTTGCTAATGATAATTTATCTTTATCACCGCTATGTTCCGTAATAGTGTGGTGGCCATATTCCTTACGGTAAACATAAATATCATTTCCTTCTCCACCGGCAAGTTGATCATCACCTTTTTCGCCATCAAGCAAGTCATCTCCTTGGTTTCCAAATAATTTATCATTGCCACTTCCGCCTCTTAGAATATCGGAACCATTCCCCCCTTCTAAATGGTCGTCGCCTTCACCACCGTCTAGAt                                                                                                                                                                                                              <  1:178795/247‑1 (MQ=255)                   aggttGATATTTGCTAATGATAATTTATCTTTATCACCGCTATGTTCCGTAATAGTGTGGTGGCCATATTCCTTACGGTAAACATAAATATCATTTCCTTCTCCACCGGCAAGTTGATCATCACCTTTTCCGCCATCAAGCAAGTCATCTCCTTGGTTTCCAAATAATTTATCATTGCCACTTCCGCCTCTTAGAATATCGGAACCATTCCCCCCTTCTAAATGGTCGTCGCCTTCACCACCGTCTAGAt                                                                                                                                                                                                              <  1:461324/247‑1 (MQ=255)                   aggttGATATTTGCTAATGATAATTTATCTTTATCACCGCTATGTTCCGTAATAGTGTGGTGGCCATATTCCTTACGGTAAACATAAATATCATTTCCTTCTCCACCGGCAAGTTGATCATCACCTTTTCCGCCATCAAGCAAGTCATCTCCTTGGTTTCCAAATAATTTATCATTGCCACTTCCGCCTCTTAGAATATCGGAACCATTCCCCCCTTCTAAATGGTCGTCGCCTTCACCACCGTCTAGAt                                                                                                                                                                                                              <  1:604227/247‑1 (MQ=255)                   aggttGATATTTGCTAATGATAATTTATCTTTATCACCGCTATGTTCCGTAATAGTGTGGTGGCCATATTCCTTACGGTAAACATAAATATCATTTCCTTCTCCACCGGCAAGTTGATCATCACCTTTTCCGCCATCAAGCAAGTCATCTCCTTGGTTTCCAAATAATTTATCATTGCCACTTCCGCCTCTTAGAATATCGGAACCATTCCCCCCTTCTAAATGGTCGTCGCCTTCACCACCGTCTAGAt                                                                                                                                                                                                              <  1:793025/247‑1 (MQ=255)                     gttGATATTTGCTAATGATAATTTATCTTTATCACCGCTATGTTCCGTAATAGTGTGGTGGCCATATTCCTTACGGTAAACATAAATATCATTTCCTTCTCCACCGGCAAGTTGATCATCACCTTTTCCGCCATCAAGCAAGTCATCTCCTTGGTTTCCAAATAATTTATCATTGCCACTTCCGCCTCTTAGAATATCGGAACCATTCCCCCCTTCTAAATGGTCGTCGCCTTCACCACCGTCTAGAt                                                                                                                                                                                                              >  1:672489/2‑248 (MQ=255)                       tGATATTTGCTAATGATAATTTATCTTTATCACCGCTATGTTCCGTAATAGTGTGGTGGCCATATTCCTTACGGTAAACATAAATATCATTTCCTTCTCCACCGGCAAGTTGATCATCACCTTTTCCGCCATCAAGCAAGTCATCTCCTTGGTTTCCAAATAATTTATCATTGCCACTTCCGCCTCTTAGAATATCGGAACCATTCCCCCCTTCTAAATGGTCGTCGCCTTCACCACCGTCTAGATAgttg                                                                                                                                                                                                         <  1:236144/251‑1 (MQ=255)                                cTAATGATAATTTATCTTTATCACCGCTATGTTCCGTAATAGTGTGGTGGCCATATTCCTTACGGTAAACATAAATATCATTTCCTTCTCCACCGGCAAGTTGATCATCACCTTTTCCGCCATCAAGCAAGTCATCTCCTTGGTTTCCAAATAATTTATCATTGCCACTTCCGCCTCTTAGAATATCGGAACCATTCCCCCCTTCTAAATGGTCGTCGCCTTCACCACCGTCTAGATAGTTGTTACCatat                                                                                                                                                                                                <  1:600827/251‑1 (MQ=255)                                       tAATTTATCTTTATCACCGCTATGTTCCGTAATAGTGTGGTGGCCATATTCCTTACGGTAAACATAAATATCATTTCCTTCTCCACCGGCAAGTTGATCATCACCTTTTCCGCCATCAAGCAAGTCATCTCCTTGGTTTCCAAATAATTTATCATTGCCACTTCCGCCTCTTAGAATATCGGAACCATTCCCCCCTTCTAAATGGTCGTCGCCTTCACCACCGTCTAGATAGTTGTTACCATATTCACCaa                                                                                                                                                                                         <  1:660471/251‑1 (MQ=255)                                         aTTTATCTTTATCACCGCTATGTTCCGTAATAGTGTGGTGGCCATATTCCTTACGGTAAACATAAATATCATTTCCTTCTCCACCGGCAAGTTGATCATCACCTTTTCCGCCATCAAGCAAGTCATCTCCTTGGTTTCCAAATAATTTATCATTGCCACTTCCGCCTCTTAGAATATCGGAACCATTCCCCCCTTCTAAATGGTCGTCGCCTTCACCACCGTCTAGATAGTTGTTACCATATTCACCAAag                                                                                                                                                                                       <  1:549865/251‑1 (MQ=255)                                                    tcACCGCTATGTTCCGTAATAGTGTGGTGGCCATATTCCTTACGGTAAACATAAATATCATTTCCTTCTCCACCGGCAAGTTGATCATCACCTTTTCCGCCATCAAGCAAGTCATCTCCTTGGTTTCCAAATAATTTATCATTGCCACTTCCGCCTCTTAGAATATCGGAACCATTCCCCCCTTCTAAATgg                                                                                                                                                                                                                                       <  1:395947/192‑1 (MQ=255)                                                       ccGCTATGTTCCGTAATAGTGTGGTGGCCATATTCCTTACGGTAAACATAAATATCATTTCCTTCTCCACCGGCAAGTTGATCATCACCTTTTCCGCCATCAAGCAAGTCATCTCCTTGGTTTCCAAATAATTTATCATTGCCACTTCCGCCTCTTAGAATATCGGAACCATTCCCCCCTTCTAAATGGTCGTCGCCTTcaccac                                                                                                                                                                                                                       >  1:895642/1‑205 (MQ=255)                                                       ccGCTATGTTCCGTAATAGTGTGGTGGCCATATTCCTTACGGTAAACATAAATATCATTTCCTTCTCCACCGGCAAGTTGATCATCACCTTTTCCGCCATCAAGCAAGTCATCTCCTTGGTTTCCAAATAATTTATCATTGCCACTTCCGCCTCTTAGAATATCGGAACCATTCCCCCCTTCTAAATGGTCGTCGCCTTCACCACCGTCTAGATAGTTGTTACCATATTCACCAAAGAGCCtgtcattgc                                                                                                                                                                          >  1:385254/1‑242 (MQ=255)                                                            aTGTTCCGTAATAGTGTGGTGGCCATATTCCTTACGGTAAACATAAATATCATTTCCTTCTCCACCGGCAAGTTGATCATCACCTTTTCCGCCATCAAGCAAGTCATCTCCTTGGTTTCCAAATAATTTATCATTGCCACTTCCGCCTCTTAGAATATCGGAACCATTCCCCCCTTCTAAATGGTCGTCGCCTTCACCACCGTCTAGATAgttg                                                                                                                                                                                                         <  1:419764/214‑1 (MQ=255)                                                              gTTCCGTAATAGTGTGGTGGCCATATTCCTTACGGTAAACATAAATATCATTTCCTTCTCCACCGGCAAGTTGATCATCACCTTTTCCGCCATCAAGCAAGTCATCTCCTTGGTTTCCAAATAATTTATCATTGCCACTTCCGCCTCTTAGAATATCGGAACCATTCCCCCCTTCTAAATGGTCGTCGCCTTCACCACCGTCTAGATAGTTGTTACCATATTCACCAAAGAGCCTGTCATTGCCGGcgcca                                                                                                                                                                  >  1:527365/1‑251 (MQ=255)                                                               ttCCGTAATAGTGTGGTGGCCATATTCCTTACGGTAAACATAAATATCATTTCCTTCTCCACCGGCAAGTTGATCATCACCTTTTCCGCCATCAAGCAAGTCATCTCCTTGGTTTCCAAATAATTTATCATTGCCACTTCCGCCTCTTAGAATATCGGAACCATTCCCCCCTTCTAAATGGTCGTCGCCTTcaccac                                                                                                                                                                                                                       >  1:654430/1‑197 (MQ=255)                                                                 ccGTAATAGTGTGGTGGCCATATTCCTTACGGTAAACATAAATATCATTTCCTTCTCCACCGGCAAGTTGATCATCACCTTTTCCGCCATCAAGCAAGTCATCTCCTTGGTTTCCAAATAATTTATCATTGCCACTTCCGCCTCTTAGAATATCGGAACCATTCCCCCCTTCTAAATGGTCGTCGCCTTCACCACCGTCTAGATAGTTGTTACCat                                                                                                                                                                                                  >  1:843668/1‑216 (MQ=255)                                                                 ccGTAATAGTGTGGTGGCCATATTCCTTACGGTAAACATAAATATCATTTCCTTCTCCACCGGCAAGTTGATCATCACCTTTTCCGCCATCAAGCAAGTCATCTCCTTGGTTTCCAAATAATTTATCATTGCCACTTCCGCCTCTTAGAATATCGGAACCATTCCCCCCTTCTAAATGGTCGTCGCCTTCACCACCGTCTAGATAGTTGTTACCATATTCACCAAAGAGCCTGTCATTGCCGGCGccacca                                                                                                                                                               >  1:154513/1‑251 (MQ=255)                                                                 ccGTAATAGTGTGGTGGCCATATTCCTTACGGTAAACATAAATATCATTTCCTTCTCCACCGGCAAGTTGATCATCACCTTTTCCGCCATCAAGCAAGTCATCTCCTTGGTTTCCAAATAATTTATCATTGCCACTTCCGCCTCTTAGAATATCGGAACCATTCCCCCCTTCTAAATGGTCGTCGCCTTCACCACCGTCTAGATAGTTGTTACCATATTCACCAAAGAGCCTGTCATTGCCGGCGccacca                                                                                                                                                               >  1:540493/1‑251 (MQ=255)                                                                 ccGTAATAGTGTGGTGGCCATATTCCTTACGGTAAACATAAATATCATTTCCTTCTCCACCGGCAAGTTGATCATCACCTTTTCCGCCATCAAGCAAGTCATCTCCTTGGTTTCCAAATAATTTATCATTGCCACTTCCGCCTCTTAGAATATCGGAACCATTCCCCCCTTCTAAATGGTCGTCGCCTTCACCACCGTCTAGATAGTTGTTACCATATTCACCAAAGAGCCTGTCATTGCCGGCGccacca                                                                                                                                                               >  1:667289/1‑251 (MQ=255)                                                                      aTAGTGTGGTGGCCATATTCCTTACGGTAAACATAAATATCATTTCCTTCTCCACCGGCAAGTTGATCATCACCTTTTCCGCCATCAAGCAAGTCATCTCCTTGGTTTCCAAATAATTTATCATTGCCACTTCCGCCTCTTAGAATATCGGAACCATTCCCCCCTTCTAAATGGTCGTCGCCTTCACCACCGTCTAGATAGTTGTTACCATATTCACCAAagag                                                                                                                                                                                     <  1:113284/224‑1 (MQ=255)                                                                      aTAGTGTGGTGGCCATATTCCTTACGGTAAACATAAATATCATTTCCTTCTCCACCGGCAAGTTGATCATCACCTTTTCCGCCATCAAGCAAGTCATCTCCTTGGTTTCCAAATAATTTATCATTGCCACTTCCGCCTCTTAGAATATCGGAACCATTCCCCCCTTCTAAATGGTCGTCGCCTTCACCACCGTCTAGATAGTTGTTACCATATTCACCAAAGAGCCtgtcattg                                                                                                                                                                           <  1:439535/234‑8 (MQ=255)                                                                      aTAGTGTGGTGGCCATATTCCTTACGGTAAACATAAATATCATTTCCTTCTCCACCGGCAAGTTGATCATCACCTTTTCCGCCATCAAGCAAGTCATCTCCTTGGTTTCCAAATAATTTATCATTGCCACTTCCGCCTCTTAGAATATCGGAACCATTCCCCCCTTCTAAATGGTCGTCGCCTTCACCACCGTCTAGATAGTTGTTACCATATTCACCAAAGAGCCTGTCATTGCCGGCGCCACCATaaag                                                                                                                                                          <  1:86129/251‑4 (MQ=255)                                                                      aTAGTGTGGTGGCCATATTCCTTACGGTAAACATAAATATCATTTCCTTCTCCACCGGCAAGTTGATCATCACCTTTTCCGCCATCAAGCAAGTCATCTCCTTGGTTTCCAAATAATTTATCATTGCCACTTCCGCCTCTTAGAATATCGGAACCATTCCCCCCTTCTAAATGGTCGTCGCCTTCACCACCGTCTAGATAGTTGTTACCATATTCACCAAAGAGCCTGTCATTGCCGGCGCCACCATaaag                                                                                                                                                          >  1:319844/1‑248 (MQ=255)                                                                       tAGTGTGGTGGCCATATTCCTTACGGTAAACATAAATATCATTTCCTTCTCCACCGGCAAGTTGATCATCACCTTTTCCGCCATCAAGCAAGTCATCTCCTTGGTTTCCAAATAATTTATCATTGCCACTTCCGCCTCTTAGAATATCGGAACCATTCCCCCCTTCTAAATGGTCGTCGCCTTCACCACCGTCTAGATAGTTGTTACCATATTCACCAAAGAGCCTGTCATTGCCGGCGCCACCATaaagt                                                                                                                                                         <  1:482596/251‑5 (MQ=255)                                                                        aGTGTGGTGGCCATATTCCTTACGGTAAACATAAATATCATTTCCTTCTCCACCGGCAAGTTGATCATCACCTTTTCCGCCATCAAGCAAGTCATCTCCTTGGTTTCCAAATAATTTATCATTGCCACTTCCGCCTCTTAGAATATCGGAACCATTCCCCCCTTCTAAATGGTCGTCGCCTTCACCACCGTCTAGATAGTTGTTACCATATTCACCAAAGAGCCTGTCATTGCCGGCGCCACCATaaagtt                                                                                                                                                        >  1:289420/1‑246 (MQ=255)                                                                         gtgtGGTGGCCATATTCCTTACGGTAAACATAAATATCATTTCCTTCTCCACCGGCAAGTTGATCATCACCTTTTCCGCCATCAAGCAAGTCATCTCCTTGGTTTCCAAATAATTTATCATTGCCACTTCCGCCTCTTAGAATATCGGAACCATTCCCCCCTTCTAAATGGTCGTCGCCTTCACCACCGTCTAGATAGTTGTTACCATATTCACCAAAGAGCCTGTCATTGCCGGCGCCACCATaaagttt                                                                                                                                                       >  1:79381/1‑245 (MQ=255)                                                                           gtggtgGCCATATTCCTTACGGTAAACATAAATATCATTTCCTTCTCCACCGGCAAGTTGATCATCACCTTTTCCGCCATCAAGCAAGTCATCTCCTTGGTTTCCAAATAATTTATCATTGCCACTTCCGCCTCTTAGAATATCGGAACCATTCCCCCCTTCTAAATGGTCGTCGCCTTCACCACCGTCTAGATAGTTGTTACCata                                                                                                                                                                                                 >  1:163873/1‑207 (MQ=255)                                                                             ggtgGCCATATTCCTTACGGTAAACATAAATATCATTTCCTTCTCCACCGGCAAGTTGATCATCACCTTTTCCGCCATCAAGCAAGTCATCTCCTTGGTTTCCAAATAATTTATCATTGCCACTTCCGCCTCTTAGAATATCGGAACCATTCCCCCCTTCTAAATGGTCGTCGCCTTCACCACCGTCTAGATAGTTGTTACCATATTCACCAAAGAGCCTGTCATTGCCGGCGCCACCATaaagtttgtcg                                                                                                                                                   >  1:611300/1‑241 (MQ=255)                                                                                ggccATATTCCTTACGGTAAACATAAATATCATTTCCTTCTCCACCGGCAAGTTGATCATCACCTTTTCCGCCATCAAGCAAGTCATCTCCTTGGTTTCCAAATAATTTATCATTGCCACTTCCGCCTCTTAGAATATCGGAACCATTCCCCCCTTCTAAATGGTCGTCGCCTTCACCACCGTCTAGATAGTTGTTACCATATTCACCAAAGAGCCTGTCATTGCCGGCGCCACCATaaagtttgtcgttg                                                                                                                                                >  1:340776/3‑238 (MQ=255)                                                                                 gccATATTCCTTACGGTAAACATAAATATCATTTCCTTCTCCACCGGCAAGTTGATCATCACCTTTTCCGCCATCAAGCAAGTCATCTCCTTGGTTTCCAAATAATTTATCATTGCCACTTCCGCCTCTTAGAATATCGGAACCATTCCCCCCTTCTAAATGGTCGTCGCCTTCACCACCGTCTAGAt                                                                                                                                                                                                              <  1:135531/187‑1 (MQ=255)                                                                                 gccATATTCCTTACGGTAAACATAAATATCATTTCCTTCTCCACCGGCAAGTTGATCATCACCTTTTCCGCCATCAAGCAAGTCATCTCCTTGGTTTCCAAATAATTTATCATTGCCACTTCCGCCTCTTAGAATATCGGAACCATTCCCCCCTTCTAAATGGTCGTCGCCTTCACCACCGTCTAGATAGTTGTTACCATATTCACCAAAGAGCCTGTCATTGCCGGCGCCACCATaaagtttgtcgttg                                                                                                                                                >  1:226947/2‑237 (MQ=255)                                                                                 gccATATTCCTTACGGTAAACATAAATATCATTTCCTTCTCCACCGGCAAGTTGATCATCACCTTTTCCGCCATCAAGCAAGTCATCTCCTTGGTTTCCAAATAATTTATCATTGCCACTTCCGCCTCTTAGAATATCGGAACCATTCCCCCCTTCTAAATGGTCGTCGCCTTCACCACCGTCTAGATAGTTGTTACCATATTCACCAAAGAGCCTGTCATTGCCGGCGCCACCATaaagtttgtcgttg                                                                                                                                                >  1:596773/2‑237 (MQ=255)                                                                                      atTCCTTACGGTAAACATAAATATCATTTCCTTCTCCACCGGCAAGTTGATCATCACCTTTTCCGCCATCAAGCAAGTCATCTCCTTGGTTTCCAAATAATTTATCTTTGCCACTTCCGCCTCTTAGAATATCGGAACCATTCCCCCCTTCTAAATGGTCGTCGCCTTCACCACCGTCTAGATAGTTGTTACCATATTCACCaa                                                                                                                                                                                         >  1:760071/1‑204 (MQ=255)                                                                                         ccttACGGTAAACATAAATATCATTTCCTTCTCCACCGGCAAGTTGATCATCACCTTTTCCGCCATCAAGCAAGTCATCTCCTTGGTTTCCAAATAATTTATCATTGCCACTTCCGCCTCTTAGAATATCGGAACCATTCCCCCCTTCTAAATgg                                                                                                                                                                                                                                       >  1:890113/3‑155 (MQ=255)                                                                                                    aCATAAATATCATTTCCTTCTCCACCGGCAAGTTGATCATCACCTTTTCCGCCATCAAGCAAGTCATCTCCTTGGTTTCCAAATAATTTATCATTGCCACTTCCGCCTCTTAGAATATCGGAACCATTCCCCCCTTCTAAATGGTCGTCGCCTTCACCACCGTCTAGATAGTTGTTACCATATTCACCAAAGAGCCtgtcattgcc                                                                                                                                                                         >  1:669250/1‑197 (MQ=255)                                                                                                                                                                                              tCATTGCCACTTCCGCCTCTTAGAATATCGGAACCATTCCCCCCTTCTAAATGGTCGTCGCCTTCACCACCGTCTAGATAGTTGTTACCATATTCACCAAAGAGCCTGTCATTGCCGGCGCCACCATAAAGTTTGTCGTTGCCTTTTCCTCCATGGATCTCATCATTACCATTATCTCCATATAAGCGGTCATCACCATCATAACCATAAATCACATCATCACCATCGTGACCATGGAAAACATCATTaaa                                  <  1:870761/251‑1 (MQ=255)                                                                                                                                                                                                              ctctTAGAATATCGGAACCATTCCCCCCTTCTAAATGGTCGTCGCCTTCACCACCGTCTAGATAGTTGTTACCATATTCACCAAAGAGCCTGTCATTGCCGGCGCCACCATAAAGTTTGTCGTTGCCTTTTCCTCCATGGATCTCATCATTACCATTATCTCCATATAAGCGGTCATCACCATCATAACCATAAATCACATCATCACCATCGTGACCATGGAAAACATCATTAAATCTAGAACCATaaaaa                  <  1:885681/251‑2 (MQ=255)                                                                                                                                                                                                               tctTAGAATATCGGAACCATTCCCCCCTTCTAAATGGTCGTCGCCTTCACCACCGTCTAGATAGTTGTTACCATATTCACCAAAGAGCCTGTCATTGCCGGCGCCACCATAAAGTTTGTCGTTGCCTTTTCCTCCATGGATCTCATCATTACCATTATCTCCATATAAGCGGTCATCACCATCATAACCATAAATCACATCATCACCATCGTGACCATGGAAAACATCATTAAAt                                 <  1:471351/235‑1 (MQ=255)                                                                                                                                                                                                               tctTAGAATATCGGAACCATTCCCCCCTTCTAAATGGTCGTCGCCTTCACCACCGTCTAGATAGTTGTTACCATATTCACCAAAGAGCCTGTCATTGCCGGCGCCACCATAAAGTTTGTCGTTGCCTTTTCCTCCATGGATCTCATCATTACCATTATCTCCATATAAGCGGTCATCACCATCATAACCATAAATCACATCATCACCATCGTGACCATGGAAAACATCATTAAATCTAGAACCATaaaaat                 >  1:273575/1‑249 (MQ=255)                                                                                                                                                                                                                ctTAGAATATCGGAACCATTCCCCCCTTCTAAATGGTCGTCGCCTTCACCACCGTCTAGATAGTTGTTACCATATTCACCAAAGAGCCTGTCATTGCCGGCGCCGCCATAAAGTTTGTCGTTGCCTTTTCCTCCATGGATCTCATCATTACCATTATCTCCATATAAGCGGTCATCACCATCATAACCATAAATCACATCATCACCATCGTGACCATGGAAAACATCATTAAATCTAGAACCATaaaaatg                <  1:210238/251‑4 (MQ=255)                                                                                                                                                                                                                              aCCATTCCCCCCTTCTAAATGGTCGTCGCCTTCACCACCGTCTAGATAGTTGTTACCATATTCACCAAAGAGCCTGTCATTGCCGGCGCCACCATAAAGTTTGTCGTTGCCTTTTCCTCCATGGATCTCATCATTACCATTATCTCCATATAAGCGGTCATCACCATCATAACCATAAATCACATCATCACCATCGTGACCATGGAAAACATCATTAAATCTAGAACCATaaaaatgatcccgtaatttac  <  1:693251/251‑18 (MQ=255)                                                                                                                                                                                                                              aCCATTCCCCCCTTCTAAATGGTCGTCGCCTTCACCACCGTCTAGATAGTTGTTACCATATTCACCAAAGAGCCTGTCATTGCCGGCGCCACCATAAAGTTTGTCGTTGCCTTTTCCTCCATGGATCTCATCATTACCATTATCTCCATATAAGCGGTCATCACCATCATAACCATAAATCACATCATCACCATCGTGACCATGGAAAACATCATTAAATCTAGAACCATaaaaatgatcccgtaatttac  <  1:344235/251‑18 (MQ=255)                                                                                                                                                                                                                                    ccccccttctAAATGGTCGTCGCCTTCACCACCGTCTAGATAGTTGTTACCATATTCACCAAAGAGCCTGTCATTGCCGGCGCCACCATAAAGTTTGTCGTTGCCTTTTCCTCCATGGATCTCATCATTACCATTATCTCCATATAAGCGGTCATCACCATCATAACCATAAATCACATCATCACCATCGTGACCATGGAAAACATCATTAAATCTAGAACCATaa                     >  1:500519/8‑226 (MQ=255)                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                         TCAAATGACACATCTTTGAGATTGATATTTGCTAATGATAATTTATCTTTATCACCGCTATGTTCCGTAATAGTGTGGTGCCCATATTCTTTACGGTAAACATAAATATCATTTCCTTCTCCACCGGCAAGTTGGTCATCGCCTTCTCCACCGTCAAGTAAATCATCTCCTTGGTTTCCAAACAACTTATCATTGCCACTTCCACCTCTTAGAATATCGGAACCATTGCCTCCCTCTAAGTGGTCGTCGCCTTCTCCACCGTCAAGATAGTTGTTGCCATATTCACCAAAGAGCCTATCGTTACCGGCACCACCATAGAGCTTATCATTACCTTGGCCGCCATGAATTTCGTCATTCCCATTATCGCCATACAAACGATCATCGCCATCATAACCATAAATCAAATCATCGCCATCGTGACCATGGAAAACATCATTAAATTTAGAACCATAAAATTTATCACGTAGTGTGC  >  1/1979488‑1979959

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑