Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,979,628	G→A	G833G (GGC→GGT)	unknown ←	RTX toxins determinant A and related Ca2+‑binding proteins

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,979,628	0	G	A	100.0%	177.1 / NA	64	G833G (GGC→GGT)	unknown	RTX toxins determinant A and related Ca2+‑binding proteins
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (34/30);  total (34/30)

CATCTAATCCTGCCGATGAATTAGGTTTACTAAACCATCCTTTAAATGTTACTGCTGTTCTATTATTTGTTTTCAATAGTAGATCATTGCCGTTACGCTCAAATGACACATCTTTGAGATTGATATTTGCTAATGATAATTTATCTTTATCACCGCTATGTTCCGTAATAGTGTGGTGCCCATATTCTTTACGGTAAACATAAATATCATTTCCTTCTCCACCGGCAAGTTGGTCATCGCCTTCTCCACCGTCAAGTAAATCATCTCCTTGGTTTCCAAACAACTTATCATTGCCACTTCCACCTCTTAGAATATCGGAACCATTGCCTCCCTCTAAGTGGTCGTCGCCTTCTCCACCGTCAAGATAGTTGTTGCCATATTCACCAAAGAGCCTATCGTTACCGGCACCACCATAGAGCTTATCATTA  >  1/1979390‑1979817                                                                                                                                                                                                                                               |                                                                                                                                                                                              catttaaccccgCCGACGAATTAGGTTTATTAAACCATCCTTTAAATGTTACTGCTGTTCTATTATTTGTTTTCAATAGTAGATCATTGCCGTTTCGTTCAAATGACACCTCTTTGAGGTTGATATTTGCTAATGATAATTTATCTTTATCACCGCTATGTTCCGTAATAGTGTGGTGGCCATATTCCTTACGGTAAACAGAAATATCATTTCCTTCTCCACCGGCAAGTTGATCATCACCt                                                                                                                                                                                            <  1:48027/231‑1 (MQ=255)                 cgAATTAGGTTTATTAAACCATCCTTTAAATGTTACTGCTGTTCTATTATTTGTTTTCAATAGTAGATCATTGCCGTTACGTTCAAATGACACCTCTTTGAGGTTGATATTTGCTAATGATAATTTATCTTTATCACCGCTATGTTCCGTAATAGTGTGGTGGCCATATTCCTTACGGTAAACATAAATATCATTTCCTTCTCCACCGGCAAGTTGATCATCACCTTTTCCGCCATCAAGCAAGTCAtctc                                                                                                                                                                   <  1:645673/250‑1 (MQ=255)                         gTTTATTAAACCATCCTTTAAATGTTACTGCTGTTCTATTATTTGTTTTCAATAGTAGATCATTGCCGTTACGTTCAAATGACACCTCTTTGAGGTTGATATTTGCTAATGATAATTTATCTTTATCACCGCTATGTTCCGTAATAGTGTGGTGGCCATATTCCTTACGGTAAACATAAATATCATTTCCTTCTCCACCGGCAAGTTGATCATCACCTTTTCCGCCATCAAGCAAGTCATCTCCTTGGttt                                                                                                                                                           >  1:601527/1‑251 (MQ=255)                                               tGTTACTGCTGTTCTATTATTTGTTTTCAATAGTAGATCATTGCCGTTACGTTCAAATGACACCTCTTTGAGGTTGATATTTGCTAATGATAATTTATCTTTATCACCGCTATGTTCCGTAATAGTGTGGTGGCCATATTCCTTACGGTAAACATAAATATCATTTCCTTCTCCACCGGCAAGTTGATCATCACCTTTTCCGCCATCAAGCAAGTCATCTCCTTGGTTTCCAAATAATTTATCATTGCCa                                                                                                                                      >  1:880111/1‑250 (MQ=255)                                                  tACTGCTGTTCTATTATTTGTTTTCAATAGTAGATCATTGCCGTTACGTTCAAATGACACCTCTTTGAGGTTGATATTTGCTAATGATAATTTATCTTTATCACCGCTATGTTCCGTAATAGTGTGGTGGCCATATTCCTTACGGTAAACATAAATATCATTTCCTTCTCCACCGGCAAGTTGATCATCACCTTTTCCGCCATCAAGCAAGTCATCTCCTTGGTTTCCAAATAATTTATCATTGCCACTTc                                                                                                                                  <  1:573313/251‑1 (MQ=255)                                                         gTTCTATTATTTGTTTTCAATAGTAGATCATTGCCGTTACGTTCAAATGACACCTCTTTGAGGTTGATATTTGCTAATGATAATTTATCTTTATCACCGCTATGTTCCGTAATAGTGTGGTGGCCATATTCCTTACGGTAAACATAAATATCATTTCCTTCTCCACCGGCAAGTTGATCATCACCTTTTCCGCCATCAAGCAAGTCATCTCCTTGGTTTCCAAATAATTTATCATTGCCACtt                                                                                                                                   <  1:542347/243‑1 (MQ=255)                                                         gTTCTATTATTTGTTTTCAATAGTAGATCATTGCCGTTACGTTCAAATGACACCTCTTTGAGGTTGATATTTGCTAATGATAATTTATCTTTATCACCGCTATGTTCCGTAATAGTGTGGTGGCCATATTCCTTACGGTAAACATAAATATCATTTCCTTCTCCACCGGCAAGTTGATCATCACCTTTTCCGCCATCAAGCAAGTCATCTCCTTGGTTTCCAAATAATTTATCATTGCCACTTCCGCct                                                                                                                             >  1:75758/1‑249 (MQ=255)                                                         gTTCTATTATTTGTTTTCAATAGTAGATCATTGCCGTTACGTTCAAATGACACCTCTTTGAGGTTGATATTTGCTAATGATAATTTATCTTTATCACCGCTATGTTCCGTAATAGTGTGGTGGCCATATTCCTTACGGTAAACATAAATATCATTTCCTTCTCCACCGGCAAGTTGATCATCACCTTTTCCGCCATCAAGCAAGTCATCTCCTTGGTTTCCAAATAATTTATCATTGCCACTTCCGCct                                                                                                                             >  1:438786/1‑249 (MQ=255)                                                               ttattTGTTTTCAATAGTAGATCATTGCCGTTACGTTCAAATGACACCTCTTTGAGGTTGATATTTGCTAATGATAATTTATCTTTATCACCGCTATGTTCCGTAATAGTGTGGTGGCCATATTCCTTACGGTAAACATAAATATCATTTCCTTCTCCACCGGCAAGTTGATCATCACCTTTTCCGCCATCAAGCAAGTCATCTCCTTGGTTTCCAAATAATTTATCATTGCCACTTCCGCCTCTTAGAat                                                                                                                     <  1:310299/251‑1 (MQ=255)                                                                            aTAGTAGATCATTGCCGTTACGTTCAAATGACACCTCTTTGAGGTTGATATTTGCTAATGATAATTTATCTTTATCACCGCTATGTTCCGTAATAGTGTGGTGGCCATATTCCTTACGGTAAACATAAATATCATTTCCTTCTCCACCGGCAAGTTGATCATCACCTTTTCCGCCATCAAGCAAGTCATCTCCTTgg                                                                                                                                                              >  1:78356/1‑197 (MQ=255)                                                                               gtagATCATTGCCGTTACGTTCAAATGACACCTCTTTGAGGTTGATATTTGCTAATGATAATTTATCTTTATCACCGCTATGTTCCGTAATAGTGTGGTGGCCATATTCCTTACGGTAAACATAAATATCATTTCCTTCTCCACCGGCAAGTTGATCATCACCTTTTCCGCCATCAAGCAAGTCATCTCCTTGGTTTCCAAATAATTTATCATTGCCACTTCCGCCTCTTAGAATATCGGAACCAttccc                                                                                                      >  1:175079/1‑247 (MQ=255)                                                                               gtagATCATTGCCGTTACGTTCAAATGACACCTCTTTGAGGTTGATATTTGCTAATGATAATTTATCTTTATCACCGCTATGTTCCGTAATAGTGTGGTGGCCATATTCCTTACGGTAAACATAAATATCATTTCCTTCTCCACCGGCAAGTTGATCATCACCTTTTCCGCCATCAAGCAAGTCATCTCCTTGGTTTCCAAATAATTTATCATTGCCACTTCCGCCTCTTAGAATATCGGAACCAttccc                                                                                                      >  1:383459/1‑247 (MQ=255)                                                                                tagATCATTGCCGTTACGTTCAAATGACACCTCTTTGAGGTTGATATTTGCTAATGATAATTTATCTTTATCACCGCTATGTTCCGTAATAGTGTGGTGGCCATATTCCTTACGGTAAACATAAATATCATTTCCTTCTCCACCGGCAAGTTGATCATCACCTTTTCCGCCATCAAGCAAGTCATCTCCTTGGTTTCCAAATAATTTATCATTGCCACTTCCGCCTCTTAGAATATCGGAACCAttccccc                                                                                                    <  1:78416/251‑6 (MQ=255)                                                                                          ccGTTACGTTCAAATGACACCTCTTTGAGGTTGATATTTGCTAATGATAATTTATCTTTATCACCGCTATGTTCCGTAATAGTGTGGTGGCCATATTCCTTACGGTAAACATAAATATCATTTCCTTCTCCACCGGCAAGTTGATCATCACCTTTTCCGCCATCAAGCAAGTCATCTCCTTGGTTTCCAAATAATTTATCATTGCCACTTCCGCCTCTTAGAATATCGGAACCATTCCCCCCTTCTaaat                                                                                           <  1:510923/250‑3 (MQ=255)                                                                                                   tCAAATGACACCTCTTTGAGGTTGATATTTGCTAATGATAATTTATCTTTATCACCGCTATGTTCCGTAATAGTGTGGTGGCCATATTCCTTACGGTAAACATAAATATCATTTCCTTCTCCACCGGCAAGTTGATCATCACCTTTTCCGCCATCAAGCAAGTCATCTCCTTGGTTTCCAAATAATTTATCATTGCCACTTCCGCCTCTTAGAATATCGGAACCATTCCCCCCTTCTAAATGGTCGTCGcc                                                                                 <  1:825617/251‑1 (MQ=255)                                                                                                          acacCTCTTTGAGGTTGATATTTGCTAATGATAATTTATCTTTATCACCGCTATGTTCCGTAATAGTGTGGTGGCCATATTCCTTACGGTAAACATAAATATCATTTCCTTCTCCACCGGCAAGTTGATCATCACCTTTTCCGCCATCGAGCAAGTCATCTCCTTGGTTTCCAAATAATTTATCATTGCCACTTCCGCCTCTTAGAATATCGGAACCATTCCCCCCTTCTAAATGGTCGTCGCCTTCACCa                                                                          <  1:19770/251‑1 (MQ=255)                                                                                                          acacCTCTTTGAGGTTGATATTTGCTAATGATAATTTATCTTTATCACCGCTATGTTCCGTAATAGTGTGGTGGCCATATTCCTTACGGTAAACATAAATATCATTTCCTTCTCCACCGGCAAGTTGATCATCACCTTTTCCGCCATCAAGCAAGTCATCTCCTTGGTTTCCAAATAATTTATCATTGCCACTTCCGCCTCTTAGAATATCGGAACCATTCCCCCCTTCTAAATGGTCGTCGCCTtcacc                                                                           <  1:47213/250‑4 (MQ=255)                                                                                                              ctCTTTGAGGTTGATATTTGCTAATGATAATTTATCTTTATCACCGCTATGTTCCGTAATAGTGTGGTGGCCATATTCCTTACGGTAAACATAAATATCATTTCCTTCTCCACCGGCAAGTTGATCATCACCTTTTCCGCCATCAAGCAAGTCATCTCCTTGGTTTCCAAATAATTTATCATTGCCACTTCCGCCTCTTAGAATATCGGAACCATTCCCCCCTTCTAAATGGTCGTCGCCTtcacc                                                                           <  1:413163/245‑4 (MQ=255)                                                                                                                cTTTGAGGTTGATATTTGCTAATGATAATTTATCTTTATCACCGCTATGTTCCGTAATAGTGTGGTGGCCATATTCCTTACGGTAAACATAAATATCATTTCCTTCTCCACCGGCAAGTTGATCATCACCTTTTCCGCCATCAAGCAAGTCATCTCCTTGGTTTCCAAATAATTTATCATTGCCACTTCCGCCTCTTAGAATATCGGAACCATTCCCCCCTTCTAAATGGTCGTCGCCTTCACCACCGt                                                                      >  1:24648/1‑249 (MQ=255)                                                                                                                cTTTGAGGTTGATATTTGCTAATGATAATTTATCTTTATCACCGCTATGTTCCGTAATAGTGTGGTGGCCATATTCCTTACGGTAAACATAAATATCATTTCCTTCTCCACCGGCAAGTTGATCATCACCTTTTCCGCCATCAAGCAAGTCATCTCCTTGGTTTCCAAATAATTTATCATTGCCACTTCCGCCTCTTAGAATATCGGAACCATTCCCCCCTTCTAAATGGTCGTCGCCTTCACCACCGt                                                                      >  1:676970/1‑249 (MQ=255)                                                                                                                  ttGAGGTTGATATTTGCTAATGATAATTTATCTTTATCACCGCTATGTTCCGTAATAGTGTGGTGGCCATATTCCTTACGGTAAACATAAATATCATTTCCTTCTCCACCGGCAAGTTGATCATCACCTTTTCCGCCATCAAGCAAGTCATCTCCTTGGTTTCCAAATAATTTATCATTGCCACTTCCGCCTCTTAGAATATCGGAACCATTCCCCCCTTCTAAATGGTCGTCGCCTTCACCACCGTctag                                                                  <  1:92271/251‑4 (MQ=255)                                                                                                                     aggttGATATTTGCTAATGATAATTTATCTTTATCACCGCTATGTTCCGTAATAGTGTGGTGGCCATATTCCTTACGGTAAACATAAATATCATTTCCTTCTCCACCGGCAAGTTGATCATCACCTTTTTCGCCATCAAGCAAGTCATCTCCTTGGTTTCCAAATAATTTATCATTGCCACTTCCGCCTCTTAGAATATCGGAACCATTCCCCCCTTCTAAATGGTCGTCGCCTTCACCACCGTCTAGAt                                                                <  1:178795/247‑1 (MQ=255)                                                                                                                     aggttGATATTTGCTAATGATAATTTATCTTTATCACCGCTATGTTCCGTAATAGTGTGGTGGCCATATTCCTTACGGTAAACATAAATATCATTTCCTTCTCCACCGGCAAGTTGATCATCACCTTTTCCGCCATCAAGCAAGTCATCTCCTTGGTTTCCAAATAATTTATCATTGCCACTTCCGCCTCTTAGAATATCGGAACCATTCCCCCCTTCTAAATGGTCGTCGCCTTCACCACCGTCTAGAt                                                                <  1:604227/247‑1 (MQ=255)                                                                                                                     aggttGATATTTGCTAATGATAATTTATCTTTATCACCGCTATGTTCCGTAATAGTGTGGTGGCCATATTCCTTACGGTAAACATAAATATCATTTCCTTCTCCACCGGCAAGTTGATCATCACCTTTTCCGCCATCAAGCAAGTCATCTCCTTGGTTTCCAAATAATTTATCATTGCCACTTCCGCCTCTTAGAATATCGGAACCATTCCCCCCTTCTAAATGGTCGTCGCCTTCACCACCGTCTAGAt                                                                <  1:461324/247‑1 (MQ=255)                                                                                                                     aggttGATATTTGCTAATGATAATTTATCTTTATCACCGCTATGTTCCGTAATAGTGTGGTGGCCATATTCCTTACGGTAAACATAAATATCATTTCCTTCTCCACCGGCAAGTTGATCATCACCTTTTCCGCCATCAAGCAAGTCATCTCCTTGGTTTCCAAATAATTTATCATTGCCACTTCCGCCTCTTAGAATATCGGAACCATTCCCCCCTTCTAAATGGTCGTCGCCTTCACCACCGTCTAGAt                                                                <  1:793025/247‑1 (MQ=255)                                                                                                                       gttGATATTTGCTAATGATAATTTATCTTTATCACCGCTATGTTCCGTAATAGTGTGGTGGCCATATTCCTTACGGTAAACATAAATATCATTTCCTTCTCCACCGGCAAGTTGATCATCACCTTTTCCGCCATCAAGCAAGTCATCTCCTTGGTTTCCAAATAATTTATCATTGCCACTTCCGCCTCTTAGAATATCGGAACCATTCCCCCCTTCTAAATGGTCGTCGCCTTCACCACCGTCTAGAt                                                                >  1:672489/2‑248 (MQ=255)                                                                                                                         tGATATTTGCTAATGATAATTTATCTTTATCACCGCTATGTTCCGTAATAGTGTGGTGGCCATATTCCTTACGGTAAACATAAATATCATTTCCTTCTCCACCGGCAAGTTGATCATCACCTTTTCCGCCATCAAGCAAGTCATCTCCTTGGTTTCCAAATAATTTATCATTGCCACTTCCGCCTCTTAGAATATCGGAACCATTCCCCCCTTCTAAATGGTCGTCGCCTTCACCACCGTCTAGATAgttg                                                           <  1:236144/251‑1 (MQ=255)                                                                                                                                  cTAATGATAATTTATCTTTATCACCGCTATGTTCCGTAATAGTGTGGTGGCCATATTCCTTACGGTAAACATAAATATCATTTCCTTCTCCACCGGCAAGTTGATCATCACCTTTTCCGCCATCAAGCAAGTCATCTCCTTGGTTTCCAAATAATTTATCATTGCCACTTCCGCCTCTTAGAATATCGGAACCATTCCCCCCTTCTAAATGGTCGTCGCCTTCACCACCGTCTAGATAGTTGTTACCatat                                                  <  1:600827/251‑1 (MQ=255)                                                                                                                                         tAATTTATCTTTATCACCGCTATGTTCCGTAATAGTGTGGTGGCCATATTCCTTACGGTAAACATAAATATCATTTCCTTCTCCACCGGCAAGTTGATCATCACCTTTTCCGCCATCAAGCAAGTCATCTCCTTGGTTTCCAAATAATTTATCATTGCCACTTCCGCCTCTTAGAATATCGGAACCATTCCCCCCTTCTAAATGGTCGTCGCCTTCACCACCGTCTAGATAGTTGTTACCATATTCACCaa                                           <  1:660471/251‑1 (MQ=255)                                                                                                                                           aTTTATCTTTATCACCGCTATGTTCCGTAATAGTGTGGTGGCCATATTCCTTACGGTAAACATAAATATCATTTCCTTCTCCACCGGCAAGTTGATCATCACCTTTTCCGCCATCAAGCAAGTCATCTCCTTGGTTTCCAAATAATTTATCATTGCCACTTCCGCCTCTTAGAATATCGGAACCATTCCCCCCTTCTAAATGGTCGTCGCCTTCACCACCGTCTAGATAGTTGTTACCATATTCACCAAag                                         <  1:549865/251‑1 (MQ=255)                                                                                                                                                      tcACCGCTATGTTCCGTAATAGTGTGGTGGCCATATTCCTTACGGTAAACATAAATATCATTTCCTTCTCCACCGGCAAGTTGATCATCACCTTTTCCGCCATCAAGCAAGTCATCTCCTTGGTTTCCAAATAATTTATCATTGCCACTTCCGCCTCTTAGAATATCGGAACCATTCCCCCCTTCTAAATgg                                                                                         <  1:395947/192‑1 (MQ=255)                                                                                                                                                         ccGCTATGTTCCGTAATAGTGTGGTGGCCATATTCCTTACGGTAAACATAAATATCATTTCCTTCTCCACCGGCAAGTTGATCATCACCTTTTCCGCCATCAAGCAAGTCATCTCCTTGGTTTCCAAATAATTTATCATTGCCACTTCCGCCTCTTAGAATATCGGAACCATTCCCCCCTTCTaaat                                                                                           <  1:29080/187‑3 (MQ=255)                                                                                                                                                         ccGCTATGTTCCGTAATAGTGTGGTGGCCATATTCCTTACGGTAAACATAAATATCATTTCCTTCTCCACCGGCAAGTTGATCATCACCTTTTCCGCCATCAAGCAAGTCATCTCCTTGGTTTCCAAATAATTTATCATTGCCACTTCCGCCTCTTAGAATATCGGAACCATTCCCCCCTTCTAAATGGTCGTCGCCTTcaccac                                                                         >  1:895642/1‑205 (MQ=255)                                                                                                                                                         ccGCTATGTTCCGTAATAGTGTGGTGGCCATATTCCTTACGGTAAACATAAATATCATTTCCTTCTCCACCGGCAAGTTGATCATCACCTTTTCCGCCATCAAGCAAGTCATCTCCTTGGTTTCCAAATAATTTATCATTGCCACTTCCGCCTCTTAGAATATCGGAACCATTCCCCCCTTCTAAATGGTCGTCGCCTTCACCACCGTCTAGATAGTTGTTACCATATTCACCAAAGAGCCtgtcattgc                            >  1:385254/1‑242 (MQ=255)                                                                                                                                                              aTGTTCCGTAATAGTGTGGTGGCCATATTCCTTACGGTAAACATAAATATCATTTCCTTCTCCACCGGCAAGTTGATCATCACCTTTTCCGCCATCAAGCAAGTCATCTCCTTGGTTTCCAAATAATTTATCATTGCCACTTCCGCCTCTTAGAATATCGGAACCATTCCCCCCTTCTAAATGGTCGTCGCCTTCACCACCGTCTAGATAgttg                                                           <  1:419764/214‑1 (MQ=255)                                                                                                                                                                gTTCCGTAATAGTGTGGTGGCCATATTCCTTACGGTAAACATAAATATCATTTCCTTCTCCACCGGCAAGTTGATCATCACCTTTTCCGCCATCAAGCAAGTCATCTCCTTGGTTTCCAAATAATTTATCATTGCCACTTCCGCCTCTTAGAATATCGGAACCAttcccc                                                                                                     >  1:840479/1‑166 (MQ=255)                                                                                                                                                                gTTCCGTAATAGTGTGGTGGCCATATTCCTTACGGTAAACATAAATATCATTTCCTTCTCCACCGGCAAGTTGATCATCACCTTTTCCGCCATCAAGCAAGTCATCTCCTTGGTTTCCAAATAATTTATCATTGCCACTTCCGCCTCTTAGAATATCGGAACCATTCCCCCCTTCTAAATGGTCGTCGCCTTCACCACCGTCTAGATAGTTGTTACCATATTCACCAAAGAGCCTGTCATTGCCGGcgcca                    >  1:527365/1‑251 (MQ=255)                                                                                                                                                                 ttCCGTAATAGTGTGGTGGCCATATTCCTTACGGTAAACATAAATATCATTTCCTTCTCCACCGGCAAGTTGATCATCACCTTTTCCGCCATCAAGCAAGTCATCTCCTTGGTTTCCAAATAATTTATCATTGCCACTTCCGCCTCTTAGAATATCGGAACCATTCCCCCCTTCTAAATGGTCGTCGCCTTcaccac                                                                         >  1:654430/1‑197 (MQ=255)                                                                                                                                                                   ccGTAATAGTGTGGTGGCCATATTCCTTACGGTAAACATAAATATCATTTCCTTCTCCACCGGCAAGTTGATCATCACCTTTTCCGCCATCAAGCAAGTCATCTCCTTGGTTTCCAAATAATTTATCATTGCCACTTCCGCCTCTTAGAATATCGGAACCATTCCCCCCTTCTAAATGGTCGTCGCCTTCACCACCGTCTAGATAGTTGTTACCat                                                    >  1:843668/1‑216 (MQ=255)                                                                                                                                                                   ccGTAATAGTGTGGTGGCCATATTCCTTACGGTAAACATAAATATCATTTCCTTCTCCACCGGCAAGTTGATCATCACCTTTTCCGCCATCAAGCAAGTCATCTCCTTGGTTTCCAAATAATTTATCATTGCCACTTCCGCCTCTTAGAATATCGGAACCATTCCCCCCTTCTAAATGGTCGTCGCCTTCACCACCGTCTAGATAGTTGTTACCATATTCACCAAAGAGCCTGTCATTGCCGGCGccacca                 >  1:667289/1‑251 (MQ=255)                                                                                                                                                                   ccGTAATAGTGTGGTGGCCATATTCCTTACGGTAAACATAAATATCATTTCCTTCTCCACCGGCAAGTTGATCATCACCTTTTCCGCCATCAAGCAAGTCATCTCCTTGGTTTCCAAATAATTTATCATTGCCACTTCCGCCTCTTAGAATATCGGAACCATTCCCCCCTTCTAAATGGTCGTCGCCTTCACCACCGTCTAGATAGTTGTTACCATATTCACCAAAGAGCCTGTCATTGCCGGCGccacca                 >  1:540493/1‑251 (MQ=255)                                                                                                                                                                   ccGTAATAGTGTGGTGGCCATATTCCTTACGGTAAACATAAATATCATTTCCTTCTCCACCGGCAAGTTGATCATCACCTTTTCCGCCATCAAGCAAGTCATCTCCTTGGTTTCCAAATAATTTATCATTGCCACTTCCGCCTCTTAGAATATCGGAACCATTCCCCCCTTCTAAATGGTCGTCGCCTTCACCACCGTCTAGATAGTTGTTACCATATTCACCAAAGAGCCTGTCATTGCCGGCGccacca                 >  1:154513/1‑251 (MQ=255)                                                                                                                                                                     gTAATAGTGTGGTGGCCATATTCCTTACGGTAAACATAAATATCATTTCCTTCTCCACCGGCAAGTTGATCATCACCTTTTCCGCCATCAAGCAAGTCATCTCCTTGGTTTCCAAATAATTTATCATTGCCACTTCCGCCTCTTAGAATATCGGAACCAttccccccttc                                                                                                >  1:240867/1‑161 (MQ=255)                                                                                                                                                                     gTAATAGTGTGGTGGCCATATTCCTTACGGTAAACATAAATATCATTTCCTTCTCCACCGGCAAGTTGATCATCACCTTTTCCGCCATCAAGCAAGTCATCTCCTTGGTTTCCAAATAATTTATCATTGCCACTTCCGCCTCTTAGAATATCGGAACCAttcccccc                                                                                                   <  1:355894/167‑7 (MQ=255)                                                                                                                                                                     gTAATAGTGTGGTGGCCATATTCCTTACGGTAAACATAAATATCATTTCCTTCTCCACCGGCAAGTTGATCATCACCTTTTCCGCCATCAAGCAAGTCATCTCCTTGGTTTCCAAATAATTTATCATTGCCACTTCCGCCTCTTAGAATATCGGAACCATTCCCCCCTTCTaaa                                                                                            >  1:246988/1‑173 (MQ=255)                                                                                                                                                                        aTAGTGTGGTGGCCATATTCCTTACGGTAAACATAAATATCATTTCCTTCTCCACCGGCAAGTTGATCATCACCTTTTCCGCCATCAAGCAAGTCATCTCCTTGGTTTCCAAATAATTTATCATTGCCACTTCCGCCTCTTAGAATATCGGAACCATTCCCCCCTTCTAAATGGTCGTCGCCTTCACCACCGTCTAGATAGTTGTTACCATATTCACCAAagag                                       <  1:113284/224‑1 (MQ=255)                                                                                                                                                                        aTAGTGTGGTGGCCATATTCCTTACGGTAAACATAAATATCATTTCCTTCTCCACCGGCAAGTTGATCATCACCTTTTCCGCCATCAAGCAAGTCATCTCCTTGGTTTCCAAATAATTTATCATTGCCACTTCCGCCTCTTAGAATATCGGAACCATTCCCCCCTTCTAAATGGTCGTCGCCTTCACCACCGTCTAGATAGTTGTTACCATATTCACCAAAGAGCCtgtcattg                             <  1:439535/234‑8 (MQ=255)                                                                                                                                                                        aTAGTGTGGTGGCCATATTCCTTACGGTAAACATAAATATCATTTCCTTCTCCACCGGCAAGTTGATCATCACCTTTTCCGCCATCAAGCAAGTCATCTCCTTGGTTTCCAAATAATTTATCATTGCCACTTCCGCCTCTTAGAATATCGGAACCATTCCCCCCTTCTAAATGGTCGTCGCCTTCACCACCGTCTAGATAGTTGTTACCATATTCACCAAAGAGCCTGTCATTGCCGGCGCCACCATaaag            <  1:86129/251‑4 (MQ=255)                                                                                                                                                                        aTAGTGTGGTGGCCATATTCCTTACGGTAAACATAAATATCATTTCCTTCTCCACCGGCAAGTTGATCATCACCTTTTCCGCCATCAAGCAAGTCATCTCCTTGGTTTCCAAATAATTTATCATTGCCACTTCCGCCTCTTAGAATATCGGAACCATTCCCCCCTTCTAAATGGTCGTCGCCTTCACCACCGTCTAGATAGTTGTTACCATATTCACCAAAGAGCCTGTCATTGCCGGCGCCACCATaaag            >  1:319844/1‑248 (MQ=255)                                                                                                                                                                         tAGTGTGGTGGCCATATTCCTTACGGTAAACATAAATATCATTTCCTTCTCCACCGGCAAGTTGATCATCACCTTTTCCGCCATCAAGCAAGTCATCTCCTTGGTTTCCAAATAATTTATCATTGCCACTTCCGCCTCTTAGAATATCGGAACCATTCCCCCCTTCTAAATGGTCGTCGCCTTCACCACCGTCTAGATAGTTGTTACCATATTCACCAAAGAGCCTGTCATTGCCGGCGCCACCATaaagt           <  1:482596/251‑5 (MQ=255)                                                                                                                                                                          aGTGTGGTGGCCATATTCCTTACGGTAAACATAAATATCATTTCCTTCTCCACCGGCAAGTTGATCATCACCTTTTCCGCCATCAAGCAAGTCATCTCCTTGGTTTCCAAATAATTTATCATTGCCACTTCCGCCTCTTAGAATATCGGAACCATTCCCCCCTTCTAAATGGTCGTCGCCTTCACCACCGTCTAGATAGTTGTTACCATATTCACCAAAGAGCCTGTCATTGCCGGCGCCACCATaaagtt          >  1:289420/1‑246 (MQ=255)                                                                                                                                                                           gtgtGGTGGCCATATTCCTTACGGTAAACATAAATATCATTTCCTTCTCCACCGGCAAGTTGATCATCACCTTTTCCGCCATCAAGCAAGTCATCTCCTTGGTTTCCAAATAATTTATCATTGCCACTTCCGCCTCTTAGAATATCGGAACCATTCCCCCCTTCTAAATGGTCGTCGCCTTCACCACCGTCTAGATAGTTGTTACCATATTCACCAAAGAGCCTGTCATTGCCGGCGCCACCATaaagttt         >  1:79381/1‑245 (MQ=255)                                                                                                                                                                             gtggtgGCCATATTCCTTACGGTAAACATAAATATCATTTCCTTCTCCACCGGCAAGTTGATCATCACCTTTTCCGCCATCAAGCAAGTCATCTCCTTGGTTTCCAAATAATTTATCATTGCCACTTCCGCCTCTTAGAATATCGGAACCATTCCCCCCTTCTAAATGGTCGTCGCCTTCACCACCGTCTAGATAGTTGTTACCata                                                   >  1:163873/1‑207 (MQ=255)                                                                                                                                                                               ggtgGCCATATTCCTTACGGTAAACATAAATATCATTTCCTTCTCCACCGGCAAGTTGATCATCACCTTTTCCGCCATCAAGCAAGTCATCTCCTTGGTTTCCAAATAATTTATCATTGCCACTTCCGCCTCTTAGAATATCGGAACCATTCCCCCCTTCTAAATGGTCGTCGCCTTCACCACCGTCTAGATAGTTGTTACCATATTCACCAAAGAGCCTGTCATTGCCGGCGCCACCATaaagtttgtcg     >  1:611300/1‑241 (MQ=255)                                                                                                                                                                                  ggccATATTCCTTACGGTAAACATAAATATCATTTCCTTCTCCACCGGCAAGTTGATCATCACCTTTTCCGCCATCAAGCAAGTCATCTCCTTGGTTTCCAAATAATTTATCATTGCCACTTCCGCCTCTTAGAATATCGGAACCATTCCCCCCTTCTAAATGGTCGTCGCCTTCACCACCGTCTAGATAGTTGTTACCATATTCACCAAAGAGCCTGTCATTGCCGGCGCCACCATaaagtttgtcgttg  >  1:340776/3‑238 (MQ=255)                                                                                                                                                                                   gccATATTCCTTACGGTAAACATAAATATCATTTCCTTCTCCACCGGCAAGTTGATCATCACCTTTTCCGCCATCAAGCAAGTCATCTCCTTGGTTTCCAAATAATTTATCATTGCCACTTCCGCCTCTTAGAATATCGGAAc                                                                                                             >  1:787529/2‑143 (MQ=255)                                                                                                                                                                                   gccATATTCCTTACGGTAAACATAAATATCATTTCCTTCTCCACCGGCAAGTTGATCATCACCTTTTCCGCCATCAAGCAAGTCATCTCCTTGGTTTCCAAATAATTTATCATTGCCACTTCCGCCTCTTAGAATATCGGAACCATTCCCCCCTTCTAAATGGTCGTCGCCTTCACCACCGTCTAGAt                                                                <  1:135531/187‑1 (MQ=255)                                                                                                                                                                                   gccATATTCCTTACGGTAAACATAAATATCATTTCCTTCTCCACCGGCAAGTTGATCATCACCTTTTCCGCCATCAAGCAAGTCATCTCCTTGGTTTCCAAATAATTTATCATTGCCACTTCCGCCTCTTAGAATATCGGAACCATTCCCCCCTTCTAAATGGTCGTCGCCTTCACCACCGTCTAGATAGTTGTTACCATATTCACCAAAGAGCCTGTCATTGCCGGCGCCACCATaaagtttgtcgttg  >  1:226947/2‑237 (MQ=255)                                                                                                                                                                                   gccATATTCCTTACGGTAAACATAAATATCATTTCCTTCTCCACCGGCAAGTTGATCATCACCTTTTCCGCCATCAAGCAAGTCATCTCCTTGGTTTCCAAATAATTTATCATTGCCACTTCCGCCTCTTAGAATATCGGAACCATTCCCCCCTTCTAAATGGTCGTCGCCTTCACCACCGTCTAGATAGTTGTTACCATATTCACCAAAGAGCCTGTCATTGCCGGCGCCACCATaaagtttgtcgttg  >  1:596773/2‑237 (MQ=255)                                                                                                                                                                                        atTCCTTACGGTAAACATAAATATCATTTCCTTCTCCACCGGCAAGTTGATCATCACCTTTTCCGCCATCAAGCAAGTCATCTCCTTGGTTTCCAAATAATTTATCTTTGCCACTTCCGCCTCTTAGAATATCGGAACCATTCCCCCCTTCTAAATGGTCGTCGCCTTCACCACCGTCTAGATAGTTGTTACCATATTCACCaa                                           >  1:760071/1‑204 (MQ=255)                                                                                                                                                                                           ccttACGGTAAACATAAATATCATTTCCTTCTCCACCGGCAAGTTGATCATCACCTTTTCCGCCATCAAGCAAGTCATCTCCTTGGTTTCCAAATAATTTATCATTGCCACTTCCGCCTCTTAGAATATCGGAAc                                                                                                             >  1:868992/3‑135 (MQ=255)                                                                                                                                                                                           ccttACGGTAAACATAAATATCATTTCCTTCTCCACCGGCAAGTTGATCATCACCTTTTCCGCCATCAAGCAAGTCATCTCCTTGGTTTCCAAATAATTTATCATTGCCACTTCCGCCTCTTAGAATATCGGAACCATTCCCCCCTTCTAAATgg                                                                                         >  1:890113/3‑155 (MQ=255)                                                                                                                                                                                                      aCATAAATATCATTTCCTTCTCCACCGGCAAGTTGATCATCACCTTTTCCGCCATCAAGCAAGTCATCTCCTTGGTTTCCAAATAATTTATCATTGCCACTTCCGCCTCTTAGAATATCGGAACCATTCCCCCCTTCTAAATGGTCGTCGCCTTCACCACCGTCTAGATAGTTGTTACCATATTCACCAAAGAGCCtgtcattgcc                           >  1:669250/1‑197 (MQ=255)                                                                                                                                                                                                       cATAAATATCATTTCCTTCTCCACCGGCAAGTTGATCATCACCTTTTCCGCCATCAAGCAAGTCATCTCCTTGGTTTCCAAATAATTTATCATTGCCACTTCCGCCTCTTAGAATATCGGAACCAttccccccttcta                                                                                              <  1:574100/138‑12 (MQ=255)                                                                                                                                                                                                                                               |                                                                                                                                                                                              CATCTAATCCTGCCGATGAATTAGGTTTACTAAACCATCCTTTAAATGTTACTGCTGTTCTATTATTTGTTTTCAATAGTAGATCATTGCCGTTACGCTCAAATGACACATCTTTGAGATTGATATTTGCTAATGATAATTTATCTTTATCACCGCTATGTTCCGTAATAGTGTGGTGCCCATATTCTTTACGGTAAACATAAATATCATTTCCTTCTCCACCGGCAAGTTGGTCATCGCCTTCTCCACCGTCAAGTAAATCATCTCCTTGGTTTCCAAACAACTTATCATTGCCACTTCCACCTCTTAGAATATCGGAACCATTGCCTCCCTCTAAGTGGTCGTCGCCTTCTCCACCGTCAAGATAGTTGTTGCCATATTCACCAAAGAGCCTATCGTTACCGGCACCACCATAGAGCTTATCATTA  >  1/1979390‑1979817

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑