Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,978,701	C→T	V64I (GTT→ATT)	unknown ←	RTX toxin transporter, ATP‑binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,978,701	0	C	T	100.0%	128.2 / NA	46	V64I (GTT→ATT)	unknown	RTX toxin transporter, ATP‑binding protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (18/28);  total (18/28)

CAGGAATAAACCAGGTAAAGTCGAATTTTGCTAAATTTCCTACAATAGATGCGCGTGATGCAATTAAAATCACCTTGCTTTGATAACGTTCTTCAAATTCATTTTTGTCTAAAACAATAGGATTTTTTTGAATTAGATCAAAAATGAGGTAACGATCGGTTTCTTGATCTATTTTTAATAAAATAAAGTGTTCACCATCATCACGCCAAGCCAATGCCGGTAAATGTAAAAAAGGCAATCTGTCAACTGTTTTGTTCGCAGTTCTTACCTTTAATCCAAGTGATTTTGCTGCAAGCAACCATTTAGTTTGGTTCAGCCCATGCCCATCTATATCAAATTTATGTTTTATTTCTTCCGGGTTAATTGAAATATTATGATATTGAGCCAGTACTTCCAATGCCTGAAGGGCAAGATTAGTATTTTTCTGTGAGTCCATTACATATCCTGAAAAACAGTGATTCCGCACACACAAATATGC  >  1/1978455‑1978932                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                        cAGGAATAAACCAGGTAAAATCGAATTTTGCTAAATTTCCTACAATAGATGCGCGTGACGCAACTAAAATCACCTTGCTTTGATAACGTTCTTCAAATTCATTTTTGTCTAAAACAATAGGATTTTTTTGCATTAGATCAAAAATGAGGTAACGATCGGTTTCTTGATCTATTTTCAATAAAATAAAGTGGTCACCATCATCACGCCAAGCCAATACCGGTAAATGTAAAAAAGGTAATCTATCAATTGt                                                                                                                                                                                                                                      <  1:570325/250‑1 (MQ=255)   ggAATAAACCAGGTAAAATCGAATTTTGCTAAATTTCCTACAATAGATGCGCGTGACGCAACTAAAATCACCTTGCTTTGATAACGTTCTTCAAATTCATTTTTGTCTAAAACAATAGGATTTTTTTGCATTAGATCAAAAATGAGGTAACGATCGGTTTCTTGATCTATTTTCAATAAAATAAAGTGGTCACCATCATCACGCCAAGCCAATACCGGTAAATGTAAAAAAGGTAATCTATCAATTGtttt                                                                                                                                                                                                                                   >  1:541494/1‑251 (MQ=255)                   aatCGAATTTTGCTAAATTTCCTACAATAGATGCGCGTGACGCAACTAAAATCACCTTGCTTTGATAACGTTCTTCAAATTCATTTTTGTCTAAAACAATAGGATTTTTTTGCATTAGATCAAAAATGAGGTAACGATCGGTTTCTTGATCTATTTTCAATAAAATAAAGTGGTCACCATCATCACGCCAAGCCAATACCGGTAAATGTAAAAAAGGTAATCTATCAATTGTTTTGTTCGTAGTTCTTa                                                                                                                                                                                                                     >  1:636608/3‑249 (MQ=255)                                           cAATAGATGCGCGTGACGCAACTAAAATCACCTTGCTTTGATAACGTTCTTCAAATTCATTTTTGTCTAAAACAATAGGATTTTTTTGCATTAGATCAAAAATGAGGTAACGATCGGTTTCTTGATCTATTTTCAATAAAATAAAGTGGTCACCATCATCACGCCAAGCCAATACCGGTAAATGTAAAAAAGGTAATCTATCAATTGTTTTGTTCGTAGTTCTTACTTTTAATc                                                                                                                                                                                                            >  1:19746/1‑234 (MQ=255)                                           cAATAGATGCGCGTGACGCAACTAAAATCACCTTGCTTTGATAACGTTCTTCAAATTCATTTTTGTCTAAAACAATAGGATTTTTTTGCATTAGATCAAAAATGAGGTAACGATCGGTTTCTTGATCTATTTTCAATAAAATAAAGTGGTCACCATCATCACGCCAAGCCAATACCGGTAAATGTAAAAAAGGTAATCTATCAATTGTTTTGTTCGTAGTTCTTACTTTTAATCCAAGTGATCTtgctg                                                                                                                                                                                             <  1:339897/249‑1 (MQ=255)                                                                    aaTCACCTTGCTTTGATAACGTTCTTCAAATTCATTTTTGTCTAAAACAATAGGATTTTTTTGCATTAGATCAAAAATGAGGTAACGATCGGTTTCTTGATCTATTTTCAATAAAATAAAGTGGTCACCATCATCACGCCAAGCCAATACCGGTAAATGTAAAAAAGGTAATCTATCAATTGTTTTGTTCGTAGTTCTTACTTTTAATCCAAGTGATCTTGCTGCAAGCAACCATTTAGTTTGGTTCAgcc                                                                                                                                                                  <  1:183974/251‑1 (MQ=255)                                                                     aTCACCTTGCTTTGATAACGTTCTTCAAATTCATTTTTGTCTAAAACAATAGGATTTTTTTGCATTAGATCAAAAATGAGGTAACGATCGGTTTCTTGATCTATTTTCAATAAAATAAAGTGGTCACCATCATCACGCCAAGCCAATACCGGTAAATGTAAAAAAGGTAATCTATCAATTGTTTTGTTCGTAGTTCTTACTTTTAATCCAAGTGATCTTGCTGCAAGCAACCATTTAGTTTGGTTCAgccc                                                                                                                                                                 <  1:622500/251‑1 (MQ=255)                                                                          cTTGCTTTGATAACGTTCTTCAAATTCATTTTTGTCTAAAACAATAGGATTTTTTTGCATTAGATCAAAAATGAGGTAACGATCGGTTTCTTGATCTATTTTCAATAAAATAAAGTGGTCACCATCATCACGCCAAGCCAATACCGGTAAATGTAAAAAAGGTAATCTATCAATTGTTTTGTTCGTAGTTCTTACTTTTAATCCAAGTGATCTTGCTGCAAGCAACCATTTAGTTTGGTTCAgcccatgcc                                                                                                                                                            <  1:217913/251‑1 (MQ=255)                                                                                           cttcAAATTCA‑TTTTGTCTAAAACAATAGGATTTTTTTGCATTAGATCAAAAATGAGGTAACGATCGGTTTCTTGATCTATTTTCAATAAAATAAAGTGGTCACCATCATCACGCCAAGCCAATACCGGTAAATGTAAAAAAGGTAATCTATCAATTGTTTTGTTCGTAGTTCTTACTTTTAATCCAAGTGATCTTGCTGCAAGCAACCATTTAGTTTGGTTCAGCCCATGCCCATCTATATCAAATTTAt                                                                                                                                          >  1:611760/1‑251 (MQ=255)                                                                                                    cATTTTTGTCTAAAACAATAGGATTTTTTTGCATTAGATCAAAAATGAGGTAACGATCGGTTTCTTGATCTATTTTCAATAAAATAAAGTGGTCACCATCATCACGCCAAGCCAATACCGGTAAATGTAAAAAAGGTAATCTATCAATTGTTTTGTTCGTAGTTCTTACTTTTAATCCAAGTGATCTTGCTGCAAGCAACCATTTAGTTTGGTTCAGCCCATGCCCATCTATATCAAATTTATGTTTTAtt                                                                                                                                  >  1:875279/1‑251 (MQ=255)                                                                                                           gTCTAAAACAATAGGATTTTTTTGCATTAGATCAAAAATGAGGTAACGATCGGTTTCTTGATCTATTTTCAATAAAATAAAGTGGTCACCATCATCACGCCAAGCCAATACCGGTAAATGTAAAAAAGGTAATCTATCAATTGTTTTGTTCGTAGTTCTTAct                                                                                                                                                                                                                   <  1:400639/163‑2 (MQ=255)                                                                                                           gTCTAAAACAATAGGATTTTTTTGCATTAGATCAAAAATGAGGTAACGATCGGTTTCTTGATCTATTTTCAATAAAATAAAGTGGTCACCATCATCACGCCAAGCCAATACCGGTAAATGTAAAAAAGGTAATCTATCAATTGTTTTGTTCGTAGTTCTTACTTTTAATCCAAGTGATCTTGCTGCAAGCAACCATTTAGTTTGGTTc                                                                                                                                                                      >  1:313598/1‑208 (MQ=255)                                                                                                                aaaCAATAGGATTTTTTTGCATTAGATCAAAAATGAGGTAACGATCGGTTTCTTGATCTATTTTCAATAAAATAAAGTGGTCACCATCATCACGCCAAGCCAATACCGGTAAATGTAAAAAAGGTAATCTATCAATTGTTTTGTTCGTAGTTCTTACTTTTAATCCAAGTGATCTTGCTGCAAGCAACCATTTAGTTTGGTTCAgcccatgcc                                                                                                                                                            >  1:148087/1‑213 (MQ=255)                                                                                                                   cAATAGGATTTTTTTGCATTAGATCAAAAATGAGGTAACGATCGGTTTCTTGATCTATTTTCAATAAAATAAAGTGGTCACCATCATCACGCCAAGCCAATACCGGTAAATGTAAAAAAGGTAATCTATCAATTGTTTTGTTCGTAGTTCTTACTTTTAATc                                                                                                                                                                                                            >  1:348643/1‑162 (MQ=255)                                                                                                                   cAATAGGATTTTTTTGCATTAGATCAAAAATGAGGTAACGATCGGTTTCTTGATCTATTTTCAATAAAATAAAGTGGTCACCATCATCACGCCAAGCCAATACCGGTAAATGTAAAAAAGGTAATCTATCAATTGTTTTGTTCGTAGTTCTTACTTTTAATCCAAGTGATCTTGCTGCAAGCAACCATTTAGTTTGGTTCAGCCCATGCCCATCTATATCAAATTTATGTTTTATTTCTTCAGGGTTAAtt                                                                                                                   >  1:379943/1‑251 (MQ=255)                                                                                                                    aaTAGGATTTTTTTGCATTAGATCAAAAATGAGGTAACGATCGGTTTCTTGATCTATTTTCAATAAAATAAAGTGGTCACCATCATCACGCCAAGCCAATACCGGTAAATGTAAAAAAGGTAATCTATCAATTGTTTTGTTCGTAGTTCTTACTTTTAATCCAAGTGATCTTGCTGCAAGCAACCATTTAGTTTGGTTCAGCCCATGCCCATCTATATCAAATTTATGTTTTATTTCTTCAGGGTTAATTg                                                                                                                  <  1:28126/251‑1 (MQ=255)                                                                                                                               tttGCATTAGATCAAAAATGAGGTAACGATCGGTTTCTTGATCTATTTTCAATAAAATAAAGTGGTCACCATCATCACGCCAAGCCAATACCGGTAAATGTAAAAAAGGTAATCTATCAATTGTTTTGTTCGTAGTTCTTACTTTTAATCCAAGTGATCTTGCTGCAAGCAACCATTTAGTTTGGTTCAGCCCATGCCCATCTATATCAAATTTATGTTTTATTTCTTCAGGGTTAATTGAAATATTATGa                                                                                                       <  1:456801/251‑1 (MQ=255)                                                                                                                                         aTCAAAAATGAGGTAACGATCGGTTTCTTGATCTATTTTCAATAAAATAAAGTGGTCACCATCATCACGCCAAGCCAATACCGGTAAATGTAAAAAAGGTAATCTATCAATTGTTTTGTTCGTAGTTCTTACTTTTAATCCAAGTGATCTTGCTGCAAGCAACCATTTAGTTTGGTTCAGCCCATGCCCATCTATATCAAATTTATGTTTTATTTCTTCAGGGTTAATTGAAATATTATGATATTGAGc                                                                                               <  1:899261/249‑1 (MQ=255)                                                                                                                                          tCAAAAATGAGGTAACGATCGGTTTCTTGATCTATTTTCAATAAAATAAAGTGGTCACCATCATCACGCCAAGCCAATACCGGTAAATGTAAAAAAGGTAATCTATCAATTGTTTTGTTCGTAGTTCTTACTTTTAATCCAAGTGATCTTGCTGCAAGCAACCATTTAGTTTGGTTCAGCCCATGCCCATCTatat                                                                                                                                                   <  1:843933/196‑1 (MQ=255)                                                                                                                                          tCAAAAATGAGGTAACGATCGGTTTCTTGATCTATTTTAAATAAAATAAAGTGGTCACCATCATCACGCCAAGCCAATACCGGTAAATGTAAAAAAGGTAATCTATCAATTGTTTTGTTCGTAGTTCTTACTTTTAATCCAAGTGATCTTGCTGCAAGCAACCATTTAGTTTGGTTCAGCCCATGCCCATCTATATCAAATTTATGTTTTATTTCTTCAGGGTTAAtt                                                                                                                   <  1:320068/228‑1 (MQ=255)                                                                                                                                             aaaaTGAGGTAACGATCGGTTTCTTGATCTATTTTCAATAAAATAAAGTGGTCACCATCATCACGCCAAGCCAATACCGGTAAATGTAAAAAAGGTAATCTATCAATTGTTTTGTTCGTAGTTCTTACTTTTAATCCAAGTGATCTTGCTGCAAGCAACCATTTAGTTTGGTTCAGCCCATGCCCATCTATATCAAATTTATGTTTTATTTCTTCAGGGTTAATTGAAATATTATGATATTGAGCCAGTAc                                                                                         <  1:146660/251‑1 (MQ=255)                                                                                                                                                  gAGGTAACGATCGGTTTCTTGATCTATTTTCAATAAAATAAAGTGGTCACCATCATCACGCCAAGCCAATACCGGTAAATGTAAAAAAGGTAATCTATCAATTGTTTTGTTCGTAGTTCTTACTTTTAATCCAAGTGATCTTGCTGCAAGCAACCATTTAGTTTGGTTCAgcccatgcc                                                                                                                                                            <  1:300891/179‑1 (MQ=255)                                                                                                                                                          gATCGGTTTCTTGATCTATTTTCAATAAAATAAAGTGGTCACCATCATCACGCCAAGCCAATACCGGTAAATGTAAAAAAGGTAATCTATCAATTGTTTTGTTCGTAGTTCTTACTTTTAATCCAAGTGATCTTGCTGCAAGCAACCTTTTAGTTTGGTTCAGCCCATGCCCATCTATATCAAATTTATGTTTTATTTCTTCAGGGTTAATTGAAATATTATGATATTGAGCCAGTACTTCCAATGCCTGa                                                                            >  1:566830/1‑251 (MQ=255)                                                                                                                                                               gTTTCTTGATCTATTTTCAATAAAATAAAGTGGTCACCATCATCACGCCAAGCCAATACCGGTAAATGTAAAAAAGGTAATCTATCAATTGTTTTGTTCGTAGTTCTTACTTTTAATCCAAGTGATCTTGCTGCAAGCAACCATTTAGTTTGGTTc                                                                                                                                                                      <  1:240188/156‑1 (MQ=255)                                                                                                                                                               gTTTCTTGATCTATTTTCAATAAAATAAAGTGGTCACCATCATCACGCCAAGCCAATACCGGTAAATGTAAAAAAGGTAATCTATCAATTGTTTTGTTCGTAGTTCTTACTTTTAATCCAAGTGATCTTGCTGCAAGCAACCATTTAGTTTGGTTCAGCCCATGCCCATc                                                                                                                                                        <  1:889672/170‑1 (MQ=255)                                                                                                                                                                      gATCTATTTTCAATAAAATAAAGTGGTCACCATCATCACGCCAAGCCAATACCGGTAAATGTAAAAAAGGTAATCTATCAATTGTTTTGTTCGTAGTTCTTACTTTTAATCCAAGTGATCTTGCTGCAAGCAACCATTTAGTTTGGTTCAGCCCATGCCCATc                                                                                                                                                        <  1:889977/163‑1 (MQ=255)                                                                                                                                                                      gATCTATTTTCAATAAAATAAAGTGGTCACCATCATCACGCCAAGCCAATACCGGTAAATGTAAAAAAGGTAATCTATCAATTGTTTTGTTCGTAGTTCTTACTTTTAATCCAAGTGATCTTGCTGCAAGCAACCATTTAGTTTGGTTCAGCCCATGCCCATCta                                                                                                                                                      >  1:799754/1‑165 (MQ=255)                                                                                                                                                                                 aataaaataaAGTGGTCACCATCATCACGCCAAGCCAATACCGGTAAATGTAAAAAAGGTAATCTATCAATTGTTTTGTTCGTAGTTCTTACTTTTAATCCAAGTGATCTTGCTGCAAGCAACCATTTAGTTTGGTTCAGCCCATGCCCATCTATATCAAATTTATGTTTTATTTCTTCAGGGTTAATTGAAATATTATGATATTGAGCCAGTACTTCCAATGCCTGAAGAGCAAGATTAGTATTTTTCtg                                                     <  1:310667/251‑1 (MQ=255)                                                                                                                                                                                     aaataaaGTGGTCACCATCATCACGCCAAGCCAATACCGGTAAATGTAAAAAAGGTAATCTATCAATTGTTTTGTTCGTAGTTCTTACTTTTAATCCAAGTGATCTTGCTGCAAGCAACCATTTAGTTTGGTTCAGCCCATGCCCATCTATATCAAATTTATGTTTTATTTCTTCAGGGTTAATTGAAATATTATGATATTGAGCCAGTACTTCCAATGCCTGAAg                                                                          >  1:481630/1‑226 (MQ=255)                                                                                                                                                                                       ataaaGTGGTCACCATCATCACGCCAAGCCAATACCGGTAAATGTAAAAAAGGTAATCTATCAATTGTTTTGTTCGTAGTTCTTACTTTTAATCCAAGTGATCTTGCTGCAAGCAACCATTTAGTTTGGTTCAGCCCATGCCCATCTatat                                                                                                                                                   <  1:502307/151‑1 (MQ=255)                                                                                                                                                                                                 cACCATCATCACGCCAAGCCAATACCGGTAAATGTAAAAAAGGTAATCTATCAATTGTTTTGTTCGTAGTTCTTACTTTTAATCCAAGTGATCTTGCTGCAAGCAACCATTTAGTTTGGTTCAGCCCATGCCCATCTATATCAAATTTATGTTTTATTTCTTCAGGGTTAATTGAAATATTATGATATTGAGCCAGTACTTCCAATGCCTGAAGAGCAAGATTAGTATTTTTCTGTGAGTCCATTACatat                                     >  1:262410/1‑251 (MQ=255)                                                                                                                                                                                                   ccATCATCACGCCAAGCCAATACCGGTAAATGTAAAAAAGGTAATCTATCAATTGTTTTGTTCGTAGTTCTTACTTTTAATCCAAGTGATCTTGCTGCAAGCAACCATTTAGTTTGGTTCAGCCCATGCCCATCTATATCAAATTTATGTTTTATTTCTTCAGGGTTAATTGAAATATTATGATATTGAGCCAGTACTTCCAATGCCTGAAGAGCAAGATTAGTATTTTTCTGTGAGTCCATTACATATcc                                   <  1:677991/251‑1 (MQ=255)                                                                                                                                                                                                   ccATCATCACGCCAAGCCAATACCGGTAAATGTAAAAAAGGTAATCTATCAATTGTTTTGTTCGTAGTTCTTACTTTTAATCCAAGTGATCTTGCTGCAAGCAACCATTTAGTTTGGTTCAGCCCATGCCCATCTATATCAAATTTATGTTTTATTTCTTCAGGGTTAATTGAAATATTATGATATTGAGCCAGTACTTCCAATGCCTGAAGAGCAAGATTAGTATTTTTCTGTGAGTCCATTACATATc                                    >  1:201576/1‑250 (MQ=255)                                                                                                                                                                                                                 agccaaTACCGGTAAATGTAAAAAAGGTAATCTATCAATTGTTTTGTTCGTAGTTCTTACTTTTAATCCAAGTGATCTTGCTGCAAGCAACCATTTAGTTTGGTTCAGCCCATGCCCATCTATATCAAATTTATGTTTTATTTCTTCAGGGTTAATTGAAATATTATGatat                                                                                                    >  1:745969/1‑172 (MQ=255)                                                                                                                                                                                                                 agccaaTACCGGTAAATGTAAAAAAGGTAATCTATCAATTGTTTTGTTCGTAGTTCTTACTTTTAATCCAAGTGATCTTGCTGCAAGCAACCATTTAGTTTGGTTCAGCCCATGCCCATCTATATCAAATTTATGTTTTATTTCTTCAGGGTTAATTGAAATATTATGATATTGAGCCAGTACTTCCAATGCCTGAAGAGCAAGATTAGTATTTTTCTGTGAGTCCATTAc                                         <  1:470863/231‑1 (MQ=255)                                                                                                                                                                                                                 agccaaTACCGGTAAATGTAAAAAAGGTAATCTATCAATTGTTTTGTTCGTAGTTCTTACTTTTAATCCAAGTGATCTTGCTGCAAGCAACCATTTAGTTTGGTTCAGCCCATGCCCATCTATATCAAATTTATGTTTTATTTCTTCAGGGTTAATTGAAATATTATGATATTGAGCCAGTACTTCCAATGCCTGAAGAGCAAGATTAGTATTTTTCTGTGAGTCCATTACATATCCTGAAAAACAGTGAt                     >  1:480436/1‑251 (MQ=255)                                                                                                                                                                                                                  gccaaTACCGGTAAATGTAAAAAAGGTAATCTATCAATTGTTTTGTTCGTAGTTCTTACTTTTAATCCAAGTGATCTTGCTGCAAGCAACCATTTAGTTTGGTTCAGCCCATGCCCATCTATATCAAATTTATGTTTTAttt                                                                                                                                 >  1:198015/1‑142 (MQ=255)                                                                                                                                                                                                                         ccGGTAAATGTAAAAAAGGTAATCTATCAATTGTTTTGTTCGTAGTTCTTACTTTTAATCCAAGTGATCTTGCTGCAAGCAACCATTTAGTTTGGTTCAGCCCATGCCCATCTATATCAAATTTATGTTTTATTTCTTCAGGGTTAATTGAAatat                                                                                                            <  1:421318/156‑1 (MQ=255)                                                                                                                                                                                                                                aTGTAAAAAAGGTAATCTATCAATTGTTTTGTTCGTAGTTCTTACTTTTAATCCAAGTGATCTTGCTGCAAGCAACCATTTAGTTTGGTTCAGCCCATGCCCATCTATATCAAATTTATGTTTTATTTCTTCAGGGTTAATTGAAATATTATGATATTGAGCCAGTACTTCCAATGcc                                                                               <  1:56080/178‑1 (MQ=255)                                                                                                                                                                                                                                aTGTAAAAAAGGTAATCTATCAATTGTTTTGTTCGTAGTTCTTACTTTTAATCCAAGTGATCTTGCTGCAAGCAACCATTTAGTTTGGTTCAGCCCATGCCCATCTATATCAAATTTATGTTTTATTTCTTCAGGGTTAATTGAAATATTATGATATTGAGCCAGTACTTCCAATGCCTGAAGAGCAAGATTAGTATTTTTCTGTGAGTCCATTACATATCCTGAAAAACAGTGATTCCACACATACAAat      <  1:421052/251‑1 (MQ=255)                                                                                                                                                                                                                                 tGTAAAAAAGGTAATCTATCAATTGTTTTGTTCGTAGTTCTTACTTTTAATCCAAGTGATCTTGCTGCAAGCAACCATTTAGTTTGGTTCAGCCCATGCCCATCTATATCAAATTTATGTTTTATTTCTTCAGGGTTAATTGAAATATTATGATATTGAGCCAGTACTTCCAATGCCTGAAGAGCAAGATTAGTATTTTTCTGTGAGTCCATTACATATCCTGAAAAACAGTGATTCCACACATACAAata     <  1:501623/251‑1 (MQ=255)                                                                                                                                                                                                                                    aaaaaaGGTAATCTATCAATTGTTTTGTTCGTAGTTCTTACTTTTAATCCAAGTGATCTTGCTGCAAGCAACCATTTAGTTTGGTTCAGCCCATGCCCATCTATATCAAATTTATGTTTTATTTCTTCAGGGTTAATTGAAATATTATGATATTGAGCCAGTACTTCCAATGCCTGAAGAGCAAGATTAGTATTTTTCTGTGAGTCCATTACATATCCTGAAAAACAGTGATTCCACACATACAAATATGc  <  1:7619/251‑1 (MQ=255)                                                                                                                                                                                                                                    aaaaaaGGTAATCTATCAATTGTTTTGTTCGTAGTTCTTACTTTTAATCCAAGTGATCTTGCTGCAAGCAACCATTTAGTTTGGTTCAGCCCATGCCCATCTATATCAAATTTATGTTTTATTTCTTCAGGGTTAATTGAAATATTATGATATTGAGCCAGTACTTCCAATGCCTGAAGAGCAAGATTAGTATTTTTCTGTGAGTCCATTACATATCCTGAAAAACAGTGATTCCACACATACAAATATGc  <  1:31942/251‑1 (MQ=255)                                                                                                                                                                                                                                         aggtaaTCTATCAATTGTTTTGTTCGTAGTTCTTACTTTTAATCCAAGTGATCTTGCTGCAAGCAACCATTTAGTTTGGTTCAGCCCATGCCCATCTATATCAAAt                                                                                                                                              >  1:245187/5‑106 (MQ=255)                                                                                                                                                                                                                                               tctatCAATTGTTTTGTTCGTAGTTCTTACTTTTAATCCAAGTGATCTTGCTGCAAGCAACCATTTAGTTTGGTTCAGCCCATGCCCATCTATATCAAATTTATGTTTTATTTCTTCAGGGTTAATTGAAATATTATGATATTGAGCCAGTACTTCCAATGCCTGAAGAGCAAGATTAGTATTTTTCTGTGAGTCCATTACATATCCTGAAAAAc                           <  1:129914/211‑1 (MQ=255)                                                                                                                                                                                                                                                 tatCAATTGTTTTGTTCGTAGTTCTTACTTTTAATCCAAGTGATCTTGCTGCAAGCAACCATTTAGTTTGGTTCAGCCCATGCCCATCTATATCAAATTTATGTTTTATTTCTTCAGGGTTAATTGAAat                                                                                                              <  1:858693/128‑1 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                        CAGGAATAAACCAGGTAAAGTCGAATTTTGCTAAATTTCCTACAATAGATGCGCGTGATGCAATTAAAATCACCTTGCTTTGATAACGTTCTTCAAATTCATTTTTGTCTAAAACAATAGGATTTTTTTGAATTAGATCAAAAATGAGGTAACGATCGGTTTCTTGATCTATTTTTAATAAAATAAAGTGTTCACCATCATCACGCCAAGCCAATGCCGGTAAATGTAAAAAAGGCAATCTGTCAACTGTTTTGTTCGCAGTTCTTACCTTTAATCCAAGTGATTTTGCTGCAAGCAACCATTTAGTTTGGTTCAGCCCATGCCCATCTATATCAAATTTATGTTTTATTTCTTCCGGGTTAATTGAAATATTATGATATTGAGCCAGTACTTCCAATGCCTGAAGGGCAAGATTAGTATTTTTCTGTGAGTCCATTACATATCCTGAAAAACAGTGATTCCGCACACACAAATATGC  >  1/1978455‑1978932

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑