Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,979,393	C→T	D912N (GAT→AAT)	unknown ←	RTX toxins determinant A and related Ca2+‑binding proteins

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,979,393	0	C	T	100.0%	187.4 / NA	66	D912N (GAT→AAT)	unknown	RTX toxins determinant A and related Ca2+‑binding proteins
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (31/35);  total (31/35)

CCTTTATTCTTAATAAGTCCGGCAAGCTCTTGAGGTGAAATTGTCTTTTTGTTCCCACTCTTATCAAAAAGATTATCAATGTCTTGCGAAGTAATCCGCTCCCCATCTTTACCGATAATTTCTTCAACTTTATTATTGAGTGATTTGTCGACTTTACCTCGCTGTAACTCAAATTGTCTCTTAAGTCGTGCACGATCCTTTTCAGGTGCGTATTCAAGAAGTTTTCTTTGATACTCATCTAATCCTGCCGATGAATTAGGTTTACTAAACCATCCTTTAAATGTTACTGCTGTTCTATTATTTGTTTTCAATAGTAGATCATTGCCGTTACGCTCAAATGACACATCTTTGAGATTGATATTTGCTAATGATAATTTATCTTTATCACCGCTATGTTCCGTAATAGTGTGGTGCCCATATTCTTTACGGTAAACATAAATATCAT  >  1/1979155‑1979599                                                                                                                                                                                                                                               |                                                                                                                                                                                                               ccTTTATTCTTAATAAGCCCGGCAAGCTCTTGAGGTGAAATTGTCTTTTTATTCCCACTTTTATCAAGAAGATTATCAATGTCTTGCGAAGTAATCCGCTCCCCATCTTTACCGATAATTTCTTCAACTTTATTATTGAGTGATTTGTCGACTTTACCTCGCTGTAACTCAAATTGTCTCTTAAGTCGTGCACGATCCTTTTCAGGTGCGTATTCAAGAAGTTTTCTTTGATACTCATTTAACCCCGCCGa                                                                                                                                                                                                    >  1:647766/1‑251 (MQ=255)       ttCTTAATAAGCCCGGCAAGCTCTTGAGGTGAAATTGTCTTTTTATTCCCACTTTTATCAAGAAGATTATCAATGTCTTGCGAAGTAATCCGCTCCCCATCTTTACCGATAATTTCTTCAACTTTATTATTGAGTGATTTGTCGACTTTACCTCGCTGTAACTCAAATTGTCTCTTAAGTCGTGCACGATCCTTTTCAGGTGCGTATTCAAGAAGTTTTCTTTGATACTCATTTAACCCCGCCGACGAAtt                                                                                                                                                                                              >  1:285819/1‑251 (MQ=255)                  cccGGCAAGCTCTTGAGGTGAAATTGTCTTTTTATTCCCACTTTTATCAAGAAGATTATCAATGTCTTGCGAAGTAATCCGCTCCCCATCTTTACCGATAATTTCTTCAACTTTATTATTGAGTGATTTGTCGACTTTACCTCGCTGTAACTCAAATTGTCTCTTAAGTCGTGCACGATCCTTTTCAGGTGCGTATTCAAGAAGTTTTCTTTGATACTCATTTAACCCCGCCGACGAATTAGGTTTatta                                                                                                                                                                                    <  1:101569/249‑4 (MQ=255)                    cGGCAAGCTCTTGAGGTGAAATTGTCTTTTTATTCCCACTTTTATCAAGAAGATTATCAATGTCTTGCGAAGTAATCCGCTCCCCATCTTTACCGATAATTTCTTCAACTTTATTATTGAGTGATTTGTCGACTTTACCTCGCTGTAACTCAAATTGTCTCTTAAGTCGTGCACGATCCTTTTCAGGTGCGTATTCAAGAAGTTTTCTTTGATACTCATTTAACCCCGCCGACGAATTAGGTTTATTAAAc                                                                                                                                                                                 <  1:155929/251‑1 (MQ=255)                    cGGCAAGCTCTTGAGGTGAAATTGTCTTTTTATTCCCACTTTTATCAAGAAGATTATCAATGTCTTGCGAAGTAATCCGCTCCCCATCTTTACCGATAATTTCTTCAACTTTATTATTGAGTGATTTGTCGACTTTACCTCGCTGTAACTCAAATTGTCTCTTAAGTCGTGCACGATCCTTTTCAGGTGCGTATTCAAGAAGTTTTCTTTGATACTCATTTAACCCCGCCGACGAATTAGGTTTATTAAAc                                                                                                                                                                                 <  1:513418/251‑1 (MQ=255)                      gCAAGCTCTTGAGGTGAAATTGTCTTTTTATTCCCACTTTTATCAAGAAGATTATCAATGTCTTGCGAAGTAATCCGCTCCCCATCTTTACCGATAATTTCTTCAACTTTATTATTGAGTGATTTGTCGACTTTACCTCGCTGTAACTCAAATTGTCTCTTAAGTCGTGCACGATCCTTTTCAGGTGCGTATTCAAGAAGTTTTCTTTGATACTCATTTAACCCCGCCGACGAATTAGGTTTATTAAAcc                                                                                                                                                                                <  1:755845/250‑1 (MQ=255)                          gCTCTTGAGGTGAAATTGTCTTTTTATTCCCACTTTTATCAAGAAGATTATCAATGTCTTGCGAAGTAATCCGCTCCCCATCTTTACCGATAATTTCTTCAACTTTATTATTGAGTGATTTGTCGACTTTACCTCGCTGTAACTCAAATTGTCTCTTAAGTCGTGCACGATCCTTTTCAGGTGCGTATTCAAGAAGTTTTCTTTGATACTCATTTAACCCCGCCGACGAATTAGGTTTATTAAACCATc                                                                                                                                                                             >  1:513062/1‑249 (MQ=255)                           ctctTGAGGTGAAATTGTCTTTTTATTCCCACTTTTATCAAGAAGATTATCAATGTCTTGCGAAGTAATCCGCTCCCCATCTTTACCGATAATTTCTTCAACTTTATTATTGAGTGATTTGTCGACTTTACCTCGCTGTAACTCAAATTGTCTCTTAAGTCGTGCACGATCCTTTTCAGGTGCGTATTCAAGAAGTTTTCTTTGATACTCATTTAACCCCGCCGACGAATTAGGTTTATTAAACCATCCtt                                                                                                                                                                          >  1:715767/1‑251 (MQ=255)                               tGAGGTGAAATTGTCTTTTTATTCCCACTTTTATCAAGAAGATTATCAATGTCTTGCGAAGTAATCCGCTCCCCATCTTTACCGATAATTTCTTCAACTTTATTATTGAGTGATTTGTCGACTTTACCTCGCTGTAACTCAAATTGTCTCTTAAGTCGTGCACGATCCTTTTCAGGTGCGTATTCAAGAAGTTTTCTTTGATACTCATTTAACCCCGCCGACGAATTAGGTTTATTAAACCATCCTTTaaa                                                                                                                                                                      <  1:358899/251‑1 (MQ=255)                                   gTGAAATTGTCTTTTTATTCCCACTTTTATCAAGAAGATTATCAATGTCTTGCGAAGTAATCCGCTCCCCATCTTTACCGATAATTTCTTCAACTTTATTATTGAGTGATTTGTCGACTTTACCTCGCTGTAACTCAAATTGTCTCTTAAGTCGTGCACGATCCTTTTCAGGTGCGTATTCAAGAAGTTTTCTTTGATACTCATTTAACCCCGCCGACGAATTAGGTTTATTAAACCATCCTTTAAATg                                                                                                                                                                    <  1:782172/249‑1 (MQ=255)                                           gTCTTTTTATTCCCACTTTTATCAAGAAGATTATCAATGTCTTGCGAAGTAATCCGCTCCCCATCTTTACCGATAATTTCTTCAACTTTATTATTGAGTGATTTGTCGACTTTACCTCGCTGTAACTCAAATTGTCTCTTAAGTCGTGCACGATCCTTTTCAGGTGCGTATTCAAGAAGTTTTCTTTGATACTCATTTAACCCCGCCGACGAATTAGGTTTATTAAACCATCCTTTAAATGTTActg                                                                                                                                                              >  1:253655/1‑247 (MQ=255)                                               ttttATTCCCACTTTTATCAAGAAGATTATCAATGTCTTGCGAAGTAATCCGCTCCCCATCTTTACCGATAATTTCTTCAACTTTATTATTGAGTGATTTGTCGACTTTACCTCGCTGTAACTCAAATTGTCTCTTAAGTCGTGCACGATCCTTTTCAGGTGCGTATTCAAGAAGTTTTCTTTGATACTCATTTAACCCCGCCGACGAATTAGGt                                                                                                                                                                                          >  1:214301/1‑215 (MQ=255)                                                  tattCCCACTTTTATCAAGAAGATTATCAATGTCTTGCGAAGTAATCCGCTCCCCATCTTTACCGATAATTTCTTCAACTTTATTATTGAGTGATTTGTCGACTTTACCTCGCTGTAACTCAAATTGTCTCTTAAGTCGTGCACGATCCTTTTCAGGTGCGTATTCAAGAAGTTTTCTTTGATACTCATTTAACCCCGCCGACGAATTAGGTTTATTAAACCATCCTTTAAATGTTACTGCTGTTCtatta                                                                                                                                                   <  1:178503/249‑1 (MQ=255)                                                       ccACTTTTATCAAGAAGATTATCAATGTCTTGCGAAGTAATCCGCTCCCCATCTTTACCGATAATTTCTTCAACTTTATTATTGAGTGATTTGTCGACTTTACCTCGCTGTAACTCAAATTGTCTCTTAAGTCGTGCACGATCCTTTTCAGGTGCGTATTCAAGAAGTTTTCTTTGATACTCATTTAACCCCGCCGACGAATTAGGTTTATTAAACCATCCTTTAAATGTTACTGCTGTTCTATTATTTGt                                                                                                                                              <  1:881081/251‑1 (MQ=255)                                                          cttttATCAAGAAGATTATCAATGTCTTGCGAAGTAATCCGCTCCCCATCTTTACCGATAATTTCTTCAACTTTATTATTGAGTGATTTGTCGACTTTACCTCGCTGTAACTCAAATTGTCTCTTAAGTCGTGCACGATCCTTTTCAGGTGCGTATTCAAGAAGTTTTCTTTGATACTCATTTaacc                                                                                                                                                                                                           >  1:148681/4‑185 (MQ=255)                                                          cttttATCAAGAAGATTATCAATGTCTTGCGAAGTAATCCGCTCCCCATCTTTACCGATAATTTCTTCAACTTTATTATTGAGTGATTTGTCGACTTTACCTCGCTGTAACTCAAATTGTCTCTTAAGTCGTGCACGATCCTTTTCAGGTGCGTATTCAAGAAGTTTTCTTTGATACTCATTTAACCCCGCCGACGAATTAGGTTTATTAAACCATCCTTTAAATGTTACTGCTGTTCTATTATTTGtttt                                                                                                                                           >  1:466711/4‑251 (MQ=255)                                                              tATCAAGAAGATTATCAATGTCTTGCGAAGTAATCCGCTCCCCATCTTTACCGATAATTTCTTCAACTTTATTATTGAGTGATTTGTCGACTTTACCTCGCTGTAACTCAAATTGTCTCTTAAGTCGTGCACGATCCTTTTCAGGTGCGTATTCAAGAAGTTTTCTTTGATACTCATTTAACCCCGCCGACGAATTAGGTTTATTAAACCATCCTTTAAATGTTACTGCTGTTCTATTATTTGTTTTCAAt                                                                                                                                       <  1:419986/251‑1 (MQ=255)                                                                 caagaaGATTATCAATGTCTTGCGAAGTAATCCGCTCCCCATCTTTACCGATAATTTCTTCAACTTTATTATTGAGTGATTTGTCGACTTTACCTCGCTGTAACTCAAATTGTCTCTTAAGTCGTGCACGATCCTTTTCAGGTGCGTATTCAAGAAGTTTTCTTTGATACTCATTTAACCCCGCCGACGAATTAGGTTTATTAAACCATCCTTTAAATGTTACTGCTGTTCTATTATTTGTTTTCAAtagt                                                                                                                                    <  1:149986/247‑1 (MQ=255)                                                                    gaaGATTATCAATGTCTTGCGAAGTAATCCGCTCCCCATCTTTACCGATAATTTCTTCAACTTTATTATTGAGTGATTTGTCGACTTTACCTCGCTGTAACTCAAATTGTCTCTTAAGTCGTGCACGATCCTTTTCAGGTGCGTATTCAAGAAGTTTTCTTTGATACTCATTTAACCCCGCCGACGAATTAGGTTTATTAAACCATCCTTTAAATGTTACTGCTGTTCtat                                                                                                                                                     >  1:660778/2‑231 (MQ=255)                                                                        aTTATCAATGTCTTGCGAAGTAATCCGCTCCCCATCTTTACCGATAATTTCTTCAACTTTATTATTGAGTGATTTGTCGACTTTACCTCGCTGTAACTCAAATTGTCTCTTAAGTCGTGCACGATCCTTTTCAGGTGCGTATTCAAGAAGTTTTCTTTGATACTCATTTAACCCCGCCGACGAATTAGGTTTATTAAACCATCCTTTAAATGTTACTGCTGTTCTATTATTTGTTTTCAATAGTAGATc                                                                                                                               <  1:886108/249‑1 (MQ=255)                                                                         ttATCAATGTCTTGCGAAGTAATCCGCTCCCCATCTTTACCGATAATTTCTTCAACTTTATTATTGAGTGATTTGTCGACTTTACCTCGCTGTAACTCAAATTGTCTCTTAAGTCGTGCACGATCCTTTTCAGGTGCGTATTCAAGAAGTTTTCTTTGATACTCATTTAACCCCGCCGACGAATTAGGt                                                                                                                                                                                          <  1:771750/189‑1 (MQ=255)                                                                           aTCAATGTCTTGCGAAGTAATCCGCTCCCCATCTTTACCGATAATTTCTTCAACTTTATTATTGAGTGATTTGTCGACTTTACCTCGCTGTAACTCAAATTGTCTCTTAAGTCGTGCACGATCCTTTTCAGGTGCGTATTCAAGAAGTTTTCTTTGATACTCATTTAACCCCGCCGACGAATTAGGTTTATTAAACCATCCTTTAAATGTTACTGCTGTTCTATTATTTGTTTTCAAtag                                                                                                                                     <  1:626845/240‑1 (MQ=255)                                                                                   cTTGCGAAGTAATCCGCTCCCCATCTTTACCGATAATTTCTTCAACTTTATTATTGAGTGATTTGTCGACTTTACCTCGCTGTAACTCAAATTGTCTCTTAAGTCGTGCACGATCCTTTTCAGGTGCGTATTCAAGAAGTTTTCTTTGATACTCATTTAACCCCGCCGACGAATTAGGTTTATTAAACCATCCTTTAAATGTTACTGCTGTTCtatta                                                                                                                                                   >  1:873669/1‑218 (MQ=255)                                                                                               tCCGCTCCCCATCTTTACCGATAATTTCTTCAACTTTATTATTGAGTGATTTGTCGACTTTACCTCGCTGTAACTCAAATTGTCTCTTAAGTCGTGCACGATCCTTTTCAGGTGCGTATTCAAGAAGTTTTCTTTGATACTCATTTAACCCCGCCGACGAATTAGGTTTATTAAACCATCCTTTAAATGTTACTGCTGTTCTATTATTTGTTTTCAATAGTAGATCATTGCCGTTACGTTCAAATGacacc                                                                                                      <  1:228073/251‑2 (MQ=255)                                                                                                   cTCCCCATCTTTACCGATAATTTCTTCAACTTTATTATTGAGTGATTTGTCGACTTTACCTCGCTGTAACTCAAATTGTCTCTTAAGTCGTGCACGATCCTTTTCAGGTGCGTATTCAAGAAGTTTTCTTTGATACTCATTTAACCCCGCCGACGAATTAGGTTTATTAAACCATCCTTTAAAt                                                                                                                                                                     <  1:644609/184‑1 (MQ=255)                                                                                                   cTCCCCATCTTTACCGATAATTTCTTCAACTTTATTATTGAGTGATTTGTCGACTTTACCTCGCTGTAACTCAAATTGTCTCTTAAGTCGTGCACGATCCTTTTCAGGTGCGTATTCAAGAAGTTTTCTTTGATACTCATTTAACCCCGCCGACGAATTAGGTTTATTAAACCATCCTTTAAATGTTACTGCTGTTCTATTATTTGTTTTCAATAGTAGATCATTGcc                                                                                                                         >  1:611789/1‑228 (MQ=255)                                                                                                   cTCCCCATCTTTACCGATAATTTCTTCAACTTTATTATTGAGTGATTTGTCGACTTTACCTCGCTGTAACTCAAATTGTCTCTTAAGTCGTGCACGATCCTTTTCAGGTGCGTATTCAAGAAGTTTTCTTTGATACTCATTTAACCCCGCCGACGAATTAGGTTTATTAAACCATCCTTTAAATGTTACTGCTGTTCTATTATTTGTTTTCAATAGTAGATCATTGCCGTTACGTTCAAATGACACCTCtt                                                                                                  >  1:253802/1‑251 (MQ=255)                                                                                                   cTCCCCATCTTTACCGATAATTTCTTCAACTTTATTATTGAGTGATTTGTCGACTTTACCTCGCTGTAACTCAAATTGTCTCTTAAGTCGTGCACGATCCTTTTCAGGTGCGTATTCAAGAAGTTTTCTTTGATACTCATTTAACCCCGCCGACGAATTAGGTTTATTAAACCATCCTTTAAATGTTACTGCTGTTCTATTATTTGTTTTCAATAGTAGATCATTGCCGTTACGTTCAAATGACACCTCtt                                                                                                  >  1:881486/1‑251 (MQ=255)                                                                                                        cATCTTTACCGATAATTTCTTCAACTTTATTATTGAGTGATTTGTCGACTTTACCTCGCTGTAACTCAAATTGTCTCTTAAGTCGTGCACGATCCTTTTCAGGTGCGTATTCAAGAAGTTTTCTTTGATACTCATTTAACCCCGCCGACGAATTAGGTTTATTAAACCATCCTTTAAATGTTACTGCTGTTCTATTATTTGTTTTCAATAGTAGATCATTGc                                                                                                                          <  1:580957/222‑1 (MQ=255)                                                                                                        cATCTTTACCGATAATTTCTTCAACTTTATTATTGAGTGATTTGTCGACTTTACCTCGCTGTAACTCAAATTGTCTCTTAAGTCGTGCACGATCCTTTTCAGGTGCGTATTCAAGAAGTTTTCTTTGATACTCATTTAACCCCGCCGACGAATTAGGTTTATTAAACCATCCTTTAAATGTTACTGCTGTTCTATTATTTGTTTTCAATAGTAGATCATTGCCGTTACGTTCAAATGACACCTCTTTgagg                                                                                             >  1:411760/1‑250 (MQ=255)                                                                                                                        ttcttcAACTTTATTATTGAGTGATTTGTCGACTTTACCTCGCTGTAACTCAAATTGTCTCTTAAGTCGTGCACGATCCTTTTCAGGTGCGTATTCAAGAAGTTTTCTTTGATACTCATTTaacc                                                                                                                                                                                                           <  1:870252/125‑3 (MQ=255)                                                                                                                        ttcttcAACTTTATTATTGAGTGATTTGTCGACTTTACCTCGCTGTAACTCAAATTGTCTCTTAAGTCGTGCACGATCCTTTTCAGGTGCGTATTCAAGAAGTTTTCTTTGATACTCATTTAACCCCGCCGACGAATTAGGTTTATTAAACCATCCTTTAAATGTTACTGCTGTTCTATTATTTGTTTTCAATAGTAGATCATTGCCGTTACGTTCAAATGACACCTCTTTGAGGTTGATATTTGCTAATg                                                                             >  1:503510/1‑251 (MQ=255)                                                                                                                               aCTTTATTATTGAGTGATTTGTCGACTTTACCTCGCTGTAACTCAAATTGTCTCTTAAGTCGTGCACGATCCTTTTCAGGTGCGTATTCAAGAAGTTTTCTTTGATACTCATTTAACCCCGCCGACGAATTAGGTTTATTAAACCATCCTTTAAATGTTACTGCTGTTCTATTATTTGTTTTCAATAGTAGATCATTGc                                                                                                                          >  1:262720/1‑199 (MQ=255)                                                                                                                               aCTTTATTATTGAGTGATTTGTCGACTTTACCTCGCTGTAACTCAAATTGTCTCTTAAGTCGTGCACGATCCTTTTCAGGTGCGTATTCAAGAAGTTTTCTTTGATACTCATTTAACCCCGCCGACGAATTAGGTTTATTAAACCATCCTTTAAATGTTACTGCTGTTCTATTATTTGTTTTCAATAGTAGATCATTGCCGTTACGTTCAAATGACACCTCTTTgaggt                                                                                            >  1:862789/1‑227 (MQ=255)                                                                                                                               aCTTTATTATTGAGTGATTTGTCGACTTTACCTCGCTGTAACTCAAATTGTCTCTTAAGTCGTGCACGATCCTTTTCAGGTGCGTATTCAAGAAGTTTTCTTTGATACTCATTTAACCCCGCCGACGAATTAGGTTTATTAAACCATCCTTTAAATGTTACTGCTGTTCTATTATTTGTTTTCAATAGTAGATCATTGCCGTTACGTTCAAATGACACCTCTTTGAGGTTGATATTTGCTAATGATAAttt                                                                      <  1:498014/251‑1 (MQ=255)                                                                                                                               aCTTTATTATTGAGTGATTTGTCGACTTTACCTCGCTGTAACTCAAATTGTCTCTTAAGTCGTGCACGATCCTTTTCAGGTGCGTATTCAAGAAGTTTTCTTTGATACTCATTTAACCCCGCCGACGAATTAGGTTTATTAAACCATCCTTTAAATGTTACTGCTGTTCTATTATTTGTTTTCAATAGTAGATCATTGCCGTTACGTTCAAATGACACCTCTTTGAGGTTGATATTTGCTAATGATAAttt                                                                      >  1:85730/1‑251 (MQ=255)                                                                                                                                cTTTATTATTGAGTGATTTGTCGACTTTACCTCGCTGTAACTCAAATTGTCTCTTAAGTCGTGCACGATCCTTTTCAGGTGCGTATTCAAGAAGTTTTCTTTGATACTCATTTAACCCCGCCGACGAATTAGGTTTatt                                                                                                                                                                                     >  1:112210/1‑137 (MQ=255)                                                                                                                                cTTTATTATTGAGTGATTTGTCGACTTTACCTCGCTGTAACTCAAATTGTCTCTTAAGTCGTGCACGATCCTTTTCAGGTGCGTATTCAAGAAGTTTTCTTTGATACTCATTTAACCCCGCCGACGAATTAGGTTTATTAAACCATCCTTTAAATg                                                                                                                                                                    >  1:361808/1‑156 (MQ=255)                                                                                                                                  ttattaTTGAGTGATTTGTCGACTTTACCTCGCTGTAACTCAAATTGTCTCTTAAGTCGTGCACGATCCTTTTCAGGTGCGTATTCAAGAAGTTTTCTTTGATACTCATTTaac                                                                                                                                                                                                            >  1:527746/1‑113 (MQ=255)                                                                                                                                  ttattaTTGAGTGATTTGTCGACTTTACCCCGCTGTAACTCAAATTGTCTCTTAAGTCGTGCACGATCCTTTTCAGGTGCGTATTCAAGAAGTTTTCTTTGATACTCATTTAACCCCGCCGACGAATTAGGTTTATTAAACCATCCTTTAAATGTTACTGCTGTTCTATTATTTGTTTTCAATAGTAGATCATTGCCGTTACGTTCAAATGACACCTCTTTGAGGTTGATATTTGCTa                                                                                <  1:901593/238‑1 (MQ=255)                                                                                                                                    attattGAGTGATTTGTCGACTTTACCTCGCTGTAACTCAAATTGTCTCTTAAGTCGTGCACGATCCTTTTCAGGTGCGTATTCAAGAAGTTTTCTTTGATACTCATTTAACCCCGCCGACGAATTAGGTTTATTAAACCATCCTTTAAATGTTACTGCTGTTCTATTATTTg                                                                                                                                               <  1:839441/173‑1 (MQ=255)                                                                                                                                    attattGAGTGATTTGTCGACTTTACCTCGCTGTAACTCAAATTGTCTCTTAAGTCGTGCACGATCCTTTTCAGGTGCGTATTCAAGAAGTTTTCTTTGATACTCATTTAACCCCGCCGACGAATTAGGTTTATTAAACCATCCTTTAAATGTTACTGCTGTTCTATTATTTGTTTTCAATAGTAGATCATTGCCGTTACGTTCAAATGACACCTCTTTGAGGTTGATATTTGCTAATGATAAtttatctt                                                                 >  1:478427/1‑251 (MQ=255)                                                                                                                                              gATTTGTCGACTTTACCTCGCTGTAACTCAAATTGTCTCTTAAGTCGTGCACGATCCTTTTCAGGTGCGTATTCAAGAAGTTTTCTTTGATACTCATTTAACCCCGCCGACGAATTAGGt                                                                                                                                                                                          >  1:573390/1‑120 (MQ=255)                                                                                                                                                tttGTCGACTTTACCTCGCTGTAACTCAAATTGTCTCTTAAGTCGTGCACGATCCTTTTCAGGTGCGTATTCAAGAAGTTTTCTTTGATACTCATTTAACCCCGCCGACGAATTAGGTTTATTAAACCATCCTTTAAATGTTACTGCTGTTCTATTATTTGTTTTCAATAGTAGATCATTGCCGTTACGTTCAAATGACACCTCTTTGAGGTTGATATTTGCTAATGATAATTTATCTTTATCACCGCTAt                                                     >  1:723430/1‑251 (MQ=255)                                                                                                                                                  tGTCGACTTTACCTCGCTGTAACTCAAATTGTCTCTTAAGTCGTGCACGATCCTTTTCAGGTGCGTATTCAAGAAGTTTTCTTTGATACTCATTTAACCCCGCCGACGAATTAGGTTTATTAAACCATCCTTTAAATGTTACTGCTGTTCTATTATTTGTTTTCAATAGTAGATCATTGCCGTTACGTTCAAATGACACCTCTTTGAGGTTGATATTTGCTAATGATAATTTATCTTTATCACCGCTATGt                                                   <  1:620549/251‑1 (MQ=255)                                                                                                                                                  accgatcgttACCTCGCTGTAACTCAAATTGTCTCTTAAGTCGTGCACGATCCTTTTCAGGTGCGTATTCAAGAAGTTTTCTTTGATACTCATTTAACCCCGCCGACGAATTAGGTTTATTAAACCATCCTTTAAATGTTACTGCTGTTCTATTATTTGTTTTCAATAGTAGATCATTGCCGTTACGTTCAAATGACACCTCTTTGAGGTTGATATTTGCTAATGATAATTTATCTTTATCACCGCTATGt                                                   <  1:130494/243‑1 (MQ=255)                                                                                                                                                    tCGACTTTACCTCGCTGTAACTCAAATTGTCACTTAAGTCGTGCACGATCCTTTTCAGGTGCGTATTCAAGAAGTTTTCTTTGATACTCATTTAACCCCGCCGACGAATTAGGTTTATTAAACCATCCTTTAAATGTTACTGCTGTTCTATTATTTGTTTTCAATAGTAGATCATTGCCGTTACGTTCAAATGACACCTCTTTGAGGTTGATATTTGCTAATGATAATTTATCTTTATCACCGCTATGTTc                                                 <  1:262955/251‑1 (MQ=255)                                                                                                                                                       aCTTTACCTCGCTGTAACTCAAATTGTCTCTTAAGTCGTGCACGATCCTTTTCAGGTGCGTATTCAAGAAGTTTTCTTTGATACTCATTTAACCCCGCCGACGAATTAGGTTTATTAAACCATCCTTTAAATGTTACTGCTGTTc                                                                                                                                                        <  1:813077/145‑1 (MQ=255)                                                                                                                                                       aCTTTACCTCGCTGTAACTCAAATTGTCTCTTAAGTCGTGCACGATCCTTTTCAGGTGCGTATTCAAGAAGTTTTCTTTGATACTCATTTAACCCCGCCGACGAATTAGGTTTATTAAACCATCCTTTAAATGTTACTGCTGTTCTATTATTTGTTTTCAATAGTAGATCATTGCCGTTACGTTCAAATGACACCTCTTTGAGGTTGATATTTGCTAATGATAATTTATCTTTATCACCGCTATGTTCCGt                                              >  1:513353/1‑251 (MQ=255)                                                                                                                                                         tttACCTCGCTGTAACTCAAATTGTCTCTTAAGTCGTGCACGATCCTTTTCAGGTGCGTATTCAAGAAGTTTTCTTTGATACTCATTTAACCCCGCCGACGAATTAGGTTTATTAAACCATCCTTTAAATGTTACTGCTGTTCTATTATTTGTTTTCAATAGTAGATCATTGCCGTTACGTTCAAATGACACCTCTTTGAGGTTGATATTTGCTAATGATAATTTATCTTTATCACCGCTATGTTCCGTaa                                            <  1:734771/251‑1 (MQ=255)                                                                                                                                                          ttACCTCGCTGTAACTCAAATTGTCTCTTAAGTCGTGCACGATCCTTTTCAGGTGCGTATTCAAGAAGTTTTCTTTGATACTCATTTAACCCCGCCGACGAATTAGGTTTATTAAACCATCCTTTAAATGTTACTGCTGTTCTATTATTTGTTTTCAATAGTAGATCATTGCCGTTACGTTCAAATGACACCTCTTTGAGGTTGATATTTGCTAATGATAAtttatc                                                                   >  1:31104/1‑227 (MQ=255)                                                                                                                                                          ttACCTCGCTGTAACTCAAATTGTCTCTTAAGTCGTGCACGATCCTTTTCAGGTGCGTATTCAAGAAGTTTTCTTTGATACTCATTTAACCCCGCCGACGAATTAGGTTTATTAAACCATCCTTTAAATGTTACTGCTGTTCTATTATTTGTTTTCAATAGTAGATCATTGCCGTTACGTTCAAATGACACCTCTTTGAGGTTGATATTTGCTAATGATAATTTATCTTTATCACCGCTATGTTCCGTAAt                                           <  1:799419/251‑1 (MQ=255)                                                                                                                                                            aCCTCGCTGTAACTCAAATTGTCTCTTAAGTCGTTCACGATCCTTTTCAGGTGCGTATTCAAGAAGTTTTCTTTGATACTCATTTAACCCCGCCGACGAATTAGGTTTATTAAACCATCCTTTAAATGTTACTGCTGTTCTATTATTTGTTTTCAATAGTAGATCATTGCCGTTACGTTCAAATGACACCTCTTTGAGGTTGATATTTGCTAATGATAATTTATCTTTATCACCGCTATGTTCCGTAATAg                                         >  1:644816/1‑251 (MQ=255)                                                                                                                                                            aCCTCGCTGTAACTCAAATTGTCTCTTAAGTCGTGCACGATCCTTTTCAGGTGCGTATTCAAGAAGTTTTCTTTGATACTCATTTAACCCCGCCGACGAATTAGGTTTATTAAACCATCCTTTAAATGTTACTGCTGTTCTATTATTTGTTTTCAATAGTAGATCATTGCCGTTACGTTCAAATGACACCTCTTTGAGGTTGATATTTGCTAATGATAATTTATCTTTATCACCGCTATGTTCCGTAATAg                                         <  1:355687/251‑1 (MQ=255)                                                                                                                                                                    gTAACTCAAATTGTCTCTTAAGTCGTGCACGATCCTTTTCAGGTGCGTATTCAAGAAGTTTTCTTTGATACTCATTTAACCCCGCCGACGAATTAGGTTTATTAAACCATCCTTTAAATGTTACTGCTGTTCTATTATTTGTTTTCAATAGTAGATCATTGCCGTTACGTTCAAATGACACCTCTTTGAGGTTGATATTTGCTAATGATAATTTATCTTCATCACCGCTATGTTc                                                 <  1:437/235‑1 (MQ=255)                                                                                                                                                                      aaCTCAAATTGTCTCTTAAGTCGTGCACGATCCTTTTCAGGTGCGTATTCAAGAAGTTTTCTTTGATACTCATTTAACCCCGCCGACGAATTAGGTTTATTAAACCATCCTTTAAATGTTACTGCTGTTCTATTATTTGTTTTCAATAGTAGATCATTGCCGTTACGTTCAAATGACACCTCTTTGAGGTTGATATTTGCTAATGATAATTTATCTTTATCACCGCTATGTTCCGTAATAGTgtggtggcc                               <  1:543756/251‑4 (MQ=255)                                                                                                                                                                       aCTCAAATTGTCTCTTAAGTCGTGCACGATCCTTTTCAGGTGCGTATTCAAGAAGTTTTCTTTGATACTCATTTAACCCCGCCGACGAATTAGGTTTATTAAACCATCCTTTAAATGTTACTGCTGTTCTATTATTTGTTTTCAATAGTAGATCATTGCCGTTACGTTCAAATGACACCTCTTTgag                                                                                              <  1:528924/187‑1 (MQ=255)                                                                                                                                                                                  ctctTAAGTCGTGCACGATCCTTTTCAGGTGCGTATTCAAGAAGTTTTCTTTGATACTCATTTAACCCCGCCGACGAATTAGGTTTATTAAACCATcc                                                                                                                                                                            <  1:305918/98‑1 (MQ=255)                                                                                                                                                                                  ctctTAAGTCGTGCACGATCCTTTTCAGGTGCGTATTCAAGAAGTTTTCTTTGATACTCATTTAACCCCGCCGACGAATTAGGTTTATTAAACCATCCTTTAAATGTTACTGCTGTTCTATTATTTGTTTTCAATAGTAGATCATTGCCGTTACGTTCAAATg                                                                                                           >  1:177011/1‑163 (MQ=255)                                                                                                                                                                                    ctTAAGTCGTGCACGATCCTTTTCAGGTGCGTATTCAAGAAGTTTTCTTTGATACTCATTTAACCCCGCCGACGAATTAGGTTTATTAAACCATCCTTTAAATGTTACTGCTGTTCTATTATTTGTTTTCAATAGTAGATCATTGCCGTTACGTTCAAATGACACCTCTTTGAGGTTGATATTTGCTAATGATAATTTATCTTTATCACCGCTATGTTCCGTAATAGTGTGGTGGCCATATTCCTTACGGt                 <  1:488531/251‑1 (MQ=255)                                                                                                                                                                                                   aTCCTTTTCAGGTGCGTATTCAAGAAGTTTTCTTTGATACTCATTTAACCCCGCCGACGAATTAGGTTTATTAAACCATCCTTTAAATGTTACTGCTGTTCTATTAttt                                                                                                                                                >  1:429571/1‑109 (MQ=255)                                                                                                                                                                                                   aTCCTTTTCAGGTGCGTATTCAAGAAGTTTTCTTTGATACTCATTTAACCCCGCCGACGAATTAGGTTTATTAAACCATCCTTTAAATGTTACTGCTGTTCTATTATTTGTTTTCAATAGTAGATCATTGCCGTTACGTTCAAATGACACCTCTTTGAGGTTGATATTTGCTAATGATAAttt                                                                      <  1:595542/183‑1 (MQ=255)                                                                                                                                                                                                   aTCCTTTTCAGGTGCGTATTCAAGAAGTTTTCTTTGATACTCATTTAACCCCGCCGACGAATTAGGTTTATTAAACCATCCTTTAAATGTTACTGCTGTTCTATTATTTGTTTTCAATAGTAGATCATTGCCGTTACGTTCAAATGACACCTCTTTGAGGTTGATATTTGCTAATGATAATTTATCTTTATCACCGCTATGTTCCGTAATAg                                         >  1:821658/1‑212 (MQ=255)                                                                                                                                                                                                   aTCCTTTTCAGGTGCGTATTCAAGAAGTTTTCTTTGATACTCATTTAACCCCGCCGACGAATTAGGTTTATTAAACCATCCTTTAAATGTTACTGCTGTTCTATTATTTGTTTTCAATAGTAGATCATTGCCGTTACGTTCAAATGACACCTCTTTGAGGTTGATATTTGCTAATGATAATTTATCTTTATCACCGCTATGTTCCGTAATAGTGTGGTGGCCATATTCCTTACGGTAAACATAAATATCAt  >  1:287418/1‑251 (MQ=255)                                                                                                                                                                                                      cTTTTCAGGTGCGTATTCAAGAAGTTTTCTTTGATACTCATTTAACCCCGCCGACGAATTAGGTTTATTAAACCATCCTTTAAATGTTACTGCTGTTCTATTATTTGTTTTCAATAGTAGATCATTGCCGTTACGTTCAAAt                                                                                                            <  1:616103/142‑1 (MQ=255)                                                                                                                                                                                                      cTTTTCAGGTGCGTATTCAAGAAGTTTTCTTTGATACTCATTTAACCCCGCCGACGAATTAGGTTTATTAAACCATCCTTTAAATGTTACTGCTGTTCTATTATTTGTTTTCAATAGTAGATCATTGCCGTTACGTTCAAATGACACCTCTTTGAGGTTGATATTTGCTAATGATAATTTATCTTTATCACCGCTATGTTCCGTAATAGTGTGGTGGCCATATTc                         <  1:733854/225‑1 (MQ=255)                                                                                                                                                                                                                                               |                                                                                                                                                                                                               CCTTTATTCTTAATAAGTCCGGCAAGCTCTTGAGGTGAAATTGTCTTTTTGTTCCCACTCTTATCAAAAAGATTATCAATGTCTTGCGAAGTAATCCGCTCCCCATCTTTACCGATAATTTCTTCAACTTTATTATTGAGTGATTTGTCGACTTTACCTCGCTGTAACTCAAATTGTCTCTTAAGTCGTGCACGATCCTTTTCAGGTGCGTATTCAAGAAGTTTTCTTTGATACTCATCTAATCCTGCCGATGAATTAGGTTTACTAAACCATCCTTTAAATGTTACTGCTGTTCTATTATTTGTTTTCAATAGTAGATCATTGCCGTTACGCTCAAATGACACATCTTTGAGATTGATATTTGCTAATGATAATTTATCTTTATCACCGCTATGTTCCGTAATAGTGTGGTGCCCATATTCTTTACGGTAAACATAAATATCAT  >  1/1979155‑1979599

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑